BioProject

To study the community composition of picoeukaryotes during successive Noctiluca and Mesodinium blooms, 500 mL surface water samples were collected from Port Shelter, Hong Kong, pre-filtered through a 200 μm mesh, and further filtered through a 3. More...

To study the community composition of picoeukaryotes during successive Noctiluca and Mesodinium blooms, 500 mL surface water samples were collected from Port Shelter, Hong Kong, pre-filtered through a 200 μm mesh, and further filtered through a 3.0 µm (47 mm) polycarbonate membrane (PALL Corporation). Then water samples were filtered onto a 0.22 µm (47 mm) polycarbonate membrane for DNA extraction (GE Water and Process Technologies), using a vacuum pump at 0.034 bar Hg or below. The filters were flash frozen and stored at -80ºC until DNA extraction. Samples were then studied using 454-pyrosequencing Less...