BioProject

Protocorm-like body (PLB) formation is an efficient method that has been widely used in the propagation of orchids. More...

Protocorm-like body (PLB) formation is an efficient method that has been widely used in the propagation of orchids. There is little information regarding the genome-wide molecular spectrum of somaclonal variation in PLB-regenerated orchids. We used SLAF-seq to investigate the variations of SNPs and InDels in somaclones of Oncidium 'Milliongolds', which was regenerated via PLB formation after in vitro culturing of 10 years. The somaclonal lines included 5 plants with a normal phenotype (G1-G5), 5 mutants with whole yellow leaves (Y1 and Y2) or yellow-streaked leaves (Y3-Y5), and one mutant with increased leaf width and thickness (M). A flowering plant (N) of 'Milliongolds' purchased from a market was also used for comparative analysis. Less...