BioProject

Based on degradome sequencing, a total of 122.33 Mb of raw data was obtained from degradome sequencing analysis of six samples of YC05-179 and ROC22 (Y0, Y2, Y5, R0, R2, and R5) after inoculation. More...

Based on degradome sequencing, a total of 122.33 Mb of raw data was obtained from degradome sequencing analysis of six samples of YC05-179 and ROC22 (Y0, Y2, Y5, R0, R2, and R5) after inoculation. The Q30 of each sample was >93%, and the sequence used for degradation site analysis exactly matches the sugarcane reference sequence. A total of 309 target genes were detected in the six sugarcane samples, corresponding to 97 known miRNAs and 112 novel miRNAs, and 337 degradation sites were identified, indicating that miRNAs can efficiently cleave multiple sites of target genes. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that target genes were involved in various regulatory processes of life activity such as signal transduction mechanisms, inorganic ion transport and metabolism, defense mechanisms, translation, posttranslational modifications, energy production and conversion, and glycerolipid metabolism. A higher number of resistance-related target genes regulated by miRNA-mediated cleavage was accumulated in sugarcane during S. scitamineum infection, suggesting that feedback regulation of miRNAs is involved in the response of sugarcane to S. scitamineum infection. This study not only further promotes the elucidation of the underlying the response of sugarcane to S. scitamineum infection, but also provides a resource for miRNAs and their target genes for the improvement of smut-resistance in sugarcane varieties based on molecular biology techniques. Less...