BioProject

Mesothelium is a multipotent resident progenitor cell of the coelomic organs that functions in organogenesis, repair and possible regeneration. More...

Mesothelium is a multipotent resident progenitor cell of the coelomic organs that functions in organogenesis, repair and possible regeneration. We used hESCs to generate mesothelium of the epithelial and mesenchymal forms. Mesenchymal derivatives of mesothelium have been previously reported to function in tissue repair by promoting and participating in angiogenesis and neovascularization. We uncovered that hESC-derived mesothelium of the mesenchymal form (MesoT) are multipotent and generate smooth muscle cells, endothelial cells and pericytes and self-assemble into vessel-like networks in vitro. MesoT cells contribute to nascent coronary vessels in the repair zone of mechanically damaged neonatal mouse hearts. MesoT cells seeded onto vascular scaffolds self-assemble into vasculature capable of supporting peripheral blood flow following transplantation. Our findings demostrate the potential utility of MesoT cells in tissue repair and vascular engineering applications. Overall design: Profiles of mRNA transcripts levels were generated for hPSC-derived mesothelium and neonatal mouse by deep sequencing on an Illumina Hisec 2500 and Illumina NxtSeq 500 platform. Cells were generated from WA09 human embryonic stem cells (hESCs). Cell types include mesothelium of the epithelial form (MLC), mesothelium of the mesenchymal form (MesoT), mouse neonatal mesothelium isolated from lineage traced WT1 cells of the heart, lung, liver and gut, and hPSC-derived splanchnic mesoderm-derived smooth muscle cells to use as a comparison for PCA analysis. Less...