BioProject

Immunological Genome Project chromatin accessibility maps for 86 different immunocytes (ATAC-seq). More...

Immunological Genome Project chromatin accessibility maps for 86 different immunocytes (ATAC-seq). Immune cell populations were isolated in high-purity by flow cytometry. Overall design: Immune cell populations encompassing all major lineages were isolated by flow cytometry to high-purity according to ImmGen SOP (https://www.immgen.org/). 10,000 cells (except some rare cell populations) were used to construct each ATAC-seq library, using the Fast-ATAC protocol. Libraries were dual-indexed and pooled for high-throughput sequencing. Highly purified cell samples were isolated in parallel from the same populations for gene expression profiling by low-input RNAseq (ImmGen protocol, per umbrella GSE126267). These corresponding RNAseq datasets are available at GSE109125. -------------------------------------- Immunological Genome Project Consortium Less...