To exclude an intrinsic effect of the gene editing on cell growth,
we co-cultured FasKO B cells in vitro with 40LB cells to induce a GClike
phenotype in the B cells. More...
To exclude an intrinsic effect of the gene editing on cell growth,
we co-cultured FasKO B cells in vitro with 40LB cells to induce a GClike
phenotype in the B cells. FasWT B cells were co-cultured as a
control with the 40LB cells. We performed next-generation
amplicon sequencing on the extracted DNA from the iGC B cells
on days 3 (Wednesday), 4 (Thursday), and 5(Friday) to check for mutations in the Fas gene locus that
the crRNA had targeted. The frequency of mutations was then
compared to the flow cytometry knock-out efficiency. Less...