BioProject

RNA-Seq results for M. smegmatis wild type and napM-deleted strain, deprived of one of the nucleoid-associated proteins. More...

RNA-Seq results for M. smegmatis wild type and napM-deleted strain, deprived of one of the nucleoid-associated proteins. Next-generation sequencing results are provided in three independent biological replicates for each strain growing in standard 7H9/10% ADC medium. The bacteria were grown to mid-logarithmic phase of growth, when they were collected and total RNA was isolated. The resulting RNA was subjected to ribodepletion with riboPools Pan-Bacteria (siTools Biotech GmbH, Planegg, Germany) and used for the preparation of sequencing libraries, generated with a KAPA-stranded RNA Seq kit according to the manufacturer's protocol (Roche Diagnostics, Rotkreuz, Switzerland). The resulting adapter-ligated, PCR-amplified cDNA libraries were subjected to sequencing on a NextSeq500 sequencer using the NextSeq500/550 v.2 sequencing kit (Illumina, San Diego, CA, USA), and approximately 5 to 10 million paired-end reads were sequenced for each sample for total RNA-Seq. VAHTS mRNA-seq V3 Library Prep Kit for Illumina (Vazyme, Nanjing, China) with detailed manufacturer's protocol was applied to generate final sequencing libraries for the polyadenylation enriched RNA samples obtained from Mycolicibacterium smegmatis strains. Less...