Mycoplasma synoviae (MS) is a globally important avian pathogen that causes infectious synovitis, respiratory diseases, and eggshell apex abnormalities in chickens, leading to serious economic losses in the poultry industry.
More...Mycoplasma synoviae (MS) is a globally important avian pathogen that causes infectious synovitis, respiratory diseases, and eggshell apex abnormalities in chickens, leading to serious economic losses in the poultry industry. Vaccination is one of the most cost-effective and efficient methods for preventing and controlling MS infections. Therefore, developing new and efficient vaccines is important in reducing the risk of MS infection. In this study, six clinical MS strains were isolated from different provinces in China. After whole-genome sequencing, we analyzed their Common genes using Biopython software. We identified 22 genes that were shared among all strains; these genes had high copy numbers in highly virulent strains and, therefore, are potential antigen targets. Subsequently, by analyzing their properties, 10 candidate vaccine proteins were selected using Vaxign2 and IEDB Antibody Epitope Prediction. We then assessed the immunoprotective effects of these candidate proteins and explored the effects of two-component and multi-component antigen combinations to develop a more efficient vaccine. We successfully developed a multivalent subunit vaccine composed of MSPB, Ppht, Cfba, and EF-G, which displayed the best protective effect among all the candidate vaccines tested. The immunization dosage of this combination vaccine was 20ug, with each protein accounting for 25%. The immune production period of the subunit vaccine is determined to be 28 days after the first immunization, with a duration of 180 days, and the immune protection rate against highly virulent strains reached 90-100%. These findings demonstrate that the combination of MSPB, Ppht, Cfba, and EF-G can be an efficient multi-component subunit vaccine, providing a new approach for developing vaccines against MS.
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