Multidrug resistant bacteria is an agent responsible for nosocomial infections with high morbidity and mortality, due to the ease of horizontal transfer of mobile genetic elements such as plasmids that confer resistance genes and virulence factors, thus facilitating the production of Beta enzymes extended spectrum lactamases (ESBL) and carbapenemases.
More...Multidrug resistant bacteria is an agent responsible for nosocomial infections with high morbidity and mortality, due to the ease of horizontal transfer of mobile genetic elements such as plasmids that confer resistance genes and virulence factors, thus facilitating the production of Beta enzymes extended spectrum lactamases (ESBL) and carbapenemases. Despite the greater predominance of Kpn MDR in hospital reports in Peru, in Cusco very few strains have been characterized and described genomically.
From 2022 to 2023, 91 strains of bacteria MDR at Cusco were isolated, coming from three hospitals in Cusco. The phenotypic detection of ESBL was performed by the Jarlier method and carbapenemases by double disk synergy with Boronic Acid and EDTA. Isolates with positive results were analyzed by endpoint PCR for genes blaCTX M, TEM, and blaSHV, to ESBL and blaKPC, NDM, IMP, and blaVIM of carbapenemases. Additionally, 4 isolates were randomly selected for whole genome sequencing (NGS) Less...