During the subculture of filamentous fungi, obvious signs of degradation will occur,which will affect the growth and development of the strain, change the content of metabolites and interfere with gene expression.
More...During the subculture of filamentous fungi, obvious signs of degradation will occur,which will affect the growth and development of the strain, change the content of metabolites and interfere with gene expression.However, the specific molecular mechanism of filamentous fungi degradation is still unclear.In this study, a filamentous fungus Samsoniella hepiali was used as the research object, and it was continuously subcultured. The results showed that when the strain was subcultured to the F8 generation, the strain began to show signs of degradation, which was manifested in affecting the apparent morphology, reducing the growth rate and sporulation, and destroying the antioxidant system. Further transcriptome and metabolomics analysis were performed, and the results showed, differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were mainly enriched in four metabolic pathways: ABC transporters, fatty acid degradation, al-anine, aspartate and glutamate metabolism, and purine metabolism. Many of the metabolites significantly enriched in different pathways may be mainly regulated by genes belonging to proteins and enzymes, such as Abcd3, Ass1 and Pgm1. At the same time, in the process of sub-culture, many genes and metabolites that can induce apoptosis and senescence continue to ac-cumulate, causing cell damage and consuming a lot of energy, which ultimately leads to the in-hibition of mycelial growth. In summary, this study clarified the response of S. hepiali strains to key metabolic pathways during subculture and some reasons for the degradation of strains.
Less...