BioProject

Small RNA sequencing was performed in the paired primary glioblastoma cell lines derived from patients tissue. More...

Small RNA sequencing was performed in the paired primary glioblastoma cell lines derived from patients tissue. Each pair comprise of glioblastoma stem-like cell counterpart (propagated in DMEM/F12 cultivation medium (Gibco) with 2% B-27 50X Supplement, 1% Glutamax (all ThermoFisher Scientific), 100 U/ml penicillin, and 100 ug/ml streptomycin (both GE Healthcare), and non-stem cell counterpart (propagated in DMEM (Gibco) supplemented with 10% FBS, 1% Glutamax (both ThermoFisher Scientific), 100 U/ml penicillin and 100 ug/ml streptomycin, 1mM sodium pyruvate, and 1% non-essential amino acids (all GE Healthcare)). RNA isolated from cell lines was used for the identification of piRNA molecules that were dysregulated in glioblastom stem cells compared to non-stem cells. Less...