BioProject

This mesocosm experiment investigated the impact of inoculating soybean with rhizobia on soil microbiomes sampled from two different fields on a single UK farm. More...

This mesocosm experiment investigated the impact of inoculating soybean with rhizobia on soil microbiomes sampled from two different fields on a single UK farm. One soil had no history of previous soybean cultivation/ inoculation and the other had soybean grown and inoculated with a commercial inoculant (containing B. japonicum SEMIA 5079 and B. diazoefficiens SEMIA 5080) in the season prior. An experiment was designed with each soil (no-soya and soya) split into 4 inoculation treatments: a control mock-inoculated treatment, a control supplemented with Nitrogen fertiliser, a Bradyrhizobium diazoefficiens inoculant and a Sinorhizobium fredii inoculant treatment each with 5 replicates. Starting microbiome samples of each bulk soil were taken on the day of planting (T0). Soil microbiomes from pots were repeatedly sampled at 2 days post treatment (seedling emergence - VE), 22 dpt (vegetative growth stages V2 -V3), 63 dpt (pod formation R3- R5) and 84 dpt (green pod fill R6), when plants were harvested. Soil samples were snap frozen in liquid nitrogen before storing at -80 degrees C, DNA was extracted from 500 mg of soil. Seven plant root nodules were excised from roots at harvest, sterilised, crushed and 100 microlitre extract taken and stored at -80 degrees C until DNA extractions. Amplicons were generated for 16S rRNA (Caporaso et al ., 2012) and rpoB (alphaproteobacteria - Wardell G. Thesis 2024) for soil DNA and rpoB for nodule DNA. Less...