Myocardial ischemia-reperfusion injury (MIRI) is a common and serious complication of percutaneous coronary intervention, which is a key factor affecting the long-term prognosis. Mitochondrial quality control (MQC) is very important for MIRI pathological process. Previous studies have demonstrated that shuangshen ningxin (SSNX) can reduce myocardial ischemia and infarct size, protect heart function, reduce cardiomyocyte apoptosis, and regulate cardiomyocyte autophagy.Further studies have found that SSNX can regulate cardiomyocyte mitochondrial autophagy, PINK1/Parkin, Nix and other pathway proteins. In conclusion, we propose a hypothesis that SSNX can effectively protect myocardium from MIRI by regulating mitochondrial division and fusion, biogenesis and autophagy to maintain mitochondrial and cell homeostasis. The purpose of this project is to detect the ultrastructure, morphology, function, mitochondrial membrane potential, mitochondrial reactive oxygen, and the expression of Fis1, Drp1, Mfn1 / 2, Opa1, AMPK, PGC-1 α, NRF, TFAM and other proteins in myocardial cells at the animal tissue cell molecular level by using living cell workstation, transmission electron microscope , flow cytometry and application of agonists and inhibitors, so as to clarify the regulation of SSNX on MQC in the prevention and treatment of MIRI.
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