BioProject

The goal of this project is to identify Arabidopsis CLE signaling downstream genes involved in syncytium formation during beet cyst nematode (Heterodera schachtii) infection. More...

The goal of this project is to identify Arabidopsis CLE signaling downstream genes involved in syncytium formation during beet cyst nematode (Heterodera schachtii) infection.

Wild-type (Col-0) and clv1-101 clv2-101 rpk2-5 seedlings were grown on modified Knops media in square plates (100 x 100 x 15 mm) vertically for 7 days. The growth chamber was set at 24 degree Celsius with 12 h light/12 h dark light cycle. About 10-15 sterilized BCN (H.schachtii) pre-parasitic 2nd stage juveniles were inoculated to each root. Developing syncytium (about 8 mm root segments) were collected from primary roots at 4 dpi (days-post-inoculation) under stereoscope and immediately frozen in liquid nitrogen.

Samples treated with HsCLE2 peptide were also included to filter out genes specifically response to the HsCLE2 peptide effector. 11-day-old seedlings grown in square plates were treated by covering all roots by adding 3 ml of 5 uM HsCLE2 solution to the plate. Plates were then kept in the growth chamber for 3 hours. Root segments corresponding to BCN infection sites (about 8 mm) were collected under stereoscope and immediately frozen in liquid nitrogen for RNA isolation. Less...