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3 additional projects are components of the Titration of RAS alters senescent state and influences tumour initiation.
Single cell sequencing of RPE1-hTert cells transduced with the NLS-mVenus-P2A-ER:HRAS^G12V construct, and then flow-sorted into subpopulations based on increasing mVenus intensity, corresponding to different levels of oncogenic RAS stabilised upon induction with 4OHT.
Overall design: RPE1-hTert cells were infected with the predictive reporter (NLS-mVenus-P2A-ER:HRAS^G12V) construct then flow sorted into subpopulations based on increasing mVenus intensity ('S' < 'M' < 'L' < 'XL'). Cells were grown either in monoculture i.e. each subpopulation separately, or in coculture i.e. subpopulations mixed together in equal proportions, and induced with 4OHT. A total of 6 samples (uninduced coculture, induced coculture, induced 'S', induced 'M', induced 'L' and induced 'XL') were hashtagged with TotalSeq-A hashtag antibodies and pooled for encapsulation and single-cell sequencing using the 10x Genomics platform. Induced samples were harvested on Day 6 post-induction.
| Accession | PRJNA1049380; GEO: GSE249489 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Homo sapiens[Taxonomy ID: 9606] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo; Homo sapiens |
| Publications | Chan ASL et al., "Titration of RAS alters senescent state and influences tumour initiation.", Nature, 2024 Sep;633(8030):678-685 |
| Submission | Registration date: 6-Dec-2023
Narita Lab, Cancer Research UK, University of Cambridge |
| Relevance | Medical |
Project Data:
| Resource Name | Number
of Links |
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| Sequence data |
| SRA Experiments | 2 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 2 |
| GEO DataSets | 1 |