BioProject

The experiment tested organ-specific responses of rice (Oryza sativa ssp. japonica) to cold stress with a special focus on phytohormonal regulation. More...

The experiment tested organ-specific responses of rice (Oryza sativa ssp. japonica) to cold stress with a special focus on phytohormonal regulation. Cold stress (5°C, 24 h) was applies on the whole plants, leaves or roots. The results showed distinct responses when cold stress was applied on leaves, relating to photosynthesis and sugar synthesis as well as specific changes in phytohormones. On the other hand, stress applied to roots was more similar to the stress on the whole plant indicating roots to be more important in cold stress responses. Acclimation by mild temperature (15°C, 12 h) highlighted changes which are connected even with lower temperature exposure or which are characteristic for untreated organs. Recovery (3 d) indicated ability of plants to restore growth which correlated between individual phytohormones and plant growth. The article connect transcriptome, hormonome, proteome and sugar analyses of rice cold-stress responses. Overall design: Comparative gene expression profiling analysis of RNA-seq data from roots of Oryza sativa seedlings grown 13 days in hydroponics, treated for 24 h by cold stress (5°C, 24 h) targeted to leaves, roots or the whole plant. Some plants were pre-treated by acclimation (15°C, 12 h). Some samples were collected after 3-day recovery under control conditions. Three independent biological replicates were collected. Less...