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IDs: 535528 [UID] 532628 [GenBank] 535528 [RefSeq]
Streptococcus pneumoniae is a member of the Firmicutes division of the domain bacteria and has been isolated from a blood sample taken from a clinical pediatric case of invasive pneumococcal disease. This is a reference genome for the Human Microbiome ... Project. This project is co-owned with the Human Microbiome Project DACC. Source DNA provided by Martin Antonio, Ph.D. (Medical Research Council Laboratories, The Gambia:mantonio@mrc.gm). Bacteria provided by Martin Antonio, Ph.D. (mantonio@mrc.gm), as part of the "Determining the genome sequence of a Gambian isolate of Streptococcus pneumoniae serotype 1 ST618" project (L2007.30), funded by Medical Research Council, UK. Draft assembly of Streptococcus pneumoniae PNI0153, generated by Newbler assembler, was finished to a high quality standard by resolving ambiguous bases, correcting misassembled regions and filling the gaps with targeted PCR and sequencing. Coding sequences were predicted using GeneMark v3.3 and Glimmer2 v2.13. Intergenic regions not spanned by GeneMark and Glimmer2 were blasted against NCBI's non-redundant (NR) database and predictions generated based on protein alignments. tRNA genes were determined using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and Rfam v8.0. Gene names are generated at the contig level and may not necessarily reflect any known order or orientation between contigs. more
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