Requirements for GenBank Submissions through BankIt
- Contact Information
- Name, address, phone number, fax number and email address of the submitter must be entered when registering and submitting for the first time
- Subsequent BankIt submissions will retain this information and display it once the submitter logs in
- Release date information
- Immediately after it is processed at NCBI OR
- On a date the submitter specifies
- Reference information
- Sequence authors: names of the researchers who are credited with the sequence
- Publication information: Unpublished, In-Press, or Published; and applicable citation information (paper's title, authors, journal title, volume, issue, year, pages)
- Submission Category and Type
- Original sequencing or Third Party Annotation
- Single sequence, sequence set (phylogenetic, population, environmental, etc), or batch
- Nucleotide sequence(s)
- Input (cut-and-paste) single or multiple sequences OR
- Upload them as a FASTA file; FASTA files should include organisms in their definition lines
- Sequences must be at least 200 nucleotides long (unless they are complete exons, non-coding RNAs (ncRNAs), microsatellites or ancient DNA)
- Molecule type: what was sequenced? (genomic DNA, mRNA, genomic RNA, cRNA, etc)
- Topology: linear or circular (circular must be complete, such as a complete plasmid)
- Organism name, applicable source modifiers, location
- Genus and species names (if not previously provided in FASTA file)
- If name is new or unrecognized, provide best known taxonomic lineage
- If genus and/or species names are not known, provide most specific name known (for example:Bacillus sp., Uncultured bacterium, Uncultured archaeon)
- Most complete name for any synthetic vector (for example: Cloning vector pAB234, Transfer vector p789Abc)
- Source modifiers include: strain, clone, isolate, specimen-voucher, isolation-source, country
- Location: organelle (mitochondrion, chloroplast, etc); map and/or chromosome
- Features of the sequence
- Upload files or use input forms to add all applicable features (for example: CDS, gene, rRNA, tRNA, microsatellite, exon, intron)