|
Thauera chlorobenzoica Thauera chlorobenzoica is a gram negative short rod with a length of 3 to 3.7 m when growing on MR2A plate aerobically. It is motile during early stages of growth with peritrichous flagellation. Oxidase and catalase are positive. Nitrate, nitrite, and oxygen are used as the terminal electron acceptors. 3-Chlorobenzoate, 3-bromobenozate and 3-iodobenzoate are utilized as growth substrates under denitrifying conditions. In addition, strain 3CB-1 uses benzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, 2-fluorobenozate, and 4-fluorobenozate under both aerobic and anaerobic conditions and benzyl alcohol, caproate, 3-aminobenzoate, protochatechuate, 3-methylphenol and 4-methylphenol only under denitrifying condtions. It can tolerate 2 % NaCl under denitrifying conditions and cannot hydrolyze gelatin, starch, and urea. The predominant fatty acids are cis-7-hexadecenoic acid (16:1w7c), hexadecanoic acid (16:0) and Octadecenoic acid (18:0). During prolonged incubations under denitrifying conditions the relative protion of methylene-hexadecanoic acid (17:0 CYCLO) increases with corresponding decrease in cis-7-hexadecenoic acid (16:1w7c). Strain 3CB-1 belongs to the genus Thauera in the beta subunit of Proteobacteria on the basis of cellular fatty acid analysis, 16S rRNA gene sequence analysis and DNA-DNA hybridization. Strain 3CB-1 is the type strain of the species Thauera chlorobenzoica. Bongkeun Song, personal communication.
|