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Conserved domains on  [gi|158296906|ref|XP_317236|]
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AGAP008234-PA [Anopheles gambiae str. PEST]

Protein Classification

nuclear transport factor 2 family protein( domain architecture ID 10092288)

nuclear transport factor 2 (NTF2) is a small guanosine 5'-diphosphate (GDP) Ran binding protein which facilitates transport of certain proteins into the nucleus to regulate multiple processes, including cell cycle, immunoreactions, and apoptosis.

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NTF2 cd00780
Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of ...
 16-137 7.30e-39

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.


:

Pssm-ID: 238403  Cd Length: 119  Bit Score: 127.40  E-value: 7.30e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158296906  16 TVCTTAEAFVKLYYDHVDKKRQHMSRLYMDTGLLVWNG-NGAKGKDEIQKYFHELP--RSEHTITTLDAQPIVDDAVssq 92
Cdd:cd00780    1 SAEDVAKAFVQQYYSIFDNNREGLHRLYGDTSMLSREGmKQVTGRDAIVEKLSSLPfqKTKHKITTVDSQPTPSGGV--- 77

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 158296906  93 ltfVMQVSGTVRFQENPTKPFQQTFMITAQGDKWKIVSDCFRLQD 137
Cdd:cd00780   78 ---IVMVTGSLKLDEQPPRKFSQTFVLAPQNGGYFVLNDIFRFVD 119
 
Name Accession Description Interval E-value
NTF2 cd00780
Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of ...
 16-137 7.30e-39

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.


Pssm-ID: 238403  Cd Length: 119  Bit Score: 127.40  E-value: 7.30e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158296906  16 TVCTTAEAFVKLYYDHVDKKRQHMSRLYMDTGLLVWNG-NGAKGKDEIQKYFHELP--RSEHTITTLDAQPIVDDAVssq 92
Cdd:cd00780    1 SAEDVAKAFVQQYYSIFDNNREGLHRLYGDTSMLSREGmKQVTGRDAIVEKLSSLPfqKTKHKITTVDSQPTPSGGV--- 77

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 158296906  93 ltfVMQVSGTVRFQENPTKPFQQTFMITAQGDKWKIVSDCFRLQD 137
Cdd:cd00780   78 ---IVMVTGSLKLDEQPPRKFSQTFVLAPQNGGYFVLNDIFRFVD 119
NTF2 pfam02136
Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like ...
 21-135 8.21e-24

Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like Delta-5-3-ketosteroid isomerase proteins.


Pssm-ID: 396625  Cd Length: 116  Bit Score: 88.95  E-value: 8.21e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158296906   21 AEAFVKLYYDHVDK-KRQHMSRLY-MDTGLLVWNGNGA-KGKDEIQKYFHELP--RSEHTITTLDAQPivddAVSSQLTF 95
Cdd:pfam02136   2 GNAFVQQYYAALDAhDPEGLHALYaDDASMLTPPGSDPvVGLEAINEFFDSLPftRIQHKITSVDSQP----ASLKDGVL 77

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 158296906   96 VMqVSGTVRFQENPTKPFQQTFMITAQGDKWKIVSDCFRL 135
Cdd:pfam02136  78 VM-VTGELTVDDFPRRRFSQTFLLAPQRGGYFVLNDIFRY 116
 
Name Accession Description Interval E-value
NTF2 cd00780
Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of ...
 16-137 7.30e-39

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.


Pssm-ID: 238403  Cd Length: 119  Bit Score: 127.40  E-value: 7.30e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158296906  16 TVCTTAEAFVKLYYDHVDKKRQHMSRLYMDTGLLVWNG-NGAKGKDEIQKYFHELP--RSEHTITTLDAQPIVDDAVssq 92
Cdd:cd00780    1 SAEDVAKAFVQQYYSIFDNNREGLHRLYGDTSMLSREGmKQVTGRDAIVEKLSSLPfqKTKHKITTVDSQPTPSGGV--- 77

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 158296906  93 ltfVMQVSGTVRFQENPTKPFQQTFMITAQGDKWKIVSDCFRLQD 137
Cdd:cd00780   78 ---IVMVTGSLKLDEQPPRKFSQTFVLAPQNGGYFVLNDIFRFVD 119
NTF2 pfam02136
Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like ...
 21-135 8.21e-24

Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like Delta-5-3-ketosteroid isomerase proteins.


Pssm-ID: 396625  Cd Length: 116  Bit Score: 88.95  E-value: 8.21e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158296906   21 AEAFVKLYYDHVDK-KRQHMSRLY-MDTGLLVWNGNGA-KGKDEIQKYFHELP--RSEHTITTLDAQPivddAVSSQLTF 95
Cdd:pfam02136   2 GNAFVQQYYAALDAhDPEGLHALYaDDASMLTPPGSDPvVGLEAINEFFDSLPftRIQHKITSVDSQP----ASLKDGVL 77

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 158296906   96 VMqVSGTVRFQENPTKPFQQTFMITAQGDKWKIVSDCFRL 135
Cdd:pfam02136  78 VM-VTGELTVDDFPRRRFSQTFLLAPQRGGYFVLNDIFRY 116
NTF2_like cd00531
Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 ...
 21-136 1.90e-13

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.


Pssm-ID: 238296 [Multi-domain]  Cd Length: 124  Bit Score: 62.53  E-value: 1.90e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158296906  21 AEAFVKLYYDHVDKK-RQHMSRLYMDTGLLVWNGNGA-----KGKDEIQKYFHELP----RSEHTITTLDAQPIVDDAVS 90
Cdd:cd00531    1 AEQFLYRYARLLDAGdREWLALLYADDAYFEPPGGDGliypdDGREAIEDRVRRLPfgpsRTRHLVSNVDVQPGDDGEGV 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 158296906  91 sqltfVMQVSGTVRFQENPTKP---FQQTFMITAQGDKWKIVSDCFRLQ 136
Cdd:cd00531   81 -----VVSVFGVLRTRGDGEQDvfaGGQTFVLRPQGGGGKIANRRFRLD 124
Mtr2 pfam10429
Nuclear pore RNA shuttling protein Mtr2; Mtr2 is a monomeric, dual-action, RNA-shuttle protein ...
 21-125 2.95e-04

Nuclear pore RNA shuttling protein Mtr2; Mtr2 is a monomeric, dual-action, RNA-shuttle protein found in yeasts. Transport across the nuclear-cytoplasmic membrane is via the macro-molecular membrane-spanning nuclear pore complex, NPC. The pore is lined by a subset of NPC members called nucleoporins that present FG (Phe-Gly) receptors, characteriztically GLFG and FXFG motifs, for shuttling RNAs and proteins. RNA cargo is bound to soluble transport proteins (nuclear export factors) such as Mex67 in yeasts, and TAP in metazoa, which pass along the pore by binding to successive FG receptors. Mtr2 when bound to Mex67 maximizes this FG-binding. Mtr2 also acts independently of Mex67 in transporting the large ribosomal RNA subunit through the pore.


Pssm-ID: 402175  Cd Length: 164  Bit Score: 38.66  E-value: 2.95e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158296906   21 AEAFVKLYYDHVD-----KKRQHMSRLYMDTGLLVWNGNGAKGKDE-IQKYFHELPRSEHTITTLDAQPIVDDAvssqlT 94
Cdd:pfam10429   7 TETFLKKILAHLDeqdpnKLEQFLQLFLPPQCAIVFNGTPFAQPDAfLQMWLQQVVLTQHDLTGFDYHVIPGTG-----T 81

                          90       100       110
                  ....*....|....*....|....*....|.
gi 158296906   95 FVMQVSGTVRFQENPTKPFQQTFMITAQGDK 125
Cdd:pfam10429  82 LICNANCKVRFDESGRDKMGQDSTLPAPGAN 112
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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