NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1958746975|ref|XP_038953797|]
View 

centromere protein N isoform X1 [Rattus norvegicus]

Protein Classification

CENP-N/CHL4 family protein( domain architecture ID 10525992)

CENP-N/CHL4 family protein similar to fungal inner kinetochore subunit CHL4 and vertebrate centromere protein N, a component of the complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

Name Accession Description Interval E-value
CENP-N pfam05238
Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a ...
4-340 4.81e-112

Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a component of the central kinetochore which mediates the attachment of the centromere to the mitotic spindle. CENP-N is one of the components that assembles onto the CENP-A-nucleosome-associated (NAC) centromere. The centromere, which is the basic element of chromosome inheritance, is epigenetically determined in mammals. CENP-A, the centromere-specific histone H3 variant, assembles an array of nucleosomes and it is this that seems to be the prime candidate for specifying centromere identity. CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of CENP-M, CENP-N and CENP-T, CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Additionally, there are seven other subunits which make up the CENP-A-nucleosome distal (CAD) centromere, CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S, also assembling on the CENP-A NAC.


:

Pssm-ID: 398764  Cd Length: 403  Bit Score: 331.33  E-value: 4.81e-112
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975   4 NVAEFLRRIILKIPLCEMKTILEAW------------------DFLSEDQLQSINL------KQRKEFLAQEVILLCEDK 59
Cdd:pfam05238   1 STTPVLRRILLRLPRSSLLDLLQKWldkrntqpiddgddededGFLPERTLQTLRElyqelqKGPKREVIDRILEGDWPH 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975  60 CASLDDMALLDLMYTQFHRHQKLWNVFQMSKEPGGNDVDLFDMEQFQSSFKRILQRALKNVTVSFRVYEKNSVWIRV-AW 138
Cdd:pfam05238  81 GLNLYQLAQLDIQYLVDHPNSLRWTASQLSDSDGKEVIPRLDPSTFLSNLQEQLSPLYKAHYYLFRHPELPLIIIRIqLF 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975 139 GTQYSQPNQ--------YKPTFVVYYPQTPYVFIsSCYLKSTVPLLHQALKVA--------SKHHQMAQLDLRSRHLDSL 202
Cdd:pfam05238 161 DSPYSTPNQapeplldsSKPFYVAFPDNSPYVFI-SSHSPATDPLLLQALVLQavpralsrSKRIQLKPTSLPTRSLDAL 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975 203 TAIV-FNEYDQTFENYNSTA--SWREGSLGLKTD----------------------LDSKIIHENTEEKVRIHR--ATQE 255
Cdd:pfam05238 240 LALRgPSRYNQAMGAWSIYAdaSVEGSPLGPPPKhpiapgkrkgdhedkdpgsgsgVDSRIIYENKRTKERVHRklTAQE 319
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975 256 AFGAY-PQPQLEFAQYKLETKFKSDVgGGILADRKEPLRCLVKFSSPHLLEALKSLAPAGIADTPLSPLLTCiPSKKMNY 334
Cdd:pfam05238 320 RFGTYdSLPPLERVEYTLEDPFKSDS-GGYNSDRKEPFRIKLKFSGPDVFAGLRELADAGIIDPPKMPGWLT-GEEGVSS 397

                  ....*.
gi 1958746975 335 FKIRDK 340
Cdd:pfam05238 398 GKVRDG 403
 
Name Accession Description Interval E-value
CENP-N pfam05238
Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a ...
4-340 4.81e-112

Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a component of the central kinetochore which mediates the attachment of the centromere to the mitotic spindle. CENP-N is one of the components that assembles onto the CENP-A-nucleosome-associated (NAC) centromere. The centromere, which is the basic element of chromosome inheritance, is epigenetically determined in mammals. CENP-A, the centromere-specific histone H3 variant, assembles an array of nucleosomes and it is this that seems to be the prime candidate for specifying centromere identity. CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of CENP-M, CENP-N and CENP-T, CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Additionally, there are seven other subunits which make up the CENP-A-nucleosome distal (CAD) centromere, CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S, also assembling on the CENP-A NAC.


Pssm-ID: 398764  Cd Length: 403  Bit Score: 331.33  E-value: 4.81e-112
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975   4 NVAEFLRRIILKIPLCEMKTILEAW------------------DFLSEDQLQSINL------KQRKEFLAQEVILLCEDK 59
Cdd:pfam05238   1 STTPVLRRILLRLPRSSLLDLLQKWldkrntqpiddgddededGFLPERTLQTLRElyqelqKGPKREVIDRILEGDWPH 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975  60 CASLDDMALLDLMYTQFHRHQKLWNVFQMSKEPGGNDVDLFDMEQFQSSFKRILQRALKNVTVSFRVYEKNSVWIRV-AW 138
Cdd:pfam05238  81 GLNLYQLAQLDIQYLVDHPNSLRWTASQLSDSDGKEVIPRLDPSTFLSNLQEQLSPLYKAHYYLFRHPELPLIIIRIqLF 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975 139 GTQYSQPNQ--------YKPTFVVYYPQTPYVFIsSCYLKSTVPLLHQALKVA--------SKHHQMAQLDLRSRHLDSL 202
Cdd:pfam05238 161 DSPYSTPNQapeplldsSKPFYVAFPDNSPYVFI-SSHSPATDPLLLQALVLQavpralsrSKRIQLKPTSLPTRSLDAL 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975 203 TAIV-FNEYDQTFENYNSTA--SWREGSLGLKTD----------------------LDSKIIHENTEEKVRIHR--ATQE 255
Cdd:pfam05238 240 LALRgPSRYNQAMGAWSIYAdaSVEGSPLGPPPKhpiapgkrkgdhedkdpgsgsgVDSRIIYENKRTKERVHRklTAQE 319
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975 256 AFGAY-PQPQLEFAQYKLETKFKSDVgGGILADRKEPLRCLVKFSSPHLLEALKSLAPAGIADTPLSPLLTCiPSKKMNY 334
Cdd:pfam05238 320 RFGTYdSLPPLERVEYTLEDPFKSDS-GGYNSDRKEPFRIKLKFSGPDVFAGLRELADAGIIDPPKMPGWLT-GEEGVSS 397

                  ....*.
gi 1958746975 335 FKIRDK 340
Cdd:pfam05238 398 GKVRDG 403
 
Name Accession Description Interval E-value
CENP-N pfam05238
Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a ...
4-340 4.81e-112

Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a component of the central kinetochore which mediates the attachment of the centromere to the mitotic spindle. CENP-N is one of the components that assembles onto the CENP-A-nucleosome-associated (NAC) centromere. The centromere, which is the basic element of chromosome inheritance, is epigenetically determined in mammals. CENP-A, the centromere-specific histone H3 variant, assembles an array of nucleosomes and it is this that seems to be the prime candidate for specifying centromere identity. CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of CENP-M, CENP-N and CENP-T, CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Additionally, there are seven other subunits which make up the CENP-A-nucleosome distal (CAD) centromere, CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S, also assembling on the CENP-A NAC.


Pssm-ID: 398764  Cd Length: 403  Bit Score: 331.33  E-value: 4.81e-112
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975   4 NVAEFLRRIILKIPLCEMKTILEAW------------------DFLSEDQLQSINL------KQRKEFLAQEVILLCEDK 59
Cdd:pfam05238   1 STTPVLRRILLRLPRSSLLDLLQKWldkrntqpiddgddededGFLPERTLQTLRElyqelqKGPKREVIDRILEGDWPH 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975  60 CASLDDMALLDLMYTQFHRHQKLWNVFQMSKEPGGNDVDLFDMEQFQSSFKRILQRALKNVTVSFRVYEKNSVWIRV-AW 138
Cdd:pfam05238  81 GLNLYQLAQLDIQYLVDHPNSLRWTASQLSDSDGKEVIPRLDPSTFLSNLQEQLSPLYKAHYYLFRHPELPLIIIRIqLF 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975 139 GTQYSQPNQ--------YKPTFVVYYPQTPYVFIsSCYLKSTVPLLHQALKVA--------SKHHQMAQLDLRSRHLDSL 202
Cdd:pfam05238 161 DSPYSTPNQapeplldsSKPFYVAFPDNSPYVFI-SSHSPATDPLLLQALVLQavpralsrSKRIQLKPTSLPTRSLDAL 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975 203 TAIV-FNEYDQTFENYNSTA--SWREGSLGLKTD----------------------LDSKIIHENTEEKVRIHR--ATQE 255
Cdd:pfam05238 240 LALRgPSRYNQAMGAWSIYAdaSVEGSPLGPPPKhpiapgkrkgdhedkdpgsgsgVDSRIIYENKRTKERVHRklTAQE 319
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958746975 256 AFGAY-PQPQLEFAQYKLETKFKSDVgGGILADRKEPLRCLVKFSSPHLLEALKSLAPAGIADTPLSPLLTCiPSKKMNY 334
Cdd:pfam05238 320 RFGTYdSLPPLERVEYTLEDPFKSDS-GGYNSDRKEPFRIKLKFSGPDVFAGLRELADAGIIDPPKMPGWLT-GEEGVSS 397

                  ....*.
gi 1958746975 335 FKIRDK 340
Cdd:pfam05238 398 GKVRDG 403
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH