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Conserved domains on  [gi|1907114272|ref|XP_036015492|]
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mitochondrial tRNA-specific 2-thiouridylase 1 isoform X5 [Mus musculus]

Protein Classification

adenine nucleotide alpha hydrolase family protein( domain architecture ID 188)

AANH (adenine nucleotide alpha hydrolase) family protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AANH_superfamily super family cl00292
Adenine nucleotide alpha hydrolase (AANH) superfamily; The adenine nucleotide alpha hydrolase ...
 71-150 7.42e-30

Adenine nucleotide alpha hydrolase (AANH) superfamily; The adenine nucleotide alpha hydrolase (AANH) superfamily includes N-type ATP PPases, ATP sulfurylases, universal stress response proteins (USPs), and electron transfer flavoproteins (ETFs). The domain forms an alpha/beta/alpha fold which binds to adenosine nucleotide.


The actual alignment was detected with superfamily member cd01998:

Pssm-ID: 469708 [Multi-domain]  Cd Length: 349  Bit Score: 113.75  E-value: 7.42e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907114272  71 TVSQDALRRTIFPLGELTKDFVKKIAAENSLhHVLQKRESMGICFIGKRNLEHFLLQYLQPR-PGKFVSIeDNTVLGTHK 149
Cdd:cd01998   152 RLSQEQLSRTLFPLGHLTKSEVREIAREAGL-PVAEKKDSQGICFIGKRDFRDFLKEYLPEKlPGPIVDI-DGKVLGEHK 229


                  .
gi 1907114272 150 G 150
Cdd:cd01998   230 G 230
 
Name Accession Description Interval E-value
MnmA_TRMU-like cd01998
MnmA/TRMU family 2-thiouridylases and similar proteins; This family is composed of bacterial ...
 71-150 7.42e-30

MnmA/TRMU family 2-thiouridylases and similar proteins; This family is composed of bacterial tRNA-specific 2-thiouridylase MnmA (EC 2.8.1.13) and mitochondrial tRNA-specific 2-thiouridylase 1 (TRMU or MTU1, EC 2.8.1.14). MnmA catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. TRMU/MTU1 catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln); this is required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. This family belongs to the adenine nucleotide alpha hydrolase (AANH) superfamily that also includes other N-type ATP PPases and ATP sulfurylases. It forms an alpha/beta/alpha fold which binds to the adenosine group.


Pssm-ID: 467502 [Multi-domain]  Cd Length: 349  Bit Score: 113.75  E-value: 7.42e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907114272  71 TVSQDALRRTIFPLGELTKDFVKKIAAENSLhHVLQKRESMGICFIGKRNLEHFLLQYLQPR-PGKFVSIeDNTVLGTHK 149
Cdd:cd01998   152 RLSQEQLSRTLFPLGHLTKSEVREIAREAGL-PVAEKKDSQGICFIGKRDFRDFLKEYLPEKlPGPIVDI-DGKVLGEHK 229


                  .
gi 1907114272 150 G 150
Cdd:cd01998   230 G 230
MnmA COG0482
tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain [Translation, ...
 71-150 1.80e-27

tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain [Translation, ribosomal structure and biogenesis]; tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440250 [Multi-domain]  Cd Length: 353  Bit Score: 107.06  E-value: 1.80e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907114272  71 TVSQDALRRTIFPLGELTKDFVKKIAAENSLHhVLQKRESMGICFIGKRNLEHFLLQYLQPRPGKFVSIeDNTVLGTHKG 150
Cdd:COG0482   153 RLTQEQLSKTLFPLGELTKPEVREIAEELGLP-VADKKDSQGICFIGDGDYRDFLERYLPEKPGDIVDL-DGKVLGEHDG 230
mnmA PRK00143
tRNA-specific 2-thiouridylase MnmA; Reviewed
 71-150 9.95e-27

tRNA-specific 2-thiouridylase MnmA; Reviewed


Pssm-ID: 234664 [Multi-domain]  Cd Length: 346  Bit Score: 105.15  E-value: 9.95e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907114272  71 TVSQDALRRTIFPLGELTKDFVKKIAAENSLhHVLQKRESMGICFIGKRNLEHFLLQYLQPRPGKFVSIeDNTVLGTHKG 150
Cdd:PRK00143  150 QLTQEQLAKLLFPLGELTKPEVREIAEEAGL-PVAKKKDSQGICFIGERDYRDFLKRYLPAQPGEIVDL-DGKVLGEHKG 227
tRNA_Me_trans pfam03054
tRNA methyl transferase HUP domain; This family represents the N-terminal HUP domain in tRNA ...
 71-118 1.70e-17

tRNA methyl transferase HUP domain; This family represents the N-terminal HUP domain in tRNA(5-methylaminomethyl-2-thiouridine)-methyltransferase which is involved in the biosynthesis of the modified nucleoside 5-methylaminomethyl-2-thiouridine present in the wobble position of some tRNAs.


Pssm-ID: 460787 [Multi-domain]  Cd Length: 202  Bit Score: 77.68  E-value: 1.70e-17
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 1907114272  71 TVSQDALRRTIFPLGELTKDFVKKIAAENSLhHVLQKRESMGICFIGK 118
Cdd:pfam03054 156 TLSQEQLEKLLFPLGELTKEEVRKIAKEAGL-ATAKKKDSQGICFIGK 202
 
Name Accession Description Interval E-value
MnmA_TRMU-like cd01998
MnmA/TRMU family 2-thiouridylases and similar proteins; This family is composed of bacterial ...
 71-150 7.42e-30

MnmA/TRMU family 2-thiouridylases and similar proteins; This family is composed of bacterial tRNA-specific 2-thiouridylase MnmA (EC 2.8.1.13) and mitochondrial tRNA-specific 2-thiouridylase 1 (TRMU or MTU1, EC 2.8.1.14). MnmA catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. TRMU/MTU1 catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln); this is required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. This family belongs to the adenine nucleotide alpha hydrolase (AANH) superfamily that also includes other N-type ATP PPases and ATP sulfurylases. It forms an alpha/beta/alpha fold which binds to the adenosine group.


Pssm-ID: 467502 [Multi-domain]  Cd Length: 349  Bit Score: 113.75  E-value: 7.42e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907114272  71 TVSQDALRRTIFPLGELTKDFVKKIAAENSLhHVLQKRESMGICFIGKRNLEHFLLQYLQPR-PGKFVSIeDNTVLGTHK 149
Cdd:cd01998   152 RLSQEQLSRTLFPLGHLTKSEVREIAREAGL-PVAEKKDSQGICFIGKRDFRDFLKEYLPEKlPGPIVDI-DGKVLGEHK 229


                  .
gi 1907114272 150 G 150
Cdd:cd01998   230 G 230
MnmA COG0482
tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain [Translation, ...
 71-150 1.80e-27

tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain [Translation, ribosomal structure and biogenesis]; tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440250 [Multi-domain]  Cd Length: 353  Bit Score: 107.06  E-value: 1.80e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907114272  71 TVSQDALRRTIFPLGELTKDFVKKIAAENSLHhVLQKRESMGICFIGKRNLEHFLLQYLQPRPGKFVSIeDNTVLGTHKG 150
Cdd:COG0482   153 RLTQEQLSKTLFPLGELTKPEVREIAEELGLP-VADKKDSQGICFIGDGDYRDFLERYLPEKPGDIVDL-DGKVLGEHDG 230
mnmA PRK00143
tRNA-specific 2-thiouridylase MnmA; Reviewed
 71-150 9.95e-27

tRNA-specific 2-thiouridylase MnmA; Reviewed


Pssm-ID: 234664 [Multi-domain]  Cd Length: 346  Bit Score: 105.15  E-value: 9.95e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907114272  71 TVSQDALRRTIFPLGELTKDFVKKIAAENSLhHVLQKRESMGICFIGKRNLEHFLLQYLQPRPGKFVSIeDNTVLGTHKG 150
Cdd:PRK00143  150 QLTQEQLAKLLFPLGELTKPEVREIAEEAGL-PVAKKKDSQGICFIGERDYRDFLKRYLPAQPGEIVDL-DGKVLGEHKG 227
tRNA_Me_trans pfam03054
tRNA methyl transferase HUP domain; This family represents the N-terminal HUP domain in tRNA ...
 71-118 1.70e-17

tRNA methyl transferase HUP domain; This family represents the N-terminal HUP domain in tRNA(5-methylaminomethyl-2-thiouridine)-methyltransferase which is involved in the biosynthesis of the modified nucleoside 5-methylaminomethyl-2-thiouridine present in the wobble position of some tRNAs.


Pssm-ID: 460787 [Multi-domain]  Cd Length: 202  Bit Score: 77.68  E-value: 1.70e-17
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 1907114272  71 TVSQDALRRTIFPLGELTKDFVKKIAAENSLhHVLQKRESMGICFIGK 118
Cdd:pfam03054 156 TLSQEQLEKLLFPLGELTKEEVRKIAKEAGL-ATAKKKDSQGICFIGK 202
mnmA PRK14665
tRNA-specific 2-thiouridylase MnmA; Provisional
 74-152 1.60e-08

tRNA-specific 2-thiouridylase MnmA; Provisional


Pssm-ID: 173128 [Multi-domain]  Cd Length: 360  Bit Score: 54.17  E-value: 1.60e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907114272  74 QDALRRTIFPLGELTKDFVKKIAAENSLHHVLQKRESMGICF--IGKRNLEHFLL----------QYLQPRPGKFVSiED 141
Cdd:PRK14665  153 QEILQRMLLPMGGMTKSEARAYAAERGFEKVAKKRDSLGVCFcpMDYRSFLKKCLcdesgdknrnIYRKVERGRFLD-ES 231

                          90
                  ....*....|.
gi 1907114272 142 NTVLGTHKGSP 152
Cdd:PRK14665  232 GNFIAWHEGYP 242
PRK14664 PRK14664
tRNA-specific 2-thiouridylase MnmA; Provisional
 74-152 3.39e-08

tRNA-specific 2-thiouridylase MnmA; Provisional


Pssm-ID: 173127 [Multi-domain]  Cd Length: 362  Bit Score: 53.03  E-value: 3.39e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907114272  74 QDALRRTIFPLGELTKDFVKKIAAENSLHHVLQKRESMGICFIgKRNLEHFLLQY-----LQPRPGKFVSIEdNTVLGTH 148
Cdd:PRK14664  148 QDILRRCIFPLGNYTKQTVREYLREKGYEAKSKEGESMEVCFI-KGDYRDFLREQcpeldTEVGPGWFVNSE-GVKLGQH 225


                  ....
gi 1907114272 149 KGSP 152
Cdd:PRK14664  226 KGFP 229
tRNA_Me_trans_M pfam20259
tRNA methyl transferase PRC-barrel domain; This family represents a central PRC-barrel domain ...
 122-150 1.40e-06

tRNA methyl transferase PRC-barrel domain; This family represents a central PRC-barrel domain in tRNA(5-methylaminomethyl-2-thiouridine)-methyltransferase which is involved in the biosynthesis of the modified nucleoside 5-methylaminomethyl-2-thiouridine present in the wobble position of some tRNAs.


Pssm-ID: 466410 [Multi-domain]  Cd Length: 66  Bit Score: 44.52  E-value: 1.40e-06
                          10        20
                  ....*....|....*....|....*....
gi 1907114272 122 EHFLLQYLQPRPGKFVSIEDNTVLGTHKG 150
Cdd:pfam20259   1 KDFLKEYLPVKPGDIIDIDTGEVLGEHEG 29
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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