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Conserved domains on  [gi|1590290921|ref|XP_028169574|]
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LOW QUALITY PROTEIN: biotin--protein ligase [Ostrinia furnacalis]

Protein Classification

biotin--[acetyl-CoA-carboxylase] ligase( domain architecture ID 11417403)

biotin--[acetyl-CoA-carboxylase] ligase catalyzes the formation of biotinyl-5'-AMP from biotin and ATP, and the succeeding biotinylation of the biotin carboxyl carrier protein

CATH:  3.30.930.10
EC:  6.3.4.-
Gene Ontology:  GO:0036211|GO:0005524|GO:0009374|GO:0042803
PubMed:  10470036

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 716-949 2.09e-39

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


:

Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 146.47  E-value: 2.09e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 716 VVYADVLSST----LNVTGTPLAHGVACVARRQTAGRGRTGNAWLTPARGQAAISVqVWQkvtPSLPLVQ-----HASAL 786
Cdd:COG0340     2 IEVFDEVDSTndeaKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSL-LLR---PDLPPARlpllsLAAGL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 787 AVVRALRsqpAYEHLEIRIKWPNDIYY-GRevKIGGTATEANCTYNDV--VInIGTGVNITNSqptmcvnDIIAEHNKRH 863
Cdd:COG0340    78 AVAEALR---ELTGVDVGLKWPNDILLnGK--KLAGILIEASGEGDGIdwVV-IGIGINVNQP-------PFDPEELDQP 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 864 GTNLA-----PISVERFLARYCSELERTLEyMASDGGVQAFLEQYYQYWLHTGEEIRVllKDSGTPVAGCVAGVDEAGWL 938
Cdd:COG0340   145 ATSLKeetgkEVDREELLAALLEELEELYD-RFLEEGFAPILEEWRARLATLGRRVRV--ETGGETLEGIAVGIDEDGAL 221

                         250
                  ....*....|.
gi 1590290921 939 LVATPTGELRV 949
Cdd:COG0340   222 LLETADGEIRA 232
 
Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 716-949 2.09e-39

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 146.47  E-value: 2.09e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 716 VVYADVLSST----LNVTGTPLAHGVACVARRQTAGRGRTGNAWLTPARGQAAISVqVWQkvtPSLPLVQ-----HASAL 786
Cdd:COG0340     2 IEVFDEVDSTndeaKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSL-LLR---PDLPPARlpllsLAAGL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 787 AVVRALRsqpAYEHLEIRIKWPNDIYY-GRevKIGGTATEANCTYNDV--VInIGTGVNITNSqptmcvnDIIAEHNKRH 863
Cdd:COG0340    78 AVAEALR---ELTGVDVGLKWPNDILLnGK--KLAGILIEASGEGDGIdwVV-IGIGINVNQP-------PFDPEELDQP 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 864 GTNLA-----PISVERFLARYCSELERTLEyMASDGGVQAFLEQYYQYWLHTGEEIRVllKDSGTPVAGCVAGVDEAGWL 938
Cdd:COG0340   145 ATSLKeetgkEVDREELLAALLEELEELYD-RFLEEGFAPILEEWRARLATLGRRVRV--ETGGETLEGIAVGIDEDGAL 221

                         250
                  ....*....|.
gi 1590290921 939 LVATPTGELRV 949
Cdd:COG0340   222 LLETADGEIRA 232
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 716-889 3.50e-26

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 106.19  E-value: 3.50e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 716 VVYADVLSST------LNVTGTPlaHGVACVARRQTAGRGRTGNAWLTPARGQAAISVqVWQ-KVTPS-LPLVQHASALA 787
Cdd:cd16442     2 LIVLDEIDSTndeakeLARSGAP--EGTVVVAEEQTAGRGRRGRKWESPKGKGLYFSL-LLRpDVPPAeAPLLTLLAAVA 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 788 VVRALRSqpaYEHLEIRIKWPNDIYY-GRevKIGGTATE--ANCTYNDVVInIGTGVNITNSQPTMCVNDIIAEHNKRHG 864
Cdd:cd16442    79 VAEALEK---LGGIPVQIKWPNDILVnGK--KLAGILTEasAEGEGVAAVV-IGIGINVNNTPPPEPLPDTSLATSLGKE 152

                         170       180
                  ....*....|....*....|....*
gi 1590290921 865 TNlAPISVERFLArycsELERTLEY 889
Cdd:cd16442   153 VD-RNELLEELLA----ALENRLEL 172
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 716-940 1.32e-24

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 103.64  E-value: 1.32e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 716 VVYADVLSSTLNVT---GTPLAH-GVACVARRQTAGRGRTGNAWLTPargQAAISVQVW------QKVTPSLPLVqhaSA 785
Cdd:TIGR00121   2 VIVLDVIDSTNQYAlelAKEGKLkGDLVVAEYQTAGRGRRGRKWLSP---EGGLYFSLIlrpdlpKSPAPGLTLV---AG 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 786 LAVVRALRSqpayEHLEIRIKWPNDIYYGrEVKIGGTATEANCTYNDV--VInIGTGVNITNSQPTMCVNDI-IAEhnkr 862
Cdd:TIGR00121  76 IAIAEVLKE----LGDQVQVKWPNDILLK-DKKLGGILTELTGKENRAdyVV-IGIGINVQNRKPAESLREQaISL---- 145

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 863 hgTNLAPISVER--FLARYCSELERTLEyMASDGGVQAFLEQYYQYWLHTGEEIRVlLKDSGTpVAGCVAGVDEAGWLLV 940
Cdd:TIGR00121 146 --SEEAGIDLDRgeLIEGFLRNFEENLE-WFEQEGIDEILSKWEKLSAHIGREVSL-TTGNGE-IEGIARGIDKDGALLL 220
PRK11886 PRK11886
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;
716-942 2.28e-22

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;


Pssm-ID: 237010 [Multi-domain]  Cd Length: 319  Bit Score: 99.09  E-value: 2.28e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 716 VVYADVLSSTlNVTG----TPLAHGVACVARRQTAGRGRTGNAWLTPARGQAAISVqVWQkvtPSLPLVQHAS-----AL 786
Cdd:PRK11886   80 VTVLPVIDST-NQYLldriAELKSGDLCLAEYQTAGRGRRGRQWFSPFGGNLYLSL-YWR---LNQGPAQAMGlslvvGI 154

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 787 AVVRALRSQPAYehlEIRIKWPNDIYYGrEVKIGGTATEANCTYND---VVINIGTGVNITNSQptmcvndiiAEHNKRH 863
Cdd:PRK11886  155 AIAEALRRLGAI---DVGLKWPNDIYLN-DRKLAGILVELSGETGDaahVVIGIGINVAMPDFP---------EELIDQP 221

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 864 GTNL--APISVER--FLARYCSELERTLEYMASDgGVQAFLEQYYQYWLHTGEEIRVLLKDsgTPVAGCVAGVDEAGWLL 939
Cdd:PRK11886  222 WSDLqeAGPTIDRnqLAAELIKQLRAALELFEQE-GLAPFLERWKKLDLFLGREVKLIIGD--KEISGIARGIDEQGALL 298


                  ...
gi 1590290921 940 VAT 942
Cdd:PRK11886  299 LED 301
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 739-842 6.65e-14

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 69.39  E-value: 6.65e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 739 CVARRQTAGRGRTGNAWLTPARGqAAISV-----QVWQKVTPsLPLVQHASALAVVRALRSQPAYEH-LEIRIKWPNDIY 812
Cdd:pfam03099  26 VVVRRQTGGRGRGGNVWHSPKGC-LTYSLllskeHPNVDPSV-LEFYVLELVLAVLEALGLYKPGISgIPCFVKWPNDLY 103

                          90       100       110
                  ....*....|....*....|....*....|
gi 1590290921 813 YGrEVKIGGTATEANCTYNDVVINIGTGVN 842
Cdd:pfam03099 104 VN-GRKLAGILQRSTRGGTLHHGVIGLGVN 132
 
Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 716-949 2.09e-39

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 146.47  E-value: 2.09e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 716 VVYADVLSST----LNVTGTPLAHGVACVARRQTAGRGRTGNAWLTPARGQAAISVqVWQkvtPSLPLVQ-----HASAL 786
Cdd:COG0340     2 IEVFDEVDSTndeaKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSL-LLR---PDLPPARlpllsLAAGL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 787 AVVRALRsqpAYEHLEIRIKWPNDIYY-GRevKIGGTATEANCTYNDV--VInIGTGVNITNSqptmcvnDIIAEHNKRH 863
Cdd:COG0340    78 AVAEALR---ELTGVDVGLKWPNDILLnGK--KLAGILIEASGEGDGIdwVV-IGIGINVNQP-------PFDPEELDQP 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 864 GTNLA-----PISVERFLARYCSELERTLEyMASDGGVQAFLEQYYQYWLHTGEEIRVllKDSGTPVAGCVAGVDEAGWL 938
Cdd:COG0340   145 ATSLKeetgkEVDREELLAALLEELEELYD-RFLEEGFAPILEEWRARLATLGRRVRV--ETGGETLEGIAVGIDEDGAL 221

                         250
                  ....*....|.
gi 1590290921 939 LVATPTGELRV 949
Cdd:COG0340   222 LLETADGEIRA 232
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 716-889 3.50e-26

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 106.19  E-value: 3.50e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 716 VVYADVLSST------LNVTGTPlaHGVACVARRQTAGRGRTGNAWLTPARGQAAISVqVWQ-KVTPS-LPLVQHASALA 787
Cdd:cd16442     2 LIVLDEIDSTndeakeLARSGAP--EGTVVVAEEQTAGRGRRGRKWESPKGKGLYFSL-LLRpDVPPAeAPLLTLLAAVA 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 788 VVRALRSqpaYEHLEIRIKWPNDIYY-GRevKIGGTATE--ANCTYNDVVInIGTGVNITNSQPTMCVNDIIAEHNKRHG 864
Cdd:cd16442    79 VAEALEK---LGGIPVQIKWPNDILVnGK--KLAGILTEasAEGEGVAAVV-IGIGINVNNTPPPEPLPDTSLATSLGKE 152

                         170       180
                  ....*....|....*....|....*
gi 1590290921 865 TNlAPISVERFLArycsELERTLEY 889
Cdd:cd16442   153 VD-RNELLEELLA----ALENRLEL 172
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 716-940 1.32e-24

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 103.64  E-value: 1.32e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 716 VVYADVLSSTLNVT---GTPLAH-GVACVARRQTAGRGRTGNAWLTPargQAAISVQVW------QKVTPSLPLVqhaSA 785
Cdd:TIGR00121   2 VIVLDVIDSTNQYAlelAKEGKLkGDLVVAEYQTAGRGRRGRKWLSP---EGGLYFSLIlrpdlpKSPAPGLTLV---AG 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 786 LAVVRALRSqpayEHLEIRIKWPNDIYYGrEVKIGGTATEANCTYNDV--VInIGTGVNITNSQPTMCVNDI-IAEhnkr 862
Cdd:TIGR00121  76 IAIAEVLKE----LGDQVQVKWPNDILLK-DKKLGGILTELTGKENRAdyVV-IGIGINVQNRKPAESLREQaISL---- 145

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 863 hgTNLAPISVER--FLARYCSELERTLEyMASDGGVQAFLEQYYQYWLHTGEEIRVlLKDSGTpVAGCVAGVDEAGWLLV 940
Cdd:TIGR00121 146 --SEEAGIDLDRgeLIEGFLRNFEENLE-WFEQEGIDEILSKWEKLSAHIGREVSL-TTGNGE-IEGIARGIDKDGALLL 220
PRK11886 PRK11886
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;
716-942 2.28e-22

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;


Pssm-ID: 237010 [Multi-domain]  Cd Length: 319  Bit Score: 99.09  E-value: 2.28e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 716 VVYADVLSSTlNVTG----TPLAHGVACVARRQTAGRGRTGNAWLTPARGQAAISVqVWQkvtPSLPLVQHAS-----AL 786
Cdd:PRK11886   80 VTVLPVIDST-NQYLldriAELKSGDLCLAEYQTAGRGRRGRQWFSPFGGNLYLSL-YWR---LNQGPAQAMGlslvvGI 154

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 787 AVVRALRSQPAYehlEIRIKWPNDIYYGrEVKIGGTATEANCTYND---VVINIGTGVNITNSQptmcvndiiAEHNKRH 863
Cdd:PRK11886  155 AIAEALRRLGAI---DVGLKWPNDIYLN-DRKLAGILVELSGETGDaahVVIGIGINVAMPDFP---------EELIDQP 221

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 864 GTNL--APISVER--FLARYCSELERTLEYMASDgGVQAFLEQYYQYWLHTGEEIRVLLKDsgTPVAGCVAGVDEAGWLL 939
Cdd:PRK11886  222 WSDLqeAGPTIDRnqLAAELIKQLRAALELFEQE-GLAPFLERWKKLDLFLGREVKLIIGD--KEISGIARGIDEQGALL 298


                  ...
gi 1590290921 940 VAT 942
Cdd:PRK11886  299 LED 301
PRK08330 PRK08330
biotin--protein ligase; Provisional
 712-948 1.84e-17

biotin--protein ligase; Provisional


Pssm-ID: 169384 [Multi-domain]  Cd Length: 236  Bit Score: 82.87  E-value: 1.84e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 712 LGRVVVYADVLSSTLNVTGTPLAH---GVACVARRQTAGRGRTGNAWLTPaRGQAAISVQVWQKVTPS-LPLVQHASALA 787
Cdd:PRK08330    1 IGRNIIYFDEVDSTNEYAKRIAPDeeeGTVIVADRQTAGHGRKGRAWASP-EGGLWMSVILKPKVSPEhLPKLVFLGALA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 788 VVRALRSQpayeHLEIRIKWPNDIYYGrEVKIGGTATEANctyNDVVInIGTGVNITNSQPtmcvndiiaEHNKRHGTNL 867
Cdd:PRK08330   80 VVDTLREF----GIEGKIKWPNDVLVN-YKKIAGVLVEGK---GDFVV-LGIGLNVNNEIP---------DELRETATSM 141

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 868 A-----PISVERFLARYCSELER-TLEYMASDGGVqafLEQYYQYWLHTGEEIRVLLkDSGTPVAGCVAGVDEAGWLLVA 941
Cdd:PRK08330  142 KevlgrEVPLIEVFKRLVENLDRwYKLFLEGPGEI---LEEVKGRSMILGKRVKIIG-DGEILVEGIAEDIDEFGALILR 217


                  ....*..
gi 1590290921 942 TPTGELR 948
Cdd:PRK08330  218 LDDGTVK 224
BirA COG1654
Biotin operon repressor [Transcription];
705-949 8.53e-16

Biotin operon repressor [Transcription];


Pssm-ID: 441260 [Multi-domain]  Cd Length: 324  Bit Score: 79.64  E-value: 8.53e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 705 THLTSSQLGRVVVYADVLSSTLNV----TGTPLAHGVACVARRQTAGRGRTGNAWLTPARGqaAISVQVWQKVTPSLPLV 780
Cdd:COG1654    73 AGLSTKRLGREILYVISSTSTNLLalelAAQGGDAGTVVAAEQQRGGRGRRRRSWSSPGGG--GLLYSLLLRPPIAPALL 150

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 781 QHASALAVVRALRSQPAYEHLEIRIKWPNDIYYGREvKIGGTATEANCTYNDVVINIGTGVNITNSQPTMcvNDIIAEHN 860
Cdd:COG1654   151 SLLLLAAAVAVAAALAEGGGLVKWKKWPNDLLKKGK-KILGILEEEGGDADGVVIVVGGGGNNNNSNPEE--EPQELAEL 227

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 861 KRHGTNLAPISVERFLARyCSELERTLEYMASDGGVQAFLEQYYQYWLHTGEEIRVLLKDSGTPVAGCVAGVDEAGWLLV 940
Cdd:COG1654   228 ATSLLLILRLRLLRLLLL-LLLLLLELLELLGFLEFFFLWERLDWELLRVLKLVVVVVEIGGGGGGGGALGGGLLGLLLL 306


                  ....*....
gi 1590290921 941 ATPTGELRV 949
Cdd:COG1654   307 GGGGGGGEG 315
PRK06955 PRK06955
biotin--[acetyl-CoA-carboxylase] ligase;
729-950 8.42e-15

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 235896 [Multi-domain]  Cd Length: 300  Bit Score: 76.36  E-value: 8.42e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 729 TGTPLAHGVACVARRQTAGRGRTGNAWLTPARGQAAISVQVWQKVTPS-LPLVQHASALAVVRALRSQPAYEHLEIRIKW 807
Cdd:PRK06955   58 SADALPAPIVRVAYEQTAGRGRQGRPWFAQPGNALLFSVACVLPRPVAaLAGLSLAVGVALAEALAALPAALGQRIALKW 137

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 808 PNDIYY-GRevKIGGTATE-ANCTYNDVVINIGTGVNITNSQPTMCVNDIIAEHNKRHGTNLAPIS---------VERFL 876
Cdd:PRK06955  138 PNDLLIaGR--KLAGILIEtVWATPDATAVVIGIGLNVRRADAVAAEVDALRAREAALARGLPPVAlaaacaganLTDTL 215

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1590290921 877 ARYCSELERTLEYMASDgGVQAFLEQYYQYWLHTGEEIRvLLKDsGTPVA-GCVAGVDEAGWLLVATPTGELRVA 950
Cdd:PRK06955  216 AAALNALAPALQAFGAD-GLAPFAARWHALHAYAGREVV-LLED-GAELArGVAHGIDETGQLLLDTPAGRQAIA 287
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 739-842 6.65e-14

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 69.39  E-value: 6.65e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 739 CVARRQTAGRGRTGNAWLTPARGqAAISV-----QVWQKVTPsLPLVQHASALAVVRALRSQPAYEH-LEIRIKWPNDIY 812
Cdd:pfam03099  26 VVVRRQTGGRGRGGNVWHSPKGC-LTYSLllskeHPNVDPSV-LEFYVLELVLAVLEALGLYKPGISgIPCFVKWPNDLY 103

                          90       100       110
                  ....*....|....*....|....*....|
gi 1590290921 813 YGrEVKIGGTATEANCTYNDVVINIGTGVN 842
Cdd:pfam03099 104 VN-GRKLAGILQRSTRGGTLHHGVIGLGVN 132
PRK13325 PRK13325
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;
734-949 5.49e-13

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;


Pssm-ID: 183976 [Multi-domain]  Cd Length: 592  Bit Score: 72.82  E-value: 5.49e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 734 AHGVACVARRQTAGRGRTGNAWlTPARGQAAISVQVWQKVTPSLPL--VQHASALAVVRALrsqpAYEHLEIRIKWPNDI 811
Cdd:PRK13325  108 AHKTICVTHLQSKGRGRQGRKW-SHRLGECLMFSFGWVFDRPQYELgsLSPVAAVACRRAL----SRLGLKTQIKWPNDL 182

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 812 YYGREvKIGGTATEANCTYNDVVINIGTGVNITNSQPTMCVNDI--IAEHNKRHGTNLAPISVERFLArycsELERTLEY 889
Cdd:PRK13325  183 VVGRD-KLGGILIETVRTGGKTVAVVGIGINFVLPKEVENAASVqsLFQTASRRGNADAAVLLETLLA----ELDAVLLQ 257

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 890 MASDgGVQAFLEQYYQYWLHTGEEIrVLLKDSGTPVAGCVAGVDEAGWLLVATPTGELRV 949
Cdd:PRK13325  258 YARD-GFAPFVAEYQAANRDHGKAV-LLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTV 315
PTZ00276 PTZ00276
biotin/lipoate protein ligase; Provisional
 722-858 1.33e-11

biotin/lipoate protein ligase; Provisional


Pssm-ID: 140302 [Multi-domain]  Cd Length: 245  Bit Score: 65.66  E-value: 1.33e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 722 LSSTLNVTGTPLAHG----VACVARRQTAGRGRTGNAWLTPaRGQAAISVQVWQK-----VTPSLPLvqhASALAVVRAL 792
Cdd:PTZ00276   15 VTSTMDVARTMLAAAggkpFAVLAESQTAGRGTGGRTWTSP-KGNMYFTLCIPQKgvppeLVPVLPL---ITGLACRAAI 90

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1590290921 793 RSqpAYEHLEIRIKWPNDIYYGREvKIGGTATEANCTYndvvINIGTGVNI------TNS-QPTMCVNDIIAE 858
Cdd:PTZ00276   91 ME--VLHGAAVHTKWPNDIIYAGK-KIGGSLIESEGEY----LIIGIGMNIevappvTDAgRESTMVNEIAED 156
PRK08477 PRK08477
biotin--[acetyl-CoA-carboxylase] ligase;
737-884 2.98e-06

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 236273 [Multi-domain]  Cd Length: 211  Bit Score: 49.18  E-value: 2.98e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590290921 737 VACVARRQTAGRGRTGNAWLTpARGQAAISVQVWQKVTPS-LPLVqhASAL-------AVVRALRSQpayehleIRIKWP 808
Cdd:PRK08477   29 FAIVAKEQTAGIGSRGNSWEG-KKGNLFFSFALKESDLPKdLPLQ--SSSIyfgfllkEVLKELGSK-------VWLKWP 98

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1590290921 809 NDIYYGrEVKIGGTATEancTYNDVVInIGTGVNITNSQPTMCVNDIiaehnkrhgtnlaPISVERFLARYCSELE 884
Cdd:PRK08477   99 NDLYLD-DKKIGGVITN---KIKNFIV-CGIGLNLKFSPKNFACLDI-------------EISDDLLLEGFLQKIE 156
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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