|
Name |
Accession |
Description |
Interval |
E-value |
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
68-666 |
0e+00 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 615.45 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLASRISSALGSdfggLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSEL 147
Cdd:cd05941 1 DRIAIVDDGDSITYADLVARAARLANRLLALGKD----LRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAEL 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 148 EYIISDSQSSLVVaghpyeeilqplaqklglpclplpptnnldaldetdtrekeititdwtdRPAMIIYTSGTTGRPKGV 227
Cdd:cd05941 77 EYVITDSEPSLVL-------------------------------------------------DPALILYTSGTTGRPKGV 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 228 LHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQkvwEMLLSSKAPTINVFMA 307
Cdd:cd05941 108 VLTHANLAANVRALVDAWRWTEDDVLLHVLPLHHVHGLVNALLCPLFAGASVEFLPKFDPK---EVAISRLMPSITVFMG 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 308 VPTIYSKLIQFYDQHYQQPllkEFVKAVCKERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGMALSNPLTGPR 387
Cdd:cd05941 185 VPTIYTRLLQYYEAHFTDP---QFARAAAAERLRLMVSGSAALPVPTLEEWEAITGHTLLERYGMTEIGMALSNPLDGER 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 388 IPGSVGVPLPGVDVRIVMNNKintsivegnh*ntwVRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTgte 467
Cdd:cd05941 262 RPGTVGMPLPGVQARIVDEET--------------GEPLPRGEVGEIQVRGPSVFKEYWNKPEATKEEFTDDGWFKT--- 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 468 pslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahi 547
Cdd:cd05941 --------------------------------------------------------------------------------
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 548 hplGDTAIYK-DGVYWIMGRTSVDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGK-SLTL 625
Cdd:cd05941 325 ---GDLGVVDeDGYYWILGRSSVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRAGAaALSL 401
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 928020699 626 PELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLK 666
Cdd:cd05941 402 EELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
63-668 |
8.37e-115 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 351.81 E-value: 8.37e-115
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 63 APEFGEKLAVIDSCGKHSYKQLYcsslGLASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKH 142
Cdd:COG0318 9 AARHPDRPALVFGGRRLTYAELD----ARARRLAAAL-RALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLNPRL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 143 PPSELEYIISDSQSSLVVAghpyeeilqplaqklglpclplpptnnldaldetdtrekeititdwtdrpAMIIYTSGTTG 222
Cdd:COG0318 84 TAEELAYILEDSGARALVT--------------------------------------------------ALILYTSGTTG 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 223 RPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEMLLSSKaptI 302
Cdd:COG0318 114 RPKGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPLLAGATLVLLPRFDPERVLELIERER---V 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 303 NVFMAVPTIYSKLIQFYDQHyQQPLlkefvkavckERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGMALS-N 381
Cdd:COG0318 191 TVLFGVPTMLARLLRHPEFA-RYDL----------SSLRLVVSGGAPLPPELLERFEERFGVRIVEGYGLTETSPVVTvN 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 382 PL-TGPRIPGSVGVPLPGVDVRIVMnnkintsiVEGnh*ntwvRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFtDDG 460
Cdd:COG0318 260 PEdPGERRPGSVGRPLPGVEVRIVD--------EDG-------RELPPGEVGEIVVRGPNVMKGYWNDPEATAEAF-RDG 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 461 WFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*lts 540
Cdd:COG0318 324 WLRTG------------------------DL------------------------------------------------- 330
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 541 lqrfAHIHPlgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKG 620
Cdd:COG0318 331 ----GRLDE--------DGYLYIVGRKK-DMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVLRPG 397
|
570 580 590 600
....*....|....*....|....*....|....*....|....*...
gi 928020699 621 KSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLKHF 668
Cdd:COG0318 398 AELDAEELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRERY 445
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
65-664 |
4.00e-94 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 298.71 E-value: 4.00e-94
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 65 EFGEKLAVIDSCGKHSYKQLYcsslGLASRISSAL-GSDFGglDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHP 143
Cdd:cd05936 11 RFPDKTALIFMGRKLTYRELD----ALAEAFAAGLqNLGVQ--PGDRVALMLPNCPQFPIAYFGALKAGAVVVPLNPLYT 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 144 PSELEYIISDSQSSLVVAGHPYEEILQPlaqklglpclplpptnnldalDETDTREKEITITDwtdrPAMIIYTSGTTGR 223
Cdd:cd05936 85 PRELEHILNDSGAKALIVAVSFTDLLAA---------------------GAPLGERVALTPED----VAVLQYTSGTTGV 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 224 PKGVLHTHSSIQA-MTQClvsaWAW-----SKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEMLlss 297
Cdd:cd05936 140 PKGAMLTHRNLVAnALQI----KAWledllEGDDVVLAALPLFHVFGLTVALLLPLALGATIVLIPRFRPIGVLKEI--- 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 298 KAPTINVFMAVPTIYSKLIQFydqhyqqpllKEFVKAVcKERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIG- 376
Cdd:cd05936 213 RKHRVTIFPGVPTMYIALLNA----------PEFKKRD-FSSLRLCISGGAPLPVEVAERFEELTGVPIVEGYGLTETSp 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 377 MALSNPLTGPRIPGSVGVPLPGVDVRIVmnnKINTSIVEgnh*ntwvrpglEGKEGELLVRGQSVFKEYWNKPQETRESF 456
Cdd:cd05936 282 VVAVNPLDGPRKPGSIGIPLPGTEVKIV---DDDGEELP------------PGEVGELWVRGPQVMKGYWNRPEETAEAF 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 457 TdDGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsAYcsctirrcrgtlpreercqtsfspmlqtgltptmt 536
Cdd:cd05936 347 V-DGWLRTG------------------------DI--------GY----------------------------------- 358
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 537 *ltslqrfahihplgdtaIYKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQ 616
Cdd:cd05936 359 ------------------MDEDGYFFIVDRKK-DMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFVV 419
|
570 580 590 600
....*....|....*....|....*....|....*....|....*...
gi 928020699 617 LRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05936 420 LKEGASLTEEEIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRREL 467
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
210-659 |
5.36e-81 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 259.91 E-value: 5.36e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 210 RPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIvNKLLCPLWVGATCVMLPDFQAQK 289
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGGL-FGLLGALLAGGTVVLLPKFDPEA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 290 VWEMLlssKAPTINVFMAVPTIYSKLIqfydqhyQQPLLKEFVKAvckeRIRLMVSGSAALPLPTLQRWKEITGHTLLER 369
Cdd:cd04433 80 ALELI---EREKVTILLGVPTLLARLL-------KAPESAGYDLS----SLRALVSGGAPLPPELLERFEEAPGIKLVNG 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 370 YGMTEIG--MALSNPLTGPRIPGSVGVPLPGVDVRIVmnnkintsIVEGnh*ntwvRPGLEGKEGELLVRGQSVFKEYWN 447
Cdd:cd04433 146 YGLTETGgtVATGPPDDDARKPGSVGRPVPGVEVRIV--------DPDG-------GELPPGEIGELVVRGPSVMKGYWN 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 448 KPqETRESFTDDGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsaycsctirrcrgtlpreercqtsfspml 527
Cdd:cd04433 211 NP-EATAAVDEDGWYRTG------------------------DL------------------------------------ 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 528 qtgltptmt*ltslqrfAHIHPlgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATW 607
Cdd:cd04433 230 -----------------GRLDE--------DGYLYIVGRLK-DMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEW 283
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|..
gi 928020699 608 GQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKI 659
Cdd:cd04433 284 GERVVAVVVLRPGADLDAEELRAHVRERLAPYKVPRRVVFVDALPRTASGKI 335
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
67-661 |
1.66e-78 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 256.77 E-value: 1.66e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 67 GEKLAVIDSCGKHSYKQLYcsslGLASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPL-YRKHPPs 145
Cdd:cd17631 9 PDRTALVFGGRSLTYAELD----ERVNRLAHAL-RALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLnFRLTPP- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 146 ELEYIISDSQSSLVVaghpyeeilqplaqklglpclplpptnnldaldetdtrekeititdwtDRPAMIIYTSGTTGRPK 225
Cdd:cd17631 83 EVAYILADSGAKVLF------------------------------------------------DDLALLMYTSGTTGRPK 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 226 GVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEMLlssKAPTINVF 305
Cdd:cd17631 115 GAMLTHRNLLWNAVNALAALDLGPDDVLLVVAPLFHIGGLGVFTLPTLLRGGTVVILRKFDPETVLDLI---ERHRVTSF 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 306 MAVPTIYSKLIqfydqhyQQPLLKEFVKAvckeRIRLMVSGSAALPLPTLQRWKEItGHTLLERYGMTEIGMALSnpLTG 385
Cdd:cd17631 192 FLVPTMIQALL-------QHPRFATTDLS----SLRAVIYGGAPMPERLLRALQAR-GVKFVQGYGMTETSPGVT--FLS 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 386 P----RIPGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFtDDGW 461
Cdd:cd17631 258 PedhrRKLGSAGRPVFFVEVRIVDPDG---------------REVPPGEVGEIVVRGPHVMAGYWNRPEATAAAF-RDGW 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 462 FKTGtepslsspafvlthfsaaDVRTLIDmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltsl 541
Cdd:cd17631 322 FHTG------------------DLGRLDE--------------------------------------------------- 332
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 542 qrfahihplgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGK 621
Cdd:cd17631 333 ----------------DGYLYIVDRKK-DMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVVAVVVPRPGA 395
|
570 580 590 600
....*....|....*....|....*....|....*....|
gi 928020699 622 SLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:cd17631 396 ELDEDELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
96-665 |
3.91e-78 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 256.84 E-value: 3.91e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 96 SSALGSDFGGLDgkRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEeilqplaqk 175
Cdd:PRK07787 35 ATAVAERVAGAR--RVAVLATPTLATVLAVVGALIAGVPVVPVPPDSGVAERRHILADSGAQAWLGPAPDD--------- 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 176 lglpclplpptnnLDALDETDTRekeITITDWT-------DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWS 248
Cdd:PRK07787 104 -------------PAGLPHVPVR---LHARSWHrypepdpDAPALIVYTSGTTGPPKGVVLSRRAIAADLDALAEAWQWT 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 249 KNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEMLlSSKAptiNVFMAVPTIYSKLIQFYDqhyqqpLL 328
Cdd:PRK07787 168 ADDVLVHGLPLFHVHGLVLGVLGPLRIGNRFVHTGRPTPEAYAQAL-SEGG---TLYFGVPTVWSRIAADPE------AA 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 329 KEFVKAvckeriRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGMALSNPLTGPRIPGSVGVPLPGVDVRIVMNNk 408
Cdd:PRK07787 238 RALRGA------RLLVSGSAALPVPVFDRLAALTGHRPVERYGMTETLITLSTRADGERRPGWVGLPLAGVETRLVDED- 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 409 intsiveGNH*ntwvrpGLEGKE-GELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaaDVrt 487
Cdd:PRK07787 311 -------GGPV------PHDGETvGELQVRGPTLFDGYLNRPDATAAAFTADGWFRTG------------------DV-- 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 488 lidmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfAHIHPlgdtaiykDGVYWIMGRT 567
Cdd:PRK07787 358 ---------------------------------------------------------AVVDP--------DGMHRIVGRE 372
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 568 SVDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTlpELQTWAREHMGPYKIPTGLLL 647
Cdd:PRK07787 373 STDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDVAAD--ELIDFVAQQLSVHKRPREVRF 450
|
570
....*....|....*...
gi 928020699 648 VEEMPRNQMGKINKKDLL 665
Cdd:PRK07787 451 VDALPRNAMGKVLKKQLL 468
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
63-664 |
8.64e-77 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 254.73 E-value: 8.64e-77
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 63 APEFGEKLAVIDSCGKHSYKQLYcsslGLASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKH 142
Cdd:PRK06187 16 ARKHPDKEAVYFDGRRTTYAELD----ERVNRLANAL-RALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPINIRL 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 143 PPSELEYIISDSQSSLVVAGHPYEEILQPLA------QKLGLPCLPLPPTNNLDALDETDTREKEITITDWTDR----PA 212
Cdd:PRK06187 91 KPEEIAYILNDAEDRVVLVDSEFVPLLAAILpqlptvRTVIVEGDGPAAPLAPEVGEYEELLAAASDTFDFPDIdendAA 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 213 MIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIIlhtlplhhlhgIV-------NKLLCP---LWVGATCVML 282
Cdd:PRK06187 171 AMLYTSGTTGHPKGVVLSHRNLFLHSLAVCAWLKLSRDDVY-----------LVivpmfhvHAWGLPylaLMAGAKQVIP 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 283 PDFQAQKVWEMLLSSKaPTinVFMAVPTIY-----SKLIQFYDQHyqqpllkefvkavckeRIRLMVSGSAALPLPTLQR 357
Cdd:PRK06187 240 RRFDPENLLDLIETER-VT--FFFAVPTIWqmllkAPRAYFVDFS----------------SLRLVIYGGAALPPALLRE 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 358 WKEITGHTLLERYGMTEIGMALS-NPLT-----GPRIPGSVGVPLPGVDVRIVMNnkintsivEGNH*ntwvrPGLEGKE 431
Cdd:PRK06187 301 FKEKFGIDLVQGYGMTETSPVVSvLPPEdqlpgQWTKRRSAGRPLPGVEARIVDD--------DGDEL-----PPDGGEV 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 432 GELLVRGQSVFKEYWNKPQETRESFtDDGWFKTGtepslsspafvlthfsaaDVrtlidmlpgkgvnsaycsctirrcrg 511
Cdd:PRK06187 368 GEIIVRGPWLMQGYWNRPEATAETI-DGGWLHTG------------------DV-------------------------- 402
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 512 tlpreercqtsfspmlqtgltptmt*ltslqrfAHIHPlgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAH 591
Cdd:PRK06187 403 ---------------------------------GYIDE--------DGYLYITDRIK-DVIISGGENIYPRELEDALYGH 440
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 928020699 592 PDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK06187 441 PAVAEVAVIGVPDEKWGERPVAVVVLKPGATLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVL 513
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
131-664 |
4.17e-76 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 252.49 E-value: 4.17e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 131 SGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQPLAQKLGLPCLPlppTNNLD--------ALDETDTREkei 202
Cdd:PRK07514 76 AGAVFLPLNTAYTLAELDYFIGDAEPALVVCDPANFAWLSKIAAAAGAPHVE---TLDADgtgslleaAAAAPDDFE--- 149
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 203 TITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVML 282
Cdd:PRK07514 150 TVPRGADDLAAILYTSGTTGRSKGAMLSHGNLLSNALTLVDYWRFTPDDVLIHALPIFHTHGLFVATNVALLAGASMIFL 229
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 283 PDFQAQKVWEMLlsskaPTINVFMAVPTIYSKLIQfydqhyqQPllkEFVKAVCKeRIRLMVSGSAALPLPTLQRWKEIT 362
Cdd:PRK07514 230 PKFDPDAVLALM-----PRATVMMGVPTFYTRLLQ-------EP---RLTREAAA-HMRLFISGSAPLLAETHREFQERT 293
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 363 GHTLLERYGMTEIGMALSNPLTGPRIPGSVGVPLPGVDVRIvmnnkinTSIVEGnh*ntwvRPGLEGKEGELLVRGQSVF 442
Cdd:PRK07514 294 GHAILERYGMTETNMNTSNPYDGERRAGTVGFPLPGVSLRV-------TDPETG-------AELPPGEIGMIEVKGPNVF 359
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 443 KEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaaDVrtlidmlpGKgvnsaycsctirrcrgtlpreercqts 522
Cdd:PRK07514 360 KGYWRMPEKTAEEFRADGFFITG------------------DL--------GK--------------------------- 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 523 fspmlqtgltptmt*ltslqrfahihplgdtaIYKDGVYWIMGRtSVDILKSGGFKISALEVECHLLAHPDIADVAVIGA 602
Cdd:PRK07514 387 --------------------------------IDERGYVHIVGR-GKDLIISGGYNVYPKEVEGEIDELPGVVESAVIGV 433
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 928020699 603 PDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK07514 434 PHPDFGEGVTAVVVPKPGAALDEAAILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLL 495
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
59-465 |
4.78e-69 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 231.05 E-value: 4.78e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 59 VFTKAPEFGEKLAV-IDSCGKHSYKQLYCsslgLASRISSALGSdFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVP 137
Cdd:pfam00501 1 LERQAARTPDKTALeVGEGRRLTYRELDE----RANRLAAGLRA-LGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVP 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 138 LYRKHPPSELEYIISDSQSSLVVA-GHPYEEILQPLAQKLGLPCLP-------LPPTNNLDALDETDTREKEITITDWTD 209
Cdd:pfam00501 76 LNPRLPAEELAYILEDSGAKVLITdDALKLEELLEALGKLEVVKLVlvldrdpVLKEEPLPEEAKPADVPPPPPPPPDPD 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 210 RPAMIIYTSGTTGRPKGVLHTHSSIqaMTQCLVSAWAWSKNDIILHTLPLHHL------HGIVNKLLCPLWVGATCVMLP 283
Cdd:pfam00501 156 DLAYIIYTSGTTGKPKGVMLTHRNL--VANVLSIKRVRPRGFGLGPDDRVLSTlplfhdFGLSLGLLGPLLAGATVVLPP 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 284 DFQAQKVWEMLLSSKAPTINVFMAVPTIYSKLIQfydqhyqqpllKEFVKAVCKERIRLMVSGSAALPLPTLQRWKEITG 363
Cdd:pfam00501 234 GFPALDPAALLELIERYKVTVLYGVPTLLNMLLE-----------AGAPKRALLSSLRLVLSGGAPLPPELARRFRELFG 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 364 HTLLERYGMTEIGMALSNPLTGP---RIPGSVGVPLPGVDVRIVmnnkintSIVEGnh*ntwvRPGLEGKEGELLVRGQS 440
Cdd:pfam00501 303 GALVNGYGLTETTGVVTTPLPLDedlRSLGSVGRPLPGTEVKIV-------DDETG-------EPVPPGEPGELCVRGPG 368
|
410 420
....*....|....*....|....*
gi 928020699 441 VFKEYWNKPQETRESFTDDGWFKTG 465
Cdd:pfam00501 369 VMKGYLNDPELTAEAFDEDGWYRTG 393
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
108-666 |
2.81e-66 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 226.04 E-value: 2.81e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 108 GKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVagHPYEEIL--QPLAQKLGL------- 178
Cdd:cd05926 39 GDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVL--TPKGELGpaSRAASKLGLailelal 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 179 ---PCLPLPPTNNLDALDETDTREKEITITDWTDrPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILH 255
Cdd:cd05926 117 dvgVLIRAPSAESLSNLLADKKNAKSEGVPLPDD-LALILHTSGTTGRPKGVPLTHRNLAASATNITNTYKLTPDDRTLV 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 256 TLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEmllSSKAPTINVFMAVPTIYSKLIQFYDQHYQQPLLKefvkav 335
Cdd:cd05926 196 VMPLFHVHGLVASLLSTLAAGGSVVLPPRFSASTFWP---DVRDYNATWYTAVPTIHQILLNRPEPNPESPPPK------ 266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 336 ckerIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIG--MAlSNPL-TGPRIPGSVGVPLpGVDVRIvMNNKints 412
Cdd:cd05926 267 ----LRFIRSCSASLPPAVLEALEATFGAPVLEAYGMTEAAhqMT-SNPLpPGPRKPGSVGKPV-GVEVRI-LDED---- 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 413 iveGNh*ntwvrPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGTEPSLSspafvlthfsaadvrtlidml 492
Cdd:cd05926 336 ---GE-------ILPPGVVGEICLRGPNVTRGYLNNPEANAEAAFKDGWFRTGDLGYLD--------------------- 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 493 pgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiyKDGVYWIMGRTSvDIL 572
Cdd:cd05926 385 ----------------------------------------------------------------ADGYLFLTGRIK-ELI 399
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 573 KSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMP 652
Cdd:cd05926 400 NRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLREGASVTEEELRAFCRKHLAAFKVPKKVYFVDELP 479
|
570
....*....|....
gi 928020699 653 RNQMGKINKKDLLK 666
Cdd:cd05926 480 KTATGKIQRRKVAE 493
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
63-664 |
1.29e-65 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 224.78 E-value: 1.29e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 63 APEFGEKLAVIDSCGKHSYKQLYCSslglASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKH 142
Cdd:PRK07656 15 ARRFGDKEAYVFGDQRLTYAELNAR----VRRAAAAL-AALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRY 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 143 PPSELEYIISDSQSSLVVA-----GHPYEEILQPLAQK-------LGLPCLPLPPTNNLDALDETDTREKEITITDwtDR 210
Cdd:PRK07656 90 TADEAAYILARGDAKALFVlglflGVDYSATTRLPALEhvvicetEEDDPHTEKMKTFTDFLAAGDPAERAPEVDP--DD 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 211 PAMIIYTSGTTGRPKGVLHTHSsiqamtQCLVSAWAWS------KNDIILhtlplhhlhgIVNK----------LLCPLW 274
Cdd:PRK07656 168 VADILFTSGTTGRPKGAMLTHR------QLLSNAADWAeylgltEGDRYL----------AANPffhvfgykagVNAPLM 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 275 VGATCVMLPDFQAQKVWEMLLSSKaptINVFMAVPTIYSKLiqfydqhYQQPLLKEFVKAvckeRIRLMVSGSAALPLPT 354
Cdd:PRK07656 232 RGATILPLPVFDPDEVFRLIETER---ITVLPGPPTMYNSL-------LQHPDRSAEDLS----SLRLAVTGAASMPVAL 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 355 LQRW-KEITGHTLLERYGMTE-IGMALSNPLTGPR--IPGSVGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGK 430
Cdd:PRK07656 298 LERFeSELGVDIVLTGYGLSEaSGVTTFNRLDDDRktVAGTIGTAIAGVENKIV---------------NELGEEVPVGE 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 431 EGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaaDVRTLiDmlpgkgvnsaycsctirrcr 510
Cdd:PRK07656 363 VGELLVRGPNVMKGYYDDPEATAAAIDADGWLHTG------------------DLGRL-D-------------------- 403
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 511 gtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiyKDGVYWIMGRTSvDILKSGGFKISALEVECHLLA 590
Cdd:PRK07656 404 ----------------------------------------------EEGYLYIVDRKK-DMFIVGGFNVYPAEVEEVLYE 436
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 928020699 591 HPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK07656 437 HPAVAEAAVIGVPDERLGEVGKAYVVLKPGAELTEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRAL 510
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
63-659 |
2.24e-64 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 222.68 E-value: 2.24e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 63 APEFGEKLAVI-----DSCGKHSYKQLYcsslGLASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVP 137
Cdd:COG0365 19 AEGRGDKVALIwegedGEERTLTYAELR----REVNRFANAL-RALGVKKGDRVAIYLPNIPEAVIAMLACARIGAVHSP 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 138 LYrkhP---PSELEYIISDSQSSLVVA---------GHPYEEILQPLAQKLGL---------PCLPLPPTNNLDALDETD 196
Cdd:COG0365 94 VF---PgfgAEALADRIEDAEAKVLITadgglrggkVIDLKEKVDEALEELPSlehvivvgrTGADVPMEGDLDWDELLA 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 197 TREKEITITdWTDR--PAMIIYTSGTTGRPKGVLHTHSSI---QAMTQCLV------------SAWAWSKndiilhtlpl 259
Cdd:COG0365 171 AASAEFEPE-PTDAddPLFILYTSGTTGKPKGVVHTHGGYlvhAATTAKYVldlkpgdvfwctADIGWAT---------- 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 260 hhlhGIVNKLLCPLWVGATCVML---PDFQ-AQKVWEMLlsSKAPtINVFMAVPTIYSKLIQFYDqhyqqpllkEFVKAV 335
Cdd:COG0365 240 ----GHSYIVYGPLLNGATVVLYegrPDFPdPGRLWELI--EKYG-VTVFFTAPTAIRALMKAGD---------EPLKKY 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 336 CKERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGMA-LSNPLTGPRIPGSVGVPLPGVDVRIVmNNkintsiv 414
Cdd:COG0365 304 DLSSLRLLGSAGEPLNPEVWEWWYEAVGVPIVDGWGQTETGGIfISNLPGLPVKPGSMGKPVPGYDVAVV-DE------- 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 415 EGNH*ntwVRPgleGKEGELLVRGQ--SVFKEYWNKPQETRESF--TDDGWFKTgtepslsspafvlthfsaadvrtlid 490
Cdd:COG0365 376 DGNP----VPP---GEEGELVIKGPwpGMFRGYWNDPERYRETYfgRFPGWYRT-------------------------- 422
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 491 mlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplGDTAIY-KDGVYWIMGRTSv 569
Cdd:COG0365 423 ------------------------------------------------------------GDGARRdEDGYFWILGRSD- 441
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 570 DILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLT--LP-ELQTWAREHMGPYKIPTGLL 646
Cdd:COG0365 442 DVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQVVKAFVVLKPGVEPSdeLAkELQAHVREELGPYAYPREIE 521
|
650
....*....|...
gi 928020699 647 LVEEMPRNQMGKI 659
Cdd:COG0365 522 FVDELPKTRSGKI 534
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
67-659 |
4.30e-63 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 218.01 E-value: 4.30e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 67 GEKLAVIDSCGKHSYKQLYCSSlglaSRISSALGSdFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSE 146
Cdd:cd05959 18 GDKTAFIDDAGSLTYAELEAEA----RRVAGALRA-LGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLTPDD 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 147 LEYIISDSQSSLVVAGHPYEEILQPLAQKLGLPCLPLPPTNNLDALDETDTREKEI--------TITDWTDRPAMIIYTS 218
Cdd:cd05959 93 YAYYLEDSRARVVVVSGELAPVLAAALTKSEHTLVVLIVSGGAGPEAGALLLAELVaaeaeqlkPAATHADDPAFWLYSS 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 219 GTTGRPKGVLHTHSSIQAMTQCLV-SAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQK-VWEMLLS 296
Cdd:cd05959 173 GSTGRPKGVVHLHADIYWTAELYArNVLGIREDDVCFSAAKLFFAYGLGNSLTFPLSVGATTVLMPERPTPAaVFKRIRR 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 297 SKaPTInvFMAVPTIYSKLIQfyDQHYQQPLLKefvkavckeRIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIG 376
Cdd:cd05959 253 YR-PTV--FFGVPTLYAAMLA--APNLPSRDLS---------SLRLCVSAGEALPAEVGERWKARFGLDILDGIGSTEML 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 377 -MALSNpLTGPRIPGSVGVPLPGVDVRIVmnNKiNTSIVEGnh*ntwvrpgleGKEGELLVRGQSVFKEYWNKPQETRES 455
Cdd:cd05959 319 hIFLSN-RPGRVRYGTTGKPVPGYEVELR--DE-DGGDVAD------------GEPGELYVRGPSSATMYWNNRDKTRDT 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 456 FtDDGWFKTGTEpslsspafvlthfsaadvrtlidmlpgkgvnsaYCsctirrcrgtlpREErcqtsfspmlqtgltptm 535
Cdd:cd05959 383 F-QGEWTRTGDK---------------------------------YV------------RDD------------------ 398
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 536 t*ltslqrfahihplgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVV 615
Cdd:cd05959 399 ----------------------DGFYTYAGRAD-DMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKPKAFV 455
|
570 580 590 600
....*....|....*....|....*....|....*....|....*..
gi 928020699 616 QLRKGKSLT--LP-ELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKI 659
Cdd:cd05959 456 VLRPGYEDSeaLEeELKEFVKDRLAPYKYPRWIVFVDELPKTATGKI 502
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
209-663 |
5.31e-58 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 205.62 E-value: 5.31e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMtqcLVSAWAW-----SKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLP 283
Cdd:PRK05605 219 DDVALILYTSGTTGKPKGAQLTHRNLFAN---AAQGKAWvpglgDGPERVLAALPMFHAYGLTLCLTLAVSIGGELVLLP 295
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 284 DFQAQKVWEmLLSSKAPTinvFM-AVPTIYSKLIqfydqhyqqpllkefvkAVCKER------IRLMVSGSAALPLPTLQ 356
Cdd:PRK05605 296 APDIDLILD-AMKKHPPT---WLpGVPPLYEKIA-----------------EAAEERgvdlsgVRNAFSGAMALPVSTVE 354
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 357 RWKEITGHTLLERYGMTEIG-MALSNPLTGPRIPGSVGVPLPGVDVRIVmnNKINTSIVegnh*ntwvRPglEGKEGELL 435
Cdd:PRK05605 355 LWEKLTGGLLVEGYGLTETSpIIVGNPMSDDRRPGYVGVPFPDTEVRIV--DPEDPDET---------MP--DGEEGELL 421
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 436 VRGQSVFKEYWNKPQETRESFTdDGWFKTGtepslsspafvlthfsaaDVRTLidmlpgkgvnsaycsctirrcrgtlpr 515
Cdd:PRK05605 422 VRGPQVFKGYWNRPEETAKSFL-DGWFRTG------------------DVVVM--------------------------- 455
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 516 EErcqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIA 595
Cdd:PRK05605 456 EE----------------------------------------DGFIRIVDRIK-ELIITGGFNVYPAEVEEVLREHPGVE 494
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 928020699 596 DVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKD 663
Cdd:PRK05605 495 DAAVVGLPREDGSEEVVAAVVLEPGAALDPEGLRAYCREHLTRYKVPRRFYHVDELPRDQLGKVRRRE 562
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
80-660 |
3.60e-54 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 193.20 E-value: 3.60e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLycssLGLASRISSALGSDfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLV 159
Cdd:cd05911 12 TYAQL----RTLSRRLAAGLRKL-GLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVI 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 160 -VAGHPYEEILQPLAQKLGLPCLPLPPTNNLDALDETDTREKEITITDW---------TDRPAMIIYTSGTTGRPKGVLH 229
Cdd:cd05911 87 fTDPDGLEKVKEAAKELGPKDKIIVLDDKPDGVLSIEDLLSPTLGEEDEdlppplkdgKDDTAAILYSSGTTGLPKGVCL 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 230 THSSIQAMT--QCLVSAWAWSKNDIILHTLPLHHLHGIvNKLLCPLWVGATCVMLPDF---------QAQKVWEMLLssk 298
Cdd:cd05911 167 SHRNLIANLsqVQTFLYGNDGSNDVILGFLPLYHIYGL-FTTLASLLNGATVIIMPKFdselfldliEKYKITFLYL--- 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 299 APTINVFMAvptiYSKLIQFYDqhyqqplLKEfvkavckerIRLMVSGSAALplptLQRWKE-----ITGHTLLERYGMT 373
Cdd:cd05911 243 VPPIAAALA----KSPLLDKYD-------LSS---------LRVILSGGAPL----SKELQEllakrFPNATIKQGYGMT 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 374 EIGMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnKINTSIVEGnh*ntwvrpglEGKEGELLVRGQSVFKEYWNKPQETR 453
Cdd:cd05911 299 ETGGILTVNPDGDDKPGSVGRLLPNVEAKIV---DDDGKDSLG-----------PNEPGEICVRGPQVMKGYYNNPEATK 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 454 ESFTDDGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsAYCsctirrcrgtlpreercqtsfspmlqtgltp 533
Cdd:cd05911 365 ETFDEDGWLHTG------------------------DI--------GYF------------------------------- 381
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 534 tmt*ltslqrfahihplgDtaiyKDGVYWIMGRtSVDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTA 613
Cdd:cd05911 382 ------------------D----EDGYLYIVDR-KKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRA 438
|
570 580 590 600
....*....|....*....|....*....|....*....|....*...
gi 928020699 614 VVQLRKGKSLTLPELQTWAREHMGPYK-IPTGLLLVEEMPRNQMGKIN 660
Cdd:cd05911 439 YVVRKPGEKLTEKEVKDYVAKKVASYKqLRGGVVFVDEIPKSASGKIL 486
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
16-465 |
6.78e-54 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 194.93 E-value: 6.78e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 16 TSRWKATPYRTLFysrqRLLEATVRRNSHRSALApswvekqkpvftkAPEFGEKLAVidscgkhSYKQLY----CSSLGL 91
Cdd:COG1022 2 SEFSDVPPADTLP----DLLRRRAARFPDRVALR-------------EKEDGIWQSL-------TWAEFAervrALAAGL 57
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 92 ASRissalgsdfgGLD-GKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHP--YEEI 168
Cdd:COG1022 58 LAL----------GVKpGDRVAILSDNRPEWVIADLAILAAGAVTVPIYPTSSAEEVAYILNDSGAKVLFVEDQeqLDKL 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 169 LQPLAQKLG---------LPCLPLPPTNNLDAL----------DETDTREKEITITDwtdrPAMIIYTSGTTGRPKGVLH 229
Cdd:COG1022 128 LEVRDELPSlrhivvldpRGLRDDPRLLSLDELlalgrevadpAELEARRAAVKPDD----LATIIYTSGTTGRPKGVML 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 230 THSSIQAMTQCLVSAWAWSKNDIIlhtlplhhlhgivnklLC--PLW-------------VGATCVMLPDFQaqKVWEML 294
Cdd:COG1022 204 THRNLLSNARALLERLPLGPGDRT----------------LSflPLAhvfertvsyyalaAGATVAFAESPD--TLAEDL 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 295 LSSKaPTinVFMAVP----TIYSKLIQ------------F---------YDQHYQQ----PLLKEFVKAVC--------K 337
Cdd:COG1022 266 REVK-PT--FMLAVPrvweKVYAGIQAkaeeagglkrklFrwalavgrrYARARLAgkspSLLLRLKHALAdklvfsklR 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 338 E----RIRLMVSGSAALPlPTLQRWKEITGHTLLERYGMTEI-GMALSNPLTGPRIpGSVGVPLPGVDVRIvmnnkints 412
Cdd:COG1022 343 EalggRLRFAVSGGAALG-PELARFFRALGIPVLEGYGLTETsPVITVNRPGDNRI-GTVGPPLPGVEVKI--------- 411
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 928020699 413 ivegnh*ntwvrpgleGKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTG 465
Cdd:COG1022 412 ----------------AEDGEILVRGPNVMKGYYKNPEATAEAFDADGWLHTG 448
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
80-664 |
5.92e-53 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 188.07 E-value: 5.92e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLV 159
Cdd:cd05935 3 TYLELLEVVKKLASFLSNK-----GVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVA 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 160 VAGHPYEEIlqplaqklglpclplpptnnldaldetdtrekeititdwtdrpAMIIYTSGTTGRPKGVLHTHSSIQAMTQ 239
Cdd:cd05935 78 VVGSELDDL-------------------------------------------ALIPYTSGTTGLPKGCMHTHFSAAANAL 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 240 CLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDF------QAQKVWEMLLSSKAPT-INVFMAVPTIy 312
Cdd:cd05935 115 QSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMARWdretalELIEKYKVTFWTNIPTmLVDLLATPEF- 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 313 skliqfydqhyqqpllkefvKAVCKERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTE-IGMALSNPLTGPRIPgS 391
Cdd:cd05935 194 --------------------KTRDLSSLKVLTGGGAPMPPAVAEKLLKLTGLRFVEGYGLTEtMSQTHTNPPLRPKLQ-C 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 392 VGVPLPGVDVRIVmnnKINTSIVEGnh*ntwvrpglEGKEGELLVRGQSVFKEYWNKPQETRESFTDDG---WFKTGTep 468
Cdd:cd05935 253 LGIP*FGVDARVI---DIETGRELP-----------PNEVGEIVVRGPQIFKGYWNRPEETEESFIEIKgrrFFRTGD-- 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 469 slsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahih 548
Cdd:cd05935 --------------------------------------------------------------------------------
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 549 pLGdtAIYKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPE- 627
Cdd:cd05935 317 -LG--YMDEEGYFFFVDRVK-RMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYRGKVTEe 392
|
570 580 590
....*....|....*....|....*....|....*...
gi 928020699 628 -LQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05935 393 dIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
80-666 |
3.00e-52 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 186.39 E-value: 3.00e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLycsslglaSRISSALGSDFGGL---DGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQS 156
Cdd:cd05972 2 SFREL--------KRESAKAANVLAKLglrKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGA 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 157 SLVVAghpyeeilqplaqklglpclplpptnnldalDETDtrekeititdwtdrPAMIIYTSGTTGRPKGVLHTHSsiqA 236
Cdd:cd05972 74 KAIVT-------------------------------DAED--------------PALIYFTSGTTGLPKGVLHTHS---Y 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 237 MTQCLVSAWAW---SKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVM--LPDFQAQKVWEMLLSSKaptINVFMAVPTI 311
Cdd:cd05972 106 PLGHIPTAAYWlglRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVyeGPRFDAERILELLERYG---VTSFCGPPTA 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 312 YSKLIQfydqhyqqPLLKEFVKavckERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGMALSNPLTGPRIPGS 391
Cdd:cd05972 183 YRMLIK--------QDLSSYKF----SHLRLVVSAGEPLNPEVIEWWRAATGLPIRDGYGQTETGLTVGNFPDMPVKPGS 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 392 VGVPLPGVDVRIVMNnkintsivEGnh*ntwvRPGLEGKEGELLVRGQ--SVFKEYWNKPQETRESFTDDgWFKTGteps 469
Cdd:cd05972 251 MGRPTPGYDVAIIDD--------DG-------RELPPGEEGDIAIKLPppGLFLGYVGDPEKTEASIRGD-YYLTG---- 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 470 lsspafvlthfsaadvrtliDMlpgkgvnsAYcsctirrcrgtlpREErcqtsfspmlqtgltptmt*ltslqrfahihp 549
Cdd:cd05972 311 --------------------DR--------AY-------------RDE-------------------------------- 317
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 550 lgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKG----KSLTL 625
Cdd:cd05972 318 --------DGYFWFVGRAD-DIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGyepsEELAE 388
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 928020699 626 pELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLK 666
Cdd:cd05972 389 -ELQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
78-664 |
3.21e-51 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 183.26 E-value: 3.21e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 78 KHSYKQLycssLGLASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSS 157
Cdd:cd05934 3 RWTYAEL----LRESARIAAAL-AALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQ 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 158 LVVaghpyeeilqplaqklglpclplpptnnldaldetdtrekeititdwTDrPAMIIYTSGTTGRPKGVLHTHSsiQAM 237
Cdd:cd05934 78 LVV-----------------------------------------------VD-PASILYTSGTTGPPKGVVITHA--NLT 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 238 TQCLVSAW--AWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEMLLSSKAptiNVFMAVPTIYSKL 315
Cdd:cd05934 108 FAGYYSARrfGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVLLPRFSASRFWSDVRRYGA---TVTNYLGAMLSYL 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 316 iqfydqhYQQPllkefvKAVCKERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGMALSNPLTGPRIPGSVGVP 395
Cdd:cd05934 185 -------LAQP------PSPDDRAHRLRAAYGAPNPPELHEEFEERFGVRLLEGYGMTETIVGVIGPRDEPRRPGSIGRP 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 396 LPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVR---GQSVFKEYWNKPQETRESFTdDGWFKTGtepslss 472
Cdd:cd05934 252 APGYEVRIVDDDG---------------QELPAGEPGELVIRglrGWGFFKGYYNMPEATAEAMR-NGWFHTG------- 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 473 pafvlthfsaadvrtlidmlpgkgvNSAYcsctirrcrgtlpREErcqtsfspmlqtgltptmt*ltslqrfahihplgd 552
Cdd:cd05934 309 -------------------------DLGY-------------RDA----------------------------------- 315
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 553 taiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWA 632
Cdd:cd05934 316 -----DGFFYFVDRKK-DMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPEELFAFC 389
|
570 580 590
....*....|....*....|....*....|..
gi 928020699 633 REHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05934 390 EGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQL 421
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
80-659 |
4.11e-51 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 185.93 E-value: 4.11e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYCSSLGLASRISSALGSDfgglDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLV 159
Cdd:PRK08314 37 SYRELLEEAERLAGYLQQECGVR----KGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPMNREEELAHYVTDSGARVA 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 160 VAGHPYEEILQPLAQKLGLPCLPLppTNNLDALDETDtrekEITITDWTDRP---------------------------- 211
Cdd:PRK08314 113 IVGSELAPKVAPAVGNLRLRHVIV--AQYSDYLPAEP----EIAVPAWLRAEpplqalapggvvawkealaaglapppht 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 212 ------AMIIYTSGTTGRPKGVLHTHSSIQAMTqclVSAWAWS---KNDIILHTLPLHHLHGIVNKLLCPLWVGATCVML 282
Cdd:PRK08314 187 agpddlAVLPYTSGTTGVPKGCMHTHRTVMANA---VGSVLWSnstPESVVLAVLPLFHVTGMVHSMNAPIYAGATVVLM 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 283 PdfqaqkVWEMLLSSKAPT---INVFMAVPTIyskLIQFYdqhyQQPLLKEFvkavCKERIRLMVSGSAALPLPTLQRWK 359
Cdd:PRK08314 264 P------RWDREAAARLIEryrVTHWTNIPTM---VVDFL----ASPGLAER----DLSSLRYIGGGGAAMPEAVAERLK 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 360 EITGHTLLERYGMTE-IGMALSNPLTGPRiPGSVGVPLPGVDVRIVmnnKINTSIVEGnh*ntwvrpglEGKEGELLVRG 438
Cdd:PRK08314 327 ELTGLDYVEGYGLTEtMAQTHSNPPDRPK-LQCLGIPTFGVDARVI---DPETLEELP-----------PGEVGEIVVHG 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 439 QSVFKEYWNKPQETRESFTD-DG--WFKTgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpr 515
Cdd:PRK08314 392 PQVFKGYWNRPEATAEAFIEiDGkrFFRT--------------------------------------------------- 420
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 516 eercqtsfspmlqtgltptmt*ltslqrfahihplGDTAIYKDGVYWIMgrtsVDILK----SGGFKISALEVECHLLAH 591
Cdd:PRK08314 421 -----------------------------------GDLGRMDEEGYFFI----TDRLKrminASGFKVWPAEVENLLYKH 461
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 928020699 592 PDIADVAVIGAPDATWGQKVTAVVQLR---KGKSlTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKI 659
Cdd:PRK08314 462 PAIQEACVIATPDPRRGETVKAVVVLRpeaRGKT-TEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKI 531
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
80-661 |
1.95e-49 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 178.73 E-value: 1.95e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYCsslgLASRISSALGSdFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPL---YRKHppsELEYIISDSQS 156
Cdd:cd05903 3 TYSELDT----RADRLAAGLAA-LGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPIlpfFREH---ELAFILRRAKA 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 157 SLVVaghpyeeilqplaqklglpclplpptnnldaldeTDTREKEITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQA 236
Cdd:cd05903 75 KVFV----------------------------------VPERFRQFDPAAMPDAVALLLFTSGTTGEPKGVMHSHNTLSA 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 237 MTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEmLLSSKAPTInvFMAVPTIYSKLI 316
Cdd:cd05903 121 SIRQYAERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIWDPDKALA-LMREHGVTF--MMGATPFLTDLL 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 317 QfydqhyqqpLLKEFVKAVCkeRIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGMALSNPLTGP--RIPGSVGV 394
Cdd:cd05903 198 N---------AVEEAGEPLS--RLRTFVCGGATVPRSLARRAAELLGAKVCSAYGSTECPGAVTSITPAPedRRLYTDGR 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 395 PLPGVDVRIVMNNKINTSivegnh*ntwvrpglEGKEGELLVRGQSVFKEYWNKPQETRESFtDDGWFKTGtepslsspa 474
Cdd:cd05903 267 PLPGVEIKVVDDTGATLA---------------PGVEGELLSRGPSVFLGYLDRPDLTADAA-PEGWFRTG--------- 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 475 fvlthfsaaDVRTLIDmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdta 554
Cdd:cd05903 322 ---------DLARLDE---------------------------------------------------------------- 328
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 555 iykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTW-AR 633
Cdd:cd05903 329 ---DGYLRITGRSK-DIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGALLTFDELVAYlDR 404
|
570 580
....*....|....*....|....*...
gi 928020699 634 EHMGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:cd05903 405 QGVAKQYWPERLVHVDDLPRTPSGKVQK 432
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
90-664 |
5.67e-49 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 179.41 E-value: 5.67e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 90 GLASRISSALG--SDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLyrkHPPSELE---YIISDSQ-SSLVVAGH 163
Cdd:PRK06188 42 QLADRISRYIQafEALGLGTGDAVALLSLNRPEVLMAIGAAQLAGLRRTAL---HPLGSLDdhaYVLEDAGiSTLIVDPA 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 164 PYEEILQPLAQKLGLPC---LPLPPTNNLDALDETDTRE-KEITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQ 239
Cdd:PRK06188 119 PFVERALALLARVPSLKhvlTLGPVPDGVDLLAAAAKFGpAPLVAAALPPDIAGLAYTGGTTGKPKGVMGTHRSIATMAQ 198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 240 CLVSAWAWSKNdiilhtlplhhlhgivNKLLCP--------------LWVGATCVMLPDFQAQKVWEMLLSSKaptINVF 305
Cdd:PRK06188 199 IQLAEWEWPAD----------------PRFLMCtplshaggafflptLLRGGTVIVLAKFDPAEVLRAIEEQR---ITAT 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 306 MAVPT-IYSKLiqfydQHyqqpllkefvkAVCKER----IRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGMALS 380
Cdd:PRK06188 260 FLVPTmIYALL-----DH-----------PDLRTRdlssLETVYYGASPMSPVRLAEAIERFGPIFAQYYGQTEAPMVIT 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 381 ------NPLTGPRIPGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVRGQSVFKEYWNKPQETRE 454
Cdd:PRK06188 324 ylrkrdHDPDDPKRLTSCGRPTPGLRVALLDEDG---------------REVAQGEVGEICVRGPLVMDGYWNRPEETAE 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 455 SFTDdGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsaycsctirrcrgtlPREErcqtsfspmlqtgltpt 534
Cdd:PRK06188 389 AFRD-GWLHTG------------------------DV----------------------ARED----------------- 404
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 535 mt*ltslqrfahihplgdtaiyKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAV 614
Cdd:PRK06188 405 ----------------------EDGFYYIVDRKK-DMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAV 461
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|
gi 928020699 615 VQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK06188 462 VVLRPGAAVDAAELQAHVKERKGSVHAPKQVDFVDSLPLTALGKPDKKAL 511
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
80-664 |
7.45e-49 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 177.29 E-value: 7.45e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLycssLGLASRISSALGSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLV 159
Cdd:cd05958 12 TYRDL----LALANRIANVLVGELGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDKARITVA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 160 VAGHpyeeilqplaqklglpclplpptnNLDALDETdtrekeititdwtdrpAMIIYTSGTTGRPKGVLHTHSSIQAMTQ 239
Cdd:cd05958 88 LCAH------------------------ALTASDDI----------------CILAFTSGTTGAPKATMHFHRDPLASAD 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 240 ClvsawaWSKN-------DIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEmLLSSKAPTinVFMAVPTIY 312
Cdd:cd05958 128 R------YAVNvlrlredDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEEATPDLLLS-AIARYKPT--VLFTAPTAY 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 313 SKLIQFYDqhYQQPLLKEfvkavckerIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTE-IGMALSNPLTGPRiPGS 391
Cdd:cd05958 199 RAMLAHPD--AAGPDLSS---------LRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTEmFHIFISARPGDAR-PGA 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 392 VGVPLPGVDVRIVMNnkintsivEGNh*ntwvrPGLEGKEGELLVRGQSVfkeYWNKPQETRESFTDDGWFKTGTepsls 471
Cdd:cd05958 267 TGKPVPGYEAKVVDD--------EGN-------PVPDGTIGRLAVRGPTG---CRYLADKRQRTYVQGGWNITGD----- 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 472 spafvlTHFSAAdvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplg 551
Cdd:cd05958 324 ------TYSRDP-------------------------------------------------------------------- 329
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 552 dtaiykDGVYWIMGRtSVDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKS---LTLPEL 628
Cdd:cd05958 330 ------DGYFRHQGR-SDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIpgpVLAREL 402
|
570 580 590
....*....|....*....|....*....|....*.
gi 928020699 629 QTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05958 403 QDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFAL 438
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
108-662 |
2.87e-48 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 175.86 E-value: 2.87e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 108 GKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPyeeilqplaqklglpclplpptn 187
Cdd:cd05907 30 GDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFVEDP----------------------- 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 188 nldaldetdtrekeititdwtDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVN 267
Cdd:cd05907 87 ---------------------DDLATIIYTSGTTGRPKGVMLSHRNILSNALALAERLPATEGDRHLSFLPLAHVFERRA 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 268 KLLCPLWVGATCVMLPDfqAQKVWEMlLSSKAPTInvFMAVPTIYSKLIQFYDQHYQQPLLKEFVKAVCKERIRLMVSGS 347
Cdd:cd05907 146 GLYVPLLAGARIYFASS--AETLLDD-LSEVRPTV--FLAVPRVWEKVYAAIKVKAVPGLKRKLFDLAVGGRLRFAASGG 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 348 AALPlPTLQRWKEITGHTLLERYGMTEIGMALS-NPLTGPRIpGSVGVPLPGVDVRIvmnnkintsivegnh*ntwvrpg 426
Cdd:cd05907 221 APLP-AELLHFFRALGIPVYEGYGLTETSAVVTlNPPGDNRI-GTVGKPLPGVEVRI----------------------- 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 427 leGKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaaDVrtlidmlpgkgvnsaycscti 506
Cdd:cd05907 276 --ADDGEILVRGPNVMLGYYKNPEATAEALDADGWLHTG------------------DL--------------------- 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 507 rrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfAHIHPlgdtaiykDGVYWIMGRtSVDILK-SGGFKISALEVE 585
Cdd:cd05907 315 --------------------------------------GEIDE--------DGFLHITGR-KKDLIItSGGKNISPEPIE 347
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 586 CHLLAHPDIADVAVIGApdatwGQK-VTAVVQLRKGKsltlpeLQTWAREHMGPYKIPTGLL---LVEEMPRNQMGKINK 661
Cdd:cd05907 348 NALKASPLISQAVVIGD-----GRPfLVALIVPDPEA------LEAWAEEHGIAYTDVAELAanpAVRAEIEAAVEAANA 416
|
.
gi 928020699 662 K 662
Cdd:cd05907 417 R 417
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
52-664 |
2.02e-47 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 174.66 E-value: 2.02e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 52 WVEKQkpvftkAPEFGEKLAVIDSCGKHSYKQLYcsslGLASRISSALGSDFGGLDGKRISFLCANDSSYVVAQWAVWMS 131
Cdd:PRK06839 7 WIEKR------AYLHPDRIAIITEEEEMTYKQLH----EYVSKVAAYLIYELNVKKGERIAILSQNSLEYIVLLFAIAKV 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 132 GGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQPLAQKLGLPCLPLpptnnLDALDETDTREKEITITDWTDRP 211
Cdd:PRK06839 77 ECIAVPLNIRLTENELIFQLKDSGTTVLFVEKTFQNMALSMQKVSYVQRVIS-----ITSLKEIEDRKIDNFVEKNESAS 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 212 AMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIvNKLLCPLWVGATCVMLPD-FQAQKV 290
Cdd:PRK06839 152 FIICYTSGTTGKPKGAVLTQENMFWNALNNTFAIDLTMHDRSIVLLPLFHIGGI-GLFAFPTLFAGGVIIVPRkFEPTKA 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 291 WEMLLSSKaptINVFMAVPTIYSKLIQFYDqhYQQPLLkefvkavckERIRLMVSGSAALPLPTLQRWKEiTGHTLLERY 370
Cdd:PRK06839 231 LSMIEKHK---VTVVMGVPTIHQALINCSK--FETTNL---------QSVRWFYNGGAPCPEELMREFID-RGFLFGQGF 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 371 GMTE----IGMALSNPLTgpRIPGSVGVPLPGVDVRIVMNNKINTSIvegnh*ntwvrpgleGKEGELLVRGQSVFKEYW 446
Cdd:PRK06839 296 GMTEtsptVFMLSEEDAR--RKVGSIGKPVLFCDYELIDENKNKVEV---------------GEVGELLIRGPNVMKEYW 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 447 NKPQETRESFTdDGWFKTGtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspm 526
Cdd:PRK06839 359 NRPDATEETIQ-DGWLCTG------------------------------------------------------------- 376
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 527 lqtgltptmt*ltSLQRFAhihplgdtaiyKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDAT 606
Cdd:PRK06839 377 -------------DLARVD-----------EDGFVYIVGRKK-EMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVK 431
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*...
gi 928020699 607 WGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK06839 432 WGEIPIAFIVKKSSSVLIEKDVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQL 489
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
66-664 |
3.87e-47 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 174.35 E-value: 3.87e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 66 FGEKLAVIDSCGKHSYKQLYcsslGLASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPS 145
Cdd:PRK08316 24 YPDKTALVFGDRSWTYAELD----AAVNRVAAAL-LDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGE 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 146 ELEYIISDSQSSLVVAghpyEEILQPLAQKLGLPCLPLPP---------------TNNLDALDETDTREKEITITDwtDR 210
Cdd:PRK08316 99 ELAYILDHSGARAFLV----DPALAPTAEAALALLPVDTLilslvlggreapggwLDFADWAEAGSVAEPDVELAD--DD 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 211 PAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKV 290
Cdd:PRK08316 173 LAQILYTSGTESLPKGAMLTHRALIAEYVSCIVAGDMSADDIPLHALPLYHCAQLDVFLGPYLYVGATNVILDAPDPELI 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 291 WEMLLSSKaptINVFMAVPTIYSKLIQF--YDQHYQQPLLKEFVkavckerirlmvsGSAALPLPTLQRwkeitghtLLE 368
Cdd:PRK08316 253 LRTIEAER---ITSFFAPPTVWISLLRHpdFDTRDLSSLRKGYY-------------GASIMPVEVLKE--------LRE 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 369 R---------YGMTEIGmalsnPLT---GP----RIPGSVGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGKEG 432
Cdd:PRK08316 309 RlpglrfyncYGQTEIA-----PLAtvlGPeehlRRPGSAGRPVLNVETRVV---------------DDDGNDVAPGEVG 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 433 ELLVRGQSVFKEYWNKPQETRESFTdDGWFKTGtepslsspafvlthfsaaDVrtlidmlpgkGVnsaycsctirrcrgt 512
Cdd:PRK08316 369 EIVHRSPQLMLGYWDDPEKTAEAFR-GGWFHSG------------------DL----------GV--------------- 404
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 513 lpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaIYKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHP 592
Cdd:PRK08316 405 ------------------------------------------MDEEGYITVVDRKK-DMIKTGGENVASREVEEALYTHP 441
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 928020699 593 DIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK08316 442 AVAEVAVIGLPDPKWIEAVTAVVVPKAGATVTEDELIAHCRARLAGFKVPKRVIFVDELPRNPSGKILKREL 513
|
|
| menE |
TIGR01923 |
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ... |
80-664 |
2.39e-45 |
|
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]
Pssm-ID: 162605 [Multi-domain] Cd Length: 436 Bit Score: 167.24 E-value: 2.39e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYCSSLGLASRISSalgsdFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLV 159
Cdd:TIGR01923 1 TWQDLDCEAAHLAKALKA-----QGIRSGSRVALVGQNSIEMVLLLHACLLLGAEIAMLNTRLTENERTNQLEDLDVQLL 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 160 VAGHPYEEilqplaqklglpclPLPPTNNLDALDETDTREKEITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSI--QAM 237
Cdd:TIGR01923 76 LTDSLLEE--------------KDFQADSLDRIEAAGRYETSLSASFNMDQIATLMFTSGTTGKPKAVPHTFRNHyaSAV 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 238 TQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLcplwVGATCVMLPDFQAQkVWEMLLSSKaptINVFMAVPTIYSKLIQ 317
Cdd:TIGR01923 142 GSKENLGFTEDDNWLLSLPLYHISGLSILFRWL----IEGATLRIVDKFNQ-LLEMIANER---VTHISLVPTQLNRLLD 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 318 fydqhyqqpllkefvKAVCKERIRLMVSGSAALPLPTLQRWKEiTGHTLLERYGMTEIG---MALSNPLTGPRipGSVGV 394
Cdd:TIGR01923 214 ---------------EGGHNENLRKILLGGSAIPAPLIEEAQQ-YGLPIYLSYGMTETCsqvTTATPEMLHAR--PDVGR 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 395 PLPGVDVRIVmnnkintsivegnh*ntwvRPGLEGkEGELLVRGQSVFKEYWNkPQETRESFTDDGWFKTGTEPSLSSPA 474
Cdd:TIGR01923 276 PLAGREIKIK-------------------VDNKEG-HGEIMVKGANLMKGYLY-QGELTPAFEQQGWFNTGDIGELDGEG 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 475 FVLthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdta 554
Cdd:TIGR01923 335 FLY----------------------------------------------------------------------------- 337
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 555 iykdgvywIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTlpELQTWARE 634
Cdd:TIGR01923 338 --------VLGRRD-DLIISGGENIYPEEIETVLYQHPGIQEAVVVPKPDAEWGQVPVAYIVSESDISQA--KLIAYLTE 406
|
570 580 590
....*....|....*....|....*....|
gi 928020699 635 HMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:TIGR01923 407 KLAKYKVPIAFEKLDELPYNASGKILRNQL 436
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
59-664 |
3.21e-45 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 168.57 E-value: 3.21e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 59 VFTKAPEFGEKLAVIDSC-GKH-SYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVwMSGG--- 133
Cdd:cd05904 11 SFLFASAHPSRPALIDAAtGRAlTYAELERRVRRLAAGLAKR-----GGRKGDVVLLLSPNSIEFPVAFLAV-LSLGavv 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 134 -TAVPLYRkhpPSELEYIISDSQSSLVVAGHPYEEILQPLAQKL----GLPCLPLPPTNNLDALDETDTREKEITITDwt 208
Cdd:cd05904 85 tTANPLST---PAEIAKQVKDSGAKLAFTTAELAEKLASLALPVvlldSAEFDSLSFSDLLFEADEAEPPVVVIKQDD-- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 drPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWA--WSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQ 286
Cdd:cd05904 160 --VAALLYSSGTTGRSKGVMLTHRNLIAMVAQFVAGEGsnSDSEDVFLCVLPMFHIYGLSSFALGLLRLGATVVVMPRFD 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 287 AQKVWEMLLSSKaptINVFMAVPTIYSKLIQfydqhyqQPLLKEFVKAvckeRIRLMVSGSAALPLPTLQRWKEITGHT- 365
Cdd:cd05904 238 LEELLAAIERYK---VTHLPVVPPIVLALVK-------SPIVDKYDLS----SLRQIMSGAAPLGKELIEAFRAKFPNVd 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 366 LLERYGMTE---IGMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnKINTSIVEGnh*ntwvrpglEGKEGELLVRGQSVF 442
Cdd:cd05904 304 LGQGYGMTEstgVVAMCFAPEKDRAKYGSVGRLVPNVEAKIV---DPETGESLP-----------PNQTGELWIRGPSIM 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 443 KEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaaDVrtlidmlpgkgvnsAYcsctirrcrgtlpreercqts 522
Cdd:cd05904 370 KGYLNNPEATAATIDKEGWLHTG------------------DL--------------CY--------------------- 396
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 523 fspmlqtgltptmt*ltslqrfahihplgdtaIYKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGA 602
Cdd:cd05904 397 --------------------------------IDEDGYLFIVDRLK-ELIKYKGFQVAPAELEALLLSHPEILDAAVIPY 443
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 928020699 603 PDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05904 444 PDEEAGEVPMAFVVRKPGSSLTEDEIMDFVAKQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
67-661 |
3.62e-45 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 168.48 E-value: 3.62e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 67 GEKLAVIDSCGKHSYKQLYCSSlglaSRISSALGSdFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSE 146
Cdd:TIGR02262 19 GGKTAFIDDISSLSYGELEAQV----RRLAAALRR-LGVKREERVLLLMLDGVDFPIAFLGAIRAGIVPVALNTLLTADD 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 147 LEYIISDSQSSLVVAGHPYEEILQPLAQKL---GLPCLPLPPTNNLDALDE--TDTREKEITITDWTDRPAMIIYTSGTT 221
Cdd:TIGR02262 94 YAYMLEDSRARVVFVSGALLPVIKAALGKSphlEHRVVVGRPEAGEVQLAEllATESEQFKPAATQADDPAFWLYSSGST 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 222 GRPKGVLHTHSSIQAMTQCLV-SAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDF-QAQKVWEMLLSSKa 299
Cdd:TIGR02262 174 GMPKGVVHTHSNPYWTAELYArNTLGIREDDVCFSAAKLFFAYGLGNALTFPMSVGATTVLMGERpTPDAVFDRLRRHQ- 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 300 PTInvFMAVPTIYSKLIQFYDQHYQQPLlkefvkavckeRIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIG-MA 378
Cdd:TIGR02262 253 PTI--FYGVPTLYAAMLADPNLPSEDQV-----------RLRLCTSAGEALPAEVGQRWQARFGVDIVDGIGSTEMLhIF 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 379 LSNpLTGPRIPGSVGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTd 458
Cdd:TIGR02262 320 LSN-LPGDVRYGTSGKPVPGYRLRLV---------------GDGGQDVADGEPGELLISGPSSATMYWNNRAKSRDTFQ- 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 459 DGWFKTGTepslsspafvlthfsaadvrtlidmlpgKGVNSAycsctirrcrgtlpreercqtsfspmlqtgltptmt*l 538
Cdd:TIGR02262 383 GEWTRSGD----------------------------KYVRND-------------------------------------- 396
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 539 tslqrfahihplgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLR 618
Cdd:TIGR02262 397 -------------------DGSYTYAGRTD-DMLKVSGIYVSPFEIESALIQHPAVLEAAVVGVADEDGLIKPKAFVVLR 456
|
570 580 590 600
....*....|....*....|....*....|....*....|...
gi 928020699 619 KGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:TIGR02262 457 PGQTALETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQR 499
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
68-666 |
9.38e-45 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 166.68 E-value: 9.38e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLASRISsALGSDfgglDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSEL 147
Cdd:PRK03640 17 DRTAIEFEEKKVTFMELHEAVVSVAGKLA-ALGVK----KGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREEL 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 148 EYIISDSQSSLVVAGHPYEEILQPLAQKLglpclplpptnnLDALDETDTREKEItITDWT-DRPAMIIYTSGTTGRPKG 226
Cdd:PRK03640 92 LWQLDDAEVKCLITDDDFEAKLIPGISVK------------FAELMNGPKEEAEI-QEEFDlDEVATIMYTSGTTGKPKG 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 227 VL-----HTHSSI-QAMTQCLVSAWAWskndiilhtlplhhlhgivnklLC--PLW-------------VGATCVMLPDF 285
Cdd:PRK03640 159 VIqtygnHWWSAVgSALNLGLTEDDCW----------------------LAavPIFhisglsilmrsviYGMRVVLVEKF 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 286 QAQKVWEMLLSSKAPTINVfmaVPTIYSKliqfydqhyqqpLLKEFVKAVCKERIRLMVSGSAALPLPTLQRWKEiTGHT 365
Cdd:PRK03640 217 DAEKINKLLQTGGVTIISV---VSTMLQR------------LLERLGEGTYPSSFRCMLLGGGPAPKPLLEQCKE-KGIP 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 366 LLERYGMTEIG---MALSNPLTGPRIpGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvrPGLEGKEGELLVRGQSVF 442
Cdd:PRK03640 281 VYQSYGMTETAsqiVTLSPEDALTKL-GSAGKPLFPCELKIEKDGV----------------VVPPFEEGEIVVKGPNVT 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 443 KEYWNKPQETRESFtDDGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsaycsctirrcrGTLpreercqts 522
Cdd:PRK03640 344 KGYLNREDATRETF-QDGWFKTG------------------------DI-------------------GYL--------- 370
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 523 fspmlqtgltptmt*ltslqrfahihplgDTaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGA 602
Cdd:PRK03640 371 -----------------------------DE----EGFLYVLDRRS-DLIISGGENIYPAEIEEVLLSHPGVAEAGVVGV 416
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 928020699 603 PDATWGQKVTAVVqlRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLK 666
Cdd:PRK03640 417 PDDKWGQVPVAFV--VKSGEVTEEELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQ 478
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
108-668 |
9.58e-45 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 168.03 E-value: 9.58e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 108 GKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAghpyEEILQPLAQKLGLPCLPLPPTN 187
Cdd:PRK07786 67 GDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPEIAFLVSDCGAHVVVT----EAALAPVATAVRDIVPLLSTVV 142
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 188 NL------------DALDETDTREKEITITDwtDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILH 255
Cdd:PRK07786 143 VAggssddsvlgyeDLLAEAGPAHAPVDIPN--DSPALIMYTSGTTGRPKGAVLTHANLTGQAMTCLRTNGADINSDVGF 220
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 256 TLPLHHLHGIVNKLLCPLWVGATCVMLP--DFQAQKVWEMLLSSKAPTInvFMaVPTIYskliqfydqhyqqpllkefvK 333
Cdd:PRK07786 221 VGVPLFHIAGIGSMLPGLLLGAPTVIYPlgAFDPGQLLDVLEAEKVTGI--FL-VPAQW--------------------Q 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 334 AVCKE--------RIRLMVSGSAALPlPTLQRWKEIT--GHTLLERYGMTEIgmalsNPLT-------GPRIPGSVGVPL 396
Cdd:PRK07786 278 AVCAEqqarprdlALRVLSWGAAPAS-DTLLRQMAATfpEAQILAAFGQTEM-----SPVTcmllgedAIRKLGSVGKVI 351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 397 PGVDVRIV---MNNkintsivegnh*ntwVRPgleGKEGELLVRGQSVFKEYWNKPQETRESFtDDGWFKTgtepslssp 473
Cdd:PRK07786 352 PTVAARVVdenMND---------------VPV---GEVGEIVYRAPTLMSGYWNNPEATAEAF-AGGWFHS--------- 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 474 afvlthfsaadvrtlidmlpgkgvnsaycsctirrcrGTLPREErcqtsfspmlqtgltptmt*ltslqrfahihplgdt 553
Cdd:PRK07786 404 -------------------------------------GDLVRQD------------------------------------ 410
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 554 aiyKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKG-KSLTLPELQTWA 632
Cdd:PRK07786 411 ---EEGYVWVVDRKK-DMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRNDdAALTLEDLAEFL 486
|
570 580 590
....*....|....*....|....*....|....*.
gi 928020699 633 REHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLKHF 668
Cdd:PRK07786 487 TDRLARYKHPKALEIVDALPRNPAGKVLKTELRERY 522
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
79-664 |
1.06e-44 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 167.42 E-value: 1.06e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 79 HSYKQLYCSslglASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSL 158
Cdd:cd12119 26 YTYAEVAER----ARRLANAL-RRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHTINPRLFPEQIAYIINHAEDRV 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 159 VVAGHPYEEILQPLAQKLGLPCLPLPPTNNLDALDETDTR--------EKEITITDWTD----RPAMIIYTSGTTGRPKG 226
Cdd:cd12119 101 VFVDRDFLPLLEAIAPRLPTVEHVVVMTDDAAMPEPAGVGvlayeellAAESPEYDWPDfdenTAAAICYTSGTTGNPKG 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 227 VLHTHSSI--QAMTQCLVSAWAWSKNDIIlhtlplhhlhgivnkLLC-PL-------------WVGATCVMLPDFQAQKV 290
Cdd:cd12119 181 VVYSHRSLvlHAMAALLTDGLGLSESDVV---------------LPVvPMfhvnawglpyaaaMVGAKLVLPGPYLDPAS 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 291 WEMLLSSKAPTINVfmAVPTIYSKLIQFYDQHYQQPLlkefvkavckeRIRLMVSGSAALPLPTLQRWKEItGHTLLERY 370
Cdd:cd12119 246 LAELIEREGVTFAA--GVPTVWQGLLDHLEANGRDLS-----------SLRRVVIGGSAVPRSLIEAFEER-GVRVIHAW 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 371 GMTEIG-MALSNPLTGPRIPGSV----------GVPLPGVDVRIVmnnkintsivegnh*ntwvrpGLEGKE-------- 431
Cdd:cd12119 312 GMTETSpLGTVARPPSEHSNLSEdeqlalrakqGRPVPGVELRIV---------------------DDDGRElpwdgkav 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 432 GELLVRGQSVFKEYWNKPQETrESFTDDGWFKTGtepslsspafvlthfsaaDVrtlidmlpgkgvnsaycsctirrcrg 511
Cdd:cd12119 371 GELQVRGPWVTKSYYKNDEES-EALTEDGWLRTG------------------DV-------------------------- 405
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 512 tlpreercqtsfspmlqtgltptmt*ltslqrfAHIHPlgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAH 591
Cdd:cd12119 406 ---------------------------------ATIDE--------DGYLTITDRSK-DVIKSGGEWISSVELENAIMAH 443
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 928020699 592 PDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd12119 444 PAVAEAAVIGVPHPKWGERPLAVVVLKEGATVTAEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKAL 516
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
104-664 |
2.20e-44 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 165.75 E-value: 2.20e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 104 GGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVV------AGHPYEEILQPLAQklg 177
Cdd:PRK09088 43 GCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEPRLLLgddavaAGRTDVEDLAAFIA--- 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 178 lpclplpptnNLDALDETDTREKEititdwTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTL 257
Cdd:PRK09088 120 ----------SADALEPADTPSIP------PERVSLILFTSGTSGQPKGVMLSERNLQQTAHNFGVLGRVDAHSSFLCDA 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 258 PLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKV--WemlLSSKAPTINVFMAVPtiyskliQFYDQHYQQPllkEFVKAV 335
Cdd:PRK09088 184 PMFHIIGLITSVRPVLAVGGSILVSNGFEPKRTlgR---LGDPALGITHYFCVP-------QMAQAFRAQP---GFDAAA 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 336 CKeRIRLMVSGSAALPLPTLQRWKEiTGHTLLERYGMTEIGMALSNPLTGPRIP---GSVGVPLPGVDVRIVMNNkints 412
Cdd:PRK09088 251 LR-HLTALFTGGAPHAAEDILGWLD-DGIPMVDGFGMSEAGTVFGMSVDCDVIRakaGAAGIPTPTVQTRVVDDQ----- 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 413 iveGNH*ntwVRPGLEGkegELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaadvrtlidml 492
Cdd:PRK09088 324 ---GND----CPAGVPG---ELLLRGPNLSPGYWRRPQATARAFTGDGWFRTG--------------------------- 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 493 pgkgvnsaycscTIRRCRGtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYWIMGRTSvDIL 572
Cdd:PRK09088 367 ------------DIARRDA----------------------------------------------DGFFWVVDRKK-DMF 387
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 573 KSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMP 652
Cdd:PRK09088 388 ISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAPLDLERIRSHLSTRLAKYKVPKHLRLVDALP 467
|
570
....*....|..
gi 928020699 653 RNQMGKINKKDL 664
Cdd:PRK09088 468 RTASGKLQKARL 479
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
68-664 |
9.02e-43 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 160.39 E-value: 9.02e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSEL 147
Cdd:cd05930 2 DAVAVVDGDQSLTYAELDARANRLARYLRER-----GVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERL 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 148 EYIISDSQSSLVVAGHpyeeilqplaqklglpclplpptnnldaldetdtrekeititdwtDRPAMIIYTSGTTGRPKGV 227
Cdd:cd05930 77 AYILEDSGAKLVLTDP---------------------------------------------DDLAYVIYTSGSTGKPKGV 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 228 LHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNkLLCPLWVGATCVMLPD---FQAQKVWEMLLSSKaptINV 304
Cdd:cd05930 112 MVEHRGLVNLLLWMQEAYPLTPGDRVLQFTSFSFDVSVWE-IFGALLAGATLVVLPEevrKDPEALADLLAEEG---ITV 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 305 FMAVPTIYskliqfydqhyqQPLLKEFVKAVCKeRIRLMVSGSAALPLPTLQRWKEI-TGHTLLERYGMTEI-GMALSNP 382
Cdd:cd05930 188 LHLTPSLL------------RLLLQELELAALP-SLRLVLVGGEALPPDLVRRWRELlPGARLVNLYGPTEAtVDATYYR 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 383 LTGPRIPGS---VGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDD 459
Cdd:cd05930 255 VPPDDEEDGrvpIGRPIPNTRVYVL---------------DENLRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVPN 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 460 GWF------KTGtepslsspafvlthfsaaD-VRTLIDmlpgkgvnsaycsctirrcrGTLpreercqtsfspmlqtglt 532
Cdd:cd05930 320 PFGpgermyRTG------------------DlVRWLPD--------------------GNL------------------- 342
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 533 ptmt*ltslqrfahihplgdtaiykdgVYwiMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVT 612
Cdd:cd05930 343 ---------------------------EF--LGRID-DQVKIRGYRIELGEIEAALLAHPGVREAAVVAREDGDGEKRLV 392
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|..
gi 928020699 613 AVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05930 393 AYVVPDEGGELDEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
78-664 |
1.99e-42 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 158.66 E-value: 1.99e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 78 KHSYKQLYCSSLGLASRISsALGSDfgglDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSS 157
Cdd:cd05912 1 SYTFAELFEEVSRLAEHLA-ALGVR----KGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDVK 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 158 LvvaghpyeeilqplaqklglpclplpptnnldaldetdtrekeititdwtDRPAMIIYTSGTTGRPKGVLHTHSSIQAm 237
Cdd:cd05912 76 L--------------------------------------------------DDIATIMYTSGTTGKPKGVQQTFGNHWW- 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 238 tqclvSAWAWSKNdiilhtlplhhlHGIVNK--LLCPLWV---------------GATCVMLPDFQAQKVWEMLLSSKAP 300
Cdd:cd05912 105 -----SAIGSALN------------LGLTEDdnWLCALPLfhisglsilmrsviyGMTVYLVDKFDAEQVLHLINSGKVT 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 301 TINVfmaVPTIYSKLIQFYDQHYQqpllkefvkavckERIRLMVSGSAALPLPTLQRWKEiTGHTLLERYGMTEIG--MA 378
Cdd:cd05912 168 IISV---VPTMLQRLLEILGEGYP-------------NNLRCILLGGGPAPKPLLEQCKE-KGIPVYQSYGMTETCsqIV 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 379 LSNPLTGPRIPGSVGVPLPGVDVRIVMNNkintsivegnh*ntwvrpGLEGKEGELLVRGQSVFKEYWNKPQETRESFtD 458
Cdd:cd05912 231 TLSPEDALNKIGSAGKPLFPVELKIEDDG------------------QPPYEVGEILLKGPNVTKGYLNRPDATEESF-E 291
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 459 DGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsAYcsctirrcrgtlpreercqtsfspmlqtgltptmt*l 538
Cdd:cd05912 292 NGWFKTG------------------------DI--------GY------------------------------------- 302
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 539 tslqrfahihplgdtaIYKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLR 618
Cdd:cd05912 303 ----------------LDEEGFLYVLDRRS-DLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSE 365
|
570 580 590 600
....*....|....*....|....*....|....*....|....*.
gi 928020699 619 kgKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05912 366 --RPISEEELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHEL 409
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
69-664 |
2.68e-42 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 158.78 E-value: 2.68e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 69 KLAVIDSCGKHSYKQLYCSSLGLASRISsALGSDFGgldgKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELE 148
Cdd:cd05919 1 KTAFYAADRSVTYGQLHDGANRLGSALR-NLGVSSG----DRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYA 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 149 YIISDSQSSLVVAghpyeeilqplaqklglpclplpptnNLDALdetdtrekeititdwtdrpAMIIYTSGTTGRPKGVL 228
Cdd:cd05919 76 YIARDCEARLVVT--------------------------SADDI-------------------AYLLYSSGTTGPPKGVM 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 229 HTHSSIQAMTQCL-VSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDF-QAQKVWEmLLSSKAPTinVFM 306
Cdd:cd05919 111 HAHRDPLLFADAMaREALGLTPGDRVFSSAKMFFGYGLGNSLWFPLAVGASAVLNPGWpTAERVLA-TLARFRPT--VLY 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 307 AVPTIYSKLIQFYDqhYQQPLLKEfvkavckerIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIG-MALSNPLTG 385
Cdd:cd05919 188 GVPTFYANLLDSCA--GSPDALRS---------LRLCVSAGEALPRGLGERWMEHFGGPILDGIGATEVGhIFLSNRPGA 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 386 PRiPGSVGVPLPGVDVRIVMNnkintsivEGNH*ntwVRPgleGKEGELLVRGQSVFKEYWNKPQETRESFTdDGWFKTG 465
Cdd:cd05919 257 WR-LGSTGRPVPGYEIRLVDE--------EGHT----IPP---GEEGDLLVRGPSAAVGYWNNPEKSRATFN-GGWYRTG 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 466 tepslsspafvlthfsaadvrtliDMlpgkgvnsaYCsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfa 545
Cdd:cd05919 320 ------------------------DK---------FC------------------------------------------- 323
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 546 hihplgdtaIYKDGVYWIMGRtSVDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKG---KS 622
Cdd:cd05919 324 ---------RDADGWYTHAGR-ADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPaapQE 393
|
570 580 590 600
....*....|....*....|....*....|....*....|..
gi 928020699 623 LTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05919 394 SLARDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKL 435
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
80-661 |
1.89e-41 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 158.68 E-value: 1.89e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYCsslgLASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPL---YRKHppsELEYIISDSQS 156
Cdd:PRK13295 57 TYRELAA----LVDRVAVGL-ARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVLNPLmpiFRER---ELSFMLKHAES 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 157 SLVVA-----GHPYEEI---LQP-LAQKLGLPCLPLPPTNNLDALDETDTREKEITITDWTDRP-------AMIIYTSGT 220
Cdd:PRK13295 129 KVLVVpktfrGFDHAAMarrLRPeLPALRHVVVVGGDGADSFEALLITPAWEQEPDAPAILARLrpgpddvTQLIYTSGT 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 221 TGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMlpdfqaQKVWEM-----LL 295
Cdd:PRK13295 209 TGEPKGVMHTANTLMANIVPYAERLGLGADDVILMASPMAHQTGFMYGLMMPVMLGATAVL------QDIWDParaaeLI 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 296 SSKAPTinvFMAVPTiyskliqfydqhyqqPLLKEFVKAVcKER------IRLMVSGSAALPLPTLQRWKEITGHTLLER 369
Cdd:PRK13295 283 RTEGVT---FTMAST---------------PFLTDLTRAV-KESgrpvssLRTFLCAGAPIPGALVERARAALGAKIVSA 343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 370 YGMTEIGMALSNPLTGP--RIPGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVRGQSVFKEYWN 447
Cdd:PRK13295 344 WGMTENGAVTLTKLDDPdeRASTTDGCPLPGVEVRVVDADG---------------APLPAGQIGRLQVRGCSNFGGYLK 408
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 448 KPQETRESFtdDGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsaycsctirrcrgtlpreercqtsfspml 527
Cdd:PRK13295 409 RPQLNGTDA--DGWFDTG------------------------DL------------------------------------ 426
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 528 qtgltptmt*ltslqrfAHIHPlgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATW 607
Cdd:PRK13295 427 -----------------ARIDA--------DGYIRISGRSK-DVIIRGGENIPVVEIEALLYRHPAIAQVAIVAYPDERL 480
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*
gi 928020699 608 GQKVTAVVQLRKGKSLTLPELQTWAREH-MGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:PRK13295 481 GERACAFVVPRPGQSLDFEEMVEFLKAQkVAKQYIPERLVVRDALPRTPSGKIQK 535
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
66-661 |
5.39e-41 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 156.31 E-value: 5.39e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 66 FGEKLAVIDSCGKHSYKQLYCSSLGLASRISSALGSDfggldGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPS 145
Cdd:cd12118 17 YPDRTSIVYGDRRYTWRQTYDRCRRLASALAALGISR-----GDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRLDAE 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 146 ELEYIISDSQSSLVVAGHP--YEEILQ-----PLAQKlglpclplpptnnldALDETDTrekeITITdwtdrpamiiYTS 218
Cdd:cd12118 92 EIAFILRHSEAKVLFVDREfeYEDLLAegdpdFEWIP---------------PADEWDP----IALN----------YTS 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 219 GTTGRPKGVLHTH--------SSIQA--MTQCLVSAW--------AWskndiilhtlplhhlhgivnkllCPLW----VG 276
Cdd:cd12118 143 GTTGRPKGVVYHHrgaylnalANILEweMKQHPVYLWtlpmfhcnGW-----------------------CFPWtvaaVG 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 277 ATCVMLPDFQAQKVWEMLLSSKaptINVFMAVPTIYSKLIQfYDQHYQQPLlkefvkavcKERIRLMVSGSAAlPLPTLQ 356
Cdd:cd12118 200 GTNVCLRKVDAKAIYDLIEKHK---VTHFCGAPTVLNMLAN-APPSDARPL---------PHRVHVMTAGAPP-PAAVLA 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 357 RWKEItGHTLLERYGMTEI-GMALSNP-------LTGP---RIPGSVGVPLPGVDVRIVMNNKINTSIvegnh*ntwvrP 425
Cdd:cd12118 266 KMEEL-GFDVTHVYGLTETyGPATVCAwkpewdeLPTEeraRLKARQGVRYVGLEEVDVLDPETMKPV-----------P 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 426 GlEGKE-GELLVRGQSVFKEYWNKPQETRESFtDDGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsaycsc 504
Cdd:cd12118 334 R-DGKTiGEIVFRGNIVMKGYLKNPEATAEAF-RGGWFHSG------------------------DL------------- 374
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 505 tirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfAHIHPlgdtaiykDGVYWIMGRtSVDILKSGGFKISALEV 584
Cdd:cd12118 375 ----------------------------------------AVIHP--------DGYIEIKDR-SKDIIISGGENISSVEV 405
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 928020699 585 ECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGlLLVEEMPRNQMGKINK 661
Cdd:cd12118 406 EGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKVTEEEIIAFCREHLAGFMVPKT-VVFGELPKTSTGKIQK 481
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
108-666 |
6.20e-41 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 157.50 E-value: 6.20e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 108 GKRISFLCANDSSYVVAQWAVWMSGGTAV---PLYRKHppsELEYIISDSQSSLVV-------------AGHPYEEIL-- 169
Cdd:PRK06710 74 GDRVAIMLPNCPQAVIGYYGTLLAGGIVVqtnPLYTER---ELEYQLHDSGAKVILcldlvfprvtnvqSATKIEHVIvt 150
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 170 -----QPLAQKLGLPCLPLPPTNNLDALDETDT------REKEI-----TITDWTDRPAMIIYTSGTTGRPKGVLHTHSS 233
Cdd:PRK06710 151 riadfLPFPKNLLYPFVQKKQSNLVVKVSESETihlwnsVEKEVntgveVPCDPENDLALLQYTGGTTGFPKGVMLTHKN 230
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 234 IQAMTQCLVSaWAWS---KNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEMLLSSKaptINVFMAVPT 310
Cdd:PRK06710 231 LVSNTLMGVQ-WLYNckeGEEVVLGVLPFFHVYGMTAVMNLSIMQGYKMVLIPKFDMKMVFEAIKKHK---VTLFPGAPT 306
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 311 IYSKLIQfydqhyqQPLLKEFVKAvckeRIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIG-MALSNPLTGPRIP 389
Cdd:PRK06710 307 IYIALLN-------SPLLKEYDIS----SIRACISGSAPLPVEVQEKFETVTGGKLVEGYGLTESSpVTHSNFLWEKRVP 375
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 390 GSVGVPLPGVDVRIVmnnkintSIVEGNH*ntwVRPgleGKEGELLVRGQSVFKEYWNKPQETrESFTDDGWFKTGteps 469
Cdd:PRK06710 376 GSIGVPWPDTEAMIM-------SLETGEA----LPP---GEIGEIVVKGPQIMKGYWNKPEET-AAVLQDGWLHTG---- 436
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 470 lsspafvlthfsaaDVRTLIDmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihp 549
Cdd:PRK06710 437 --------------DVGYMDE----------------------------------------------------------- 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 550 lgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQ 629
Cdd:PRK06710 444 --------DGFFYVKDRKK-DMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVVLKEGTECSEEELN 514
|
570 580 590
....*....|....*....|....*....|....*..
gi 928020699 630 TWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLK 666
Cdd:PRK06710 515 QFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLIE 551
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
209-667 |
9.32e-41 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 157.81 E-value: 9.32e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMtqclvsawAWSKNDIILHTLPLHhlhgivnkLLCPL---WVGA--TCVMLP 283
Cdd:PRK07529 213 DDVAAYFHTGGTTGMPKLAQHTHGNEVAN--------AWLGALLLGLGPGDT--------VFCGLplfHVNAllVTGLAP 276
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 284 DFQAQKV-----------------WEMLLSSKaptINVFMAVPTIYSKLIQfydqhyqQPllkefVKAVCKERIRLMVSG 346
Cdd:PRK07529 277 LARGAHVvlatpqgyrgpgvianfWKIVERYR---INFLSGVPTVYAALLQ-------VP-----VDGHDISSLRYALCG 341
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 347 SAALPLPTLQRWKEITGHTLLERYGMTEiGMALS--NPLTGPRIPGSVGVPLPGVDVRIVmnnkintsIVEGNh*NTWVR 424
Cdd:PRK07529 342 AAPLPVEVFRRFEAATGVRIVEGYGLTE-ATCVSsvNPPDGERRIGSVGLRLPYQRVRVV--------ILDDA--GRYLR 410
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 425 PGLEGKEGELLVRGQSVFKEYWNKPQEtRESFTDDGWFKTGtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsc 504
Cdd:PRK07529 411 DCAVDEVGVLCIAGPNVFSGYLEAAHN-KGLWLEDGWLNTG--------------------------------------- 450
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 505 tirrcrgtlpreercqtsfspmlqtgltptmt*ltSLQRFahihplgDTaiykDGVYWIMGRTSvDILKSGGFKISALEV 584
Cdd:PRK07529 451 -----------------------------------DLGRI-------DA----DGYFWLTGRAK-DLIIRGGHNIDPAAI 483
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 585 ECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMG-PYKIPTGLLLVEEMPRNQMGKINKKD 663
Cdd:PRK07529 484 EEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGASATEAELLAFARDHIAeRAAVPKHVRILDALPKTAVGKIFKPA 563
|
....
gi 928020699 664 LLKH 667
Cdd:PRK07529 564 LRRD 567
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
61-666 |
1.06e-39 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 153.75 E-value: 1.06e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 61 TKAPEFGEKLAVIDSCG-KHSYKQLYCsslgLASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLY 139
Cdd:PRK06087 31 QTARAMPDKIAVVDNHGaSYTYSALDH----AASRLANWL-LAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLL 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 140 RKHPPSELEYIISDSQSSLVVAghP-------YEEILQPLAQKLGLPCLPLPPTNNLDA---------LDETDTREKEIT 203
Cdd:PRK06087 106 PSWREAELVWVLNKCQAKMFFA--PtlfkqtrPVDLILPLQNQLPQLQQIVGVDKLAPAtsslslsqiIADYEPLTTAIT 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 204 ITdwTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLP 283
Cdd:PRK06087 184 TH--GDELAAVLFTSGTEGLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLD 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 284 DFQAQKVWEMLLSSKAPTinVFMAVPTIYSKLIQFYDQHYQQPLLkefvkavckeriRLMVSGSAALPLPTLQRWKEiTG 363
Cdd:PRK06087 262 IFTPDACLALLEQQRCTC--MLGATPFIYDLLNLLEKQPADLSAL------------RFFLCGGTTIPKKVARECQQ-RG 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 364 HTLLERYGMTE----IGMALSNPLtgPRIPGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVRGQ 439
Cdd:PRK06087 327 IKLLSVYGSTEssphAVVNLDDPL--SRFMHTDGYAAAGVEIKVVDEAR---------------KTLPPGCEGEEASRGP 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 440 SVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsaycsctirrCRgtlpreerc 519
Cdd:PRK06087 390 NVFMGYLDEPELTARALDEEGWYYSG------------------------DL-----------------CR--------- 419
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 520 qtsfspmlqtgltptmt*ltslqrfahihplgdtaIYKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAV 599
Cdd:PRK06087 420 -----------------------------------MDEAGYIKITGRKK-DIIVRGGENISSREVEDILLQHPKIHDACV 463
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 928020699 600 IGAPDATWGQKVTAVVQLRKG-KSLTLPELQTW-AREHMGPYKIPTGLLLVEEMPRNQMGKINK----KDLLK 666
Cdd:PRK06087 464 VAMPDERLGERSCAYVVLKAPhHSLTLEEVVAFfSRKRVAKYKYPEHIVVIDKLPRTASGKIQKfllrKDIMR 536
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
91-666 |
6.64e-39 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 149.19 E-value: 6.64e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 91 LASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAghpYEEILq 170
Cdd:cd05969 9 LSARFANVL-KSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLIT---TEELY- 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 171 plaqklglpclplpptnnldaldetDTREKEititdwtdRPAMIIYTSGTTGRPKGVLHTHSsiqAMTQCLVSAwAWS-- 248
Cdd:cd05969 84 -------------------------ERTDPE--------DPTLLHYTSGTTGTPKGVLHVHD---AMIFYYFTG-KYVld 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 249 --KNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLP-DFQAQKVWEMLLSSKaptINVFMAVPTIYSKLIQFYDqhyqq 325
Cdd:cd05969 127 lhPDDIYWCTADPGWVTGTVYGIWAPWLNGVTNVVYEgRFDAESWYGIIERVK---VTVWYTAPTAIRMLMKEGD----- 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 326 pllkEFVKAVCKERIRLMVSGSAALPlPTLQRW-KEITGHTLLERYGMTEIG-MALSNPLTGPRIPGSVGVPLPGVDVRI 403
Cdd:cd05969 199 ----ELARKYDLSSLRFIHSVGEPLN-PEAIRWgMEVFGVPIHDTWWQTETGsIMIANYPCMPIKPGSMGKPLPGVKAAV 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 404 VMNNkintsiveGNH*ntwVRPgleGKEGELLVRGQ--SVFKEYWNKPQETRESFTdDGWFKTGtepslsspafvlthfs 481
Cdd:cd05969 274 VDEN--------GNE----LPP---GTKGILALKPGwpSMFRGIWNDEERYKNSFI-DGWYLTG---------------- 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 482 aadvrtlidmlpgkgvNSAYcsctirrcrgtlpREErcqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVY 561
Cdd:cd05969 322 ----------------DLAY-------------RDE----------------------------------------DGYF 332
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 562 WIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLP---ELQTWAREHMGP 638
Cdd:cd05969 333 WFVGRAD-DIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDElkeEIINFVRQKLGA 411
|
570 580
....*....|....*....|....*...
gi 928020699 639 YKIPTGLLLVEEMPRNQMGKINKKdLLK 666
Cdd:cd05969 412 HVAPREIEFVDNLPKTRSGKIMRR-VLK 438
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
91-666 |
3.97e-38 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 149.42 E-value: 3.97e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 91 LASRISSALGSdFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVP---LYRKHppsELEYIISDSQSSLVVA------ 161
Cdd:PRK06178 67 LSDRFAALLRQ-RGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPvspLFREH---ELSYELNDAGAEVLLAldqlap 142
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 162 -------GHPYEEILQ-------------PLAQKLGLPCLPLPPTNNLDALDETDTREKEITITDwTDRPAMIIYTSGTT 221
Cdd:PRK06178 143 vveqvraETSLRHVIVtsladvlpaeptlPLPDSLRAPRLAAAGAIDLLPALRACTAPVPLPPPA-LDALAALNYTGGTT 221
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 222 GRPKGVLHTHSSI--QAMTQCLVSAwAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEmllsska 299
Cdd:PRK06178 222 GMPKGCEHTQRDMvyTAAAAYAVAV-VGGEDSVFLSFLPEFWIAGENFGLLFPLFSGATLVLLARWDAVAFMA------- 293
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 300 ptinvfmAVP----TIYSKLIQFYDQHYQQPLLKEF----VKAVckerirLMVSGSAALPLPTLQRWKEITGHTLLE-RY 370
Cdd:PRK06178 294 -------AVEryrvTRTVMLVDNAVELMDHPRFAEYdlssLRQV------RVVSFVKKLNPDYRQRWRALTGSVLAEaAW 360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 371 GMTE--------IGMA------LSNPLTgpripgsVGVPLPGVDVRIVmnnkintsivegNH*NTWVRPglEGKEGELLV 436
Cdd:PRK06178 361 GMTEthtcdtftAGFQdddfdlLSQPVF-------VGLPVPGTEFKIC------------DFETGELLP--LGAEGEIVV 419
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 437 RGQSVFKEYWNKPQETRESFTDdGWFKTGtepslsspafvlthfsaaDVrtlidmlpGKgvnsaycsctirrcrgtlpre 516
Cdd:PRK06178 420 RTPSLLKGYWNKPEATAEALRD-GWLHTG------------------DI--------GK--------------------- 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 517 ercqtsfspmlqtgltptmt*ltslqrfahihplgdtaIYKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIAD 596
Cdd:PRK06178 452 --------------------------------------IDEQGFLHYLGRRK-EMLKVNGMSVFPSEVEALLGQHPAVLG 492
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 597 VAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTgLLLVEEMPRNQMGKINKKDLLK 666
Cdd:PRK06178 493 SAVVGRPDPDKGQVPVAFVQLKPGADLTAAALQAWCRENMAVYKVPE-IRIVDALPMTATGKVRKQDLQA 561
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
211-661 |
2.77e-37 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 142.02 E-value: 2.77e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 211 PAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIvNKLLCPLWVGATCVMLPDFQAQKV 290
Cdd:cd17637 2 PFVIIHTAAVAGRPRGAVLSHGNLIAANLQLIHAMGLTEADVYLNMLPLFHIAGL-NLALATFHAGGANVVMEKFDPAEA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 291 WEMLLSSKaptINVFMAVPTIYSKLIqfyDQHYQQPLLKEFVKAVckerirlmvsgsAALPLP-TLQRWKEITGHTLLER 369
Cdd:cd17637 81 LELIEEEK---VTLMGSFPPILSNLL---DAAEKSGVDLSSLRHV------------LGLDAPeTIQRFEETTGATFWSL 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 370 YGMTEIgmalSNPLTGPRI---PGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVRGQSVFKEYW 446
Cdd:cd17637 143 YGQTET----SGLVTLSPYrerPGSAGRPGPLVRVRIVDDND---------------RPVPAGETGEIVVRGPLVFQGYW 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 447 NKPQETRESFtDDGWFKTGtepslsspafvlthfsaadvrtliDMlpGKgvnsaycsctirrcrgtlpreercqtsfspm 526
Cdd:cd17637 204 NLPELTAYTF-RNGWHHTG------------------------DL--GR------------------------------- 225
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 527 lqtgltptmt*ltslqrfahihplgdtaIYKDGVYWIMGRTSV-DILKSGGFKISALEVECHLLAHPDIADVAVIGAPDA 605
Cdd:cd17637 226 ----------------------------FDEDGYLWYAGRKPEkELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDP 277
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*.
gi 928020699 606 TWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:cd17637 278 KWGEGIKAVCVLKPGATLTADELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
76-465 |
3.96e-37 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 144.51 E-value: 3.96e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 76 CGKHSYKQL--YCSSLGLASRISsalgsdfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISD 153
Cdd:cd05914 5 GEPLTYKDLadNIAKFALLLKIN-------GVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNH 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 154 SQSSLVVAGhpyeeilqplaqklglpclplpptnnldalDETDTrekeititdwtdrpAMIIYTSGTTGRPKGVLHTHSS 233
Cdd:cd05914 78 SEAKAIFVS------------------------------DEDDV--------------ALINYTSGTTGNSKGVMLTYRN 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 234 IQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVweMLLSSKAPTINVFMAVPTIYS 313
Cdd:cd05914 114 IVSNVDGVKEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDKIPSAKI--IALAFAQVTPTLGVPVPLVIE 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 314 KLIQ-----------FYDQHYQQPLLKEFVKAVCKE-------RIRLMVSGSAALPLPTLQRWKEItGHTLLERYGMTEI 375
Cdd:cd05914 192 KIFKmdiipkltlkkFKFKLAKKINNRKIRKLAFKKvheafggNIKEFVIGGAKINPDVEEFLRTI-GFPYTIGYGMTET 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 376 GMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnkintsivegnh*ntwvRPGLEGKEGELLVRGQSVFKEYWNKPQETRES 455
Cdd:cd05914 271 APIISYSPPNRIRLGSAGKVIDGVEVRID-------------------SPDPATGEGEIIVRGPNVMKGYYKNPEATAEA 331
|
410
....*....|
gi 928020699 456 FTDDGWFKTG 465
Cdd:cd05914 332 FDKDGWFHTG 341
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
78-664 |
5.53e-37 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 143.73 E-value: 5.53e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 78 KHSYKQLYCSSLGLASRISSAlgsdfgGLD-GKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQS 156
Cdd:cd05971 6 KVTFKELKTASNRFANVLKEI------GLEkGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 157 SLVVaghpyeeilqplaqklglpclplpptnnldaldetdtrekeitiTDWTDRPAMIIYTSGTTGRPKGVLHTHS---- 232
Cdd:cd05971 80 SALV--------------------------------------------TDGSDDPALIIYTSGTTGPPKGALHAHRvllg 115
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 233 ---SIQAMTQCL---------VSAWAWskndiilhtlplhhLHGIVNKLLCPLWVGATCV---MLPdFQAQKVWEMLlsS 297
Cdd:cd05971 116 hlpGVQFPFNLFprdgdlywtPADWAW--------------IGGLLDVLLPSLYFGVPVLahrMTK-FDPKAALDLM--S 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 298 KAPTINVFMavPTIYSKLIQFydqhyQQPLLKEFVKavckeRIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGM 377
Cdd:cd05971 179 RYGVTTAFL--PPTALKMMRQ-----QGEQLKHAQV-----KLRAIATGGESLGEELLGWAREQFGVEVNEFYGQTECNL 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 378 ALSN-PLTGPRIPGSVGVPLPGVDVRIVMNNkintsiveGNH*ntwVRPgleGKEGELLVR--GQSVFKEYWNKPQETRE 454
Cdd:cd05971 247 VIGNcSALFPIKPGSMGKPIPGHRVAIVDDN--------GTP----LPP---GEVGEIAVElpDPVAFLGYWNNPSATEK 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 455 SFTDDgWFKTgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltpt 534
Cdd:cd05971 312 KMAGD-WLLT---------------------------------------------------------------------- 320
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 535 mt*ltslqrfahihplGDTAIY-KDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTA 613
Cdd:cd05971 321 ----------------GDLGRKdSDGYFWYVGRDD-DVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKA 383
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....
gi 928020699 614 VVQLRKG--KSLTLP-ELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05971 384 FVVLNPGetPSDALArEIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRREL 437
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
132-664 |
1.73e-36 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 143.67 E-value: 1.73e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 132 GGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQPLAQKLGLPCLPLPPTN-NLDALDET-----------DTRE 199
Cdd:PRK08008 86 GAIMVPINARLLREESAWILQNSQASLLVTSAQFYPMYRQIQQEDATPLRHICLTRvALPADDGVssftqlkaqqpATLC 165
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 200 KEITITdwTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTqcLVSAW--AWSKNDIILHTLPLHHLHGIVNKLLCPLWVGA 277
Cdd:PRK08008 166 YAPPLS--TDDTAEILFTSGTTSRPKGVVITHYNLRFAG--YYSAWqcALRDDDVYLTVMPAFHIDCQCTAAMAAFSAGA 241
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 278 TCVMLPDFQAQKVWEMLLSSKAptiNVFMAVPTIYSKLIQfydqhyqQPLlKEFVKAVCKERIRLMVSGSAALPLPTLQR 357
Cdd:PRK08008 242 TFVLLEKYSARAFWGQVCKYRA---TITECIPMMIRTLMV-------QPP-SANDRQHCLREVMFYLNLSDQEKDAFEER 310
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 358 WkeitGHTLLERYGMTE-IGMALSNPLTGPRIPGSVGVPLPGVDVRIVmnNKINTSIVEGNH*NTWVRPglegkegellV 436
Cdd:PRK08008 311 F----GVRLLTSYGMTEtIVGIIGDRPGDKRRWPSIGRPGFCYEAEIR--DDHNRPLPAGEIGEICIKG----------V 374
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 437 RGQSVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaadvrtlidmlpgkgvNSAYCSctirrcrgtlpre 516
Cdd:PRK08008 375 PGKTIFKEYYLDPKATAKVLEADGWLHTG--------------------------------DTGYVD------------- 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 517 ercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiyKDGVYWIMGRtSVDILKSGGFKISALEVECHLLAHPDIAD 596
Cdd:PRK08008 410 ----------------------------------------EEGFFYFVDR-RCNMIKRGGENVSCVELENIIATHPKIQD 448
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 928020699 597 VAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK08008 449 IVVVGIKDSIRDEAIKAFVVLNEGETLSEEEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNL 516
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
214-661 |
3.94e-36 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 138.79 E-value: 3.94e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 214 IIYTSGTTGRPKGVLHTHSsiqamtQCLVSAWAWSKNDIILHTLPLHhlhgIVNKL----------LCPLWVGATCVMLP 283
Cdd:cd17638 5 IMFTSGTTGRSKGVMCAHR------QTLRAAAAWADCADLTEDDRYL----IINPFfhtfgykagiVACLLTGATVVPVA 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 284 DFQAQKVWEMLLSSKaptINVFMAVPTIYSKLIQfydqhyqQPLLKEFVKAvckeRIRLMVSGSAALPLPTLQRWKEITG 363
Cdd:cd17638 75 VFDVDAILEAIERER---ITVLPGPPTLFQSLLD-------HPGRKKFDLS----SLRAAVTGAATVPVELVRRMRSELG 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 364 -HTLLERYGMTEIGMA-LSNPLTGPR-IPGSVGVPLPGVDVRIvmnnkintsivegnh*ntwvrpgleGKEGELLVRGQS 440
Cdd:cd17638 141 fETVLTAYGLTEAGVAtMCRPGDDAEtVATTCGRACPGFEVRI-------------------------ADDGEVLVRGYN 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 441 VFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaaDVRTLIDmlpgkgvnsaycsctirrcRGTLPreercq 520
Cdd:cd17638 196 VMQGYLDDPEATAEAIDADGWLHTG------------------DVGELDE-------------------RGYLR------ 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 521 tsfspmlqtgltptmt*ltslqrfahihplgdtaiykdgvywIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVI 600
Cdd:cd17638 233 ------------------------------------------ITDRLK-DMYIVGGFNVYPAEVEGALAEHPGVAQVAVI 269
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 928020699 601 GAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:cd17638 270 GVPDERMGEVGKAFVVARPGVTLTEEDVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
209-664 |
9.54e-36 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 138.38 E-value: 9.54e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATcVMLPDFQA- 287
Cdd:cd05944 2 DDVAAYFHTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGAH-VVLAGPAGy 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 288 ------QKVWEMLLSSKaptINVFMAVPTIYSKLIqfydqhyQQPLLKEFvkavckERIRLMVSGSAALPLPTLQRWKEI 361
Cdd:cd05944 81 rnpglfDNFWKLVERYR---ITSLSTVPTVYAALL-------QVPVNADI------SSLRFAMSGAAPLPVELRARFEDA 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 362 TGHTLLERYGMTEIGMALS-NPLTGPRIPGSVGVPLPGVDVRIVMNNKINTSIvegnh*ntwvRPGLEGKEGELLVRGQS 440
Cdd:cd05944 145 TGLPVVEGYGLTEATCLVAvNPPDGPKRPGSVGLRLPYARVRIKVLDGVGRLL----------RDCAPDEVGEICVAGPG 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 441 VFKEYWNKpQETRESFTDDGWFKTGTepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercq 520
Cdd:cd05944 215 VFGGYLYT-EGNKNAFVADGWLNTGD------------------------------------------------------ 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 521 tsfspmlqtgltptmt*ltslqrfahihpLGDtaIYKDGVYWIMGRtSVDILKSGGFKISALEVECHLLAHPDIADVAVI 600
Cdd:cd05944 240 -----------------------------LGR--LDADGYLFITGR-AKDLIIRGGHNIDPALIEEALLRHPAVAFAGAV 287
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 928020699 601 GAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMgPYK--IPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05944 288 GQPDAHAGELPVAYVQLKPGAVVEEEELLAWARDHV-PERaaVPKHIEVLEELPVTAVGKVFKPAL 352
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
209-664 |
1.07e-35 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 137.79 E-value: 1.07e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVML-PDFQA 287
Cdd:cd05917 2 DDVINIQFTSGTTGSPKGATLTHHNIVNNGYFIGERLGLTEQDRLCIPVPLFHCFGSVLGVLACLTHGATMVFPsPSFDP 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 288 QKVWEMLLSSKAPTINvfmAVPTIYSKLIQfydqhyqQPLLKEFVKAvckeRIRLMVSGSAALPLPTLQRWKEITGHT-L 366
Cdd:cd05917 82 LAVLEAIEKEKCTALH---GVPTMFIAELE-------HPDFDKFDLS----SLRTGIMAGAPCPPELMKRVIEVMNMKdV 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 367 LERYGMTE----IGMALSNPLTGPRIpGSVGVPLPGVDVRIVmnnKINTSIVegnh*ntwvrPGLeGKEGELLVRGQSVF 442
Cdd:cd05917 148 TIAYGMTEtspvSTQTRTDDSIEKRV-NTVGRIMPHTEAKIV---DPEGGIV----------PPV-GVPGELCIRGYSVM 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 443 KEYWNKPQETRESFTDDGWFKTgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqts 522
Cdd:cd05917 213 KGYWNDPEKTAEAIDGDGWLHT---------------------------------------------------------- 234
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 523 fspmlqtgltptmt*ltslqrfahihplGDTAIY-KDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIG 601
Cdd:cd05917 235 ----------------------------GDLAVMdEDGYCRIVGRIK-DMIIRGGENIYPREIEEFLHTHPKVSDVQVVG 285
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 928020699 602 APDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05917 286 VPDERYGEEVCAWIRLKEGAELTEEDIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKL 348
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
63-664 |
1.45e-35 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 141.44 E-value: 1.45e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 63 APEFGEKLAVIDSCGKHSYKQLYCSSLGLASRISsALGsdFGGLDgkRISFLCANDSSYVVAQWAVWMSGgtAVPLY--- 139
Cdd:COG1021 35 AERHPDRIAVVDGERRLSYAELDRRADRLAAGLL-ALG--LRPGD--RVVVQLPNVAEFVIVFFALFRAG--AIPVFalp 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 140 --RKHppsELEYIISDSQSSLVVA-----GHPYEEILQPLAQKLGLPCLP-----LPPTNNLDALDETDTREKEITITdw 207
Cdd:COG1021 108 ahRRA---EISHFAEQSEAVAYIIpdrhrGFDYRALARELQAEVPSLRHVlvvgdAGEFTSLDALLAAPADLSEPRPD-- 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 208 TDRPAMIIYTSGTTGRPKGVLHTHS----SIQAMtqclVSAWAWSKNDIilhtlplhhlhgivnkLLC------------ 271
Cdd:COG1021 183 PDDVAFFQLSGGTTGLPKLIPRTHDdylySVRAS----AEICGLDADTV----------------YLAalpaahnfplss 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 272 -----PLWVGATCVMLPDFQAQkvwEMLlsskaPTI-----NVFMAVPTIYSKLIQFYDQ-HYQQPLLkefvkavckeri 340
Cdd:COG1021 243 pgvlgVLYAGGTVVLAPDPSPD---TAF-----PLIerervTVTALVPPLALLWLDAAERsRYDLSSL------------ 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 341 RLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEiGMALSNPLTGPR--IPGSVGVPL-PGVDVRIVMNnkintsivEGN 417
Cdd:COG1021 303 RVLQVGGAKLSPELARRVRPALGCTLQQVFGMAE-GLVNYTRLDDPEevILTTQGRPIsPDDEVRIVDE--------DGN 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 418 h*ntwvrPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaaDVrtlidmlpgkgv 497
Cdd:COG1021 374 -------PVPPGEVGELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTG------------------DL------------ 416
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 498 nsaycsctIRRcrgtlpreercqtsfspmlqtglTPtmt*ltslqrfahihplgdtaiykDGVYWIMGRtSVDILKSGGF 577
Cdd:COG1021 417 --------VRR-----------------------TP------------------------DGYLVVEGR-AKDQINRGGE 440
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 578 KISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRkGKSLTLPELQTWARE-HMGPYKIPTGLLLVEEMPRNQM 656
Cdd:COG1021 441 KIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPR-GEPLTLAELRRFLRErGLAAFKLPDRLEFVDALPLTAV 519
|
....*...
gi 928020699 657 GKINKKDL 664
Cdd:COG1021 520 GKIDKKAL 527
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
68-664 |
3.96e-34 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 135.84 E-value: 3.96e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSEL 147
Cdd:cd05945 6 DRPAVVEGGRTLTYRELKERADALAAALASL-----GLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 148 EYIISDSQSSLVVAghpyeeilqplaqklglpclplpptnnldalDETDtrekeititdwtdrPAMIIYTSGTTGRPKGV 227
Cdd:cd05945 81 REILDAAKPALLIA-------------------------------DGDD--------------NAYIIFTSGSTGRPKGV 115
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 228 LHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGiVNKLLCPLWVGATCVMLPDFQAQKVWEMLLSSKAPTINVFMA 307
Cdd:cd05945 116 QISHDNLVSFTNWMLSDFPLGPGDVFLNQAPFSFDLS-VMDLYPALASGATLVPVPRDATADPKQLFRFLAEHGITVWVS 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 308 VPTIYSKLIQ---FYDQHYqqPLLKEFVkaVCKErirlmvsgsaALPLPTLQRWKEIT-GHTLLERYGMTEIGMALS--- 380
Cdd:cd05945 195 TPSFAAMCLLsptFTPESL--PSLRHFL--FCGE----------VLPHKTARALQQRFpDARIYNTYGPTEATVAVTyie 260
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 381 ---NPLTG-PRIPgsVGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGKEGELLVRGQSVFKEYWNKPQETRESF 456
Cdd:cd05945 261 vtpEVLDGyDRLP--IGYAKPGAKLVIL---------------DEDGRPVPPGEKGELVISGPSVSKGYLNNPEKTAAAF 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 457 -TDDG--WFKTGtepslsspafvlthfsaaDVRTLIDmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltp 533
Cdd:cd05945 324 fPDEGqrAYRTG------------------DLVRLEA------------------------------------------- 342
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 534 tmt*ltslqrfahihplgdtaiykDGVYWIMGRTSVDIlKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTA 613
Cdd:cd05945 343 ------------------------DGLLFYRGRLDFQV-KLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIA 397
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|..
gi 928020699 614 VVQLRKGKSLTLP-ELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05945 398 FVVPKPGAEAGLTkAIKAELAERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
104-664 |
2.70e-33 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 134.24 E-value: 2.70e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 104 GGLDGKRIS------FLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQPLAQKLG 177
Cdd:PRK06145 42 GMLHARGIGqgdvvaLLMKNSAAFLELAFAASYLGAVFLPINYRLAADEVAYILGDAGAKLLLVDEEFDAIVALETPKIV 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 178 LPCLPLPPTNNLDALDETDTREKEITITDWTdrpaMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTL 257
Cdd:PRK06145 122 IDAAAQADSRRLAQGGLEIPPQAAVAPTDLV----RLMYTSGTTDRPKGVMHSYGNLHWKSIDHVIALGLTASERLLVVG 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 258 PLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEMLLSSK------APTI-NVFMAVPTIYSkliqfYDQhyqqpllke 330
Cdd:PRK06145 198 PLYHVGAFDLPGIAVLWVGGTLRIHREFDPEAVLAAIERHRltcawmAPVMlSRVLTVPDRDR-----FDL--------- 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 331 fvkavckERIRLMVSGSAALPLPTLQRWKEI-TGHTLLERYGMTEIGMALSNPLTGPRIP--GSVGVPLPGVDVRIVmnn 407
Cdd:PRK06145 264 -------DSLAWCIGGGEKTPESRIRDFTRVfTRARYIDAYGLTETCSGDTLMEAGREIEkiGSTGRALAHVEIRIA--- 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 408 kintsivegNH*NTWVRPGLEGkegELLVRGQSVFKEYWNKPQETRESFTDDgWFKTGtepslsspafvlthfsaaDVRT 487
Cdd:PRK06145 334 ---------DGAGRWLPPNMKG---EICMRGPKVTKGYWKDPEKTAEAFYGD-WFRSG------------------DVGY 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 488 LIDmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYWIMGRT 567
Cdd:PRK06145 383 LDE-------------------------------------------------------------------EGFLYLTDRK 395
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 568 SvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLL 647
Cdd:PRK06145 396 K-DMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGATLTLEALDRHCRQRLASFKVPRQLKV 474
|
570
....*....|....*..
gi 928020699 648 VEEMPRNQMGKINKKDL 664
Cdd:PRK06145 475 RDELPRNPSGKVLKRVL 491
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
211-666 |
2.92e-33 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 134.52 E-value: 2.92e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 211 PAMIIYTSGTTGRPKGVLHTHSSI-QAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPlWVGATCVM---LPDFQ 286
Cdd:cd05928 176 PMAIYFTSGTTGSPKMAEHSHSSLgLGLKVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEP-WIQGACVFvhhLPRFD 254
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 287 AQKVWEMLlsSKAPtINVFMAVPTIYSKLIQFYDQHYQQPLLKEFVKAvckerirlmvsGSAALPlPTLQRWKEITGHTL 366
Cdd:cd05928 255 PLVILKTL--SSYP-ITTFCGAPTVYRMLVQQDLSSYKFPSLQHCVTG-----------GEPLNP-EVLEKWKAQTGLDI 319
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 367 LERYGMTEIGMALSNPLTGPRIPGSVGVPLPGVDVRIVMNNkintsiveGNh*ntwVRPglEGKEGELLVRGQ-----SV 441
Cdd:cd05928 320 YEGYGQTETGLICANFKGMKIKPGSMGKASPPYDVQIIDDN--------GN-----VLP--PGTEGDIGIRVKpirpfGL 384
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 442 FKEYWNKPQETRESFTDDgwfktgtepslsspaFVLThfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqt 521
Cdd:cd05928 385 FSGYVDNPEKTAATIRGD---------------FYLT------------------------------------------- 406
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 522 sfspmlqtgltptmt*ltslqrfahihplGDTAIY-KDGVYWIMGRtSVDILKSGGFKISALEVECHLLAHPDIADVAVI 600
Cdd:cd05928 407 -----------------------------GDRGIMdEDGYFWFMGR-ADDVINSSGYRIGPFEVESALIEHPAVVESAVV 456
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 928020699 601 GAPDATWGQKVTAVVQL------RKGKSLTLpELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLK 666
Cdd:cd05928 457 SSPDPIRGEVVKAFVVLapqflsHDPEQLTK-ELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRD 527
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
68-664 |
7.34e-33 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 132.05 E-value: 7.34e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSEL 147
Cdd:cd17643 2 EAVAVVDEDRRLTYGELDARANRLARTLRAE-----GVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVERI 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 148 EYIISDSQSSLVvaghpyeeilqplaqklglpclplpptnnldaldetdtrekeitITDwTDRPAMIIYTSGTTGRPKGV 227
Cdd:cd17643 77 AFILADSGPSLL--------------------------------------------LTD-PDDLAYVIYTSGSTGRPKGV 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 228 LHTHSSIQAM----------------TQCLVSAWAWSkndiilhtlplhhlhgiVNKLLCPLWVGATCVMLPDFQAQKVW 291
Cdd:cd17643 112 VVSHANVLALfaatqrwfgfneddvwTLFHSYAFDFS-----------------VWEIWGALLHGGRLVVVPYEVARSPE 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 292 EMLLSSKAPTINVFMAVPTIYSKLIQFYDQHYQQPLlkefvkavckeRIRLMVSGSAALPLPTLQRWKEITGH---TLLE 368
Cdd:cd17643 175 DFARLLRDEGVTVLNQTPSAFYQLVEAADRDGRDPL-----------ALRYVIFGGEALEAAMLRPWAGRFGLdrpQLVN 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 369 RYGMTEIGMALS-NPLTGPRIPGS----VGVPLPGVDVRIVMNNkintsivegnh*ntwVRPGLEGKEGELLVRGQSVFK 443
Cdd:cd17643 244 MYGITETTVHVTfRPLDAADLPAAaaspIGRPLPGLRVYVLDAD---------------GRPVPPGVVGELYVSGAGVAR 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 444 EYWNKPQETRESFTDDgwfktgtePslsspafvlthFSAADVRtlidmlpgkgvnsAYCSctirrcrgtlpreercqtsf 523
Cdd:cd17643 309 GYLGRPELTAERFVAN--------P-----------FGGPGSR-------------MYRT-------------------- 336
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 524 spmlqtgltptmt*ltslqrfahihplGDTAIYK-DGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGA 602
Cdd:cd17643 337 ---------------------------GDLARRLpDGELEYLGRAD-EQVKIRGFRIELGEIEAALATHPSVRDAAVIVR 388
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 928020699 603 PDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd17643 389 EDEPGDTRLVAYVVADDGAAADIAELRALLKELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
67-664 |
1.28e-32 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 131.64 E-value: 1.28e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 67 GEKLAVIDSCGKHSYKQLYCSSLGLASRISsalgsDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSE 146
Cdd:cd12116 1 PDATAVRDDDRSLSYAELDERANRLAARLR-----ARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADR 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 147 LEYIISDSQSSLVVAGhpyEEILQPLAQKLGLPclplpptnnLDALDETDTREKEITITDWTDRPAMIIYTSGTTGRPKG 226
Cdd:cd12116 76 LRYILEDAEPALVLTD---DALPDRLPAGLPVL---------LLALAAAAAAPAAPRTPVSPDDLAYVIYTSGSTGRPKG 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 227 VLHTHSSI----QAMTQCL----------VSAWAWsknDIilhtlplhhlhgIVNKLLCPLWVGATCVMLPD---FQAQK 289
Cdd:cd12116 144 VVVSHRNLvnflHSMRERLglgpgdrllaVTTYAF---DI------------SLLELLLPLLAGARVVIAPRetqRDPEA 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 290 VWEMLlssKAPTINVFMAVPTIYSKLIqfydqhYQQPLLKEFVKAVCkerirlmvsGSAALPlPTLQRWKEITGHTLLER 369
Cdd:cd12116 209 LARLI---EAHSITVMQATPATWRMLL------DAGWQGRAGLTALC---------GGEALP-PDLAARLLSRVGSLWNL 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 370 YGMTEIGM-ALSNPLTGPRIPGSVGVPLPGVDVRIVMNNkintsivegnh*ntwVRPGLEGKEGELLVRGQSVFKEYWNK 448
Cdd:cd12116 270 YGPTETTIwSTAARVTAAAGPIPIGRPLANTQVYVLDAA---------------LRPVPPGVPGELYIGGDGVAQGYLGR 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 449 PQETRESFTDDgwfktgtepslsspafvltHFSAADvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfSPMLQ 528
Cdd:cd12116 335 PALTAERFVPD-------------------PFAGPG---------------------------------------SRLYR 356
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 529 Tgltptmt*ltslqrfahihplGDTAIYK-DGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATw 607
Cdd:cd12116 357 T---------------------GDLVRRRaDGRLEYLGRAD-GQVKIRGHRIELGEIEAALAAHPGVAQAAVVVREDGG- 413
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*..
gi 928020699 608 GQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd12116 414 DRRLVAYVVLKAGAAPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
122-465 |
4.77e-32 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 128.92 E-value: 4.77e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQPLAQklglpcLPLPPTNNLDALDETDTREKE 201
Cdd:TIGR01733 39 VVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDSALASRLAGLVL------PVILLDPLELAALDDAPAPPP 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 202 ITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIIlhtlplHHLHGI-----VNKLLCPLWVG 276
Cdd:TIGR01733 113 PDAPSGPDDLAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLARRYGLDPDDRV------LQFASLsfdasVEEIFGALLAG 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 277 ATCVMLPDFQAQ---KVWEMLLSSKAptINVFMAVPTIYSKLIqfyDQHYQQPLlkefvkavckeRIRLMVSGSAALPLP 353
Cdd:TIGR01733 187 ATLVVPPEDEERddaALLAALIAEHP--VTVLNLTPSLLALLA---AALPPALA-----------SLRLVILGGEALTPA 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 354 TLQRWKEITGHT-LLERYGMTE-----IGMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGL 427
Cdd:TIGR01733 251 LVDRWRARGPGArLINLYGPTEttvwsTATLVDPDDAPRESPVPIGRPLANTRLYVL---------------DDDLRPVP 315
|
330 340 350 360
....*....|....*....|....*....|....*....|....*.
gi 928020699 428 EGKEGELLVRGQSVFKEYWNKPQETRESFTDDG--------WFKTG 465
Cdd:TIGR01733 316 VGVVGELYIGGPGVARGYLNRPELTAERFVPDPfaggdgarLYRTG 361
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
210-668 |
4.99e-32 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 126.68 E-value: 4.99e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 210 RPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCpLWVGATCVMLPDFQAqk 289
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWLLSLPLYHVGGLAILVRS-LLAGAELVLLERNQA-- 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 290 vwemLLSSKAPTINVFMA-VPTIYSKLIQfYDQHYQQPllkefvkavckERIRLMVSGSAALPLPTLQRWKEiTGHTLLE 368
Cdd:cd17630 78 ----LAEDLAPPGVTHVSlVPTQLQRLLD-SGQGPAAL-----------KSLRAVLLGGAPIPPELLERAAD-RGIPLYT 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 369 RYGMTEIGMALSNPLTGPRIPGSVGVPLPGVDVRIVMNnkintsivegnh*ntwvrpglegkeGELLVRGQSVFKEYWNK 448
Cdd:cd17630 141 TYGMTETASQVATKRPDGFGRGGVGVLLPGRELRIVED-------------------------GEIWVGGASLAMGYLRG 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 449 PqeTRESFTDDGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsaycsctirrcrgtlpreercqtsfspmlq 528
Cdd:cd17630 196 Q--LVPEFNEDGWFTTK------------------------DL------------------------------------- 212
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 529 tgltptmt*ltslqrfAHIHPlgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWG 608
Cdd:cd17630 213 ----------------GELHA--------DGRLTVLGRAD-NMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELG 267
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 609 QKVTAVVQLRKGksLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLKHF 668
Cdd:cd17630 268 QRPVAVIVGRGP--ADPAELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
68-664 |
3.81e-31 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 127.46 E-value: 3.81e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfGGLDGKRISfLCANDS-SYVVAQWAVWMSGGTAVPLYRKHPPSE 146
Cdd:cd17651 10 DAPALVAEGRRLTYAELDRRANRLAHRLRAR-----GVGPGDLVA-LCARRSaELVVALLAILKAGAAYVPLDPAYPAER 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 147 LEYIISDSQSSLVVAgHPYEEILQPLAqklglpclPLPPTNNLDALDETDTREKEITITDwTDRPAMIIYTSGTTGRPKG 226
Cdd:cd17651 84 LAFMLADAGPVLVLT-HPALAGELAVE--------LVAVTLLDQPGAAAGADAEPDPALD-ADDLAYVIYTSGSTGRPKG 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 227 VLHTHSSiqamtqcLVSAWAWSKNDI-ILHTLPLHHLHGI-----VNKLLCPLWVGATCVMLPDFQAQKVWEML--LSSK 298
Cdd:cd17651 154 VVMPHRS-------LANLVAWQARASsLGPGARTLQFAGLgfdvsVQEIFSTLCAGATLVLPPEEVRTDPPALAawLDEQ 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 299 APTInVFMavPTIYSK-LIQFYDQHYQQPLlkefvkavckeRIRLMVSGSAALPL-PTLQRW-KEITGHTLLERYGMTE- 374
Cdd:cd17651 227 RISR-VFL--PTVALRaLAEHGRPLGVRLA-----------ALRYLLTGGEQLVLtEDLREFcAGLPGLRLHNHYGPTEt 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 375 ---IGMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGKEGELLVRGQSVFKEYWNKPQE 451
Cdd:cd17651 293 hvvTALSLPGDPAAWPAPPPIGRPIDNTRVYVL---------------DAALRPVPPGVPGELYIGGAGLARGYLNRPEL 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 452 TRESFTDDGWfktgtepslsspafvlthfsaadvrtlidmLPGkgvnsaycsctirrcrgtlpreERcqtsfspMLQTGl 531
Cdd:cd17651 358 TAERFVPDPF------------------------------VPG----------------------AR-------MYRTG- 377
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 532 tptmt*ltSLQRFAhihplgdtaiyKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKV 611
Cdd:cd17651 378 --------DLARWL-----------PDGELEFLGRAD-DQVKIRGFRIELGEIEAALARHPGVREAVVLAREDRPGEKRL 437
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|...
gi 928020699 612 TAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd17651 438 VAYVVGDPEAPVDAAELRAALATHLPEYMVPSAFVLLDALPLTPNGKLDRRAL 490
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
122-664 |
3.89e-31 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 127.10 E-value: 3.89e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPyeeilqplaqklglpclplpptnnldaldetdtreke 201
Cdd:cd17649 51 VVALLAILKAGGAYVPLDPEYPAERLRYMLEDSGAGLLLTHHP------------------------------------- 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 202 ititdwtDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIIlHTLPLHHLHGIVNKLLCPLWVGATCVM 281
Cdd:cd17649 94 -------RQLAYVIYTSGSTGTPKGVAVSHGPLAAHCQATAERYGLTPGDRE-LQFASFNFDGAHEQLLPPLICGACVVL 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 282 LPDFQAQKVWEMLLSSKAPTINVfMAVPTIYSkliqfydqhYQQPLLKEFVKAVCKERIRLMVSGSAALPLPTLQRWKEI 361
Cdd:cd17649 166 RPDELWASADELAEMVRELGVTV-LDLPPAYL---------QQLAEEADRTGDGRPPSLRLYIFGGEALSPELLRRWLKA 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 362 tGHTLLERYGMTEigmALSNPLT----------GPRIPgsVGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGKE 431
Cdd:cd17649 236 -PVRLFNAYGPTE---ATVTPLVwkceagaaraGASMP--IGRPLGGRSAYIL---------------DADLNPVPVGVT 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 432 GELLVRGQSVFKEYWNKPQETRESFTDDgwfktgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrg 511
Cdd:cd17649 295 GELYIGGEGLARGYLGRPELTAERFVPD---------------------------------------------------- 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 512 tlpreercqtsfspmlqtgltPTMT*LTSLQRfahihpLGDTAIYK-DGVYWIMGRTSVDIlKSGGFKISALEVECHLLA 590
Cdd:cd17649 323 ---------------------PFGAPGSRLYR------TGDLARWRdDGVIEYLGRVDHQV-KIRGFRIELGEIEAALLE 374
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 928020699 591 HPDIADVAVIgAPDATWGQKVTAVVQLRKGKSL--TLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd17649 375 HPGVREAAVV-ALDGAGGKQLVAYVVLRAAAAQpeLRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKAL 449
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
61-664 |
7.80e-31 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 126.67 E-value: 7.80e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 61 TKAPEFGEKLAVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfgGLD-GKRISFLCANDSSYVVAQWAVWMSGGT---AV 136
Cdd:cd05920 23 RSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRGL------GIRpGDRVVVQLPNVAEFVVLFFALLRLGAVpvlAL 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 137 PLYRKHppsELEYIISDSQSSLVVAGhpyeeilqplaqklglpclplpptnnlDALDETDTREKEITITDWTDRPAMIIY 216
Cdd:cd05920 97 PSHRRS---ELSAFCAHAEAVAYIVP---------------------------DRHAGFDHRALARELAESIPEVALFLL 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 217 TSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHgivnKLLCP-----LWVGATCVMLPDFQAQKVW 291
Cdd:cd05920 147 SGGTTGTPKLIPRTHNDYAYNVRASAEVCGLDQDTVYLAVLPAAHNF----PLACPgvlgtLLAGGRVVLAPDPSPDAAF 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 292 EMLLSSKaptINVFMAVPtiyskliqfydqhyqqPLLKEFVKAVCKER-----IRLMVSGSAALPLPTLQRWKEITGHTL 366
Cdd:cd05920 223 PLIEREG---VTVTALVP----------------ALVSLWLDAAASRRadlssLRLLQVGGARLSPALARRVPPVLGCTL 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 367 LERYGMTEiGM----ALSNPltGPRIPGSVGVPL-PGVDVRIVMNnkintsivEGNh*ntwvrPGLEGKEGELLVRGQSV 441
Cdd:cd05920 284 QQVFGMAE-GLlnytRLDDP--DEVIIHTQGRPMsPDDEIRVVDE--------EGN-------PVPPGEEGELLTRGPYT 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 442 FKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaaDVrtlidmlpgkgvnsaycsctIRRcrgtlpreercqt 521
Cdd:cd05920 346 IRGYYRAPEHNARAFTPDGFYRTG------------------DL--------------------VRR------------- 374
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 522 sfspmlqtgltptmt*ltslqrfahiHPlgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIG 601
Cdd:cd05920 375 --------------------------TP--------DGYLVVEGRIK-DQINRGGEKIAAEEVENLLLRHPAVHDAAVVA 419
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 928020699 602 APDATWGQKVTAVVQLRkGKSLTLPELQTWAREhMG--PYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05920 420 MPDELLGERSCAFVVLR-DPPPSAAQLRRFLRE-RGlaAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
78-465 |
2.46e-30 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 124.78 E-value: 2.46e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 78 KHSYKQLYCSSLglasRISSALGSdFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSS 157
Cdd:cd17640 5 RITYKDLYQEIL----DFAAGLRS-LGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 158 LVVaghpyeeilqplaqklglpclplpptnnldaldeTDTREKEItitdwtdrpAMIIYTSGTTGRPKGVLHTHSSIqam 237
Cdd:cd17640 80 ALV----------------------------------VENDSDDL---------ATIIYTSGTTGNPKGVMLTHANL--- 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 238 TQCLVSAWAW---SKNDIILhtlplhhlhgivnkLLCPLW-------------VGATCV------MLPDFQAQKvwemll 295
Cdd:cd17640 114 LHQIRSLSDIvppQPGDRFL--------------SILPIWhsyersaeyfifaCGCSQAytsirtLKDDLKRVK------ 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 296 sskaPTinVFMAVPTIYSKLIQ-FYDQHYQQPLLKEFV--KAVCKERIRLMVSGSAALPlPTLQRWKEITGHTLLERYGM 372
Cdd:cd17640 174 ----PH--YIVSVPRLWESLYSgIQKQVSKSSPIKQFLflFFLSGGIFKFGISGGGALP-PHVDTFFEAIGIEVLNGYGL 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 373 TEIGMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnkintsiveGNH*NTWVRPGlegKEGELLVRGQSVFKEYWNKPQET 452
Cdd:cd17640 247 TETSPVVSARRLKCNVRGSVGRPLPGTEIKIV-----------DPEGNVVLPPG---EKGIVWVRGPQVMKGYYKNPEAT 312
|
410
....*....|...
gi 928020699 453 RESFTDDGWFKTG 465
Cdd:cd17640 313 SKVLDSDGWFNTG 325
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
80-465 |
9.34e-30 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 124.25 E-value: 9.34e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYcsslGLASRISSALGSDFG-GLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSL 158
Cdd:cd05927 7 SYKEVA----ERADNIGSALRSLGGkPAPASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEISI 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 159 VVAghpyeeilqplaqklglpclplpptnnldaldetdtrEKEITITDWTD---------------RP---AMIIYTSGT 220
Cdd:cd05927 83 VFC-------------------------------------DAGVKVYSLEEfeklgkknkvpppppKPedlATICYTSGT 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 221 TGRPKGVLHTHSSIqaMTQCLVSAWAWSKNDIILHTLPlhhlhgivnkLLC--PL---------------------WVGA 277
Cdd:cd05927 126 TGNPKGVMLTHGNI--VSNVAGVFKILEILNKINPTDV----------YISylPLahifervvealflyhgakigfYSGD 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 278 TCVMLPDFQAQKvwemllsskaPTInvFMAVPTIYSKL---IQFYDQhyQQPLLKEF----------------------- 331
Cdd:cd05927 194 IRLLLDDIKALK----------PTV--FPGVPRVLNRIydkIFNKVQ--AKGPLKRKlfnfalnyklaelrsgvvraspf 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 332 --------VKAVCKERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEI--GMALSNPltGPRIPGSVGVPLPGVDV 401
Cdd:cd05927 260 wdklvfnkIKQALGGNVRLMLTGSAPLSPEVLEFLRVALGCPVLEGYGQTECtaGATLTLP--GDTSVGHVGGPLPCAEV 337
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 928020699 402 RIVmnnkintSIVEGNH*NTWVRPglegkEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTG 465
Cdd:cd05927 338 KLV-------DVPEMNYDAKDPNP-----RGEVCIRGPNVFSGYYKDPEKTAEALDEDGWLHTG 389
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
71-664 |
1.52e-29 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 122.77 E-value: 1.52e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 71 AVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfgGLDGKRISFLCANDSS-YVVAQWAVWMSGGTAVPLYRKHPPSELEY 149
Cdd:cd17646 16 AVVDEGRTLTYRELDERANRLAHLLRAR------GVGPEDRVAVLLPRSAdLVVALLAVLKAGAAYLPLDPGYPADRLAY 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 150 IISDSQSSLVVAGHPYEEILQPLAqklglpCLPLPPTNNLDALDETDTREKEITitdwtDRPAMIIYTSGTTGRPKGVLH 229
Cdd:cd17646 90 MLADAGPAVVLTTADLAARLPAGG------DVALLGDEALAAPPATPPLVPPRP-----DNLAYVIYTSGSTGRPKGVMV 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 230 THssiqamtqclvsawawskndiilhtlplhhlHGIVNKLLC-----PLwvGATCVMLpdfqaQK--------VWEML-- 294
Cdd:cd17646 159 TH-------------------------------AGIVNRLLWmqdeyPL--GPGDRVL-----QKtplsfdvsVWELFwp 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 295 LSSKAptiNVFMAVP-----TIY-SKLIQFYD---QHYQQPLLKEFVKAVCKER---IRLMVSGSAALPLPTLQRWKEIT 362
Cdd:cd17646 201 LVAGA---RLVVARPgghrdPAYlAALIREHGvttCHFVPSMLRVFLAEPAAGScasLRRVFCSGEALPPELAARFLALP 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 363 GHTLLERYGMTE--IGM---ALSNPLTGPRIPgsVGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGKEGELLVR 437
Cdd:cd17646 278 GAELHNLYGPTEaaIDVthwPVRGPAETPSVP--IGRPVPNTRLYVL---------------DDALRPVPVGVPGELYLG 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 438 GQSVFKEYWNKPQETRESFTDDgWFKTGtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpree 517
Cdd:cd17646 341 GVQLARGYLGRPALTAERFVPD-PFGPG---------------------------------------------------- 367
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 518 rcqtsfSPMLQTGltptmt*ltSLQRFAHihplgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADV 597
Cdd:cd17646 368 ------SRMYRTG---------DLARWRP-----------DGALEFLGRSD-DQVKIRGFRVEPGEIEAALAAHPAVTHA 420
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 928020699 598 AVIGAPDATWGQKVTAVVQLRKGKS-LTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd17646 421 VVVARAAPAGAARLVGYVVPAAGAAgPDTAALRAHLAERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
191-465 |
1.85e-29 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 122.71 E-value: 1.85e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 191 ALDETdtrEKEITITDWT-DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTqclvsawawskndiilhtlplhhlHGIVNKL 269
Cdd:cd17639 72 SLNET---ECSAIFTDGKpDDLACIMYTSGSTGNPKGVMLTHGNLVAGI------------------------AGLGDRV 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 270 lcPLWVGAT---CVMLP-----DFQAQKV---WEMLL----------SSKA----------PTInvFMAVPTIY------ 312
Cdd:cd17639 125 --PELLGPDdryLAYLPlahifELAAENVclyRGGTIgygsprtltdKSKRgckgdltefkPTL--MVGVPAIWdtirkg 200
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 313 --SKLI-------QFYDQHYQ-----------QPLLKEFV----KAVCKERIRLMVSGSAALPLPTlQRWKEITGHTLLE 368
Cdd:cd17639 201 vlAKLNpmgglkrTLFWTAYQsklkalkegpgTPLLDELVfkkvRAALGGRLRYMLSGGAPLSADT-QEFLNIVLCPVIQ 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 369 RYGMTEI--GMALSNPltGPRIPGSVGVPLPGVDVRIVmnnkintSIVEGNH*NTWVRPglegkEGELLVRGQSVFKEYW 446
Cdd:cd17639 280 GYGLTETcaGGTVQDP--GDLETGRVGPPLPCCEIKLV-------DWEEGGYSTDKPPP-----RGEILIRGPNVFKGYY 345
|
330
....*....|....*....
gi 928020699 447 NKPQETRESFTDDGWFKTG 465
Cdd:cd17639 346 KNPEKTKEAFDGDGWFHTG 364
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
209-667 |
1.99e-29 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 122.99 E-value: 1.99e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMtqcLVSAWAWS---KNDIILHTLPLHHLHGIVNKLLCPlWVGATCVMLPDF 285
Cdd:cd05970 185 EDILLVYFSSGTTGMPKMVEHDFTYPLGH---IVTAKYWQnvrEGGLHLTVADTGWGKAVWGKIYGQ-WIAGAAVFVYDY 260
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 286 QAQKVWEMLLSSKAPTINVFMAVPTIYSKLIQFYDQHYQQPLLKEFVKAvckerirlmvsgSAALPLPTLQRWKEITGHT 365
Cdd:cd05970 261 DKFDPKALLEKLSKYGVTTFCAPPTIYRFLIREDLSRYDLSSLRYCTTA------------GEALNPEVFNTFKEKTGIK 328
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 366 LLERYGMTEIGMALSN-PLTGPRiPGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVR---GQSV 441
Cdd:cd05970 329 LMEGFGQTETTLTIATfPWMEPK-PGSMGKPAPGYEIDLIDREG---------------RSCEAGEEGEIVIRtskGKPV 392
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 442 --FKEYWNKPQETRESFtDDGWFKTGtepslsspafvlthfsaadvrtlidmlpgkgvNSAYcsctirrcrgtlpREErc 519
Cdd:cd05970 393 glFGGYYKDAEKTAEVW-HDGYYHTG--------------------------------DAAW-------------MDE-- 424
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 520 qtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAV 599
Cdd:cd05970 425 --------------------------------------DGYLWFVGRTD-DLIKSSGYRIGPFEVESALIQHPAVLECAV 465
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 928020699 600 IGAPDATWGQKVTAVVQLRK----GKSLTlPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLKH 667
Cdd:cd05970 466 TGVPDPIRGQVVKATIVLAKgyepSEELK-KELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIRER 536
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
34-661 |
2.07e-29 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 123.34 E-value: 2.07e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 34 LLEATVRRNSHRSALAPSWVEKqkpvftkapefgeklavidscgKHSYKQLycssLGLASRISSALGSdFGGLDGKRISF 113
Cdd:PRK12583 23 AFDATVARFPDREALVVRHQAL----------------------RYTWRQL----ADAVDRLARGLLA-LGVQPGDRVGI 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 114 LCANDSSYVVAQWAVWMSGGTAV---PLYRKhppSELEYIISDSQSSLVVA-----GHPYEEILQPLAQKLGLPCLPLPP 185
Cdd:PRK12583 76 WAPNCAEWLLTQFATARIGAILVninPAYRA---SELEYALGQSGVRWVICadafkTSDYHAMLQELLPGLAEGQPGALA 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 186 TNNLDALD-----------------------ETDTREK--EITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQC 240
Cdd:PRK12583 153 CERLPELRgvvslapapppgflawhelqargETVSREAlaERQASLDRDDPINIQYTSGTTGFPKGATLSHHNILNNGYF 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 241 LVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGAtCVMLPD--FQAQKVWEMLLSSKAPTINvfmAVPTIY-SKLiq 317
Cdd:PRK12583 233 VAESLGLTEHDRLCVPVPLYHCFGMVLANLGCMTVGA-CLVYPNeaFDPLATLQAVEEERCTALY---GVPTMFiAEL-- 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 318 fydqhyQQPLLKEFVKAvckeRIRLMVSGSAALPLPTLQR-WKEITGHTLLERYGMTEigmalSNPLTG--------PRI 388
Cdd:PRK12583 307 ------DHPQRGNFDLS----SLRTGIMAGAPCPIEVMRRvMDEMHMAEVQIAYGMTE-----TSPVSLqttaaddlERR 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 389 PGSVGVPLPGVDVRIVMNnkintsivEGNH*NTwvrpgleGKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtep 468
Cdd:PRK12583 372 VETVGRTQPHLEVKVVDP--------DGATVPR-------GEIGELCTRGYSVMKGYWNNPEATAESIDEDGWMHTG--- 433
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 469 slsspafvlthfsaaDVRTLIDmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahih 548
Cdd:PRK12583 434 ---------------DLATMDE---------------------------------------------------------- 440
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 549 plgdtaiykDGVYWIMGRtSVDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPEL 628
Cdd:PRK12583 441 ---------QGYVRIVGR-SKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRLHPGHAASEEEL 510
|
650 660 670
....*....|....*....|....*....|...
gi 928020699 629 QTWAREHMGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:PRK12583 511 REFCKARIAHFKVPRYFRFVDEFPMTVTGKVQK 543
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
63-658 |
2.26e-29 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 122.69 E-value: 2.26e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 63 APEFGEKLAVIdsCG--KHSYKQLYCSSLGLASRISSAlgsdfgGL-DGKRISFLCANDSSYVVAQWAVWMSGGTAVPL- 138
Cdd:PRK07798 13 ADAVPDRVALV--CGdrRLTYAELEERANRLAHYLIAQ------GLgPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVn 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 139 YRkHPPSELEYIISDSQSSLVVAGHPYEEILQPLAQKlglpclplppTNNLDAL----DETD--------TREKEITITD 206
Cdd:PRK07798 85 YR-YVEDELRYLLDDSDAVALVYEREFAPRVAEVLPR----------LPKLRTLvvveDGSGndllpgavDYEDALAAGS 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 207 wTDRPA--------MIIYTSGTTGRPKGVLHTHSSI--------QAMTQCLVSA-WAWSKNdiilhtlpLHHLHGIVNKL 269
Cdd:PRK07798 154 -PERDFgerspddlYLLYTGGTTGMPKGVMWRQEDIfrvllggrDFATGEPIEDeEELAKR--------AAAGPGMRRFP 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 270 LCPL------WV-------GATCVMLPD--FQAQKVWEMLLSSKAptiNVFMAVPTIYSKliqfydqhyqqPLLKEFVKA 334
Cdd:PRK07798 225 APPLmhgagqWAafaalfsGQTVVLLPDvrFDADEVWRTIEREKV---NVITIVGDAMAR-----------PLLDALEAR 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 335 vckER-----IRLMVSGSAALPLPTLQRWKEITGH-TLLERYGMTEIG-MALSNPLTGPRIPGSVGVPlPGVDVRIVMNN 407
Cdd:PRK07798 291 ---GPydlssLFAIASGGALFSPSVKEALLELLPNvVLTDSIGSSETGfGGSGTVAKGAVHTGGPRFT-IGPRTVVLDED 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 408 kinTSIVEgnh*ntwvrPGlEGKEGeLLVRGQSVFKEYWNKPQETRESFTddgwfktgtepslsspafvlthfsaadvrt 487
Cdd:PRK07798 367 ---GNPVE---------PG-SGEIG-WIARRGHIPLGYYKDPEKTAETFP------------------------------ 402
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 488 lidmlpgkgvnsaycscTIRRCRGTLPreercqtsfspmlqtgltptmt*ltslqrfahihplGDTA-IYKDGVYWIMGR 566
Cdd:PRK07798 403 -----------------TIDGVRYAIP------------------------------------GDRArVEADGTITLLGR 429
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 567 TSVDIlKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLL 646
Cdd:PRK07798 430 GSVCI-NTGGEKVFPEEVEEALKAHPDVADALVVGVPDERWGQEVVAVVQLREGARPDLAELRAHCRSSLAGYKVPRAIW 508
|
650
....*....|..
gi 928020699 647 LVEEMPRNQMGK 658
Cdd:PRK07798 509 FVDEVQRSPAGK 520
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
92-664 |
5.56e-29 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 121.34 E-value: 5.56e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 92 ASRISSALGSdFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAG----HPYEE 167
Cdd:PRK12406 21 AARAAGGLAA-LGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLIAHadllHGLAS 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 168 ILQPLAQKLGLPCLPLPPTN-NLDALDETdTREKEITITDW-----------TDRPAMIIYTSGTTGRPKGVLHTHSSI- 234
Cdd:PRK12406 100 ALPAGVTVLSVPTPPEIAAAyRISPALLT-PPAGAIDWEGWlaqqepydgppVPQPQSMIYTSGTTGHPKGVRRAAPTPe 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 235 QAMTQCLVSAWAWSkndiilhtlplhHLHGIVNKLLCPLW-------------VGATCVMLPDFQAQKVWEMLLSSKAPT 301
Cdd:PRK12406 179 QAAAAEQMRALIYG------------LKPGIRALLTGPLYhsapnayglragrLGGVLVLQPRFDPEELLQLIERHRITH 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 302 inVFMaVPTIYSKLIQfydqhyqqpLLKEFVKAVCKERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIG-MALS 380
Cdd:PRK12406 247 --MHM-VPTMFIRLLK---------LPEEVRAKYDVSSLRHVIHAAAPCPADVKRAMIEWWGPVIYEYYGSTESGaVTFA 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 381 NPLTGPRIPGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVR--GQSVFKeYWNKPqETRESFTD 458
Cdd:PRK12406 315 TSEDALSHPGTVGKAAPGAELRFVDEDG---------------RPLPQGEIGEIYSRiaGNPDFT-YHNKP-EKRAEIDR 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 459 DGWFKTGtepslsspafvlthfsaaDVrtlidmlpgkgvnsaycsctirrcrGTLPreercqtsfspmlqtgltptmt*l 538
Cdd:PRK12406 378 GGFITSG------------------DV-------------------------GYLD------------------------ 390
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 539 tslqrfahihplgdtaiyKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLR 618
Cdd:PRK12406 391 ------------------ADGYLFLCDRKR-DMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEPQ 451
|
570 580 590 600
....*....|....*....|....*....|....*....|....*.
gi 928020699 619 KGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK12406 452 PGATLDEADIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRL 497
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
105-664 |
6.16e-29 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 121.16 E-value: 6.16e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 105 GL-DGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQPLAQKLGLPCLPL 183
Cdd:PRK08276 32 GLrEGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLIVSAALADTAAELAAELPAGVPLL 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 184 PPT-------NNLDAL--DETDTRekeitITDWTDRPAMIiYTSGTTGRPKGVLH--THSSI---QAMTQCLVSAWAWSK 249
Cdd:PRK08276 112 LVVagpvpgfRSYEEAlaAQPDTP-----IADETAGADML-YSSGTTGRPKGIKRplPGLDPdeaPGMMLALLGFGMYGG 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 250 NDIILhtlplhhlhgivnklLCP---------------LWVGATCVMLPDFQAQKVWEMLLSSKaptINVFMAVPTIYSK 314
Cdd:PRK08276 186 PDSVY---------------LSPaplyhtaplrfgmsaLALGGTVVVMEKFDAEEALALIERYR---VTHSQLVPTMFVR 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 315 LIQfydqhyqqplLKEFVKAvckeR-----IRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTE-IGMALSNPLTGPRI 388
Cdd:PRK08276 248 MLK----------LPEEVRA----RydvssLRVAIHAAAPCPVEVKRAMIDWWGPIIHEYYASSEgGGVTVITSEDWLAH 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 389 PGSVGVPLPGVdVRIVMNNkintsiveGNH*NTwvrpgleGKEGELLV-RGQSVFkEYWNKPQETRESFTDDGWFKTGte 467
Cdd:PRK08276 314 PGSVGKAVLGE-VRILDED--------GNELPP-------GEIGTVYFeMDGYPF-EYHNDPEKTAAARNPHGWVTVG-- 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 468 pslsspafvlthfsaadvrtliDMlpgkgvnsaycsctirrcrGTLpreercqtsfspmlqtgltptmt*ltslqrfahi 547
Cdd:PRK08276 375 ----------------------DV-------------------GYL---------------------------------- 379
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 548 hplgDtaiyKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLT--- 624
Cdd:PRK08276 380 ----D----EDGYLYLTDRKS-DMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVQPADGADAGdal 450
|
570 580 590 600
....*....|....*....|....*....|....*....|
gi 928020699 625 LPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK08276 451 AAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRL 490
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
212-664 |
8.12e-29 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 121.41 E-value: 8.12e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 212 AMIIYTSGTTGRPKGVLHTHSSIQA-MTQC--LVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLP---DF 285
Cdd:PRK05677 210 AVLQYTGGTTGVAKGAMLTHRNLVAnMLQCraLMGSNLNEGCEILIAPLPLYHIYAFTFHCMAMMLIGNHNILISnprDL 289
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 286 QAqkvweMLLSSKAPTINVFMAVPTIYSKLIQfydqhyqqpllKEFVKAVCKERIRLMVSGSAALPLPTLQRWKEITGHT 365
Cdd:PRK05677 290 PA-----MVKELGKWKFSGFVGLNTLFVALCN-----------NEAFRKLDFSALKLTLSGGMALQLATAERWKEVTGCA 353
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 366 LLERYGMTEIG-MALSNPLTGPRIpGSVGVPLPGVDVRIVMNnkintsivEGNH*NTwvrpgleGKEGELLVRGQSVFKE 444
Cdd:PRK05677 354 ICEGYGMTETSpVVSVNPSQAIQV-GTIGIPVPSTLCKVIDD--------DGNELPL-------GEVGELCVKGPQVMKG 417
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 445 YWNKPQETRESFTDDGWFKTgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfs 524
Cdd:PRK05677 418 YWQRPEATDEILDSDGWLKT------------------------------------------------------------ 437
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 525 pmlqtgltptmt*ltslqrfahihplGDTA-IYKDGVYWIMGRTSVDILKSgGFKISALEVECHLLAHPDIADVAVIGAP 603
Cdd:PRK05677 438 --------------------------GDIAlIQEDGYMRIVDRKKDMILVS-GFNVYPNELEDVLAALPGVLQCAAIGVP 490
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 928020699 604 DATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK05677 491 DEKSGEAIKVFVVVKPGETLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRREL 551
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
108-664 |
2.27e-28 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 119.76 E-value: 2.27e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 108 GKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVV--AGHP-YEEILQPLAQKLGLPCLPLP 184
Cdd:PRK07470 57 GDRILVHSRNCNQMFESMFAAFRLGAVWVPTNFRQTPDEVAYLAEASGARAMIchADFPeHAAAVRAASPDLTHVVAIGG 136
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 185 PTNNLD---ALDETDTREKEITITDWtDRPAMIIYTSGTTGRPKGVLHTHSS------------IQAMTQ---CLVSAwa 246
Cdd:PRK07470 137 ARAGLDyeaLVARHLGARVANAAVDH-DDPCWFFFTSGTTGRPKAAVLTHGQmafvitnhladlMPGTTEqdaSLVVA-- 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 247 wskndiilhtlPLHHLHGIvnKLLCPLWVGATCVMLPD--FQAQKVWEmlLSSKAPTINVFmAVPTIYSKLIQF--YDQH 322
Cdd:PRK07470 214 -----------PLSHGAGI--HQLCQVARGAATVLLPSerFDPAEVWA--LVERHRVTNLF-TVPTILKMLVEHpaVDRY 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 323 YQQPLlkefvkavckeriRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIG-------MALSNPLTGP--RIpGSVG 393
Cdd:PRK07470 278 DHSSL-------------RYVIYAGAPMYRADQKRALAKLGKVLVQYFGLGEVTgnitvlpPALHDAEDGPdaRI-GTCG 343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 394 VPLPGVDVRIvmnnkintsivegnh*NTWVRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDdGWFKTGTepslssp 473
Cdd:PRK07470 344 FERTGMEVQI---------------QDDEGRELPPGETGEICVIGPAVFAGYYNNPEANAKAFRD-GWFRTGD------- 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 474 afvLTHFSAadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdt 553
Cdd:PRK07470 401 ---LGHLDA----------------------------------------------------------------------- 406
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 554 aiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAR 633
Cdd:PRK07470 407 ----RGFLYITGRAS-DMYISGGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVDEAELLAWLD 481
|
570 580 590
....*....|....*....|....*....|.
gi 928020699 634 EHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK07470 482 GKVARYKLPKRFFFWDALPKSGYGKITKKMV 512
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
71-664 |
2.63e-28 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 119.15 E-value: 2.63e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 71 AVIDSCGKH--SYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELE 148
Cdd:cd05923 19 AIADPARGLrlTYSELRARIEAVAARLHAR-----GLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKAAELA 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 149 YIISDSQSSLVVaghpyeeiLQPLAQKLGLPCLPLPPTNNLDALDetDTREKEI---TITDWTDRP---AMIIYTSGTTG 222
Cdd:cd05923 94 ELIERGEMTAAV--------IAVDAQVMDAIFQSGVRVLALSDLV--GLGEPESagpLIEDPPREPeqpAFVFYTSGTTG 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 223 RPKGVL--HTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEMLlssKAP 300
Cdd:cd05923 164 LPKGAVipQRAAESRVLFMSTQAGLRHGRHNVVLGLMPLYHVIGFFAVLVAALALDGTYVVVEEFDPADALKLI---EQE 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 301 TINVFMAVPTIYSKLIqfydQHYQQPLLKefvkavcKERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGMALS 380
Cdd:cd05923 241 RVTSLFATPTHLDALA----AAAEFAGLK-------LSSLRHVTFAGATMPDAVLERVNQHLPGEKVNIYGTTEAMNSLY 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 381 NPltGPRiPGSVGVPLPGVDVRIVmnnKINTSIVEGNH*ntwvrpgleGKEGELLVR--GQSVFKEYWNKPQETRESFTd 458
Cdd:cd05923 310 MR--DAR-TGTEMRPGFFSEVRIV---RIGGSPDEALAN---------GEEGELIVAaaADAAFTGYLNQPEATAKKLQ- 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 459 DGWFKTGtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*l 538
Cdd:cd05923 374 DGWYRTG------------------------------------------------------------------------- 380
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 539 tslqRFAHIHPLGDTAiykdgvywIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLR 618
Cdd:cd05923 381 ----DVGYVDPSGDVR--------ILGRVD-DMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPR 447
|
570 580 590 600
....*....|....*....|....*....|....*....|....*..
gi 928020699 619 KGkSLTLPELQTWARE-HMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd05923 448 EG-TLSADELDQFCRAsELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
92-472 |
2.74e-28 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 119.11 E-value: 2.74e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 92 ASRISSALGSDfgGLD-GKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGH------- 163
Cdd:cd05932 16 ARRLAAALRAL--GLEpGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFVGKlddwkam 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 164 ----PYEEILQPLAQKLGLPCLPLPPT--NNLDALDETDTREKEITITdwtdrpamIIYTSGTTGRPKGVLHTHSSIQAM 237
Cdd:cd05932 94 apgvPEGLISISLPPPSAANCQYQWDDliAQHPPLEERPTRFPEQLAT--------LIYTSGTTGQPKGVMLTFGSFAWA 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 238 TQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVM---LPDFQAQkvwemlLSSKAPTInvFMAVPTIYSK 314
Cdd:cd05932 166 AQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAFaesLDTFVED------VQRARPTL--FFSVPRLWTK 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 315 LIQ-FYDQHYQQP---LLK-EFVKAVCKERI---------RLMVSGSAALPlPTLQRWKEITGHTLLERYGMTEiGMALS 380
Cdd:cd05932 238 FQQgVQDKIPQQKlnlLLKiPVVNSLVKRKVlkglgldqcRLAGCGSAPVP-PALLEWYRSLGLNILEAYGMTE-NFAYS 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 381 NpLTGP--RIPGSVGVPLPGVDVRIvmnnkintsivegnh*ntwvrpgleGKEGELLVRGQSVFKEYWNKPQETRESFTD 458
Cdd:cd05932 316 H-LNYPgrDKIGTVGNAGPGVEVRI-------------------------SEDGEILVRSPALMMGYYKDPEATAEAFTA 369
|
410
....*....|....
gi 928020699 459 DGWFKTGTEPSLSS 472
Cdd:cd05932 370 DGFLRTGDKGELDA 383
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
71-664 |
4.27e-28 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 118.73 E-value: 4.27e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 71 AVIDSCGKHSYKQLYCSSLGLASRISSalgsdFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYI 150
Cdd:TIGR03098 18 ALVHHDRTLTYAALSERVLALASGLRG-----LGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPINPLLKAEQVAHI 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 151 ISDSQSSLVVAGHPYEEILQPLAQKLGLPCLPLPptnnLDALDETDTREKEITITDW----------------TDRPAMI 214
Cdd:TIGR03098 93 LADCNVRLLVTSSERLDLLHPALPGCHDLRTLII----VGDPAHASEGHPGEEPASWpkllalgdadpphpviDSDMAAI 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 215 IYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIvNKLLCPLWVGATCVMLpDFQAQKvwEML 294
Cdd:TIGR03098 169 LYTSGSTGRPKGVVLSHRNLVAGAQSVATYLENRPDDRLLAVLPLSFDYGF-NQLTTAFYVGATVVLH-DYLLPR--DVL 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 295 LSSKAPTINVFMAVPTIYSKLIQfydqhyqqplLKefVKAVCKERIRLMVSGSAALPLPTLQRWKEITGHT-LLERYGMT 373
Cdd:TIGR03098 245 KALEKHGITGLAAVPPLWAQLAQ----------LD--WPESAAPSLRYLTNSGGAMPRATLSRLRSFLPNArLFLMYGLT 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 374 EigmALSNPLTGP----RIPGSVGVPLPGVDVrIVMNNKintsiveGNH*ntwvrpgLEGKEGELLVRGQSVFKEYWNKP 449
Cdd:TIGR03098 313 E---AFRSTYLPPeevdRRPDSIGKAIPNAEV-LVLRED-------GSEC-------APGEEGELVHRGALVAMGYWNDP 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 450 QETRESFtddgwfktgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcRGTLPReercqtsfspmlQT 529
Cdd:TIGR03098 375 EKTAERF-----------------------------------------------------RPLPPF------------PG 389
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 530 GLTPTMT*LTSlqrfahihplGDTaIYKD--GVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATW 607
Cdd:TIGR03098 390 ELHLPELAVWS----------GDT-VRRDeeGFLYFVGRRD-EMIKTSGYRVSPTEVEEVAYATGLVAEAVAFGVPDPTL 457
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*..
gi 928020699 608 GQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:TIGR03098 458 GQAIVLVVTPPGGEELDRAALLAECRARLPNYMVPALIHVRQALPRNANGKIDRKAL 514
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
212-664 |
6.39e-28 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 118.62 E-value: 6.39e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 212 AMIIYTSGTTGRPKGVLHTHSSIQA-MTQClvsAWAWS------KNDIILHTLPLHHLHGIVNKLLCpLWVGATCVM--- 281
Cdd:PRK08974 209 AFLQYTGGTTGVAKGAMLTHRNMLAnLEQA---KAAYGpllhpgKELVVTALPLYHIFALTVNCLLF-IELGGQNLLitn 284
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 282 ---LPDFqaqkVWEMllsSKAPtinvFMA---VPTIYSKLIQFydqhyqqpllKEFvKAVCKERIRLMVSGSAALPLPTL 355
Cdd:PRK08974 285 prdIPGF----VKEL---KKYP----FTAitgVNTLFNALLNN----------EEF-QELDFSSLKLSVGGGMAVQQAVA 342
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 356 QRWKEITGHTLLERYGMTEIGMALS-NPLTGPRIPGSVGVPLPGVDVRIVMNnkintsivEGNh*ntwVRPglEGKEGEL 434
Cdd:PRK08974 343 ERWVKLTGQYLLEGYGLTECSPLVSvNPYDLDYYSGSIGLPVPSTEIKLVDD--------DGN-----EVP--PGEPGEL 407
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 435 LVRGQSVFKEYWNKPQETRESFTdDGWFKTGtepslsspafvlthfsaaDVRTLIDmlpgkgvnsaycsctirrcrgtlp 514
Cdd:PRK08974 408 WVKGPQVMLGYWQRPEATDEVIK-DGWLATG------------------DIAVMDE------------------------ 444
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 515 reercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYWIMGRTSVDILKSgGFKISALEVECHLLAHPDI 594
Cdd:PRK08974 445 -------------------------------------------EGFLRIVDRKKDMILVS-GFNVYPNEIEDVVMLHPKV 480
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 595 ADVAVIGAPDATWGQKVTAVVqLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK08974 481 LEVAAVGVPSEVSGEAVKIFV-VKKDPSLTEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRREL 549
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
34-668 |
6.65e-28 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 118.45 E-value: 6.65e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 34 LLEATVRRNSHRSALapswvekqkpVFTKapefgEKLAVidscgkhSYKQLYCSSLGLASRISSAlgsdfGGLDGKRISF 113
Cdd:PRK05852 21 LVEVAATRLPEAPAL----------VVTA-----DRIAI-------SYRDLARLVDDLAGQLTRS-----GLLPGDRVAL 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 114 LCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELE-----------YIISDSQSSLVVAGHPYEEILQPLAQKLGLPCLP 182
Cdd:PRK05852 74 RMGSNAEFVVALLAASRADLVVVPLDPALPIAEQRvrsqaagarvvLIDADGPHDRAEPTTRWWPLTVNVGGDSGPSGGT 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 183 LPPTnnLDALDETDTRekeiTITDWTDRP--AMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLH 260
Cdd:PRK05852 154 LSVH--LDAATEPTPA----TSTPEGLRPddAMIMFTGGTTGLPKMVPWTHANIASSVRAIITGYRLSPRDATVAVMPLY 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 261 HLHGIVNKLLCPLWVGATcVMLP---DFQAQKVWEMLlssKAPTINVFMAVPTIYSKLIQfydqhyqqpLLKEFVKAVCK 337
Cdd:PRK05852 228 HGHGLIAALLATLASGGA-VLLPargRFSAHTFWDDI---KAVGATWYTAVPTIHQILLE---------RAATEPSGRKP 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 338 ERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEI-------GMALSNPLTGPRI-PGSVGVPlPGVDVRIVMNNKi 409
Cdd:PRK05852 295 AALRFIRSCSAPLTAETAQALQTEFAAPVVCAFGMTEAthqvtttQIEGIGQTENPVVsTGLVGRS-TGAQIRIVGSDG- 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 410 ntsivegnh*ntwvRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTdDGWFKTGTEPSLSspafvlthfSAADVRtli 489
Cdd:PRK05852 373 --------------LPLPAGAVGEVWLRGTTVVRGYLGDPTITAANFT-DGWLRTGDLGSLS---------AAGDLS--- 425
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 490 dmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykdgvywIMGRTSv 569
Cdd:PRK05852 426 -------------------------------------------------------------------------IRGRIK- 431
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 570 DILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVE 649
Cdd:PRK05852 432 ELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVAAVIVPRESAPPTAEELVQFCRERLAAFEIPASFQEAS 511
|
650
....*....|....*....
gi 928020699 650 EMPRNQMGKINKKDLLKHF 668
Cdd:PRK05852 512 GLPHTAKGSLDRRAVAEQF 530
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
207-666 |
1.12e-27 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 118.07 E-value: 1.12e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 207 WTDRPAMII--YTSGTTGRPKGVLHTHssiQAMTQCLVSA-WA---------WSKNDiilHTLPLHHLHGIVNKLLCplw 274
Cdd:PRK04319 201 WTDREDGAIlhYTSGSTGKPKGVLHVH---NAMLQHYQTGkYVldlheddvyWCTAD---PGWVTGTSYGIFAPWLN--- 271
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 275 vGATCVML-PDFQAQKVWEMLLSSKaptINVFMAVPTIYSKLIQFYDqhyqqpllkEFVKAVCKERIRLMVS-GSaalPL 352
Cdd:PRK04319 272 -GATNVIDgGRFSPERWYRILEDYK---VTVWYTAPTAIRMLMGAGD---------DLVKKYDLSSLRHILSvGE---PL 335
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 353 -PTLQRW-KEITGHTLLERYGMTEIG-MALSNPLTGPRIPGSVGVPLPGVDVRIVMNNkintsiveGNh*ntwvrpGLE- 428
Cdd:PRK04319 336 nPEVVRWgMKVFGLPIHDNWWMTETGgIMIANYPAMDIKPGSMGKPLPGIEAAIVDDQ--------GN--------ELPp 399
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 429 GKEGELLVRG--QSVFKEYWNKPQETRESFTDdGWFKTGtepslsspafvlthfsaadvrtlidmlpgkgvNSAYcscti 506
Cdd:PRK04319 400 NRMGNLAIKKgwPSMMRGIWNNPEKYESYFAG-DWYVSG--------------------------------DSAY----- 441
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 507 rrcrgtlpREErcqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVEC 586
Cdd:PRK04319 442 --------MDE----------------------------------------DGYFWFQGRVD-DVIKTSGERVGPFEVES 472
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 587 HLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKG----KSLTLpELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKK 662
Cdd:PRK04319 473 KLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGyepsEELKE-EIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRR 551
|
....
gi 928020699 663 dLLK 666
Cdd:PRK04319 552 -VLK 554
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
209-465 |
1.81e-27 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 116.66 E-value: 1.81e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPD-FQA 287
Cdd:cd05909 147 DDPAVILFTSGSEGLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKVVFHPNpLDY 226
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 288 QKVWEMLLSSKAptiNVFMAVPTiyskliqfydqhyqqpLLKEFVKAVCKE---RIRLMVSGSAALPLPTLQRWKEITGH 364
Cdd:cd05909 227 KKIPELIYDKKA---TILLGTPT----------------FLRGYARAAHPEdfsSLRLVVAGAEKLKDTLRQEFQEKFGI 287
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 365 TLLERYGMTEIG--MALSNPLTgPRIPGSVGVPLPGVDVRIVMnnkintsiVEGNh*ntwvRPGLEGKEGELLVRGQSVF 442
Cdd:cd05909 288 RILEGYGTTECSpvISVNTPQS-PNKEGTVGRPLPGMEVKIVS--------VETH------EEVPIGEGGLLLVRGPNVM 352
|
250 260
....*....|....*....|...
gi 928020699 443 KEYWNKPQETRESFtDDGWFKTG 465
Cdd:cd05909 353 LGYLNEPELTSFAF-GDGWYDTG 374
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
67-659 |
2.73e-27 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 116.91 E-value: 2.73e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 67 GEKLAVI-----DSCGKH-SYKQLYCSSlglaSRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYR 140
Cdd:cd17634 67 GDRTAIIyegddTSQSRTiSYRELHREV----CRFAGTL-LDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFG 141
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 141 KHPPSELEYIISDSQSSLVVAGHPYEEI-----LQPLAQKLGLPCLPLPPTN------------------NLDALDETDT 197
Cdd:cd17634 142 GFAPEAVAGRIIDSSSRLLITADGGVRAgrsvpLKKNVDDALNPNVTSVEHVivlkrtgsdidwqegrdlWWRDLIAKAS 221
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 198 REKEITITDWTDrPAMIIYTSGTTGRPKGVLHTHSS-IQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVG 276
Cdd:cd17634 222 PEHQPEAMNAED-PLFILYTSGTTGKPKGVLHTTGGyLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHSYLLYGPLACG 300
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 277 ATCVML---PDF-QAQKVWEMLLSSKaptINVFMAVPTIYskliqfydqhyqQPLLKEFVKAVCK-ERIRLMVSGSAALP 351
Cdd:cd17634 301 ATTLLYegvPNWpTPARMWQVVDKHG---VNILYTAPTAI------------RALMAAGDDAIEGtDRSSLRILGSVGEP 365
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 352 L-PTLQRW--KEITGH--TLLERYGMTEIGMALSNPLTG--PRIPGSVGVPLPGVDVRIVMNnkintsivEGNh*ntwvr 424
Cdd:cd17634 366 InPEAYEWywKKIGKEkcPVVDTWWQTETGGFMITPLPGaiELKAGSATRPVFGVQPAVVDN--------EGH------- 430
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 425 PGLEGKEGELlvrgqsVFKEYWnkPQETRESFTDDgwfktgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsc 504
Cdd:cd17634 431 PQPGGTEGNL------VITDPW--PGQTRTLFGDH--------------------------------------------- 457
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 505 tirrcrgtlprEERCQTSFSpmlqtgltptmt*ltslqRFAHIHPLGDTA-IYKDGVYWIMGRtSVDILKSGGFKISALE 583
Cdd:cd17634 458 -----------ERFEQTYFS------------------TFKGMYFSGDGArRDEDGYYWITGR-SDDVINVAGHRLGTAE 507
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 928020699 584 VECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKG--KSLTL-PELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKI 659
Cdd:cd17634 508 IESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGvePSPELyAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKI 586
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
209-666 |
7.08e-27 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 115.67 E-value: 7.08e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIvNKLLCPLWVGATCVMLPDFQAQ 288
Cdd:PLN02860 172 DDAVLICFTSGTTGRPKGVTISHSALIVQSLAKIAIVGYGEDDVYLHTAPLCHIGGL-SSALAMLMVGACHVLLPKFDAK 250
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 289 KVWEMLlssKAPTINVFMAVPTIYSKLIQfydqhyqqpLLKEFVKAVCKERIRLMVSGSAALPLPTLQRWKEITGHT-LL 367
Cdd:PLN02860 251 AALQAI---KQHNVTSMITVPAMMADLIS---------LTRKSMTWKVFPSVRKILNGGGSLSSRLLPDAKKLFPNAkLF 318
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 368 ERYGMTEIG-----MALSNP-----------------LTGPRIPGS-VGVPLPGVDVRIVMNNKIntsivegnh*ntwvr 424
Cdd:PLN02860 319 SAYGMTEACssltfMTLHDPtlespkqtlqtvnqtksSSVHQPQGVcVGKPAPHVELKIGLDESS--------------- 383
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 425 pglegKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaaDVRTlIDmlpgkgvnsaycsc 504
Cdd:PLN02860 384 -----RVGRILTRGPHVMLGYWGQNSETASVLSNDGWLDTG------------------DIGW-ID-------------- 425
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 505 tirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiyKDGVYWIMGRTSvDILKSGGFKISALEV 584
Cdd:PLN02860 426 ----------------------------------------------------KAGNLWLIGRSN-DRIKTGGENVYPEEV 452
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 585 ECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKG------------KSLTLPE--LQTWARE-HMGPYKIPTGLLLVE 649
Cdd:PLN02860 453 EAVLSQHPGVASVVVVGVPDSRLTEMVVACVRLRDGwiwsdnekenakKNLTLSSetLRHHCREkNLSRFKIPKLFVQWR 532
|
490
....*....|....*...
gi 928020699 650 E-MPRNQMGKInKKDLLK 666
Cdd:PLN02860 533 KpFPLTTTGKI-RRDEVR 549
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
339-664 |
3.03e-26 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 113.38 E-value: 3.03e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 339 RIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIG-MALSNPLTGPRIPGSVGVPLPGVDVRIVMNNkintsivegn 417
Cdd:PRK12492 334 ALKLTNSGGTALVKATAERWEQLTGCTIVEGYGLTETSpVASTNPYGELARLGTVGIPVPGTALKVIDDD---------- 403
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 418 h*ntwvrpGLE---GKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTgtepslsspafvlthfsaadvrtlidmlpg 494
Cdd:PRK12492 404 --------GNElplGERGELCIKGPQVMKGYWQQPEATAEALDAEGWFKT------------------------------ 445
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 495 kgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplGDTA-IYKDGVYWIMGRTSvDILK 573
Cdd:PRK12492 446 --------------------------------------------------------GDIAvIDPDGFVRIVDRKK-DLII 468
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 574 SGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGkSLTLPELQTWAREHMGPYKIPTGLLLVEEMPR 653
Cdd:PRK12492 469 VSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVVARDP-GLSVEELKAYCKENFTGYKVPKHIVLRDSLPM 547
|
330
....*....|.
gi 928020699 654 NQMGKINKKDL 664
Cdd:PRK12492 548 TPVGKILRREL 558
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
211-659 |
3.67e-26 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 113.88 E-value: 3.67e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 211 PAMIIYTSGTTGRPKGVLHThsSIQAMTQCLVSAWA----------WSKNDIilhtlplhhlhGIVNK----LLCPLWVG 276
Cdd:TIGR02188 239 PLFILYTSGSTGKPKGVLHT--TGGYLLYAAMTMKYvfdikdgdifWCTADV-----------GWITGhsyiVYGPLANG 305
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 277 ATCVML---PDF-QAQKVWEMLLSSKaptINVFMAVPTIYSKLIQFYDQHyqqpllkefVKAVCKERIRLMvsGSAALPL 352
Cdd:TIGR02188 306 ATTVMFegvPTYpDPGRFWEIIEKHK---VTIFYTAPTAIRALMRLGDEW---------VKKHDLSSLRLL--GSVGEPI 371
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 353 -PTLQRW-KEITGHT---LLERYGMTEIGMALSNPLTG--PRIPGSVGVPLPGVDVRIVMNnkiNTSIVEGNH*ntwvrp 425
Cdd:TIGR02188 372 nPEAWMWyYKVVGKErcpIVDTWWQTETGGIMITPLPGatPTKPGSATLPFFGIEPAVVDE---EGNPVEGPG------- 441
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 426 glegkegellVRGQSVFKEYWnkPQETRESFTDDgwfktgtepslssPAFVLTHFSAAdvrtlidmlPGKgvnsaYcsct 505
Cdd:TIGR02188 442 ----------EGGYLVIKQPW--PGMLRTIYGDH-------------ERFVDTYFSPF---------PGY-----Y---- 478
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 506 irrcrgtlpreercqtsfspmlqtgltptmt*ltslqrFAhihplGDTAIY-KDGVYWIMGRTSvDILKSGGFKISALEV 584
Cdd:TIGR02188 479 --------------------------------------FT-----GDGARRdKDGYIWITGRVD-DVINVSGHRLGTAEI 514
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 928020699 585 ECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTL---PELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKI 659
Cdd:TIGR02188 515 ESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTLKDGYEPDDelrKELRKHVRKEIGPIAKPDKIRFVPGLPKTRSGKI 592
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
122-664 |
1.08e-25 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 110.42 E-value: 1.08e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAghpyeeilQPlaqklglpclplpptnnldaldetdtreke 201
Cdd:cd17652 51 VVAILAVLKAGAAYLPLDPAYPAERIAYMLADARPALLLT--------TP------------------------------ 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 202 ititdwtDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLhGIVNKLLCPLWVGATCVM 281
Cdd:cd17652 93 -------DNLAYVIYTSGSTGRPKGVVVTHRGLANLAAAQIAAFDVGPGSRVLQFASPSFD-ASVWELLMALLAGATLVL 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 282 LPD------------FQAQKVWEMLLSSKAptinvFMAVPtiyskliqfydqhyqqpllkefvkAVCKERIRLMVSGSAA 349
Cdd:cd17652 165 APAeellpgepladlLREHRITHVTLPPAA-----LAALP------------------------PDDLPDLRTLVVAGEA 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 350 LPLPTLQRWKeiTGHTLLERYGMTE--IGMALSNPLTGPRIPgSVGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGL 427
Cdd:cd17652 216 CPAELVDRWA--PGRRMINAYGPTEttVCATMAGPLPGGGVP-PIGRPVPGTRVYVL---------------DARLRPVP 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 428 EGKEGELLVRGQSVFKEYWNKPQETRESFTDDgwfktgtepslssPafvlthFSAadvrtlidmlPGkgvnsaycsctir 507
Cdd:cd17652 278 PGVPGELYIAGAGLARGYLNRPGLTAERFVAD-------------P------FGA----------PG------------- 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 508 rcrgtlpreERcqtsfspMLQTGltptmt*ltslqrfahihplgDTAIYK-DGVYWIMGRTSvDILKSGGFKISALEVEC 586
Cdd:cd17652 316 ---------SR-------MYRTG---------------------DLARWRaDGQLEFLGRAD-DQVKIRGFRIELGEVEA 357
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 928020699 587 HLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd17652 358 ALTEHPGVAEAVVVVRDDRPGDKRLVAYVVPAPGAAPTAAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
122-465 |
1.66e-25 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 112.64 E-value: 1.66e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQPLAQKLGlpclplpptnNLDALDETDTREKE 201
Cdd:COG1020 540 VVALLAVLKAGAAYVPLDPAYPAERLAYMLEDAGARLVLTQSALAARLPELGVPVL----------ALDALALAAEPATN 609
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 202 ITITDWTDRPAMIIYTSGTTGRPKGVLHTHSS----IQAMTQCL-----------------VSAWAwskndiilhtlplh 260
Cdd:COG1020 610 PPVPVTPDDLAYVIYTSGSTGRPKGVMVEHRAlvnlLAWMQRRYglgpgdrvlqfaslsfdASVWE-------------- 675
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 261 hlhgivnkLLCPLWVGATCVMLPD---FQAQKVWEMLLSSKaptINVFMAVPTIYSkliQFYDQHYQQPLlkefvkavck 337
Cdd:COG1020 676 --------IFGALLSGATLVLAPPearRDPAALAELLARHR---VTVLNLTPSLLR---ALLDAAPEALP---------- 731
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 338 eRIRLMVSGSAALPLPTLQRWKEITGHT-LLERYGMTE--IGMALSNPLTGPRIPGSV--GVPLPGVDVRIVmnnkints 412
Cdd:COG1020 732 -SLRLVLVGGEALPPELVRRWRARLPGArLVNLYGPTEttVDSTYYEVTPPDADGGSVpiGRPIANTRVYVL-------- 802
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 413 ivegnh*NTWVRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDDG-------WFKTG 465
Cdd:COG1020 803 -------DAHLQPVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPfgfpgarLYRTG 855
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
214-658 |
2.25e-25 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 108.24 E-value: 2.25e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 214 IIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGA------------TCVM 281
Cdd:cd05924 8 ILYTGGTTGMPKGVMWRQEDIFRMLMGGADFGTGEFTPSEDAHKAAAAAAGTVMFPAPPLMHGTgswtafggllggQTVV 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 282 LPD--FQAQKVWEMLLSSKAptiNVFMAVPTIYSKliqfydqhyqqPLLKEFVKA--VCKERIRLMVSGSAALPLPTLQR 357
Cdd:cd05924 88 LPDdrFDPEEVWRTIEKHKV---TSMTIVGDAMAR-----------PLIDALRDAgpYDLSSLFAISSGGALLSPEVKQG 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 358 WKEITGH-TLLERYGMTEIGMalsnpltgpripGSVGVPLPGVDVRIVMNNKINTSIVEGNH*NTwVRPGlEGKEGELLV 436
Cdd:cd05924 154 LLELVPNiTLVDAFGSSETGF------------TGSGHSAGSGPETGPFTRANPDTVVLDDDGRV-VPPG-SGGVGWIAR 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 437 RGQsVFKEYWNKPQETRESFtddgwfktgtepslsspafvlthFSAADVRTLIdmlpgkgvnsaycsctirrcrgtlpre 516
Cdd:cd05924 220 RGH-IPLGYYGDEAKTAETF-----------------------PEVDGVRYAV--------------------------- 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 517 ercqtsfspmlqtgltptmt*ltslqrfahihpLGDTAIY-KDGVYWIMGRTSVDIlKSGGFKISALEVECHLLAHPDIA 595
Cdd:cd05924 249 ---------------------------------PGDRATVeADGTVTLLGRGSVCI-NTGGEKVFPEEVEEALKSHPAVY 294
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 928020699 596 DVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGK 658
Cdd:cd05924 295 DVLVVGRPDERWGQEVVAVVQLREGAGVDLEELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
210-659 |
2.63e-25 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 107.11 E-value: 2.63e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 210 RPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLhGIVNKLLCPLWVGATCVMLPDFQAQK 289
Cdd:cd17633 1 NPFYIGFTSGTTGLPKAYYRSERSWIESFVCNEDLFNISGEDAILAPGPLSHS-LFLYGAISALYLGGTFIGQRKFNPKS 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 290 VWEMLLSSKAptINVFMaVPTIYSKLIQFYDQHyqqpllkefvkavckERIRLMVSGSAALPLPTLQRWKEITGHT-LLE 368
Cdd:cd17633 80 WIRKINQYNA--TVIYL-VPTMLQALARTLEPE---------------SKIKSIFSSGQKLFESTKKKLKNIFPKAnLIE 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 369 RYGMTEIGMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnkintsivegNH*NtwvrpgleGKEGELLVRGQSVFKEYWNK 448
Cdd:cd17633 142 FYGTSELSFITYNFNQESRPPNSVGRPFPNVEIEIR------------NADG--------GEIGKIFVKSEMVFSGYVRG 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 449 pqetrESFTDDGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsAYcsctirrcrgtlpreercqtsfspmlq 528
Cdd:cd17633 202 -----GFSNPDGWMSVG------------------------DI--------GY--------------------------- 217
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 529 tgltptmt*ltslqrfahihplgdtaIYKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWG 608
Cdd:cd17633 218 --------------------------VDEEGYLYLVGRES-DMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFG 270
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|.
gi 928020699 609 QKVTAVVqlrKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKI 659
Cdd:cd17633 271 EIAVALY---SGDKLTYKQLKRFLKQKLSRYEIPKKIIFVDSLPYTSSGKI 318
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
67-664 |
3.71e-25 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 109.60 E-value: 3.71e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 67 GEKLAVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSE 146
Cdd:cd12117 11 PDAVAVVYGDRSLTYAELNERANRLARRLRAA-----GVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPELPAER 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 147 LEYIISDSQSSLVVAGHPYEEILQPLAQKLGLpclplpptnnLDALDETDTREKEITITdwTDRPAMIIYTSGTTGRPKG 226
Cdd:cd12117 86 LAFMLADAGAKVLLTDRSLAGRAGGLEVAVVI----------DEALDAGPAGNPAVPVS--PDDLAYVMYTSGSTGRPKG 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 227 VLHTHSSIQAMtqCLVSAWAwskndiilhtlplHHLHGIVNKLLCPL---------WV----GATCVMLPD--------- 284
Cdd:cd12117 154 VAVTHRGVVRL--VKNTNYV-------------TLGPDDRVLQTSPLafdastfeiWGallnGARLVLAPKgtlldpdal 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 285 ---FQAQKVWEMLLSskAPTINvfmavptiyskliQFYDQHYQqpllkefvkavCKERIRLMVSGSAALPLPTLQRWKEI 361
Cdd:cd12117 219 galIAEEGVTVLWLT--AALFN-------------QLADEDPE-----------CFAGLRELLTGGEVVSPPHVRRVLAA 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 362 TGH-TLLERYGMTE-IGMALSNPLT-----GPRIPgsVGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGKEGEL 434
Cdd:cd12117 273 CPGlRLVNGYGPTEnTTFTTSHVVTeldevAGSIP--IGRPIANTRVYVL---------------DEDGRPVPPGVPGEL 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 435 LVRGQSVFKEYWNKPQETRESFTDDGWfktgtepslsspafvlthfsaadvrtlidmLPGkgvnsaycsctirrcrGTLP 514
Cdd:cd12117 336 YVGGDGLALGYLNRPALTAERFVADPF------------------------------GPG----------------ERLY 369
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 515 ReercqtsfspmlqTGltptmt*ltslqRFAHIHPlgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDI 594
Cdd:cd12117 370 R-------------TG------------DLARWLP--------DGRLEFLGRID-DQVKIRGFRIELGEIEAALRAHPGV 415
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 595 ADVAVIGAPDATWGQKVTAVVqlRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd12117 416 REAVVVVREDAGGDKRLVAYV--VAEGALDAAELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
90-665 |
8.07e-25 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 108.30 E-value: 8.07e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 90 GLASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGT----AVPLYRKHPPSELEYIISDSQSSLVVAGHPY 165
Cdd:cd05922 1 LGVSAAASAL-LEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRlglvFVPLNPTLKESVLRYLVADAGGRIVLADAGA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 166 EEILQPLAqklglpCLPLPPTNNLDALDETDTREKEITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAW 245
Cdd:cd05922 80 ADRLRDAL------PASPDPGTVLDADGIRAARASAPAHEVSHEDLALLLYTSGSTGSPKLVRLSHQNLLANARSIAEYL 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 246 AWSKNDIILHTLPLHHLHGIvNKLLCPLWVGATCVMLPDFQ-AQKVWEMLlssKAPTINVFMAVPTIYSKL--IQFYDQH 322
Cdd:cd05922 154 GITADDRALTVLPLSYDYGL-SVLNTHLLRGATLVLTNDGVlDDAFWEDL---REHGATGLAGVPSTYAMLtrLGFDPAK 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 323 YqqPLLkefvkavckeriRLMVSGSAALPLPTLQRWKE-ITGHTLLERYGMTEI--GMALSNPLTGPRIPGSVGVPLPGV 399
Cdd:cd05922 230 L--PSL------------RYLTQAGGRLPQETIARLRElLPGAQVYVMYGQTEAtrRMTYLPPERILEKPGSIGLAIPGG 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 400 DVRIVMNnkintsivEGNH*NTwvrpgleGKEGELLVRGQSVFKEYWNKPQETREsftddgwfKTGTEPSLSSpafvlth 479
Cdd:cd05922 296 EFEILDD--------DGTPTPP-------GEPGEIVHRGPNVMKGYWNDPPYRRK--------EGRGGGVLHT------- 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 480 fsaadvrtlidmlpgkgvnsaycsctirrcrGTLPREErcqtsfspmlqtgltptmt*ltslqrfahihplgdtaiyKDG 559
Cdd:cd05922 346 -------------------------------GDLARRD---------------------------------------EDG 355
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 560 VYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDaTWGQKVTAVVQLRKGksLTLPELQTWAREHMGPY 639
Cdd:cd05922 356 FLFIVGRRD-RMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPD-PLGEKLALFVTAPDK--IDPKDVLRSLAERLPPY 431
|
570 580
....*....|....*....|....*.
gi 928020699 640 KIPTGLLLVEEMPRNQMGKINKKDLL 665
Cdd:cd05922 432 KVPATVRVVDELPLTASGKVDYAALR 457
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
211-661 |
9.80e-25 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 105.81 E-value: 9.80e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 211 PAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSA-WAWSKNDIILHTLPLHHLHGIVNKLLCpLWVGATCVMLPDFQAQK 289
Cdd:cd17635 3 PLAVIFTSGTTGEPKAVLLANKTFFAVPDILQKEgLNWVVGDVTYLPLPATHIGGLWWILTC-LIHGGLCVTGGENTTYK 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 290 VWEMLLSSKAPTINVFmaVPTIYSKLIQfydqhyqqpLLKEFVKAVckERIRLMVSGSaALPLPTLQRWKEITGHT-LLE 368
Cdd:cd17635 82 SLFKILTTNAVTTTCL--VPTLLSKLVS---------ELKSANATV--PSLRLIGYGG-SRAIAADVRFIEATGLTnTAQ 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 369 RYGMTEIGMALSNPL-TGPRIPGSVGVPLPGVDVRIVMNNKIntsivegnh*ntwvrPGLEGKEGELLVRGQSVFKEYWN 447
Cdd:cd17635 148 VYGLSETGTALCLPTdDDSIEINAVGRPYPGVDVYLAATDGI---------------AGPSASFGTIWIKSPANMLGYWN 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 448 KPQETRESFTdDGWFKTGtepslsspafvlthfsaadvrtliDMLpgkgvnsaycsctirrcrgtlpreercqtsfspml 527
Cdd:cd17635 213 NPERTAEVLI-DGWVNTG------------------------DLG----------------------------------- 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 528 qtgltptmt*ltslqrfahihplgdtAIYKDGVYWIMGRTSVDILKsGGFKISALEVECHLLAHPDIADVAVIGAPDATW 607
Cdd:cd17635 233 --------------------------ERREDGFLFITGRSSESINC-GGVKIAPDEVERIAEGVSGVQECACYEISDEEF 285
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*....
gi 928020699 608 GqkvtAVVQLRKGKSLTLPE-----LQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:cd17635 286 G----ELVGLAVVASAELDEnairaLKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
122-664 |
1.77e-24 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 107.41 E-value: 1.77e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEeilQPLAqklglpclplpPTNNLDALDETDTREKE 201
Cdd:cd17655 61 IVGILGILKAGGAYLPIDPDYPEERIQYILEDSGADILLTQSHLQ---PPIA-----------FIGLIDLLDEDTIYHEE 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 202 ITITDWTDRP---AMIIYTSGTTGRPKGVLHTHSSiqamtqclVSAWAWSKNDIILHTLPLHHLH-------GIVNKLLC 271
Cdd:cd17655 127 SENLEPVSKSddlAYVIYTSGSTGKPKGVMIEHRG--------VVNLVEWANKVIYQGEHLRVALfasisfdASVTEIFA 198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 272 PLWVGATCVMLPDFQAQKVWEMLLSSKAPTINVFMAVPTiyskliqfydqHYQqpLLKEfVKAVCKERIRLMVSGSAALP 351
Cdd:cd17655 199 SLLSGNTLYIVRKETVLDGQALTQYIRQNRITIIDLTPA-----------HLK--LLDA-ADDSEGLSLKHLIVGGEALS 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 352 LPTLQRWKEITGH--TLLERYGMTE------IGMALSNPLTGPRIPgsVGVPLPGVDVRIVmnnkintsivegnh*NTWV 423
Cdd:cd17655 265 TELAKKIIELFGTnpTITNAYGPTEttvdasIYQYEPETDQQVSVP--IGKPLGNTRIYIL---------------DQYG 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 424 RPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDDgwfktgtepslsspAFVlthfsaadvrtlidmlPGkgvnsaycs 503
Cdd:cd17655 328 RPQPVGVAGELYIGGEGVARGYLNRPELTAEKFVDD--------------PFV----------------PG--------- 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 504 ctirrcrgtlpreERcqtsfspMLQTgltptmt*ltslqrfahihplGDTAIY-KDGVYWIMGRTSvDILKSGGFKISAL 582
Cdd:cd17655 369 -------------ER-------MYRT---------------------GDLARWlPDGNIEFLGRID-HQVKIRGYRIELG 406
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 583 EVECHLLAHPDIADVAVIGAPDATWGQKVTA-VVQlrkGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:cd17655 407 EIEARLLQHPDIKEAVVIARKDEQGQNYLCAyIVS---EKELPVAQLREFLARELPDYMIPSYFIKLDEIPLTPNGKVDR 483
|
...
gi 928020699 662 KDL 664
Cdd:cd17655 484 KAL 486
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
2-664 |
6.80e-24 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 107.94 E-value: 6.80e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 2 LPELFAFSKQTLMwtSRWKATPYRTLFYSRQRLLEATVRRNSHRSALApswvekqkpvftkapeFGEKLAvidscgkhSY 81
Cdd:PRK12467 487 LPLLDAEERAREL--VRWNAPATEYAPDCVHQLIEAQARQHPERPALV----------------FGEQVL--------SY 540
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 82 KQLYCSSLGLASR-ISSALGSDfggldgKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVV 160
Cdd:PRK12467 541 AELNRQANRLAHVlIAAGVGPD------VLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQDRLAYMLDDSGVRLLL 614
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 161 aGHPYEEILQPLAQKLGLPCLPlpptnnlDALDETDTREKEITITDWT-DRPAMIIYTSGTTGRPKGVLHTHSSIqAMTQ 239
Cdd:PRK12467 615 -TQSHLLAQLPVPAGLRSLCLD-------EPADLLCGYSGHNPEVALDpDNLAYVIYTSGSTGQPKGVAISHGAL-ANYV 685
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 240 CLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEMLLSSKAPTINVFMAVPTiyskliqfy 319
Cdd:PRK12467 686 CVIAERLQLAADDSMLMVSTFAFDLGVTELFGALASGATLHLLPPDCARDAEAFAALMADQGVTVLKIVPS--------- 756
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 320 dqHYQQpLLKEFVKAVCKERIRLMVSGSaALPLPTLQRWKEIT-GHTLLERYGMTEIGMALSN-PLTG-PRIPGSV--GV 394
Cdd:PRK12467 757 --HLQA-LLQASRVALPRPQRALVCGGE-ALQVDLLARVRALGpGARLINHYGPTETTVGVSTyELSDeERDFGNVpiGQ 832
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 395 PLPGVDVRIvMNNKINtsivegnh*ntwvrPGLEGKEGELLVRGQSVFKEYWNKPQETRESFtddgwfktgtepsLSSPa 474
Cdd:PRK12467 833 PLANLGLYI-LDHYLN--------------PVPVGVVGELYIGGAGLARGYHRRPALTAERF-------------VPDP- 883
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 475 fvlthFSAADVRtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspMLQTgltptmt*ltslqrfahihplGDTA 554
Cdd:PRK12467 884 -----FGADGGR---------------------------------------LYRT---------------------GDLA 898
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 555 IY-KDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKG----KSLTLPELQ 629
Cdd:PRK12467 899 RYrADGVIEYLGRMD-HQVKIRGFRIELGEIEARLLAQPGVREAVVLAQPGDAGLQLVAYLVPAAVAdgaeHQATRDELK 977
|
650 660 670
....*....|....*....|....*....|....*
gi 928020699 630 TWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK12467 978 AQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKAL 1012
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
80-465 |
7.93e-24 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 105.83 E-value: 7.93e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYCSslglASRISSALGSDfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPL-----YRKHPPSE--LEYIIS 152
Cdd:cd05906 41 SYQDLLED----ARRLAAGLRQL-GLRPGDSVILQFDDNEDFIPAFWACVLAGFVPAPLtvpptYDEPNARLrkLRHIWQ 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 153 DSQSSLVVAGhpyEEILQPLAQKLGLPCLPLPPTNNLDALDETDtREKEITITDWTDrPAMIIYTSGTTGRPKGVLHTHS 232
Cdd:cd05906 116 LLGSPVVLTD---AELVAEFAGLETLSGLPGIRVLSIEELLDTA-ADHDLPQSRPDD-LALLMLTSGSTGFPKAVPLTHR 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 233 SIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPdfqaqkvwemllsskAPTInvfMAVPTIY 312
Cdd:cd05906 191 NILARSAGKIQHNGLTPQDVFLNWVPLDHVGGLVELHLRAVYLGCQQVHVP---------------TEEI---LADPLRW 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 313 SKLIQfydqHYQ-----QP-----LLKEFVKAVCKER-----IRLMVSGSAALPLPTLQRWKEitghtLLERY------- 370
Cdd:cd05906 253 LDLID----RYRvtitwAPnfafaLLNDLLEEIEDGTwdlssLRYLVNAGEAVVAKTIRRLLR-----LLEPYglppdai 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 371 ----GMTEI--GMALSNPLTGPRIPG-----SVGVPLPGVDVRIVmnnKINTSIVEgnh*ntwvrpglEGKEGELLVRGQ 439
Cdd:cd05906 324 rpafGMTETcsGVIYSRSFPTYDHSQalefvSLGRPIPGVSMRIV---DDEGQLLP------------EGEVGRLQVRGP 388
|
410 420
....*....|....*....|....*.
gi 928020699 440 SVFKEYWNKPQETRESFTDDGWFKTG 465
Cdd:cd05906 389 VVTKGYYNNPEANAEAFTEDGWFRTG 414
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
211-659 |
9.41e-24 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 106.11 E-value: 9.41e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 211 PAMIIYTSGTTGRPKGVLHTHS---SIQAMTqclvSAWA---------WSKNDIilhtlplhhlhG-------IVnklLC 271
Cdd:cd05966 233 PLFILYTSGSTGKPKGVVHTTGgylLYAATT----FKYVfdyhpddiyWCTADI-----------GwitghsyIV---YG 294
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 272 PLWVGATCVML---PDF-QAQKVWEMLLSSKaptINVFMAVPTIYSKLIQFYDqhyqqpllkEFVKAVCKERIRLMvsGS 347
Cdd:cd05966 295 PLANGATTVMFegtPTYpDPGRYWDIVEKHK---VTIFYTAPTAIRALMKFGD---------EWVKKHDLSSLRVL--GS 360
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 348 AALPL-PTLQRW-KEITGH---TLLERYGMTEIGMALSNPLTG--PRIPGSVGVPLPGVDVRIVMNNkinTSIVEGNH*n 420
Cdd:cd05966 361 VGEPInPEAWMWyYEVIGKercPIVDTWWQTETGGIMITPLPGatPLKPGSATRPFFGIEPAILDEE---GNEVEGEV-- 435
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 421 twvrpglegkEGELlvrgqsVFKEYWnkPQETRESFTDDGwfktgtepslsspAFVLTHFSAadvrtlidmLPGKgvnsa 500
Cdd:cd05966 436 ----------EGYL------VIKRPW--PGMARTIYGDHE-------------RYEDTYFSK---------FPGY----- 470
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 501 YCSctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplGDTAIY-KDGVYWIMGRTSvDILKSGGFKI 579
Cdd:cd05966 471 YFT-----------------------------------------------GDGARRdEDGYYWITGRVD-DVINVSGHRL 502
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 580 SALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLP---ELQTWAREHMGPYKIPTGLLLVEEMPRNQM 656
Cdd:cd05966 503 GTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEPSDElrkELRKHVRKEIGPIATPDKIQFVPGLPKTRS 582
|
...
gi 928020699 657 GKI 659
Cdd:cd05966 583 GKI 585
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
212-664 |
9.99e-24 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 105.87 E-value: 9.99e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 212 AMIIYTSGTTGRPKGVLHTHSSIQA-MTQclVSAW---AWSKNDIILHTLplhhlhgIVNKLlcPLW------------- 274
Cdd:PRK07059 207 AFLQYTGGTTGVSKGATLLHRNIVAnVLQ--MEAWlqpAFEKKPRPDQLN-------FVCAL--PLYhifaltvcgllgm 275
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 275 -VGATCVMLPDfqAQKVWEMLLSSKAPTINVFMAVPTIYSKLIQfydqhyqQPllkEFVKAVCKeRIRLMVSGSAALPLP 353
Cdd:PRK07059 276 rTGGRNILIPN--PRDIPGFIKELKKYQVHIFPAVNTLYNALLN-------NP---DFDKLDFS-KLIVANGGGMAVQRP 342
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 354 TLQRWKEITGHTLLERYGMTEIG-MALSNPLTGPRIPGSVGVPLPGVDVRIVMNnkintsivEGNH*NTwvrpgleGKEG 432
Cdd:PRK07059 343 VAERWLEMTGCPITEGYGLSETSpVATCNPVDATEFSGTIGLPLPSTEVSIRDD--------DGNDLPL-------GEPG 407
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 433 ELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaadvrtliDMlpgkGVNSAycsctirrcrgt 512
Cdd:PRK07059 408 EICIRGPQVMAGYWNRPDETAKVMTADGFFRTG------------------------DV----GVMDE------------ 447
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 513 lpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYWIMGRTSVDILKSgGFKISALEVECHLLAHP 592
Cdd:PRK07059 448 ---------------------------------------------RGYTKIVDRKKDMILVS-GFNVYPNEIEEVVASHP 481
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 928020699 593 DIADVAVIGAPDATWGQKVTAVVqLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK07059 482 GVLEVAAVGVPDEHSGEAVKLFV-VKKDPALTEEDVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRREL 552
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
91-661 |
1.34e-23 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 104.14 E-value: 1.34e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 91 LASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAghpyeeilq 170
Cdd:cd05973 9 LSARFANAL-QELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVT--------- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 171 plaqklglpclplpptnNLDALDETDtrekeititdwtDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKN 250
Cdd:cd05973 79 -----------------DAANRHKLD------------SDPFVMMFTSGTTGLPKGVPVPLRALAAFGAYLRDAVDLRPE 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 251 DIILHTLPLHHLHGIVNKLLCPLWVGATCVMLP-DFQAQKVWEMLlssKAPTINVFMAVPTIYskliqfydqhyqQPLLK 329
Cdd:cd05973 130 DSFWNAADPGWAYGLYYAITGPLALGHPTILLEgGFSVESTWRVI---ERLGVTNLAGSPTAY------------RLLMA 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 330 EFVKAVCKERIRLMVSGSAALPL-PTLQRWKEIT-GHTLLERYGMTEIGMALSN--PLTGPRIPGSVGVPLPGVDVRIVM 405
Cdd:cd05973 195 AGAEVPARPKGRLRRVSSAGEPLtPEVIRWFDAAlGVPIHDHYGQTELGMVLANhhALEHPVHAGSAGRAMPGWRVAVLD 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 406 NNKinTSIVEGnh*ntwvRPG---LEGKEGELLvrgqsVFKEYWNKPQETresfTDDGWFKTGTepslsspafvLTHFSA 482
Cdd:cd05973 275 DDG--DELGPG-------EPGrlaIDIANSPLM-----WFRGYQLPDTPA----IDGGYYLTGD----------TVEFDP 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 483 advrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYW 562
Cdd:cd05973 327 ---------------------------------------------------------------------------DGSFS 331
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 563 IMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLT---LPELQTWAREHMGPY 639
Cdd:cd05973 332 FIGRAD-DVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTpalADELQLHVKKRLSAH 410
|
570 580
....*....|....*....|..
gi 928020699 640 KIPTGLLLVEEMPRNQMGKINK 661
Cdd:cd05973 411 AYPRTIHFVDELPKTPSGKIQR 432
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
63-664 |
2.50e-23 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 104.24 E-value: 2.50e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 63 APEFGEKLAVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKH 142
Cdd:PRK07788 59 ARRAPDRAALIDERGTLTYAELDEQSNALARGLLAL-----GVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGF 133
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 143 PPSELEYIISDSQSSLVVAGHPYEEILQPLAQKLGLPCLPLPPTNNLDALDETD-TREKEITITD------WTDRPAMII 215
Cdd:PRK07788 134 SGPQLAEVAAREGVKALVYDDEFTDLLSALPPDLGRLRAWGGNPDDDEPSGSTDeTLDDLIAGSStaplpkPPKPGGIVI 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 216 YTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCpLWVGATCVMLPDFQAQKVWEMLL 295
Cdd:PRK07788 214 LTSGTTGTPKGAPRPEPSPLAPLAGLLSRVPFRAGETTLLPAPMFHATGWAHLTLA-MALGSTVVLRRRFDPEATLEDIA 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 296 SSKAPTInvfMAVPTIYSKLIQFYDQHYQQPLLKefvkavckeRIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEI 375
Cdd:PRK07788 293 KHKATAL---VVVPVMLSRILDLGPEVLAKYDTS---------SLKIIFVSGSALSPELATRALEAFGPVLYNLYGSTEV 360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 376 GMA-LSNPLTGPRIPGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVRGQSVFKEYwnkpqetre 454
Cdd:PRK07788 361 AFAtIATPEDLAEAPGTVGRPPKGVTVKILDENG---------------NEVPRGVVGRIFVGNGFPFEGY--------- 416
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 455 sfTDDGwfktgtepslsSPAFVLTHFSAADVrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltpt 534
Cdd:PRK07788 417 --TDGR-----------DKQIIDGLLSSGDV------------------------------------------------- 434
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 535 mt*ltslqrfAHIHPlgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAV 614
Cdd:PRK07788 435 ----------GYFDE--------DGLLFVDGRDD-DMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAF 495
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|
gi 928020699 615 VQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK07788 496 VVKAPGAALDEDAIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKREL 545
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
211-653 |
3.90e-23 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 100.84 E-value: 3.90e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 211 PAMIIYTSGTTGRPKGVLHTHSSIqaMTQCLVSAW--AWSkNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQ 288
Cdd:cd17636 2 PVLAIYTAAFSGRPNGALLSHQAL--LAQALVLAVlqAID-EGTVFLNSGPLFHIGTLMFTLATFHAGGTNVFVRRVDAE 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 289 KVWEMLLSSKAptINVFMAVPTIyskliqfyDQhyqqplLKEFVKAV-----CKERIRLMVSGSAALPLPTlqrwkeitg 363
Cdd:cd17636 79 EVLELIEAERC--THAFLLPPTI--------DQ------IVELNADGlydlsSLRSSPAAPEWNDMATVDT--------- 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 364 hTLLER----YGMTEI-GMALSNPLTGPRIpGSVGVPLPGVDVRIVMNnkintsivEGNH*NTwvrpgleGKEGELLVRG 438
Cdd:cd17636 134 -SPWGRkpggYGQTEVmGLATFAALGGGAI-GGAGRPSPLVQVRILDE--------DGREVPD-------GEVGEIVARG 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 439 QSVFKEYWNKPQETRESFTDdGWFKTGtepslsspafvlthfsaadvrtliDMlpGKgvnsaycsctirrcrgtlpREEr 518
Cdd:cd17636 197 PTVMAGYWNRPEVNARRTRG-GWHHTN------------------------DL--GR-------------------REP- 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 519 cqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYWIMGrTSVDILKSGGFKISALEVECHLLAHPDIADVA 598
Cdd:cd17636 230 ---------------------------------------DGSLSFVG-PKTRMIKSGAENIYPAEVERCLRQHPAVADAA 269
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*
gi 928020699 599 VIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPR 653
Cdd:cd17636 270 VIGVPDPRWAQSVKAIVVLKPGASVTEAELIEHCRARIASYKKPKSVEFADALPR 324
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
209-664 |
5.38e-23 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 103.42 E-value: 5.38e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQA-MTQclVSAWAWSKN------DIILHTLPLHHLHGIVNKLLCPLWVGATCVM 281
Cdd:PRK08751 208 DDIAFLQYTGGTTGVAKGAMLTHRNLVAnMQQ--AHQWLAGTGkleegcEVVITALPLYHIFALTANGLVFMKIGGCNHL 285
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 282 LPDFQAQKVWEMLLssKAPTINVFMAVPTIYSKLIQF--YDQhyqqpllkefvkaVCKERIRLMVSGSAALPLPTLQRWK 359
Cdd:PRK08751 286 ISNPRDMPGFVKEL--KKTRFTAFTGVNTLFNGLLNTpgFDQ-------------IDFSSLKMTLGGGMAVQRSVAERWK 350
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 360 EITGHTLLERYGMTEIG-MALSNPLTGPRIPGSVGVPLPGVDVRIVMNNKINTSIvegnh*ntwvrpgleGKEGELLVRG 438
Cdd:PRK08751 351 QVTGLTLVEAYGLTETSpAACINPLTLKEYNGSIGLPIPSTDACIKDDAGTVLAI---------------GEIGELCIKG 415
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 439 QSVFKEYWNKPQETRESFTDDGWFKTgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreer 518
Cdd:PRK08751 416 PQVMKGYWKRPEETAKVMDADGWLHT------------------------------------------------------ 441
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 519 cqtsfspmlqtgltptmt*ltslqrfahihplGDTA-IYKDGVYWIMGRTSVDILKSgGFKISALEVECHLLAHPDIADV 597
Cdd:PRK08751 442 --------------------------------GDIArMDEQGFVYIVDRKKDMILVS-GFNVYPNEIEDVIAMMPGVLEV 488
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 928020699 598 AVIGAPDATWGQKVTAVVqLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK08751 489 AAVGVPDEKSGEIVKVVI-VKKDPALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRREL 554
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
58-664 |
5.82e-23 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 103.15 E-value: 5.82e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 58 PVFTKAPEFGEKLAVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVP 137
Cdd:PRK13383 40 LLAVTAARWPGRTAIIDDDGALSYRELQRATESLARRLTRD-----GVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVP 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 138 LYRKHPPSELEYIISDSQSSLVVAGHPYEEILqplaqklglpclplPPTNNLDALDETDTrekeITITDWTDRPA----- 212
Cdd:PRK13383 115 ISTEFRSDALAAALRAHHISTVVADNEFAERI--------------AGADDAVAVIDPAT----AGAEESGGRPAvaapg 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 213 -MIIYTSGTTGRPKGVLHTH--SSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIvNKLLCPLWVGATCVMLPDFQAQK 289
Cdd:PRK13383 177 rIVLLTSGTTGKPKGVPRAPqlRSAVGVWVTILDRTRLRTGSRISVAMPMFHGLGL-GMLMLTIALGGTVLTHRHFDAEA 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 290 VwemLLSSKAPTINVFMAVPTIYSKLIQFYDQ-HYQQPLlkefvkavckERIRLMVSGSAALPlPTL-QRWKEITGHTLL 367
Cdd:PRK13383 256 A---LAQASLHRADAFTAVPVVLARILELPPRvRARNPL----------PQLRVVMSSGDRLD-PTLgQRFMDTYGDILY 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 368 ERYGMTEIGM-ALSNPLTGPRIPGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVRGQsvfkeyw 446
Cdd:PRK13383 322 NGYGSTEVGIgALATPADLRDAPETVGKPVAGCPVRILDRNN---------------RPVGPRVTGRIFVGGE------- 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 447 nkpqetresftddgwfktgtepsLSSpafvlTHFSAADVRTLIDmlpgkgvnsaycsctirrcrgtlpreercqtsfspm 526
Cdd:PRK13383 380 -----------------------LAG-----TRYTDGGGKAVVD------------------------------------ 395
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 527 lqtGLTPTmt*ltslqrfahihplGDTAiYKD--GVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPD 604
Cdd:PRK13383 396 ---GMTST----------------GDMG-YLDnaGRLFIVGRED-DMIISGGENVYPRAVENALAAHPAVADNAVIGVPD 454
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 605 ATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK13383 455 ERFGHRLAAFVVLHPGSGVDAAQLRDYLKDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
216-661 |
8.37e-22 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 99.64 E-value: 8.37e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 216 YTSGTTGRPKGVLHTH--SSIQAMTQCLvsAWAWSKNDIIlhtlplhhlhgivnkllcpLWV------------------ 275
Cdd:PRK08162 189 YTSGTTGNPKGVVYHHrgAYLNALSNIL--AWGMPKHPVY-------------------LWTlpmfhcngwcfpwtvaar 247
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 276 GATCVMLPDFQAQKVWEMLLSSKaptINVFMAVPTIYSKLIQFYDQHyqqpllkefvKAVCKERIRLMVSGsAALPLPTL 355
Cdd:PRK08162 248 AGTNVCLRKVDPKLIFDLIREHG---VTHYCGAPIVLSALINAPAEW----------RAGIDHPVHAMVAG-AAPPAAVI 313
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 356 QRWKEItGHTLLERYGMTEI-GMALSN---------PLTG-PRIPGSVGVPLPGVDVRIVMNNKINTSIvegnh*ntwvr 424
Cdd:PRK08162 314 AKMEEI-GFDLTHVYGLTETyGPATVCawqpewdalPLDErAQLKARQGVRYPLQEGVTVLDPDTMQPV----------- 381
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 425 PGlEGKE-GELLVRGQSVFKEYWNKPQETRESFTDdGWFKTGtepslsspafvlthfsaadvrtliDMlpgkGVnsaycs 503
Cdd:PRK08162 382 PA-DGETiGEIMFRGNIVMKGYLKNPKATEEAFAG-GWFHTG------------------------DL----AV------ 425
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 504 ctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahIHPlgdtaiykDGVYWIMGRtSVDILKSGGFKISALE 583
Cdd:PRK08162 426 -------------------------------------------LHP--------DGYIKIKDR-SKDIIISGGENISSIE 453
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 928020699 584 VECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLvEEMPRNQMGKINK 661
Cdd:PRK08162 454 VEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASATEEEIIAHCREHLAGFKVPKAVVF-GELPKTSTGKIQK 530
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
103-665 |
1.89e-21 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 97.83 E-value: 1.89e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 103 FGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQPLAqklglpclp 182
Cdd:cd05929 37 EGVWIADGVYIYLINSILTVFAAAAAWKCGACPAYKSSRAPRAEACAIIEIKAAALVCGLFTGGGALDGLE--------- 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 183 lpptnnldALDETDTREKEITITDwTDRPAMIIYTSGTTGRPKGVL--HTHSSIQAMTQCLVSAWAWSKNDIIlhtlplh 260
Cdd:cd05929 108 --------DYEAAEGGSPETPIED-EAAGWKMLYSGGTTGRPKGIKrgLPGGPPDNDTLMAAALGFGPGADSV------- 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 261 hlhgivnkLLCP---------------LWVGATCVMLPDFQAQkvwEMLLSSKAPTINVFMAVPTIYSKLIQFYDQ---H 322
Cdd:cd05929 172 --------YLSPaplyhaapfrwsmtaLFMGGTLVLMEKFDPE---EFLRLIERYRVTFAQFVPTMFVRLLKLPEAvrnA 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 323 YQQPLLKEFVKAvckerirlmvsgSAALPLPTLQRWKEITGHTLLERYGMTE-IGMALSNP---LTGPripGSVGVPLPG 398
Cdd:cd05929 241 YDLSSLKRVIHA------------AAPCPPWVKEQWIDWGGPIIWEYYGGTEgQGLTIINGeewLTHP---GSVGRAVLG 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 399 vDVRIVMNNkintsiveGNH*NTwvrpgleGKEGELLVRGQSVFkEYWNKPQETRESFTDDGWfktgtepslsspafvlt 478
Cdd:cd05929 306 -KVHILDED--------GNEVPP-------GEIGEVYFANGPGF-EYTNDPEKTAAARNEGGW----------------- 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 479 hfsaadvRTLIDMlpgkgvnsaycsctirrcrGTLpreercqtsfspmlqtgltptmt*ltslqrfahihplgDTaiykD 558
Cdd:cd05929 352 -------STLGDV-------------------GYL--------------------------------------DE----D 363
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 559 GVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKG-KSLTLP--ELQTWAREH 635
Cdd:cd05929 364 GYLYLTDRRS-DMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVQPAPGaDAGTALaeELIAFLRDR 442
|
570 580 590
....*....|....*....|....*....|
gi 928020699 636 MGPYKIPTGLLLVEEMPRNQMGKINKKDLL 665
Cdd:cd05929 443 LSRYKCPRSIEFVAELPRDDTGKLYRRLLR 472
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
137-661 |
1.89e-21 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 98.73 E-value: 1.89e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 137 PLYRkhpPSELEYIISDSQSSLVVA-----GHPYEEILQPLAQKLGLPCLPLPPTNNLDAL------------------- 192
Cdd:PRK08315 100 PAYR---LSELEYALNQSGCKALIAadgfkDSDYVAMLYELAPELATCEPGQLQSARLPELrrviflgdekhpgmlnfde 176
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 193 ----------DETDTREKEITITDwtdrPAMIIYTSGTTGRPKGVLHTHSSIQ------AMTQCLvsawawSKNDiilht 256
Cdd:PRK08315 177 llalgravddAELAARQATLDPDD----PINIQYTSGTTGFPKGATLTHRNILnngyfiGEAMKL------TEED----- 241
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 257 lplhhlhgivnKLLCP----------------LWVGATCV-MLPDFQAQKVWEMLLSSKA------PTInvFMAVptiys 313
Cdd:PRK08315 242 -----------RLCIPvplyhcfgmvlgnlacVTHGATMVyPGEGFDPLATLAAVEEERCtalygvPTM--FIAE----- 303
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 314 kliqfydqhYQQPLLKEFvkAVCKERIRLMvSGSAAlPLPTLQR------WKEITghtllERYGMTEigmalSNPL---T 384
Cdd:PRK08315 304 ---------LDHPDFARF--DLSSLRTGIM-AGSPC-PIEVMKRvidkmhMSEVT-----IAYGMTE-----TSPVstqT 360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 385 GPRIP-----GSVGVPLPGVDVRIVMNNKINTsivegnh*ntwVRPGlegKEGELLVRGQSVFKEYWNKPQETRESFTDD 459
Cdd:PRK08315 361 RTDDPlekrvTTVGRALPHLEVKIVDPETGET-----------VPRG---EQGELCTRGYSVMKGYWNDPEKTAEAIDAD 426
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 460 GWFKTGtepslsspafvlthfsaaDVRTLIDmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*lt 539
Cdd:PRK08315 427 GWMHTG------------------DLAVMDE------------------------------------------------- 439
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 540 slqrfahihplgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRK 619
Cdd:PRK08315 440 ------------------EGYVNIVGRIK-DMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRP 500
|
570 580 590 600
....*....|....*....|....*....|....*....|..
gi 928020699 620 GKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:PRK08315 501 GATLTEEDVRDFCRGKIAHYKIPRYIRFVDEFPMTVTGKIQK 542
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
109-659 |
2.81e-21 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 97.54 E-value: 2.81e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 109 KRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYeeilqplaqklglpclplpptnn 188
Cdd:PRK07638 51 KTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDELKERLAISNADMIVTERYK----------------------- 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 189 LDALDETDTREKEI-----TITDWTDRPAMII----------YTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDII 253
Cdd:PRK07638 108 LNDLPDEEGRVIEIdewkrMIEKYLPTYAPIEnvqnapfymgFTSGSTGKPKAFLRAQQSWLHSFDCNVHDFHMKREDSV 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 254 LHTLPLHHLH---GIVNKLlcplWVGATCVMLPDFQAQKVWEMLlssKAPTINVFMAVPTIYskliqfydqhyqQPLLKE 330
Cdd:PRK07638 188 LIAGTLVHSLflyGAISTL----YVGQTVHLMRKFIPNQVLDKL---ETENISVMYTVPTML------------ESLYKE 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 331 fvKAVCKERIRLMVSGsAALPLPTLQRWKEITGH-TLLERYGMTEIG-MALSNPLTGPRIPGSVGVPLPGVDVRIVmnnk 408
Cdd:PRK07638 249 --NRVIENKMKIISSG-AKWEAEAKEKIKNIFPYaKLYEFYGASELSfVTALVDEESERRPNSVGRPFHNVQVRIC---- 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 409 intsivegNH*NTWVRPGLEGKegeLLVRGQSVFKEYWNKPQETREsFTDDGWFktgtepslsspafvlthfsaadvrTL 488
Cdd:PRK07638 322 --------NEAGEEVQKGEIGT---VYVKSPQFFMGYIIGGVLARE-LNADGWM------------------------TV 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 489 IDMlpgkgvnsAYcsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaIYKDGVYWIMGRTS 568
Cdd:PRK07638 366 RDV--------GY-----------------------------------------------------EDEEGFIYIVGREK 384
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 569 VDILkSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVqlrKGkSLTLPELQTWAREHMGPYKIPTGLLLV 648
Cdd:PRK07638 385 NMIL-FGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAII---KG-SATKQQLKSFCLQRLSSFKIPKEWHFV 459
|
570
....*....|.
gi 928020699 649 EEMPRNQMGKI 659
Cdd:PRK07638 460 DEIPYTNSGKI 470
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
17-666 |
3.97e-21 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 99.26 E-value: 3.97e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 17 SRWKATPYRtlfYSRQRLLEATVrrnSHRSALAPswvekqkpvftkapefgEKLAVIDSCGKHSYKQLYCSslglASRIS 96
Cdd:PRK12316 1990 ADWDRTPEA---YPRGPGVHQRI---AEQAARAP-----------------EAIAVVFGDQHLSYAELDSR----ANRLA 2042
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 97 SALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILqPLAQkl 176
Cdd:PRK12316 2043 HRL-RARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLLTQRHLLERL-PLPA-- 2118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 177 glpclplpptnNLDALDETDTREkeititdWTDRP-------------AMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVS 243
Cdd:PRK12316 2119 -----------GVARLPLDRDAE-------WADYPdtapavqlagenlAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGE 2180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 244 AWAWSKNDIIlHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQ---AQKVWEMllSSKAPTINVFMAVptiyskliqfyd 320
Cdd:PRK12316 2181 RYELSPADCE-LQFMSFSFDGAHEQWFHPLLNGARVLIRDDELwdpEQLYDEM--ERHGVTILDFPPV------------ 2245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 321 qhYQQPLLKEFVKAVCKERIRLMVSGSAALPLPTLQRWKE-ITGHTLLERYGMTEigmALSNPL---TGPRIP-GSVGVP 395
Cdd:PRK12316 2246 --YLQQLAEHAERDGRPPAVRVYCFGGEAVPAASLRLAWEaLRPVYLFNGYGPTE---AVVTPLlwkCRPQDPcGAAYVP 2320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 396 lpgvdvrivmnnkINTSIveGNH*NTWVRPGLE----GKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtepsls 471
Cdd:PRK12316 2321 -------------IGRAL--GNRRAYILDADLNllapGMAGELYLGGEGLARGYLNRPGLTAERFVPDPFSASG------ 2379
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 472 spafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrGTLPREercqtsfspmlqtgltptmt*ltslqrfahihplG 551
Cdd:PRK12316 2380 ---------------------------------------ERLYRT----------------------------------G 2386
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 552 DTAIYK-DGVYWIMGRTSVDIlKSGGFKISALEVECHLLAHPDIADVAVIgAPDATWGQKVTAVVQLRKGKSLTLPELQT 630
Cdd:PRK12316 2387 DLARYRaDGVVEYLGRIDHQV-KIRGFRIELGEIEARLQAHPAVREAVVV-AQDGASGKQLVAYVVPDDAAEDLLAELRA 2464
|
650 660 670
....*....|....*....|....*....|....*.
gi 928020699 631 WAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLK 666
Cdd:PRK12316 2465 WLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPK 2500
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
68-664 |
6.32e-21 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 96.57 E-value: 6.32e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLycssLGLASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSEL 147
Cdd:cd12114 2 DATAVICGDGTLTYGEL----AERARRVAGAL-KAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARR 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 148 EYIISDSQSSLVVAGHPYEEILQPLAQklglpclplpptnNLDALDETDTREKEITITDWT-DRPAMIIYTSGTTGRPKG 226
Cdd:cd12114 77 EAILADAGARLVLTDGPDAQLDVAVFD-------------VLILDLDALAAPAPPPPVDVApDDLAYVIFTSGSTGTPKG 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 227 VLHTHSS----IQAMTQclvsAWAWSKNDIILhtlplhhlhGI--------VNKLLCPLWVGATCVMLPDFQAQKV--WE 292
Cdd:cd12114 144 VMISHRAalntILDINR----RFAVGPDDRVL---------ALsslsfdlsVYDIFGALSAGATLVLPDEARRRDPahWA 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 293 MLLSSKAPTINVFmaVPTIYSKLIQFYDQHYQQPllkefvkavckERIRL-MVSGS-AALPLPtlQRWKEITGH-TLLER 369
Cdd:cd12114 211 ELIERHGVTLWNS--VPALLEMLLDVLEAAQALL-----------PSLRLvLLSGDwIPLDLP--ARLRALAPDaRLISL 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 370 YGMTEiGMALSN--PLT-GPRIPGSV--GVPLPGVDVRIVMNNkintsiveGNH*NTWVRpglegkeGELLVRGQSVFKE 444
Cdd:cd12114 276 GGATE-ASIWSIyhPIDeVPPDWRSIpyGRPLANQRYRVLDPR--------GRDCPDWVP-------GELWIGGRGVALG 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 445 YWNKPQETRESFTDDG----WFKTgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercq 520
Cdd:cd12114 340 YLGDPELTAARFVTHPdgerLYRT-------------------------------------------------------- 363
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 521 tsfspmlqtgltptmt*ltslqrfahihplGDTAIYK-DGVYWIMGRtsVDI-LKSGGFKISALEVECHLLAHPDIADVA 598
Cdd:cd12114 364 ------------------------------GDLGRYRpDGTLEFLGR--RDGqVKVRGYRIELGEIEAALQAHPGVARAV 411
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 928020699 599 VIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd12114 412 VVVLGDPGGKRLAAFVVPDNDGTPIAPDALRAFLAQTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
71-669 |
8.07e-21 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 96.83 E-value: 8.07e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 71 AVIDSCGKH--SYKQLycssLGLASRISSALGSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELE 148
Cdd:PLN02574 57 ALIDSSTGFsiSYSEL----QPLVKSMAAGLYHVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIK 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 149 YIISDSQSSLVVAGHPYEEILQPLAqklgLPCLPLPPTNNLDALDETDTREKEITITDW---------TDRPAMIIYTSG 219
Cdd:PLN02574 133 KRVVDCSVGLAFTSPENVEKLSPLG----VPVIGVPENYDFDSKRIEFPKFYELIKEDFdfvpkpvikQDDVAAIMYSSG 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 220 TTGRPKGVLHTHSSIQAMTQCLV----SAWAWSKND-IILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKVWEML 294
Cdd:PLN02574 209 TTGASKGVVLTHRNLIAMVELFVrfeaSQYEYPGSDnVYLAALPMFHIYGLSLFVVGLLSLGSTIVVMRRFDASDMVKVI 288
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 295 LSSKaptINVFMAVPTIYSKLIqfydqHYQQPllkefVKAVCKERIRLMVSGSAALPLPTLQRWKEITGHT-LLERYGMT 373
Cdd:PLN02574 289 DRFK---VTHFPVVPPILMALT-----KKAKG-----VCGEVLKSLKQVSCGAAPLSGKFIQDFVQTLPHVdFIQGYGMT 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 374 EIGMALSNPLTGPRIP--GSVGVPLPGVDVRIVmnnkintsivegnh*nTWVRPGL--EGKEGELLVRGQSVFKEYWNKP 449
Cdd:PLN02574 356 ESTAVGTRGFNTEKLSkySSVGLLAPNMQAKVV----------------DWSTGCLlpPGNCGELWIQGPGVMKGYLNNP 419
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 450 QETRESFTDDGWFKTGtepslsspafvlthfsaaDVrtlidmlpgkgvnsAYcsctirrcrgtlpreercqtsfspmlqt 529
Cdd:PLN02574 420 KATQSTIDKDGWLRTG------------------DI--------------AY---------------------------- 439
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 530 gltptmt*ltslqrFAHihplgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQ 609
Cdd:PLN02574 440 --------------FDE-----------DGYLYIVDRLK-EIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGE 493
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 610 KVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKdLLKHFF 669
Cdd:PLN02574 494 IPVAFVVRRQGSTLSQEAVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRR-ELKRSL 552
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
209-465 |
1.01e-20 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 96.49 E-value: 1.01e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTH----SSIQAMTQC----------LVSaWA-WSK----NDIIlhtlplhhlhGIVnkl 269
Cdd:PRK08180 209 DTIAKFLFTSGSTGLPKAVINTHrmlcANQQMLAQTfpflaeeppvLVD-WLpWNHtfggNHNL----------GIV--- 274
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 270 lcpLWVGATcVMLPDFQ--AQKVWEML--LSSKAPTinVFMAVPTIYSKLIQFYDQhyQQPLLKEFVkavckERIRLMVS 345
Cdd:PRK08180 275 ---LYNGGT-LYIDDGKptPGGFDETLrnLREISPT--VYFNVPKGWEMLVPALER--DAALRRRFF-----SRLKLLFY 341
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 346 GSAALPLPTLQRWKEITGHTLLER------YGMTEIGMALSNpLTGPRI-PGSVGVPLPGVDVRIVMNNkintsivegnh 418
Cdd:PRK08180 342 AGAALSQDVWDRLDRVAEATCGERirmmtgLGMTETAPSATF-TTGPLSrAGNIGLPAPGCEVKLVPVG----------- 409
|
250 260 270 280
....*....|....*....|....*....|....*....|....*..
gi 928020699 419 *ntwvrpgleGKEgELLVRGQSVFKEYWNKPQETRESFTDDGWFKTG 465
Cdd:PRK08180 410 ----------GKL-EVRVKGPNVTPGYWRAPELTAEAFDEEGYYRSG 445
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
92-668 |
1.25e-20 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 96.01 E-value: 1.25e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 92 ASRISSALGSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQP 171
Cdd:PRK05620 48 AAALAHALHDELGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDEVIVADPRLAEQLGE 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 172 L-----------------AQKLGLPCLPLPPTNNLDALdeTDTREkeiTITDWTD----RPAMIIYTSGTTGRPKGVLHT 230
Cdd:PRK05620 128 IlkecpcvravvfigpsdADSAAAHMPEGIKVYSYEAL--LDGRS---TVYDWPEldetTAAAICYSTGTTGAPKGVVYS 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 231 HSSIQAMTQCLVSAWAWS-KNDiilhtlplhhlhgiVNKLLC-PLW-VGATCVMLPDFQAQKVWEMLLSS-KAPTINVFM 306
Cdd:PRK05620 203 HRSLYLQSLSLRTTDSLAvTHG--------------ESFLCCvPIYhVLSWGVPLAAFMSGTPLVFPGPDlSAPTLAKII 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 307 A---------VPTIYSKLIQFYDQHyqQPllkefvkavckERIRL--MVSGSAALPLPTLQRWKEITGHTLLERYGMTE- 374
Cdd:PRK05620 269 AtamprvahgVPTLWIQLMVHYLKN--PP-----------ERMSLqeIYVGGSAVPPILIKAWEERYGVDVVHVWGMTEt 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 375 --IGmALSNPLTGP--------RIpgSVGVPLPGVDVRIVMNNKIntsiVEGNH*NtwvrpglegkEGELLVRGQSVFKE 444
Cdd:PRK05620 336 spVG-TVARPPSGVsgearwayRV--SQGRFPASLEYRIVNDGQV----MESTDRN----------EGEIQVRGNWVTAS 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 445 YWNKPQET----------------RESFTDDGWFKTGTEPSLSSPAFVLTHFSAADVrtlidmlpgkgvnsaycsctIRr 508
Cdd:PRK05620 399 YYHSPTEEgggaastfrgedvedaNDRFTADGWLRTGDVGSVTRDGFLTIHDRARDV--------------------IR- 457
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 509 crgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykdgvywimgrtsvdilkSGGFKISALEVECHL 588
Cdd:PRK05620 458 -----------------------------------------------------------------SGGEWIYSAQLENYI 472
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 589 LAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSL---TLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLL 665
Cdd:PRK05620 473 MAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPtreTAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDLR 552
|
...
gi 928020699 666 KHF 668
Cdd:PRK05620 553 QHL 555
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
202-465 |
1.39e-20 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 96.58 E-value: 1.39e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 202 ITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAmtqclvsawawskndiilhtlplhhlhGI------VNKLLCPLWV 275
Cdd:PTZ00216 257 LNIPENNDDLALIMYTSGTTGDPKGVMHTHGSLTA---------------------------GIlaledrLNDLIGPPEE 309
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 276 GAT-CVMLP-----DFQAQKVWEM------------LLSSKA----------PTinVFMAVPTIY--------SKLI--- 316
Cdd:PTZ00216 310 DETyCSYLPlahimEFGVTNIFLArgaligfgsprtLTDTFArphgdltefrPV--FLIGVPRIFdtikkaveAKLPpvg 387
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 317 ----QFYDQHYQQPL--LKE-----------F--VKAVCKERIRLMVSGSAALPLPTlQRWKEITGHTLLERYGMTEIGM 377
Cdd:PTZ00216 388 slkrRVFDHAYQSRLraLKEgkdtpywnekvFsaPRAVLGGRVRAMLSGGGPLSAAT-QEFVNVVFGMVIQGWGLTETVC 466
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 378 ALSNPLTGPRIPGSVGVPLPGVDVRIVmnnkintSIVEGNH*NTwVRPglegkEGELLVRGQSVFKEYWNKPQETRESFT 457
Cdd:PTZ00216 467 CGGIQRTGDLEPNAVGQLLKGVEMKLL-------DTEEYKHTDT-PEP-----RGEILLRGPFLFKGYYKQEELTREVLD 533
|
....*...
gi 928020699 458 DDGWFKTG 465
Cdd:PTZ00216 534 EDGWFHTG 541
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
79-465 |
3.45e-20 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 94.61 E-value: 3.45e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 79 HSYKQLYCSSLGLASRISSAlgsdfgGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSE---LEYIISDSQ 155
Cdd:cd05931 25 LTYAELDRRARAIAARLQAV------GKPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLPPPTPGRHaerLAAILADAG 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 156 SSLVVAGHPYEEILQPLAQKLGLPCLPLPPTnnLDALDETDTRekeititDWT------DRPAMIIYTSGTTGRPKGVLH 229
Cdd:cd05931 99 PRVVLTTAAALAAVRAFAASRPAAGTPRLLV--VDLLPDTSAA-------DWPppspdpDDIAYLQYTSGSTGTPKGVVV 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 230 THSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLP--DFQAQKV-WEMLLSSKAPTInvfM 306
Cdd:cd05931 170 THRNLLANVRQIRRAYGLDPGDVVVSWLPLYHDMGLIGGLLTPLYSGGPSVLMSpaAFLRRPLrWLRLISRYRATI---S 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 307 AVPTiyskliqF-YDQhyqqpllkefvkavCKERIR-----------LMVSGSAALPL--PTLQRWKE------ITGHTL 366
Cdd:cd05931 247 AAPN-------FaYDL--------------CVRRVRdedlegldlssWRVALNGAEPVrpATLRRFAEafapfgFRPEAF 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 367 LERYGMTE-------------------IGMALSNPLTGPRIPG-------SVGVPLPGVDVRIVmnnkintsivegnH*N 420
Cdd:cd05931 306 RPSYGLAEatlfvsggppgtgpvvlrvDRDALAGRAVAVAADDpaarelvSCGRPLPDQEVRIV-------------DPE 372
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|.
gi 928020699 421 TwVRPGLEGKEGELLVRGQSVFKEYWNKPQETRESF------TDDGWFKTG 465
Cdd:cd05931 373 T-GRELPDGEVGEIWVRGPSVASGYWGRPEATAETFgalaatDEGGWLRTG 422
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
68-465 |
4.49e-20 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 93.53 E-value: 4.49e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLASRISsalgsDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSEL 147
Cdd:cd17653 12 DAVAVESLGGSLTYGELDAASNALANRLL-----QLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAKLPSARI 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 148 EYIISDSQSSLVVAghpyeeilqplaqklglpclplpptnnldaldetdtrekeitiTDWTDRPAMIIYTSGTTGRPKGV 227
Cdd:cd17653 87 QAILRTSGATLLLT-------------------------------------------TDSPDDLAYIIFTSGSTGIPKGV 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 228 LHTHSSIQAMTQ-----------------CLVSAWAwskndiilhtlplhhlhgIVNKLLCPLWVGATCV---MLPDFQa 287
Cdd:cd17653 124 MVPHRGVLNYVSqpparldvgpgsrvaqvLSIAFDA------------------CIGEIFSTLCNGGTLVladPSDPFA- 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 288 qkvwemllsSKAPTINVFMAVPTIYSKLiqfYDQHYQqpllkefvkavckeRIRLMVSGSAALPLPTLQRWKEitGHTLL 367
Cdd:cd17653 185 ---------HVARTVDALMSTPSILSTL---SPQDFP--------------NLKTIFLGGEAVPPSLLDRWSP--GRRLY 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 368 ERYGMTEIGMALSNPLTGPRIPGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVRGQSVFKEYWN 447
Cdd:cd17653 237 NAYGPTECTISSTMTELLPGQPVTIGKPIPNSTCYILDADL---------------QPVPEGVVGEICISGVQVARGYLG 301
|
410 420
....*....|....*....|....
gi 928020699 448 KPQETRESFTDDGW------FKTG 465
Cdd:cd17653 302 NPALTASKFVPDPFwpgsrmYRTG 325
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
211-659 |
4.75e-20 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 94.44 E-value: 4.75e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 211 PAMIIYTSGTTGRPKGVLHThsSIQAMTQCLVSAWA----------WSKNDIilhtlplhhlhG-------IVnklLCPL 273
Cdd:PRK00174 247 PLFILYTSGSTGKPKGVLHT--TGGYLVYAAMTMKYvfdykdgdvyWCTADV-----------GwvtghsyIV---YGPL 310
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 274 WVGATCVML---PDF-QAQKVWEMLLSSKaptINVFMAVPTIYSKLIQFYDQHYQQPLLKEfvkavckerIRLMvsGSAA 349
Cdd:PRK00174 311 ANGATTLMFegvPNYpDPGRFWEVIDKHK---VTIFYTAPTAIRALMKEGDEHPKKYDLSS---------LRLL--GSVG 376
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 350 LPL-PTLQRW-KEITGHtllER------YGMTEIGMALSNPLTG--PRIPGSVGVPLPGVDVRIVMNnkintsivEGNH* 419
Cdd:PRK00174 377 EPInPEAWEWyYKVVGG---ERcpivdtWWQTETGGIMITPLPGatPLKPGSATRPLPGIQPAVVDE--------EGNP- 444
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 420 ntwvrpgLEGKEGELLVrgqsvFKEYWnkPQETRESFTDDGwfktgtepslsspAFVLTHFSAadvrtlidmLPGKgvns 499
Cdd:PRK00174 445 -------LEGGEGGNLV-----IKDPW--PGMMRTIYGDHE-------------RFVKTYFST---------FKGM---- 484
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 500 aYcsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrFAhihplGDTAIY-KDGVYWIMGRTSvDILKSGGFK 578
Cdd:PRK00174 485 -Y------------------------------------------FT-----GDGARRdEDGYYWITGRVD-DVLNVSGHR 515
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 579 ISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLP---ELQTWAREHMGPYKIPTGLLLVEEMPRNQ 655
Cdd:PRK00174 516 LGTAEIESALVAHPKVAEAAVVGRPDDIKGQGIYAFVTLKGGEEPSDElrkELRNWVRKEIGPIAKPDVIQFAPGLPKTR 595
|
....
gi 928020699 656 MGKI 659
Cdd:PRK00174 596 SGKI 599
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
68-664 |
6.14e-20 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 93.15 E-value: 6.14e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSEL 147
Cdd:cd12115 14 DAIALVCGDESLTYAELNRRANRLAARLRAA-----GVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPAYPPERL 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 148 EYIISDSQSSLVvaghpyeeilqplaqklglpclplpptnnldaldetdtrekeitITDWTDrPAMIIYTSGTTGRPKGV 227
Cdd:cd12115 89 RFILEDAQARLV--------------------------------------------LTDPDD-LAYVIYTSGSTGRPKGV 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 228 LHTHSSIQAMTQclvsawaWSKNDIilhtlPLHHLHGI-----------VNKLLCPLWVGATCVMlpdfqAQKVWEMLLS 296
Cdd:cd12115 124 AIEHRNAAAFLQ-------WAAAAF-----SAEELAGVlastsicfdlsVFELFGPLATGGKVVL-----ADNVLALPDL 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 297 SKAPTINVFMAVPTIYSKLIqfydqhyQQPLLKEFVKAVCkerirlmVSGSaALPLPTLQR-WKEITGHTLLERYGMTE- 374
Cdd:cd12115 187 PAAAEVTLINTVPSAAAELL-------RHDALPASVRVVN-------LAGE-PLPRDLVQRlYARLQVERVVNLYGPSEd 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 375 ----IGMALSnplTGPRIPGSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVRGQSVFKEYWNKPQ 450
Cdd:cd12115 252 ttysTVAPVP---PGASGEVSIGRPLANTQAYVLDRAL---------------QPVPLGVPGELYIGGAGVARGYLGRPG 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 451 ETRESFTDDGWFktgtepslsspafvlthfsaadvrtlidmlPGkgvnsaycsctiRRCRGTlpreercqtsfspmlqtg 530
Cdd:cd12115 314 LTAERFLPDPFG------------------------------PG------------ARLYRT------------------ 333
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 531 ltptmt*ltslqrfahihplGDTAIYK-DGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQ 609
Cdd:cd12115 334 --------------------GDLVRWRpDGLLEFLGRAD-NQVKVRGFRIELGEIEAALRSIPGVREAVVVAIGDAAGER 392
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*
gi 928020699 610 KVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd12115 393 RLVAYIVAEPGAAGLVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
122-664 |
1.27e-19 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 94.25 E-value: 1.27e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVvaghpyeeilqpLAQKLGLPCLPLPPTNNLDALDETDTREKE 201
Cdd:PRK12316 3121 VVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLL------------LSQSHLRLPLAQGVQVLDLDRGDENYAEAN 3188
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 202 ITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIIlHTLPLHHLHGIVNKLLCPLWVGATCVM 281
Cdd:PRK12316 3189 PAIRTMPENLAYVIYTSGSTGKPKGVGIRHSALSNHLCWMQQAYGLGVGDRV-LQFTTFSFDVFVEELFWPLMSGARVVL 3267
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 282 LPDFQAQKVWEMLLSSKAPTINVFMAVPTIYSKLIQFYDQHYQQPllkefvkavckerIRLMVSGSAALPLPTLQRWkeI 361
Cdd:PRK12316 3268 AGPEDWRDPALLVELINSEGVDVLHAYPSMLQAFLEEEDAHRCTS-------------LKRIVCGGEALPADLQQQV--F 3332
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 362 TGHTLLERYGMTEIGMALSNPLTGPRIPGS--VGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGKEGELLVRGQ 439
Cdd:PRK12316 3333 AGLPLYNLYGPTEATITVTHWQCVEEGKDAvpIGRPIANRACYIL---------------DGSLEPVPVGALGELYLGGE 3397
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 440 SVFKEYWNKPQETRESFTDDgwfktgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreerc 519
Cdd:PRK12316 3398 GLARGYHNRPGLTAERFVPD------------------------------------------------------------ 3417
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 520 qtSFSPMlqtgltptmt*ltslqrfAHIHPLGDTAIYK-DGVYWIMGRTSVDIlKSGGFKISALEVECHLLAHPDIADVA 598
Cdd:PRK12316 3418 --PFVPG------------------ERLYRTGDLARYRaDGVIEYIGRVDHQV-KIRGFRIELGEIEARLLEHPWVREAV 3476
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 928020699 599 VIgapdATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK12316 3477 VL----AVDGRQLVAYVVPEDEAGDLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKAL 3538
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
583-658 |
1.30e-19 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 83.36 E-value: 1.30e-19
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 928020699 583 EVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGK 658
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
59-664 |
1.72e-19 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 92.35 E-value: 1.72e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 59 VFTKAPEFGEKLAVID-SCGK-HSYKQLYCSSlglaSRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGG--- 133
Cdd:PLN02246 29 CFERLSEFSDRPCLIDgATGRvYTYADVELLS----RRVAAGL-HKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAvtt 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 134 TAVPLYrkhPPSELEYIISDSQSSLVVAGHPYEEILQPLAQKLGLPCLPL-------PPTNNLDALDETDTREKEITitd 206
Cdd:PLN02246 104 TANPFY---TPAEIAKQAKASGAKLIITQSCYVDKLKGLAEDDGVTVVTIddppegcLHFSELTQADENELPEVEIS--- 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 207 wTDRPAMIIYTSGTTGRPKGVLHTHSSiqamtqcLVSAWA-----------WSKNDIILHTLPLHHLHGIVNKLLCPLWV 275
Cdd:PLN02246 178 -PDDVVALPYSSGTTGLPKGVMLTHKG-------LVTSVAqqvdgenpnlyFHSDDVILCVLPMFHIYSLNSVLLCGLRV 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 276 GATCVMLPDFQAQKVWEMLLSSKaptINVFMAVPtiyskliqfydqhyqqPLLKEFVKAVCKER-----IRLMVSGSAAL 350
Cdd:PLN02246 250 GAAILIMPKFEIGALLELIQRHK---VTIAPFVP----------------PIVLAIAKSPVVEKydlssIRMVLSGAAPL 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 351 --PLPTLQRWKeITGHTLLERYGMTEIGMALS-------NPLtgPRIPGSVGVPLPGVDVRIVmnnkintsivegnH*NT 421
Cdd:PLN02246 311 gkELEDAFRAK-LPNAVLGQGYGMTEAGPVLAmclafakEPF--PVKSGSCGTVVRNAELKIV-------------DPET 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 422 WVRPGlEGKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaaDVrTLIDmlpgkgvnsay 501
Cdd:PLN02246 375 GASLP-RNQPGEICIRGPQIMKGYLNDPEATANTIDKDGWLHTG------------------DI-GYID----------- 423
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 502 csctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplGDTAIYkdgvywIMGRTSvDILKSGGFKISA 581
Cdd:PLN02246 424 -------------------------------------------------DDDELF------IVDRLK-ELIKYKGFQVAP 447
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 582 LEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:PLN02246 448 AELEALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGSEITEDEIKQFVAKQVVFYKRIHKVFFVDSIPKAPSGKILR 527
|
...
gi 928020699 662 KDL 664
Cdd:PLN02246 528 KDL 530
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
212-664 |
3.96e-19 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 91.05 E-value: 3.96e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 212 AMIIYTSGTTGRPKGVLHTHSSIQA-MTQCLVSAWAWSKNDIILHTLPLHHLHGI-VNKLLCPLWVGATCVMLPDFQAqk 289
Cdd:cd17642 187 ALIMNSSGSTGLPKGVQLTHKNIVArFSHARDPIFGNQIIPDTAILTVIPFHHGFgMFTTLGYLICGFRVVLMYKFEE-- 264
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 290 vwEMLLSS--KAPTINVFMaVPTIYSkliqFYDQHyqqPLLKEFvkavckERIRLMVSGSAALPLPtlqrwKEItGHTLL 367
Cdd:cd17642 265 --ELFLRSlqDYKVQSALL-VPTLFA----FFAKS---TLVDKY------DLSNLHEIASGGAPLS-----KEV-GEAVA 322
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 368 ER---------YGMTEIGMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnKINTSIVEGNH*ntwvrpglegKEGELLVRG 438
Cdd:cd17642 323 KRfklpgirqgYGLTETTSAILITPEGDDKPGAVGKVVPFFYAKVV---DLDTGKTLGPN-----------ERGELCVKG 388
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 439 QSVFKEYWNKPQETRESFTDDGWFKTgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreer 518
Cdd:cd17642 389 PMIMKGYVNNPEATKALIDKDGWLHS------------------------------------------------------ 414
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 519 cqtsfspmlqtgltptmt*ltslqrfahihplGDTAIY-KDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADV 597
Cdd:cd17642 415 --------------------------------GDIAYYdEDGHFFIVDRLK-SLIKYKGYQVPPAELESILLQHPKIFDA 461
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 928020699 598 AVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYK-IPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd17642 462 GVAGIPDEDAGELPAAVVVLEAGKTMTEKEVMDYVASQVSTAKrLRGGVKFVDEVPKGLTGKIDRRKI 529
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
68-664 |
5.95e-19 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 90.19 E-value: 5.95e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLASRISSaLGSDFGGLDGkrisfLCANDS-SYVVAQWAVWMSGGTAVPLYRKHPPSE 146
Cdd:cd17644 15 DAVAVVFEDQQLTYEELNTKANQLAHYLQS-LGVKSESLVG-----ICVERSlEMIIGLLAILKAGGAYVPLDPNYPQER 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 147 LEYIISDSQSSLVVAghpyeeilQPlaqklglpclplpptNNLdaldetdtrekeititdwtdrpAMIIYTSGTTGRPKG 226
Cdd:cd17644 89 LTYILEDAQISVLLT--------QP---------------ENL----------------------AYVIYTSGSTGKPKG 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 227 VLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIvnKLLCPLWV-GATCVMLPDFQAQKVWEMLLSSKAPTINVF 305
Cdd:cd17644 124 VMIEHQSLVNLSHGLIKEYGITSSDRVLQFASIAFDVAA--EEIYVTLLsGATLVLRPEEMRSSLEDFVQYIQQWQLTVL 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 306 MAVPTiyskliqfydqhYQQPLLKEFVKAVCK--ERIRLMVSGSAALPLPTLQRWKEITGH--TLLERYGMTEIGMA--- 378
Cdd:cd17644 202 SLPPA------------YWHLLVLELLLSTIDlpSSLRLVIVGGEAVQPELVRQWQKNVGNfiQLINVYGPTEATIAatv 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 379 --LSNPLTGPRIPGSVGVPLPGVDVRIVMNNKINTSIvegnh*ntwvrpgleGKEGELLVRGQSVFKEYWNKPQETRESF 456
Cdd:cd17644 270 crLTQLTERNITSVPIGRPIANTQVYILDENLQPVPV---------------GVPGELHIGGVGLARGYLNRPELTAEKF 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 457 TddgwfktgTEPSLSSPafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlprEERcqtsfspmlqtgltptmt 536
Cdd:cd17644 335 I--------SHPFNSSE------------------------------------------SER------------------ 346
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 537 *ltslqrfahIHPLGDTAIY-KDGVYWIMGRTSVDIlKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVV 615
Cdd:cd17644 347 ----------LYKTGDLARYlPDGNIEYLGRIDNQV-KIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQPGNKRLVAYI 415
|
570 580 590 600
....*....|....*....|....*....|....*....|....*....
gi 928020699 616 QLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd17644 416 VPHYEESPSTVELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
2-664 |
6.54e-19 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 91.94 E-value: 6.54e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 2 LPELFAFSKQTLM--WTSRWKATPYRTlfySRQRLLEATVRRNSHRSALApswvekqkpvftkapeFGEKlavidscgKH 79
Cdd:PRK12316 485 LPMLDAEERGQLVegWNATAAEYPLQR---GVHRLFEEQVERTPEAPALA----------------FGEE--------TL 537
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYCSSLGLASR-ISSALGSDfggldgkRISFLCANDS-SYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSS 157
Cdd:PRK12316 538 DYAELNRRANRLAHAlIERGVGPD-------VLVGVAMERSiEMVVALLAILKAGGAYVPLDPEYPAERLAYMLEDSGVQ 610
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 158 LVVAGHPYEEILqPLAQKlglpclplppTNNLDaLDETD-------TREKEITITdwTDRPAMIIYTSGTTGRPKGVLHT 230
Cdd:PRK12316 611 LLLSQSHLGRKL-PLAAG----------VQVLD-LDRPAawlegysEENPGTELN--PENLAYVIYTSGSTGKPKGAGNR 676
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 231 HSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGiVNKLLCPLWVGATCVMLPD---FQAQKVWEMllsSKAPTINVFMA 307
Cdd:PRK12316 677 HRALSNRLCWMQQAYGLGVGDTVLQKTPFSFDVS-VWEFFWPLMSGARLVVAAPgdhRDPAKLVEL---INREGVDTLHF 752
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 308 VPTIYSKLIQFYDQHYQQPLlkefvkavckeriRLMVSGSAALPLPTLQR--WKEITGHtLLERYGMTEIGMALSN---- 381
Cdd:PRK12316 753 VPSMLQAFLQDEDVASCTSL-------------RRIVCSGEALPADAQEQvfAKLPQAG-LYNLYGPTEAAIDVTHwtcv 818
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 382 PLTGPRIPgsVGVPLPGVdvrivmnnkiNTSIVEGNh*ntwVRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFtddgw 461
Cdd:PRK12316 819 EEGGDSVP--IGRPIANL----------ACYILDAN-----LEPVPVGVLGELYLAGRGLARGYHGRPGLTAERF----- 876
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 462 fktgtepsLSSPafvlthFSAAdvrtlidmlpgkgvnsaycsctirrcrgtlpreERcqtsfspMLQTgltptmt*ltsl 541
Cdd:PRK12316 877 --------VPSP------FVAG---------------------------------ER-------MYRT------------ 890
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 542 qrfahihplGDTAIYK-DGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIgapdATWGQKVTAVVQLRKG 620
Cdd:PRK12316 891 ---------GDLARYRaDGVIEYAGRID-HQVKLRGLRIELGEIEARLLEHPWVREAAVL----AVDGKQLVGYVVLESE 956
|
650 660 670 680
....*....|....*....|....*....|....*....|....
gi 928020699 621 KSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK12316 957 GGDWREALKAHLAASLPEYMVPAQWLALERLPLTPNGKLDRKAL 1000
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
212-668 |
6.54e-19 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 90.58 E-value: 6.54e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 212 AMIIYTSGTTGRPKGVLHTHSS--IQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLwVGATCVML-PDFQAQ 288
Cdd:PRK06018 180 AGMCYTSGTTGDPKGVLYSHRSnvLHALMANNGDALGTSAADTMLPVVPLFHANSWGIAFSAPS-MGTKLVMPgAKLDGA 258
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 289 KVWEMLLSSKaptINVFMAVPTIYSKLIQFYDQHYQQ-PLLKefvKAVCkerirlmvsGSAALPLPTLQRWKEItGHTLL 367
Cdd:PRK06018 259 SVYELLDTEK---VTFTAGVPTVWLMLLQYMEKEGLKlPHLK---MVVC---------GGSAMPRSMIKAFEDM-GVEVR 322
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 368 ERYGMTE---IGM--ALSNPLTGprIPGSV--------GVPLPGVDVRIVMNnkintsivEGNH*nTWvrpglEGKE-GE 433
Cdd:PRK06018 323 HAWGMTEmspLGTlaALKPPFSK--LPGDArldvlqkqGYPPFGVEMKITDD--------AGKEL-PW-----DGKTfGR 386
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 434 LLVRGQSVFKEYWnkpQETRESFTDDGWFKTGtepslsspafvlthfsaaDVRTlIDmlpgkgvnsaycsctirrcrgtl 513
Cdd:PRK06018 387 LKVRGPAVAAAYY---RVDGEILDDDGFFDTG------------------DVAT-ID----------------------- 421
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 514 preercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiyKDGVYWIMGRtSVDILKSGGFKISALEVECHLLAHPD 593
Cdd:PRK06018 422 -------------------------------------------AYGYMRITDR-SKDVIKSGGEWISSIDLENLAVGHPK 457
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 928020699 594 IADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLKHF 668
Cdd:PRK06018 458 VAEAAVIGVYHPKWDERPLLIVQLKPGETATREEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALREQF 532
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
209-465 |
7.81e-19 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 91.52 E-value: 7.81e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTH----SSIQAMTQcLVSAwawSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPD 284
Cdd:PRK08633 782 DDTATIIFSSGSEGEPKGVMLSHhnilSNIEQISD-VFNL---RNDDVILSSLPFFHSFGLTVTLWLPLLEGIKVVYHPD 857
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 285 -FQAQKVWEMLLSSKApTInvFMAVPT---IYSKLIQfydqhyqqpllkefVKAVCKERIRLMVSGSAALPLPTLQRWKE 360
Cdd:PRK08633 858 pTDALGIAKLVAKHRA-TI--LLGTPTflrLYLRNKK--------------LHPLMFASLRLVVAGAEKLKPEVADAFEE 920
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 361 ITGHTLLERYGMTE-----------IGMALSNPLTGPRiPGSVGVPLPGVDVRIVmnnkintsivegnH*NTWVrPGLEG 429
Cdd:PRK08633 921 KFGIRILEGYGATEtspvasvnlpdVLAADFKRQTGSK-EGSVGMPLPGVAVRIV-------------DPETFE-ELPPG 985
|
250 260 270
....*....|....*....|....*....|....*....
gi 928020699 430 KEGELLVRGQSVFKEYWNKPQETRESFTD---DGWFKTG 465
Cdd:PRK08633 986 EDGLILIGGPQVMKGYLGDPEKTAEVIKDidgIGWYVTG 1024
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
68-664 |
1.13e-18 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 89.06 E-value: 1.13e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLAsRISSALGSDFGGLDGkrisfLCANDSSY-VVAQWAVWMSGGTAVPLYRKHPPSE 146
Cdd:cd17650 2 DAIAVSDATRQLTYRELNERANQLA-RTLRGLGVAPGSVVG-----VCADRSLDaIVGLLAVLKAGGAYVPIDPDYPAER 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 147 LEYIISDSQSSLVVaghpyeeiLQPlaqklglpclplpptnnldaldetdtrekeititdwtDRPAMIIYTSGTTGRPKG 226
Cdd:cd17650 76 LQYMLEDSGAKLLL--------TQP-------------------------------------EDLAYVIYTSGTTGKPKG 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 227 VLHTHSSIQAMtqclvsAWAWSKNDIILHTLPLHHLHG------IVNKLLCPLWVGATCVMLPD---FQAQKVWEMLLSS 297
Cdd:cd17650 111 VMVEHRNVAHA------AHAWRREYELDSFPVRLLQMAsfsfdvFAGDFARSLLNGGTLVICPDevkLDPAALYDLILKS 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 298 KaptINVFMAVPTIYSKLIQFYDQHYQQPllkefvkavckERIRLMVSGSAALPLptlqRWK-----EITGHT-LLERYG 371
Cdd:cd17650 185 R---ITLMESTPALIRPVMAYVYRNGLDL-----------SAMRLLIVGSDGCKA----QDFktlaaRFGQGMrIINSYG 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 372 MTEIGMALSN-PLTGPRIPGS----VGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGKEGELLVRGQSVFKEYW 446
Cdd:cd17650 247 VTEATIDSTYyEEGRDPLGDSanvpIGRPLPNTAMYVL---------------DERLQPQPVGVAGELYIGGAGVARGYL 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 447 NKPQETRESFTDDgwfktgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtSFSPM 526
Cdd:cd17650 312 NRPELTAERFVEN--------------------------------------------------------------PFAPG 329
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 527 lqtgltptmt*ltslqrfAHIHPLGDTAIYK-DGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDA 605
Cdd:cd17650 330 ------------------ERMYRTGDLARWRaDGNVELLGRVD-HQVKIRGFRIELGEIESQLARHPAIDEAVVAVREDK 390
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*....
gi 928020699 606 twGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd17650 391 --GGEARLCAYVVAAATLNTAELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
92-661 |
1.52e-18 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 89.43 E-value: 1.52e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 92 ASRISSALgSDFGGLDGKRISFLCANDSSYV-VAQWAVWMsGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQ 170
Cdd:PRK06155 56 AAAAAHAL-AAAGVKRGDRVALMCGNRIEFLdVFLGCAWL-GAIAVPINTALRGPQLEHILRNSGARLLVVEAALLAALE 133
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 171 P-------------LAQKLGLPCLPLPPTNNLDALDETDTrEKEITITDwtdrPAMIIYTSGTTGRPKGVLHTHSSIQAM 237
Cdd:PRK06155 134 AadpgdlplpavwlLDAPASVSVPAGWSTAPLPPLDAPAP-AAAVQPGD----TAAILYTSGTTGPSKGVCCPHAQFYWW 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 238 TQCLVSAWAWSKNDIILHTLPLHHLHGIvNKLLCPLWVGATCVMLPDFQAQKVWEMLLSSKAPTINVFMAVPTIyskliq 317
Cdd:PRK06155 209 GRNSAEDLEIGADDVLYTTLPLFHTNAL-NAFFQALLAGATYVLEPRFSASGFWPAVRRHGATVTYLLGAMVSI------ 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 318 fydqhyqqpLLKEFVKAVCKERiRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGMALSNPLTGPRiPGSVGVPLP 397
Cdd:PRK06155 282 ---------LLSQPARESDRAH-RVRVALGPGVPAALHAAFRERFGVDLLDGYGSTETNFVIAVTHGSQR-PGSMGRLAP 350
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 398 GVDVRIVMNNKINTSivegnh*ntwvrpglEGKEGELLVRGQSVF---KEYWNKPQETRESFtDDGWFKTGTepslsspa 474
Cdd:PRK06155 351 GFEARVVDEHDQELP---------------DGEPGELLLRADEPFafaTGYFGMPEKTVEAW-RNLWFHTGD-------- 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 475 fvlthfsaadvRTLIDmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdta 554
Cdd:PRK06155 407 -----------RVVRD---------------------------------------------------------------- 411
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 555 iyKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWARE 634
Cdd:PRK06155 412 --ADGWFRFVDRIK-DAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEDEVMAAVVLRDGTALEPVALVRHCEP 488
|
570 580
....*....|....*....|....*..
gi 928020699 635 HMGPYKIPTGLLLVEEMPRNQMGKINK 661
Cdd:PRK06155 489 RLAYFAVPRYVEFVAALPKTENGKVQK 515
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
19-664 |
1.59e-18 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 90.79 E-value: 1.59e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 19 WKATPYRtlfYSRQR----LLEATVRRNSHRSALApswvekqkpvftkapeFGEKlavidscgKHSYKQLYCSSLGLASR 94
Cdd:PRK12316 4540 WNRTDAG---YPATRcvhqLVAERARMTPDAVAVV----------------FDEE--------KLTYAELNRRANRLAHA 4592
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 95 -ISSALGSDFggldgkRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVV------AGHPYEE 167
Cdd:PRK12316 4593 lIARGVGPEV------LVGIAMERSAEMMVGLLAVLKAGGAYVPLDPEYPRERLAYMMEDSGAALLLtqshllQRLPIPD 4666
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 168 ILQPLAqklglpclplpptnnldaLDETDtrekeititDWTDRP-------------AMIIYTSGTTGRPKGVLHTHSSI 234
Cdd:PRK12316 4667 GLASLA------------------LDRDE---------DWEGFPahdpavrlhpdnlAYVIYTSGSTGRPKGVAVSHGSL 4719
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 235 QAMTQCLVSAWAWSKNDIIlHTLPLHHLHGIVNKLLCPLWVGAtCVMLPDFQAQKVWEMLLSSKAPTINVFMAVPTiysk 314
Cdd:PRK12316 4720 VNHLHATGERYELTPDDRV-LQFMSFSFDGSHEGLYHPLINGA-SVVIRDDSLWDPERLYAEIHEHRVTVLVFPPV---- 4793
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 315 liqfydqhYQQPLLKEFVKAVCKERIRLMVSGSAALPLPTLQR-WKEITGHTLLERYGMTEigmALSNPLT----GPRIP 389
Cdd:PRK12316 4794 --------YLQQLAEHAERDGEPPSLRVYCFGGEAVAQASYDLaWRALKPVYLFNGYGPTE---TTVTVLLwkarDGDAC 4862
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 390 GS----VGVPLPGVDVRiVMNNKINtsivegnh*ntwvrPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTG 465
Cdd:PRK12316 4863 GAaympIGTPLGNRSGY-VLDGQLN--------------PLPVGVAGELYLGGEGVARGYLERPALTAERFVPDPFGAPG 4927
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 466 tepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrGTLPREercqtsfspmlqtgltptmt*ltslqrfa 545
Cdd:PRK12316 4928 ---------------------------------------------GRLYRT----------------------------- 4933
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 546 hihplGDTAIYK-DGVYWIMGRtsVD-ILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKV-------TAVVQ 616
Cdd:PRK12316 4934 -----GDLARYRaDGVIDYLGR--VDhQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAVGKQLVgyvvpqdPALAD 5006
|
650 660 670 680
....*....|....*....|....*....|....*....|....*...
gi 928020699 617 LRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK12316 5007 ADEAQAELRDELKAALRERLPEYMVPAHLVFLARMPLTPNGKLDRKAL 5054
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
211-666 |
1.65e-18 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 89.68 E-value: 1.65e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 211 PAMIIYTSGTTGRPKGVL-----------HTHSSIQAMTQCLVsaWaWSKNDIilhtlplhhlhGIV--NKLLC--PLWV 275
Cdd:cd05967 232 PLYILYTSGTTGKPKGVVrdngghavalnWSMRNIYGIKPGDV--W-WAASDV-----------GWVvgHSYIVygPLLH 297
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 276 GATCVML-------PDfqAQKVWEMLlsSKAPTINVFMAvPTIYSKLiqfydqhYQQPLLKEFVKAVCKERIRLMVSGSA 348
Cdd:cd05967 298 GATTVLYegkpvgtPD--PGAFWRVI--EKYQVNALFTA-PTAIRAI-------RKEDPDGKYIKKYDLSSLRTLFLAGE 365
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 349 ALPLPTLQRWKEITGHTLLERYGMTEIGMALSNPLTG----PRIPGSVGVPLPGVDVRIVmnnKINTSIVEGNh*ntwvr 424
Cdd:cd05967 366 RLDPPTLEWAENTLGVPVIDHWWQTETGWPITANPVGleplPIKAGSPGKPVPGYQVQVL---DEDGEPVGPN------- 435
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 425 pglegKEGELLVRGqsvfkeywnkpqetresftddgwfktgtepslssPafvlthfsaadvrtlidMLPGkgvnsaycsc 504
Cdd:cd05967 436 -----ELGNIVIKL----------------------------------P-----------------LPPG---------- 449
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 505 tirrCRGTLPR-EERCQTSFspmlqtgltptmt*ltsLQRFAHIHPLGDTAIY-KDGVYWIMGRTSvDILKSGGFKISAL 582
Cdd:cd05967 450 ----CLLTLWKnDERFKKLY-----------------LSKFPGYYDTGDAGYKdEDGYLFIMGRTD-DVINVAGHRLSTG 507
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 583 EVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQT----WAREHMGPYKIPTGLLLVEEMPRNQMGK 658
Cdd:cd05967 508 EMEESVLSHPAVAECAVVGVRDELKGQVPLGLVVLKEGVKITAEELEKelvaLVREQIGPVAAFRLVIFVKRLPKTRSGK 587
|
....*...
gi 928020699 659 INKKDLLK 666
Cdd:cd05967 588 ILRRTLRK 595
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
557-666 |
1.94e-18 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 88.51 E-value: 1.94e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 557 KDGVYWIMGRTSVDILkSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGkSLTLPELQTWAREHM 636
Cdd:PRK07445 336 AQGYLHILGRNSQKII-TGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDP-SISLEELKTAIKDQL 413
|
90 100 110
....*....|....*....|....*....|
gi 928020699 637 GPYKIPTGLLLVEEMPRNQMGKINKKDLLK 666
Cdd:PRK07445 414 SPFKQPKHWIPVPQLPRNPQGKINRQQLQQ 443
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
108-664 |
2.52e-18 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 88.65 E-value: 2.52e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 108 GKRISFLCANDSSYVVAQWAVWMSGGTAVPL---YRKHppsELEYIISDSQSSLVVA-----GHPYEEILQPLAQKLGLP 179
Cdd:PRK06164 60 GDRVAVWLPNCIEWVVLFLACARLGATVIAVntrYRSH---EVAHILGRGRARWLVVwpgfkGIDFAAILAAVPPDALPP 136
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 180 CL---------------PLPPTNNLDALDETDTREKEITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSA 244
Cdd:PRK06164 137 LRaiavvddaadatpapAPGARVQLFALPDPAPPAAAGERAADPDAGALLFTTSGTTSGPKLVLHRQATLLRHARAIARA 216
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 245 WAWSKNDIILHTLPLHHLHGIvNKLLCPLWVGATCVMLPDFQAQKVWEMLLSSKaptINVFMAVPTIYSKLIQFYDQHYQ 324
Cdd:PRK06164 217 YGYDPGAVLLAALPFCGVFGF-STLLGALAGGAPLVCEPVFDAARTARALRRHR---VTHTFGNDEMLRRILDTAGERAD 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 325 QPLLKEFVKAVckerirlMVSGSAALPLPTLQRWKEITGhtlleRYGMTE-IGMALSNPLTGP---RIPGSvGVPL-PGV 399
Cdd:PRK06164 293 FPSARLFGFAS-------FAPALGELAALARARGVPLTG-----LYGSSEvQALVALQPATDPvsvRIEGG-GRPAsPEA 359
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 400 DVRIVmnNKINTSIVEgnh*ntwvrpglEGKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGTepslsspafvlth 479
Cdd:PRK06164 360 RVRAR--DPQDGALLP------------DGESGEIEIRAPSLMRGYLDNPDATARALTDDGYFRTGD------------- 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 480 fsaadvrtlidmlpgkgvnsaycsctIRRCRGtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDG 559
Cdd:PRK06164 413 --------------------------LGYTRG----------------------------------------------DG 420
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 560 VYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGApdaTWGQKVTAV--VQLRKGKSLTLPELQTWAREHMG 637
Cdd:PRK06164 421 QFVYQTRMG-DSLRLGGFLVNPAEIEHALEALPGVAAAQVVGA---TRDGKTVPVafVIPTDGASPDEAGLMAACREALA 496
|
570 580 590
....*....|....*....|....*....|
gi 928020699 638 PYKIPTGLLLVEEMPRNQMG---KINKKDL 664
Cdd:PRK06164 497 GFKVPARVQVVEAFPVTESAngaKIQKHRL 526
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
90-465 |
3.40e-18 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 88.63 E-value: 3.40e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 90 GLASRISS-ALG-SDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAG----- 162
Cdd:cd17641 16 DYADRVRAfALGlLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIAEdeeqv 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 163 ----------HPYEEIL--QPLAQKLGLPCLPLPPTNNLDALDETDTR-----EKEITITDWTDrPAMIIYTSGTTGRPK 225
Cdd:cd17641 96 dklleiadriPSVRYVIycDPRGMRKYDDPRLISFEDVVALGRALDRRdpglyEREVAAGKGED-VAVLCTTSGTTGKPK 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 226 GVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGAT--CV-----MLPD---------FQAQK 289
Cdd:cd17641 175 LAMLSHGNFLGHCAAYLAADPLGPGDEYVSVLPLPWIGEQMYSVGQALVCGFIvnFPeepetMMEDlreigptfvLLPPR 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 290 VWEMLLS------SKAPTINVFM----------AVPTIYSKLIQFYDQHYQQPLLKEFVKAVCKERI-----RLMVSGSA 348
Cdd:cd17641 255 VWEGIAAdvrarmMDATPFKRFMfelgmklglrALDRGKRGRPVSLWLRLASWLADALLFRPLRDRLgfsrlRSAATGGA 334
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 349 ALPlPTLQRWKEITGHTLLERYGMTEIGMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnkintsivegnh*ntwvrpgle 428
Cdd:cd17641 335 ALG-PDTFRFFHAIGVPLKQLYGQTELAGAYTVHRDGDVDPDTVGVPFPGTEVRID------------------------ 389
|
410 420 430
....*....|....*....|....*....|....*..
gi 928020699 429 gKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTG 465
Cdd:cd17641 390 -EVGEILVRSPGVFVGYYKNPEATAEDFDEDGWLHTG 425
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
122-664 |
4.57e-18 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 89.45 E-value: 4.57e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILqPLAQKLGLPClplpptnnLDALD---ETDTR 198
Cdd:PRK12467 1638 VVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQSHLQARL-PLPDGLRSLV--------LDQEDdwlEGYSD 1708
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 199 EKEITITDwTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIIlHTLPLHHLHGIVNKLLCPLWVGAT 278
Cdd:PRK12467 1709 SNPAVNLA-PQNLAYVIYTSGSTGRPKGAGNRHGALVNRLCATQEAYQLSAADVV-LQFTSFAFDVSVWELFWPLINGAR 1786
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 279 CVMLPDFQAQKVWEMLLSSKAPTINVFMAVPTIYSKLIQfYDQHYQQPLlkefvkavckeRIRLMVSGSAALPLPTLQRW 358
Cdd:PRK12467 1787 LVIAPPGAHRDPEQLIQLIERQQVTTLHFVPSMLQQLLQ-MDEQVEHPL-----------SLRRVVCGGEALEVEALRPW 1854
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 359 KEITGHT-LLERYGMTEIGMALS------NPLTGpRIPGSVGVPLPGVDVRIvMNNKINtsivegnh*ntwvrPGLEGKE 431
Cdd:PRK12467 1855 LERLPDTgLFNLYGPTETAVDVThwtcrrKDLEG-RDSVPIGQPIANLSTYI-LDASLN--------------PVPIGVA 1918
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 432 GELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrG 511
Cdd:PRK12467 1919 GELYLGGVGLARGYLNRPALTAERFVADPFGTVG---------------------------------------------S 1953
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 512 TLPREercqtsfspmlqtgltptmt*ltslqrfahihplGDTAIYK-DGVYWIMGRTSVDIlKSGGFKISALEVECHLLA 590
Cdd:PRK12467 1954 RLYRT----------------------------------GDLARYRaDGVIEYLGRIDHQV-KIRGFRIELGEIEARLRE 1998
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 591 HPDIADVAVIgAPDATWGQKVTAVV-------------------QLRKGKSLTLPElqtwarehmgpYKIPTGLLLVEEM 651
Cdd:PRK12467 1999 QGGVREAVVI-AQDGANGKQLVAYVvptdpglvdddeaqvalraILKNHLKASLPE-----------YMVPAHLVFLARM 2066
|
570
....*....|...
gi 928020699 652 PRNQMGKINKKDL 664
Cdd:PRK12467 2067 PLTPNGKLDRKAL 2079
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
216-668 |
5.50e-18 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 87.84 E-value: 5.50e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 216 YTSGTTGRPKGVLHTHSS--IQAMTQCLVSAWAWSKNDIILHTLPLHHlhgiVNK----LLCPLwVGATCVML-PDFQAQ 288
Cdd:PRK07008 183 YTSGTTGNPKGALYSHRStvLHAYGAALPDAMGLSARDAVLPVVPMFH----VNAwglpYSAPL-TGAKLVLPgPDLDGK 257
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 289 KVWEMLLSSKAptinVFMA-VPTIYSKLIQfydqHYQQPLLKeFVKavckerIRLMVSGSAALPLPTLQRWKEITGHTLL 367
Cdd:PRK07008 258 SLYELIEAERV----TFSAgVPTVWLGLLN----HMREAGLR-FST------LRRTVIGGSACPPAMIRTFEDEYGVEVI 322
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 368 ERYGMTEI----------GMALSNPLTGPR-IPGSVGVPLPGVDVRIVmnnkintsivegnh*ntwvrpGLEGKE----- 431
Cdd:PRK07008 323 HAWGMTEMsplgtlcklkWKHSQLPLDEQRkLLEKQGRVIYGVDMKIV---------------------GDDGRElpwdg 381
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 432 ---GELLVRGQSVFKEYWNKpqetRESFTDDGWFKTGtepslsspafvlthfsaaDVRTlIDmlpgkgvnsaycsctirr 508
Cdd:PRK07008 382 kafGDLQVRGPWVIDRYFRG----DASPLVDGWFPTG------------------DVAT-ID------------------ 420
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 509 crgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiyKDGVYWIMGRtSVDILKSGGFKISALEVECHL 588
Cdd:PRK07008 421 ------------------------------------------------ADGFMQITDR-SKDVIKSGGEWISSIDIENVA 451
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 589 LAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLKHF 668
Cdd:PRK07008 452 VAHPAVAEAACIACAHPKWDERPLLVVVKRPGAEVTREELLAFYEGKVAKWWIPDDVVFVDAIPHTATGKLQKLKLREQF 531
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
551-664 |
8.86e-18 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 87.12 E-value: 8.86e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 551 GDTAIYKD-GVYWIMGRtSVDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQ 629
Cdd:PRK13382 421 GDVGYLDEnGRLFVVGR-DDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKPGASATPETLK 499
|
90 100 110
....*....|....*....|....*....|....*
gi 928020699 630 TWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK13382 500 QHVRDNLANYKVPRDIVVLDELPRGATGKILRREL 534
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
552-668 |
1.27e-17 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 85.10 E-value: 1.27e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 552 DTAIYKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTW 631
Cdd:PRK07824 240 DLGALDDGVLTVLGRAD-DAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAVVGDGGPAPTLEALRAH 318
|
90 100 110
....*....|....*....|....*....|....*..
gi 928020699 632 AREHMGPYKIPTGLLLVEEMPRNQMGKINKKDLLKHF 668
Cdd:PRK07824 319 VARTLDRTAAPRELHVVDELPRRGIGKVDRRALVRRF 355
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
108-664 |
2.63e-17 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 85.51 E-value: 2.63e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 108 GKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQPLAQKLGLPCLPLPptn 187
Cdd:PRK13391 49 GDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARALITSAAKLDVARALLKQCPGVRHRLV--- 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 188 nLDALDETDTREK-EITITDWTDRP-------AMIIYTSGTTGRPKGVLHTHSSIQAMTQC-----LVSAWAWsKNDIIL 254
Cdd:PRK13391 126 -LDGDGELEGFVGyAEAVAGLPATPiadeslgTDMLYSSGTTGRPKGIKRPLPEQPPDTPLpltafLQRLWGF-RSDMVY 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 255 HT------LPLHHLHGIVNKLlcplwvGATCVMLPDFQAQKVWEMLLSSKaptINVFMAVPTIYSKLIQfydqhyqqpLL 328
Cdd:PRK13391 204 LSpaplyhSAPQRAVMLVIRL------GGTVIVMEHFDAEQYLALIEEYG---VTHTQLVPTMFSRMLK---------LP 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 329 KEFVKAVCKERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTE-IGMALSNPLTGPRIPGSVGVPLPGvDVRIVMNN 407
Cdd:PRK13391 266 EEVRDKYDLSSLEVAIHAAAPCPPQVKEQMIDWWGPIIHEYYAATEgLGFTACDSEEWLAHPGTVGRAMFG-DLHILDDD 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 408 kintsivegnh*ntwVRPGLEGKEGELLVRGQSVFkEYWNKPQETRESFTDDgwfktgtepslsspafvlthfsaadvrt 487
Cdd:PRK13391 345 ---------------GAELPPGEPGTIWFEGGRPF-EYLNDPAKTAEARHPD---------------------------- 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 488 lidmlpgkgvnsaycsctirrcrgtlpreercqtsfspmlqtgltPTMT*ltslqrfahihpLGDTA-IYKDGVYWIMGR 566
Cdd:PRK13391 381 ---------------------------------------------GTWST------------VGDIGyVDEDGYLYLTDR 403
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 567 TSVDILkSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLT---LPELQTWAREHMGPYKIPT 643
Cdd:PRK13391 404 AAFMII-SGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDGVDPGpalAAELIAFCRQRLSRQKCPR 482
|
570 580
....*....|....*....|.
gi 928020699 644 GLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK13391 483 SIDFEDELPRLPTGKLYKRLL 503
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
209-667 |
6.59e-17 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 83.77 E-value: 6.59e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTqclVSAWAW---SKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVML--P 283
Cdd:cd05974 85 DDPMLLYFTSGTTSKPKLVEHTHRSYPVGH---LSTMYWiglKPGDVHWNISSPGWAKHAWSCFFAPWNAGATVFLFnyA 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 284 DFQAQKVWEMLLSSKAPTinvFMAVPTIYSKLIQfydqhyqQPLlkefvkAVCKERIRLMVSGSAALPLPTLQRWKEITG 363
Cdd:cd05974 162 RFDAKRVLAALVRYGVTT---LCAPPTVWRMLIQ-------QDL------ASFDVKLREVVGAGEPLNPEVIEQVRRAWG 225
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 364 HTLLERYGMTEIGMALSNPLTGPRIPGSVGVPLPGVdvRIVMNNKIntsivegnh*ntwvrpGLEGKEGELLV-----RG 438
Cdd:cd05974 226 LTIRDGYGQTETTALVGNSPGQPVKAGSMGRPLPGY--RVALLDPD----------------GAPATEGEVALdlgdtRP 287
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 439 QSVFKEYWNKPQETRESFtDDGWFKTGtepslsspafvlthfsaaDVRTlidmlpgkgvnsaycsctirrcrgtlpREEr 518
Cdd:cd05974 288 VGLMKGYAGDPDKTAHAM-RGGYYRTG------------------DIAM---------------------------RDE- 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 519 cqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVA 598
Cdd:cd05974 321 ---------------------------------------DGYLTYVGRAD-DVFKSSDYRISPFELESVLIEHPAVAEAA 360
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 928020699 599 VIGAPDATWGQKVTAVVQLRKG---KSLTLPELQTWAREHMGPYKIPTGLLLVeEMPRNQMGKINKKDLLKH 667
Cdd:cd05974 361 VVPSPDPVRLSVPKAFIVLRAGyepSPETALEIFRFSRERLAPYKRIRRLEFA-ELPKTISGKIRRVELRRR 431
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
80-465 |
7.12e-17 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 84.51 E-value: 7.12e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYCSSLglasRISSALGSDfgGLD-GKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSL 158
Cdd:PLN02861 79 TYKEVYDAAI----RIGSAIRSR--GVNpGDRCGIYGSNCPEWIIAMEACNSQGITYVPLYDTLGANAVEFIINHAEVSI 152
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 159 V-VAGHPYEEILQPLaQKLGLPCLPLPPTNNLDALDETDTREKEITITDWTDRPAM-----------------IIYTSGT 220
Cdd:PLN02861 153 AfVQESKISSILSCL-PKCSSNLKTIVSFGDVSSEQKEEAEELGVSCFSWEEFSLMgsldcelppkqktdictIMYTSGT 231
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 221 TGRPKGVLHTHSSIQA--MTQ---CLVSAWAWSKNDIILHTLPLHHLHGIVNKLLC-------PLWVGATCVMLPDFQAQ 288
Cdd:PLN02861 232 TGEPKGVILTNRAIIAevLSTdhlLKVTDRVATEEDSYFSYLPLAHVYDQVIETYCiskgasiGFWQGDIRYLMEDVQAL 311
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 289 KvwemllsskaPTInvFMAVPTIY------------------SKLIQF------------YDQHYQQPLLKEFVKAVCKE 338
Cdd:PLN02861 312 K----------PTI--FCGVPRVYdriytgimqkissggmlrKKLFDFaynyklgnlrkgLKQEEASPRLDRLVFDKIKE 379
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 339 ----RIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTE-IGMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnkintSI 413
Cdd:PLN02861 380 glggRVRLLLSGAAPLPRHVEEFLRVTSCSVLSQGYGLTEsCGGCFTSIANVFSMVGTVGVPMTTIEARLE-------SV 452
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|..
gi 928020699 414 VEGNH*NTWVRPglegkEGELLVRGQSVFKEYWNKPQETRESFTdDGWFKTG 465
Cdd:PLN02861 453 PEMGYDALSDVP-----RGEICLRGNTLFSGYHKRQDLTEEVLI-DGWFHTG 498
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
212-465 |
7.21e-17 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 84.38 E-value: 7.21e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 212 AMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCpLWVGATCvmlpDFQAQKVW 291
Cdd:PLN02736 224 ATICYTSGTTGTPKGVVLTHGNLIANVAGSSLSTKFYPSDVHISYLPLAHIYERVNQIVM-LHYGVAV----GFYQGDNL 298
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 292 EML--LSSKAPTInvFMAVPTIYSKLiqfYDQ---------------------HYQQPLLK-------------EFVKAV 335
Cdd:PLN02736 299 KLMddLAALRPTI--FCSVPRLYNRI---YDGitnavkesgglkerlfnaaynAKKQALENgknpspmwdrlvfNKIKAK 373
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 336 CKERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEIGMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnkintSIVE 415
Cdd:PLN02736 374 LGGRVRFMSSGASPLSPDVMEFLRICFGGRVLEGYGMTETSCVISGMDEGDNLSGHVGSPNPACEVKLV-------DVPE 446
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|...
gi 928020699 416 GNH*NtwvrpglEGK---EGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTG 465
Cdd:PLN02736 447 MNYTS-------EDQpypRGEICVRGPIIFKGYYKDEVQTREVIDEDGWLHTG 492
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
209-464 |
7.60e-17 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 84.70 E-value: 7.60e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLV-SAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQA 287
Cdd:PRK06060 145 DALAYATYTSGTTGPPKAAIHRHADPLTFVDAMCrKALRLTPEDTGLCSARMYFAYGLGNSVWFPLATGGSAVINSAPVT 224
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 288 QKVWEMLLSSKAPTinVFMAVPTIYSKLIqfydqhyqqpllkEFVKAVCKERIRLMVSGSAALPLPTLQRWKEITGHT-L 366
Cdd:PRK06060 225 PEAAAILSARFGPS--VLYGVPNFFARVI-------------DSCSPDSFRSLRCVVSAGEALELGLAERLMEFFGGIpI 289
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 367 LERYGMTEIGMA-LSNPLTGPRiPGSVGVPLPGVDVRIVMNNKINTSivegnh*ntwvrpglEGKEGELLVRGQSVFKEY 445
Cdd:PRK06060 290 LDGIGSTEVGQTfVSNRVDEWR-LGTLGRVLPPYEIRVVAPDGTTAG---------------PGVEGDLWVRGPAIAKGY 353
|
250
....*....|....*....
gi 928020699 446 WNKPQETresFTDDGWFKT 464
Cdd:PRK06060 354 WNRPDSP---VANEGWLDT 369
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
68-669 |
9.59e-17 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 83.36 E-value: 9.59e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfgGLDGKRISFLCANDSSY-VVAQWAVWMSGGTAVPLYRKHPPSE 146
Cdd:cd05918 14 DAPAVCAWDGSLTYAELDRLSSRLAHHLRSL------GVGPGVFVPLCFEKSKWaVVAMLAVLKAGGAFVPLDPSHPLQR 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 147 LEYIISDSQSSLVVAGHPyeeilqplaqklglpclplpptnnldaldetdtrekeititdwtDRPAMIIYTSGTTGRPKG 226
Cdd:cd05918 88 LQEILQDTGAKVVLTSSP--------------------------------------------SDAAYVIFTSGSTGKPKG 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 227 VLHTHSSIQAMTQCLVSAWAWSKN-----------DIilhtlplhhlhgIVNKLLCPLWVGAT-CVM--------LPDF- 285
Cdd:cd05918 124 VVIEHRALSTSALAHGRALGLTSEsrvlqfasytfDV------------SILEIFTTLAAGGClCIPseedrlndLAGFi 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 286 QAQKV-WEMLLSSKAPTINVfMAVPTiyskliqfydqhyqqpllkefvkavckerIRLMVSGSAALPLPTLQRWKEitGH 364
Cdd:cd05918 192 NRLRVtWAFLTPSVARLLDP-EDVPS-----------------------------LRTLVLGGEALTQSDVDTWAD--RV 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 365 TLLERYGMTE--IGMALSNPLTGPRiPGSVGVPLPGVdvrivmnnkinTSIVE-GNH*ntwvRPGLEGKEGELLVRGQSV 441
Cdd:cd05918 240 RLINAYGPAEctIAATVSPVVPSTD-PRNIGRPLGAT-----------CWVVDpDNHD----RLVPIGAVGELLIEGPIL 303
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 442 FKEYWNKPQETRESFTDDgwfktgtepslssPAFVLthfsaadvrtlidmlpgkgvnsaycsctirrcRGTLPREERcqt 521
Cdd:cd05918 304 ARGYLNDPEKTAAAFIED-------------PAWLK--------------------------------QEGSGRGRR--- 335
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 522 sfspMLQTGltptmt*ltSLQRFAHihplgdtaiykDGVYWIMGR--TSVdilKSGGFKISALEVECHLLAHPDIAD--- 596
Cdd:cd05918 336 ----LYRTG---------DLVRYNP-----------DGSLEYVGRkdTQV---KIRGQRVELGEIEHHLRQSLPGAKevv 388
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 597 VAVIGAPDATWGQKVTAVVQLRKGKS-----------------LTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKI 659
Cdd:cd05918 389 VEVVKPKDGSSSPQLVAFVVLDGSSSgsgdgdslflepsdefrALVAELRSKLRQRLPSYMVPSVFLPLSHLPLTASGKI 468
|
650
....*....|
gi 928020699 660 NKKdLLKHFF 669
Cdd:cd05918 469 DRR-ALRELA 477
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
122-664 |
1.01e-16 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 84.83 E-value: 1.01e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVA-GHPYEEILQPLAQKLGLpclplpptnnLDALDETDTREK 200
Cdd:PRK12467 3159 IVALLAVLKAGGAYVPLDPEYPRERLAYMIEDSGVKLLLTqAHLLEQLPAPAGDTALT----------LDRLDLNGYSEN 3228
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 201 EITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIIlHTLPLHHLHGIVNKLLCPLWVGATCV 280
Cdd:PRK12467 3229 NPSTRVMGENLAYVIYTSGSTGKPKGVGVRHGALANHLCWIAEAYELDANDRV-LLFMSFSFDGAQERFLWTLICGGCLV 3307
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 281 MLPD--FQAQKVWEMLLsskAPTINVFMAVPTIYSKLIQFYDQHYQQPLlkefvkavckeriRLMVSGSAALPLPTLQRW 358
Cdd:PRK12467 3308 VRDNdlWDPEELWQAIH---AHRISIACFPPAYLQQFAEDAGGADCASL-------------DIYVFGGEAVPPAAFEQV 3371
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 359 K-EITGHTLLERYGMTEigmALSNPL----TGPRIPGS----VGVPLPGVDVRIVMNNkintsivegnh*ntwVRPGLEG 429
Cdd:PRK12467 3372 KrKLKPRGLTNGYGPTE---AVVTVTlwkcGGDAVCEApyapIGRPVAGRSIYVLDGQ---------------LNPVPVG 3433
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 430 KEGELLVRGQSVFKEYWNKpqetresftddgwfktgtePSLSSPAFVLTHFSAadvrtlidmlpgkgvnsaycsctirrC 509
Cdd:PRK12467 3434 VAGELYIGGVGLARGYHQR-------------------PSLTAERFVADPFSG--------------------------S 3468
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 510 RGTLPREercqtsfspmlqtgltptmt*ltslqrfahihplGDTAIYK-DGVYWIMGRTSVDIlKSGGFKISALEVECHL 588
Cdd:PRK12467 3469 GGRLYRT----------------------------------GDLARYRaDGVIEYLGRIDHQV-KIRGFRIELGEIEARL 3513
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 589 LAHPDIADVAVIGAPDATWGQKVTAVV----------QLRKGKSLTLPElqtwarehmgpYKIPTGLLLVEEMPRNQMGK 658
Cdd:PRK12467 3514 LQHPSVREAVVLARDGAGGKQLVAYVVpadpqgdwreTLRDHLAASLPD-----------YMVPAQLLVLAAMPLGPNGK 3582
|
....*.
gi 928020699 659 INKKDL 664
Cdd:PRK12467 3583 VDRKAL 3588
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
209-474 |
2.40e-15 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 79.40 E-value: 2.40e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTH----SSIQAMTQCL---------VSAWA-WSK----NDIIlhtlplhhlhGIVnkll 270
Cdd:cd05921 165 DTVAKFLFTSGSTGLPKAVINTQrmlcANQAMLEQTYpffgeeppvLVDWLpWNHtfggNHNF----------NLV---- 230
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 271 cpLWVGATcVMLPDFQ--AQKVWEML--LSSKAPTinVFMAVPTIYSKLIQFYDQhyQQPLLKEFVKavckeRIRLMVSG 346
Cdd:cd05921 231 --LYNGGT-LYIDDGKpmPGGFEETLrnLREISPT--VYFNVPAGWEMLVAALEK--DEALRRRFFK-----RLKLMFYA 298
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 347 SAALPLPTLQRWKEI----TGH--TLLERYGMTEIGmALSNPLTGPR-IPGSVGVPLPGVDVRIVMNNkintsivegnh* 419
Cdd:cd05921 299 GAGLSQDVWDRLQALavatVGEriPMMAGLGATETA-PTATFTHWPTeRSGLIGLPAPGTELKLVPSG------------ 365
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*
gi 928020699 420 ntwvrpgleGKEgELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGTEPSLSSPA 474
Cdd:cd05921 366 ---------GKY-EVRVKGPNVTPGYWRQPELTAQAFDEEGFYCLGDAAKLADPD 410
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
80-664 |
4.44e-15 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 78.51 E-value: 4.44e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLV 159
Cdd:PRK13390 26 SYRQLDDDSAALARVLYDA-----GLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAPEADYIVGDSGARVL 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 160 VAGHPYEEILqplAQKLGLPCLPLPPTNNLDALDETdtrekEITITD----WTDRP--AMIIYTSGTTGRPKGV---LHT 230
Cdd:PRK13390 101 VASAALDGLA---AKVGADLPLRLSFGGEIDGFGSF-----EAALAGagprLTEQPcgAVMLYSSGTTGFPKGIqpdLPG 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 231 HSSIQAMTQCLVSAWAW---SKNDIILHTLPLHHLH-----GIVNKLlcplwvGATCVMLPDFQAQkvwEMLLSSKAPTI 302
Cdd:PRK13390 173 RDVDAPGDPIVAIARAFydiSESDIYYSSAPIYHAAplrwcSMVHAL------GGTVVLAKRFDAQ---ATLGHVERYRI 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 303 NVFMAVPTIYSKLIQFYDQ---HYQQPLLKEFVKAvckerirlmvsgSAALPLPTLQRWKEITGHTLLERYGMTEI-GMA 378
Cdd:PRK13390 244 TVTQMVPTMFVRLLKLDADvrtRYDVSSLRAVIHA------------AAPCPVDVKHAMIDWLGPIVYEYYSSTEAhGMT 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 379 LSNPLTGPRIPGSVGVPLPGvDVRIVMNnkintsivEGNH*ntwvrPGleGKEGELLVRGQSVFKEYWNKPQETRESftd 458
Cdd:PRK13390 312 FIDSPDWLAHPGSVGRSVLG-DLHICDD--------DGNEL-----PA--GRIGTVYFERDRLPFRYLNDPEKTAAA--- 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 459 dgwfktgtepslSSPAfvlthfsaadvrtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfSPMLQTgltptmt*l 538
Cdd:PRK13390 373 ------------QHPA-------------------------------------------------HPFWTT--------- 382
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 539 tslqrfahihpLGDTA-IYKDGVYWIMGRTSVDILkSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQL 617
Cdd:PRK13390 383 -----------VGDLGsVDEDGYLYLADRKSFMII-SGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIQL 450
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|
gi 928020699 618 RKG--KSLTLP-ELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK13390 451 VEGirGSDELArELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLL 500
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
80-659 |
5.49e-15 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 78.30 E-value: 5.49e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYCSSlglaSRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLV 159
Cdd:cd05968 93 TYGELLYEV----KRLANGL-RALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATRLQDAEAKAL 167
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 160 VAG----------HPYEEILQPLAQ----------KLGLPCLPLPPTNNLDALDETDTREKEITITDWTDrPAMIIYTSG 219
Cdd:cd05968 168 ITAdgftrrgrevNLKEEADKACAQcptvekvvvvRHLGNDFTPAKGRDLSYDEEKETAGDGAERTESED-PLMIIYTSG 246
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 220 TTGRPKGVLHTHS-----SIQAMTQCL----------VSAWAWSKNDIIlhtlplhhlhgivnkLLCPLWVGATCVM--- 281
Cdd:cd05968 247 TTGKPKGTVHVHAgfplkAAQDMYFQFdlkpgdlltwFTDLGWMMGPWL---------------IFGGLILGATMVLydg 311
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 282 LPDF-QAQKVWEMLLSSKAPTINVfmaVPTIYSKLIQFYDqhyqqpllkEFVKAvcKERIRLMVSGSAALPLpTLQRWKE 360
Cdd:cd05968 312 APDHpKADRLWRMVEDHEITHLGL---SPTLIRALKPRGD---------APVNA--HDLSSLRVLGSTGEPW-NPEPWNW 376
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 361 ITGHTLLER-----Y-GMTEI-GMALSNPLTGPRIPGSVGVPLPGVDVRIVmnnkintsivegNH*NTWVRPglegKEGE 433
Cdd:cd05968 377 LFETVGKGRnpiinYsGGTEIsGGILGNVLIKPIKPSSFNGPVPGMKADVL------------DESGKPARP----EVGE 440
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 434 LLVRGQsvfkeyWnkPQETRESFTDDGWFktgtepslsspafvlthfsaadVRTLIDMLPGKGVNsaycsctirrcrgtl 513
Cdd:cd05968 441 LVLLAP------W--PGMTRGFWRDEDRY----------------------LETYWSRFDNVWVH--------------- 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 514 preercqtsfspmlqtgltptmt*ltslqrfahihplGDTAIY-KDGVYWIMGRtSVDILKSGGFKISALEVECHLLAHP 592
Cdd:cd05968 476 -------------------------------------GDFAYYdEEGYFYILGR-SDDTINVAGKRVGPAEIESVLNAHP 517
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 593 DIADVAVIGAPDATWGQKVTAVVQLRKGKSLT---LPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKI 659
Cdd:cd05968 518 AVLESAAIGVPHPVKGEAIVCFVVLKPGVTPTealAEELMERVADELGKPLSPERILFVKDLPKTRNAKV 587
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
209-465 |
9.47e-15 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 77.78 E-value: 9.47e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIV--NKLLCP-LWVGATCVM---- 281
Cdd:PRK12582 220 DTVAKYLFTSGSTGMPKAVINTQRMMCANIAMQEQLRPREPDPPPPVSLDWMPWNHTMggNANFNGlLWGGGTLYIddgk 299
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 282 -LPDFQAQKVWEmlLSSKAPTinVFMAVPTIYSKLIQFYDQhyQQPLLKEFVKavckeRIRLMVSGSAALPLPTLQRWKE 360
Cdd:PRK12582 300 pLPGMFEETIRN--LREISPT--VYGNVPAGYAMLAEAMEK--DDALRRSFFK-----NLRLMAYGGATLSDDLYERMQA 368
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 361 ----ITGH--TLLERYGMTEIGMALSNPLTGPRIPGSVGVPLPGVDVRIVMNnkintsivegnh*ntwvrpgleGKEGEL 434
Cdd:PRK12582 369 lavrTTGHriPFYTGYGATETAPTTTGTHWDTERVGLIGLPLPGVELKLAPV----------------------GDKYEV 426
|
250 260 270
....*....|....*....|....*....|.
gi 928020699 435 LVRGQSVFKEYWNKPQETRESFTDDGWFKTG 465
Cdd:PRK12582 427 RVKGPNVTPGYHKDPELTAAAFDEEGFYRLG 457
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
206-666 |
9.83e-15 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 77.63 E-value: 9.83e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 206 DWTDR--PAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVS-AWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVML 282
Cdd:PLN02654 270 EWVDAedPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKyAFDYKPTDVYWCTADCGWITGHSYVTYGPMLNGATVLVF 349
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 283 ---PDF-QAQKVWEMLLSSKaptINVFMAVPTIYSKLIQFYDqhyqqpllkEFVKAVCKERIRLMvsGSAALPL-PTLQR 357
Cdd:PLN02654 350 egaPNYpDSGRCWDIVDKYK---VTIFYTAPTLVRSLMRDGD---------EYVTRHSRKSLRVL--GSVGEPInPSAWR 415
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 358 W-KEITGHT---LLERYGMTEIGMALSNPLTG--PRIPGSVGVPLPGVDVRIVMNNkintsivegnh*ntwvrpgleGKE 431
Cdd:PLN02654 416 WfFNVVGDSrcpISDTWWQTETGGFMITPLPGawPQKPGSATFPFFGVQPVIVDEK---------------------GKE 474
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 432 GELLVRGQSVFKEYWnkPQETRESFTDDGWFKTgtepslsspafvlthfsaadvrtlidmlpgkgvnsaycsctirrcrg 511
Cdd:PLN02654 475 IEGECSGYLCVKKSW--PGAFRTLYGDHERYET----------------------------------------------- 505
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 512 tlpreercqTSFSPmlqtgltptmt*ltslqrFAHIHPLGDTAIY-KDGVYWIMGRTSvDILKSGGFKISALEVECHLLA 590
Cdd:PLN02654 506 ---------TYFKP------------------FAGYYFSGDGCSRdKDGYYWLTGRVD-DVINVSGHRIGTAEVESALVS 557
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 591 HPDIADVAVIGAPDATWGQKVTAVVQLRKG--------KSLTLPelqtwAREHMGPYKIPTGLLLVEEMPRNQMGKINKK 662
Cdd:PLN02654 558 HPQCAEAAVVGIEHEVKGQGIYAFVTLVEGvpyseelrKSLILT-----VRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 632
|
....
gi 928020699 663 DLLK 666
Cdd:PLN02654 633 ILRK 636
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
111-664 |
1.22e-14 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 77.37 E-value: 1.22e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 111 ISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYE----EILQPLAQKLGLPCLPLPPT 186
Cdd:PLN03102 67 VSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFVDRSFEplarEVLHLLSSEDSNLNLPVIFI 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 187 NNLDA--------LD-ETDTREKEIT---------ITDWTDrPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWaws 248
Cdd:PLN03102 147 HEIDFpkrpsseeLDyECLIQRGEPTpslvarmfrIQDEHD-PISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGW--- 222
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 249 kndiilhtlplhhLHGIvnkllCP--LWVgatcvmLPDFQAQKvWEMLLSS-------------KAPTI--NVFM----- 306
Cdd:PLN03102 223 -------------EMGT-----CPvyLWT------LPMFHCNG-WTFTWGTaarggtsvcmrhvTAPEIykNIEMhnvth 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 307 --AVPTIYSKLIQ--FYDQHYQQPllkefvkavckeRIRLMVSGSAalPLPTLQRWKEITGHTLLERYGMTEIgmalsnp 382
Cdd:PLN03102 278 mcCVPTVFNILLKgnSLDLSPRSG------------PVHVLTGGSP--PPAALVKKVQRLGFQVMHAYGLTEA------- 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 383 lTGP-----------RIP-----------GSVGVPLPGVDVRivmNNKINTSIVEgnh*ntwvrpglEGKE-GELLVRGQ 439
Cdd:PLN03102 337 -TGPvlfcewqdewnRLPenqqmelkarqGVSILGLADVDVK---NKETQESVPR------------DGKTmGEIVIKGS 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 440 SVFKEYWNKPQETRESFTDdGWFKTGtepslsspafvlthfsaaDVrtlidmlpgkGVnsaycsctirrcrgtlpreerc 519
Cdd:PLN03102 401 SIMKGYLKNPKATSEAFKH-GWLNTG------------------DV----------GV---------------------- 429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 520 qtsfspmlqtgltptmt*ltslqrfahIHPLGDTAIyKDgvywimgrTSVDILKSGGFKISALEVECHLLAHPDIADVAV 599
Cdd:PLN03102 430 ---------------------------IHPDGHVEI-KD--------RSKDIIISGGENISSVEVENVLYKYPKVLETAV 473
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 928020699 600 IGAPDATWGQKVTAVVQLRKGKS---LTLPELQT-------WAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PLN03102 474 VAMPHPTWGETPCAFVVLEKGETtkeDRVDKLVTrerdlieYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKL 548
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
208-465 |
2.93e-14 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 76.09 E-value: 2.93e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 208 TDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIilhtlplhhlhgivnKLLC-PLWV------GATCV 280
Cdd:PRK09274 173 PDDMAAILFTSGSTGTPKGVVYTHGMFEAQIEALREDYGIEPGEI---------------DLPTfPLFAlfgpalGMTSV 237
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 281 mLPDFQAqkvwemllsSKAPTINvfmavPtiySKLIQFYDQhYQ------QPLLKEFVKAVCKER------IRLMVSGSA 348
Cdd:PRK09274 238 -IPDMDP---------TRPATVD-----P---AKLFAAIER-YGvtnlfgSPALLERLGRYGEANgiklpsLRRVISAGA 298
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 349 ALPLPTLQRWKEITGHT--LLERYGMTE------IGMalSNPLTGPRI-----PGS-VGVPLPGVDVRIVmnnkintSIV 414
Cdd:PRK09274 299 PVPIAVIERFRAMLPPDaeILTPYGATEalpissIES--REILFATRAatdngAGIcVGRPVDGVEVRII-------AIS 369
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*..
gi 928020699 415 EGNh*NTW--VRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDDG----WFKTG 465
Cdd:PRK09274 370 DAP-IPEWddALRLATGEIGEIVVAGPMVTRSYYNRPEATRLAKIPDGqgdvWHRMG 425
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
41-666 |
5.97e-14 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 75.02 E-value: 5.97e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 41 RNSHRSALAPSWVEKQKPVFTKAPEFGEKLAVIDSCGKHSYKqlYCSSLGLASRISSALGSdFGGLDGKRISFLCANDSS 120
Cdd:PLN02330 16 RSRYPSVPVPDKLTLPDFVLQDAELYADKVAFVEAVTGKAVT--YGEVVRDTRRFAKALRS-LGLRKGQVVVVVLPNVAE 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 121 YVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHP-YEEI----LQPLAQKLGLPCLPLPPTNNLDALDE- 194
Cdd:PLN02330 93 YGIVALGIMAAGGVFSGANPTALESEIKKQAEAAGAKLIVTNDTnYGKVkglgLPVIVLGEEKIEGAVNWKELLEAADRa 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 195 TDTRE-KEITITDWTDRPamiiYTSGTTGRPKGVLHTHSSIQAMtqcLVSAWAWSKNDIILHTLPLHHL-----HGIVNK 268
Cdd:PLN02330 173 GDTSDnEEILQTDLCALP----FSSGTTGISKGVMLTHRNLVAN---LCSSLFSVGPEMIGQVVTLGLIpffhiYGITGI 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 269 LLCPLWVGATCVMLPDFQAQKVWEMLLS---SKAPTinvfmaVPTIYSKLIQfydqhyqQPLLKEFvkAVCKERIRLMVS 345
Cdd:PLN02330 246 CCATLRNKGKVVVMSRFELRTFLNALITqevSFAPI------VPPIILNLVK-------NPIVEEF--DLSKLKLQAIMT 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 346 GSAALPLPTLQRW-KEITGHTLLERYGMTE---IGMALSNPLTGPRIP--GSVGVPLPGVDVRIVmNNKINTSIVEgnh* 419
Cdd:PLN02330 311 AAAPLAPELLTAFeAKFPGVQVQEAYGLTEhscITLTHGDPEKGHGIAkkNSVGFILPNLEVKFI-DPDTGRSLPK---- 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 420 NTwvrpglegkEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTGtepslsspafvlthfsaaDVRTLIDmlpgkgvns 499
Cdd:PLN02330 386 NT---------PGELCVRSQCVMQGYYNNKEETDRTIDEDGWLHTG------------------DIGYIDD--------- 429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 500 aycsctirrcrgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYWIMGRTSvDILKSGGFKI 579
Cdd:PLN02330 430 ----------------------------------------------------------DGDIFIVDRIK-ELIKYKGFQV 450
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 580 SALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKI 659
Cdd:PLN02330 451 APAELEAILLTHPSVEDAAVVPLPDEEAGEIPAACVVINPKAKESEEDILNFVAANVAHYKKVRVVQFVDSIPKSLSGKI 530
|
....*..
gi 928020699 660 NKKdLLK 666
Cdd:PLN02330 531 MRR-LLK 536
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
46-664 |
1.03e-13 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 74.27 E-value: 1.03e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 46 SALAPSWVEKqkpVFTKAPEFGEKLAV--IDSCGKHSYKQLYCSSLGLASRISSALGSDfggldGKRISFLCANDSSYVV 123
Cdd:PRK05857 10 PQLPSTVLDR---VFEQARQQPEAIALrrCDGTSALRYRELVAEVGGLAADLRAQSVSR-----GSRVLVISDNGPETYL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 124 AQWAVWMSGGTAVPLYRKHPPSELEYI--ISDSQSSLVVAGHPYEEILQPLAQKLGLPCLPLPPTNNLDALDETDTREKE 201
Cdd:PRK05857 82 SVLACAKLGAIAVMADGNLPIAAIERFcqITDPAAALVAPGSKMASSAVPEALHSIPVIAVDIAAVTRESEHSLDAASLA 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 202 ITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVS---AWA-WSKNDIILHTLPLHHLHGIVNKLLCpLWVGA 277
Cdd:PRK05857 162 GNADQGSEDPLAMIFTSGTTGEPKAVLLANRTFFAVPDILQKeglNWVtWVVGETTYSPLPATHIGGLWWILTC-LMHGG 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 278 TCVMLPDfQAQKVWEMLLSSKAPTINVfmaVPTIYSKLIqfydqhYQQPLLKEFVKAvckerIRLMV-SGSAALPLPTlq 356
Cdd:PRK05857 241 LCVTGGE-NTTSLLEILTTNAVATTCL---VPTLLSKLV------SELKSANATVPS-----LRLVGyGGSRAIAADV-- 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 357 RWKEITGHTLLERYGMTEIG-MALSNPLTGPRIP----GSVGVPLPGVDVRIVMNNKINTSIVEGNH*NTWvrpglegke 431
Cdd:PRK05857 304 RFIEATGVRTAQVYGLSETGcTALCLPTDDGSIVkieaGAVGRPYPGVDVYLAATDGIGPTAPGAGPSASF--------- 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 432 GELLVRGQSVFKEYWNKPQETRESFTdDGWFKTGtepslsspafvlthfsaadvrtliDMLpgkgvnsaycsctirrcrg 511
Cdd:PRK05857 375 GTLWIKSPANMLGYWNNPERTAEVLI-DGWVNTG------------------------DLL------------------- 410
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 512 tlpreERcqtsfspmlqtgltptmt*ltslqrfahihplgdtaiYKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAH 591
Cdd:PRK05857 411 -----ER-------------------------------------REDGFFYIKGRSS-EMIICGGVNIAPDEVDRIAEGV 447
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 928020699 592 PDIADVAVIGAPDATWGQKV-TAVVQLRKGKSLTLPELQT-----WAREHMGPYKiPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK05857 448 SGVREAACYEIPDEEFGALVgLAVVASAELDESAARALKHtiaarFRRESEPMAR-PSTIVIVTDIPRTQSGKVMRASL 525
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
108-465 |
2.34e-13 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 73.26 E-value: 2.34e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 108 GKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQ------------------------SSLVVAGH 163
Cdd:cd17632 93 GDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEprllavsaehldlaveavleggtpPRLVVFDH 172
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 164 PYE-----EILQPlAQKLGLPCLPLPPTNNLDALDETDTREKEITITD-WTDRPAMIIYTSGTTGRPKGVLHTHSsiqam 237
Cdd:cd17632 173 RPEvdahrAALES-ARERLAAVGIPVTTLTLIAVRGRDLPPAPLFRPEpDDDPLALLIYTSGSTGTPKGAMYTER----- 246
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 238 tqcLVsAWAWSKNDIIlhtLPLHHLHGIV------------NKLLCPLWVGATCVMLPDFQAQKVWEMlLSSKAPTiNVF 305
Cdd:cd17632 247 ---LV-ATFWLKVSSI---QDIRPPASITlnfmpmshiagrISLYGTLARGGTAYFAAASDMSTLFDD-LALVRPT-ELF 317
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 306 MaVPTIYSKLIqfydQHYQQPL------------LKEFVKAVCKERI---RLM--VSGSAALPlPTLQRWKEIT-GHTLL 367
Cdd:cd17632 318 L-VPRVCDMLF----QRYQAELdrrsvagadaetLAERVKAELRERVlggRLLaaVCGSAPLS-AEMKAFMESLlDLDLH 391
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 368 ERYGMTEIGMALSNpltgpripGSVGVPlPGVDVRIVmnnkintSIVEGNH*NTwVRPgleGKEGELLVRGQSVFKEYWN 447
Cdd:cd17632 392 DGYGSTEAGAVILD--------GVIVRP-PVLDYKLV-------DVPELGYFRT-DRP---HPRGELLVKTDTLFPGYYK 451
|
410
....*....|....*...
gi 928020699 448 KPQETRESFTDDGWFKTG 465
Cdd:cd17632 452 RPEVTAEVFDEDGFYRTG 469
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
117-465 |
9.24e-13 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 71.23 E-value: 9.24e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 117 NDSSYVVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAG--------HPYEEILQPLAQKLGLPCLPLPPTNN 188
Cdd:cd05933 42 NSPEWFIAAVGAIFAGGIAVGIYTTNSPEACQYVAETSEANILVVEnqkqlqkiLQIQDKLPHLKAIIQYKEPLKEKEPN 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 189 L--------DALDETDTREKEITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAwawskndiILHTLPLH 260
Cdd:cd05933 122 LyswdefmeLGRSIPDEQLDAIISSQKPNQCCTLIYTSGTTGMPKGVMLSHDNITWTAKAASQH--------MDLRPATV 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 261 HLHGIVNKLlcPL----------WVGAT---CVMLPDFQAQKvWEM--LLSSKAPTInvFMAVPTIYSKLIQ-------- 317
Cdd:cd05933 194 GQESVVSYL--PLshiaaqildiWLPIKvggQVYFAQPDALK-GTLvkTLREVRPTA--FMGVPRVWEKIQEkmkavgak 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 318 --FYDQ--------------------HYQQPLLKEFVKAVCKERIRLMV---------SGSAALPLPTLQRWKEITgHTL 366
Cdd:cd05933 269 sgTLKRkiaswakgvgletnlklmggESPSPLFYRLAKKLVFKKVRKALgldrcqkffTGAAPISRETLEFFLSLN-IPI 347
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 367 LERYGMTEIGMA--LSNPlTGPRIpGSVGVPLPGVDVRIVMNNKintsivEGNh*ntwvrpglegkeGELLVRGQSVFKE 444
Cdd:cd05933 348 MELYGMSETSGPhtISNP-QAYRL-LSCGKALPGCKTKIHNPDA------DGI--------------GEICFWGRHVFMG 405
|
410 420
....*....|....*....|.
gi 928020699 445 YWNKPQETRESFTDDGWFKTG 465
Cdd:cd05933 406 YLNMEDKTEEAIDEDGWLHSG 426
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
122-664 |
1.84e-12 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 70.97 E-value: 1.84e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVA-GHPYEEILQPlaqklglpclPLPPTNNLDALDetdtrek 200
Cdd:PRK05691 1195 LVGLLAILKAGGAYVPLDPDYPAERLAYMLADSGVELLLTqSHLLERLPQA----------EGVSAIALDSLH------- 1257
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 201 eitITDWTDRP----------AMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGiVNKLL 270
Cdd:PRK05691 1258 ---LDSWPSQApglhlhgdnlAYVIYTSGSTGQPKGVGNTHAALAERLQWMQATYALDDSDVLMQKAPISFDVS-VWECF 1333
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 271 CPLWVGATCVMLPDFQ---AQKVWEMLLSSKAPTInvfmavptiyskliqfydqHYQQPLLKEFVK----AVCKeRIRLM 343
Cdd:PRK05691 1334 WPLITGCRLVLAGPGEhrdPQRIAELVQQYGVTTL-------------------HFVPPLLQLFIDeplaAACT-SLRRL 1393
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 344 VSGSAALPLP----TLQRWKEITGHTlleRYGMTEIGMALSN----PLTGPRIPgsVGVPLPGVDVRiVMNNKINtsive 415
Cdd:PRK05691 1394 FSGGEALPAElrnrVLQRLPQVQLHN---RYGPTETAINVTHwqcqAEDGERSP--IGRPLGNVLCR-VLDAELN----- 1462
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 416 gnh*ntwvrPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDDgwfktgtepslsspafvlthfsaadvrtlidmlpgk 495
Cdd:PRK05691 1463 ---------LLPPGVAGELCIGGAGLARGYLGRPALTAERFVPD------------------------------------ 1497
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 496 gvnsaycsctirrcrgtlpreercqtsfsPMLQTGltptmt*ltslqrfAHIHPLGDTAIYK-DGVYWIMGRTSVDIlKS 574
Cdd:PRK05691 1498 -----------------------------PLGEDG--------------ARLYRTGDRARWNaDGALEYLGRLDQQV-KL 1533
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 575 GGFKISALEVECHLLAHPDIADVAVIGAPDATwGQKVTAVVQLRKGKSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRN 654
Cdd:PRK05691 1534 RGFRVEPEEIQARLLAQPGVAQAAVLVREGAA-GAQLVGYYTGEAGQEAEAERLKAALAAELPEYMVPAQLIRLDQMPLG 1612
|
570
....*....|
gi 928020699 655 QMGKINKKDL 664
Cdd:PRK05691 1613 PSGKLDRRAL 1622
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
68-664 |
2.54e-12 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 69.43 E-value: 2.54e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSSLGLASRISSAlgsdfgGLDGKRISFLCANDS-SYVVAQWAVWMSGGTAVPLYRKHPPSE 146
Cdd:cd17656 3 DAVAVVFENQKLTYRELNERSNQLARFLREK------GVKKDSIVAIMMERSaEMIVGILGILKAGGAFVPIDPEYPEER 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 147 LEYIISDSQSSLVVAghpYEEILQPLAQKLGlpclplppTNNLDALDETDTREKEITITDWTDRPAMIIYTSGTTGRPKG 226
Cdd:cd17656 77 RIYIMLDSGVRVVLT---QRHLKSKLSFNKS--------TILLEDPSISQEDTSNIDYINNSDDLLYIIYTSGTTGKPKG 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 227 VLHTHSSI-----------QAMTQCLVSAWAWSKNDIILHTlplhhlhgIVNKLLcplwVGATCVMLPDFQAQKVWEML- 294
Cdd:cd17656 146 VQLEHKNMvnllhferektNINFSDKVLQFATCSFDVCYQE--------IFSTLL----SGGTLYIIREETKRDVEQLFd 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 295 LSSKAPTINVFMavPTIYSKLIqFYDQHYQQPLLKEfVKAVCKERIRLMVSGsaalplpTLQRWKEITGHTLLERYGMTE 374
Cdd:cd17656 214 LVKRHNIEVVFL--PVAFLKFI-FSEREFINRFPTC-VKHIITAGEQLVITN-------EFKEMLHEHNVHLHNHYGPSE 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 375 ---IGMALSNPltGPRIP--GSVGVPLPGVDVRIVMNNKintsivegnh*ntwvRPGLEGKEGELLVRGQSVFKEYWNKP 449
Cdd:cd17656 283 thvVTTYTINP--EAEIPelPPIGKPISNTWIYILDQEQ---------------QLQPQGIVGELYISGASVARGYLNRQ 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 450 QETRESFTDDGwFKtgtepslsspafvlthfsaADVRtlidmlpgkgvnsaycsctirrcrgtlpreercqtsfspMLQT 529
Cdd:cd17656 346 ELTAEKFFPDP-FD-------------------PNER---------------------------------------MYRT 366
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 530 gltptmt*ltslqrfahihplGDTAIY-KDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWG 608
Cdd:cd17656 367 ---------------------GDLARYlPDGNIEFLGRAD-HQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGE 424
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*..
gi 928020699 609 QKVTA-VVQLRKgksLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd17656 425 KYLCAyFVMEQE---LNISQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
209-476 |
2.82e-12 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 70.12 E-value: 2.82e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQ 288
Cdd:PRK08043 365 EDAALILFTSGSEGHPKGVVHSHKSLLANVEQIKTIADFTPNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHY 444
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 289 KVWEMLLSSKAPTinVFMAVPTI---YSKLIQFYDQHyqqpllkefvkavckeRIRLMVSGSAALPLPTLQRWKEITGHT 365
Cdd:PRK08043 445 RIVPELVYDRNCT--VLFGTSTFlgnYARFANPYDFA----------------RLRYVVAGAEKLQESTKQLWQDKFGLR 506
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 366 LLERYGMTEIG--MALSNPLTGPriPGSVGVPLPGVDVRIVmnnkintsivegnh*ntwVRPGLEgKEGELLVRGQSVFK 443
Cdd:PRK08043 507 ILEGYGVTECApvVSINVPMAAK--PGTVGRILPGMDARLL------------------SVPGIE-QGGRLQLKGPNIMN 565
|
250 260 270 280
....*....|....*....|....*....|....*....|..
gi 928020699 444 EY--------WNKPQ-ETRESFTDDGWFKTGTEPSLSSPAFV 476
Cdd:PRK08043 566 GYlrvekpgvLEVPTaENARGEMERGWYDTGDIVRFDEQGFV 607
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
72-666 |
3.90e-12 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 69.15 E-value: 3.90e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 72 VIDSCG-KHSYKQLYCSSLGLASRISSALGSD------FGGLDGKRI-SFLCANDS--SYV-VAQwavwmsggtavplyr 140
Cdd:PRK04813 20 AYDYLGeKLTYGQLKEDSDALAAFIDSLKLPDkspiivFGHMSPEMLaTFLGAVKAghAYIpVDV--------------- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 141 kHPPSE-LEYIISDSQSSLVVAGHPYEEILQPLaqklglpclplpPTNNLDALDETDTREKEITITDWT--DRPAMIIYT 217
Cdd:PRK04813 85 -SSPAErIEMIIEVAKPSLIIATEELPLEILGI------------PVITLDELKDIFATGNPYDFDHAVkgDDNYYIIFT 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 218 SGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCpLWVGATCVMLP-----DFqaQKVWE 292
Cdd:PRK04813 152 SGTTGKPKGVQISHDNLVSFTNWMLEDFALPEGPQFLNQAPYSFDLSVMDLYPT-LASGGTLVALPkdmtaNF--KQLFE 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 293 MLLSSKaptINVFMAVPTiyskLIQ-------FYDQHYqqPLLKEFVkaVCKErirlmvsgsaALPLPTLQrwkeitghT 365
Cdd:PRK04813 229 TLPQLP---INVWVSTPS----FADmclldpsFNEEHL--PNLTHFL--FCGE----------ELPHKTAK--------K 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 366 LLER---------YGMTEIGMALSN-PLT------GPRIPgsVGVPLPGVDVRIVMNNkintsiveGNh*ntwvrPGLEG 429
Cdd:PRK04813 280 LLERfpsatiyntYGPTEATVAVTSiEITdemldqYKRLP--IGYAKPDSPLLIIDEE--------GT-------KLPDG 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 430 KEGELLVRGQSVFKEYWNKPQETRESF-TDDGwfktgtepslsSPAFvlthfsaadvRTlidmlpgkgvnsaycsctirr 508
Cdd:PRK04813 343 EQGEIVISGPSVSKGYLNNPEKTAEAFfTFDG-----------QPAY----------HT--------------------- 380
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 509 crgtlpreercqtsfspmlqtgltptmt*ltslqrfahihplGDTAIYKDGVYWIMGRTSVDIlKSGGFKISALEVECHL 588
Cdd:PRK04813 381 ------------------------------------------GDAGYLEDGLLFYQGRIDFQI-KLNGYRIELEEIEQNL 417
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 589 LAHPDIAD-VAVIGAPDatwgQKVT---AVVQLRKG---KSLTLP-----ELQtwarEHMGPYKIPTGLLLVEEMPRNQM 656
Cdd:PRK04813 418 RQSSYVESaVVVPYNKD----HKVQyliAYVVPKEEdfeREFELTkaikkELK----ERLMEYMIPRKFIYRDSLPLTPN 489
|
650
....*....|
gi 928020699 657 GKINKKDLLK 666
Cdd:PRK04813 490 GKIDRKALIE 499
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
68-664 |
8.14e-12 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 67.96 E-value: 8.14e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 68 EKLAVIDSCGKHSYKQLYCSslglASRISSALgSDFGGLDGKRISFLCANDSSYVVAQWAVWMSGGTAVPLYRKHPPSEL 147
Cdd:cd17645 13 DHVAVVDRGQSLTYKQLNEK----ANQLARHL-RGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYPGERI 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 148 EYIISDSQSSLVvaghpyeeilqplaqklglpclplpptnnldaldetdtrekeitITDwTDRPAMIIYTSGTTGRPKGV 227
Cdd:cd17645 88 AYMLADSSAKIL--------------------------------------------LTN-PDDLAYVIYTSGSTGLPKGV 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 228 LHTHSSIQAMTQCLVSAWAWSKNDiilhtlPLHHLHGIvnkllcplwvgatcvmlpDFQAQkVWEMLlsskaPTINVFMA 307
Cdd:cd17645 123 MIEHHNLVNLCEWHRPYFGVTPAD------KSLVYASF------------------SFDAS-AWEIF-----PHLTAGAA 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 308 VPTIYS-------KLIQFYDQHY------QQPLLKEFVkAVCKERIRLMVSGSaalplPTLQRWKEiTGHTLLERYGMTE 374
Cdd:cd17645 173 LHVVPSerrldldALNDYFNQEGitisflPTGAAEQFM-QLDNQSLRVLLTGG-----DKLKKIER-KGYKLVNNYGPTE 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 375 IG-MALSNPLTGPRIPGSVGVPLPGVDVRIvmnnkintsIVEGNH*ntwVRPglEGKEGELLVRGQSVFKEYWNKPQETR 453
Cdd:cd17645 246 NTvVATSFEIDKPYANIPIGKPIDNTRVYI---------LDEALQ----LQP--IGVAGELCIAGEGLARGYLNRPELTA 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 454 ESFTDDgwfktgtepslsspAFVlthfsaadvrtlidmlPGkgvnsaycsctirrcrgtlpreERcqtsfspMLQTGltp 533
Cdd:cd17645 311 EKFIVH--------------PFV----------------PG----------------------ER-------MYRTG--- 328
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 534 tmt*ltSLQRFAhihplgdtaiyKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTA 613
Cdd:cd17645 329 ------DLAKFL-----------PDGNIEFLGRLD-QQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGRKYLVA 390
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|.
gi 928020699 614 VVQLRKgkSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd17645 391 YVTAPE--EIPHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
209-664 |
9.58e-12 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 67.78 E-value: 9.58e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAq 288
Cdd:PRK07867 152 DDLFMLIFTSGTSGDPKAVRCTHRKVASAGVMLAQRFGLGPDDVCYVSMPLFHSNAVMAGWAVALAAGASIALRRKFSA- 230
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 289 kvwemllSSKAPTINVFMAVptiyskliqfYDQHYQQPLlkEFVKAVcKER-------IRLMVsGSAALPlPTLQRWKEI 361
Cdd:PRK07867 231 -------SGFLPDVRRYGAT----------YANYVGKPL--SYVLAT-PERpddadnpLRIVY-GNEGAP-GDIARFARR 288
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 362 TGHTLLERYGMTEIGMALSNPLTGPriPGSVGVPLPGVDVRivmNNKINTSIVEGNH*NTWVRPGLEGKeGELL-VRGQS 440
Cdd:PRK07867 289 FGCVVVDGFGSTEGGVAITRTPDTP--PGALGPLPPGVAIV---DPDTGTECPPAEDADGRLLNADEAI-GELVnTAGPG 362
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 441 VFKEYWNKPQETRESFTDdGWFKTGtepslsspafvlthfsaadvrtliDMlpgkgvnsAYcsctirrcrgtlpREErcq 520
Cdd:PRK07867 363 GFEGYYNDPEADAERMRG-GVYWSG------------------------DL--------AY-------------RDA--- 393
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 521 tsfspmlqtgltptmt*ltslqrfahihplgdtaiykDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVI 600
Cdd:PRK07867 394 -------------------------------------DGYAYFAGRLG-DWMRVDGENLGTAPIERILLRYPDATEVAVY 435
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 928020699 601 GAPDATWGQKVTAVVQLRKGKSLTLPELQTW--AREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK07867 436 AVPDPVVGDQVMAALVLAPGAKFDPDAFAEFlaAQPDLGPKQWPSYVRVCAELPRTATFKVLKRQL 501
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
53-465 |
1.41e-11 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 67.74 E-value: 1.41e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 53 VEKqkpvFTKAPEFGEKLAVIDSCGKH---SYKQLYCSSLGLASRISSAlgsdfGGLDGKRISFLCANDSSYVVAQWAVW 129
Cdd:PLN02614 55 VEK----YPNNPMLGRREIVDGKPGKYvwqTYQEVYDIVIKLGNSLRSV-----GVKDEAKCGIYGANSPEWIISMEACN 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 130 MSGGTAVPLYRKHPPSELEYIISDSQSSLVVAghpYEEILQPLAQKLGLPCLPLPPTNNLDALDETDTREKE---ITITD 206
Cdd:PLN02614 126 AHGLYCVPLYDTLGAGAVEFIISHSEVSIVFV---EEKKISELFKTCPNSTEYMKTVVSFGGVSREQKEEAEtfgLVIYA 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 207 WTD---------------RPA---MIIYTSGTTGRPKGVLHTHSSIQAMTQCLV-----SAWAWSKNDIILHTLPLHHlh 263
Cdd:PLN02614 203 WDEflklgegkqydlpikKKSdicTIMYTSGTTGDPKGVMISNESIVTLIAGVIrllksANAALTVKDVYLSYLPLAH-- 280
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 264 gIVNKLL--CPLWVGATCvmlpDFQAQKVWEML--LSSKAPTInvFMAVPTI----YSKLIQ-----------FYDQHYQ 324
Cdd:PLN02614 281 -IFDRVIeeCFIQHGAAI----GFWRGDVKLLIedLGELKPTI--FCAVPRVldrvYSGLQKklsdggflkkfVFDSAFS 353
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 325 ---------------QPLLKEFVKAVCKE----RIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTE--IGMALSNPl 383
Cdd:PLN02614 354 ykfgnmkkgqshveaSPLCDKLVFNKVKQglggNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTEscAGTFVSLP- 432
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 384 TGPRIPGSVGVPLPGVDVRIvmnnkinTSIVEGNH*NTWVRPglegkEGELLVRGQSVFKEYWNKPQETRESFTdDGWFK 463
Cdd:PLN02614 433 DELDMLGTVGPPVPNVDIRL-------ESVPEMEYDALASTP-----RGEICIRGKTLFSGYYKREDLTKEVLI-DGWLH 499
|
..
gi 928020699 464 TG 465
Cdd:PLN02614 500 TG 501
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
209-465 |
3.62e-11 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 65.94 E-value: 3.62e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWawskndiilhtlplhhlhGIVnkllcplwvgATCVMLPDFQAQ 288
Cdd:cd05910 85 DEPAAILFTSGSTGTPKGVVYRHGTFAAQIDALRQLY------------------GIR----------PGEVDLATFPLF 136
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 289 KVWEMLL--SSKAPTINVFMAVPTIYSKLIQFYDQH-----YQQPLLKEFVKAVCKER------IRLMVSGSAALPLPTL 355
Cdd:cd05910 137 ALFGPALglTSVIPDMDPTRPARADPQKLVGAIRQYgvsivFGSPALLERVARYCAQHgitlpsLRRVLSAGAPVPIALA 216
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 356 QRWKEITGHT--LLERYGMTE------IG----MALSNPLTGPRIPGSVGVPLPGVDVRIVmnnKINTsivEGNH*NTWV 423
Cdd:cd05910 217 ARLRKMLSDEaeILTPYGATEalpvssIGsrelLATTTAATSGGAGTCVGRPIPGVRVRII---EIDD---EPIAEWDDT 290
|
250 260 270 280
....*....|....*....|....*....|....*....|....*.
gi 928020699 424 RPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDDG----WFKTG 465
Cdd:cd05910 291 LELPRGEIGEITVTGPTVTPTYVNRPVATALAKIDDNsegfWHRMG 336
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
551-664 |
4.94e-11 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 65.78 E-value: 4.94e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 551 GD-TAIYKDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRkgKSLTLPELQ 629
Cdd:PRK10946 414 GDlVSIDPDGYITVVGREK-DQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVK--EPLKAVQLR 490
|
90 100 110
....*....|....*....|....*....|....*.
gi 928020699 630 TWAREH-MGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK10946 491 RFLREQgIAEFKLPDRVECVDSLPLTAVGKVDKKQL 526
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
108-465 |
6.35e-11 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 65.96 E-value: 6.35e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 108 GKRISFLCANDSSYVVAQWAVWMSGGTAVPLY-----RKHPPSELEYIISDSQSSLVVAGHPYEEILQPLAQKLGLPCLP 182
Cdd:PRK05691 64 GDRAVLLFPSGPDYVAAFFGCLYAGVIAVPAYppesaRRHHQERLLSIIADAEPRLLLTVADLRDSLLQMEELAAANAPE 143
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 183 LPPTNNLDALDETDTREKEITitdwTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKN--DIILHTLPLH 260
Cdd:PRK05691 144 LLCVDTLDPALAEAWQEPALQ----PDDIAFLQYTSGSTALPKGVQVSHGNLVANEQLIRHGFGIDLNpdDVIVSWLPLY 219
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 261 HLHGIVNKLLCPLWVGATCVmlpdfqaqkvwemLLSSkaptiNVFMAVPTIYSKLIQFYDQHYQ-QPllkEFVKAVCKER 339
Cdd:PRK05691 220 HDMGLIGGLLQPIFSGVPCV-------------LMSP-----AYFLERPLRWLEAISEYGGTISgGP---DFAYRLCSER 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 340 I-------------RLMVSGSAALPLPTLQRWKE------ITGHTLLERYGMTEIGMALSNPLTGPRIP----------- 389
Cdd:PRK05691 279 VsesalerldlsrwRVAYSGSEPIRQDSLERFAEkfaacgFDPDSFFASYGLAEATLFVSGGRRGQGIPaleldaealar 358
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 390 -------GSV----GVPLPGVDVRIVmnnKINTSIVEGnh*ntwvrpglEGKEGELLVRGQSVFKEYWNKPQETRESFTD 458
Cdd:PRK05691 359 nraepgtGSVlmscGRSQPGHAVLIV---DPQSLEVLG-----------DNRVGEIWASGPSIAHGYWRNPEASAKTFVE 424
|
410
....*....|
gi 928020699 459 -DG--WFKTG 465
Cdd:PRK05691 425 hDGrtWLRTG 434
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
122-664 |
7.59e-11 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 65.96 E-value: 7.59e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAGHPYEEILQPLAQklglPCLPLPPTNNLDALDETDTreKE 201
Cdd:PRK05691 2252 VVGLLAILKAGGAYVPLDPEYPLERLHYMIEDSGIGLLLSDRALFEALGELPA----GVARWCLEDDAAALAAYSD--AP 2325
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 202 ITITDWTDRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDiILHTLPLHHLHGIVNKLLCPLWVGATCVM 281
Cdd:PRK05691 2326 LPFLSLPQHQAYLIYTSGSTGKPKGVVVSHGEIAMHCQAVIERFGMRADD-CELHFYSINFDAASERLLVPLLCGARVVL 2404
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 282 lpdfQAQKVW---EMLLSSKAPTINVFMAVPTIYSKLIQFYDQHYQQ-PllkefvkavckerIRLMVSGSAALPLPTLQR 357
Cdd:PRK05691 2405 ----RAQGQWgaeEICQLIREQQVSILGFTPSYGSQLAQWLAGQGEQlP-------------VRMCITGGEALTGEHLQR 2467
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 358 WKEITGHTLL-ERYGMTE-IGMALSNpLTGPRIP-GSVGVPLPgvdvRIVMNNKinTSIVEGNh*ntwVRPGLEGKEGEL 434
Cdd:PRK05691 2468 IRQAFAPQLFfNAYGPTEtVVMPLAC-LAPEQLEeGAASVPIG----RVVGARV--AYILDAD-----LALVPQGATGEL 2535
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 435 LVRGQSVFKEYWNKPQETRESFTDDGwfktgtepslsspafvlthFSAADVRtlidmlpgkgvnsaycsctirrcrgtlp 514
Cdd:PRK05691 2536 YVGGAGLAQGYHDRPGLTAERFVADP-------------------FAADGGR---------------------------- 2568
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 515 reercqtsfspMLQTGltptmt*ltSLQRfahihpLGDtaiykDGVYWIMGRTSVDIlKSGGFKISALEVECHLLAHPDI 594
Cdd:PRK05691 2569 -----------LYRTG---------DLVR------LRA-----DGLVEYVGRIDHQV-KIRGFRIELGEIESRLLEHPAV 2616
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 928020699 595 ADVAVIgAPDATWGQK-----VTAVVQLRKGKSLTLPE-LQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK05691 2617 REAVVL-ALDTPSGKQlagylVSAVAGQDDEAQAALREaLKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRAL 2691
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
80-465 |
9.80e-11 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 64.84 E-value: 9.80e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 80 SYKQLYCSSLglasRISSALGSDfGGLDGKRISFLCANdssyvVAQWAVWMSGGTA-----VPLYRKHPPSELEYIISDS 154
Cdd:PLN02430 78 TYKEVYEEVL----QIGSALRAS-GAEPGSRVGIYGSN-----CPQWIVAMEACAAhslicVPLYDTLGPGAVDYIVDHA 147
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 155 QSSLV-VAGHPYEEILQPLAQKLGLPCLPLPPTNnldALDETDTREKEITIT--DWTDRPAM------------------ 213
Cdd:PLN02430 148 EIDFVfVQDKKIKELLEPDCKSAKRLKAIVSFTS---VTEEESDKASQIGVKtySWIDFLHMgkenpsetnppkpldict 224
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 214 IIYTSGTTGRPKGVLHTHssiQAMTQCLVSAwawsknDIILHTLPLHHLHGIVNKLLCPL-------------------- 273
Cdd:PLN02430 225 IMYTSGTSGDPKGVVLTH---EAVATFVRGV------DLFMEQFEDKMTHDDVYLSFLPLahildrmieeyffrkgasvg 295
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 274 -WVGATCVMLPDFQAQKvwemllsskaPTInvFMAVPTIYSKL---IQ-------------F--------------YDQH 322
Cdd:PLN02430 296 yYHGDLNALRDDLMELK----------PTL--LAGVPRVFERIhegIQkalqelnprrrliFnalykyklawmnrgYSHK 363
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 323 YQQPL--LKEF--VKAVCKERIRLMVSGSAALPLPTLQRWKEITGHTLLERYGMTEI--GMALSNPLTGPRIpGSVGVPL 396
Cdd:PLN02430 364 KASPMadFLAFrkVKAKLGGRLRLLISGGAPLSTEIEEFLRVTSCAFVVQGYGLTETlgPTTLGFPDEMCML-GTVGAPA 442
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 928020699 397 PGVDVRIvmnnkinTSIVEGNH*ntwvRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTdDGWFKTG 465
Cdd:PLN02430 443 VYNELRL-------EEVPEMGY-----DPLGEPPRGEICVRGKCLFSGYYKNPELTEEVMK-DGWFHTG 498
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
209-465 |
2.14e-10 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 63.66 E-value: 2.14e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGI-----------VNKLLCP----- 272
Cdd:cd05908 106 DELAFIQFSSGSTGDPKGVMLTHENLVHNMFAILNSTEWKTKDRILSWMPLTHDMGLiafhlapliagMNQYLMPtrlfi 185
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 273 ----LWV------GATCVMLPDFQAQKVWEMLLSSKA-----PTINVFM--AVPTIYSkliqfydqhyqqpLLKEFVKAV 335
Cdd:cd05908 186 rrpiLWLkkasehKATIVSSPNFGYKYFLKTLKPEKAndwdlSSIRMILngAEPIDYE-------------LCHEFLDHM 252
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 336 CKERIR----LMVSG----SAALPLPTLQ-RWKEITGHTLLERYGMTEIGMALSNPlTGPRIPgSVGVPLPGVDVRIVMN 406
Cdd:cd05908 253 SKYGLKrnaiLPVYGlaeaSVGASLPKAQsPFKTITLGRRHVTHGEPEPEVDKKDS-ECLTFV-EVGKPIDETDIRICDE 330
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*....
gi 928020699 407 NkintsivegNH*ntwVRPglEGKEGELLVRGQSVFKEYWNKPQETRESFTDDGWFKTG 465
Cdd:cd05908 331 D---------NK----ILP--DGYIGHIQIRGKNVTPGYYNNPEATAKVFTDDGWLKTG 374
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
551-664 |
2.20e-10 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 63.13 E-value: 2.20e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 551 GDTAIY-KDGVYW-------IMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGks 622
Cdd:PRK08308 289 GDKEIFtKDLGYKsergtlhFMGRMD-DVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVISHEE-- 365
|
90 100 110 120
....*....|....*....|....*....|....*....|..
gi 928020699 623 LTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK08308 366 IDPVQLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
216-664 |
4.34e-10 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 62.55 E-value: 4.34e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 216 YTSGTTGRPKGVLHTHSSIQAMtqCLVSAWAWSKNDiilhtlplhhlhGIVNKLLCPL---------WVGA----TCVML 282
Cdd:PLN02479 202 YTSGTTASPKGVVLHHRGAYLM--ALSNALIWGMNE------------GAVYLWTLPMfhcngwcftWTLAalcgTNICL 267
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 283 PDFQAQKVWEMLLSSKaptINVFMAVPTIYSKLIQFYDQHYQQPLlkefvkavcKERIRLMVSGSAalPLPTLQRWKEIT 362
Cdd:PLN02479 268 RQVTAKAIYSAIANYG---VTHFCAAPVVLNTIVNAPKSETILPL---------PRVVHVMTAGAA--PPPSVLFAMSEK 333
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 363 GHTLLERYGMTEIgMALSN------------PLTGPRIPGSVGVPLPGVDVRIVMNNKINTSIvegnh*ntwvrPGLEGK 430
Cdd:PLN02479 334 GFRVTHTYGLSET-YGPSTvcawkpewdslpPEEQARLNARQGVRYIGLEGLDVVDTKTMKPV-----------PADGKT 401
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 431 EGELLVRGQSVFKEYWNKPQETRESFTDdGWFKTGtepslsspafvlthfsaadvrtliDMlpgkGVNsaycsctirrcr 510
Cdd:PLN02479 402 MGEIVMRGNMVMKGYLKNPKANEEAFAN-GWFHSG------------------------DL----GVK------------ 440
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 511 gtlpreercqtsfspmlqtgltptmt*ltslqrfahiHPlgdtaiykDGVYWIMGRtSVDILKSGGFKISALEVECHLLA 590
Cdd:PLN02479 441 -------------------------------------HP--------DGYIEIKDR-SKDIIISGGENISSLEVENVVYT 474
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 928020699 591 HPDIADVAVIGAPDATWGQKVTAVVQLRKG-----KSLTLPELQTWAREHMGPYKIPTGLLLvEEMPRNQMGKINKKDL 664
Cdd:PLN02479 475 HPAVLEASVVARPDERWGESPCAFVTLKPGvdksdEAALAEDIMKFCRERLPAYWVPKSVVF-GPLPKTATGKIQKHVL 552
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
209-465 |
5.03e-10 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 63.06 E-value: 5.03e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPD-FQA 287
Cdd:PRK06814 793 DDPAVILFTSGSEGTPKGVVLSHRNLLANRAQVAARIDFSPEDKVFNALPVFHSFGLTGGLVLPLLSGVKVFLYPSpLHY 872
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 288 QKVWEMLLSSKApTI----NVFMavpTIYSKLIQFYDqhyqqpllkeFvkavckERIRLMVSGSAALPLPTLQRWKEITG 363
Cdd:PRK06814 873 RIIPELIYDTNA-TIlfgtDTFL---NGYARYAHPYD----------F------RSLRYVFAGAEKVKEETRQTWMEKFG 932
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 364 HTLLERYGMTEIG--MALSNPLTGPriPGSVGVPLPGVDVRIVMnnkintsiVEGNH*ntwvrpglEGkeGELLVRGQSV 441
Cdd:PRK06814 933 IRILEGYGVTETApvIALNTPMHNK--AGTVGRLLPGIEYRLEP--------VPGID---------EG--GRLFVRGPNV 991
|
250 260
....*....|....*....|....*.
gi 928020699 442 FKEYW--NKPQETREsfTDDGWFKTG 465
Cdd:PRK06814 992 MLGYLraENPGVLEP--PADGWYDTG 1015
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
212-465 |
6.11e-10 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 62.14 E-value: 6.11e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 212 AMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPD-FQAQKV 290
Cdd:PRK06334 186 AVILFTSGTEKLPKGVPLTHANLLANQRACLKFFSPKEDDVMMSFLPPFHAYGFNSCTLFPLLSGVPVVFAYNpLYPKKI 265
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 291 WEMLLSSKAptinvfmavpTIYSKLIQFYDQhyqqpLLKEFVKA-VCKERIRLMVSGSAALPLPTLQRWKEITGH-TLLE 368
Cdd:PRK06334 266 VEMIDEAKV----------TFLGSTPVFFDY-----ILKTAKKQeSCLPSLRFVVIGGDAFKDSLYQEALKTFPHiQLRQ 330
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 369 RYGMTEIGMALS-NPLTGPRIPGSVGVPLPGVDVRIVmnnkintsivegnH*NTWVrPGLEGKEGELLVRGQSVFKEYW- 446
Cdd:PRK06334 331 GYGTTECSPVITiNTVNSPKHESCVGMPIRGMDVLIV-------------SEETKV-PVSSGETGLVLTRGTSLFSGYLg 396
|
250
....*....|....*....
gi 928020699 447 NKPQETRESFTDDGWFKTG 465
Cdd:PRK06334 397 EDFGQGFVELGGETWYVTG 415
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
122-664 |
9.42e-10 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 61.98 E-value: 9.42e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVVAghpyEEILQPLAQKLGLPCLPLPPTNNLDALDETDTREKe 201
Cdd:PRK10252 522 TLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLIT----TADQLPRFADVPDLTSLCYNAPLAPQGAAPLQLSQ- 596
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 202 ititdwTDRPAMIIYTSGTTGRPKGVLHTHSSiqamtqclvsawawskndiilhtlplhhlhgIVNKLlcpLW------V 275
Cdd:PRK10252 597 ------PHHTAYIIFTSGSTGRPKGVMVGQTA-------------------------------IVNRL---LWmqnhypL 636
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 276 GATCVMLpdfqaQK--------VWEMLLsskaPTI---NVFMAVPTIY---SKLIQFYDQ------HYQQPLLKEFVKAV 335
Cdd:PRK10252 637 TADDVVL-----QKtpcsfdvsVWEFFW----PFIagaKLVMAEPEAHrdpLAMQQFFAEygvtttHFVPSMLAAFVASL 707
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 336 CKERI--------RLMVSGSaALPLPTLQRWKEITGHTLLERYGMTEIGMALSN-PLTGPRIPGSVGVPLP-GVDVrivm 405
Cdd:PRK10252 708 TPEGArqscaslrQVFCSGE-ALPADLCREWQQLTGAPLHNLYGPTEAAVDVSWyPAFGEELAAVRGSSVPiGYPV---- 782
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 406 nnkINTS--IVEGNh*ntwVRPGLEGKEGELLVRGQSVFKEYWNKPQETRESFTDDgwfktgtepslssPAfvlthfsaa 483
Cdd:PRK10252 783 ---WNTGlrILDAR-----MRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIAD-------------PF--------- 832
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 484 dvrtlidmLPGkgvnsaycsctirrcrgtlpreERcqtsfspMLQTGltptmt*ltSLQRFahihpLGDTAIykdgVYwi 563
Cdd:PRK10252 833 --------APG----------------------ER-------MYRTG---------DVARW-----LDDGAV----EY-- 855
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 564 MGRtSVDILKSGGFKISALEVECHLLAHPDIADVA----VIGAPDATWG---QKVTAVVQlRKGKSLTLPELQTWAREHM 636
Cdd:PRK10252 856 LGR-SDDQLKIRGQRIELGEIDRAMQALPDVEQAVthacVINQAAATGGdarQLVGYLVS-QSGLPLDTSALQAQLRERL 933
|
570 580
....*....|....*....|....*...
gi 928020699 637 GPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK10252 934 PPHMVPVVLLQLDQLPLSANGKLDRKAL 961
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
122-664 |
5.76e-09 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 58.95 E-value: 5.76e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 122 VVAQWAVWMSGGTAVPLYRKHPPSELEYIISDSQSSLVvaghpyeeilqplaqklglpclplpptnnldaldetdtreke 201
Cdd:cd17648 52 IIAILAVWKAGAAYVPIDPSYPDERIQFILEDTGARVV------------------------------------------ 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 202 itITDWTDRpAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKN-DIILHTLPLHHLHGIVNKLLCPLWVGATCV 280
Cdd:cd17648 90 --ITNSTDL-AYAIYTSGTTGKPKGVLVEHGSVVNLRTSLSERYFGRDNgDEAVLFFSNYVFDFFVEQMTLALLNGQKLV 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 281 MLPDfqaqkvwEMLLSSKA--PTIN-----VFMAVPTiyskLIQFYDqHYQQPLLKefvkavckeriRLMVSGSaALPLP 353
Cdd:cd17648 167 VPPD-------EMRFDPDRfyAYINrekvtYLSGTPS----VLQQYD-LARLPHLK-----------RVDAAGE-EFTAP 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 354 TLQRWKEITGHTLLERYGMTEIGM-ALSNP-LTGPRIPGSVGVPLPGVDVRIVmnnkintsivegnh*NTWVRPGLEGKE 431
Cdd:cd17648 223 VFEKLRSRFAGLIINAYGPTETTVtNHKRFfPGDQRFDKSLGRPVRNTKCYVL---------------NDAMKRVPVGAV 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 432 GELLVRGQSVFKEYWNKPQETRESFtddgwfktgtepsLSSPafvlthFSAADvrtlidmlpgkgvnsaycsctiRRCRG 511
Cdd:cd17648 288 GELYLGGDGVARGYLNRPELTAERF-------------LPNP------FQTEQ----------------------ERARG 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 512 TLPReercqtsfspMLQTGltptmt*ltSLQRFAHihplgdtaiykDGVYWIMGRTSVDIlKSGGFKISALEVECHLLAH 591
Cdd:cd17648 327 RNAR----------LYKTG---------DLVRWLP-----------SGELEYLGRNDFQV-KIRGQRIEPGEVEAALASY 375
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 928020699 592 PDIADVAVIGAPDATWGQK--VTAVVQLRKGKSLTLPE--LQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:cd17648 376 PGVRECAVVAKEDASQAQSriQKYLVGYYLPEPGHVPEsdLLSFLRAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
551-664 |
7.11e-09 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 58.88 E-value: 7.11e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 551 GDTAiYKDGVYWI--MGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPEL 628
Cdd:PRK13388 385 GDLA-YRDADGWIyfAGRTA-DWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGATFDPDAF 462
|
90 100 110
....*....|....*....|....*....|....*...
gi 928020699 629 QTW--AREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK13388 463 AAFlaAQPDLGTKAWPRYVRIAADLPSTATNKVLKREL 500
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
568-666 |
4.71e-08 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 55.90 E-value: 4.71e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 568 SVDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTlPELQTWAREHMGPYK-IPTGLL 646
Cdd:cd05915 411 LKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVVVPRGEKPTP-EELNEHLLKAGFAKWqLPDAYV 489
|
90 100
....*....|....*....|
gi 928020699 647 LVEEMPRNQMGKINKKDLLK 666
Cdd:cd05915 490 FAEEIPRTSAGKFLKRALRE 509
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
552-627 |
8.08e-08 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 55.34 E-value: 8.08e-08
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 928020699 552 DTAIY-KDGVYWIMGRTSvDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKSLTLPE 627
Cdd:PRK10524 479 DWGIRdADGYYFILGRTD-DVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPKDSDSLADRE 554
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
209-664 |
1.29e-07 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 54.75 E-value: 1.29e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVlhthssIQAMTQCLVSAWAWSK------ND----IILHTLPLHHLHGIVNKLLCplwvGAT 278
Cdd:cd05937 87 DDPAILIYTSGTTGLPKAA------AISWRRTLVTSNLLSHdlnlknGDrtytCMPLYHGTAAFLGACNCLMS----GGT 156
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 279 CVMLPDFQAQKVWEMLLSSKApTINVF--------MAVPtiysklIQFYDQhyqqpllkefvkavcKERIRlMVSGSAAL 350
Cdd:cd05937 157 LALSRKFSASQFWKDVRDSGA-TIIQYvgelcrylLSTP------PSPYDR---------------DHKVR-VAWGNGLR 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 351 PlPTLQRWKEITG-HTLLERYGMTEIGMALSNPLTGPRIPGSVGvpLPGVDVRIVMNNKINTSIVEGNH*NTWVRP--GL 427
Cdd:cd05937 214 P-DIWERFRERFNvPEIGEFYAATEGVFALTNHNVGDFGAGAIG--HHGLIRRWKFENQVVLVKMDPETDDPIRDPktGF 290
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 428 -----EGKEGELLVR----GQSVFKEYWNKPQETRESF-TD-----DGWFKTGtepslsspafvlthfsaadvrtliDMl 492
Cdd:cd05937 291 cvrapVGEPGEMLGRvpfkNREAFQGYLHNEDATESKLvRDvfrkgDIYFRTG------------------------DL- 345
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 493 pgkgvnsaycsctirrcrgtLPREERCQTSFspmlqtgltptmt*ltsLQRfahihpLGDTAIYKdgvywimgrtsvdil 572
Cdd:cd05937 346 --------------------LRQDADGRWYF-----------------LDR------LGDTFRWK--------------- 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 573 ksgGFKISALEVECHLLAHPDIADVAVIGA--PDATwGQKVTAVVQLRKGKS----LTLPELQTWAREHMGPYKIPTGLL 646
Cdd:cd05937 368 ---SENVSTTEVADVLGAHPDIAEANVYGVkvPGHD-GRAGCAAITLEESSAvpteFTKSLLASLARKNLPSYAVPLFLR 443
|
490
....*....|....*...
gi 928020699 647 LVEEMPRNQMGKINKKDL 664
Cdd:cd05937 444 LTEEVATTDNHKQQKGVL 461
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
123-465 |
1.75e-07 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 54.23 E-value: 1.75e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 123 VAQwAVWMSGGTAVPLYRKHPPSELEYIISDS-------QSSLVVAGHPYEEILQPLAQKLGLPCLPLpptnnlDALDET 195
Cdd:PRK07768 70 TAQ-GLWMRGASLTMLHQPTPRTDLAVWAEDTlrvigmiGAKAVVVGEPFLAAAPVLEEKGIRVLTVA------DLLAAD 142
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 196 DTREKEITitdwTDRPAMIIYTSGTTGRPKGVLHTH----SSIQAMTQclvSAWAWSKNDIILHTLPLHHLHGIVNKLLC 271
Cdd:PRK07768 143 PIDPVETG----EDDLALMQLTSGSTGSPKAVQITHgnlyANAEAMFV---AAEFDVETDVMVSWLPLFHDMGMVGFLTV 215
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 272 PLWVGATCVML-P-DFQAQK-VWEMLLSSKAPTINvfmAVP----TIYSKLIQFYDQHYQQPLlkefvkavckERIRLMV 344
Cdd:PRK07768 216 PMYFGAELVKVtPmDFLRDPlLWAELISKYRGTMT---AAPnfayALLARRLRRQAKPGAFDL----------SSLRFAL 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 345 SGSAALPLPTLQRWKEITG------HTLLERYGMTEIGMALSNPLTG--------------------PRIPG------SV 392
Cdd:PRK07768 283 NGAEPIDPADVEDLLDAGArfglrpEAILPAYGMAEATLAVSFSPCGaglvvdevdadllaalrravPATKGntrrlaTL 362
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 928020699 393 GVPLPGVDVRIVMNNkintsiveGNh*ntwVRPglEGKEGELLVRGQSVFKEYwnkpqETRESFT----DDGWFKTG 465
Cdd:PRK07768 363 GPPLPGLEVRVVDED--------GQ-----VLP--PRGVGVIELRGESVTPGY-----LTMDGFIpaqdADGWLDTG 419
|
|
| PTZ00342 |
PTZ00342 |
acyl-CoA synthetase; Provisional |
432-465 |
9.21e-07 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240370 [Multi-domain] Cd Length: 746 Bit Score: 52.03 E-value: 9.21e-07
10 20 30
....*....|....*....|....*....|....
gi 928020699 432 GELLVRGQSVFKEYWNKPQETRESFTDDGWFKTG 465
Cdd:PTZ00342 542 GELLIKSDSIFSGYFLEKEQTKNAFTEDGYFKTG 575
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
209-664 |
2.33e-06 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 51.32 E-value: 2.33e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWvGATCVMLPDFQAQ 288
Cdd:PRK05691 3869 DNLAYVIYTSGSTGLPKGVMVEQRGMLNNQLSKVPYLALSEADVIAQTASQSFDISVWQFLAAPLF-GARVEIVPNAIAH 3947
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 289 KVWEMLLSSKAPTINVFMAVPTiyskLIQFYDQHYQQPLlkefvkavckERIRLMVSGSAALPLPTLQRWkeitghtlLE 368
Cdd:PRK05691 3948 DPQGLLAHVQAQGITVLESVPS----LIQGMLAEDRQAL----------DGLRWMLPTGEAMPPELARQW--------LQ 4005
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 369 RYgmTEIGmaLSNPLtGPrIPGSVGVPLPGVDVRIVMNNKINTSIVEGNH*NTWVRPGLE----GKEGELLVRGQSVFKE 444
Cdd:PRK05691 4006 RY--PQIG--LVNAY-GP-AECSDDVAFFRVDLASTRGSYLPIGSPTDNNRLYLLDEALElvplGAVGELCVAGTGVGRG 4079
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 445 YWNKPQETresftddgwfktgtepslsSPAFVLTHFSAadvrtlidmlPGKgvnsaycsctiRRCR-GTLPReercqtsf 523
Cdd:PRK05691 4080 YVGDPLRT-------------------ALAFVPHPFGA----------PGE-----------RLYRtGDLAR-------- 4111
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 524 spmlqtgltptmt*ltslQRfahihplgdtaiyKDGVYWIMGRTSVDIlKSGGFKISALEVECHLLAHPDIADVAViGAP 603
Cdd:PRK05691 4112 ------------------RR-------------SDGVLEYVGRIDHQV-KIRGYRIELGEIEARLHEQAEVREAAV-AVQ 4158
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 928020699 604 DATWGQKVTAVVQLRKG---KSLTLPELQTWAREHMGPYKIPTGLLLVEEMPRNQMGKINKKDL 664
Cdd:PRK05691 4159 EGVNGKHLVGYLVPHQTvlaQGALLERIKQRLRAELPDYMVPLHWLWLDRLPLNANGKLDRKAL 4222
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
201-292 |
8.96e-06 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 48.83 E-value: 8.96e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 201 EITITDwtdrPAMIIYTSGTTGRPKGVLHTHSSIQAMTQ----CLVSAwawskNDIILHTLPLHHLHGIVNKLLCPLWVG 276
Cdd:cd05938 140 HVTIKS----PALYIYTSGTTGLPKAARISHLRVLQCSGflslCGVTA-----DDVIYITLPLYHSSGFLLGIGGCIELG 210
|
90
....*....|....*.
gi 928020699 277 ATCVMLPDFQAQKVWE 292
Cdd:cd05938 211 ATCVLKPKFSASQFWD 226
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
212-465 |
4.15e-05 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 46.65 E-value: 4.15e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 212 AMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAW-AWSKNDIILHTLPLHHLHGIVNKLLCpLWVGA-----TCVMLPDF 285
Cdd:PLN02387 253 AVIMYTSGSTGLPKGVMMTHGNIVATVAGVMTVVpKLGKNDVYLAYLPLAHILELAAESVM-AAVGAaigygSPLTLTDT 331
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 286 --QAQKVWEMLLSSKAPTInvFMAVPTIYSKL---------------IQFYDQHYQ----------------QPLLKEF- 331
Cdd:PLN02387 332 snKIKKGTKGDASALKPTL--MTAVPAILDRVrdgvrkkvdakgglaKKLFDIAYKrrlaaiegswfgawglEKLLWDAl 409
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 332 ----VKAVCKERIRLMVSGSAALPLPTlQRWKEIT-GHTLLERYGMTEI--GMALSNPlTGPRIpGSVGVPLPGVDVRIV 404
Cdd:PLN02387 410 vfkkIRAVLGGRIRFMLSGGAPLSGDT-QRFINIClGAPIGQGYGLTETcaGATFSEW-DDTSV-GRVGPPLPCCYVKLV 486
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 928020699 405 mnnkintSIVEGNH*NTwVRPgleGKEGELLVRGQSVFKEYWNKPQETRESFTDDG----WFKTG 465
Cdd:PLN02387 487 -------SWEEGGYLIS-DKP---MPRGEIVIGGPSVTLGYFKNQEKTDEVYKVDErgmrWFYTG 540
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
209-464 |
7.42e-05 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 45.63 E-value: 7.42e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 209 DRPAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDiilhtlplhhlhgivNKLLC-PL-----------W-- 274
Cdd:PRK09029 135 QRLATMTLTSGSTGLPKAAVHTAQAHLASAEGVLSLMPFTAQD---------------SWLLSlPLfhvsgqgivwrWly 199
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 275 VGATCVmLPDfqAQKVWEMLlsskaptINVFMA--VPTIYSKLIqfydQHYQQPLLkefVKAVckerirLMvsGSAALPl 352
Cdd:PRK09029 200 AGATLV-VRD--KQPLEQAL-------AGCTHAslVPTQLWRLL----DNRSEPLS---LKAV------LL--GGAAIP- 253
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 353 ptlqrwkeitgHTLLER-----------YGMTEigMAlsNPLTGPRIPGS--VGVPLPGVDVRIVmnnkintsivegnh* 419
Cdd:PRK09029 254 -----------VELTEQaeqqgircwcgYGLTE--MA--STVCAKRADGLagVGSPLPGREVKLV--------------- 303
|
250 260 270 280
....*....|....*....|....*....|....*....|....*.
gi 928020699 420 ntwvrpglegkEGELLVRGQSVFKEYWNKPQETreSFTD-DGWFKT 464
Cdd:PRK09029 304 -----------DGEIWLRGASLALGYWRQGQLV--PLVNdEGWFAT 336
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
212-417 |
1.24e-04 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 45.16 E-value: 1.24e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 212 AMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCpLWVGATCVMLPDfqAQKVW 291
Cdd:cd17654 121 AYVIHTSGTTGTPKIVAVPHKCILPNIQHFRSLFNITSEDILFLTSPLTFDPSVVEIFLS-LSSGATLLIVPT--SVKVL 197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 292 EMLLSS---KAPTINVFMAVPTIyskLIQFYDQhyqqpLLKEFVKAVCKErIRLMVSGSAALPLPTLQR-W-KEITGHTL 366
Cdd:cd17654 198 PSKLADilfKRHRITVLQATPTL---FRRFGSQ-----SIKSTVLSATSS-LRVLALGGEPFPSLVILSsWrGKGNRTRI 268
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|..
gi 928020699 367 LERYGMTEIGM-ALSNPLTGPRIPGSVGVPLPGVDVRIVmnnKINTSIVEGN 417
Cdd:cd17654 269 FNIYGITEVSCwALAYKVPEEDSPVQLGSPLLGTVIEVR---DQNGSEGTGQ 317
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
186-291 |
9.22e-04 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 42.41 E-value: 9.22e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 186 TNNLDALDETDTRE-KEITITDWTDRpAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTL-PLHHLH 263
Cdd:cd05939 81 FNLLDPLLTQSSTEpPSQDDVNFRDK-LFYIYTSGTTGLPKAAVIVHSRYYRIAAGAYYAFGMRPEDVVYDCLpLYHSAG 159
|
90 100
....*....|....*....|....*...
gi 928020699 264 GIVNKLLCPLWvGATCVMLPDFQAQKVW 291
Cdd:cd05939 160 GIMGVGQALLH-GSTVVIRKKFSASNFW 186
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
81-465 |
2.63e-03 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 40.76 E-value: 2.63e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 81 YKQLYCSSLGLASRISSAlgsdfgGLD-GKRISFLCANDSSYVVAQWAVWMSGGTAVPL-----------YRKHppseLE 148
Cdd:PRK09192 52 YQTLRARAEAGARRLLAL------GLKpGDRVALIAETDGDFVEAFFACQYAGLVPVPLplpmgfggresYIAQ----LR 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 149 YIISDSQSSLVVAGHPYEEILQPLAQKLGLPCLPLPPtnNLDALDETDTREKEITITDwtdrPAMIIYTSGTTGRPKGVL 228
Cdd:PRK09192 122 GMLASAQPAAIITPDELLPWVNEATHGNPLLHVLSHA--WFKALPEADVALPRPTPDD----IAYLQYSSGSTRFPRGVI 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 229 HTHSSIQAMTQCLVS------------AWAWSKNDIilhtlplhhlhGIVNKLLCPLWVGATCVMLP--DFqAQK--VWE 292
Cdd:PRK09192 196 ITHRALMANLRAISHdglkvrpgdrcvSWLPFYHDM-----------GLVGFLLTPVATQLSVDYLPtrDF-ARRplQWL 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 293 MLLSSKAPTINvfmavptiyskliqfydqhYQQPllkeFVKAVCKERIRlmvsgSAALPLPTLQRWK-------EITGHT 365
Cdd:PRK09192 264 DLISRNRGTIS-------------------YSPP----FGYELCARRVN-----SKDLAELDLSCWRvagigadMIRPDV 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 366 L-----------------LERYGMTEIGMALSNPltgpriPGSVGVPLPGVDVRIVMNNKIN-TSIVEGNH*NTWVR--- 424
Cdd:PRK09192 316 LhqfaeafapagfddkafMPSYGLAEATLAVSFS------PLGSGIVVEEVDRDRLEYQGKAvAPGAETRRVRTFVNcgk 389
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*.
gi 928020699 425 --PGLE-------GKE------GELLVRGQSVFKEYWNKpQETRESFTDDGWFKTG 465
Cdd:PRK09192 390 alPGHEieirneaGMPlpervvGHICVRGPSLMSGYFRD-EESQDVLAADGWLDTG 444
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
211-291 |
3.14e-03 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 40.41 E-value: 3.14e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 211 PAMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDIILHTLPLHHLHGIVNKLLCPLWVGATCVMLPDFQAQKV 290
Cdd:cd05940 83 AALYIYTSGTTGLPKAAIISHRRAWRGGAFFAGSGGALPSDVLYTCLPLYHSTALIVGWSACLASGATLVIRKKFSASNF 162
|
.
gi 928020699 291 W 291
Cdd:cd05940 163 W 163
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
556-659 |
3.78e-03 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 40.50 E-value: 3.78e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 556 YKD--GVYWIMGRtSVDILKSGGFKISALEVECHLLAHPDIADVAVIGAPDATWGQKVTAVVQLRKGKS---LTLPELQT 630
Cdd:PTZ00237 501 FKDenGYYTIVSR-SDDQIKISGNKVQLNTIETSILKHPLVLECCSIGIYDPDCYNVPIGLLVLKQDQSnqsIDLNKLKN 579
|
90 100 110
....*....|....*....|....*....|...
gi 928020699 631 WARE----HMGPYKIPTGLLLVEEMPRNQMGKI 659
Cdd:PTZ00237 580 EINNiitqDIESLAVLRKIIIVNQLPKTKTGKI 612
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
211-227 |
4.21e-03 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 40.32 E-value: 4.21e-03
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
147-253 |
4.25e-03 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 40.24 E-value: 4.25e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 928020699 147 LEYIISDSQSSLVVAGH----PYEEILQPLAQKLGLPCLPLPPTNNLDALDETDTR-----------EKEITITDwtdrP 211
Cdd:PRK08279 126 LAHSLNLVDAKHLIVGEelveAFEEARADLARPPRLWVAGGDTLDDPEGYEDLAAAaagapttnpasRSGVTAKD----T 201
|
90 100 110 120
....*....|....*....|....*....|....*....|..
gi 928020699 212 AMIIYTSGTTGRPKGVLHTHSSIQAMTQCLVSAWAWSKNDII 253
Cdd:PRK08279 202 AFYIYTSGTTGLPKAAVMSHMRWLKAMGGFGGLLRLTPDDVL 243
|
|
| |
