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Conserved domains on  [gi|767993636|ref|XP_011522658|]
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AP-4 complex accessory subunit tepsin isoform X3 [Homo sapiens]

Protein Classification

ENTH domain-containing protein( domain architecture ID 10132404)

ENTH (Epsin N-Terminal Homology) domain-containing protein may be involved in clathrin-mediated endocytosis; similar to Homo sapiens AP-4 complex accessory subunit Tepsin that associates with the adapter-like complex 4 (AP-4) and may therefore play a role in vesicular trafficking of proteins at the trans-Golgi network

CATH:  1.25.40.90
Gene Ontology:  GO:0030276|GO:1901981|GO:0006897|GO:0072659|GO:0005543
PubMed:  27466364|14657269|12742163|22748146|11911874|29774507
SCOP:  4001355

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ENTH_like_Tepsin cd03572
Epsin N-Terminal Homology (ENTH)-like domain of AP-4 complex accessory subunit Tepsin and ...
 227-346 2.07e-52

Epsin N-Terminal Homology (ENTH)-like domain of AP-4 complex accessory subunit Tepsin and similar domains; This family is composed of proteins containing an ENTH-like domain including vertebrate AP-4 complex accessory subunit Tepsin and Arabidopsis thaliana VHS domain-containing protein At3g16270. Tepsin is also called ENTH Domain-containing protein 2 (ENTHD2), Epsin for AP-4, or Tetra-epsin. It associates with the adapter-like complex 4 (AP-4), a heterotetramer composed of two large adaptins (epsilon and beta), a medium adaptin (mu) and a small adaptin (sigma), which forms a non-clathrin coat on vesicles departing the Trans-Golgi Network. The Epsin N-Terminal Homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. ENTH domain is highly similar to the N-terminal region of the AP180 N-Terminal Homology (ANTH_N) domain. ENTH and ANTH_N domains are structurally similar to the VHS domain and are composed of a superhelix of eight alpha helices. ENTH domains bind both, inositol phospholipids with preference for PtdIns(4,5)P2, and proteins, and contribute to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. ENTH and ANTH (E/ANTH)-containing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.


:

Pssm-ID: 340773  Cd Length: 119  Bit Score: 177.36  E-value: 2.07e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636 227 LPILLKGTSDDDVPCPGYLFEEIAtaEISHESPGSSQCLLEYLLSRLHSSSGHGKLKVLKILLYLCSHGSSFFLLILKRN 306
Cdd:cd03572    2 RPLLDKATSDDDEPTPGYLLEEIA--KLTRSSPGSCQELLDYLLKRLKKSSPHVKLKALRIIKHLCQKGSPEFRRELQRN 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 767993636 307 SAFIQEAAAFAGPPDPLHGNSLYQKVRAAAQDLGSTLFSD 346
Cdd:cd03572   80 SAAIRECTSYRGPPDPLHGDALYKAVREAAQELLEALFSD 119
PHA03247 super family cl33720
large tegument protein UL36; Provisional
 2-150 2.32e-03

large tegument protein UL36; Provisional


The actual alignment was detected with superfamily member PHA03247:

Pssm-ID: 223021 [Multi-domain]  Cd Length: 3151  Bit Score: 41.46  E-value: 2.32e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636    2 AAAPPLRDRLSFLHRVSEGGPPGPRSHGSSraPHSPDSPRAGAIDLASPRRVAAPGPFARADAPELPA-RRAVPGPSKPG 80
Cdd:PHA03247 2687 AARPTVGSLTSLADPPPPPPTPEPAPHALV--SATPLPPGPAAARQASPALPAAPAPPAVPAGPATPGgPARPARPPTTA 2764

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636   81 LPSRTRRAQGRWAQPDPGGtnrPRPAFSGLRREGRSGGPGGRGAAVSVLLAPRWAGSPPSSLTVAFQRTP 150
Cdd:PHA03247 2765 GPPAPAPPAAPAAGPPRRL---TRPAVASLSESRESLPSPWDPADPPAAVLAPAAALPPAASPAGPLPPP 2831
 
Name Accession Description Interval E-value
ENTH_like_Tepsin cd03572
Epsin N-Terminal Homology (ENTH)-like domain of AP-4 complex accessory subunit Tepsin and ...
 227-346 2.07e-52

Epsin N-Terminal Homology (ENTH)-like domain of AP-4 complex accessory subunit Tepsin and similar domains; This family is composed of proteins containing an ENTH-like domain including vertebrate AP-4 complex accessory subunit Tepsin and Arabidopsis thaliana VHS domain-containing protein At3g16270. Tepsin is also called ENTH Domain-containing protein 2 (ENTHD2), Epsin for AP-4, or Tetra-epsin. It associates with the adapter-like complex 4 (AP-4), a heterotetramer composed of two large adaptins (epsilon and beta), a medium adaptin (mu) and a small adaptin (sigma), which forms a non-clathrin coat on vesicles departing the Trans-Golgi Network. The Epsin N-Terminal Homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. ENTH domain is highly similar to the N-terminal region of the AP180 N-Terminal Homology (ANTH_N) domain. ENTH and ANTH_N domains are structurally similar to the VHS domain and are composed of a superhelix of eight alpha helices. ENTH domains bind both, inositol phospholipids with preference for PtdIns(4,5)P2, and proteins, and contribute to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. ENTH and ANTH (E/ANTH)-containing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.


Pssm-ID: 340773  Cd Length: 119  Bit Score: 177.36  E-value: 2.07e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636 227 LPILLKGTSDDDVPCPGYLFEEIAtaEISHESPGSSQCLLEYLLSRLHSSSGHGKLKVLKILLYLCSHGSSFFLLILKRN 306
Cdd:cd03572    2 RPLLDKATSDDDEPTPGYLLEEIA--KLTRSSPGSCQELLDYLLKRLKKSSPHVKLKALRIIKHLCQKGSPEFRRELQRN 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 767993636 307 SAFIQEAAAFAGPPDPLHGNSLYQKVRAAAQDLGSTLFSD 346
Cdd:cd03572   80 SAAIRECTSYRGPPDPLHGDALYKAVREAAQELLEALFSD 119
PHA03247 PHA03247
large tegument protein UL36; Provisional
 2-150 2.32e-03

large tegument protein UL36; Provisional


Pssm-ID: 223021 [Multi-domain]  Cd Length: 3151  Bit Score: 41.46  E-value: 2.32e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636    2 AAAPPLRDRLSFLHRVSEGGPPGPRSHGSSraPHSPDSPRAGAIDLASPRRVAAPGPFARADAPELPA-RRAVPGPSKPG 80
Cdd:PHA03247 2687 AARPTVGSLTSLADPPPPPPTPEPAPHALV--SATPLPPGPAAARQASPALPAAPAPPAVPAGPATPGgPARPARPPTTA 2764

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636   81 LPSRTRRAQGRWAQPDPGGtnrPRPAFSGLRREGRSGGPGGRGAAVSVLLAPRWAGSPPSSLTVAFQRTP 150
Cdd:PHA03247 2765 GPPAPAPPAAPAAGPPRRL---TRPAVASLSESRESLPSPWDPADPPAAVLAPAAALPPAASPAGPLPPP 2831
 
Name Accession Description Interval E-value
ENTH_like_Tepsin cd03572
Epsin N-Terminal Homology (ENTH)-like domain of AP-4 complex accessory subunit Tepsin and ...
 227-346 2.07e-52

Epsin N-Terminal Homology (ENTH)-like domain of AP-4 complex accessory subunit Tepsin and similar domains; This family is composed of proteins containing an ENTH-like domain including vertebrate AP-4 complex accessory subunit Tepsin and Arabidopsis thaliana VHS domain-containing protein At3g16270. Tepsin is also called ENTH Domain-containing protein 2 (ENTHD2), Epsin for AP-4, or Tetra-epsin. It associates with the adapter-like complex 4 (AP-4), a heterotetramer composed of two large adaptins (epsilon and beta), a medium adaptin (mu) and a small adaptin (sigma), which forms a non-clathrin coat on vesicles departing the Trans-Golgi Network. The Epsin N-Terminal Homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. ENTH domain is highly similar to the N-terminal region of the AP180 N-Terminal Homology (ANTH_N) domain. ENTH and ANTH_N domains are structurally similar to the VHS domain and are composed of a superhelix of eight alpha helices. ENTH domains bind both, inositol phospholipids with preference for PtdIns(4,5)P2, and proteins, and contribute to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. ENTH and ANTH (E/ANTH)-containing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.


Pssm-ID: 340773  Cd Length: 119  Bit Score: 177.36  E-value: 2.07e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636 227 LPILLKGTSDDDVPCPGYLFEEIAtaEISHESPGSSQCLLEYLLSRLHSSSGHGKLKVLKILLYLCSHGSSFFLLILKRN 306
Cdd:cd03572    2 RPLLDKATSDDDEPTPGYLLEEIA--KLTRSSPGSCQELLDYLLKRLKKSSPHVKLKALRIIKHLCQKGSPEFRRELQRN 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 767993636 307 SAFIQEAAAFAGPPDPLHGNSLYQKVRAAAQDLGSTLFSD 346
Cdd:cd03572   80 SAAIRECTSYRGPPDPLHGDALYKAVREAAQELLEALFSD 119
VHS_ENTH_ANTH cd00197
VHS, ENTH and ANTH domain superfamily; This superfamily is composed of proteins containing a ...
 229-345 4.14e-04

VHS, ENTH and ANTH domain superfamily; This superfamily is composed of proteins containing a VHS, CID, ENTH, or ANTH domain. The VHS domain is present in Vps27 (Vacuolar Protein Sorting), Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) and STAM (Signal Transducing Adaptor Molecule). It is located at the N-termini of proteins involved in intracellular membrane trafficking. The CTD-Interacting Domain (CID) is present in several RNA-processing factors and binds tightly to the carboxy-terminal domain (CTD) of RNA polymerase II (RNAP II or Pol II). The epsin N-terminal homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. A set of proteins previously designated as harboring an ENTH domain in fact contains a highly similar, yet unique module referred to as an AP180 N-Terminal Homology (ANTH) domain. VHS, ENTH, and ANTH domains are structurally similar and are composed of a superhelix of eight alpha helices. ENTH and ANTH (E/ANTH) domains bind both inositol phospholipids and proteins and contribute to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. E/ANTH domain-bearing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.


Pssm-ID: 340764  Cd Length: 115  Bit Score: 40.49  E-value: 4.14e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636 229 ILLKGTSDDDVPCPGYLFEEIAtaEISHESPGSSQCLLEYLLSRLHSSSGHGKLKVLKILLYLCSHGSSFFLLILKRNSa 308
Cdd:cd00197    4 TVEKATSNENMGPDWPLIMEIC--DLINETNVGPKEAVDAIKKRINNKNPHVVLKALTLLEYCVKNCGERFHQEVASND- 80

                         90       100       110
                 ....*....|....*....|....*....|....*..
gi 767993636 309 FIQEAAAFagPPDPLHGNSLYQKVRAAAQDLGSTLFS 345
Cdd:cd00197   81 FAVELLKF--DKSGLLGDDVSTNVREKAIELVQLWAS 115
PHA03247 PHA03247
large tegument protein UL36; Provisional
 2-150 2.32e-03

large tegument protein UL36; Provisional


Pssm-ID: 223021 [Multi-domain]  Cd Length: 3151  Bit Score: 41.46  E-value: 2.32e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636    2 AAAPPLRDRLSFLHRVSEGGPPGPRSHGSSraPHSPDSPRAGAIDLASPRRVAAPGPFARADAPELPA-RRAVPGPSKPG 80
Cdd:PHA03247 2687 AARPTVGSLTSLADPPPPPPTPEPAPHALV--SATPLPPGPAAARQASPALPAAPAPPAVPAGPATPGgPARPARPPTTA 2764

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636   81 LPSRTRRAQGRWAQPDPGGtnrPRPAFSGLRREGRSGGPGGRGAAVSVLLAPRWAGSPPSSLTVAFQRTP 150
Cdd:PHA03247 2765 GPPAPAPPAAPAAGPPRRL---TRPAVASLSESRESLPSPWDPADPPAAVLAPAAALPPAASPAGPLPPP 2831
PRK07764 PRK07764
DNA polymerase III subunits gamma and tau; Validated
 1-145 5.99e-03

DNA polymerase III subunits gamma and tau; Validated


Pssm-ID: 236090 [Multi-domain]  Cd Length: 824  Bit Score: 39.97  E-value: 5.99e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636   1 MAAAPPLRDRLSFLHRVSEGGPPGPRSHGSSRAPHSPDspragaidlASPRRVAAPGPFARADAPELPARRAVPGPSKPG 80
Cdd:PRK07764 375 LARLERLERRLGVAGGAGAPAAAAPSAAAAAPAAAPAP---------AAAAPAAAAAPAPAAAPQPAPAPAPAPAPPSPA 445

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 767993636  81 LPSRTRRAQGRWAQPDPGGTNRPRPAFSGLRREGRSGGPGGRGAAVSVLLAPRWAGSPPSSLTVA 145
Cdd:PRK07764 446 GNAPAGGAPSPPPAAAPSAQPAPAPAAAPEPTAAPAPAPPAAPAPAAAPAAPAAPAAPAGADDAA 510
ENTH cd03571
Epsin N-Terminal Homology (ENTH) domain family; The Epsin N-Terminal Homology (ENTH) domain is ...
 234-316 8.52e-03

Epsin N-Terminal Homology (ENTH) domain family; The Epsin N-Terminal Homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. ENTH domain is highly similar to the N-terminal region of the AP180 N-Terminal Homology (ANTH_N) domain. ENTH and ANTH_N domains are structurally similar to the VHS domain and are composed of a superhelix of eight alpha helices. ENTH domains bind both, inositol phospholipids with preference for PtdIns(4,5)P2, and proteins, contributing to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. ENTH and ANTH (E/ANTH)-containing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.


Pssm-ID: 340772  Cd Length: 117  Bit Score: 36.73  E-value: 8.52e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767993636 234 TSDDDVPCPGYLFEEIATAeiSHESPGSSQcLLEYLLSRLhssSGHGK-----LKVLKILLYLCSHGSSFFLLILKRNSA 308
Cdd:cd03571    9 TSNEPWGPTGSQLAEIAQA--TFDYDDYQR-IMKVLWKRL---NDKGKnwrhvYKALTLLEYLLKNGSERVVDEFRDNLY 82


                 ....*...
gi 767993636 309 FIQEAAAF 316
Cdd:cd03571   83 LIRTLQDF 90
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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