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Conserved domains on  [gi|1034660683|ref|XP_011515431|]
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deubiquitinase OTUD6B isoform X1 [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
OTU_OTUD6 cd22761
OTU (ovarian tumor) domain of OTU domain-containing proteins 6A and 6B and similar proteins; ...
 37-181 8.76e-100

OTU (ovarian tumor) domain of OTU domain-containing proteins 6A and 6B and similar proteins; This subfamily is composed of mammalian OTU domain-containing protein 6A (OTUD6A, also called DUBA-2) and vertebrate OTU domain-containing protein 6B (OTUD6B, also called DUBA-5), which are deubiquitinating enzymes/ubiquitinyl hydrolases (EC 3.4.19.12). OTUD6A hydrolyzes 'Lys-27'-, 'Lys-29'-, and 'Lys-33'-linked polyubiquitin chains, and may also be able to hydrolyze 'Lys-11'-linked ubiquitin chains. It deubiquitylates and stabilizes dynamin-related protein 1 (Drp1), a cytosolic protein responsible for mitochondrial fission and is essential in the initiation and development of several human diseases including cancer, thereby facilitating tumorigenesis. OTUD6B is a functional deubiquitinase in in vitro enzyme assays. It may play a role in the ubiquitin-dependent regulation of protein synthesis downstream of mTORC1, and may modify the ubiquitination of the protein synthesis initiation complex to repress translation. Biallelic variants in OTUD6B cause an intellectual disability syndrome that is associated with seizures and dysmorphic features. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


:

Pssm-ID: 438598 [Multi-domain]  Cd Length: 146  Bit Score: 284.39  E-value: 8.76e-100
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  37 LAQILAARQLEIKQIPSDGHCMYKAIEDQLKEKDCALTVVALRSQTAEYMQSHVEDFLPFLTNPNTGDMYTPEEFQKYCE 116
Cdd:cd22761     1 IKKILKERGLKIHEIPSDGDCLYNAIAHQLSLRGIETSVEELRKQTADYMRENKDDFLPFLTNPDTGDPLTEEEFEKYCD 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1034660683 117 DIVNTAAWGGQLELRALSHILQTPIEIIQADSPPIIVGEEY-SKKPLILVYMRHAYGLGEHYNSVT 181
Cdd:cd22761    81 DVENTGAWGGQLELRALSHVLKRPIEVIQAEGPPIIIGEEFkSGKPLILTYHRHAYGLGEHYNSVE 146
 
Name Accession Description Interval E-value
OTU_OTUD6 cd22761
OTU (ovarian tumor) domain of OTU domain-containing proteins 6A and 6B and similar proteins; ...
 37-181 8.76e-100

OTU (ovarian tumor) domain of OTU domain-containing proteins 6A and 6B and similar proteins; This subfamily is composed of mammalian OTU domain-containing protein 6A (OTUD6A, also called DUBA-2) and vertebrate OTU domain-containing protein 6B (OTUD6B, also called DUBA-5), which are deubiquitinating enzymes/ubiquitinyl hydrolases (EC 3.4.19.12). OTUD6A hydrolyzes 'Lys-27'-, 'Lys-29'-, and 'Lys-33'-linked polyubiquitin chains, and may also be able to hydrolyze 'Lys-11'-linked ubiquitin chains. It deubiquitylates and stabilizes dynamin-related protein 1 (Drp1), a cytosolic protein responsible for mitochondrial fission and is essential in the initiation and development of several human diseases including cancer, thereby facilitating tumorigenesis. OTUD6B is a functional deubiquitinase in in vitro enzyme assays. It may play a role in the ubiquitin-dependent regulation of protein synthesis downstream of mTORC1, and may modify the ubiquitination of the protein synthesis initiation complex to repress translation. Biallelic variants in OTUD6B cause an intellectual disability syndrome that is associated with seizures and dysmorphic features. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438598 [Multi-domain]  Cd Length: 146  Bit Score: 284.39  E-value: 8.76e-100
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  37 LAQILAARQLEIKQIPSDGHCMYKAIEDQLKEKDCALTVVALRSQTAEYMQSHVEDFLPFLTNPNTGDMYTPEEFQKYCE 116
Cdd:cd22761     1 IKKILKERGLKIHEIPSDGDCLYNAIAHQLSLRGIETSVEELRKQTADYMRENKDDFLPFLTNPDTGDPLTEEEFEKYCD 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1034660683 117 DIVNTAAWGGQLELRALSHILQTPIEIIQADSPPIIVGEEY-SKKPLILVYMRHAYGLGEHYNSVT 181
Cdd:cd22761    81 DVENTGAWGGQLELRALSHVLKRPIEVIQAEGPPIIIGEEFkSGKPLILTYHRHAYGLGEHYNSVE 146
OTU pfam02338
OTU-like cysteine protease; This family is comprised of a group of predicted cysteine ...
 52-177 1.93e-45

OTU-like cysteine protease; This family is comprised of a group of predicted cysteine proteases, homologous to the Ovarian Tumour (OTU) gene in Drosophila. Members include proteins from eukaryotes, viruses and pathogenic bacterium. The conserved cysteine and histidine, and possibly the aspartate, represent the catalytic residues in this putative group of proteases.


Pssm-ID: 426728 [Multi-domain]  Cd Length: 127  Bit Score: 146.44  E-value: 1.93e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  52 PSDGHCMYKAIEDQL---KEKDCALTVVALRSQTAEYMQSHVEDFLPFLTNPNTGDMYtpeefqkyceDIVNTAAWGGQL 128
Cdd:pfam02338   1 PGDGNCLYRSISHQLwgvHDVLRKMLVQELRETLAEYMREHKEEFEPFLEDDETGDII----------EIEQTGAWGGEI 70

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1034660683 129 ELRALSHILQTPIEIIQADSPPIIVG-EEYS-------KKPLILVYMRHAYGLGEHY 177
Cdd:pfam02338  71 EIFALAHILRRPIIVYKSEGGEELGGlKEYGiylplgwDPSLCLVYPRHLYYLGGHY 127
COG5539 COG5539
Predicted cysteine protease (OTU family) [Posttranslational modification, protein turnover, ...
 32-179 4.58e-18

Predicted cysteine protease (OTU family) [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 227826 [Multi-domain]  Cd Length: 306  Bit Score: 79.92  E-value: 4.58e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  32 MESEKLAQILAARQLEIKQIPSDGHCMYKAIEDQLKEKDCAL---TVVALRSQTAEYMQSHVEDFLPFLTNPNTGDMytp 108
Cdd:COG5539   157 PAILEIDVIAYATWIVKPDSQGDGCIEIAIISDQLPVRIHVVdvdKDSEDRYNSHPYVQRISILFTGIHFDEETLAM--- 233

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1034660683 109 EEFQKYCEDIVNTAAWGGQLELRALSHILQTPIEIIQADSPPIIVGEEYSKKPLILVYMRHAYGLGeHYNS 179
Cdd:COG5539   234 VLWDTYVNEVLFDASDGITIEIQQLASLLKNPHYYTNTASPSIKCNICGTGFVGEKDYYAHALATG-HYNF 303
 
Name Accession Description Interval E-value
OTU_OTUD6 cd22761
OTU (ovarian tumor) domain of OTU domain-containing proteins 6A and 6B and similar proteins; ...
 37-181 8.76e-100

OTU (ovarian tumor) domain of OTU domain-containing proteins 6A and 6B and similar proteins; This subfamily is composed of mammalian OTU domain-containing protein 6A (OTUD6A, also called DUBA-2) and vertebrate OTU domain-containing protein 6B (OTUD6B, also called DUBA-5), which are deubiquitinating enzymes/ubiquitinyl hydrolases (EC 3.4.19.12). OTUD6A hydrolyzes 'Lys-27'-, 'Lys-29'-, and 'Lys-33'-linked polyubiquitin chains, and may also be able to hydrolyze 'Lys-11'-linked ubiquitin chains. It deubiquitylates and stabilizes dynamin-related protein 1 (Drp1), a cytosolic protein responsible for mitochondrial fission and is essential in the initiation and development of several human diseases including cancer, thereby facilitating tumorigenesis. OTUD6B is a functional deubiquitinase in in vitro enzyme assays. It may play a role in the ubiquitin-dependent regulation of protein synthesis downstream of mTORC1, and may modify the ubiquitination of the protein synthesis initiation complex to repress translation. Biallelic variants in OTUD6B cause an intellectual disability syndrome that is associated with seizures and dysmorphic features. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438598 [Multi-domain]  Cd Length: 146  Bit Score: 284.39  E-value: 8.76e-100
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  37 LAQILAARQLEIKQIPSDGHCMYKAIEDQLKEKDCALTVVALRSQTAEYMQSHVEDFLPFLTNPNTGDMYTPEEFQKYCE 116
Cdd:cd22761     1 IKKILKERGLKIHEIPSDGDCLYNAIAHQLSLRGIETSVEELRKQTADYMRENKDDFLPFLTNPDTGDPLTEEEFEKYCD 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1034660683 117 DIVNTAAWGGQLELRALSHILQTPIEIIQADSPPIIVGEEY-SKKPLILVYMRHAYGLGEHYNSVT 181
Cdd:cd22761    81 DVENTGAWGGQLELRALSHVLKRPIEVIQAEGPPIIIGEEFkSGKPLILTYHRHAYGLGEHYNSVE 146
OTU_OTUD6-like cd22748
OTU (ovarian tumor) domain of OTU domain-containing proteins 6A, 6B, and similar proteins; ...
 41-180 8.96e-79

OTU (ovarian tumor) domain of OTU domain-containing proteins 6A, 6B, and similar proteins; This subfamily is composed of mammalian OTU domain-containing protein 6A (OTUD6A, also called DUBA-2, vertebrate OTU domain-containing protein 6B (OTUD6B, also called DUBA-5), fungal OTU domain-containing protein 2 (OTU2), and similar proteins. OTUD6A, OTUD6B, and Schizosaccharomyces pombe OTU2 are deubiquitinating enzymes/ubiquitinyl hydrolases (EC 3.4.19.12). OTUD6A hydrolyzes 'Lys-27'-, 'Lys-29'-, and 'Lys-33'-linked polyubiquitin chains, and may also be able to hydrolyze 'Lys-11'-linked ubiquitin chains. It deubiquitylates and stabilizes dynamin-related protein 1 (Drp1), a cytosolic protein responsible for mitochondrial fission and is essential in the initiation and development of several human diseases including cancer, thereby facilitating tumorigenesis. OTUD6B is a functional deubiquitinase in in vitro enzyme assays. It may play a role in the ubiquitin-dependent regulation of protein synthesis downstream of mTORC1, and may modify the ubiquitination of the protein synthesis initiation complex to repress translation. Biallelic variants in OTUD6B cause an intellectual disability syndrome that is associated with seizures and dysmorphic features. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438585 [Multi-domain]  Cd Length: 144  Bit Score: 231.30  E-value: 8.96e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  41 LAARQLEIKQIPSDGHCMYKAIEDQLKE---KDCALTVVALRSQTAEYMQSHVEDFLPFLTNpNTGDMYTPEEFQKYCED 117
Cdd:cd22748     1 LKPLGLRIKEIPPDGHCLYRAIADQLKLrggSEEPYSYKELRKLAADYMRAHRDDFLPFLTN-DDGDLMTEEEFEEYCDK 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1034660683 118 IVNTAAWGGQLELRALSHILQTPIEIIQADSPPIIVGEEY-SKKPLILVYMRHAYGLGEHYNSV 180
Cdd:cd22748    80 IENTAEWGGQLELRALSKALKRPIHVYQAGSPPLVIGEEFdSGEPLRLSYHRHAYGLGEHYNSV 143
OTU_plant_OTU5-like cd22797
OTU (ovarian tumor) domain of deubiquitinating enzyme OTU5 from plants and similar proteins; ...
 37-180 1.72e-53

OTU (ovarian tumor) domain of deubiquitinating enzyme OTU5 from plants and similar proteins; Deubiquitinating enzyme OTU5, also called OTU domain-containing protein 6, is a deubiquitinase (DUB) or ubiquitin thiolesterase (EC 3.4.19.12) that catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU5 is an inactive cysteine protease. It regulates gene expression by contributing to chromatin organization and DNA methylation patterns (e.g. H3K4me3 and H3K27me3). It is required for phosphate (Pi) homeostasis. OTU5 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438618 [Multi-domain]  Cd Length: 149  Bit Score: 167.52  E-value: 1.72e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  37 LAQILAARQLEIKQIPSDGHCMYKAIEDQLKEK---DCALTVVALRSQTAEYMQSHVEDFLPFLTNPNTGDmyTPEE-FQ 112
Cdd:cd22797     1 LRAKLAPLGLAIKEIKADGHCLYRAVEDQLQLRgggAPAPDYQQLRELAADYMRAHPDDFLPFLEDEDEGG--DGDEaFE 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683 113 KYCEDIVNTAAWGGQLELRALSHILQTPIEIIQADSPPIIVGEEYSKK--PLILVYMRHAYGLGEHYNSV 180
Cdd:cd22797    79 AYCREVESTAAWGGQLELGALAHALRRHIKVYSAGMPDVEMGEEYAGTgpPLRLCYHRHAFGLGEHYNSV 148
OTU_fungi_OTU2-like cd22762
OTU (ovarian tumor) domain of fungal OTU domain-containing protein 2 and similar proteins; ...
 40-180 3.55e-51

OTU (ovarian tumor) domain of fungal OTU domain-containing protein 2 and similar proteins; This subfamily includes Schizosaccharomyces pombe and Saccharomyces cerevisiae OTU domain-containing protein 2 (OTU2) and similar proteins. S. pombe OTU2 is a ubiquitin thioesterase/hydrolase (EC 3.4.19.12) that can remove conjugated ubiquitin from protein substrates and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Fungal OTU2 bbelongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438599 [Multi-domain]  Cd Length: 142  Bit Score: 161.24  E-value: 3.55e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  40 ILAARQLEIKQIPSDGHCMYKAIEDQLKEKDCA--LTVVALRSQTAEYMQSHVEDFLPFLTNpNTGDmytPEEFQKYCED 117
Cdd:cd22762     1 LLEELGLEEHDIKPDGHCLFAAIADQLQLRGSEinLDYKELRKLAAEYIRKHPDDFEPFLFE-ETDE---LEDIDEYCKK 76

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1034660683 118 IVNTAAWGGQLELRALSHILQTPIEIIQADSPPIIVGEE--YSKKPLILVYMRHAYGLGEHYNSV 180
Cdd:cd22762    77 IENTAEWGGELELLALAKAFGVPIHVVQAEGRVIKINEEgdSDKPELWLAYYKHSYGLGEHYNSL 141
OTU pfam02338
OTU-like cysteine protease; This family is comprised of a group of predicted cysteine ...
 52-177 1.93e-45

OTU-like cysteine protease; This family is comprised of a group of predicted cysteine proteases, homologous to the Ovarian Tumour (OTU) gene in Drosophila. Members include proteins from eukaryotes, viruses and pathogenic bacterium. The conserved cysteine and histidine, and possibly the aspartate, represent the catalytic residues in this putative group of proteases.


Pssm-ID: 426728 [Multi-domain]  Cd Length: 127  Bit Score: 146.44  E-value: 1.93e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  52 PSDGHCMYKAIEDQL---KEKDCALTVVALRSQTAEYMQSHVEDFLPFLTNPNTGDMYtpeefqkyceDIVNTAAWGGQL 128
Cdd:pfam02338   1 PGDGNCLYRSISHQLwgvHDVLRKMLVQELRETLAEYMREHKEEFEPFLEDDETGDII----------EIEQTGAWGGEI 70

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1034660683 129 ELRALSHILQTPIEIIQADSPPIIVG-EEYS-------KKPLILVYMRHAYGLGEHY 177
Cdd:pfam02338  71 EIFALAHILRRPIIVYKSEGGEELGGlKEYGiylplgwDPSLCLVYPRHLYYLGGHY 127
OTU_plant_OTU7-like cd22771
OTU (ovarian tumor) domain of Arabidopsis thaliana deubiquitinating enzyme OTU7 and similar ...
 46-180 2.48e-29

OTU (ovarian tumor) domain of Arabidopsis thaliana deubiquitinating enzyme OTU7 and similar proteins; Arabidopsis thaliana deubiquitinating enzyme OTU7, also called OTU domain-containing protein 7, is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that shows a preference for 'Lys-63' over 'Lys-48' over 'Met-1'-linked ubiquitin (UB) tetramers as substrates. DUBs catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. OTU7 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438608 [Multi-domain]  Cd Length: 124  Bit Score: 104.94  E-value: 2.48e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  46 LEIKQIPSDGHCMYKAIEDQL--KEKDCAltvvALRSQTAEYMQSHVEDFLPFltnpntgdMYTPEEFQKYCEDIVNTAA 123
Cdd:cd22771     2 LRIRDVEGDGNCLFRALADQLygDEERHA----ELRKKVVDYMEAHEEDFEPF--------FEDDETFEDYVSRMREDGT 69

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1034660683 124 WGGQLELRALSHILQTPIEIIQADSPPIIVG--EEYSKKPLILVYmrHAyglGEHYNSV 180
Cdd:cd22771    70 WGGNLELQAASLVYRVNIVVHQLGQPRWEIEnfPDKGARTIHLSY--HD---GEHYNSV 123
OTU cd22744
OTU (ovarian tumor) domain family; The OTU family of cysteine proteases use a conserved ...
 47-180 3.20e-24

OTU (ovarian tumor) domain family; The OTU family of cysteine proteases use a conserved cysteine and histidine, and in most cases an aspartate, as the catalytic triad. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation.


Pssm-ID: 438581 [Multi-domain]  Cd Length: 128  Bit Score: 92.11  E-value: 3.20e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  47 EIKQIPSDGHCMYKAIEDQLKEKDCalTVVALRSQTAEYMQSHVEDFLPFltnpNTGDMYTPEEFQKYCEDIVNTAAWGG 126
Cdd:cd22744     1 RVVDVPGDGNCLFRALAHALYGDQE--SHRELRQEVVDYLRENPDLYEPA----ELADEDDGEDFDEYLQRMRKPGTWGG 74

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1034660683 127 QLELRALSHILQTPIEIIQADS---PPIIVGEEY--SKKPLILVYMRhayglGEHYNSV 180
Cdd:cd22744    75 ELELQALANALNVPIVVYSEDGgflPVSVFGPGPgpSGRPIHLLYTG-----GNHYDAL 128
OTU_OTUD3-like cd22756
OTU (ovarian tumor) domain of OTU domain-containing protein 3 and similar proteins; This ...
 48-180 1.64e-19

OTU (ovarian tumor) domain of OTU domain-containing protein 3 and similar proteins; This subfamily includes bilaterial OTU domain-containing protein 3 (OTUD3), Arabidopsis thaliana deubiquitinating enzyme OTU7, also called OTU domain-containing protein 7, and similar proteins. OTUD3 is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It is an acetylation-dependent deubiquitinase that restricts innate antiviral immune signaling. It directly hydrolyzes lysine 63 (Lys63)-linked polyubiquitination of MAVS (mitochondrial antiviral-signaling protein) and shuts off innate antiviral immune response. OTUD3 can elicit tumor-suppressing or tumor-promoting activities in a cell- and tissue-dependent manner. It is a DUB for PTEN (phosphatase and tension homologue deleted on chromosome 10); the OTUD3-PTEN signaling axis plays a critical role in suppression of breast tumorigenesis. OTUD3 is also a DUB for glucose-regulated protein GRP78, stabilizing it and promoting lung tumorigenesis. OTU7 is a DUB that shows a preference for 'Lys-63' over 'Lys-48' over 'Met-1'-linked ubiquitin (UB) tetramers as substrates. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438593 [Multi-domain]  Cd Length: 131  Bit Score: 79.91  E-value: 1.64e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  48 IKQIPSDGHCMYKAIEDQL-------KEkdcaltvvaLRSQTAEYMQSHVEDFLPFLTNPNTGDMytPEEFQKYCEDIVN 120
Cdd:cd22756     2 AKDITGDGNCLFRALSDQLygdpdrhLE---------IRAEVVEYMRANPDDFKPFSEAATFAED--DEAFEDYLARMAK 70

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1034660683 121 TAAWGGQLELRALSHILQTPIEIIQADSPPIIVGEEY-----SKKPLILVYmrHAYglgEHYNSV 180
Cdd:cd22756    71 DGTYGDNLEIVAFARAYNVDVKVYQPDPVYVISAPEDgspgpARRVLHIAY--HNW---EHYSSV 130
OTU_232R-like cd22758
OTU (ovarian tumor) domain of Invertebrate iridescent virus putative ubiquitin thioesterase ...
 46-180 1.91e-18

OTU (ovarian tumor) domain of Invertebrate iridescent virus putative ubiquitin thioesterase 232R and similar proteins; This subfamily contains putative ubiquitin thioesterases 232R from Invertebrate iridescent virus and L96 from Tipula iridescent virus (TIV), Dictyostelium discoideum OTU domain-containing protein DDB_G0284757, and similar proteins. L96 may be involved in TIV genomic DNA packaging in a manner related to the Gag polyproteins of the mammalian viruses. Proteins in this subfamily contain an OTU domain. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. They belong to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438595 [Multi-domain]  Cd Length: 135  Bit Score: 77.31  E-value: 1.91e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  46 LEIKQIPSDGHCMYKAIEDQLKEKDCALTVVALRSQTAEYMQSHVEDFLPFLTNPNTGDmytpEEFQKYCEDIVNTAAWG 125
Cdd:cd22758     6 FEIRDVPGDGNCFFHAVSDQLYGNGIEHSHKELRQQAVNYLRENPELYDGFFLSEFDEE----ESWEEYLNRMSKDGTWG 81

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1034660683 126 GQLELRALSHILQTPIEIIQAD--SPPIIV--GEEYSKKPLILVYMRhayglGEHYNSV 180
Cdd:cd22758    82 DHIILQAAANLFNVRIVIISSDgsDETTIIepGNSKNGRTIYLGHIG-----ENHYVSL 135
COG5539 COG5539
Predicted cysteine protease (OTU family) [Posttranslational modification, protein turnover, ...
 32-179 4.58e-18

Predicted cysteine protease (OTU family) [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 227826 [Multi-domain]  Cd Length: 306  Bit Score: 79.92  E-value: 4.58e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  32 MESEKLAQILAARQLEIKQIPSDGHCMYKAIEDQLKEKDCAL---TVVALRSQTAEYMQSHVEDFLPFLTNPNTGDMytp 108
Cdd:COG5539   157 PAILEIDVIAYATWIVKPDSQGDGCIEIAIISDQLPVRIHVVdvdKDSEDRYNSHPYVQRISILFTGIHFDEETLAM--- 233

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1034660683 109 EEFQKYCEDIVNTAAWGGQLELRALSHILQTPIEIIQADSPPIIVGEEYSKKPLILVYMRHAYGLGeHYNS 179
Cdd:COG5539   234 VLWDTYVNEVLFDASDGITIEIQQLASLLKNPHYYTNTASPSIKCNICGTGFVGEKDYYAHALATG-HYNF 303
OTU_OTUD5-like cd22752
OTU (ovarian tumor) domain of OTU domain-containing protein 5 and similar proteins; OTU ...
 45-180 1.89e-15

OTU (ovarian tumor) domain of OTU domain-containing protein 5 and similar proteins; OTU domain-containing protein 5 (OTUD5), also called deubiquitinating enzyme A (DUBA), is a phosphorylation-dependent deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that can hydrolyze 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, and may function as negative regulator of the innate immune system. It limits type I interferon production in macrophages and suppresses interleukin-17A production in T cells. OTUD5 also functions in an apoptotic signaling cascade by mediating the sequential activation of PDCD5 (programmed cell death 5) and p53 in response to genotoxic stress. In Drosophila, OTUD5/DUBA is essential for spermatogenesis. This subfamily also includes Arabidopsis thaliana OTU domain-containing protein 6, also called deubiquitinating enzyme OTU6 or otubain-like deubiquitinase 1 (OTLD1), which binds chromatin and has enzymatic histone deubiquitinase activity specific for the H2B histone. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It also includes plant OTU6.


Pssm-ID: 438589 [Multi-domain]  Cd Length: 124  Bit Score: 69.11  E-value: 1.89e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  45 QLEIKQIPSDGHCMYKAIEDQLKEKDCALTVValRSQTAEYMQSHVEDFLPFLTnpntgdmytpEEFQKYCEDIVNTAAW 124
Cdd:cd22752     1 GFIIKEMEEDGNCLFRAVADQVYGDQEMHDVV--RKHCMDYMEKNRDYFSQFVT----------EDFEEYINRKRQDGVW 68

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1034660683 125 GGQLELRALSHILQTPIEIIQADSPPIIVGEEYSKK---PLILVYMRHAyglgeHYNSV 180
Cdd:cd22752    69 GNHIEIQAMSELYNRPIEVYAYSTEPINTFHEASSSdnePIRLSYHGNS-----HYNSI 122
OTU_OTUD3 cd22770
OTU (ovarian tumor) domain of OTU domain-containing protein 3 and similar proteins; OTU ...
 37-183 9.64e-14

OTU (ovarian tumor) domain of OTU domain-containing protein 3 and similar proteins; OTU domain-containing protein 3 (OTUD3) is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It is an acetylation-dependent deubiquitinase that restricts innate antiviral immune signaling. It directly hydrolyzes lysine 63 (Lys63)-linked polyubiquitination of MAVS (mitochondrial antiviral-signaling protein) and shuts off innate antiviral immune response. OTUD3 can elicit tumor-suppressing or tumor-promoting activities in a cell- and tissue-dependent manner. It is a DUB for PTEN (phosphatase and tension homologue deleted on chromosome 10); the OTUD3-PTEN signaling axis plays a critical role in suppression of breast tumorigenesis. OTUD3 is also a DUB for glucose-regulated protein GRP78, stabilizing it and promoting lung tumorigenesis. It belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438607 [Multi-domain]  Cd Length: 145  Bit Score: 65.38  E-value: 9.64e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  37 LAQILAARQLEIKQIPSDGHCMYKAIEDQLkEKDCALTVVaLRSQTAEYMQSHVEDFLPFLTNPntgdmyTPeeFQKYCE 116
Cdd:cd22770     5 FANQLQALGLKLRDIPGDGNCLFRALGDQL-EGHSRNHLK-HRQETVQYMIEHREDFEPFVEDD------VP--FDKHVA 74

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1034660683 117 DIVNTAAWGGQLELRALSHILQTPIEIIQADSPP-IIVGEEY-SKKPLILvymrhAYGLGEHYNSVTRL 183
Cdd:cd22770    75 NLSKPGTYAGNDAIVAFARLHQVNVVIHQLNAPLwQIRGTEKsSSRELHI-----SYHNGDHYSSVRKL 138
OTU_plant_OTU6-like cd22796
OTU (ovarian tumor) domain of deubiquitinating enzyme OTU6 from plants and similar proteins; ...
 43-180 1.20e-13

OTU (ovarian tumor) domain of deubiquitinating enzyme OTU6 from plants and similar proteins; Deubiquitinating enzyme OTU6, also called OTU domain-containing protein 6 or otubain-like deubiquitinase 1 (OTLD1), is a deubiquitinase (DUB) or ubiquitin thiolesterase (EC 3.4.19.12) that catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU6 binds chromatin and has enzymatic histone deubiquitinase activity specific for the H2B histone. OTU6 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438617 [Multi-domain]  Cd Length: 128  Bit Score: 64.75  E-value: 1.20e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  43 ARQLEIKQIPSDGHCMYKAIEDQLKEKDCALTVValRSQTAEYMQSHVEDFLPFLTnpntgdmytpEEFQKYCEDIVNTA 122
Cdd:cd22796     2 KKGLEIRRMDGDGNCLFRAVADQVYGDQEMHDEV--REMCMDYMEKERDHFSQFVT----------EDFTQYVKRKRRDR 69

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1034660683 123 AWGGQLELRALSHILQTPIEIIQ--ADSPPIIVGEEYSKK--PLILVYMRhayglGEHYNSV 180
Cdd:cd22796    70 VFGNNLEIQAMSEIYNRPIEVYSysNGEPINIFHGSYEGDdpPIRLSYHD-----GNHYNSI 126
OTU_ALG13-like cd22753
OTU (ovarian tumor) domain of bifunctional UDP-N-acetylglucosamine transferase and ...
 39-180 4.14e-13

OTU (ovarian tumor) domain of bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 and similar proteins; Bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 is alco called asparagine-linked glycosylation 13 homolog, glycosyltransferase 28 domain-containing protein 1 (GLT28D1), or UDP-N-acetylglucosamine transferase subunit ALG13 homolog. It displays both glycosyltransferase (EC 2.4.1.141) and deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) activities. With ALG14, it forms a UDP-N-acetylglucosamine transferase that catalyzes the second step of eukaryotic N-linked glycosylation in the endoplasmic reticulum. ALG13 variants cause a form of early infantile epileptic encephalopathy known as EIEE36 refractory seizures, neurodevelopmental impairment, and poor prognosis; given the essential role of ALG13 in glycosylation, it is also considered a congenital disorder of glycosylation (CDG). This subfamily also contains OTU domain-containing protein 4 (OTUD4), also called HIV-1-induced protein HIN-1, a DUB that specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein upon phosphorylation, triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438590 [Multi-domain]  Cd Length: 130  Bit Score: 63.33  E-value: 4.14e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  39 QILAARQLEIKQIPSDGHCMYKAIEDQLkeKDCALTVVALRSQTAEYMQSHVEDFLPFLtnpntgdmytPEEFQKYCEDI 118
Cdd:cd22753     3 EYLDSLGLYRKHIPRDGSCLFRAVSEQL--FFTQSYHQQVRQACVEYLEKNREEFEKFS----------EISFDDYLERL 70

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1034660683 119 VNTAAWGGQLELRALSHILQTPIEIIQ-ADSPPIIVGEEYSKKPLILVYMrhaygLGEHYNSV 180
Cdd:cd22753    71 SDPKEWGGLLELEALSLLYKVDFIVYSiPDQPPSNITNNGYPKKIMLCYS-----GGNHYDSV 128
OTU_OTUD4 cd22794
OTU (ovarian tumor) domain of OTU domain-containing protein 4 and similar proteins; OTU ...
 41-180 2.32e-12

OTU (ovarian tumor) domain of OTU domain-containing protein 4 and similar proteins; OTU domain-containing protein 4 (OTUD4), also called HIV-1-induced protein HIN-1, is a deubiquitinase that specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein upon phosphorylation, triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators. OTUD4 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438615 [Multi-domain]  Cd Length: 130  Bit Score: 61.23  E-value: 2.32e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  41 LAARQLEIKQIPSDGHCMYKAIEDQLKekDCALTVVALRSQTAEYMQSHVEDFLPFLTNPntgdmytpeeFQKYCEDIVN 120
Cdd:cd22794     5 LRSLGLYRKQIAKDGSCLFRAVAEQVF--HTQSRHLEVRKACVDYLRRNREKFEAFIEGP----------FEQYLKNLEN 72

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1034660683 121 TAAWGGQLELRALSHILQTPIEIIQ-ADSPPIIVGEE-YSKKPLIlvymrhAYGLGEHYNSV 180
Cdd:cd22794    73 PKEWAGQVEISALSLMYKRDFIIYQePGKPPSNVTENgFPDKILL------CFSNGNHYDSV 128
OTU_plant_OTU4-like cd22760
OTU (ovarian tumor) domain of deubiquitinating enzyme OTU4 from plants and similar proteins; ...
 46-181 6.11e-12

OTU (ovarian tumor) domain of deubiquitinating enzyme OTU4 from plants and similar proteins; Deubiquitinating enzyme OTU4, also called OTU domain-containing protein 4, is a deubiquitinase (DUB) or ubiquitin thiolesterase (EC 3.4.19.12) that catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU4 may play an important regulatory role at the level of protein turnover by preventing degradation. OTU4 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438597 [Multi-domain]  Cd Length: 138  Bit Score: 60.47  E-value: 6.11e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  46 LEIKQIPSDGHCMYKAI---------------EDQLKEKDcaltvvALRSQTA-EYMQ--SHVEDFLpfltnpnTGDmyt 107
Cdd:cd22760     2 YRVHGIAGDGRCLFRAVahgeclargkaapdeERERELAD------ELRTRAAdELVKrrEETEWFI-------EGD--- 65

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683 108 peeFQKYCEDIVNTAAWGGQLELRALSHILQTPIEIIQAD--SPPII----VGEEYSKK-PLILVYmrHAYGlgeHYNSV 180
Cdd:cd22760    66 ---FDEYVARMRRPGVWGGEPELLMLSHVLQRPITVYMADegEGGLIsiaeYGQEYGKGnPIRVLF--HGFG---HYEAL 137


                  .
gi 1034660683 181 T 181
Cdd:cd22760   138 L 138
OTU_plant_OTU9-like cd22751
OTU (ovarian tumor) domain of plant deubiquitinating enzyme OTU9 and similar proteins; This ...
 37-181 2.81e-11

OTU (ovarian tumor) domain of plant deubiquitinating enzyme OTU9 and similar proteins; This subfamily contains Arabidopsis thaliana deubiquitinating enzymes OTU8, OTU9, OTU10, OTU11, and OTU12, and similar proteins from plants and other eukaryotes. OTU8-OTU12 are deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. They belong to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438588 [Multi-domain]  Cd Length: 134  Bit Score: 58.32  E-value: 2.81e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  37 LAQILAARQLEIKQIPSDGHCMYKAIEDQL-KEKDCALTVvalRSQTAEYMQSHVEDFLPFltnpntgdmYTPEEFQKYC 115
Cdd:cd22751     1 LLRRLDLYGLVERKVEGDGNCQFRALSDQLfGTQDHHAEV---RELVVKQLRAHPELYYEF---------YVPEEYDEYL 68

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1034660683 116 EDIVNTAAWGGQLELRALSHILQTPIEIIQ--ADSPPIIV---GEEYSKKPLILVYMRHayglgEHYNSVT 181
Cdd:cd22751    69 KKMSKDGEWGDELTLQAAADAFGVKIHVITsfEDNWFLEIeprGLVRSKRVLFLSYWAE-----VHYNSIY 134
OTU_OTUD1 cd22747
OTU (ovarian tumor) domain of OTU domain-containing protein 1 and similar proteins; OTU ...
 29-180 4.64e-11

OTU (ovarian tumor) domain of OTU domain-containing protein 1 and similar proteins; OTU domain-containing protein 1 (OTUD1), also called DUBA-7 in humans, is a deubiquitinating enzyme/ubiquitinyl hydrolase (EC 3.4.19.12) that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin; this specificity is facilitated by the C-terminal Ub-interacting motif (UIM) of OTUD1. It interacts and promotes the deubiquitination of myeloid cell leukemia 1 (MCL1), a pro-survival Bcl-2 family protein that plays important roles in cell survival, proliferation, differentiation and tumorigenesis. OTUD1 also deubiquitinates IFN regulatory factor 3 (IRF3) and attenuates its function; IRF3 is critical for the transcription of type I IFNs in defensing virus and promoting inflammatory responses. Loss-of-function mutations of OTUD1 associated with multiple autoimmune diseases including systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), ulcerative colitis (UC) and Hashimoto's thyroiditis (HT). OTUD1 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438584 [Multi-domain]  Cd Length: 149  Bit Score: 58.28  E-value: 4.64e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  29 ARHM-ESEKLAQILAARQLEIKQIPSDGHCMYKAIE-----DQLKEKDcaltvvaLRSQTAEYMQSHVEDFLPFLtnpnT 102
Cdd:cd22747     3 ALYLaEVEKQDKYLRERNKYRFHIIPDGNCLYRAVSkavygDQALHRE-------LREQTVHYIADHLDEFNPII----E 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683 103 GDMytpEEFqkycedIVNTA---AWGGQLELRALSHILQTPIEIIQADSP--PII------VGEEYSKKPLI-LVYMRHA 170
Cdd:cd22747    72 GDV---GEF------LIKAAqdgAWAGYPELLAMGQMLNVNIRLTTGGSLesPTVstmvhyLGPEDSGKPSIwLSWLSNG 142

                         170
                  ....*....|
gi 1034660683 171 yglgeHYNSV 180
Cdd:cd22747   143 -----HYDAV 147
OTU_plant_OTU3_4-like cd22746
OTU (ovarian tumor) domain of deubiquitinating enzymes OTU3 and OTU4 from plants, and similar ...
 45-177 2.13e-10

OTU (ovarian tumor) domain of deubiquitinating enzymes OTU3 and OTU4 from plants, and similar proteins; Deubiquitinating enzyme OTU3 (also called OTU domain-containing protein 3) and deubiquitinating enzyme OTU4 (also called OTU domain-containing protein 4) are deubiquitinases (DUBs) or ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU3 and OTU4 may play important regulatory roles at the level of protein turnover by preventing degradation. They belong to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438583 [Multi-domain]  Cd Length: 141  Bit Score: 56.12  E-value: 2.13e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  45 QLEIKQIPSDGHCMYKAI---------EDQLKEKDCALTVVALRSQTAEYMQSHVEDFLPfltnpntGDMYTPEEFQKYC 115
Cdd:cd22746     1 SLRVVPVKGDGRCLFRAVarglalatgGRPLSERRERADADALRKAVVEEIRKRRDELFE-------GSLVIEGDFDAYC 73

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1034660683 116 EDIVNTAAWGGQLELRALSHILQTPIE--IIQADSP---PIIV-GEEYSKKPLIlvymRHAYGLGEHY 177
Cdd:cd22746    74 QRMSHPDTWGGEPELLMLADVLQRPIAvyLPTPGKGglrKIQEyGEEYLGGEPI----RLLYNGGNHY 137
OTU_ALG13 cd22795
OTU (ovarian tumor) domain of bifunctional UDP-N-acetylglucosamine transferase and ...
 41-180 2.21e-05

OTU (ovarian tumor) domain of bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13; Bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 is also called asparagine-linked glycosylation 13 homolog, glycosyltransferase 28 domain-containing protein 1 (GLT28D1), or UDP-N-acetylglucosamine transferase subunit ALG13 homolog. It displays both glycosyltransferase (EC 2.4.1.141) and deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) activities. With ALG14, it forms a UDP-N-acetylglucosamine transferase that catalyzes the second step of eukaryotic N-linked glycosylation in the endoplasmic reticulum. ALG13 variants cause a form of early infantile epileptic encephalopathy known as EIEE36 refractory seizures, neurodevelopmental impairment, and poor prognosis; given the essential role of ALG13 in glycosylation, it is also considered a congenital disorder of glycosylation (CDG). ALG13 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438616 [Multi-domain]  Cd Length: 130  Bit Score: 42.49  E-value: 2.21e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  41 LAARQLEIKQIPSDGHCMYKAIEDQLKekDCALTVVALRSQTAEYMQSHVEDFLPFLTNPntgdmytpeeFQKYCEDIVN 120
Cdd:cd22795     5 LGSLGLYRKLTAKDASCLFRAVSEQLF--LCQIHHLEIRKACVSYMRANQCNFESYVEGS----------FEKYLERLED 72

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1034660683 121 TAAWGGQLELRALSHILQTPIEIIQ-ADSPPIIVGEEYSKKPLILVYMRHAyglgeHYNSV 180
Cdd:cd22795    73 PKESAGQLEISALSLIYNRDFILYRyPGKPPTYATDNGFEDKILLCCSSNG-----HYDSV 128
OTU_OTU1 cd22745
OTU (ovarian tumor) domain of ubiquitin thioesterase OTU1 and similar proteins; Ubiquitin ...
 46-182 3.28e-05

OTU (ovarian tumor) domain of ubiquitin thioesterase OTU1 and similar proteins; Ubiquitin thioesterase (EC 3.4.19.12) OTU1 is also called OTU domain-containing protein 1 in yeast, while human OTU1 is also called HIV-1-induced protease 7 (HIN7), DUBA-8, or OTU domain-containing protein 2 (OTUD2). OTU1 is a deubiquitinase (DUB) that catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU1 has been implicated in the ER-associated degradation (ERAD) pathway. In yeast, it counteracts the activity of Ufd2 by deubiquitinating Ufd2 substrates; Ufd2 is a E4 ubiquitin ligase that interacts with Cdc48, an AAA ATPase that plays a central role in the ERAD pathway by chaperoning proteins to the proteasome for destruction. OTU1 also functions as a substrate-processing factor of valosin-containing protein (VCP, the mammalian counterpart of yeast Cdc48) that is required for the retrotranslocation of the ERAD pathway. OTU1 has been shown to preferentially hydrolyze polyubiquitin chains with Lys48 linkages. It contains ubiquitin-like (Ubl) domain with a beta-grasp Ubl fold, a C2H2-type zinc finger, and an OTU (ovarian tumor) domain. This model represents the OTU domain that interacts with ubiquitin and possesses catalytic activity. OTU1 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. This family also contains plant OTU1 and OTU2.


Pssm-ID: 438582  Cd Length: 161  Bit Score: 42.09  E-value: 3.28e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  46 LEIKQIPSDGHCMYKAIeDQLKEKDCALTVVALRSQTAEYMQSHVEDF-LPFLTNPNtgdmytpeefQKYCEDIVNTAAW 124
Cdd:cd22745     3 LVRRVVPDDNSCLFTSI-SYLLEGGLLDSAPELREIVADAILSDPDTYnEAILGKPP----------DEYCAWILKPDSW 71

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683 125 GGQLELRALSHILQTPIEIIQADSPPIIV-GEE-YSKKPLILVYMrhayglGEHYNSVTR 182
Cdd:cd22745    72 GGAIELSILSKHFGVEICVVDVQTGRVDRfGEDkGYSKRIFLLYS------GIHYDALAL 125
Otubain_C65 cd22749
Otubain subfamily of ubiquitin thioesterases; The otubain subfamily is composed of otubain-1 ...
 74-178 4.18e-05

Otubain subfamily of ubiquitin thioesterases; The otubain subfamily is composed of otubain-1 (also called ubiquitin thioesterase OTUB1 or OTU domain-containing ubiquitin aldehyde-binding protein 1), otubain-2 (also called ubiquitin thioesterase OTUB2 or OTU domain-containing ubiquitin aldehyde-binding protein 2), and similar proteins. They function as deubiquitylases (DUBs)/ubiquitin thioesterases (EC 3.4.19.12). OTUB1 can specifically remove 'Lys-48'-linked conjugated ubiquitin from protein substrates, while OTUB2 mediates the deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. The otubain subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. Members of this subfamily are classified as family C65 cysteine proteases by MEROPS.


Pssm-ID: 438586 [Multi-domain]  Cd Length: 232  Bit Score: 42.71  E-value: 4.18e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  74 TVVALRSQTAEYMQSHVEDFLPFLTNPNTgdmytpeeFQKYCEDIVN-TAAWGGQLELRALSHILQTPIEIIQADSPPII 152
Cdd:cd22749   130 IVVFLRLLTSAYLKTNADDYEPFLFEGMS--------VEEFCEREVEpMGKEADHLQITALANALGVPVRVEYLDRSAGG 201

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1034660683 153 VGEEY--------SKKPLILVYMRhayglgEHYN 178
Cdd:cd22749   202 EVNFHefppedsdSLPVITLLYRP------GHYD 229
OTU_plant_OTU3-like cd22759
OTU (ovarian tumor) domain of deubiquitinating enzyme OTU3 from plants and similar proteins; ...
 109-166 1.21e-04

OTU (ovarian tumor) domain of deubiquitinating enzyme OTU3 from plants and similar proteins; Deubiquitinating enzyme OTU3, also called OTU domain-containing protein 3, is a deubiquitinase (DUB) or ubiquitin thiolesterase (EC 3.4.19.12) that catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU3 may play an important regulatory role at the level of protein turnover by preventing degradation. OTU3 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438596  Cd Length: 159  Bit Score: 40.79  E-value: 1.21e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1034660683 109 EEFQKYCEDIVNTAAWGGQLELRALSHILQTPIEIIQADSP-----------PII-VGEEYSK--------KPLILVY 166
Cdd:cd22759    72 GSLDRYCRRIQRPDFWGGESELLVLSKMLKQPIIVYIPESEaknggwgsgfiPIQkYGEEFAKgtkgrkgrKPVRLLY 149
OTU_RNAP_L_virus cd21880
OTU (ovarian tumor) domain of viral RNA-directed RNA polymerase L; RNA-directed RNA polymerase ...
 47-149 4.89e-04

OTU (ovarian tumor) domain of viral RNA-directed RNA polymerase L; RNA-directed RNA polymerase L is also called protein L, large structural protein, replicase, transcriptase, or ubiquitin thioesterase. It displays RNA-directed RNA polymerase (EC 2.7.7.48), deubiquitinase (DUB)/ubiquitin thiolesterase (EC 3.4.19.12), and deISGylating activities. It is a viral homolog of ovarian tumor protease (vOTU) that has been implicated in the downregulation of type I interferon immune response by removing post-translational modifying proteins ubiquitin (Ub) and the Ub-like interferon-simulated gene 15 (ISG15) from host cellular proteins. The attachment of Ub and ISG15 to cellular proteins mediates important innate antiviral responses, and their removal inhibits these antiviral pathways. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438580  Cd Length: 148  Bit Score: 38.74  E-value: 4.89e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  47 EIKQIPSDGHCMYKAIEDQLKEKDcaLTVVALRSQTAEYMQSHVEDFLPFLTNPNTgdmytPEEfqkYCEDIVNTAAWGG 126
Cdd:cd21880    23 EIERVPGDGNCFFRSIAELLFDTE--DEWRLVKNTIESYARANWDECPEARLYYLS-----LEE---YLRDAMKDGYWGG 92

                          90       100
                  ....*....|....*....|...
gi 1034660683 127 QLELRALSHILQTPIEIIQADSP 149
Cdd:cd21880    93 SLEAEILSKALGITIIIWVVDDS 115
OTU_CeDUB-like cd22755
OTU (ovarian tumor) domain of Caenorhabditis elegans deubiquitylating enzyme with USP/UBP and ...
 46-181 5.56e-04

OTU (ovarian tumor) domain of Caenorhabditis elegans deubiquitylating enzyme with USP/UBP and OTU domains, and similar proteins; This subfamily is composed of mostly uncharacterized proteins containing an OTU domain, similar to Caenorhabditis elegans deubiquitylating enzyme with USP/UBP and OTU domains. OTU domain-containing proteins function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. They belong to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438592 [Multi-domain]  Cd Length: 132  Bit Score: 38.40  E-value: 5.56e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  46 LEIKQIPSDGHCMYKAIedqlkekdcALTV-------VALRSQTAEYMQSHVEDFLPFLTNPNTgdmyTPEE-FQKYCED 117
Cdd:cd22755     1 CKTIKIVGDGNCFFRAL---------SYAItgsekyhRKIRKAIVDFLEKNPDEFRNLLRSDYE----SVEEyLEKSRMR 67

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1034660683 118 IVNTaaWGGQLELRALSHILQTPIEIIQAD-------SPPIIVGEEYSKKPLIlvYMRHAYglGEHYNSVT 181
Cdd:cd22755    68 YDGT--WATDVEIFAAATLLGVDIYVYSKGgykwllySPRFKLGKRNGSREAI--YLKNTN--GNHFEPVV 132
OTU_P87_VP80-like cd22757
OTU (ovarian tumor) domain of nucleopolyhedrovirus P87/VP80 protein and similar proteins; The ...
 48-145 4.28e-03

OTU (ovarian tumor) domain of nucleopolyhedrovirus P87/VP80 protein and similar proteins; The VP80 protein is a capsid-associated structural protein that was first identified as P87 in Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV); its homologs are found only in NPV genomes. The Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) VP80 protein is essential for the formation of both budded virus (BV) and occlusion-derived virus (ODV). It has also been shown to interact with the virus-triggered, nuclear F-actin cytoskeleton. P87/VP80 contains an N-terminal OTU domain. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. They belong to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438594 [Multi-domain]  Cd Length: 128  Bit Score: 35.64  E-value: 4.28e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  48 IKQIPSDGHCMYKAI-----EDQLKEKDcaltvvaLRSQTAEYMQSHVEDFLPFLTNPNtGDMYTPEEfqKYCEDIVNTA 122
Cdd:cd22757     3 VIPIPGDGACLFRALsyllyGTQSRHLE-------VRKEVVDYVVNNWDEFSIYTHDSE-GNNYKSAE--EYRADMSKPG 72

                          90       100
                  ....*....|....*....|...
gi 1034660683 123 AWGGQLELRALSHILQTPIEIIQ 145
Cdd:cd22757    73 TYGTLCELVAAAELYPFHFEVYR 95
OTU_plant_OTU1_2-like cd22793
OTU (ovarian tumor) domain of deubiquitinating enzyme OTU1 and OTU2 from plants and similar ...
 51-134 8.44e-03

OTU (ovarian tumor) domain of deubiquitinating enzyme OTU1 and OTU2 from plants and similar proteins; Deubiquitinating enzyme OTU2, also called OTU domain-containing protein 2, is a deubiquitinase (DUB) or ubiquitin thiolesterase (EC 3.4.19.12) that catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU2 exhibited equivalent binding affinities for K48- and K63-linked ubiquitin chains and no cleavage activity toward linear UB chains. It may also be involved in endoplasmic-reticulum-associated protein degradation (ERAD). OTU2 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438614  Cd Length: 163  Bit Score: 35.38  E-value: 8.44e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034660683  51 IPSDGHCMYKAI----EDQLKEKDCALTVVA--LRSQTAEYMQShvedflpFLTNPNTGdmytpeefqkYCEDIVNTAAW 124
Cdd:cd22793     8 IDSDNSCLFNAVgyvmEGSRKKAPELRQVIAdaVLSDPFEYNEA-------FLGKSNKE----------YCEWILNPNSW 70

                          90
                  ....*....|
gi 1034660683 125 GGQLELRALS 134
Cdd:cd22793    71 GGAIELSILS 80
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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