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Conserved domains on  [gi|755503406|ref|XP_011238329|]
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rho guanine nucleotide exchange factor 2 isoform X4 [Mus musculus]

Protein Classification

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PH_ARHGEF2 cd13393
Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain; ARHGEF2, also called ...
671-786 2.47e-73

Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain; ARHGEF2, also called GEF-H1, acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. It is thought to play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. ARHGEF2 contains a C1 domain followed by Dbl-homology (DH) and pleckstrin-homology (PH) domains which bind and catalyze the exchange of GDP for GTP on RhoA. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


:

Pssm-ID: 275428  Cd Length: 116  Bit Score: 238.24  E-value: 2.47e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  671 RRKLIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLDKPSVVSLQNLIVRDIANQAKGMFLISSGPPEMY 750
Cdd:cd13393     1 RRKLIHDGCLLWKTASGRFKDVQVLLMTDVLVFLQEKDQKYIFPTLDKPAVISLQNLIVRDIANQEKGMFLISAAPPEMY 80
                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 755503406  751 EVHAASRDDRTTWIRVIQQSVRLCPSREDFPLIETE 786
Cdd:cd13393    81 EVHAASRDDRNTWMRLIQQTVKTCPSREEFPLIETE 116
RhoGEF cd00160
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous ...
437-631 9.75e-44

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.


:

Pssm-ID: 238091 [Multi-domain]  Cd Length: 181  Bit Score: 156.69  E-value: 9.75e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  437 KQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELQ-MEPEVVQGLFPCVDELSDIHTRFLNQLLERRRQALCPGstrnfv 515
Cdd:cd00160     1 RQEVIKELLQTERNYVRDLKLLVEVFLKPLDKELLpLSPEEVELLFGNIEEIYEFHRIFLKSLEERVEEWDKSG------ 74
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  516 iHRLGDLLISQFSgsnaeqMRKTYSEFCSRHTKALKLYKELYARDKRFQQFIRKmtRSAVLKRHGVQECILLVTQRITKY 595
Cdd:cd00160    75 -PRIGDVFLKLAP------FFKIYSEYCSNHPDALELLKKLKKFNKFFQEFLEK--AESECGRLKLESLLLKPVQRLTKY 145
                         170       180       190
                  ....*....|....*....|....*....|....*.
gi 755503406  596 PVLINRILQNSHGVEEEYQDLASALGLVKELLSNVD 631
Cdd:cd00160   146 PLLLKELLKHTPDGHEDREDLKKALEAIKEVASQVN 181
C1_ARHGEF2 cd20877
protein kinase C conserved region 1 (C1 domain) found in Rho guanine nucleotide exchange ...
235-295 3.87e-39

protein kinase C conserved region 1 (C1 domain) found in Rho guanine nucleotide exchange factor 2 (ARHGEF2) and similar proteins; ARHGEF2, also called guanine nucleotide exchange factor H1 (GEF-H1), microtubule-regulated Rho-GEF, or proliferating cell nucleolar antigen p40, acts as guanine nucleotide exchange factor (GEF) that activates Rho-GTPases by promoting the exchange of GDP for GTP. It is thought to play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. ARHGEF2 may be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. It contains a C1 domain followed by Dbl-homology (DH) and pleckstrin-homology (PH) domains which bind and catalyze the exchange of GDP for GTP on RhoA. This model corresponds to the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


:

Pssm-ID: 410427  Cd Length: 61  Bit Score: 139.33  E-value: 3.87e-39
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 755503406  235 RYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQK 295
Cdd:cd20877     1 RYTNGHLFTTITVSGTTMCSACNKSITAKEALICPTCNVTIHNRCKDTLPNCTKVKQKQQK 61
COG4913 super family cl25907
Uncharacterized conserved protein, contains a C-terminal ATPase domain [Function unknown];
941-1086 5.19e-06

Uncharacterized conserved protein, contains a C-terminal ATPase domain [Function unknown];


The actual alignment was detected with superfamily member COG4913:

Pssm-ID: 443941 [Multi-domain]  Cd Length: 1089  Bit Score: 50.68  E-value: 5.19e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  941 LINLYGLLHGLQAVVVQQERLMEALFPEgPERWEKLsRANSRDGEAGRAAVASVTPEKQATELALLQRQHTLLQEELRRc 1020
Cdd:COG4913   230 LVEHFDDLERAHEALEDAREQIELLEPI-RELAERY-AAARERLAELEYLRAALRLWFAQRRLELLEAELEELRAELAR- 306
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 755503406 1021 qrlgeerateagsLEARLRESEQARALLEREAEEIRRQLAALGQN--EPLPAEAPWARRPLDPRRRSL 1086
Cdd:COG4913   307 -------------LEAELERLEARLDALREELDELEAQIRGNGGDrlEQLEREIERLERELEERERRR 361
 
Name Accession Description Interval E-value
PH_ARHGEF2 cd13393
Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain; ARHGEF2, also called ...
671-786 2.47e-73

Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain; ARHGEF2, also called GEF-H1, acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. It is thought to play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. ARHGEF2 contains a C1 domain followed by Dbl-homology (DH) and pleckstrin-homology (PH) domains which bind and catalyze the exchange of GDP for GTP on RhoA. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275428  Cd Length: 116  Bit Score: 238.24  E-value: 2.47e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  671 RRKLIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLDKPSVVSLQNLIVRDIANQAKGMFLISSGPPEMY 750
Cdd:cd13393     1 RRKLIHDGCLLWKTASGRFKDVQVLLMTDVLVFLQEKDQKYIFPTLDKPAVISLQNLIVRDIANQEKGMFLISAAPPEMY 80
                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 755503406  751 EVHAASRDDRTTWIRVIQQSVRLCPSREDFPLIETE 786
Cdd:cd13393    81 EVHAASRDDRNTWMRLIQQTVKTCPSREEFPLIETE 116
RhoGEF cd00160
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous ...
437-631 9.75e-44

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 238091 [Multi-domain]  Cd Length: 181  Bit Score: 156.69  E-value: 9.75e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  437 KQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELQ-MEPEVVQGLFPCVDELSDIHTRFLNQLLERRRQALCPGstrnfv 515
Cdd:cd00160     1 RQEVIKELLQTERNYVRDLKLLVEVFLKPLDKELLpLSPEEVELLFGNIEEIYEFHRIFLKSLEERVEEWDKSG------ 74
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  516 iHRLGDLLISQFSgsnaeqMRKTYSEFCSRHTKALKLYKELYARDKRFQQFIRKmtRSAVLKRHGVQECILLVTQRITKY 595
Cdd:cd00160    75 -PRIGDVFLKLAP------FFKIYSEYCSNHPDALELLKKLKKFNKFFQEFLEK--AESECGRLKLESLLLKPVQRLTKY 145
                         170       180       190
                  ....*....|....*....|....*....|....*.
gi 755503406  596 PVLINRILQNSHGVEEEYQDLASALGLVKELLSNVD 631
Cdd:cd00160   146 PLLLKELLKHTPDGHEDREDLKKALEAIKEVASQVN 181
RhoGEF smart00325
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange ...
440-632 2.23e-41

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains. Improved coverage.


Pssm-ID: 214619 [Multi-domain]  Cd Length: 180  Bit Score: 150.14  E-value: 2.23e-41
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406    440 VIYELIQTELHHVRTLKIMTRLFRTGMLEELQ-MEPEVVQGLFPCVDELSDIHTRFLNQLLERRRQALCPGstrnfviHR 518
Cdd:smart00325    1 VLKELLQTERNYVRDLKLLVEVFLKPLKKELKlLSPNELETLFGNIEEIYEFHRDFLDELEERIEEWDDSV-------ER 73
                            90       100       110       120       130       140       150       160
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406    519 LGDLLIsqfsgsNAEQMRKTYSEFCSRHTKALKLYKELyARDKRFQQFIRKMTRSAVLKRHGVQECILLVTQRITKYPVL 598
Cdd:smart00325   74 IGDVFL------KLEEFFKIYSEYCSNHPDALELLKKL-KKNPRFQKFLKEIESSPQCRRLTLESLLLKPVQRLTKYPLL 146
                           170       180       190
                    ....*....|....*....|....*....|....
gi 755503406    599 INRILQNSHGVEEEYQDLASALGLVKELLSNVDQ 632
Cdd:smart00325  147 LKELLKHTPEDHEDREDLKKALKAIKELANQVNE 180
C1_ARHGEF2 cd20877
protein kinase C conserved region 1 (C1 domain) found in Rho guanine nucleotide exchange ...
235-295 3.87e-39

protein kinase C conserved region 1 (C1 domain) found in Rho guanine nucleotide exchange factor 2 (ARHGEF2) and similar proteins; ARHGEF2, also called guanine nucleotide exchange factor H1 (GEF-H1), microtubule-regulated Rho-GEF, or proliferating cell nucleolar antigen p40, acts as guanine nucleotide exchange factor (GEF) that activates Rho-GTPases by promoting the exchange of GDP for GTP. It is thought to play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. ARHGEF2 may be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. It contains a C1 domain followed by Dbl-homology (DH) and pleckstrin-homology (PH) domains which bind and catalyze the exchange of GDP for GTP on RhoA. This model corresponds to the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410427  Cd Length: 61  Bit Score: 139.33  E-value: 3.87e-39
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 755503406  235 RYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQK 295
Cdd:cd20877     1 RYTNGHLFTTITVSGTTMCSACNKSITAKEALICPTCNVTIHNRCKDTLPNCTKVKQKQQK 61
PH_16 pfam17838
PH domain;
664-772 6.63e-37

PH domain;


Pssm-ID: 436083  Cd Length: 127  Bit Score: 135.22  E-value: 6.63e-37
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406   664 FGRDELLRRKLIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLDKPS----------VVSLQNLIVRDIA 733
Cdd:pfam17838    7 FKKLDLTTRKLIHEGPLTWRNSKGKLVEVHALLLEDILVLLQEKDQKLVLACLSTGSenvdqktqspIISLKKLIVREVA 86
                           90       100       110       120
                   ....*....|....*....|....*....|....*....|.
gi 755503406   734 NQAKGMFLISSGP--PEMYEVHAASRDDRTTWIRVIQQSVR 772
Cdd:pfam17838   87 TDKKAFFLISTSPsdPQMYELHASTKSERNTWTKLIQDAIE 127
RhoGEF pfam00621
RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called ...
440-631 1.78e-34

RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that pfam00169 domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 459876 [Multi-domain]  Cd Length: 176  Bit Score: 130.11  E-value: 1.78e-34
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406   440 VIYELIQTELHHVRTLKIMTRLFRTGMLEELQMEPEVVQGLFPCVDELSDIHTRFLnqlLERRRQAlcpgstrNFVIHRL 519
Cdd:pfam00621    1 VIKELLQTERSYVRDLEILVEVFLPPNSKPLSESEEEIKTIFSNIEEIYELHRQLL---LEELLKE-------WISIQRI 70
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406   520 GDLLISQFSGsnaeqmRKTYSEFCSRHTKALKLYKELYARDKRFQQFIRKMTRSAVLKRHGVQECILLVTQRITKYPVLI 599
Cdd:pfam00621   71 GDIFLKFAPG------FKVYSTYCSNYPKALKLLKKLLKKNPKFRAFLEELEANPECRGLDLNSFLIKPVQRIPRYPLLL 144
                          170       180       190
                   ....*....|....*....|....*....|..
gi 755503406   600 NRILQNSHGVEEEYQDLASALGLVKELLSNVD 631
Cdd:pfam00621  145 KELLKHTPPDHPDYEDLKKALEAIKEVAKQIN 176
PH smart00233
Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The ...
674-772 4.07e-10

Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The domain family possesses multiple functions including the abilities to bind inositol phosphates, and various proteins. PH domains have been found to possess inserted domains (such as in PLC gamma, syntrophins) and to be inserted within other domains. Mutations in Brutons tyrosine kinase (Btk) within its PH domain cause X-linked agammaglobulinaemia (XLA) in patients. Point mutations cluster into the positively charged end of the molecule around the predicted binding site for phosphatidylinositol lipids.


Pssm-ID: 214574 [Multi-domain]  Cd Length: 102  Bit Score: 57.94  E-value: 4.07e-10
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406    674 LIHEGCLLWKTATGR--FKDVLLLLMTDVLVFLQEKDQKYIFTsldKPSVVSLQNLIVRDIANQ----AKGMFLISSGPP 747
Cdd:smart00233    1 VIKEGWLYKKSGGGKksWKKRYFVLFNSTLLYYKSKKDKKSYK---PKGSIDLSGCTVREAPDPdsskKPHCFEIKTSDR 77
                            90       100
                    ....*....|....*....|....*
gi 755503406    748 EMYEVHAASRDDRTTWIRVIQQSVR 772
Cdd:smart00233   78 KTLLLQAESEEEREKWVEALRKAIA 102
C1 smart00109
Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains); Some bind phorbol ...
240-286 1.82e-08

Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains); Some bind phorbol esters and diacylglycerol. Some bind RasGTP. Zinc-binding domains.


Pssm-ID: 197519  Cd Length: 50  Bit Score: 51.31  E-value: 1.82e-08
                            10        20        30        40        50
                    ....*....|....*....|....*....|....*....|....*....|
gi 755503406    240 HLFTTISVSGMTMCYACNKSI--TAKEALICPTCNVTIHNRCKDTL-ANC 286
Cdd:smart00109    1 HKHVFRTFTKPTFCCVCRKSIwgSFKQGLRCSECKVKCHKKCADKVpKAC 50
ROM1 COG5422
RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction ...
409-709 6.36e-08

RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction mechanisms];


Pssm-ID: 227709 [Multi-domain]  Cd Length: 1175  Bit Score: 57.21  E-value: 6.36e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  409 DEKDFEADSWSLAVDSSFLQQHKKEVMKKQDVIYELIQTELHHVRTLKImTRLFRTGMLEELQMEPE-----VVQGLFPC 483
Cdd:COG5422   457 DKFDEEKNLWTLSVPKEVWESLPKQEIKRQEAIYEVIYTERDFVKDLEY-LRDTWIKPLEESNIIPEnarrnFIKHVFAN 535
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  484 VDELSDIHTRFLNQLleRRRQALCPgstrnfVIHRLGDLLIsqfsgsnaeqmrktysEFCSRHTKALKLYKE-LYAR--- 559
Cdd:COG5422   536 INEIYAVNSKLLKAL--TNRQCLSP------IVNGIADIFL----------------DYVPKFEPFIKYGASqPYAKyef 591
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  560 ------DKRFQQFIRKMTRSAVLKRHGVQECILLVTQRITKYPVLINRILQNSHGVEEEYQDLASALGLVKELLSNVDQD 633
Cdd:COG5422   592 ereksvNPNFARFDHEVERLDESRKLELDGYLTKPTTRLARYPLLLEEVLKFTDPDNPDTEDIPKVIDMLREFLSRLNFE 671
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  634 VHELEKEARL----QEIYNRMDPRAQTPvpgkgpfgRDEllRRKLIHEGCL----LWKTATGRFKDVLLLLMTDVLVFLQ 705
Cdd:COG5422   672 SGKAENRGDLfhlnQQLLFKPEYVNLGL--------NDE--YRKIIFKGVLkrkaKSKTDGSLRGDIQFFLLDNMLLFCK 741

                  ....
gi 755503406  706 EKDQ 709
Cdd:COG5422   742 AKAV 745
COG4913 COG4913
Uncharacterized conserved protein, contains a C-terminal ATPase domain [Function unknown];
941-1086 5.19e-06

Uncharacterized conserved protein, contains a C-terminal ATPase domain [Function unknown];


Pssm-ID: 443941 [Multi-domain]  Cd Length: 1089  Bit Score: 50.68  E-value: 5.19e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  941 LINLYGLLHGLQAVVVQQERLMEALFPEgPERWEKLsRANSRDGEAGRAAVASVTPEKQATELALLQRQHTLLQEELRRc 1020
Cdd:COG4913   230 LVEHFDDLERAHEALEDAREQIELLEPI-RELAERY-AAARERLAELEYLRAALRLWFAQRRLELLEAELEELRAELAR- 306
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 755503406 1021 qrlgeerateagsLEARLRESEQARALLEREAEEIRRQLAALGQN--EPLPAEAPWARRPLDPRRRSL 1086
Cdd:COG4913   307 -------------LEAELERLEARLDALREELDELEAQIRGNGGDrlEQLEREIERLERELEERERRR 361
 
Name Accession Description Interval E-value
PH_ARHGEF2 cd13393
Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain; ARHGEF2, also called ...
671-786 2.47e-73

Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain; ARHGEF2, also called GEF-H1, acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. It is thought to play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. ARHGEF2 contains a C1 domain followed by Dbl-homology (DH) and pleckstrin-homology (PH) domains which bind and catalyze the exchange of GDP for GTP on RhoA. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275428  Cd Length: 116  Bit Score: 238.24  E-value: 2.47e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  671 RRKLIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLDKPSVVSLQNLIVRDIANQAKGMFLISSGPPEMY 750
Cdd:cd13393     1 RRKLIHDGCLLWKTASGRFKDVQVLLMTDVLVFLQEKDQKYIFPTLDKPAVISLQNLIVRDIANQEKGMFLISAAPPEMY 80
                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 755503406  751 EVHAASRDDRTTWIRVIQQSVRLCPSREDFPLIETE 786
Cdd:cd13393    81 EVHAASRDDRNTWMRLIQQTVKTCPSREEFPLIETE 116
PH_ARHGEF18 cd15794
Rho guanine nucleotide exchange factor 18 Pleckstrin homology (PH) domain; ARHGEF18, also ...
671-786 2.77e-44

Rho guanine nucleotide exchange factor 18 Pleckstrin homology (PH) domain; ARHGEF18, also called p114RhoGEF, is a key regulator of RhoA-Rock2 signaling that is crucial for maintenance of polarity in the vertebrate retinal epithelium, and consequently is essential for cellular differentiation, morphology and eventually organ function. ARHGEF18 contains Dbl-homology (DH) and pleckstrin-homology (PH) domains which bind and catalyze the exchange of GDP for GTP on RhoA. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275437  Cd Length: 119  Bit Score: 155.83  E-value: 2.77e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  671 RRKLIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLD-KPSVVSLQNLIVRDIANQAKGMFLI--SSGPP 747
Cdd:cd15794     1 RRQLLLEGMLYWKAASGRLKDILALLLTDVLLLLQEKDQKYVFASVDsKPPVISLQKLIVREVANEEKAMFLIsaSLNGP 80
                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 755503406  748 EMYEVHAASRDDRTTWIRVIQQSVRLCPSREDFPLIETE 786
Cdd:cd15794    81 EMYEIHTNSKEDRNTWMAHIRRAVESCPDEEEGLFSEPE 119
PH_p190RhoGEF cd14680
Rho guanine nucleotide exchange factor Pleckstrin homology domain; p190RhoGEF (also called ...
674-771 4.09e-44

Rho guanine nucleotide exchange factor Pleckstrin homology domain; p190RhoGEF (also called RIP2 or ARHGEF28) belongs to regulator of G-protein signaling (RGS) domain-containing RhoGEFs that are RhoA-selective and directly activated by the Galpha12/13 family of heterotrimeric G proteins. In addition to the Dbl homology (DH)-PH domain, p190RhoGEF contains an N-terminal C1 (Protein kinase C conserved region 1) domain. The DH-PH domains bind and catalyze the exchange of GDP for GTP on RhoA. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275430  Cd Length: 101  Bit Score: 154.77  E-value: 4.09e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  674 LIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLD-KPSVVSLQNLIVRDIANQAKGMFLI--SSGPPEMY 750
Cdd:cd14680     1 LLHEGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDqKPPVICLQKLIVREVANEERGMFLIsaSSAGPEMY 80
                          90       100
                  ....*....|....*....|.
gi 755503406  751 EVHAASRDDRTTWIRVIQQSV 771
Cdd:cd14680    81 EIHTSSKEERNNWMRLIQEAV 101
RhoGEF cd00160
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous ...
437-631 9.75e-44

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 238091 [Multi-domain]  Cd Length: 181  Bit Score: 156.69  E-value: 9.75e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  437 KQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELQ-MEPEVVQGLFPCVDELSDIHTRFLNQLLERRRQALCPGstrnfv 515
Cdd:cd00160     1 RQEVIKELLQTERNYVRDLKLLVEVFLKPLDKELLpLSPEEVELLFGNIEEIYEFHRIFLKSLEERVEEWDKSG------ 74
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  516 iHRLGDLLISQFSgsnaeqMRKTYSEFCSRHTKALKLYKELYARDKRFQQFIRKmtRSAVLKRHGVQECILLVTQRITKY 595
Cdd:cd00160    75 -PRIGDVFLKLAP------FFKIYSEYCSNHPDALELLKKLKKFNKFFQEFLEK--AESECGRLKLESLLLKPVQRLTKY 145
                         170       180       190
                  ....*....|....*....|....*....|....*.
gi 755503406  596 PVLINRILQNSHGVEEEYQDLASALGLVKELLSNVD 631
Cdd:cd00160   146 PLLLKELLKHTPDGHEDREDLKKALEAIKEVASQVN 181
PH_ARHGEF2_18_like cd15789
rho guanine nucleotide exchange factor; RhoGEFs belongs to regulator of G-protein signaling ...
674-771 3.84e-43

rho guanine nucleotide exchange factor; RhoGEFs belongs to regulator of G-protein signaling (RGS) domain-containing RhoGEFs that are RhoA-selective and directly activated by the Galpha12/13 family of heterotrimeric G proteins. The members here all contain Dbl homology (DH)-PH domains. In addition some members contain N-terminal C1 (Protein kinase C conserved region 1) domains, PDZ (also called DHR/Dlg homologous regions) domains, ANK (ankyrin) domains, and RGS (Regulator of G-protein signalling) domains or C-terminal ATP-synthase B subunit. The DH-PH domains bind and catalyze the exchange of GDP for GTP on RhoA. RhoGEF2/Rho guanine nucleotide exchange factor 2, p114RhoGEF/p114 Rho guanine nucleotide exchange factor, p115RhoGEF, p190RhoGEF, PRG/PDZ Rho guanine nucleotide exchange factor, RhoGEF 11, RhoGEF 12, RhoGEF 18, AKAP13/A-kinase anchoring protein 13, and LARG/Leukemia-associated Rho guanine nucleotide exchange factor are included in this CD. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275432  Cd Length: 102  Bit Score: 152.23  E-value: 3.84e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  674 LIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLD-KPSVVSLQNLIVRDIANQAKGMFLISSGPPEMYEV 752
Cdd:cd15789     1 LKFEGTAWLKQARGKTKDVLVVVLTDVLFFLQEKDQKYVFVSPDnKAGVVSLQKLLVREKAGQEKRMFLISASPDGMPEM 80
                          90       100
                  ....*....|....*....|..
gi 755503406  753 HAASRD---DRTTWIRVIQQSV 771
Cdd:cd15789    81 YELKVQkpkDKNTWIQTIRQAV 102
RhoGEF smart00325
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange ...
440-632 2.23e-41

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains. Improved coverage.


Pssm-ID: 214619 [Multi-domain]  Cd Length: 180  Bit Score: 150.14  E-value: 2.23e-41
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406    440 VIYELIQTELHHVRTLKIMTRLFRTGMLEELQ-MEPEVVQGLFPCVDELSDIHTRFLNQLLERRRQALCPGstrnfviHR 518
Cdd:smart00325    1 VLKELLQTERNYVRDLKLLVEVFLKPLKKELKlLSPNELETLFGNIEEIYEFHRDFLDELEERIEEWDDSV-------ER 73
                            90       100       110       120       130       140       150       160
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406    519 LGDLLIsqfsgsNAEQMRKTYSEFCSRHTKALKLYKELyARDKRFQQFIRKMTRSAVLKRHGVQECILLVTQRITKYPVL 598
Cdd:smart00325   74 IGDVFL------KLEEFFKIYSEYCSNHPDALELLKKL-KKNPRFQKFLKEIESSPQCRRLTLESLLLKPVQRLTKYPLL 146
                           170       180       190
                    ....*....|....*....|....*....|....
gi 755503406    599 INRILQNSHGVEEEYQDLASALGLVKELLSNVDQ 632
Cdd:smart00325  147 LKELLKHTPEDHEDREDLKKALKAIKELANQVNE 180
C1_ARHGEF2 cd20877
protein kinase C conserved region 1 (C1 domain) found in Rho guanine nucleotide exchange ...
235-295 3.87e-39

protein kinase C conserved region 1 (C1 domain) found in Rho guanine nucleotide exchange factor 2 (ARHGEF2) and similar proteins; ARHGEF2, also called guanine nucleotide exchange factor H1 (GEF-H1), microtubule-regulated Rho-GEF, or proliferating cell nucleolar antigen p40, acts as guanine nucleotide exchange factor (GEF) that activates Rho-GTPases by promoting the exchange of GDP for GTP. It is thought to play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. ARHGEF2 may be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. It contains a C1 domain followed by Dbl-homology (DH) and pleckstrin-homology (PH) domains which bind and catalyze the exchange of GDP for GTP on RhoA. This model corresponds to the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410427  Cd Length: 61  Bit Score: 139.33  E-value: 3.87e-39
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 755503406  235 RYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQK 295
Cdd:cd20877     1 RYTNGHLFTTITVSGTTMCSACNKSITAKEALICPTCNVTIHNRCKDTLPNCTKVKQKQQK 61
PH_AKAP13 cd13392
A-kinase anchoring protein 13 Pleckstrin homology (PH) domain; The Rho-specific GEF activity ...
674-771 4.08e-37

A-kinase anchoring protein 13 Pleckstrin homology (PH) domain; The Rho-specific GEF activity of AKAP13 (also called Brx-1, AKAP-Lbc, and proto-Lbc) mediates signaling downstream of G-protein coupled receptors and Toll-like receptor 2. It plays a role in cell growth, cell development and actin fiber formation. Protein kinase A (PKA) binds and phosphorylates AKAP13, regulating its Rho-GEF activity. Alternative splicing of this gene in humans has at least 3 transcript variants encoding different isoforms (i.e. proto-/onco-Lymphoid blast crisis, Lbc and breast cancer nuclear receptor-binding auxiliary protein, Brx) containing a dbl oncogene homology (DH) domain and PH domain which are required for full transforming activity. The DH domain is associated with guanine nucleotide exchange activation while the PH domain has multiple functions including determine protein sub-cellular localisation via phosphoinositide interactions, while others bind protein partners. Other ligands include protein kinase C which is bound by the PH domain of AKAP13, serving to activate protein kinase D and mobilize a cardiac hypertrophy signaling pathway. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275427  Cd Length: 103  Bit Score: 135.04  E-value: 4.08e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  674 LIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLD-KPSVVSLQNLIVRDIANQAKGMFLISSG--PPEMY 750
Cdd:cd13392     1 LVRDGPVSLKNTAGRLKEVQAVLLSDVLVFLQEKDQKYVFASLDqKSTVISLKKLIVREVAHEEKGLFLISMGiaDPEMV 80
                          90       100
                  ....*....|....*....|.
gi 755503406  751 EVHAASRDDRTTWIRVIQQSV 771
Cdd:cd13392    81 EVHASSKEERNSWMQIIQDTI 101
PH_16 pfam17838
PH domain;
664-772 6.63e-37

PH domain;


Pssm-ID: 436083  Cd Length: 127  Bit Score: 135.22  E-value: 6.63e-37
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406   664 FGRDELLRRKLIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLDKPS----------VVSLQNLIVRDIA 733
Cdd:pfam17838    7 FKKLDLTTRKLIHEGPLTWRNSKGKLVEVHALLLEDILVLLQEKDQKLVLACLSTGSenvdqktqspIISLKKLIVREVA 86
                           90       100       110       120
                   ....*....|....*....|....*....|....*....|.
gi 755503406   734 NQAKGMFLISSGP--PEMYEVHAASRDDRTTWIRVIQQSVR 772
Cdd:pfam17838   87 TDKKAFFLISTSPsdPQMYELHASTKSERNTWTKLIQDAIE 127
PH_RhoGEF cd13329
Rho guanine nucleotide exchange factor Pleckstrin homology domain; RhoGEFs belongs to ...
674-771 1.55e-34

Rho guanine nucleotide exchange factor Pleckstrin homology domain; RhoGEFs belongs to regulator of G-protein signaling (RGS) domain-containing RhoGEFs that are RhoA-selective and directly activated by the Galpha12/13 family of heterotrimeric G proteins. The members here all contain Dbl homology (DH)-PH domains. In addition some members contain N-terminal C1 (Protein kinase C conserved region 1) domains, PDZ (also called DHR/Dlg homologous regions) domains, ANK (ankyrin) domains, and RGS (Regulator of G-protein signalling) domains or C-terminal ATP-synthase B subunit. The DH-PH domains bind and catalyze the exchange of GDP for GTP on RhoA. RhoGEF2/Rho guanine nucleotide exchange factor 2, p114RhoGEF/p114 Rho guanine nucleotide exchange factor, p115RhoGEF, p190RhoGEF, PRG/PDZ Rho guanine nucleotide exchange factor, RhoGEF 11, RhoGEF 12, RhoGEF 18, AKAP13/A-kinase anchoring protein 13, and LARG/Leukemia-associated Rho guanine nucleotide exchange factor are included in this CD. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275411  Cd Length: 109  Bit Score: 127.76  E-value: 1.55e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  674 LIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYI--------FTSLD-KPSVVSLQNLIVRDIANQAKGMFLI-- 742
Cdd:cd13329     1 LIHEGPLTWKVARGKLIEVHVLLLEDLLVLLQKQDDKYLlklhltgsFDSKDtKSPVIKLSTLLVREVATDKKAFFLIst 80
                          90       100
                  ....*....|....*....|....*....
gi 755503406  743 SSGPPEMYEVHAASRDDRTTWIRVIQQSV 771
Cdd:cd13329    81 SKNGPQMYELVANSSSERKTWIKHISDAV 109
RhoGEF pfam00621
RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called ...
440-631 1.78e-34

RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that pfam00169 domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 459876 [Multi-domain]  Cd Length: 176  Bit Score: 130.11  E-value: 1.78e-34
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406   440 VIYELIQTELHHVRTLKIMTRLFRTGMLEELQMEPEVVQGLFPCVDELSDIHTRFLnqlLERRRQAlcpgstrNFVIHRL 519
Cdd:pfam00621    1 VIKELLQTERSYVRDLEILVEVFLPPNSKPLSESEEEIKTIFSNIEEIYELHRQLL---LEELLKE-------WISIQRI 70
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406   520 GDLLISQFSGsnaeqmRKTYSEFCSRHTKALKLYKELYARDKRFQQFIRKMTRSAVLKRHGVQECILLVTQRITKYPVLI 599
Cdd:pfam00621   71 GDIFLKFAPG------FKVYSTYCSNYPKALKLLKKLLKKNPKFRAFLEELEANPECRGLDLNSFLIKPVQRIPRYPLLL 144
                          170       180       190
                   ....*....|....*....|....*....|..
gi 755503406   600 NRILQNSHGVEEEYQDLASALGLVKELLSNVD 631
Cdd:pfam00621  145 KELLKHTPPDHPDYEDLKKALEAIKEVAKQIN 176
C1_p190RhoGEF-like cd20815
protein kinase C conserved region 1 (C1 domain) found in the 190 kDa guanine nucleotide ...
237-289 2.88e-20

protein kinase C conserved region 1 (C1 domain) found in the 190 kDa guanine nucleotide exchange factor (p190RhoGEF)-like family; The p190RhoGEF-like protein family includes p190RhoGEF, Rho guanine nucleotide exchange factor 2 (ARHGEF2), A-kinase anchor protein 13 (AKAP-13) and similar proteins. p190RhoGEF is a brain-enriched, RhoA-specific guanine nucleotide exchange factor that regulates signaling pathways downstream of integrins and growth factor receptors. It is involved in axonal branching, synapse formation and dendritic morphogenesis, as well as in focal adhesion formation, cell motility and B-lymphocytes activation. ARHGEF2 acts as a guanine nucleotide exchange factor (GEF) that activates Rho-GTPases by promoting the exchange of GDP for GTP. It is thought to play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. AKAP-13 is a scaffold protein that plays an important role in assembling signaling complexes downstream of several types of G protein-coupled receptors. It activates RhoA in response to signaling via G protein-coupled receptors via its function as Rho guanine nucleotide exchange factor. It may also activate other Rho family members. AKAP-13 plays a role in cell growth, cell development and actin fiber formation. Members of this family share a common domain architecture containing C1, RhoGEF or Dbl-homologous (DH), and Pleckstrin Homology (PH) domains. Some members may contain additional domains such as the DUF5401 domain. This model describes the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410365  Cd Length: 54  Bit Score: 85.16  E-value: 2.88e-20
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 755503406  237 TNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIH-NRCKDTLANCTKV 289
Cdd:cd20815     1 KNTHQFVPVSFSNSTKCDVCSKPLTNKPALQCENCSVNVHdSSCKDQLADCTKF 54
C1_AKAP13 cd20878
protein kinase C conserved region 1 (C1 domain) found in A-kinase anchor protein 13 (AKAP-13) ...
233-292 2.89e-20

protein kinase C conserved region 1 (C1 domain) found in A-kinase anchor protein 13 (AKAP-13) and similar proteins; AKAP-13, also called AKAP-Lbc, breast cancer nuclear receptor-binding auxiliary protein (Brx-1), guanine nucleotide exchange factor Lbc, human thyroid-anchoring protein 31, lymphoid blast crisis oncogene (LBC oncogene), non-oncogenic Rho GTPase-specific GTP exchange factor, protein kinase A-anchoring protein 13 (PRKA13), or p47, is a scaffold protein that plays an important role in assembling signaling complexes downstream of several types of G protein-coupled receptors (GPCRs). It activates RhoA in response to GPCR signaling via its function as a Rho guanine nucleotide exchange factor. It may also activate other Rho family members. AKAP-13 plays a role in cell growth, cell development and actin fiber formation. Its Rho-GEF activity is regulated by protein kinase A (PKA), through binding and phosphorylation. Alternative splicing of this gene in humans has at least 3 transcript variants encoding different isoforms (i.e. proto-/onco-Lymphoid blast crisis, Lbc and breast cancer nuclear receptor-binding auxiliary protein, and Brx) that contain a C1 domain followed by a dbl oncogene homology (DH) domain and a PH domain which are required for full transforming activity. This model corresponds to the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410428  Cd Length: 60  Bit Score: 85.09  E-value: 2.89e-20
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  233 DARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQK 292
Cdd:cd20878     1 DKKTLNGHVFSPVSSVGPTQCYHCSKPLNTKDAFLCANCNVQVHKGCRESLPVCAKVKMK 60
PH_PRG cd13391
PDZ Rho guanine nucleotide exchange factor Pleckstrin homology (PH) domain; PRG (also called ...
649-772 1.09e-15

PDZ Rho guanine nucleotide exchange factor Pleckstrin homology (PH) domain; PRG (also called RhoGEF11) belongs to regulator of G-protein signaling (RGS) domain-containing RhoGEFs that are RhoA-selective and directly activated by the Galpha12/13 family of heterotrimeric G proteins. RhoGEFs activate Rho GTPases regulating cytoskeletal structure, gene transcription, and cell migration. PRG contains an N-terminal PDZ domain, a regulators of G-protein signaling-like (RGSL) domain, a linker region, and a C-terminal Dbl-homology (DH) and pleckstrin-homology (PH) domains which bind and catalyze the exchange of GDP for GTP on RhoA. As is the case in p115-RhoGEF, it is thought that the PRG activated by relieving autoinhibition caused by the linker region. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275426  Cd Length: 142  Bit Score: 75.07  E-value: 1.09e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  649 RMD--PRAQTPVPGKGPFGRDELLRRKLIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLDKPS------ 720
Cdd:cd13391     1 RLDatALERASNPLAAEFKNLDLTTRRMIHEGPLTWRISKDKTLDLHVLLLEDLLVLLQKQDEKLVLKCHSKTAvgssds 80
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  721 ------VVSLQNLIVRDIANQAKGMFLISSGP--PEMYEVHAASRDDRTTWIRVIQQSVR 772
Cdd:cd13391    81 kqtfspVLKLNSVLIRSVATDKRALFIICTSKlgPQIYELVALTSSEKNTWMELLEEAVR 140
C1_p190RhoGEF cd20876
protein kinase C conserved region 1 (C1 domain) found in 190 kDa guanine nucleotide exchange ...
233-291 1.24e-15

protein kinase C conserved region 1 (C1 domain) found in 190 kDa guanine nucleotide exchange factor (p190RhoGEF) and similar proteins; p190RhoGEF, also called Rho guanine nucleotide exchange factor (RGNEF), Rho guanine nucleotide exchange factor 28 (ARHGEF28), or RIP2, is a brain-enriched, RhoA-specific guanine nucleotide exchange factor that regulates signaling pathways downstream of integrins and growth factor receptors. It is involved in axonal branching, synapse formation and dendritic morphogenesis, as well as in focal adhesion formation, cell motility and B-lymphocytes activation. In addition to the Dbl homology (DH)-PH domain, p190RhoGEF contains an N-terminal C1 (Protein kinase C conserved region 1) domain. This model corresponds to the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410426  Cd Length: 61  Bit Score: 72.08  E-value: 1.24e-15
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 755503406  233 DARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQ 291
Cdd:cd20876     1 KEKQSNGHQFVTGSFSGPTLCVVCDKPVTGKELLQCSNCTVNVHKGCKESAPPCTKKLQ 59
PH_LARG cd13390
Leukemia-associated Rho guanine nucleotide exchange factor Pleckstrin homology (PH) domain; ...
668-769 4.46e-14

Leukemia-associated Rho guanine nucleotide exchange factor Pleckstrin homology (PH) domain; LARG (also called RhoGEF12) belongs to regulator of G-protein signaling (RGS) domain-containing RhoGEFs that are RhoA-selective and directly activated by the Galpha12/13 family of heterotrimeric G proteins. RhoGEFs activate Rho GTPases regulating cytoskeletal structure, gene transcription, and cell migration. LARG contains a N-terminal extension, followed by Dbl homology (DH)-PH domains which bind and catalyze the exchange of GDP for GTP on RhoA in addition to a RGS domain. The active site of RhoA adopts two distinct GDP-excluding conformations among the four unique complexes in the asymmetric unit. The LARG PH domain also contains a potential protein-docking site. LARG forms a homotetramer via its DH domains. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275425  Cd Length: 138  Bit Score: 70.40  E-value: 4.46e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  668 ELLRRKLIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLDK------------PSVVSLQNLIVRDIANQ 735
Cdd:cd13390    20 DLTKRKMIHEGPLTWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKilastadskhtfSPVIKLNTVLVRQVATD 99
                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 755503406  736 AKGMFLI--SSGPPEMYEVHAASRDDRTTWIRVIQQ 769
Cdd:cd13390   100 NKAFFVIsmSENGAQIYELVAQTVSEKTVWQDLITR 135
PH smart00233
Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The ...
674-772 4.07e-10

Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The domain family possesses multiple functions including the abilities to bind inositol phosphates, and various proteins. PH domains have been found to possess inserted domains (such as in PLC gamma, syntrophins) and to be inserted within other domains. Mutations in Brutons tyrosine kinase (Btk) within its PH domain cause X-linked agammaglobulinaemia (XLA) in patients. Point mutations cluster into the positively charged end of the molecule around the predicted binding site for phosphatidylinositol lipids.


Pssm-ID: 214574 [Multi-domain]  Cd Length: 102  Bit Score: 57.94  E-value: 4.07e-10
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406    674 LIHEGCLLWKTATGR--FKDVLLLLMTDVLVFLQEKDQKYIFTsldKPSVVSLQNLIVRDIANQ----AKGMFLISSGPP 747
Cdd:smart00233    1 VIKEGWLYKKSGGGKksWKKRYFVLFNSTLLYYKSKKDKKSYK---PKGSIDLSGCTVREAPDPdsskKPHCFEIKTSDR 77
                            90       100
                    ....*....|....*....|....*
gi 755503406    748 EMYEVHAASRDDRTTWIRVIQQSVR 772
Cdd:smart00233   78 KTLLLQAESEEEREKWVEALRKAIA 102
PH_p115RhoGEF cd14679
Rho guanine nucleotide exchange factor Pleckstrin homology domain; p115RhoGEF (also called LSC, ...
664-771 1.47e-09

Rho guanine nucleotide exchange factor Pleckstrin homology domain; p115RhoGEF (also called LSC, GEF1 or LBCL2) belongs to regulator of G-protein signaling (RGS) domain-containing RhoGEFs that are RhoA-selective and directly activated by the Galpha12/13 family of heterotrimeric G proteins. In addition to the Dbl homology (DH)-PH domain, p115RhoGEF contains an N-terminal RGS (Regulator of G-protein signalling) domain. The DH-PH domains bind and catalyze the exchange of GDP for GTP on RhoA. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275429  Cd Length: 125  Bit Score: 57.16  E-value: 1.47e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  664 FGRDELLRRKLIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLDKPS------------VVSLQNLIVRD 731
Cdd:cd14679     1 FKNIDITKKKLVHEGPLTWRVTKDKAIEVHVLLLDDLLVLLQKQDERLVLKCHSRTTtptpdgkqmlspIIKLNSAMTRE 80
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 755503406  732 IANQAKGMFLISS--GPPEMYEVHAASRDDRTTWIRVIQQSV 771
Cdd:cd14679    81 VATDRKAFYVIFTweQGAQIYELVAQTVSERKNWCALISETA 122
C1 smart00109
Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains); Some bind phorbol ...
240-286 1.82e-08

Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains); Some bind phorbol esters and diacylglycerol. Some bind RasGTP. Zinc-binding domains.


Pssm-ID: 197519  Cd Length: 50  Bit Score: 51.31  E-value: 1.82e-08
                            10        20        30        40        50
                    ....*....|....*....|....*....|....*....|....*....|
gi 755503406    240 HLFTTISVSGMTMCYACNKSI--TAKEALICPTCNVTIHNRCKDTL-ANC 286
Cdd:smart00109    1 HKHVFRTFTKPTFCCVCRKSIwgSFKQGLRCSECKVKCHKKCADKVpKAC 50
PH pfam00169
PH domain; PH stands for pleckstrin homology.
674-772 1.87e-08

PH domain; PH stands for pleckstrin homology.


Pssm-ID: 459697 [Multi-domain]  Cd Length: 105  Bit Score: 53.34  E-value: 1.87e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406   674 LIHEGCLL--WKTATGRFKDVLLLLMTDVLVFLQEKDqkyIFTSLDKPSVVSLQNLIVRDIANQAKG----MFLI---SS 744
Cdd:pfam00169    1 VVKEGWLLkkGGGKKKSWKKRYFVLFDGSLLYYKDDK---SGKSKEPKGSISLSGCEVVEVVASDSPkrkfCFELrtgER 77
                           90       100
                   ....*....|....*....|....*...
gi 755503406   745 GPPEMYEVHAASRDDRTTWIRVIQQSVR 772
Cdd:pfam00169   78 TGKRTYLLQAESEEERKDWIKAIQSAIR 105
ROM1 COG5422
RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction ...
409-709 6.36e-08

RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction mechanisms];


Pssm-ID: 227709 [Multi-domain]  Cd Length: 1175  Bit Score: 57.21  E-value: 6.36e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  409 DEKDFEADSWSLAVDSSFLQQHKKEVMKKQDVIYELIQTELHHVRTLKImTRLFRTGMLEELQMEPE-----VVQGLFPC 483
Cdd:COG5422   457 DKFDEEKNLWTLSVPKEVWESLPKQEIKRQEAIYEVIYTERDFVKDLEY-LRDTWIKPLEESNIIPEnarrnFIKHVFAN 535
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  484 VDELSDIHTRFLNQLleRRRQALCPgstrnfVIHRLGDLLIsqfsgsnaeqmrktysEFCSRHTKALKLYKE-LYAR--- 559
Cdd:COG5422   536 INEIYAVNSKLLKAL--TNRQCLSP------IVNGIADIFL----------------DYVPKFEPFIKYGASqPYAKyef 591
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  560 ------DKRFQQFIRKMTRSAVLKRHGVQECILLVTQRITKYPVLINRILQNSHGVEEEYQDLASALGLVKELLSNVDQD 633
Cdd:COG5422   592 ereksvNPNFARFDHEVERLDESRKLELDGYLTKPTTRLARYPLLLEEVLKFTDPDNPDTEDIPKVIDMLREFLSRLNFE 671
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  634 VHELEKEARL----QEIYNRMDPRAQTPvpgkgpfgRDEllRRKLIHEGCL----LWKTATGRFKDVLLLLMTDVLVFLQ 705
Cdd:COG5422   672 SGKAENRGDLfhlnQQLLFKPEYVNLGL--------NDE--YRKIIFKGVLkrkaKSKTDGSLRGDIQFFLLDNMLLFCK 741

                  ....
gi 755503406  706 EKDQ 709
Cdd:COG5422   742 AKAV 745
C1_ARHGEF18-like cd20879
protein kinase C conserved region 1 (C1 domain) found in uncharacterized Rho guanine ...
237-287 7.92e-07

protein kinase C conserved region 1 (C1 domain) found in uncharacterized Rho guanine nucleotide exchange factor 18 (ARHGEF18)-like proteins; The family includes a group of uncharacterized proteins that show high sequence similarity to vertebrate ARHGEF18, which is also called 114 kDa Rho-specific guanine nucleotide exchange factor (p114-Rho-GEF), p114RhoGEF, or septin-associated RhoGEF (SA-RhoGEF). ARHGEF18 acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. ARHGEF18 also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Members of this family contain C1, RhoGEF or Dbl-homologous (DH), and Pleckstrin Homology (PH) domains, as well as a DUF5401 domain. This model corresponds to the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410429  Cd Length: 53  Bit Score: 47.12  E-value: 7.92e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 755503406  237 TNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCT 287
Cdd:cd20879     1 VNGHQLVPGTFSSCATCSLCSKPLQNRNGLQCLNCAVNVHKNCKTLLTECS 51
C1 cd00029
protein kinase C conserved region 1 (C1 domain) superfamily; The C1 domain is a cysteine-rich ...
240-286 2.17e-06

protein kinase C conserved region 1 (C1 domain) superfamily; The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains. It contains the motif HX12CX2CXnCX2CX4HX2CX7C, where C and H are cysteine and histidine, respectively; X represents other residues; and n is either 13 or 14. C1 has a globular fold with two separate Zn(2+)-binding sites. It was originally discovered as lipid-binding modules in protein kinase C (PKC) isoforms. C1 domains that bind and respond to phorbol esters (PE) and diacylglycerol (DAG) are referred to as typical, and those that do not respond to PE and DAG are deemed atypical. A C1 domain may also be referred to as PKC or non-PKC C1, based on the parent protein's activity. Most C1 domain-containing non-PKC proteins act as lipid kinases and scaffolds, except PKD which acts as a protein kinase. PKC C1 domains play roles in membrane translocation and activation of the enzyme.


Pssm-ID: 410341  Cd Length: 50  Bit Score: 45.59  E-value: 2.17e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 755503406  240 HLFTTISVSGMTMCYACNKSIT--AKEALICPTCNVTIHNRCKDTLA-NC 286
Cdd:cd00029     1 HRFVPTTFSSPTFCDVCGKLIWglFKQGLKCSDCGLVCHKKCLDKAPsPC 50
COG4913 COG4913
Uncharacterized conserved protein, contains a C-terminal ATPase domain [Function unknown];
941-1086 5.19e-06

Uncharacterized conserved protein, contains a C-terminal ATPase domain [Function unknown];


Pssm-ID: 443941 [Multi-domain]  Cd Length: 1089  Bit Score: 50.68  E-value: 5.19e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  941 LINLYGLLHGLQAVVVQQERLMEALFPEgPERWEKLsRANSRDGEAGRAAVASVTPEKQATELALLQRQHTLLQEELRRc 1020
Cdd:COG4913   230 LVEHFDDLERAHEALEDAREQIELLEPI-RELAERY-AAARERLAELEYLRAALRLWFAQRRLELLEAELEELRAELAR- 306
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 755503406 1021 qrlgeerateagsLEARLRESEQARALLEREAEEIRRQLAALGQN--EPLPAEAPWARRPLDPRRRSL 1086
Cdd:COG4913   307 -------------LEAELERLEARLDALREELDELEAQIRGNGGDrlEQLEREIERLERELEERERRR 361
C1_dGM13116p-like cd20831
protein kinase C conserved region 1 (C1 domain) found in Drosophila melanogaster GM13116p and ...
238-279 8.83e-06

protein kinase C conserved region 1 (C1 domain) found in Drosophila melanogaster GM13116p and similar proteins; This group contains uncharacterized proteins including Drosophila melanogaster GM13116p and Caenorhabditis elegans hypothetical protein R11G1.4, both of which contain C2 (a calcium-binding domain) and C1 domains. This model describes the C1 domain, a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410381  Cd Length: 58  Bit Score: 44.25  E-value: 8.83e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 755503406  238 NGHLFTTISVSGMTMCYACNKSIT---AKEALICPTCNVTIHNRC 279
Cdd:cd20831     4 NDHTFVATHFKGGPSCAVCNKLIPgrfGKQGYQCRDCGLICHKRC 48
C1_Myosin-IXb cd20884
protein kinase C conserved region 1 (C1 domain) found in unconventional myosin-IXb and similar ...
237-279 4.25e-05

protein kinase C conserved region 1 (C1 domain) found in unconventional myosin-IXb and similar proteins; Myosin-IXb, also called unconventional myosin-9b (Myo9b), is an actin-dependent motor protein of the unconventional myosin IX class. It is expressed abundantly in tissues of the immune system, like lymph nodes, thymus, and spleen, and in several immune cells including dendritic cells, macrophages and CD4+ T cells. Myosin-IXb contains a Ras-associating (RA) domain, a motor domain, a protein kinase C conserved region 1 (C1), and a Rho GTPase activating (RhoGAP) domain. Myosin-IXb acts as a motorized signaling molecule that links Rho signaling to the dynamic actin cytoskeleton. It regulates leukocyte migration by controlling RhoA signaling. Myosin-IXb is also involved in the development of autoimmune diseases, including rheumatoid arthritis, systemic lupus erythematosus, and type 1 diabetes. Moreover, Myosin-IXb is a ROBO-interacting protein that suppresses RhoA activity in lung cancer cells. This model corresponds to the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410434  Cd Length: 58  Bit Score: 42.16  E-value: 4.25e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 755503406  237 TNGHLFTTISVSGMTMCYACNKSITAKE-ALICPTCNVTIHNRC 279
Cdd:cd20884     3 YNGHVFTSYQVNIMQSCEQCSSYIWAMEkALLCSVCKMTCHKKC 46
COG4913 COG4913
Uncharacterized conserved protein, contains a C-terminal ATPase domain [Function unknown];
934-1077 6.82e-05

Uncharacterized conserved protein, contains a C-terminal ATPase domain [Function unknown];


Pssm-ID: 443941 [Multi-domain]  Cd Length: 1089  Bit Score: 47.22  E-value: 6.82e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  934 QEEVLQPLINLYGLLHGLQAVVVQQERLMEALFPEGPERWEKLSRANSRDGEAGRAavasvtpeKQATELALLQRQHTLL 1013
Cdd:COG4913   250 QIELLEPIRELAERYAAARERLAELEYLRAALRLWFAQRRLELLEAELEELRAELA--------RLEAELERLEARLDAL 321
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 755503406 1014 QEELRRCQRlgeERATEAG----SLEARLRESEQARALLEREAEEIRRQLAALGQNEPLPAEAPWARR 1077
Cdd:COG4913   322 REELDELEA---QIRGNGGdrleQLEREIERLERELEERERRRARLEALLAALGLPLPASAEEFAALR 386
C1_MgcRacGAP cd20821
protein kinase C conserved region 1 (C1 domain) found in male germ cell RacGap (MgcRacGAP) and ...
240-283 7.78e-05

protein kinase C conserved region 1 (C1 domain) found in male germ cell RacGap (MgcRacGAP) and similar proteins; MgcRacGAP, also called Rac GTPase-activating protein 1 (RACGAP1) or protein CYK4, plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin MKLP1, which is critical for the structure of the central spindle by promoting microtuble bundling; and ii) after phosphorylation by aurora B, MgcRacGAP becomes an effective regulator of RhoA and plays an important role in the assembly of the contractile ring and the initiation of cytokinesis. MgcRacGAP-like proteins contain an N-terminal C1 domain, and a C-terminal RhoGAP domain. This model corresponds to the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410371  Cd Length: 55  Bit Score: 41.24  E-value: 7.78e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 755503406  240 HLFTTISVSGMTMCYACNKSIT-AKEALICPTCNVTIHNRCKDTL 283
Cdd:cd20821     3 HRFVSKTVIKPETCVVCGKRIKfGKKALKCKDCRVVCHPDCKDKL 47
C1_Myosin-IX cd20818
protein kinase C conserved region 1 (C1 domain) found in the unconventional myosin-IX family; ...
238-279 1.42e-04

protein kinase C conserved region 1 (C1 domain) found in the unconventional myosin-IX family; Myosins IX (Myo9) is a class of unique motor proteins with a common structure of an N-terminal extension preceding a myosin head homologous to the Ras-association (RA) domain, a head (motor) domain, a neck with IQ motifs that bind light chains, and a C-terminal tail containing cysteine-rich zinc binding (C1) and Rho-GTPase activating protein (RhoGAP) domains. There are two genes for myosins IX in humans, IXa and IXb, that are different in their expression and localization. IXa is expressed abundantly in brain and testis, and IXb is expressed abundantly in tissues of the immune system. This model corresponds to the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410368  Cd Length: 56  Bit Score: 40.75  E-value: 1.42e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 755503406  238 NGHLFTTISVSGMTMCYACNKSITAKE-ALICPTCNVTIHNRC 279
Cdd:cd20818     2 NGHKFATVQFNIPTYCEVCNSFIWLMEkGLVCQVCKFTCHKKC 44
EnvC COG4942
Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, ...
957-1101 3.07e-04

Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 443969 [Multi-domain]  Cd Length: 377  Bit Score: 44.37  E-value: 3.07e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406  957 QQERLMEALFPEGPERWEK-------LSRANSRDGEAGRAAVASVTPEKQ--ATELALLQRQHTLLQEELRRCQRLGEER 1027
Cdd:COG4942   118 RQPPLALLLSPEDFLDAVRrlqylkyLAPARREQAEELRADLAELAALRAelEAERAELEALLAELEEERAALEALKAER 197
                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 755503406 1028 ATEAGSLEARLRESEQARALLEREAEEIRRQLAALGQNEPLPAEAPWARRPLDPRRR-SLPAGDALYLSFNPPQP 1101
Cdd:COG4942   198 QKLLARLEKELAELAAELAELQQEAEELEALIARLEAEAAAAAERTPAAGFAALKGKlPWPVSGRVVRRFGERDG 272
Smc COG1196
Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning]; ...
997-1062 1.15e-03

Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 440809 [Multi-domain]  Cd Length: 983  Bit Score: 43.00  E-value: 1.15e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 755503406  997 EKQATELALLQRQHTLLQEELRRCQRLGEERATEAGSLEARLRESEQARALLEREAEEIRRQLAAL 1062
Cdd:COG1196   319 EELEEELAELEEELEELEEELEELEEELEEAEEELEEAEAELAEAEEALLEAEAELAEAEEELEEL 384
TolC COG1538
Outer membrane protein TolC [Cell wall/membrane/envelope biogenesis];
1003-1098 4.59e-03

Outer membrane protein TolC [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441147 [Multi-domain]  Cd Length: 367  Bit Score: 40.79  E-value: 4.59e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755503406 1003 LALLQRQHTLLQEELRRCQRL---GEERATEAGSLEARLRESEQARALLEREAEEIRRQLAAL-GQNEPLPAEAPWARRP 1078
Cdd:COG1538    92 LALAEENLALAEELLELARARyeaGLASRLDVLQAEAQLAQARAQLAQAEAQLAQARNALALLlGLPPPAPLDLPDPLPP 171
                          90       100
                  ....*....|....*....|
gi 755503406 1079 LDPRRRSLPAGDALYLSFNP 1098
Cdd:COG1538   172 LPPLPPSLPGLPSEALERRP 191
C1_RASSF5 cd20886
protein kinase C conserved region 1 (C1 domain) found in Ras association domain-containing ...
239-281 5.05e-03

protein kinase C conserved region 1 (C1 domain) found in Ras association domain-containing protein 5 (RASSF5) and similar proteins; RASSF5, also called new ras effector 1 (NORE1), or regulator for cell adhesion and polarization enriched in lymphoid tissues (RAPL), is a member of a family of RAS effectors, of which there are currently 8 members (RASSF1-8), all containing a Ras-association (RA) domain of the Ral-GDS/AF6 type. It is expressed as three transcripts (A-C) via differential promoter usage and alternative splicing. RASSF5A is a pro-apoptotic Ras effector and functions as a Ras regulated tumor suppressor. RASSF5C is regulated by Ras related protein and modulates cellular adhesion. RASSF5 is a potential tumor suppressor that seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. It contains a C1 domain, which is descibed in this model. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410436  Cd Length: 50  Bit Score: 36.21  E-value: 5.05e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 755503406  239 GHLFTTISVSGmTMCYACNKSITAkEALICPTCNVTIHNRCKD 281
Cdd:cd20886     3 GHRFEPGALGP-GWCDLCGRYILS-QALRCTNCKYTCHSECRD 43
C1_Stac cd20817
protein kinase C conserved region 1 (C1 domain) found in the SH3 and cysteine-rich ...
251-288 6.36e-03

protein kinase C conserved region 1 (C1 domain) found in the SH3 and cysteine-rich domain-containing protein (Stac) family; Stac proteins are putative adaptor proteins that are important for neuronal function. There are three mammalian members (Stac1, Stac2 and Stac3) of this family. Stac1 and Stac3 contain two SH3 domains while Stac2 contains a single SH3 domain at the C-terminus. Stac1 and Stac2 have been found to be expressed differently in mature dorsal root ganglia (DRG) neurons. Stac1 is mainly expressed in peptidergic neurons while Stac2 is found in a subset of nonpeptidergic and all trkB+ neurons. Stac proteins contain a cysteine-rich C1 domain and one or two SH3 domains at the C-terminus. This model corresponds to the C1 domain. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410367  Cd Length: 51  Bit Score: 35.77  E-value: 6.36e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 755503406  251 TMCYACNKSI--TAKEALICPTCNVTIHNRCKDTLANCTK 288
Cdd:cd20817    12 TFCDVCKELLvgLSKQGLRCKNCKMNVHHKCQEGVPDCSG 51
C1_Munc13 cd20807
protein kinase C conserved region 1 (C1 domain) found in the Munc13 family; The Munc13 gene ...
240-286 6.39e-03

protein kinase C conserved region 1 (C1 domain) found in the Munc13 family; The Munc13 gene family encodes a family of neuron-specific, synaptic molecules that bind to syntaxin, an essential mediator of neurotransmitter release. Munc13-1 is a component of presynaptic active zones in which it acts as an essential synaptic vesicle priming protein. Munc13-2 is essential for normal release probability at hippocampal mossy fiber synapses. Munc13-3 is almost exclusively expressed in the cerebellum. It acts as a tumor suppressor and plays a critical role in the formation of release sites with calcium channel nanodomains. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.


Pssm-ID: 410357  Cd Length: 53  Bit Score: 35.92  E-value: 6.39e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 755503406  240 HLFTTISVSGMTMCYACNKSI--TAKEALICPTCNVTIHNRCKDTL-ANC 286
Cdd:cd20807     1 HNFEVWTATTPTYCYECEGLLwgIARQGVRCTECGVKCHEKCKDLLnADC 50
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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