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Conserved domains on  [gi|704272444|ref|XP_010154526|]
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PREDICTED: cholesterol 25-hydroxylase [Eurypyga helias]

Protein Classification

sterol desaturase family protein( domain architecture ID 10513792)

sterol desaturase family protein is an oxidoreductase, similar to C-5 sterol desaturase, which catalyzes a dehydrogenation reaction to introduce a C5-6 double bond into substrates such as lathosterol and the B ring of ergosterol

EC:  1.-.-.-
Gene Ontology:  GO:0006629|GO:0016491

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 133-268 9.40e-27

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


:

Pssm-ID: 397991  Cd Length: 134  Bit Score: 101.35  E-value: 9.40e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 704272444  133 VGVCLLLFDFEYFLWHLLHHKVPWLYKTFHKVHHKHVSTFALTTQYSSVWELLSLGFFAAINPVLLGCHPLTEMVFFLVN 212
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHRLLHRLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPLLAFLLGLLLG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 704272444  213 IWLSVEDHSGYDLPwstHRLVPFGLYGGAPHHDLHHLKF-KSNYAPYFTHWDKLFGT 268
Cdd:pfam04116  81 TLWYLFIHSGLLFP---LPWLLKRLLGTPRFHRLHHSKNeEYNFGVTFPLWDRLFGT 134
 
Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 133-268 9.40e-27

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 101.35  E-value: 9.40e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 704272444  133 VGVCLLLFDFEYFLWHLLHHKVPWLYKTFHKVHHKHVSTFALTTQYSSVWELLSLGFFAAINPVLLGCHPLTEMVFFLVN 212
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHRLLHRLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPLLAFLLGLLLG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 704272444  213 IWLSVEDHSGYDLPwstHRLVPFGLYGGAPHHDLHHLKF-KSNYAPYFTHWDKLFGT 268
Cdd:pfam04116  81 TLWYLFIHSGLLFP---LPWLLKRLLGTPRFHRLHHSKNeEYNFGVTFPLWDRLFGT 134
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 135-269 1.55e-17

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 77.67  E-value: 1.55e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 704272444 135 VCLLLFDFEYFLWHLLHHKVPWLYKtFHKVHHKHVSTFALTTQYSSVWELLSLGFFAAINPVLLGCHPLTEMVFFLVNIW 214
Cdd:COG3000    4 LAFLLLDFVYYWLHRLSHRVPLLWR-FHAVHHSSERMNLLTALRFHPLEILLSALLFLLPLALLGFPPEAVLLYLALNLL 82

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 704272444 215 LSVEDHSGYDLPWstHRLVPFgLYGGAPHHDLHHLK----FKSNYAPYFTHWDKLFGTF 269
Cdd:COG3000   83 YQFFNHSNIRLPL--DGPLEY-VFVTPSHHRVHHSRnpeyLDKNYGGILSIWDRLFGTY 138
PLN02869 PLN02869
fatty aldehyde decarbonylase
 129-272 3.18e-03

fatty aldehyde decarbonylase


Pssm-ID: 166510 [Multi-domain]  Cd Length: 620  Bit Score: 38.67  E-value: 3.18e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 704272444 129 VLLEVGVCLLLFdfeYFLWHLLHHKvpWLYKTFHKVHHKHVSTFALTTQYSSVWELLSLGFFAAInPVLLGCHPLTEMV- 207
Cdd:PLN02869 131 ILLHMGPVEFLY---YWLHRALHHH--YLYSRYHSHHHSSIVTEPITSVIHPFAEHIAYFLLFAI-PLLTTIFTGTASIa 204

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 704272444 208 -FFLVNIWLSVEDHSGYdlpwSTHRLVPFGLYGGAP----------HHDLHHLKFKSNYAPYFTHWDKLFGTFTES 272
Cdd:PLN02869 205 aFFGYISYIDFMNNMGH----CNFELIPKWLFSIFPplkylmytpsYHSLHHTQFRTNYSLFMPIYDYIYGTMDKS 276
 
Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 133-268 9.40e-27

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 101.35  E-value: 9.40e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 704272444  133 VGVCLLLFDFEYFLWHLLHHKVPWLYKTFHKVHHKHVSTFALTTQYSSVWELLSLGFFAAINPVLLGCHPLTEMVFFLVN 212
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHRLLHRLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPLLAFLLGLLLG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 704272444  213 IWLSVEDHSGYDLPwstHRLVPFGLYGGAPHHDLHHLKF-KSNYAPYFTHWDKLFGT 268
Cdd:pfam04116  81 TLWYLFIHSGLLFP---LPWLLKRLLGTPRFHRLHHSKNeEYNFGVTFPLWDRLFGT 134
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 135-269 1.55e-17

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 77.67  E-value: 1.55e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 704272444 135 VCLLLFDFEYFLWHLLHHKVPWLYKtFHKVHHKHVSTFALTTQYSSVWELLSLGFFAAINPVLLGCHPLTEMVFFLVNIW 214
Cdd:COG3000    4 LAFLLLDFVYYWLHRLSHRVPLLWR-FHAVHHSSERMNLLTALRFHPLEILLSALLFLLPLALLGFPPEAVLLYLALNLL 82

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 704272444 215 LSVEDHSGYDLPWstHRLVPFgLYGGAPHHDLHHLK----FKSNYAPYFTHWDKLFGTF 269
Cdd:COG3000   83 YQFFNHSNIRLPL--DGPLEY-VFVTPSHHRVHHSRnpeyLDKNYGGILSIWDRLFGTY 138
PLN02869 PLN02869
fatty aldehyde decarbonylase
 129-272 3.18e-03

fatty aldehyde decarbonylase


Pssm-ID: 166510 [Multi-domain]  Cd Length: 620  Bit Score: 38.67  E-value: 3.18e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 704272444 129 VLLEVGVCLLLFdfeYFLWHLLHHKvpWLYKTFHKVHHKHVSTFALTTQYSSVWELLSLGFFAAInPVLLGCHPLTEMV- 207
Cdd:PLN02869 131 ILLHMGPVEFLY---YWLHRALHHH--YLYSRYHSHHHSSIVTEPITSVIHPFAEHIAYFLLFAI-PLLTTIFTGTASIa 204

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 704272444 208 -FFLVNIWLSVEDHSGYdlpwSTHRLVPFGLYGGAP----------HHDLHHLKFKSNYAPYFTHWDKLFGTFTES 272
Cdd:PLN02869 205 aFFGYISYIDFMNNMGH----CNFELIPKWLFSIFPplkylmytpsYHSLHHTQFRTNYSLFMPIYDYIYGTMDKS 276
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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