|
Name |
Accession |
Description |
Interval |
E-value |
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
77-626 |
1.43e-177 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 511.06 E-value: 1.43e-177
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 77 STAVVHalSGRRFTYGELLGDVARARERLREavDGKPLTGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLS 156
Cdd:cd05941 2 RIAIVD--DGDSITYADLVARAARLANRLLA--LGKDLRGDRVAFLAPPSAEYVV---------AQLAIWRAGGVAVPLN 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 157 PPFPAPELQYILDQSAASVLVAspkfaakarevlatdlqqkpvyfelpkhtgsshgakeavsladedfsgAGLMLYTSGT 236
Cdd:cd05941 69 PSYPLAELEYVITDSEPSLVLD------------------------------------------------PALILYTSGT 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 237 TNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAVWKRfaapflp 316
Cdd:cd05941 101 TGRPKGVVLTHANLAANVRALVDAWRWTEDDVLLHVLPLHHVHGLVNALLCPLFAGASVEFLPKFDPKEVAIS------- 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 317 tngtaTTAPPISFFTVVPTVYSRLLASHKTLSPQMQAATSEAtsPANLRVNISGSAALPTPVKAAWAALSgGNVLLERYG 396
Cdd:cd05941 174 -----RLMPSITVFMGVPTIYTRLLQYYEAHFTDPQFARAAA--AERLRLMVSGSAALPVPTLEEWEAIT-GHTLLERYG 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 397 MTEVGMALSCGLAfADRVDASVGWPLPSVEARLVDMDTGDViepghelddHGRERVGEIQLRGPTVFREYWRNPDATAKE 476
Cdd:cd05941 246 MTEIGMALSNPLD-GERRPGTVGMPLPGVQARIVDEETGEP---------LPRGEVGEIQVRGPSVFKEYWNKPEATKEE 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 477 FVDDGgdgrgrWFKTGDVAIRREpvgaagrnshrqpwaqGPMYFIQGRKTADIIKTGGEKVSALEVERELLSLPQVAEAA 556
Cdd:cd05941 316 FTDDG------WFKTGDLGVVDE----------------DGYYWILGRSSVDIIKSGGYKVSALEIERVLLAHPGVSECA 373
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 557 VVAVPSGNWGQKVGAVIILDPDHAVkWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLVK 626
Cdd:cd05941 374 VIGVPDPDWGERVVAVVVLRAGAAA-LSLEELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
66-627 |
1.04e-108 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 335.24 E-value: 1.04e-108
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAV 145
Cdd:COG0318 4 LLRRAAARHPDRPALVFG--GRRLTYAELDARARRLAAALRALGVGP---GDRVALLLPNSPEFVV---------AFLAA 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASpkfaakarevlatdlqqkpvyfelpkhtgsshgakeavsladedfs 225
Cdd:COG0318 70 LRAGAVVVPLNPRLTAEELAYILEDSGARALVTA---------------------------------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 226 gagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADA 305
Cdd:COG0318 104 ---LILYTSGTTGRPKGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPLLAGATLVLLPRFDPER 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 306 VWKRFAapflptngtattAPPISFFTVVPTVYSRLLashktlspqmQAATSEATSPANLRVNISGSAALPTPVKAAWAAL 385
Cdd:COG0318 181 VLELIE------------RERVTVLFGVPTMLARLL----------RHPEFARYDLSSLRLVVSGGAPLPPELLERFEER 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 386 sGGNVLLERYGMTEVGMALSCGLA-FADRVDASVGWPLPSVEARLVDMDTGDViEPGHelddhgrerVGEIQLRGPTVFR 464
Cdd:COG0318 239 -FGVRIVEGYGLTETSPVVTVNPEdPGERRPGSVGRPLPGVEVRIVDEDGREL-PPGE---------VGEIVVRGPNVMK 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 465 EYWRNPDATAKEFVDdggdgrgRWFKTGDVAIRREpvgaagrnshrqpwaQGpMYFIQGRKtADIIKTGGEKVSALEVER 544
Cdd:COG0318 308 GYWNDPEATAEAFRD-------GWLRTGDLGRLDE---------------DG-YLYIVGRK-KDMIISGGENVYPAEVEE 363
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 545 ELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:COG0318 364 VLAAHPGVAEAAVVGVPDEKWGERVVAFVVLRPGAEL--DAEELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRAL 441
|
...
gi 573989775 625 VKA 627
Cdd:COG0318 442 RER 444
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
65-624 |
2.11e-86 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 278.68 E-value: 2.11e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 65 PIFEAVAAH--DPTSTAVvHALSGRRFTYGELLGDVARARERLReAVDGKPltGERVAFLIENSydyvvPEAhsrdVVMF 142
Cdd:PRK07514 4 NLFDALRAAfaDRDAPFI-ETPDGLRYTYGDLDAASARLANLLV-ALGVKP--GDRVAVQVEKS-----PEA----LALY 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 143 LAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPK---------FAAKAREVLATDLQQKPVYFELPKHTGSSHga 213
Cdd:PRK07514 71 LATLRAGAVFLPLNTAYTLAELDYFIGDAEPALVVCDPAnfawlskiaAAAGAPHVETLDADGTGSLLEAAAAAPDDF-- 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 214 kEAVSLADEDFSGaglMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGS 293
Cdd:PRK07514 149 -ETVPRGADDLAA---ILYTSGTTGRSKGAMLSHGNLLSNALTLVDYWRFTPDDVLIHALPIFHTHGLFVATNVALLAGA 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 294 SVEFMFPFNADAVWKRfaapfLPtngTATTappisfFTVVPTVYSRLLAShktlspqmQAATSEATspANLRVNISGSAA 373
Cdd:PRK07514 225 SMIFLPKFDPDAVLAL-----MP---RATV------MMGVPTFYTRLLQE--------PRLTREAA--AHMRLFISGSAP 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 374 LPTPVKAAWAALSgGNVLLERYGMTEVGMALSCGLAfADRVDASVGWPLPSVEARLVDMDTGDVIEPGhelddhgreRVG 453
Cdd:PRK07514 281 LLAETHREFQERT-GHAILERYGMTETNMNTSNPYD-GERRAGTVGFPLPGVSLRVTDPETGAELPPG---------EIG 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 454 EIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIrrepVGAAGRNShrqpwaqgpmyfIQGRKTaDIIKTG 533
Cdd:PRK07514 350 MIEVKGPNVFKGYWRMPEKTAEEFRADG------FFITGDLGK----IDERGYVH------------IVGRGK-DLIISG 406
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 534 GEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPR 613
Cdd:PRK07514 407 GYNVYPKEVEGEIDELPGVVESAVIGVPHPDFGEGVTAVVVPKPGAAL--DEAAILAALKGRLARFKQPKRVFFVDELPR 484
|
570
....*....|.
gi 573989775 614 NAMGKINKKQL 624
Cdd:PRK07514 485 NTMGKVQKNLL 495
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
69-624 |
1.13e-83 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 270.71 E-value: 1.13e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 69 AVAAHDPTSTAVVhaLSGRRFTYGELLGDVARARERLREAvdgkpltgERVAFLIEnsydyvvPEAHSrdVVMFLAVMAA 148
Cdd:PRK07787 8 AVAAAADIADAVR--IGGRVLSRSDLAGAATAVAERVAGA--------RRVAVLAT-------PTLAT--VLAVVGALIA 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 149 RAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLqqkpvyfelpkHTGSSHgakeavSLADEDFSGAG 228
Cdd:PRK07787 69 GVPVVPVPPDSGVAERRHILADSGAQAWLGPAPDDPAGLPHVPVRL-----------HARSWH------RYPEPDPDAPA 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 LMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAVwk 308
Cdd:PRK07787 132 LIVYTSGTTGPPKGVVLSRRAIAADLDALAEAWQWTADDVLVHGLPLFHVHGLVLGVLGPLRIGNRFVHTGRPTPEAY-- 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 309 rfaAPFLPTNGTAttappisFFTvVPTVYSRLLAShktlspqmqAATSEATSPANLRVniSGSAALPTPVKAAWAALSGG 388
Cdd:PRK07787 210 ---AQALSEGGTL-------YFG-VPTVWSRIAAD---------PEAARALRGARLLV--SGSAALPVPVFDRLAALTGH 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 389 NVlLERYGMTEVGMALSCgLAFADRVDASVGWPLPSVEARLVDmDTGDVIEpghelddHGRERVGEIQLRGPTVFREYWR 468
Cdd:PRK07787 268 RP-VERYGMTETLITLST-RADGERRPGWVGLPLAGVETRLVD-EDGGPVP-------HDGETVGELQVRGPTLFDGYLN 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 469 NPDATAKEFVDDGgdgrgrWFKTGDVAIrREPVGaagrnSHRqpwaqgpmyfIQGRKTADIIKTGGEKVSALEVERELLS 548
Cdd:PRK07787 338 RPDATAAAFTADG------WFRTGDVAV-VDPDG-----MHR----------IVGRESTDLIKSGGYRIGAGEIETALLG 395
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 573989775 549 LPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAvkwtPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK07787 396 HPGVREAAVVGVPDDDLGQRIVAYVVGADDVA----ADELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQL 467
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
71-621 |
6.91e-80 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 259.46 E-value: 6.91e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARA 150
Cdd:cd17631 5 ARRHPDRTALVFG--GRSLTYAELDERVNRLAHALRALGVAK---GDRVAVLSKNSPEFLE---------LLFAAARLGA 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 151 IAVPLSPPFPAPELQYILDQSAASVLVASPkfaakarevlatdlqqkpvyfelpkhtgsshgakeavsladedfsgAGLM 230
Cdd:cd17631 71 VFVPLNFRLTPPEVAYILADSGAKVLFDDL----------------------------------------------ALLM 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 231 lYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAVWKRF 310
Cdd:cd17631 105 -YTSGTTGRPKGAMLTHRNLLWNAVNALAALDLGPDDVLLVVAPLFHIGGLGVFTLPTLLRGGTVVILRKFDPETVLDLI 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 311 AapflptngtattAPPISFFTVVPTVYSRLLashktlspqmQAATSEATSPANLRVNISGSAALPTPVKAAWAALsgGNV 390
Cdd:cd17631 184 E------------RHRVTSFFLVPTMIQALL----------QHPRFATTDLSSLRAVIYGGAPMPERLLRALQAR--GVK 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 391 LLERYGMTEVGMaLSCGL--AFADRVDASVGWPLPSVEARLVDMDTGDViePGHElddhgrerVGEIQLRGPTVFREYWR 468
Cdd:cd17631 240 FVQGYGMTETSP-GVTFLspEDHRRKLGSAGRPVFFVEVRIVDPDGREV--PPGE--------VGEIVVRGPHVMAGYWN 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 469 NPDATAKEFVDDggdgrgrWFKTGDVAIRREpvgaAGRnshrqpwaqgpmYFIQGRKTaDIIKTGGEKVSALEVERELLS 548
Cdd:cd17631 309 RPEATAAAFRDG-------WFHTGDLGRLDE----DGY------------LYIVDRKK-DMIISGGENVYPAEVEDVLYE 364
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 573989775 549 LPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINK 621
Cdd:cd17631 365 HPAVAEVAVIGVPDEKWGEAVVAVVVPRPGAEL--DEDELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
66-625 |
7.62e-77 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 252.48 E-value: 7.62e-77
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVhaLSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAV 145
Cdd:cd05936 4 LLEEAARRFPDKTALI--FMGRKLTYRELDALAEAFAAGLQNLGVQP---GDRVALMLPNCPQFPI---------AYFGA 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAakarEVLATDlqqkpvyfelpkhtgssHGAKEAVSLADEDFS 225
Cdd:cd05936 70 LKAGAVVVPLNPLYTPRELEHILNDSGAKALIVAVSFT----DLLAAG-----------------APLGERVALTPEDVA 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 226 gagLMLYTSGTTNRPKGVLLPEPVITA---QCRSLLQAWEySPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFN 302
Cdd:cd05936 129 ---VLQYTSGTTGVPKGAMLTHRNLVAnalQIKAWLEDLL-EGDDVVLAALPLFHVFGLTVALLLPLALGATIVLIPRFR 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 303 ADAVWKrfaapflptngtATTAPPISFFTVVPTVYSRLLashktlspqmQAATSEATSPANLRVNISGSAALPTPVKAAW 382
Cdd:cd05936 205 PIGVLK------------EIRKHRVTIFPGVPTMYIALL----------NAPEFKKRDFSSLRLCISGGAPLPVEVAERF 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 383 AALSGGNvLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDMDTGDViEPGhelddhgreRVGEIQLRGPTV 462
Cdd:cd05936 263 EELTGVP-IVEGYGLTETSPVVAVNPLDGPRKPGSIGIPLPGTEVKIVDDDGEEL-PPG---------EVGELWVRGPQV 331
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 463 FREYWRNPDATAKEFVDDggdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGpMYFIQGRKTaDIIKTGGEKVSALEV 542
Cdd:cd05936 332 MKGYWNRPEETAEAFVDG-------WLRTGDIGYMDE---------------DG-YFFIVDRKK-DMIIVGGFNVYPREV 387
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 543 ERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKK 622
Cdd:cd05936 388 EEVLYEHPAVAEAAVVGVPDPYSGEAVKAFVVLKEGASL--TEEEIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRR 465
|
...
gi 573989775 623 QLV 625
Cdd:cd05936 466 ELR 468
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
229-619 |
2.62e-73 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 239.11 E-value: 2.62e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 LMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGtINAVLTPLFAGSSVEFMFPFNADAVWK 308
Cdd:cd04433 4 LILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGG-LFGLLGALLAGGTVVLLPKFDPEAALE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 309 RFAAPflptngtattapPISFFTVVPTVYSRLLashktlspqmQAATSEATSPANLRVNISGSAALPTPVKAAWAALsGG 388
Cdd:cd04433 83 LIERE------------KVTILLGVPTLLARLL----------KAPESAGYDLSSLRALVSGGAPLPPELLERFEEA-PG 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 389 NVLLERYGMTEVGMALSCGLAFAD-RVDASVGWPLPSVEARLVDMDTGDViEPGHelddhgrerVGEIQLRGPTVFREYW 467
Cdd:cd04433 140 IKLVNGYGLTETGGTVATGPPDDDaRKPGSVGRPVPGVEVRIVDPDGGEL-PPGE---------IGELVVRGPSVMKGYW 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 468 RNPDATAKEFvddgGDGrgrWFKTGDVAIRREpvgaAGRnshrqpwaqgpmYFIQGRKtADIIKTGGEKVSALEVERELL 547
Cdd:cd04433 210 NNPEATAAVD----EDG---WYRTGDLGRLDE----DGY------------LYIVGRL-KDMIKSGGENVYPAEVEAVLL 265
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 573989775 548 SLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKI 619
Cdd:cd04433 266 GHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADL--DAEELRAHVRERLAPYKVPRRVVFVDALPRTASGKI 335
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
75-626 |
1.54e-72 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 241.83 E-value: 1.54e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHALSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVP 154
Cdd:cd05926 1 PDAPALVVPGSTPALTYADLAELVDDLARQLAALGIKK---GDRVAIALPNGLEFVV---------AFLAAARAGAVVAP 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 155 LSPPFPAPELQYILDQSAASVLV-----------ASPKFAAKAREV--LATDLQQKPVYFELPKHTGSSHGAK-EAVSLA 220
Cdd:cd05926 69 LNPAYKKAEFEFYLADLGSKLVLtpkgelgpasrAASKLGLAILELalDVGVLIRAPSAESLSNLLADKKNAKsEGVPLP 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 221 DEDfsgaGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFP 300
Cdd:cd05926 149 DDL----ALILHTSGTTGRPKGVPLTHRNLAASATNITNTYKLTPDDRTLVVMPLFHVHGLVASLLSTLAAGGSVVLPPR 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 301 FNADAVWKRFAApFLPTngtattappisFFTVVPTVYSRLLASHKTLSPQmqaatseatSPANLRVNISGSAALPTPVKA 380
Cdd:cd05926 225 FSASTFWPDVRD-YNAT-----------WYTAVPTIHQILLNRPEPNPES---------PPPKLRFIRSCSASLPPAVLE 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 381 AWAALSGGNVlLERYGMTEVGMALSCG-LAFADRVDASVGWPLpSVEARLVDmDTGDVIEPGhelddhgreRVGEIQLRG 459
Cdd:cd05926 284 ALEATFGAPV-LEAYGMTEAAHQMTSNpLPPGPRKPGSVGKPV-GVEVRILD-EDGEILPPG---------VVGEICLRG 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 460 PTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAiRREPVGaagrnshrqpwaqgpmY-FIQGRKTaDIIKTGGEKVS 538
Cdd:cd05926 352 PNVTRGYLNNPEANAEAAFKDG------WFRTGDLG-YLDADG----------------YlFLTGRIK-ELINRGGEKIS 407
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 539 ALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGK 618
Cdd:cd05926 408 PLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLREGASV--TEEELRAFCRKHLAAFKVPKKVYFVDELPKTATGK 485
|
....*...
gi 573989775 619 INKKQLVK 626
Cdd:cd05926 486 IQRRKVAE 493
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
67-533 |
2.89e-71 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 236.44 E-value: 2.89e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 67 FEAVAAHDPTSTAVVHAlSGRRFTYGELLGDVARARERLREAvdG-KPltGERVAFLIENSYDYVVpeahsrdvvMFLAV 145
Cdd:pfam00501 1 LERQAARTPDKTALEVG-EGRRLTYRELDERANRLAAGLRAL--GvGK--GDRVAILLPNSPEWVV---------AFLAC 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKA-----------REVLATDLQQKPVYFELPKHTGSSHGAK 214
Cdd:pfam00501 67 LKAGAVYVPLNPRLPAEELAYILEDSGAKVLITDDALKLEEllealgklevvKLVLVLDRDPVLKEEPLPEEAKPADVPP 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 215 EAVSLADEDfSGAGLMlYTSGTTNRPKGVLLPEPVITAQCRSLLQ----AWEYSPADRLLHVLPLHHIHGTINAVLTPLF 290
Cdd:pfam00501 147 PPPPPPDPD-DLAYII-YTSGTTGKPKGVMLTHRNLVANVLSIKRvrprGFGLGPDDRVLSTLPLFHDFGLSLGLLGPLL 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 291 AGSSVefmfpfnadAVWKRFAAPFLPTNGTATTAPPISFFTVVPTVYSRLLAShktLSPQMQAATSeatspanLRVNISG 370
Cdd:pfam00501 225 AGATV---------VLPPGFPALDPAALLELIERYKVTVLYGVPTLLNMLLEA---GAPKRALLSS-------LRLVLSG 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 371 SAALPTPVKAAWAALsGGNVLLERYGMTEVGMALSCGLAFADR--VDASVGWPLPSVEARLVDMDTGDVIEPGHelddhg 448
Cdd:pfam00501 286 GAPLPPELARRFREL-FGGALVNGYGLTETTGVVTTPLPLDEDlrSLGSVGRPLPGTEVKIVDDETGEPVPPGE------ 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 449 rerVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaAGRnshrqpwaqgpmYFIQGRKtAD 528
Cdd:pfam00501 359 ---PGELCVRGPGVMKGYLNDPELTAEAFDEDG------WYRTGDLGRRDE----DGY------------LEIVGRK-KD 412
|
....*
gi 573989775 529 IIKTG 533
Cdd:pfam00501 413 QIKLG 417
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
66-624 |
5.01e-70 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 236.24 E-value: 5.01e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVhaLSGRRFTYGELLGDVARARERLREA-VDGkpltGERVAFLIENSYDYVVpeahsrdvvMFLA 144
Cdd:PRK06187 11 ILRHGARKHPDKEAVY--FDGRRTTYAELDERVNRLANALRALgVKK----GDRVAVFDWNSHEYLE---------AYFA 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 145 VMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLA-TDLQQKPVYFE---LPKHTGSSHGAKEAVSLA 220
Cdd:PRK06187 76 VPKIGAVLHPINIRLKPEEIAYILNDAEDRVVLVDSEFVPLLAAILPqLPTVRTVIVEGdgpAAPLAPEVGEYEELLAAA 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 221 DEDF-------SGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTiNAVLTPLFAGS 293
Cdd:PRK06187 156 SDTFdfpdideNDAAAMLYTSGTTGHPKGVVLSHRNLFLHSLAVCAWLKLSRDDVYLVIVPMFHVHAW-GLPYLALMAGA 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 294 SVEFMFPFNADAVWKRFAAPflptngtattapPISFFTVVPTVYSRLLashKTLSPQMQAATSeatspanLRVNISGSAA 373
Cdd:PRK06187 235 KQVIPRRFDPENLLDLIETE------------RVTFFFAVPTIWQMLL---KAPRAYFVDFSS-------LRLVIYGGAA 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 374 LPTPVKAAWAALSGGNvLLERYGMTEVGMALSCgLAFADRVDA------SVGWPLPSVEARLVDmDTGDVIEPGhelddh 447
Cdd:PRK06187 293 LPPALLREFKEKFGID-LVQGYGMTETSPVVSV-LPPEDQLPGqwtkrrSAGRPLPGVEARIVD-DDGDELPPD------ 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 448 GRErVGEIQLRGPTVFREYWRNPDATAKEFVDDggdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGpmY-FIQGRKT 526
Cdd:PRK06187 364 GGE-VGEIIVRGPWLMQGYWNRPEATAETIDGG-------WLHTGDVGYIDE---------------DG--YlYITDRIK 418
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 527 aDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMK 606
Cdd:PRK06187 419 -DVIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATL--DAKELRAFLRGRLAKFKLPKRIA 495
|
570
....*....|....*...
gi 573989775 607 IVNHIPRNAMGKINKKQL 624
Cdd:PRK06187 496 FVDELPRTSVGKILKRVL 513
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
79-620 |
5.74e-60 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 208.22 E-value: 5.74e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 79 AVVHALSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVPeahsrdvvmFLAVMAARAIAVPLSPP 158
Cdd:cd05911 1 AQIDADTGKELTYAQLRTLSRRLAAGLRKLGLKK---GDVVGIISPNSTYYPPV---------FLGCLFAGGIFSAANPI 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 159 FPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYfeLPKHTGSSHGAKEAVslaDEDFSGAG---------- 228
Cdd:cd05911 69 YTADELAHQLKISKPKVIFTDPDGLEKVKEAAKELGPKDKII--VLDDKPDGVLSIEDL---LSPTLGEEdedlppplkd 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 ------LMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAW--EYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVefMFP 300
Cdd:cd05911 144 gkddtaAILYSSGTTGLPKGVCLSHRNLIANLSQVQTFLygNDGSNDVILGFLPLYHIYGLFTTLASLLNGATVI--IMP 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 301 fnadavwKRFAAPFLptngTATTAPPISFFTVVPTVYSRLLASHKTLSPQMQaatseatspaNLRVNISGSAALPTPVKA 380
Cdd:cd05911 222 -------KFDSELFL----DLIEKYKITFLYLVPPIAAALAKSPLLDKYDLS----------SLRVILSGGAPLSKELQE 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 381 AWAALSGGNVLLERYGMTEVGMALSCgLAFADRVDASVGWPLPSVEARLVDMDTGDVIEPGhelddhgreRVGEIQLRGP 460
Cdd:cd05911 281 LLAKRFPNATIKQGYGMTETGGILTV-NPDGDDKPGSVGRLLPNVEAKIVDDDGKDSLGPN---------EPGEICVRGP 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 461 TVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaagrNSHrqpwaqgpmYFIQGRKTaDIIKTGGEKVSAL 540
Cdd:cd05911 351 QVMKGYYNNPEATKETFDEDG------WLHTGDIGYFDE-------DGY---------LYIVDRKK-ELIKYKGFQVAPA 407
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 541 EVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHavKWTPLEMRRALKSRLAGYKipQVMK---IVNHIPRNAMG 617
Cdd:cd05911 408 ELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPGE--KLTEKEVKDYVAKKVASYK--QLRGgvvFVDEIPKSASG 483
|
...
gi 573989775 618 KIN 620
Cdd:cd05911 484 KIL 486
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
86-631 |
1.22e-54 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 195.60 E-value: 1.22e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 86 GRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENsydyvVPEAhsrdVVMFLAVMAARAIAVPLSPPFPAPELQ 165
Cdd:PRK05605 55 GATTTYAELGKQVRRAAAGLRALGVRP---GDRVAIVLPN-----CPQH----IVAFYAVLRLGAVVVEHNPLYTAHELE 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 166 YILDQSAASVLVASPKFAAKARE---------VLATDL-------QQKPVYFELPKH-------TGSSHGAKEAVSLADE 222
Cdd:PRK05605 123 HPFEDHGARVAIVWDKVAPTVERlrrttpletIVSVNMiaampllQRLALRLPIPALrkaraalTGPAPGTVPWETLVDA 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 223 DFSGAG--------------LMLYTSGTTNRPKGVLLPEPVITAQCRSLlQAWEYSPAD---RLLHVLPLHHIHG-TINA 284
Cdd:PRK05605 203 AIGGDGsdvshprptpddvaLILYTSGTTGKPKGAQLTHRNLFANAAQG-KAWVPGLGDgpeRVLAALPMFHAYGlTLCL 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 285 VLTPLFAGSSVefMFP-FNAD---AVWKRFAAPFLPTngtattappisfftvVPTVYSRLlashktlspqMQAATSEATS 360
Cdd:PRK05605 282 TLAVSIGGELV--LLPaPDIDlilDAMKKHPPTWLPG---------------VPPLYEKI----------AEAAEERGVD 334
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 361 PANLRVNISGSAALPTPVKAAWAALSGGNvLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDMDTgdvieP 440
Cdd:PRK05605 335 LSGVRNAFSGAMALPVSTVELWEKLTGGL-LVEGYGLTETSPIIVGNPMSDDRRPGYVGVPFPDTEVRIVDPED-----P 408
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 441 GHELDDhGRErvGEIQLRGPTVFREYWRNPDATAKEFVDDggdgrgrWFKTGDVAIRREPvgaagrnshrqpwaqgpmYF 520
Cdd:PRK05605 409 DETMPD-GEE--GELLVRGPQVFKGYWNRPEETAKSFLDG-------WFRTGDVVVMEED------------------GF 460
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 521 IQGR-KTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVKwtPLEMRRALKSRLAGY 599
Cdd:PRK05605 461 IRIVdRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAALD--PEGLRAYCREHLTRY 538
|
570 580 590
....*....|....*....|....*....|..
gi 573989775 600 KIPQVMKIVNHIPRNAMGKINKKQlVKAIFVE 631
Cdd:PRK05605 539 KVPRRFYHVDELPRDQLGKVRRRE-VREELLE 569
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
68-626 |
1.52e-54 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 193.92 E-value: 1.52e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 68 EAVAAHDPTSTAVVHalSGRRFTYGELLGDVARARERLREAVDGKPltGERVAFLIENSYDYVVpeahsrdvvMFLAVMA 147
Cdd:PRK06839 9 EKRAYLHPDRIAIIT--EEEEMTYKQLHEYVSKVAAYLIYELNVKK--GERIAILSQNSLEYIV---------LLFAIAK 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 148 ARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYFELPKhtGSSHgaKEAVSLADEDFSGA 227
Cdd:PRK06839 76 VECIAVPLNIRLTENELIFQLKDSGTTVLFVEKTFQNMALSMQKVSYVQRVISITSLK--EIED--RKIDNFVEKNESAS 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 228 GLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAvw 307
Cdd:PRK06839 152 FIICYTSGTTGKPKGAVLTQENMFWNALNNTFAIDLTMHDRSIVLLPLFHIGGIGLFAFPTLFAGGVIIVPRKFEPTK-- 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 308 krfAAPFLPTNgtattapPISFFTVVPTVYSRLLASHKTLSPQMQaatseatspaNLRVNISGSAalPTPVKAAWAALSG 387
Cdd:PRK06839 230 ---ALSMIEKH-------KVTVVMGVPTIHQALINCSKFETTNLQ----------SVRWFYNGGA--PCPEELMREFIDR 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 388 GNVLLERYGMTE----VGMALScglAFADRVDASVGWPLPSVEARLVDMDTGDViEPGHelddhgrerVGEIQLRGPTVF 463
Cdd:PRK06839 288 GFLFGQGFGMTEtsptVFMLSE---EDARRKVGSIGKPVLFCDYELIDENKNKV-EVGE---------VGELLIRGPNVM 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 464 REYWRNPDATAkEFVDDGgdgrgrWFKTGDVAiRREPVGAAgrnshrqpwaqgpmyFIQGRKTaDIIKTGGEKVSALEVE 543
Cdd:PRK06839 355 KEYWNRPDATE-ETIQDG------WLCTGDLA-RVDEDGFV---------------YIVGRKK-EMIISGGENIYPLEVE 410
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 544 RELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQ 623
Cdd:PRK06839 411 QVINKLSDVYEVAVVGRQHVKWGEIPIAFIVKKSSSVL--IEKDVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQ 488
|
...
gi 573989775 624 LVK 626
Cdd:PRK06839 489 LVN 491
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
71-624 |
6.96e-54 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 192.45 E-value: 6.96e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARA 150
Cdd:PRK08316 21 ARRYPDKTALVFG--DRSWTYAELDAAVNRVAAALLDLGLKK---GDRVAALGHNSDAYAL---------LWLACARAGA 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 151 IAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYfeLPKHTGSSHGAKE--------------- 215
Cdd:PRK08316 87 VHVPVNFMLTGEELAYILDHSGARAFLVDPALAPTAEAALALLPVDTLIL--SLVLGGREAPGGWldfadwaeagsvaep 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 216 AVSLADEDFSGaglMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSV 295
Cdd:PRK08316 165 DVELADDDLAQ---ILYTSGTESLPKGAMLTHRALIAEYVSCIVAGDMSADDIPLHALPLYHCAQLDVFLGPYLYVGATN 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 296 EFMFPFNADAVWKRFAApflptnGTATtappiSFFtVVPTVYSRLLAShktlsPQMqaATSEATSpanLRVNISGSAALP 375
Cdd:PRK08316 242 VILDAPDPELILRTIEA------ERIT-----SFF-APPTVWISLLRH-----PDF--DTRDLSS---LRKGYYGASIMP 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 376 TPVKaawaalsggNVLLER---------YGMTEVG-MALSCGLAFADRVDASVGWPLPSVEARLVDMDTGDVIePGheld 445
Cdd:PRK08316 300 VEVL---------KELRERlpglrfyncYGQTEIApLATVLGPEEHLRRPGSAGRPVLNVETRVVDDDGNDVA-PG---- 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 446 dhgreRVGEIQLRGPTVFREYWRNPDATAKEFvddggdgRGRWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIQGRK 525
Cdd:PRK08316 366 -----EVGEIVHRSPQLMLGYWDDPEKTAEAF-------RGGWFHSGDLGVMDE---------------EGYITVVDRKK 418
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 526 taDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVM 605
Cdd:PRK08316 419 --DMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATV--TEDELIAHCRARLAGFKVPKRV 494
|
570
....*....|....*....
gi 573989775 606 KIVNHIPRNAMGKINKKQL 624
Cdd:PRK08316 495 IFVDELPRNPSGKILKREL 513
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
68-624 |
2.62e-52 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 187.80 E-value: 2.62e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 68 EAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVPeahsrdvvmFLAVMA 147
Cdd:PRK07656 12 ARAARRFGDKEAYVFG--DQRLTYAELNARVRRAAAALAALGIGK---GDRVAIWAPNSPHWVIA---------ALGALK 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 148 ARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLAT--DLQQKpVYFELPKHTGSSHGAK---EAVSLADE 222
Cdd:PRK07656 78 AGAVVVPLNTRYTADEAAYILARGDAKALFVLGLFLGVDYSATTRlpALEHV-VICETEEDDPHTEKMKtftDFLAAGDP 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 223 DFSGAGL-------MLYTSGTTNRPKGVLLPEpvitAQCRSLLQAW----EYSPADRLLHVLPLHHIHGTINAVLTPLFA 291
Cdd:PRK07656 157 AERAPEVdpddvadILFTSGTTGRPKGAMLTH----RQLLSNAADWaeylGLTEGDRYLAANPFFHVFGYKAGVNAPLMR 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 292 GSSVEFMFPFNADAVWKRFAAPflptngtattapPISFFTVVPTVYSRLLashktlspqmQAATSEATSPANLRVNISGS 371
Cdd:PRK07656 233 GATILPLPVFDPDEVFRLIETE------------RITVLPGPPTMYNSLL----------QHPDRSAEDLSSLRLAVTGA 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 372 AALPTPVKAAWAALSGGNVLLERYGMTEvgmalSCGLAFADRVD-------ASVGWPLPSVEARLVDmdtgdviEPGHEL 444
Cdd:PRK07656 291 ASMPVALLERFESELGVDIVLTGYGLSE-----ASGVTTFNRLDddrktvaGTIGTAIAGVENKIVN-------ELGEEV 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 445 DDHgreRVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaAGrnshrqpwaqgpmYF-IQG 523
Cdd:PRK07656 359 PVG---EVGELLVRGPNVMKGYYDDPEATAAAIDADG------WLHTGDLGRLDE----EG-------------YLyIVD 412
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 524 RKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQ 603
Cdd:PRK07656 413 RKK-DMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGVPDERLGEVGKAYVVLKPGAEL--TEEELIAYCREHLAKYKVPR 489
|
570 580
....*....|....*....|.
gi 573989775 604 VMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK07656 490 SIEFLDELPKNATGKVLKRAL 510
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
86-624 |
1.69e-51 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 183.65 E-value: 1.69e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 86 GRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSydyvvPEAhsrdVVMFLAVMAARAIAVPLSPPFPAPELQ 165
Cdd:cd05934 1 GRRWTYAELLRESARIAAALAALGIRP---GDRVALMLDNC-----PEF----LFAWFALAKLGAVLVPINTALRGDELA 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 166 YILDQSAASVLVASPkfaakarevlatdlqqkpvyfelpkhtgsshgakeavsladedFSgaglMLYTSGTTNRPKGVLL 245
Cdd:cd05934 69 YIIDHSGAQLVVVDP-------------------------------------------AS----ILYTSGTTGPPKGVVI 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 246 PEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAVWKRFAapflpTNGtATtap 325
Cdd:cd05934 102 THANLTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVLLPRFSASRFWSDVR-----RYG-AT--- 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 326 pisFFTVVPTVYSRLLAshktlspqmqaaTSEATSPANLRVNISGSAALPTPVKAAWAAlSGGNVLLERYGMTEVGMALS 405
Cdd:cd05934 173 ---VTNYLGAMLSYLLA------------QPPSPDDRAHRLRAAYGAPNPPELHEEFEE-RFGVRLLEGYGMTETIVGVI 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 406 cGLAFADRVDASVGWPLPSVEARLVDMDtgdviepGHELDDhgrERVGEIQLR---GPTVFREYWRNPDATAKEFvddgg 482
Cdd:cd05934 237 -GPRDEPRRPGSIGRPAPGYEVRIVDDD-------GQELPA---GEPGELVIRglrGWGFFKGYYNMPEATAEAM----- 300
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 483 dgRGRWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIqGRKtADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPS 562
Cdd:cd05934 301 --RNGWFHTGDLGYRDA---------------DGFFYFV-DRK-KDMIRRRGENISSAEVERAILRHPAVREAAVVAVPD 361
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 573989775 563 GNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd05934 362 EVGEDEVKAVVVLRPGETL--DPEELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQL 421
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
88-624 |
1.31e-50 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 181.42 E-value: 1.31e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 88 RFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLSPPFPAPELQYI 167
Cdd:cd05903 1 RLTYSELDTRADRLAAGLAALGVGP---GDVVAFQLPNWWEFAV---------LYLACLRIGAVTNPILPFFREHELAFI 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 168 LDQSAASVLVASPKFAAkarevlaTDLQQKPvyfelpkhtgsshgakEAVSLadedfsgaglMLYTSGTTNRPKGVLLPE 247
Cdd:cd05903 69 LRRAKAKVFVVPERFRQ-------FDPAAMP----------------DAVAL----------LLFTSGTTGEPKGVMHSH 115
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 248 PVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAvwkrfAAPFLPTNGTATTAPPI 327
Cdd:cd05903 116 NTLSASIRQYAERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIWDPDK-----ALALMREHGVTFMMGAT 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 328 SFftvvptvysrllashktLSPQMQAATSEATSPANLRVNISGSAALPTPVkAAWAALSGGNVLLERYGMTEVGMAL-SC 406
Cdd:cd05903 191 PF-----------------LTDLLNAVEEAGEPLSRLRTFVCGGATVPRSL-ARRAAELLGAKVCSAYGSTECPGAVtSI 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 407 GLAFADRVDASVGWPLPSVEARLVDmDTGDVIEPGHElddhgrervGEIQLRGPTVFREYWRNPDATAKEFvDDGgdgrg 486
Cdd:cd05903 253 TPAPEDRRLYTDGRPLPGVEIKVVD-DTGATLAPGVE---------GELLSRGPSVFLGYLDRPDLTADAA-PEG----- 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 487 rWFKTGDVAIrrepvgaagrnshrqpWAQGPMYFIQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWG 566
Cdd:cd05903 317 -WFRTGDLAR----------------LDEDGYLRITGR-SKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLG 378
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 573989775 567 QKVGAVIILDPDHAVkwTPLEMRRAL-KSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd05903 379 ERACAVVVTKSGALL--TFDELVAYLdRQGVAKQYWPERLVHVDDLPRTPSGKVQKFRL 435
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
89-583 |
1.47e-50 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 181.64 E-value: 1.47e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 89 FTYGELLGDVARARERLReAVDGKPltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLSPPFPAPELQYIL 168
Cdd:cd05907 6 ITWAEFAEEVRALAKGLI-ALGVEP--GDRVAILSRNRPEWTI---------ADLAILAIGAVPVPIYPTSSAEQIAYIL 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 169 DQSAASVLVASPKfaakarEVLATdlqqkpvyfelpkhtgsshgakeavsladedfsgaglMLYTSGTTNRPKGVLLPEP 248
Cdd:cd05907 74 NDSEAKALFVEDP------DDLAT-------------------------------------IIYTSGTTGRPKGVMLSHR 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 249 VITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVefMFPFNADAVWKRFAApFLPTngtattappis 328
Cdd:cd05907 111 NILSNALALAERLPATEGDRHLSFLPLAHVFERRAGLYVPLLAGARI--YFASSAETLLDDLSE-VRPT----------- 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 329 FFTVVPTVYSRL-LASHKTLSPQMQAATSEATSPANLRVNISGSAALPTPVKAAWAALsgGNVLLERYGMTEVGMALSCG 407
Cdd:cd05907 177 VFLAVPRVWEKVyAAIKVKAVPGLKRKLFDLAVGGRLRFAASGGAPLPAELLHFFRAL--GIPVYEGYGLTETSAVVTLN 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 408 LAFADRVDaSVGWPLPSVEARLVDmdtgdviepghelddhgrerVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgr 487
Cdd:cd05907 255 PPGDNRIG-TVGKPLPGVEVRIAD--------------------DGEILVRGPNVMLGYYKNPEATAEALDADG------ 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 488 WFKTGDVAiRREPVGaagrnshrqpwaqgpMYFIQGRKTaDIIKT-GGEKVSALEVERELLSLP---QVAEAavvavpsg 563
Cdd:cd05907 308 WLHTGDLG-EIDEDG---------------FLHITGRKK-DLIITsGGKNISPEPIENALKASPlisQAVVI-------- 362
|
490 500
....*....|....*....|...
gi 573989775 564 nwGQK---VGAVIILDPDHAVKW 583
Cdd:cd05907 363 --GDGrpfLVALIVPDPEALEAW 383
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
75-624 |
4.93e-50 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 181.16 E-value: 4.93e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHALSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVP 154
Cdd:PRK09088 9 PQRLAAVDLALGRRWTYAELDALVGRLAAVLRRRGCVD---GERLAVLARNSVWLVA---------LHFACARVGAIYVP 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 155 LSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYFELPKHTGSShgAKEAVSLadedfsgaglMLYTS 234
Cdd:PRK09088 77 LNWRLSASELDALLQDAEPRLLLGDDAVAAGRTDVEDLAAFIASADALEPADTPSI--PPERVSL----------ILFTS 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 235 GTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAVWKRFAAPF 314
Cdd:PRK09088 145 GTSGQPKGVMLSERNLQQTAHNFGVLGRVDAHSSFLCDAPMFHIIGLITSVRPVLAVGGSILVSNGFEPKRTLGRLGDPA 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 315 LPTngtattappisfftvvptvysrllaSHKTLSPQMQAA-----TSEATSPANLRVNISGSAALPTPVKAAWaaLSGGN 389
Cdd:PRK09088 225 LGI-------------------------THYFCVPQMAQAfraqpGFDAAALRHLTALFTGGAPHAAEDILGW--LDDGI 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 390 VLLERYGMTE----VGMALSCGLAFADRvdASVGWPLPSVEARLVDmDTGDVIEPGHElddhgrervGEIQLRGPTVFRE 465
Cdd:PRK09088 278 PMVDGFGMSEagtvFGMSVDCDVIRAKA--GAAGIPTPTVQTRVVD-DQGNDCPAGVP---------GELLLRGPNLSPG 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 466 YWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRRepvgaagrnshrqpwAQGPMYFIQGRKtaDIIKTGGEKVSALEVERE 545
Cdd:PRK09088 346 YWRRPQATARAFTGDG------WFRTGDIARRD---------------ADGFFWVVDRKK--DMFISGGENVYPAEIEAV 402
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 573989775 546 LLSLPQVAEAAVVAVPSGNWGQkVGAVIILdPDHAVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK09088 403 LADHPGIRECAVVGMADAQWGE-VGYLAIV-PADGAPLDLERIRSHLSTRLAKYKVPKHLRLVDALPRTASGKLQKARL 479
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
88-624 |
8.79e-49 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 176.13 E-value: 8.79e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 88 RFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVPeahsrdvvmFLAVMAARAIAVPLSPPFPAPELQYI 167
Cdd:cd05935 1 SLTYLELLEVVKKLASFLSNKGVRK---GDRVGICLQNSPQYVIA---------YFAIWRANAVVVPINPMLKERELEYI 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 168 LDQSAASVLVaspkfaakarevlatdlqqkpvyfelpkhTGSSHgakeavsladEDFSgagLMLYTSGTTNRPKGVLLPE 247
Cdd:cd05935 69 LNDSGAKVAV-----------------------------VGSEL----------DDLA---LIPYTSGTTGLPKGCMHTH 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 248 PVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMfpfnadAVWKRFAAPFLPTNGTATtappi 327
Cdd:cd05935 107 FSAAANALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLM------ARWDRETALELIEKYKVT----- 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 328 sFFTVVPTVYSRLLASHKTlspqmqaatsEATSPANLRVNISGSAALPTPVKAAWAALSGGNvLLERYGMTEVgMALSCG 407
Cdd:cd05935 176 -FWTNIPTMLVDLLATPEF----------KTRDLSSLKVLTGGGAPMPPAVAEKLLKLTGLR-FVEGYGLTET-MSQTHT 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 408 LAFADRVDASVGWPLPSVEARLVDMDTGDVIEPGhelddhgreRVGEIQLRGPTVFREYWRNPDATAKEFVDDGGDgrgR 487
Cdd:cd05935 243 NPPLRPKLQCLGIP*FGVDARVIDIETGRELPPN---------EVGEIVVRGPQIFKGYWNRPEETEESFIEIKGR---R 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 488 WFKTGDVAIRREpvgaagrnshrqpwaQGpMYFIQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQ 567
Cdd:cd05935 311 FFRTGDLGYMDE---------------EG-YFFFVDR-VKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGE 373
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 573989775 568 KVGAVIILDPDHAVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd05935 374 EVKAFIVLRPEYRGKVTEEDIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
61-583 |
6.67e-47 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 174.52 E-value: 6.67e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 61 LPRLpiFEAVAAHDPTSTAVVHALSG--RRFTYGELLGDVARARERLREAvdG-KPltGERVAFLIENSYDYVVpeahsr 137
Cdd:COG1022 13 LPDL--LRRRAARFPDRVALREKEDGiwQSLTWAEFAERVRALAAGLLAL--GvKP--GDRVAILSDNRPEWVI------ 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 138 dvvMFLAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVAS-PKFAAKAREVLA--TDLQqKPVYFElpkhTGSSHGAK 214
Cdd:COG1022 81 ---ADLAILAAGAVTVPIYPTSSAEEVAYILNDSGAKVLFVEdQEQLDKLLEVRDelPSLR-HIVVLD----PRGLRDDP 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 215 EAVSLAD-----EDFSGAGL---------------MLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLP 274
Cdd:COG1022 153 RLLSLDEllalgREVADPAElearraavkpddlatIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLP 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 275 LHHIHGTINAVLTpLFAGSSVEFmfPFNADAVWKRFAApFLPTngtattappisFFTVVP----TVYSRLLASHKTLSPQ 350
Cdd:COG1022 233 LAHVFERTVSYYA-LAAGATVAF--AESPDTLAEDLRE-VKPT-----------FMLAVPrvweKVYAGIQAKAEEAGGL 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 351 MQA------ATSEATSPA--------------------------------NLRVNISGSAALPTPVKAAWAALsgGNVLL 392
Cdd:COG1022 298 KRKlfrwalAVGRRYARArlagkspslllrlkhaladklvfsklrealggRLRFAVSGGAALGPELARFFRAL--GIPVL 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 393 ERYGMTEvgmalSCGLAFADRVDA----SVGWPLPSVEARLVDmdtgdviepgheldDhgrervGEIQLRGPTVFREYWR 468
Cdd:COG1022 376 EGYGLTE-----TSPVITVNRPGDnrigTVGPPLPGVEVKIAE--------------D------GEILVRGPNVMKGYYK 430
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 469 NPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaAGRnshrqpwaqgpmYFIQGRKtADIIKT-GGEKVSALEVERELL 547
Cdd:COG1022 431 NPEATAEAFDADG------WLHTGDIGELDE----DGF------------LRITGRK-KDLIVTsGGKNVAPQPIENALK 487
|
570 580 590 600
....*....|....*....|....*....|....*....|....*.
gi 573989775 548 SLP---------QvaeaavvavpsgnwGQK-VGAVIILDPDHAVKW 583
Cdd:COG1022 488 ASPlieqavvvgD--------------GRPfLAALIVPDFEALGEW 519
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
61-624 |
7.31e-45 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 167.83 E-value: 7.31e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 61 LPRLPIFE--AVAAHD-PTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKPltGERVAFLIENSYDYVVpeAHsr 137
Cdd:PRK08314 7 LPETSLFHnlEVSARRyPDKTAIVFY--GRAISYRELLEEAERLAGYLQQECGVRK--GDRVLLYMQNSPQFVI--AY-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 138 dvvmfLAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLA-TDLQQKPVYF---ELPKHTGSSHGA 213
Cdd:PRK08314 79 -----YAILRANAVVVPVNPMNREEELAHYVTDSGARVAIVGSELAPKVAPAVGnLRLRHVIVAQysdYLPAEPEIAVPA 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 214 KEAVSLADEDFSGAG-------------------------LMLYTSGTTNRPKGVLLPEPviTAQCRSLLQA-WEYSPAD 267
Cdd:PRK08314 154 WLRAEPPLQALAPGGvvawkealaaglappphtagpddlaVLPYTSGTTGVPKGCMHTHR--TVMANAVGSVlWSNSTPE 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 268 -RLLHVLPLHHIHGTINAVLTPLFAGSSVEFMfpfnadAVWKRFAApflptnGTATTAPPISFFTVVPTVYSRLLAShkt 346
Cdd:PRK08314 232 sVVLAVLPLFHVTGMVHSMNAPIYAGATVVLM------PRWDREAA------ARLIERYRVTHWTNIPTMVVDFLAS--- 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 347 lsPQMQAAtseatSPANLRVNISGSAALPTPVkaawaalsgGNVLLERYGMTEV-GMALSCGLAF-----ADRVDAS-VG 419
Cdd:PRK08314 297 --PGLAER-----DLSSLRYIGGGGAAMPEAV---------AERLKELTGLDYVeGYGLTETMAQthsnpPDRPKLQcLG 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 420 WPLPSVEARLVDMDTGDVIEPGhelddhgreRVGEIQLRGPTVFREYWRNPDATAKEFVDDGGDgrgRWFKTGDVAIRRE 499
Cdd:PRK08314 361 IPTFGVDARVIDPETLEELPPG---------EVGEIVVHGPQVFKGYWNRPEATAEAFIEIDGK---RFFRTGDLGRMDE 428
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 500 pvgaagrnshrqpwaQGpmYFIQGRKTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDH 579
Cdd:PRK08314 429 ---------------EG--YFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQEACVIATPDPRRGETVKAVVVLRPEA 491
|
570 580 590 600
....*....|....*....|....*....|....*....|....*
gi 573989775 580 AVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK08314 492 RGKTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKILWRQL 536
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
87-624 |
1.01e-44 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 166.78 E-value: 1.01e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 87 RRFTYGELLGDVARARERLREaVDGKPltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLSPPFPAPELQY 166
Cdd:cd05959 28 GSLTYAELEAEARRVAGALRA-LGVKR--EERVLLIMLDTVDFPT---------AFLGAIRAGIVPVPVNTLLTPDDYAY 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 167 ILDQSAASVLVASPKFAAKAREVLA---TDLQQKPVYFELPKHTGSSHGAKEAVSLADEDFSGA------GLMLYTSGTT 237
Cdd:cd05959 96 YLEDSRARVVVVSGELAPVLAAALTkseHTLVVLIVSGGAGPEAGALLLAELVAAEAEQLKPAAthaddpAFWLYSSGST 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 238 NRPKGVLLPEPVITAQCRSLLQAWEY-SPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFM--FPfNADAVWKRfaapf 314
Cdd:cd05959 176 GRPKGVVHLHADIYWTAELYARNVLGiREDDVCFSAAKLFFAYGLGNSLTFPLSVGATTVLMpeRP-TPAAVFKR----- 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 315 lptngtATTAPPISFFTVvPTVYSRLLAShktlsPQMQAatseaTSPANLRVNISGSAALPTPVKAAWAALSGGNVLlER 394
Cdd:cd05959 250 ------IRRYRPTVFFGV-PTLYAAMLAA-----PNLPS-----RDLSSLRLCVSAGEALPAEVGERWKARFGLDIL-DG 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 395 YGMTEVG-MALScglAFADRVD-ASVGWPLPSVEARLVDMDTGDViepghelddhGRERVGEIQLRGPTVFREYWRNPDA 472
Cdd:cd05959 312 IGSTEMLhIFLS---NRPGRVRyGTTGKPVPGYEVELRDEDGGDV----------ADGEPGELYVRGPSSATMYWNNRDK 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 473 TAKEFVddggdgrGRWFKTGDVAIRREPvgaaGRnshrqpwaqgpmYFIQGRkTADIIKTGGEKVSALEVERELLSLPQV 552
Cdd:cd05959 379 TRDTFQ-------GEWTRTGDKYVRDDD----GF------------YTYAGR-ADDMLKVSGIWVSPFEVESALVQHPAV 434
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 573989775 553 AEAAVVAVPSGNWGQKVGAVIILDPD-HAVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd05959 435 LEAAVVGVEDEDGLTKPKAFVVLRPGyEDSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKL 507
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
71-636 |
1.59e-44 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 167.21 E-value: 1.59e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHA---LSGRRFTYGELLGDVARARERLREAvdG-KPltGERVAFLIENsydyvVPEAhsrdVVMFLAVM 146
Cdd:COG0365 19 AEGRGDKVALIWEgedGEERTLTYAELRREVNRFANALRAL--GvKK--GDRVAIYLPN-----IPEA----VIAMLACA 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 147 AARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFA---------AKAREVLA--TDLQQKPVYfelpKHTGSSHGAKE 215
Cdd:COG0365 86 RIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLrggkvidlkEKVDEALEelPSLEHVIVV----GRTGADVPMEG 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 216 AVSLADE-------------DFSGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAW-EYSPADRLLHVLPLHHIHGT 281
Cdd:COG0365 162 DLDWDELlaaasaefepeptDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVlDLKPGDVFWCTADIGWATGH 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 282 INAVLTPLFAGSSVeFMF-----PFNADAVWkRFAAPFLPTNgtattappisFFTVvPTVYSRLlashktlspqMQAATS 356
Cdd:COG0365 242 SYIVYGPLLNGATV-VLYegrpdFPDPGRLW-ELIEKYGVTV----------FFTA-PTAIRAL----------MKAGDE 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 357 EATSP--ANLRVNIS-GSAALPTPVKAAWAALsgGNVLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDmD 433
Cdd:COG0365 299 PLKKYdlSSLRLLGSaGEPLNPEVWEWWYEAV--GVPIVDGWGQTETGGIFISNLPGLPVKPGSMGKPVPGYDVAVVD-E 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 434 TGDVIEPGhelddhgreRVGEIQLRG--PTVFREYWRNPDATAKEFVDDGGDgrgrWFKTGDVAIRREpvgaagrnshrq 511
Cdd:COG0365 376 DGNPVPPG---------EEGELVIKGpwPGMFRGYWNDPERYRETYFGRFPG----WYRTGDGARRDE------------ 430
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 512 pwaQGpMYFIQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHA-----VKwtpl 586
Cdd:COG0365 431 ---DG-YFWILGR-SDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQVVKAFVVLKPGVEpsdelAK---- 501
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|
gi 573989775 587 EMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKInKKQLVKAIFVEETSGD 636
Cdd:COG0365 502 ELQAHVREELGPYAYPREIEFVDELPKTRSGKI-MRRLLRKIAEGRPLGD 550
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
67-624 |
4.70e-44 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 164.72 E-value: 4.70e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 67 FEAVAAHdPTSTAVVHALSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVM 146
Cdd:cd05904 12 FLFASAH-PSRPALIDAATGRALTYAELERRVRRLAAGLAKRGGRK---GDVVLLLSPNSIEFPV---------AFLAVL 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 147 AARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKARE-----VLATDLQQKPVYFELPKHTGSSHGAKEAVSLAD 221
Cdd:cd05904 79 SLGAVVTTANPLSTPAEIAKQVKDSGAKLAFTTAELAEKLASlalpvVLLDSAEFDSLSFSDLLFEADEAEPPVVVIKQD 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 222 EdfsgAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAW--EYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMF 299
Cdd:cd05904 159 D----VAALLYSSGTTGRSKGVMLTHRNLIAMVAQFVAGEgsNSDSEDVFLCVLPMFHIYGLSSFALGLLRLGATVVVMP 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 300 PFNADAVWK---RFAAPFLPTngtattAPPIsfftVVPTVYSRLLASHKTLSpqmqaatseatspanLRVNISGSAALPT 376
Cdd:cd05904 235 RFDLEELLAaieRYKVTHLPV------VPPI----VLALVKSPIVDKYDLSS---------------LRQIMSGAAPLGK 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 377 PVKAAWAALSGGNVLLERYGMTEVGMALSCGLAFADRVDA--SVGWPLPSVEARLVDMDTGDVIEPGhelddhgreRVGE 454
Cdd:cd05904 290 ELIEAFRAKFPNVDLGQGYGMTESTGVVAMCFAPEKDRAKygSVGRLVPNVEAKIVDPETGESLPPN---------QTGE 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 455 IQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAirrepvgaagrnshrqpwaqgpmY-------FIQGR-Kt 526
Cdd:cd05904 361 LWIRGPSIMKGYLNNPEATAATIDKEG------WLHTGDLC-----------------------YidedgylFIVDRlK- 410
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 527 aDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKipQVMK 606
Cdd:cd05904 411 -ELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRKPGSSL--TEDEIMDFVAKQVAPYK--KVRK 485
|
570 580
....*....|....*....|
gi 573989775 607 I--VNHIPRNAMGKINKKQL 624
Cdd:cd05904 486 VafVDAIPKSPSGKILRKEL 505
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
75-624 |
2.38e-43 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 161.54 E-value: 2.38e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVP 154
Cdd:cd05930 1 PDAVAVVDG--DQSLTYAELDARANRLARYLRERGVGP---GDLVAVLLERSLEMVV---------AILAVLKAGAAYVP 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 155 LSPPFPAPELQYILDQSAASVLVASPkfaakarevlatdlqQKPVYfelpkhtgsshgakeavsladedfsgaglMLYTS 234
Cdd:cd05930 67 LDPSYPAERLAYILEDSGAKLVLTDP---------------DDLAY-----------------------------VIYTS 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 235 GTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIhGTINAVLTPLFAGSSV---EFMFPFNADAVWKRFA 311
Cdd:cd05930 103 GSTGKPKGVMVEHRGLVNLLLWMQEAYPLTPGDRVLQFTSFSFD-VSVWEIFGALLAGATLvvlPEEVRKDPEALADLLA 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 312 APflptngtattapPISFFTVVPTVYSRLLAshktlspqmqaaTSEATSPANLRVNISGSAALPTPVKAAWAALSGGNVL 391
Cdd:cd05930 182 EE------------GITVLHLTPSLLRLLLQ------------ELELAALPSLRLVLVGGEALPPDLVRRWRELLPGARL 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 392 LERYGMTEV-GMALSCGLAFADRVDASV--GWPLPSVEARLVDmDTGDVIEPGHelddhgrerVGEIQLRGPTVFREYWR 468
Cdd:cd05930 238 VNLYGPTEAtVDATYYRVPPDDEEDGRVpiGRPIPNTRVYVLD-ENLRPVPPGV---------PGELYIGGAGLARGYLN 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 469 NPDATAKEFVDDGGDGRGRWFKTGDVAiRREPVGAagrnshrqpwaqgpMYFIqGRKtADIIKTGGEKVSALEVERELLS 548
Cdd:cd05930 308 RPELTAERFVPNPFGPGERMYRTGDLV-RWLPDGN--------------LEFL-GRI-DDQVKIRGYRIELGEIEAALLA 370
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 573989775 549 LPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd05930 371 HPGVREAAVVAREDGDGEKRLVAYVVPDEGGEL--DEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
90-550 |
2.96e-43 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 160.51 E-value: 2.96e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 90 TYGELLGDVARARERLREAVDGKPltGERVAFLIENSYDyvvpeahsrDVVMFLAVMAARAIAVPLSPPFPAPELQYILD 169
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAGGVGP--GDRVAVLLERSAE---------LVVAILAVLKAGAAYVPLDPAYPAERLAFILE 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 170 QSAASVLVASPKFAAKAREVLATDLqqkpvyfELPKHTGSSHGAKEAVSLADEDFSGAGLM--LYTSGTTNRPKGVLLPE 247
Cdd:TIGR01733 70 DAGARLLLTDSALASRLAGLVLPVI-------LLDPLELAALDDAPAPPPPDAPSGPDDLAyvIYTSGSTGRPKGVVVTH 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 248 PVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTInAVLTPLFAGSSVEFMfPFNADAVWKRFAAPFLPTNgtattapPI 327
Cdd:TIGR01733 143 RSLVNLLAWLARRYGLDPDDRVLQFASLSFDASVE-EIFGALLAGATLVVP-PEDEERDDAALLAALIAEH-------PV 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 328 SFFTVVPTVYSRLLASHktlspqmqaatseATSPANLRVNISGSAALPTPVKAAWAALSGGNVLLERYGMTE----VGMA 403
Cdd:TIGR01733 214 TVLNLTPSLLALLAAAL-------------PPALASLRLVILGGEALTPALVDRWRARGPGARLINLYGPTEttvwSTAT 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 404 LSCGLAFADRVDASVGWPLPSVEARLVDMDtgdviepgheLDDHGRERVGEIQLRGPTVFREYWRNPDATAKEFVDD--G 481
Cdd:TIGR01733 281 LVDPDDAPRESPVPIGRPLANTRLYVLDDD----------LRPVPVGVVGELYIGGPGVARGYLNRPELTAERFVPDpfA 350
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 573989775 482 GDGRGRWFKTGDVAiRREPVGA---AGRNSHRqpwaqgpmyfiqgrktadiIKTGGEKVSALEVERELLSLP 550
Cdd:TIGR01733 351 GGDGARLYRTGDLV-RYLPDGNlefLGRIDDQ-------------------VKIRGYRIELGEIEAALLRHP 402
|
|
| menE |
TIGR01923 |
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ... |
90-624 |
4.44e-43 |
|
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]
Pssm-ID: 162605 [Multi-domain] Cd Length: 436 Bit Score: 160.69 E-value: 4.44e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 90 TYGELLGDVARARERLREAVDGKpltGERVAFLIENSydyvvpeAHSrdVVMFLAVMAARAIAVPLSPPFPAPELQYILD 169
Cdd:TIGR01923 1 TWQDLDCEAAHLAKALKAQGIRS---GSRVALVGQNS-------IEM--VLLLHACLLLGAEIAMLNTRLTENERTNQLE 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 170 QSAASVLVASPKFAAKarEVLATDLQQKPVYFELPKHTGSSHGAKEAVSLadedfsgaglmLYTSGTTNRPKGVLLPEPV 249
Cdd:TIGR01923 69 DLDVQLLLTDSLLEEK--DFQADSLDRIEAAGRYETSLSASFNMDQIATL-----------MFTSGTTGKPKAVPHTFRN 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 250 ITAQCRSLLQAWEYSPADRLLHVLPLHHIHGtINAVLTPLFAGSSVEFMFPFNADAvwkrfaapflptngTATTAPPISF 329
Cdd:TIGR01923 136 HYASAVGSKENLGFTEDDNWLLSLPLYHISG-LSILFRWLIEGATLRIVDKFNQLL--------------EMIANERVTH 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 330 FTVVPTVYSRLLashktlspqmqaatSEATSPANLRVNISGSAALPTPVkaAWAALSGGNVLLERYGMTEvgmALSCGLA 409
Cdd:TIGR01923 201 ISLVPTQLNRLL--------------DEGGHNENLRKILLGGSAIPAPL--IEEAQQYGLPIYLSYGMTE---TCSQVTT 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 410 FADRVDA---SVGWPLPSVEARL-VDmdtgdviepghelddhGRERVGEIQLRGPTVFREYWRNPDATakEFVDDGGdgr 485
Cdd:TIGR01923 262 ATPEMLHarpDVGRPLAGREIKIkVD----------------NKEGHGEIMVKGANLMKGYLYQGELT--PAFEQQG--- 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 486 grWFKTGDVairrepvgaaGRNSHrqpwaQGPMYfIQGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNW 565
Cdd:TIGR01923 321 --WFNTGDI----------GELDG-----EGFLY-VLGRRD-DLIISGGENIYPEEIETVLYQHPGIQEAVVVPKPDAEW 381
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 573989775 566 GQKVGAVIILDPDhavkWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:TIGR01923 382 GQVPVAYIVSESD----ISQAKLIAYLTEKLAKYKVPIAFEKLDELPYNASGKILRNQL 436
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
75-629 |
1.60e-42 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 160.13 E-value: 1.60e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSydyvvpeahsrdVVMFLAVMAAR---AI 151
Cdd:PRK03640 16 PDRTAIEFE--EKKVTFMELHEAVVSVAGKLAALGVKK---GDRVALLMKNG------------MEMILVIHALQqlgAV 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 152 AVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQqkpvyfELPKhtgsshGAKEAVSLADE-DFSGAGLM 230
Cdd:PRK03640 79 AVLLNTRLSREELLWQLDDAEVKCLITDDDFEAKLIPGISVKFA------ELMN------GPKEEAEIQEEfDLDEVATI 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 231 LYTSGTTNRPKGVLlpepvitaqcrsllQAWE--YSPA------------DRLLHVLPLHHIHGtINAVLTPLFAGSSVE 296
Cdd:PRK03640 147 MYTSGTTGKPKGVI--------------QTYGnhWWSAvgsalnlgltedDCWLAAVPIFHISG-LSILMRSVIYGMRVV 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 297 FMFPFNADAVWKrfaapfLPTNGTATTappISfftVVPTVYSRLLAshktlspqmqaATSEATSPANLRVNISGSAalPT 376
Cdd:PRK03640 212 LVEKFDAEKINK------LLQTGGVTI---IS---VVSTMLQRLLE-----------RLGEGTYPSSFRCMLLGGG--PA 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 377 PVKAAWAALSGGNVLLERYGMTEVGMALsCGLAFADRVD--ASVGWPLPSVEARLVDmdtGDVIEPGHElddhgrerVGE 454
Cdd:PRK03640 267 PKPLLEQCKEKGIPVYQSYGMTETASQI-VTLSPEDALTklGSAGKPLFPCELKIEK---DGVVVPPFE--------EGE 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 455 IQLRGPTVFREYWRNPDATAKEFVDDggdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYfIQGRKTaDIIKTGG 534
Cdd:PRK03640 335 IVVKGPNVTKGYLNREDATRETFQDG-------WFKTGDIGYLDE---------------EGFLY-VLDRRS-DLIISGG 390
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 535 EKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDpdhaVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRN 614
Cdd:PRK03640 391 ENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPVAFVVKS----GEVTEEELRHFCEEKLAKYKVPKRFYFVEELPRN 466
|
570
....*....|....*
gi 573989775 615 AMGKINKKQLVKAIF 629
Cdd:PRK03640 467 ASGKLLRHELKQLVE 481
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
83-624 |
4.93e-42 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 159.38 E-value: 4.93e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 83 ALSGRRFTYGELLGDVARArERLREAVDGKPltGERVAFLIENsydyvVPEAhsrdVVMFLAVMAARAIAVPLSPPFPAP 162
Cdd:PRK06188 32 VLGDTRLTYGQLADRISRY-IQAFEALGLGT--GDAVALLSLN-----RPEV----LMAIGAAQLAGLRRTALHPLGSLD 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 163 ELQYILDQSAASVLVASP-KFAAKAREVLATDLQQKPVYFELPKHTGSSHGAK----EAVSLADEDFSG-AGLMLYTSGT 236
Cdd:PRK06188 100 DHAYVLEDAGISTLIVDPaPFVERALALLARVPSLKHVLTLGPVPDGVDLLAAaakfGPAPLVAAALPPdIAGLAYTGGT 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 237 TNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTInaVLTPLFAGSSVEFMFPFNADAVWKRFAAPflp 316
Cdd:PRK06188 180 TGKPKGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSHAGGAF--FLPTLLRGGTVIVLAKFDPAEVLRAIEEQ--- 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 317 tngtattapPISFFTVVPTVYSRLlashktlspqMQAATSEATSPANLRVNISGSAALpTPVKAAWAALSGGNVLLERYG 396
Cdd:PRK06188 255 ---------RITATFLVPTMIYAL----------LDHPDLRTRDLSSLETVYYGASPM-SPVRLAEAIERFGPIFAQYYG 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 397 MTEVGMALSCgLAFADRVD------ASVGWPLPSVEARLvdmdtgdviepgheLDDHGRE----RVGEIQLRGPTVFREY 466
Cdd:PRK06188 315 QTEAPMVITY-LRKRDHDPddpkrlTSCGRPTPGLRVAL--------------LDEDGREvaqgEVGEICVRGPLVMDGY 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 467 WRNPDATAKEFvddggdgRGRWFKTGDVAiRREPVGaagrnshrqpwaqgpMYFIQGRKTaDIIKTGGEKVSALEVEREL 546
Cdd:PRK06188 380 WNRPEETAEAF-------RDGWLHTGDVA-REDEDG---------------FYYIVDRKK-DMIVTGGFNVFPREVEDVL 435
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 573989775 547 LSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK06188 436 AEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAAV--DAAELQAHVKERKGSVHAPKQVDFVDSLPLTALGKPDKKAL 511
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
65-626 |
5.88e-42 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 159.18 E-value: 5.88e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 65 PIFEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVdgkPLTGERVAFLIENSYDyvvpeahsrDVVMFLA 144
Cdd:TIGR03098 4 HLLEDAAARLPDATALVHH--DRTLTYAALSERVLALASGLRGLG---LARGERVAIYLDKRLE---------TVTAMFG 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 145 VMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLAT-DLQQKPVYFELPKHTGSSHGAKEAVSLADED 223
Cdd:TIGR03098 70 AALAGGVFVPINPLLKAEQVAHILADCNVRLLVTSSERLDLLHPALPGcHDLRTLIIVGDPAHASEGHPGEEPASWPKLL 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 224 FSGAGL------------MLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGtINAVLTPLFA 291
Cdd:TIGR03098 150 ALGDADpphpvidsdmaaILYTSGSTGRPKGVVLSHRNLVAGAQSVATYLENRPDDRLLAVLPLSFDYG-FNQLTTAFYV 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 292 GSSV---EFMFPfnADAVwkrfaapflptngTATTAPPISFFTVVPTVYSRLlashktlspqMQAATSEATSPANLRVNI 368
Cdd:TIGR03098 229 GATVvlhDYLLP--RDVL-------------KALEKHGITGLAAVPPLWAQL----------AQLDWPESAAPSLRYLTN 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 369 SGSAALPTPVKAAWAALSGGNVLLeRYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDMDtGDVIEPGHElddhg 448
Cdd:TIGR03098 284 SGGAMPRATLSRLRSFLPNARLFL-MYGLTEAFRSTYLPPEEVDRRPDSIGKAIPNAEVLVLRED-GSECAPGEE----- 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 449 rervGEIQLRGPTVFREYWRNPDATAKEFVDDggDGRGRWFKTGDVAIrrepvgAAGRNSHRQpwAQGPMYFIqGRKTaD 528
Cdd:TIGR03098 357 ----GELVHRGALVAMGYWNDPEKTAERFRPL--PPFPGELHLPELAV------WSGDTVRRD--EEGFLYFV-GRRD-E 420
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 529 IIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVgAVIILDPDHaVKWTPLEMRRALKSRLAGYKIPQVMKIV 608
Cdd:TIGR03098 421 MIKTSGYRVSPTEVEEVAYATGLVAEAVAFGVPDPTLGQAI-VLVVTPPGG-EELDRAALLAECRARLPNYMVPALIHVR 498
|
570
....*....|....*...
gi 573989775 609 NHIPRNAMGKINKKQLVK 626
Cdd:TIGR03098 499 QALPRNANGKIDRKALAK 516
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
86-627 |
7.33e-41 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 156.48 E-value: 7.33e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 86 GRRFTYGELLGDVARARERL-REAVDgkplTGERVAFLIENSYDYVvpEAhsrdvvmFLAVMAARAIAVPLSPPFPAPEL 164
Cdd:PRK07786 40 GNTTTWRELDDRVAALAGALsRRGVG----FGDRVLILMLNRTEFV--ES-------VLAANMLGAIAVPVNFRLTPPEI 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 165 QYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYFELPKHTGSSHGAKEAVSLADEDFS-------GAGLMLYTSGTT 237
Cdd:PRK07786 107 AFLVSDCGAHVVVTEAALAPVATAVRDIVPLLSTVVVAGGSSDDSVLGYEDLLAEAGPAHApvdipndSPALIMYTSGTT 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 238 NRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHV-LPLHHIHGtINAVLTPLFAGSSVeFMFPFNAdavwkrfaapFLP 316
Cdd:PRK07786 187 GRPKGAVLTHANLTGQAMTCLRTNGADINSDVGFVgVPLFHIAG-IGSMLPGLLLGAPT-VIYPLGA----------FDP 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 317 TNG-TATTAPPISFFTVVPTvysrllashktlspQMQAATSEATS-PANLRVNISGSAALPTP--VKAAWAALSGGNVLL 392
Cdd:PRK07786 255 GQLlDVLEAEKVTGIFLVPA--------------QWQAVCAEQQArPRDLALRVLSWGAAPASdtLLRQMAATFPEAQIL 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 393 ERYGMTEVGmALSCGLAFAD--RVDASVGWPLPSVEARLVDMDTGDViEPGhelddhgreRVGEIQLRGPTVFREYWRNP 470
Cdd:PRK07786 321 AAFGQTEMS-PVTCMLLGEDaiRKLGSVGKVIPTVAARVVDENMNDV-PVG---------EVGEIVYRAPTLMSGYWNNP 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 471 DATAKEFvdDGGdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIQGRKtaDIIKTGGEKVSALEVERELLSLP 550
Cdd:PRK07786 390 EATAEAF--AGG-----WFHSGDLVRQDE---------------EGYVWVVDRKK--DMIISGGENIYCAEVENVLASHP 445
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 573989775 551 QVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLVKA 627
Cdd:PRK07786 446 DIVEVAVIGRADEKWGEVPVAVAAVRNDDAA-LTLEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRER 521
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
75-624 |
1.08e-40 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 154.76 E-value: 1.08e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHalSGRRFTYGELLGDVARARERLREAvDGKPltGERVAFLIENSydyvvpeahSRDVVMFLAVMAARAIAVP 154
Cdd:cd12116 1 PDATAVRD--DDRSLSYAELDERANRLAARLRAR-GVGP--GDRVAVYLPRS---------ARLVAAMLAVLKAGAAYVP 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 155 LSPPFPAPELQYILDQSAASVLVASPKFAAKArevlatdlqqkPVYFELPKHTGSSHGAKEAVSLADEDFSGAGLMLYTS 234
Cdd:cd12116 67 LDPDYPADRLRYILEDAEPALVLTDDALPDRL-----------PAGLPVLLLALAAAAAAPAAPRTPVSPDDLAYVIYTS 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 235 GTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLhvlplhhihgtinAVLTPLFAGSSVEFMFPfnadavwkrfaapf 314
Cdd:cd12116 136 GSTGRPKGVVVSHRNLVNFLHSMRERLGLGPGDRLL-------------AVTTYAFDISLLELLLP-------------- 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 315 LPTNGTATTAPPISffTVVPTVYSRLLASHK-TLspqMQA--------ATSEATSPANLRVnISGSAALPTPVKAAWAAL 385
Cdd:cd12116 189 LLAGARVVIAPRET--QRDPEALARLIEAHSiTV---MQAtpatwrmlLDAGWQGRAGLTA-LCGGEALPPDLAARLLSR 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 386 SGgnVLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDmDTGDVIEPGhelddhgreRVGEIQLRGPTVFRE 465
Cdd:cd12116 263 VG--SLWNLYGPTETTIWSTAARVTAAAGPIPIGRPLANTQVYVLD-AALRPVPPG---------VPGELYIGGDGVAQG 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 466 YWRNPDATAKEFVDD-GGDGRGRWFKTGDVAIRRepvgaagrnshrqpwAQGPMYFIqGRKTaDIIKTGGEKVSALEVER 544
Cdd:cd12116 331 YLGRPALTAERFVPDpFAGPGSRLYRTGDLVRRR---------------ADGRLEYL-GRAD-GQVKIRGHRIELGEIEA 393
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 545 ELLSLPQVAEAAVVAVPSGNWGQKVGAVIildPDHAVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd12116 394 ALAAHPGVAQAAVVVREDGGDRRLVAYVV---LKAGAAPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
78-624 |
2.53e-40 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 153.00 E-value: 2.53e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 78 TAVVHAlsGRRFTYGELLGDVARARERLREAvdGKPlTGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLSP 157
Cdd:cd05919 2 TAFYAA--DRSVTYGQLHDGANRLGSALRNL--GVS-SGDRVLLLMLDSPELVQ---------LFLGCLARGAIAVVINP 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 158 PFPAPELQYILDQSAASVLVASpkfaakarevlatdlqqkpvyfelpkhtgsshgakeavslaDEDfsgAGLMLYTSGTT 237
Cdd:cd05919 68 LLHPDDYAYIARDCEARLVVTS-----------------------------------------ADD---IAYLLYSSGTT 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 238 NRPKGVL--LPEPVITAQ--CRSLLQaweYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFM-FPFNADAVWKRFAA 312
Cdd:cd05919 104 GPPKGVMhaHRDPLLFADamAREALG---LTPGDRVFSSAKMFFGYGLGNSLWFPLAVGASAVLNpGWPTAERVLATLAR 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 313 pFLPTngtattappisFFTVVPTVYSRLLAShktlspqmqaATSEATSPANLRVNISGSAALPTPVKAAWAALSGGNVLl 392
Cdd:cd05919 181 -FRPT-----------VLYGVPTFYANLLDS----------CAGSPDALRSLRLCVSAGEALPRGLGERWMEHFGGPIL- 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 393 ERYGMTEVGMALSCGLAFADRVDaSVGWPLPSVEARLVDmDTGDVIEPGhelddhgreRVGEIQLRGPTVFREYWRNPDA 472
Cdd:cd05919 238 DGIGATEVGHIFLSNRPGAWRLG-STGRPVPGYEIRLVD-EEGHTIPPG---------EEGDLLVRGPSAAVGYWNNPEK 306
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 473 TAKEFvddggdgRGRWFKTGDVaIRREPVGAagrnshrqpwaqgpmYFIQGRKTaDIIKTGGEKVSALEVERELLSLPQV 552
Cdd:cd05919 307 SRATF-------NGGWYRTGDK-FCRDADGW---------------YTHAGRAD-DMLKVGGQWVSPVEVESLIIQHPAV 362
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 573989775 553 AEAAVVAVPSGNWGQKVGAVIILDPDHAVKWTPLE-MRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd05919 363 AEAAVVAVPESTGLSRLTAFVVLKSPAAPQESLARdIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKL 435
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
68-626 |
2.86e-40 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 155.19 E-value: 2.86e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 68 EAVAAHDPTSTAVvHALsGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENsydyvVPEAhsrdVVMFLAVMA 147
Cdd:PRK06710 31 EQMASRYPEKKAL-HFL-GKDITFSVFHDKVKRFANYLQKLGVEK---GDRVAIMLPN-----CPQA----VIGYYGTLL 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 148 ARAIAVPLSPPFPAPELQYILDQSAASVL---------VASPKFAAKAREVLATDLQQkpvYFELPKH------------ 206
Cdd:PRK06710 97 AGGIVVQTNPLYTERELEYQLHDSGAKVIlcldlvfprVTNVQSATKIEHVIVTRIAD---FLPFPKNllypfvqkkqsn 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 207 ----TGSSHGAKEAVSLADEDFSGA----------GLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQaWEYS---PADRL 269
Cdd:PRK06710 174 lvvkVSESETIHLWNSVEKEVNTGVevpcdpendlALLQYTGGTTGFPKGVMLTHKNLVSNTLMGVQ-WLYNckeGEEVV 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 270 LHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAVWKrfaapflptngtATTAPPISFFTVVPTVYSRLLAShktlsP 349
Cdd:PRK06710 253 LGVLPFFHVYGMTAVMNLSIMQGYKMVLIPKFDMKMVFE------------AIKKHKVTLFPGAPTIYIALLNS-----P 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 350 QMQAatseaTSPANLRVNISGSAALPTPVKAAWAALSGGNvLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARL 429
Cdd:PRK06710 316 LLKE-----YDISSIRACISGSAPLPVEVQEKFETVTGGK-LVEGYGLTESSPVTHSNFLWEKRVPGSIGVPWPDTEAMI 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 430 VDMDTGDVIEPGhelddhgreRVGEIQLRGPTVFREYWRNPDATAKeFVDDGgdgrgrWFKTGDVAIRREpvgaagrnsh 509
Cdd:PRK06710 390 MSLETGEALPPG---------EIGEIVVKGPQIMKGYWNKPEETAA-VLQDG------WLHTGDVGYMDE---------- 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 510 rqpwaQGpMYFIQGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMR 589
Cdd:PRK06710 444 -----DG-FFYVKDRKK-DMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVVLKEGTEC--SEEELN 514
|
570 580 590
....*....|....*....|....*....|....*..
gi 573989775 590 RALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLVK 626
Cdd:PRK06710 515 QFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLIE 551
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
86-619 |
4.46e-39 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 149.90 E-value: 4.46e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 86 GRRFTYGELLGDVARARERLReavDGKPLTGERVAFLIENSydyvvpeAHSrdVVMFLAVMAARAIAVPLSPPFPAPELQ 165
Cdd:cd05914 5 GEPLTYKDLADNIAKFALLLK---INGVGTGDRVALMGENR-------PEW--GIAFFAIWTYGAIAVPILAEFTADEVH 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 166 YILDQSAASVLVASpkfaakarevlatdlqqkpvyfelpkhtgsshgakeavslaDEDfsGAGLMLYTSGTTNRPKGVLL 245
Cdd:cd05914 73 HILNHSEAKAIFVS-----------------------------------------DED--DVALINYTSGTTGNSKGVML 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 246 PEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAVWKRFAAPFLPTNGTatTAP 325
Cdd:cd05914 110 TYRNIVSNVDGVKEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDKIPSAKIIALAFAQVTPTLGV--PVP 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 326 ----PISFFTVVPTVYSR----LLAShKTLSPQMQAATSEATSPA---NLRVNISGSAALPTPVKAAWAALsgGNVLLER 394
Cdd:cd05914 188 lvieKIFKMDIIPKLTLKkfkfKLAK-KINNRKIRKLAFKKVHEAfggNIKEFVIGGAKINPDVEEFLRTI--GFPYTIG 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 395 YGMTEVGmALSCGLAFADRVDASVGWPLPSVEARLVDMD--TGDviepghelddhgrervGEIQLRGPTVFREYWRNPDA 472
Cdd:cd05914 265 YGMTETA-PIISYSPPNRIRLGSAGKVIDGVEVRIDSPDpaTGE----------------GEIIVRGPNVMKGYYKNPEA 327
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 473 TAKEFVDDGgdgrgrWFKTGDvairrepVGAAGRNSHrqpwaqgpmYFIQGRKTADIIKTGGEKVSALEVERELLSLPQV 552
Cdd:cd05914 328 TAEAFDKDG------WFHTGD-------LGKIDAEGY---------LYIRGRKKEMIVLSSGKNIYPEEIEAKINNMPFV 385
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 553 AEAAVVAVPsgnwgQKVGAVIILDPD-------------HAVKWtplEMRRALKSRLAGYKIPQVMKIVN-HIPRNAMGK 618
Cdd:cd05914 386 LESLVVVQE-----KKLVALAYIDPDfldvkalkqrniiDAIKW---EVRDKVNQKVPNYKKISKVKIVKeEFEKTPKGK 457
|
.
gi 573989775 619 I 619
Cdd:cd05914 458 I 458
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
82-624 |
6.51e-39 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 150.47 E-value: 6.51e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 82 HALSGRRFTYGELlgdVARAReRLREAVDGKPLT-GERVAFLIENsydyvvpeaHSRDVVMFLAVMAARAIAVPLSPPFP 160
Cdd:cd12119 19 HEGEVHRYTYAEV---AERAR-RLANALRRLGVKpGDRVATLAWN---------THRHLELYYAVPGMGAVLHTINPRLF 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 161 APELQYILDQSAASVLVASPKFAAKArEVLATDLQQKPVYFELPkhTGSSHGAKEAVSLAD-EDFSGAG----------- 228
Cdd:cd12119 86 PEQIAYIINHAEDRVVFVDRDFLPLL-EAIAPRLPTVEHVVVMT--DDAAMPEPAGVGVLAyEELLAAEspeydwpdfde 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 ----LMLYTSGTTNRPKGVLLPEPVITAQCRSLLQ--AWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSV---EFMF 299
Cdd:cd12119 163 ntaaAICYTSGTTGNPKGVVYSHRSLVLHAMAALLtdGLGLSESDVVLPVVPMFHVNAWGLPYAAAMVGAKLVlpgPYLD 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 300 PFNADAVWKRfaapflpTNGTATTAppisfftvVPTVYSRLLASHKTLSPQMQAatseatspanLRVNISGSAALPTPVK 379
Cdd:cd12119 243 PASLAELIER-------EGVTFAAG--------VPTVWQGLLDHLEANGRDLSS----------LRRVVIGGSAVPRSLI 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 380 AAWAALsgGNVLLERYGMTEVGMALSCG--------LAFADRVD--ASVGWPLPSVEARLVDMDTGdviepghELDDHGR 449
Cdd:cd12119 298 EAFEER--GVRVIHAWGMTETSPLGTVArppsehsnLSEDEQLAlrAKQGRPVPGVELRIVDDDGR-------ELPWDGK 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 450 ErVGEIQLRGPTVFREYWRNPDATAKEFVDDggdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYfIQGRkTADI 529
Cdd:cd12119 369 A-VGELQVRGPWVTKSYYKNDEESEALTEDG-------WLRTGDVATIDE---------------DGYLT-ITDR-SKDV 423
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 530 IKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVN 609
Cdd:cd12119 424 IKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGATV--TAEELLEFLADKVAKWWLPDDVVFVD 501
|
570
....*....|....*
gi 573989775 610 HIPRNAMGKINKKQL 624
Cdd:cd12119 502 EIPKTSTGKIDKKAL 516
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
71-499 |
8.99e-39 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 150.47 E-value: 8.99e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVH----ALSGRRFTYGELLgdvARARE---RLREAvdGKPltGERVAFLIENSYDYVVPeahsrdvvmFL 143
Cdd:cd05931 3 AAARPDRPAYTFlddeGGREETLTYAELD---RRARAiaaRLQAV--GKP--GDRVLLLAPPGLDFVAA---------FL 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 144 AVMAARAIAVPLSPPFP---APELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYFELPKHTGSSHGAKEAVSLA 220
Cdd:cd05931 67 GCLYAGAIAVPLPPPTPgrhAERLAAILADAGPRVVLTTAAALAAVRAFAASRPAAGTPRLLVVDLLPDTSAADWPPPSP 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 221 DEDfsGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFP 300
Cdd:cd05931 147 DPD--DIAYLQYTSGSTGTPKGVVVTHRNLLANVRQIRRAYGLDPGDVVVSWLPLYHDMGLIGGLLTPLYSGGPSVLMSP 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 301 --FNAD-AVWKRFAAPFlptNGTATTAPPISFftvvptvysRLLAshktlspqmQAATSEATSPANL---RVNISGS--- 371
Cdd:cd05931 225 aaFLRRpLRWLRLISRY---RATISAAPNFAY---------DLCV---------RRVRDEDLEGLDLsswRVALNGAepv 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 372 --------AALPTPVKAAWAALSGGnvllerYGMTEVGMALSCGLAFA----DRVDA---------------------SV 418
Cdd:cd05931 284 rpatlrrfAEAFAPFGFRPEAFRPS------YGLAEATLFVSGGPPGTgpvvLRVDRdalagravavaaddpaarelvSC 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 419 GWPLPSVEARLVDMDTGDVIEPGhelddhgreRVGEIQLRGPTVFREYWRNPDATAKEFVDDGGDGRGRWFKTGDVAIRR 498
Cdd:cd05931 358 GRPLPDQEVRIVDPETGRELPDG---------EVGEIWVRGPSVASGYWGRPEATAETFGALAATDEGGWLRTGDLGFLH 428
|
.
gi 573989775 499 E 499
Cdd:cd05931 429 D 429
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
71-621 |
2.37e-38 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 148.22 E-value: 2.37e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHAlsGRRFTYGELlgdVARAReRLREAVDGKPL-TGERVAFLIENSYDYVvpEAHsrdvvmfLAVMAAR 149
Cdd:cd12118 14 AAVYPDRTSIVYG--DRRYTWRQT---YDRCR-RLASALAALGIsRGDTVAVLAPNTPAMY--ELH-------FGVPMAG 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 150 AIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAkaREVLATdlqQKPVYfelpkhtgsshgakEAVSLADEDFSGAgl 229
Cdd:cd12118 79 AVLNALNTRLDAEEIAFILRHSEAKVLFVDREFEY--EDLLAE---GDPDF--------------EWIPPADEWDPIA-- 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 230 MLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVeFMFPFNADAVWKr 309
Cdd:cd12118 138 LNYTSGTTGRPKGVVYHHRGAYLNALANILEWEMKQHPVYLWTLPMFHCNGWCFPWTVAAVGGTNV-CLRKVDAKAIYD- 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 310 faapflptngtATTAPPISFFTVVPTVYSRLLAshktlspqmqaATSEATSPANLRVNISGSAALPTPVKAAWAALSGGN 389
Cdd:cd12118 216 -----------LIEKHKVTHFCGAPTVLNMLAN-----------APPSDARPLPHRVHVMTAGAPPPAAVLAKMEELGFD 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 390 VLlERYGMTEV-GMALSCglAFADRVDAsvgwpLPSVE-ARL--------VDMDTGDVIEPGHELD-DHGRERVGEIQLR 458
Cdd:cd12118 274 VT-HVYGLTETyGPATVC--AWKPEWDE-----LPTEErARLkarqgvryVGLEEVDVLDPETMKPvPRDGKTIGEIVFR 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 459 GPTVFREYWRNPDATAKEFvddggdgRGRWFKTGDVAIRrEPVGaagrnshrqpwaqgpmyFIQGRKTA-DIIKTGGEKV 537
Cdd:cd12118 346 GNIVMKGYLKNPEATAEAF-------RGGWFHSGDLAVI-HPDG-----------------YIEIKDRSkDIIISGGENI 400
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 538 SALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDhaVKWTPLEMRRALKSRLAGYKIPQVMkIVNHIPRNAMG 617
Cdd:cd12118 401 SSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEG--AKVTEEEIIAFCREHLAGFMVPKTV-VFGELPKTSTG 477
|
....
gi 573989775 618 KINK 621
Cdd:cd12118 478 KIQK 481
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
90-624 |
2.37e-38 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 146.72 E-value: 2.37e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 90 TYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLSPPFPAPELQYILD 169
Cdd:cd05912 3 TFAELFEEVSRLAEHLAALGVRK---GDRVALLSKNSIEMIL---------LIHALWLLGAEAVLLNTRLTPNELAFQLK 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 170 QSAASVlvaspkfaakarEVLATdlqqkpvyfelpkhtgsshgakeavsladedfsgaglMLYTSGTTNRPKGVLLPEPV 249
Cdd:cd05912 71 DSDVKL------------DDIAT-------------------------------------IMYTSGTTGKPKGVQQTFGN 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 250 ITAQCRSLLQAWEYSPADRLLHVLPLHHIHGtINAVLTPLFAGSSVEFMFPFNADAVwkrfaaPFLPTNGTATTappISf 329
Cdd:cd05912 102 HWWSAIGSALNLGLTEDDNWLCALPLFHISG-LSILMRSVIYGMTVYLVDKFDAEQV------LHLINSGKVTI---IS- 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 330 ftVVPTVYSRLLASHKtlspqmqaatseATSPANLRVNISGSAALPTPVKAAwaALSGGNVLLERYGMTEVGmALSCGLA 409
Cdd:cd05912 171 --VVPTMLQRLLEILG------------EGYPNNLRCILLGGGPAPKPLLEQ--CKEKGIPVYQSYGMTETC-SQIVTLS 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 410 FADRVD--ASVGWPLPSVEARLVDmdtgdviepghelDDHGRERVGEIQLRGPTVFREYWRNPDATAKEFVDDggdgrgr 487
Cdd:cd05912 234 PEDALNkiGSAGKPLFPVELKIED-------------DGQPPYEVGEILLKGPNVTKGYLNRPDATEESFENG------- 293
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 488 WFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIQGRKtaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQ 567
Cdd:cd05912 294 WFKTGDIGYLDE---------------EGFLYVLDRRS--DLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQ 356
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 573989775 568 KVGAVIILDPDhavkWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd05912 357 VPVAFVVSERP----ISEEELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHEL 409
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
230-621 |
3.73e-38 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 144.33 E-value: 3.73e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 230 MLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGtINAVLTPLFAGSSVEFMFPFNADAVWKR 309
Cdd:cd17637 5 IIHTAAVAGRPRGAVLSHGNLIAANLQLIHAMGLTEADVYLNMLPLFHIAG-LNLALATFHAGGANVVMEKFDPAEALEL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 310 faapflptngtaTTAPPISFFTVVPTVYSRLLAshktlspqmqAATSEATSPANLRvNISGsaaLPTP-VKAAWAALSGG 388
Cdd:cd17637 84 ------------IEEEKVTLMGSFPPILSNLLD----------AAEKSGVDLSSLR-HVLG---LDAPeTIQRFEETTGA 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 389 NVLLeRYGMTEVGMaLSCGLAFADRvDASVGWPLPSVEARLVDmDTGDVIEPGhelddhgreRVGEIQLRGPTVFREYWR 468
Cdd:cd17637 138 TFWS-LYGQTETSG-LVTLSPYRER-PGSAGRPGPLVRVRIVD-DNDRPVPAG---------ETGEIVVRGPLVFQGYWN 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 469 NPDATAKEFvddggdgRGRWFKTGDVairrepvgaaGRNShrqpwAQGPMYFiQGRKTA-DIIKTGGEKVSALEVERELL 547
Cdd:cd17637 205 LPELTAYTF-------RNGWHHTGDL----------GRFD-----EDGYLWY-AGRKPEkELIKPGGENVYPAEVEKVIL 261
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 573989775 548 SLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINK 621
Cdd:cd17637 262 EHPAIAEVCVIGVPDPKWGEGIKAVCVLKPGATL--TADELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
68-628 |
3.97e-38 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 148.74 E-value: 3.97e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 68 EAVAAHdPTSTAVVHALsGRRFTYGELlgDVARARERLREAVDG-KPltGERVAFLIENSYDYVVpeahsrdvvMFLAVM 146
Cdd:PRK06087 31 QTARAM-PDKIAVVDNH-GASYTYSAL--DHAASRLANWLLAKGiEP--GDRVAFQLPGWCEFTI---------IYLACL 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 147 AARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQ-----KPVYF---ELPKHTG--------SS 210
Cdd:PRK06087 96 KVGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNqlpqlQQIVGvdkLAPATSSlslsqiiaDY 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 211 HGAKEAVSLADEDFSGaglMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLF 290
Cdd:PRK06087 176 EPLTTAITTHGDELAA---VLFTSGTEGLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFL 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 291 AGSSVEFMFPFNADA----------VWKRFAAPFlptngtattappisfftvvptVYSRLlashKTLspqmQAATSEATS 360
Cdd:PRK06087 253 IGARSVLLDIFTPDAclalleqqrcTCMLGATPF---------------------IYDLL----NLL----EKQPADLSA 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 361 panLRVNISGSAalPTPVKAAWAALSGGNVLLERYGMTEvgmalSCGLAFA------DRVDASVGWPLPSVEARLVDMDT 434
Cdd:PRK06087 304 ---LRFFLCGGT--TIPKKVARECQQRGIKLLSVYGSTE-----SSPHAVVnlddplSRFMHTDGYAAAGVEIKVVDEAR 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 435 GDViEPGHElddhgrervGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaAGRNShrqpwa 514
Cdd:PRK06087 374 KTL-PPGCE---------GEEASRGPNVFMGYLDEPELTARALDEEG------WYYSGDLCRMDE----AGYIK------ 427
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 515 qgpmyfIQGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAvkwTP-LEMRRAL- 592
Cdd:PRK06087 428 ------ITGRKK-DIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHH---SLtLEEVVAFf 497
|
570 580 590
....*....|....*....|....*....|....*..
gi 573989775 593 -KSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLVKAI 628
Cdd:PRK06087 498 sRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRKDI 534
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
71-624 |
5.74e-38 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 147.34 E-value: 5.74e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHalSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARA 150
Cdd:PRK06145 12 ARRTPDRAALVY--RDQEISYAEFHQRILQAAGMLHARGIGQ---GDVVALLMKNSAAFLE---------LAFAASYLGA 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 151 IAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYFELPKHTGSSHGAKEAVSLADEDFSgagLM 230
Cdd:PRK06145 78 VFLPINYRLAADEVAYILGDAGAKLLLVDEEFDAIVALETPKIVIDAAAQADSRRLAQGGLEIPPQAAVAPTDLV---RL 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 231 LYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAVWKRF 310
Cdd:PRK06145 155 MYTSGTTDRPKGVMHSYGNLHWKSIDHVIALGLTASERLLVVGPLYHVGAFDLPGIAVLWVGGTLRIHREFDPEAVLAAI 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 311 AAPFLPTNGTAttappisfftvvPTVYSRLLAshktlspqmqAATSEATSPANLRVNISGSAALPTPVKAAWAALSGGNV 390
Cdd:PRK06145 235 ERHRLTCAWMA------------PVMLSRVLT----------VPDRDRFDLDSLAWCIGGGEKTPESRIRDFTRVFTRAR 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 391 LLERYGMTEvgmalSCG---LAFADR-VD--ASVGWPLPSVEARLVDmDTGDVIEPGHElddhgrervGEIQLRGPTVFR 464
Cdd:PRK06145 293 YIDAYGLTE-----TCSgdtLMEAGReIEkiGSTGRALAHVEIRIAD-GAGRWLPPNMK---------GEICMRGPKVTK 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 465 EYWRNPDATAKEFVDDggdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIQGRKtaDIIKTGGEKVSALEVER 544
Cdd:PRK06145 358 GYWKDPEKTAEAFYGD-------WFRSGDVGYLDE---------------EGFLYLTDRKK--DMIISGGENIASSEVER 413
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 545 ELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK06145 414 VIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGATL--TLEALDRHCRQRLASFKVPRQLKVRDELPRNPSGKVLKRVL 491
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
230-624 |
2.58e-37 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 142.42 E-value: 2.58e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 230 MLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFP-FNADAVW- 307
Cdd:cd05917 7 IQFTSGTTGSPKGATLTHHNIVNNGYFIGERLGLTEQDRLCIPVPLFHCFGSVLGVLACLTHGATMVFPSPsFDPLAVLe 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 308 --KRFAAPFLptNGtattappisfftvVPTVYSRLLASHKTLSpqmqaatseaTSPANLRVNISGSAALPTPVKAAwaal 385
Cdd:cd05917 87 aiEKEKCTAL--HG-------------VPTMFIAELEHPDFDK----------FDLSSLRTGIMAGAPCPPELMKR---- 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 386 sggnvLLERYGMTEV----GMALSCGLAFADRVDAS-------VGWPLPSVEARLVDMDTGDVIEPGhelddhgreRVGE 454
Cdd:cd05917 138 -----VIEVMNMKDVtiayGMTETSPVSTQTRTDDSiekrvntVGRIMPHTEAKIVDPEGGIVPPVG---------VPGE 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 455 IQLRGPTVFREYWRNPDATAKEFVDDggdgrgRWFKTGDVAIRREpvgaagrnshrqpwaQGpMYFIQGRkTADIIKTGG 534
Cdd:cd05917 204 LCIRGYSVMKGYWNDPEKTAEAIDGD------GWLHTGDLAVMDE---------------DG-YCRIVGR-IKDMIIRGG 260
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 535 EKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHavKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRN 614
Cdd:cd05917 261 ENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKEGA--ELTEEDIKAYCKGKIAHYKVPRYVFFVDEFPLT 338
|
410
....*....|
gi 573989775 615 AMGKINKKQL 624
Cdd:cd05917 339 VSGKIQKFKL 348
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
66-618 |
2.86e-37 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 145.80 E-value: 2.86e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVHAlsGRRFTYGELlgdVARAReRLREAVDGKPL-TGERVAFLIENSYDYVVpeahsrdvvMFLA 144
Cdd:PRK07798 8 LFEAVADAVPDRVALVCG--DRRLTYAEL---EERAN-RLAHYLIAQGLgPGDHVGIYARNRIEYVE---------AMLG 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 145 VMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLAtdlqqkpvyfELPK-HT-------GSSHGAKEA 216
Cdd:PRK07798 73 AFKARAVPVNVNYRYVEDELRYLLDDSDAVALVYEREFAPRVAEVLP----------RLPKlRTlvvvedgSGNDLLPGA 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 217 VSLADEDFSGAG------------LMLYTSGTTNRPKGVL----------------LPEPVITAQCRSLLQAWEySPADR 268
Cdd:PRK07798 143 VDYEDALAAGSPerdfgerspddlYLLYTGGTTGMPKGVMwrqedifrvllggrdfATGEPIEDEEELAKRAAA-GPGMR 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 269 LLHVLPLhhIHGT-INAVLTPLFAGSSVEF--MFPFNADAVWKRFAApflptNGtattappISFFTVVPTVYSRLLAshK 345
Cdd:PRK07798 222 RFPAPPL--MHGAgQWAAFAALFSGQTVVLlpDVRFDADEVWRTIER-----EK-------VNVITIVGDAMARPLL--D 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 346 TLSPQMQAATSeatspaNLRVNISGSAALPTPVKAAWAALSGGNVLLERYGMTEVGmalSCGLAFADRVDASVGWPL--P 423
Cdd:PRK07798 286 ALEARGPYDLS------SLFAIASGGALFSPSVKEALLELLPNVVLTDSIGSSETG---FGGSGTVAKGAVHTGGPRftI 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 424 SVEARLVDMDTGDViEPGHElddhgreRVGEIQLRGPTVFReYWRNPDATAKEFVDDGGDgrgRWFKTGDVAiRREPVGA 503
Cdd:PRK07798 357 GPRTVVLDEDGNPV-EPGSG-------EIGWIARRGHIPLG-YYKDPEKTAETFPTIDGV---RYAIPGDRA-RVEADGT 423
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 504 A---GRNSHrqpwaqgpmyfiqgrktadIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHA 580
Cdd:PRK07798 424 ItllGRGSV-------------------CINTGGEKVFPEEVEEALKAHPDVADALVVGVPDERWGQEVVAVVQLREGAR 484
|
570 580 590
....*....|....*....|....*....|....*...
gi 573989775 581 VkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGK 618
Cdd:PRK07798 485 P--DLAELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
66-628 |
9.43e-37 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 144.52 E-value: 9.43e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAvdG-KPltGERVAFLIENSYDYVVpeahsrdvvMFLA 144
Cdd:COG1021 30 LLRRRAERHPDRIAVVDG--ERRLSYAELDRRADRLAAGLLAL--GlRP--GDRVVVQLPNVAEFVI---------VFFA 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 145 VMAARAIAVPLSPPFPAPELQYILDQSAASVLVAS---PKFAAKArevLATDLQQKpvyFELPKHTGSSHGAKEAVSLAD 221
Cdd:COG1021 95 LFRAGAIPVFALPAHRRAEISHFAEQSEAVAYIIPdrhRGFDYRA---LARELQAE---VPSLRHVLVVGDAGEFTSLDA 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 222 -----EDFSGAG-------LMLYTSGTTNRPKgvLLPEP----VITAQCRSllQAWEYSPADRLLHVLPLHHihgtiNA- 284
Cdd:COG1021 169 llaapADLSEPRpdpddvaFFQLSGGTTGLPK--LIPRThddyLYSVRASA--EICGLDADTVYLAALPAAH-----NFp 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 285 -----VLTPLFAGSSVefmfpfnadaVwkrfaapfLPTNGTATTAPP------ISFFTVVPTVYSRLLashktlspqmQA 353
Cdd:COG1021 240 lsspgVLGVLYAGGTV----------V--------LAPDPSPDTAFPliererVTVTALVPPLALLWL----------DA 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 354 ATSEATSPANLRVNISGSAAL-PTPVKAAWAALsgGNVLLERYGMTEvgmalscGLAFADRVDAS-------VGWPL-PS 424
Cdd:COG1021 292 AERSRYDLSSLRVLQVGGAKLsPELARRVRPAL--GCTLQQVFGMAE-------GLVNYTRLDDPeevilttQGRPIsPD 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 425 VEARLVDmDTGDVIEPGHelddhgrerVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAiRREPVGAa 504
Cdd:COG1021 363 DEVRIVD-EDGNPVPPGE---------VGELLTRGPYTIRGYYRAPEHNARAFTPDG------FYRTGDLV-RRTPDGY- 424
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 505 grnshrqpwaqgpmYFIQGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDpDHAVkwT 584
Cdd:COG1021 425 --------------LVVEGRAK-DQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPR-GEPL--T 486
|
570 580 590 600
....*....|....*....|....*....|....*....|....*
gi 573989775 585 PLEMRRALKSR-LAGYKIPQVMKIVNHIPRNAMGKINKKQLVKAI 628
Cdd:COG1021 487 LAELRRFLRERgLAAFKLPDRLEFVDALPLTAVGKIDKKALRAAL 531
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
67-636 |
8.43e-36 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 142.22 E-value: 8.43e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 67 FEAVAAHDPTSTAVVHALSGRRFTYGELLGDVAR-ARERLREAVDgkplTGERVAFLIENSYDYVVPEahsrdvvmfLAV 145
Cdd:PRK12583 24 FDATVARFPDREALVVRHQALRYTWRQLADAVDRlARGLLALGVQ----PGDRVGIWAPNCAEWLLTQ---------FAT 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFaaKAREVLATDLQQKP---------------------VYF--E 202
Cdd:PRK12583 91 ARIGAILVNINPAYRASELEYALGQSGVRWVICADAF--KTSDYHAMLQELLPglaegqpgalacerlpelrgvVSLapA 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 203 LPKHTGSSH---GAKEAVSLADEDFSGAGL-------MLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHV 272
Cdd:PRK12583 169 PPPGFLAWHelqARGETVSREALAERQASLdrddpinIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDRLCVP 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 273 LPLHHIHGTINAVLTPLFAGSSVefMFP---FNADAVWKRFAApflptngTATTAppisfFTVVPTVYsrllashktlsp 349
Cdd:PRK12583 249 VPLYHCFGMVLANLGCMTVGACL--VYPneaFDPLATLQAVEE-------ERCTA-----LYGVPTMF------------ 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 350 qmqaaTSEATSP-------ANLRVNISGSAALPTPV-KAAWAALSGGNVLLErYGMTEVGmALSCGLAFADRVD---ASV 418
Cdd:PRK12583 303 -----IAELDHPqrgnfdlSSLRTGIMAGAPCPIEVmRRVMDEMHMAEVQIA-YGMTETS-PVSLQTTAADDLErrvETV 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 419 GWPLPSVEARLVDMDtGDVIEPGHelddhgrerVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRR 498
Cdd:PRK12583 376 GRTQPHLEVKVVDPD-GATVPRGE---------IGELCTRGYSVMKGYWNNPEATAESIDEDG------WMHTGDLATMD 439
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 499 EpvgaagrnshrqpwaQGpmYF-IQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDP 577
Cdd:PRK12583 440 E---------------QG--YVrIVGR-SKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRLHP 501
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*....
gi 573989775 578 DHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLvKAIFVEETSGD 636
Cdd:PRK12583 502 GHAA--SEEELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQKFRM-REISIEELALP 557
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
67-624 |
2.40e-35 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 140.57 E-value: 2.40e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 67 FEAVAAHDPTSTAVVHALSG----RRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMF 142
Cdd:PRK13295 30 LDACVASCPDKTAVTAVRLGtgapRRFTYRELAALVDRVAVGLARLGVGR---GDVVSCQLPNWWEFTV---------LY 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 143 LAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVAsPK------FAAKAREV--LATDLQQ-------KPVYFELPKHT 207
Cdd:PRK13295 98 LACSRIGAVLNPLMPIFRERELSFMLKHAESKVLVV-PKtfrgfdHAAMARRLrpELPALRHvvvvggdGADSFEALLIT 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 208 GSSHGAKEAVSLADEDFSGAG---LMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINA 284
Cdd:PRK13295 177 PAWEQEPDAPAILARLRPGPDdvtQLIYTSGTTGEPKGVMHTANTLMANIVPYAERLGLGADDVILMASPMAHQTGFMYG 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 285 VLTPLFAGSSVEFMfpfnadAVW-KRFAAPFLPTNGTAttappisfFTvvptvysrlLASHKTLSPQMQAATSEATSPAN 363
Cdd:PRK13295 257 LMMPVMLGATAVLQ------DIWdPARAAELIRTEGVT--------FT---------MASTPFLTDLTRAVKESGRPVSS 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 364 LRVNISGSAALPTP-VKAAWAALsgGNVLLERYGMTEVGMALSCGLAFAD-RVDASVGWPLPSVEARLVDMDtGDVIEPG 441
Cdd:PRK13295 314 LRTFLCAGAPIPGAlVERARAAL--GAKIVSAWGMTENGAVTLTKLDDPDeRASTTDGCPLPGVEVRVVDAD-GAPLPAG 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 442 HElddhgrervGEIQLRGPTVFREYWRNPDATAkefvdDGGDGrgrWFKTGDVAiRREPVGAAGrnshrqpwaqgpmyfI 521
Cdd:PRK13295 391 QI---------GRLQVRGCSNFGGYLKRPQLNG-----TDADG---WFDTGDLA-RIDADGYIR---------------I 437
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 522 QGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKS-RLAGYK 600
Cdd:PRK13295 438 SGR-SKDVIIRGGENIPVVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVPRPGQSL--DFEEMVEFLKAqKVAKQY 514
|
570 580
....*....|....*....|....
gi 573989775 601 IPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK13295 515 IPERLVVRDALPRTPSGKIQKFRL 538
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
75-624 |
2.86e-35 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 138.66 E-value: 2.86e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHAlsGRRFTYGELLGDVARARERLREAvdgKPLTGERVAFLIENSydyvvPEAhsrdVVMFLAVMAARAIAVP 154
Cdd:cd17649 1 PDAVALVFG--DQSLSYAELDARANRLAHRLRAL---GVGPEVRVGIALERS-----LEM----VVALLAILKAGGAYVP 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 155 LSPPFPAPELQYILDQSAASVLVaspkfaakarevlatdlqqkpvyfelpkhtgSSHGAKEAvsladedfsgagLMLYTS 234
Cdd:cd17649 67 LDPEYPAERLRYMLEDSGAGLLL-------------------------------THHPRQLA------------YVIYTS 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 235 GTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHhIHGTINAVLTPLFAGSSVEFmfpfnadavwkRFAAPF 314
Cdd:cd17649 104 GSTGTPKGVAVSHGPLAAHCQATAERYGLTPGDRELQFASFN-FDGAHEQLLPPLICGACVVL-----------RPDELW 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 315 LPTNGTATTappISFFTV----VPTVYSRLLASHktlspqmqAATSEATSPANLRVNISGSAALPtpVKAAWAALSGGNV 390
Cdd:cd17649 172 ASADELAEM---VRELGVtvldLPPAYLQQLAEE--------ADRTGDGRPPSLRLYIFGGEALS--PELLRRWLKAPVR 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 391 LLERYGMTE-VGMALSC-GLAFADRVDASV--GWPLPSVEARLVDMDTGDViEPGhelddhgreRVGEIQLRGPTVFREY 466
Cdd:cd17649 239 LFNAYGPTEaTVTPLVWkCEAGAARAGASMpiGRPLGGRSAYILDADLNPV-PVG---------VTGELYIGGEGLARGY 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 467 WRNPDATAKEFVDD--GGDGrGRWFKTGDVAIRREP--VGAAGRNSHRqpwaqgpmyfiqgrktadiIKTGGEKVSALEV 542
Cdd:cd17649 309 LGRPELTAERFVPDpfGAPG-SRLYRTGDLARWRDDgvIEYLGRVDHQ-------------------VKIRGFRIELGEI 368
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 543 ERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVKWtPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKK 622
Cdd:cd17649 369 EAALLEHPGVREAAVVALDGAGGKQLVAYVVLRAAAAQPEL-RAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRK 447
|
..
gi 573989775 623 QL 624
Cdd:cd17649 448 AL 449
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
87-624 |
6.77e-35 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 138.66 E-value: 6.77e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 87 RRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSydyvvPEahsrdvVMF----LAVMAAraIAVPLSPPFPAP 162
Cdd:PRK08008 36 RRYSYLELNEEINRTANLFYSLGIRK---GDKVALHLDNC-----PE------FIFcwfgLAKIGA--IMVPINARLLRE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 163 ELQYILDQSAASVLVASPKFAAKAREVLATD---LQQKPVYFE-LPKHTGSSH----------GAKEAVSLADEDFSGag 228
Cdd:PRK08008 100 ESAWILQNSQASLLVTSAQFYPMYRQIQQEDatpLRHICLTRVaLPADDGVSSftqlkaqqpaTLCYAPPLSTDDTAE-- 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 lMLYTSGTTNRPKGVllpepVITaQCrSLLQAWEYSP-------ADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPF 301
Cdd:PRK08008 178 -ILFTSGTTSRPKGV-----VIT-HY-NLRFAGYYSAwqcalrdDDVYLTVMPAFHIDCQCTAAMAAFSAGATFVLLEKY 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 302 NADAVWK---RFAApflptngTATTAPPISFftvvptvysrllashKTLSPQMQAATSEATSpanLR-----VNISGSAA 373
Cdd:PRK08008 250 SARAFWGqvcKYRA-------TITECIPMMI---------------RTLMVQPPSANDRQHC---LRevmfyLNLSDQEK 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 374 LptpvkaawAALSGGNV-LLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDmDTGDVIEPGhelddhgreRV 452
Cdd:PRK08008 305 D--------AFEERFGVrLLTSYGMTETIVGIIGDRPGDKRRWPSIGRPGFCYEAEIRD-DHNRPLPAG---------EI 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 453 GEIQLRG---PTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIQgRKTaDI 529
Cdd:PRK08008 367 GEICIKGvpgKTIFKEYYLDPKATAKVLEADG------WLHTGDTGYVDE---------------EGFFYFVD-RRC-NM 423
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 530 IKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPdhAVKWTPLEMRRALKSRLAGYKIPQVMKIVN 609
Cdd:PRK08008 424 IKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNE--GETLSEEEFFAFCEQNMAKFKVPSYLEIRK 501
|
570
....*....|....*
gi 573989775 610 HIPRNAMGKINKKQL 624
Cdd:PRK08008 502 DLPRNCSGKIIKKNL 516
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
67-624 |
6.93e-35 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 138.25 E-value: 6.93e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 67 FEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGkplTGERVAFLIENSYDYVVPeahsrdvvmFLAVM 146
Cdd:cd17651 1 FERQAARTPDAPALVAE--GRRLTYAELDRRANRLAHRLRARGVG---PGDLVALCARRSAELVVA---------LLAIL 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 147 AARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPvyFELPKHTGSSHG-AKEAVSLAdedfs 225
Cdd:cd17651 67 KAGAAYVPLDPAYPAERLAFMLADAGPVLVLTHPALAGELAVELVAVTLLDQ--PGAAAGADAEPDpALDADDLA----- 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 226 gagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRllhvlplhhihgtinavlTPLFAGSSvefmfpFNAdA 305
Cdd:cd17651 140 ---YVIYTSGSTGRPKGVVMPHRSLANLVAWQARASSLGPGAR------------------TLQFAGLG------FDV-S 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 306 VWKRFAApfLPTNGTATTAPP-------------------ISFFtvvPTVYSRLLASHktlspqmqaATSEATSPANLR- 365
Cdd:cd17651 192 VQEIFST--LCAGATLVLPPEevrtdppalaawldeqrisRVFL---PTVALRALAEH---------GRPLGVRLAALRy 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 366 VNISGSAALPTPVKAAWAALSGGNVLLERYGMTE--VGMALSCGLAFADRVD-ASVGWPLPSVEARLvdmdtgdviepgh 442
Cdd:cd17651 258 LLTGGEQLVLTEDLREFCAGLPGLRLHNHYGPTEthVVTALSLPGDPAAWPApPPIGRPIDNTRVYV------------- 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 443 eLDDHGRE----RVGEIQLRGPTVFREYWRNPDATAKEFVDDGGDGRGRWFKTGDVAiRREPVGA---AGRNShrqpwaq 515
Cdd:cd17651 325 -LDAALRPvppgVPGELYIGGAGLARGYLNRPELTAERFVPDPFVPGARMYRTGDLA-RWLPDGElefLGRAD------- 395
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 516 gpmyfiqgrktaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSR 595
Cdd:cd17651 396 ------------DQVKIRGFRIELGEIEAALARHPGVREAVVLAREDRPGEKRLVAYVVGDPEAPV--DAAELRAALATH 461
|
570 580
....*....|....*....|....*....
gi 573989775 596 LAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd17651 462 LPEYMVPSAFVLLDALPLTPNGKLDRRAL 490
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
61-622 |
1.13e-34 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 138.48 E-value: 1.13e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 61 LPRLP-IFEAVAAHDPTSTAVVHALSGRRFTYGELLGDVARARERLREAvdgKPLTGERVAFLIENSYDYVVPeahsrdv 139
Cdd:PRK05852 15 GPRIAdLVEVAATRLPEAPALVVTADRIAISYRDLARLVDDLAGQLTRS---GLLPGDRVALRMGSNAEFVVA------- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 140 vmFLAVMAARAIAVPLSPPFPAPELqyiLDQSAAsvlvaspkfaAKAREVLATDL---QQKPV---YFELPKHTGSSHGA 213
Cdd:PRK05852 85 --LLAASRADLVVVPLDPALPIAEQ---RVRSQA----------AGARVVLIDADgphDRAEPttrWWPLTVNVGGDSGP 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 214 KEAVSLADEDFSGA---------------GLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHI 278
Cdd:PRK05852 150 SGGTLSVHLDAATEptpatstpeglrpddAMIMFTGGTTGLPKMVPWTHANIASSVRAIITGYRLSPRDATVAVMPLYHG 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 279 HGTINAVLTPLFAGSSVefMFP----FNADAVWKRFAApflptnGTATtappisFFTVVPTVYSRLLashktlspQMQAA 354
Cdd:PRK05852 230 HGLIAALLATLASGGAV--LLPargrFSAHTFWDDIKA------VGAT------WYTAVPTIHQILL--------ERAAT 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 355 TSEATSPANLRVNISGSAALpTPVKAAWAALSGGNVLLERYGMTEVGMALScglafADRVDASVGWPLPSVEARLVDMDT 434
Cdd:PRK05852 288 EPSGRKPAALRFIRSCSAPL-TAETAQALQTEFAAPVVCAFGMTEATHQVT-----TTQIEGIGQTENPVVSTGLVGRST 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 435 GDVIE----PGHELddhGRERVGEIQLRGPTVFREYWRNPDATAKEFVDDggdgrgrWFKTGDVAirrePVGAAGRNShr 510
Cdd:PRK05852 362 GAQIRivgsDGLPL---PAGAVGEVWLRGTTVVRGYLGDPTITAANFTDG-------WLRTGDLG----SLSAAGDLS-- 425
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 511 qpwaqgpmyfIQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIIldPDHAVKWTPLEMRR 590
Cdd:PRK05852 426 ----------IRGR-IKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVAAVIV--PRESAPPTAEELVQ 492
|
570 580 590
....*....|....*....|....*....|..
gi 573989775 591 ALKSRLAGYKIPQVMKIVNHIPRNAMGKINKK 622
Cdd:PRK05852 493 FCRERLAAFEIPASFQEASGLPHTAKGSLDRR 524
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
71-626 |
1.63e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 138.25 E-value: 1.63e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVhaLSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENsydyvVPEAHsrdvVMFLAVMAARA 150
Cdd:PRK06178 43 ARERPQRPAII--FYGHVITYAELDELSDRFAALLRQRGVGA---GDRVAVFLPN-----CPQFH----IVFFGILKLGA 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 151 IAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKA---------REVLATDLQQ-------KPVYFEL----PKHTG-- 208
Cdd:PRK06178 109 VHVPVSPLFREHELSYELNDAGAEVLLALDQLAPVVeqvraetslRHVIVTSLADvlpaeptLPLPDSLraprLAAAGai 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 209 ----SSHGAKEAVSLADEDFSGAGLMLYTSGTTNRPKGVLLPEP--VITAQCRSLLQAWEYSPaDRLLHVLPLHHIHGTI 282
Cdd:PRK06178 189 dllpALRACTAPVPLPPPALDALAALNYTGGTTGMPKGCEHTQRdmVYTAAAAYAVAVVGGED-SVFLSFLPEFWIAGEN 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 283 NAVLTPLFAGSSVEFMFPFNADAVwkrFAApfLPTNGTATTAPPISffTVVPtvysrlLASHKtlspqmQAATSEATSPA 362
Cdd:PRK06178 268 FGLLFPLFSGATLVLLARWDAVAF---MAA--VERYRVTRTVMLVD--NAVE------LMDHP------RFAEYDLSSLR 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 363 NLRVnISGSAALPTPVKAAWAALSGGNVLLERYGMTEVGMALSCGLAF-ADRVDAS-----VGWPLPSVEARLVDMDTGD 436
Cdd:PRK06178 329 QVRV-VSFVKKLNPDYRQRWRALTGSVLAEAAWGMTETHTCDTFTAGFqDDDFDLLsqpvfVGLPVPGTEFKICDFETGE 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 437 VIEPGHElddhgrervGEIQLRGPTVFREYWRNPDATAKEFVDDggdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQG 516
Cdd:PRK06178 408 LLPLGAE---------GEIVVRTPSLLKGYWNKPEATAEALRDG-------WLHTGDIGKIDE---------------QG 456
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 517 PMYFIQGRKtaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRL 596
Cdd:PRK06178 457 FLHYLGRRK--EMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGADL--TAAALQAWCRENM 532
|
570 580 590
....*....|....*....|....*....|
gi 573989775 597 AGYKIPQVmKIVNHIPRNAMGKINKKQLVK 626
Cdd:PRK06178 533 AVYKVPEI-RIVDALPMTATGKVRKQDLQA 561
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
110-625 |
2.42e-34 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 136.03 E-value: 2.42e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 110 DGKPLTGERVAFLIENSYDYV-----VPEAHSRDVVMFlavmaaraiaVPLSPPFPAPELQYILDQSAASVLVASPKFAA 184
Cdd:cd05922 12 EAGGVRGERVVLILPNRFTYIelsfaVAYAGGRLGLVF----------VPLNPTLKESVLRYLVADAGGRIVLADAGAAD 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 185 KAREVLaTDLQQKPVYFELPKHTGSSHGAkEAVSLADEDFSgagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYS 264
Cdd:cd05922 82 RLRDAL-PASPDPGTVLDADGIRAARASA-PAHEVSHEDLA---LLLYTSGSTGSPKLVRLSHQNLLANARSIAEYLGIT 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 265 PADRLLHVLPLHHIHGTiNAVLTPLFAGSSV----EFMFPfnaDAVWKRFAApflpTNGTAttappisfFTVVPTVYSRL 340
Cdd:cd05922 157 ADDRALTVLPLSYDYGL-SVLNTHLLRGATLvltnDGVLD---DAFWEDLRE----HGATG--------LAGVPSTYAML 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 341 LashktlspQMQAATSEATSpanLRVNISGSAALPTPVKAAWAALSGGNVLLERYGMTEVgmalSCGLAF--ADRVD--- 415
Cdd:cd05922 221 T--------RLGFDPAKLPS---LRYLTQAGGRLPQETIARLRELLPGAQVYVMYGQTEA----TRRMTYlpPERILekp 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 416 ASVGWPLPSVEArLVDMDTGDVIEPGhelddhgreRVGEIQLRGPTVFREYWRNPDATAKEfvdDGGDGRGRwfkTGDVA 495
Cdd:cd05922 286 GSIGLAIPGGEF-EILDDDGTPTPPG---------EPGEIVHRGPNVMKGYWNDPPYRRKE---GRGGGVLH---TGDLA 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 496 IRREpvgaagrnshrqpwaQGpMYFIQGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSgNWGQKVGAVIIL 575
Cdd:cd05922 350 RRDE---------------DG-FLFIVGRRD-RMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPD-PLGEKLALFVTA 411
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 573989775 576 DPdhavKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLV 625
Cdd:cd05922 412 PD----KIDPKDVLRSLAERLPPYKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
71-624 |
1.06e-33 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 134.30 E-value: 1.06e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAvdgKPLTGERVAFLIENSYDYVVPeahsrdvvmFLAVMAARA 150
Cdd:cd05945 1 AAANPDRPAVVEG--GRTLTYRELKERADALAAALASL---GLDAGDPVVVYGHKSPDAIAA---------FLAALKAGH 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 151 IAVPLSPPFPAPELQYILDQSAASVLVASPKfaakarevlatdlqqKPVYfelpkhtgsshgakeavsladedfsgaglM 230
Cdd:cd05945 67 AYVPLDASSPAERIREILDAAKPALLIADGD---------------DNAY-----------------------------I 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 231 LYTSGTTNRPKGVllpepVITAQCRSLLQAWEYS-----PADRLLHVLPLH---HIHGtinaVLTPLFAGSSVefmFPFN 302
Cdd:cd05945 103 IFTSGSTGRPKGV-----QISHDNLVSFTNWMLSdfplgPGDVFLNQAPFSfdlSVMD----LYPALASGATL---VPVP 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 303 ADAVwKRFAAPF--LPTNGtattappISFFTVVPTVYsRLLASHKTLSPQMQAatseatspaNLRVNISGSAALPTPVKA 380
Cdd:cd05945 171 RDAT-ADPKQLFrfLAEHG-------ITVWVSTPSFA-AMCLLSPTFTPESLP---------SLRHFLFCGEVLPHKTAR 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 381 AWAALSGGNVLLERYGMTEVGMALScglafADRVDASV---------GWPLPSVEARLVDMDtGDVIEPGhelddhgreR 451
Cdd:cd05945 233 ALQQRFPDARIYNTYGPTEATVAVT-----YIEVTPEVldgydrlpiGYAKPGAKLVILDED-GRPVPPG---------E 297
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 452 VGEIQLRGPTVFREYWRNPDATAKEFVDDGGdgrGRWFKTGDVAiRREPVGaagrnshrqpwaqgpMYFIQGRKTaDIIK 531
Cdd:cd05945 298 KGELVISGPSVSKGYLNNPEKTAAAFFPDEG---QRAYRTGDLV-RLEADG---------------LLFYRGRLD-FQVK 357
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 532 TGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPdHAVKWTPLEMRRALKSRLAGYKIPQVMKIVNHI 611
Cdd:cd05945 358 LNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIAFVVPKP-GAEAGLTKAIKAELAERLPPYMIPRRFVYLDEL 436
|
570
....*....|...
gi 573989775 612 PRNAMGKINKKQL 624
Cdd:cd05945 437 PLNANGKIDRKAL 449
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
88-547 |
1.54e-33 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 134.02 E-value: 1.54e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 88 RFTYGELLGDVARARERLReAVDGKPltGERVAFLIENSYDYVVPEahsrdvvmfLAVMAARAIAVPLSPPFPAPELQYI 167
Cdd:cd17640 5 RITYKDLYQEILDFAAGLR-SLGVKA--GEKVALFADNSPRWLIAD---------QGIMALGAVDVVRGSDSSVEELLYI 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 168 LDQSAASVLvaspkFAAKAREVLATdlqqkpvyfelpkhtgsshgakeavsladedfsgaglMLYTSGTTNRPKGVLLPE 247
Cdd:cd17640 73 LNHSESVAL-----VVENDSDDLAT-------------------------------------IIYTSGTTGNPKGVMLTH 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 248 PVITAQCRSLLQAWEYSPADRLLHVLPLHHIhgtinavltplfAGSSVEFmFPFNADAVWKRFAAPFLPtNGTATTAPpi 327
Cdd:cd17640 111 ANLLHQIRSLSDIVPPQPGDRFLSILPIWHS------------YERSAEY-FIFACGCSQAYTSIRTLK-DDLKRVKP-- 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 328 SFFTVVP----TVYSRLLASHKTLSPQMQAATSEATSPANLRVNISGSAALPTPVKAAWAALsgGNVLLERYGMTEVGMA 403
Cdd:cd17640 175 HYIVSVPrlweSLYSGIQKQVSKSSPIKQFLFLFFLSGGIFKFGISGGGALPPHVDTFFEAI--GIEVLNGYGLTETSPV 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 404 LSCGLaFADRVDASVGWPLPSVEARLVDMDTGDVIEPGHElddhgrervGEIQLRGPTVFREYWRNPDATAKEFVDDGgd 483
Cdd:cd17640 253 VSARR-LKCNVRGSVGRPLPGTEIKIVDPEGNVVLPPGEK---------GIVWVRGPQVMKGYYKNPEATSKVLDSDG-- 320
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 573989775 484 grgrWFKTGDVAIrrepvgaagrnshrqpWAQGPMYFIQGRKTADIIKTGGEKVSALEVERELL 547
Cdd:cd17640 321 ----WFNTGDLGW----------------LTCGGELVLTGRAKDTIVLSNGENVEPQPIEEALM 364
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
71-624 |
2.85e-33 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 133.61 E-value: 2.85e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARA 150
Cdd:cd05920 25 AARHPDRIAVVDG--DRRLTYRELDRRADRLAAGLRGLGIRP---GDRVVVQLPNVAEFVV---------LFFALLRLGA 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 151 IAVPLSPPFPAPELQYILDQSAASVLVASPKFA-----AKAREVLATdlQQKPVYFELpkhtgsshgakeavsladedfS 225
Cdd:cd05920 91 VPVLALPSHRRSELSAFCAHAEAVAYIVPDRHAgfdhrALARELAES--IPEVALFLL---------------------S 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 226 GaglmlytsGTTNRPKgvLLPEP------VITAQ---CRsllqaweYSPADRLLHVLPLHHihgtinavltplfagssve 296
Cdd:cd05920 148 G--------GTTGTPK--LIPRThndyayNVRASaevCG-------LDQDTVYLAVLPAAH------------------- 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 297 fMFPFNADAVWKRFAApflptNGTATTAPPISFFTVVPTVySRLLASHKTLSPQ-----MQAATSEATSPANLRVNISGS 371
Cdd:cd05920 192 -NFPLACPGVLGTLLA-----GGRVVLAPDPSPDAAFPLI-EREGVTVTALVPAlvslwLDAAASRRADLSSLRLLQVGG 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 372 AAL-PTPVKAAWAALsgGNVLLERYGMTEvgmALSCGLAF---ADRVDASVGWPL-PSVEARLVDMDTGDViEPGHEldd 446
Cdd:cd05920 265 ARLsPALARRVPPVL--GCTLQQVFGMAE---GLLNYTRLddpDEVIIHTQGRPMsPDDEIRVVDEEGNPV-PPGEE--- 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 447 hgrervGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVaIRREPVGaagrNSHrqpwaqgpmyfIQGRkT 526
Cdd:cd05920 336 ------GELLTRGPYTIRGYYRAPEHNARAFTPDG------FYRTGDL-VRRTPDG----YLV-----------VEGR-I 386
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 527 ADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPdhaVKWTPLEMRRALKSR-LAGYKIPQVM 605
Cdd:cd05920 387 KDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLGERSCAFVVLRD---PPPSAAQLRRFLRERgLAAYKLPDRI 463
|
570
....*....|....*....
gi 573989775 606 KIVNHIPRNAMGKINKKQL 624
Cdd:cd05920 464 EFVDSLPLTAVGKIDKKAL 482
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
86-633 |
2.94e-33 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 134.50 E-value: 2.94e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 86 GRRFTYGELLGDVARARERLREAvdG-KPltGERVAFLIENSYDYVvpeahsrDVVMFLAVMAAraIAVPLSPPFPAPEL 164
Cdd:PRK06155 44 GTRWTYAEAARAAAAAAHALAAA--GvKR--GDRVALMCGNRIEFL-------DVFLGCAWLGA--IAVPINTALRGPQL 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 165 QYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYF---------ELPKHTGSSHGAKEAVSLADEDFSGAGLMLYTSG 235
Cdd:PRK06155 111 EHILRNSGARLLVVEAALLAALEAADPGDLPLPAVWLldapasvsvPAGWSTAPLPPLDAPAPAAAVQPGDTAAILYTSG 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 236 TTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGtINAVLTPLFAGSSVEFMFPFNADAVWKRFAApfl 315
Cdd:PRK06155 191 TTGPSKGVCCPHAQFYWWGRNSAEDLEIGADDVLYTTLPLFHTNA-LNAFFQALLAGATYVLEPRFSASGFWPAVRR--- 266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 316 pTNGTATTappisfftVVPTVYSRLLAShktlspqmqaATSEATSPANLRVNISGsaALPTPVKAAWAALSGGNvLLERY 395
Cdd:PRK06155 267 -HGATVTY--------LLGAMVSILLSQ----------PARESDRAHRVRVALGP--GVPAALHAAFRERFGVD-LLDGY 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 396 GMTEVGMAlsCGLAFADRVDASVGWPLPSVEARLVDmdtgdviEPGHELDDhgrERVGEIQLRG--PTVFRE-YWRNPDA 472
Cdd:PRK06155 325 GSTETNFV--IAVTHGSQRPGSMGRLAPGFEARVVD-------EHDQELPD---GEPGELLLRAdePFAFATgYFGMPEK 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 473 TAKEFvddggdgRGRWFKTGDVAIRRepvgaagrnshrqpwAQGPMYFIQGRKtaDIIKTGGEKVSALEVERELLSLPQV 552
Cdd:PRK06155 393 TVEAW-------RNLWFHTGDRVVRD---------------ADGWFRFVDRIK--DAIRRRGENISSFEVEQVLLSHPAV 448
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 553 AEAAVVAVPSGNWGQKVGAVIILDPDHAVKwtPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLVKAIFVEE 632
Cdd:PRK06155 449 AAAAVFPVPSELGEDEVMAAVVLRDGTALE--PVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQGVTAD 526
|
.
gi 573989775 633 T 633
Cdd:PRK06155 527 T 527
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
79-624 |
5.72e-33 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 132.72 E-value: 5.72e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 79 AVVHALSGRRFTYGELLGDVARARERLREAvdGKPlTGERVAFLIENSYDyvvpeahsrdvvmFLAVMAARAIA----VP 154
Cdd:PRK08276 2 AVIMAPSGEVVTYGELEARSNRLAHGLRAL--GLR-EGDVVAILLENNPE-------------FFEVYWAARRSglyyTP 65
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 155 LSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLAtdlqqkpvyfELPKH-------TGSSHG---------AKEAVS 218
Cdd:PRK08276 66 INWHLTAAEIAYIVDDSGAKVLIVSAALADTAAELAA----------ELPAGvplllvvAGPVPGfrsyeealaAQPDTP 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 219 LADEdfSGAGLMLYTSGTTNRPKGV---LLPEPVITAQCRSL--LQAWEYSPAD-RLLHVLPLHHihgtiNAVL----TP 288
Cdd:PRK08276 136 IADE--TAGADMLYSSGTTGRPKGIkrpLPGLDPDEAPGMMLalLGFGMYGGPDsVYLSPAPLYH-----TAPLrfgmSA 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 289 LFAGSSVEFMFPFNADAVWKrfaapflptngtATTAPPISFFTVVPTVYSRLLAshktLSPQMQAATSEATspanLRVNI 368
Cdd:PRK08276 209 LALGGTVVVMEKFDAEEALA------------LIERYRVTHSQLVPTMFVRMLK----LPEEVRARYDVSS----LRVAI 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 369 SGSAALPTPVKAA----WaalsgGNVLLERYGMTEVGmalscGLAFADRVDA-----SVGWPlpsVEARLvdmdtgdvie 439
Cdd:PRK08276 269 HAAAPCPVEVKRAmidwW-----GPIIHEYYASSEGG-----GVTVITSEDWlahpgSVGKA---VLGEV---------- 325
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 440 pgHELDDHGRE----RVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIrrepVGAAGrnshrqpwaq 515
Cdd:PRK08276 326 --RILDEDGNElppgEIGTVYFEMDGYPFEYHNDPEKTAAARNPHG------WVTVGDVGY----LDEDG---------- 383
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 516 gpmY-FIQGRKtADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVI------ILDPDHAVkwtplEM 588
Cdd:PRK08276 384 ---YlYLTDRK-SDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVqpadgaDAGDALAA-----EL 454
|
570 580 590
....*....|....*....|....*....|....*.
gi 573989775 589 RRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK08276 455 IAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRL 490
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
67-624 |
5.92e-32 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 129.70 E-value: 5.92e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 67 FEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVM 146
Cdd:cd17646 4 VAEQAARTPDAPAVVDE--GRTLTYRELDERANRLAHLLRARGVGP---EDRVAVLLPRSADLVV---------ALLAVL 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 147 AARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYFELPkhtgsshGAKEAVSLADEDfsg 226
Cdd:cd17646 70 KAGAAYLPLDPGYPADRLAYMLADAGPAVVLTTADLAARLPAGGDVALLGDEALAAPP-------ATPPLVPPRPDN--- 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 227 AGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLhhihgtinavltplfaGSSVefmfpfnadAV 306
Cdd:cd17646 140 LAYVIYTSGSTGRPKGVMVTHAGIVNRLLWMQDEYPLGPGDRVLQKTPL----------------SFDV---------SV 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 307 WKRFAApfLPTNGTATTAPPISFftVVPTVYSRLLASHKT----LSPQM-QAATSEATSP--ANLRVNISGSAALPTPVK 379
Cdd:cd17646 195 WELFWP--LVAGARLVVARPGGH--RDPAYLAALIREHGVttchFVPSMlRVFLAEPAAGscASLRRVFCSGEALPPELA 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 380 AAWAALSGGnVLLERYGMTEVGMALSCGLAFADRVDASV--GWPLPSVEARLVDmDTGDVIEPGhelddhgreRVGEIQL 457
Cdd:cd17646 271 ARFLALPGA-ELHNLYGPTEAAIDVTHWPVRGPAETPSVpiGRPVPNTRLYVLD-DALRPVPVG---------VPGELYL 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 458 RGPTVFREYWRNPDATAKEFVDDGGDGRGRWFKTGDVAiRREPVGA---AGRNSHRqpwaqgpmyfiqgrktadiIKTGG 534
Cdd:cd17646 340 GGVQLARGYLGRPALTAERFVPDPFGPGSRMYRTGDLA-RWRPDGAlefLGRSDDQ-------------------VKIRG 399
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 535 EKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVKwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRN 614
Cdd:cd17646 400 FRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARLVGYVVPAAGAAGP-DTAALRAHLAERLPEYMVPAAFVVLDALPLT 478
|
570
....*....|
gi 573989775 615 AMGKINKKQL 624
Cdd:cd17646 479 ANGKLDRAAL 488
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
71-626 |
7.14e-32 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 131.23 E-value: 7.14e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHALSG------RRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENsydyvVPEAHsrdvVMFLA 144
Cdd:PRK07529 35 AARHPDAPALSFLLDAdpldrpETWTYAELLADVTRTANLLHSLGVGP---GDVVAFLLPN-----LPETH----FALWG 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 145 VMAArAIAVPLSPPFPAPELQYILDQSAASVLVA---SPKF-----AAKARE-------VLATDL-------QQKPVYFE 202
Cdd:PRK07529 103 GEAA-GIANPINPLLEPEQIAELLRAAGAKVLVTlgpFPGTdiwqkVAEVLAalpelrtVVEVDLarylpgpKRLAVPLI 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 203 LPKHTGSSHGAKEAVS-------LADEDFSGAGLMLY--TSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVL 273
Cdd:PRK07529 182 RRKAHARILDFDAELArqpgdrlFSGRPIGPDDVAAYfhTGGTTGMPKLAQHTHGNEVANAWLGALLLGLGPGDTVFCGL 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 274 PLHHIHGTINAVLTPLFAGSSVEFMFP--FNADAVWKRFAApflptngtATTAPPISFFTVVPTVYSRLLashktlspQM 351
Cdd:PRK07529 262 PLFHVNALLVTGLAPLARGAHVVLATPqgYRGPGVIANFWK--------IVERYRINFLSGVPTVYAALL--------QV 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 352 QAATSEATSpanLRVNISGSAALPTPVKAAWAALSGGNVlLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVD 431
Cdd:PRK07529 326 PVDGHDISS---LRYALCGAAPLPVEVFRRFEAATGVRI-VEGYGLTEATCVSSVNPPDGERRIGSVGLRLPYQRVRVVI 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 432 MDtgdviEPGHELDDHGRERVGEIQLRGPTVFREYwRNPDATAKEFVDDggdgrgRWFKTGDVAiRREPVGaagrnshrq 511
Cdd:PRK07529 402 LD-----DAGRYLRDCAVDEVGVLCIAGPNVFSGY-LEAAHNKGLWLED------GWLNTGDLG-RIDADG--------- 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 512 pwaqgpmYF-IQGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEM-- 588
Cdd:PRK07529 460 -------YFwLTGRAK-DLIIRGGHNIDPAAIEEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGASA--TEAELla 529
|
570 580 590 600
....*....|....*....|....*....|....*....|
gi 573989775 589 --RRALKSRLAgykIPQVMKIVNHIPRNAMGKINKKQLVK 626
Cdd:PRK07529 530 faRDHIAERAA---VPKHVRILDALPKTAVGKIFKPALRR 566
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
66-621 |
1.27e-31 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 129.55 E-value: 1.27e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVHALSGRRFTYGELLGDVAR-ARERLREAVDgkplTGERVAFLIENSYDYVVpeahsrdvVMFlA 144
Cdd:PRK08315 21 LLDRTAARYPDREALVYRDQGLRWTYREFNEEVDAlAKGLLALGIE----KGDRVGIWAPNVPEWVL--------TQF-A 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 145 VMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKF-----AAKAREV---LAT----DLQQK--P-----VYFELPK 205
Cdd:PRK08315 88 TAKIGAILVTINPAYRLSELEYALNQSGCKALIAADGFkdsdyVAMLYELapeLATcepgQLQSArlPelrrvIFLGDEK 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 206 HTGSSHGAK-----EAVSLADEDFSGAGL-------MLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVL 273
Cdd:PRK08315 168 HPGMLNFDEllalgRAVDDAELAARQATLdpddpinIQYTSGTTGFPKGATLTHRNILNNGYFIGEAMKLTEEDRLCIPV 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 274 PLHHIHGTINAVLTPLFAGSSVEFMFP-FNADAVWKrfaapflptngtattappisffTV----------VPTVYSRLLa 342
Cdd:PRK08315 248 PLYHCFGMVLGNLACVTHGATMVYPGEgFDPLATLA----------------------AVeeerctalygVPTMFIAEL- 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 343 SHKT-----LSpqmqaatseatspaNLRVNISGSAALPTPV-KAAwaalsggnvlLERYGMTEV----GMALSCGLAFAD 412
Cdd:PRK08315 305 DHPDfarfdLS--------------SLRTGIMAGSPCPIEVmKRV----------IDKMHMSEVtiayGMTETSPVSTQT 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 413 RVD-------ASVGWPLPSVEARLVDMDTGDVIEPGhelddhgreRVGEIQLRGPTVFREYWRNPDATAkEFVDDGGdgr 485
Cdd:PRK08315 361 RTDdplekrvTTVGRALPHLEVKIVDPETGETVPRG---------EQGELCTRGYSVMKGYWNDPEKTA-EAIDADG--- 427
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 486 grWFKTGDVAIRREpvgaagrnshrqpwaQGpmYF-IQGR-KtaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSG 563
Cdd:PRK08315 428 --WMHTGDLAVMDE---------------EG--YVnIVGRiK--DMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDE 486
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*...
gi 573989775 564 NWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINK 621
Cdd:PRK08315 487 KYGEEVCAWIILRPGATL--TEEDVRDFCRGKIAHYKIPRYIRFVDEFPMTVTGKIQK 542
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
229-626 |
1.81e-31 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 125.14 E-value: 1.81e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 LMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGtinavLTPLFAG--SSVEFMFPFNADAV 306
Cdd:cd17630 4 TVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWLLSLPLYHVGG-----LAILVRSllAGAELVLLERNQAL 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 307 WKRfaapflptngtaTTAPPISFFTVVPTVYSRLLASHKTlspqmqaatseATSPANLRVNISGSAALPTPvkAAWAALS 386
Cdd:cd17630 79 AED------------LAPPGVTHVSLVPTQLQRLLDSGQG-----------PAALKSLRAVLLGGAPIPPE--LLERAAD 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 387 GGNVLLERYGMTEVGmALSCGLAFADRVDASVGWPLPSVEARLVDMdtgdviepghelddhgrervGEIQLRGPTVFREY 466
Cdd:cd17630 134 RGIPLYTTYGMTETA-SQVATKRPDGFGRGGVGVLLPGRELRIVED--------------------GEIWVGGASLAMGY 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 467 WRNPdaTAKEFVDDGgdgrgrWFKTGDVAIrrepvgaagrnshrqpWAQGPMYFIQGRKTaDIIKTGGEKVSALEVEREL 546
Cdd:cd17630 193 LRGQ--LVPEFNEDG------WFTTKDLGE----------------LHADGRLTVLGRAD-NMIISGGENIQPEEIEAAL 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 547 LSLPQVAEAAVVAVPSGNWGQKVGAVIILDPdhavKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLVK 626
Cdd:cd17630 248 AAHPAVRDAFVVGVPDEELGQRPVAVIVGRG----PADPAELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRA 323
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
86-624 |
1.02e-30 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 125.28 E-value: 1.02e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 86 GRRFTYGELLGDVARARERLREAvdGKPLTGERVAFLIENSydyvvPEAhsrdVVMFLAVMAARAIAVPLSPPFPAPELQ 165
Cdd:cd05958 8 EREWTYRDLLALANRIANVLVGE--LGIVPGNRVLLRGSNS-----PEL----VACWFGIQKAGAIAVATMPLLRPKELA 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 166 YILDqsaasvlvaspkfaaKAREVLATdlqqkpvyfelpkhtgsshgAKEAVSLADEdfsgAGLMLYTSGTTNRPKGVLL 245
Cdd:cd05958 77 YILD---------------KARITVAL--------------------CAHALTASDD----ICILAFTSGTTGAPKATMH 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 246 P--EPVITAQC--RSLLQAweySPADRLLHVLPLHHIHGTINAVLTPLFAGSSVeFMFPfnadavwKRFAAPFLptnGTA 321
Cdd:cd05958 118 FhrDPLASADRyaVNVLRL---REDDRFVGSPPLAFTFGLGGVLLFPFGVGASG-VLLE-------EATPDLLL---SAI 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 322 TTAPPISFFTVvPTVYSRLLASHKTLSPQMqaatseatspANLRVNISGSAALPTPVKAAWAALSGGNvLLERYGMTEVG 401
Cdd:cd05958 184 ARYKPTVLFTA-PTAYRAMLAHPDAAGPDL----------SSLRKCVSAGEALPAALHRAWKEATGIP-IIDGIGSTEMF 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 402 MALSCGLAFADRVDASvGWPLPSVEARLVDmDTGDVIEPGhelddhgreRVGEIQLRGPTVfreYWRNPDATAKEFVDDG 481
Cdd:cd05958 252 HIFISARPGDARPGAT-GKPVPGYEAKVVD-DEGNPVPDG---------TIGRLAVRGPTG---CRYLADKRQRTYVQGG 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 482 gdgrgrWFKTGDvAIRREPVGaagrnshrqpwaqgpmYFIQGRKTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVP 561
Cdd:cd05958 318 ------WNITGD-TYSRDPDG----------------YFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHP 374
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 573989775 562 SGNWGQKVGAVIILDPDHAVKWTPL-EMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd05958 375 DESRGVVVKAFVVLRPGVIPGPVLArELQDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFAL 438
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
44-626 |
2.41e-30 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 128.15 E-value: 2.41e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 44 AHHRLRMLSTTTTTASTLPRLP----IFEAVAAHDPTSTAVVhaLSGRRFTYGELLGDVARARERLREAVDGkplTGERV 119
Cdd:PRK12316 1982 AGERQRILADWDRTPEAYPRGPgvhqRIAEQAARAPEAIAVV--FGDQHLSYAELDSRANRLAHRLRARGVG---PEVRV 2056
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 120 AFLIENSYDYVVPeahsrdvvmFLAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAK---AREVLATDLQQ 196
Cdd:PRK12316 2057 AIAAERSFELVVA---------LLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLLTQRHLLERlplPAGVARLPLDR 2127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 197 KPVYFELPKHtgsshgaKEAVSLADEDFSgagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLh 276
Cdd:PRK12316 2128 DAEWADYPDT-------APAVQLAGENLA---YVIYTSGSTGLPKGVAVSHGALVAHCQAAGERYELSPADCELQFMSF- 2196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 277 HIHGTINAVLTPLFAGSSVefmfPFNADAVWkrfaapfLPTNGTATTAPPISFFTVVPTVYSRLLASHktlspqmqaaTS 356
Cdd:PRK12316 2197 SFDGAHEQWFHPLLNGARV----LIRDDELW-------DPEQLYDEMERHGVTILDFPPVYLQQLAEH----------AE 2255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 357 EATSPANLRVNISGSAALPTPVKAAWAALSGGNVLLERYGMTEVgmALSCGLAFADRVDASVGWPLPSveARLVDMDTGD 436
Cdd:PRK12316 2256 RDGRPPAVRVYCFGGEAVPAASLRLAWEALRPVYLFNGYGPTEA--VVTPLLWKCRPQDPCGAAYVPI--GRALGNRRAY 2331
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 437 VIEPGHELDDHGreRVGEIQLRGPTVFREYWRNPDATAKEFVDDGGDGRG-RWFKTGDVAIRREP--VGAAGRNSHRqpw 513
Cdd:PRK12316 2332 ILDADLNLLAPG--MAGELYLGGEGLARGYLNRPGLTAERFVPDPFSASGeRLYRTGDLARYRADgvVEYLGRIDHQ--- 2406
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 514 aqgpmyfiqgrktadiIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIildPDHAVKWTPLEMRRALK 593
Cdd:PRK12316 2407 ----------------VKIRGFRIELGEIEARLQAHPAVREAVVVAQDGASGKQLVAYVV---PDDAAEDLLAELRAWLA 2467
|
570 580 590
....*....|....*....|....*....|...
gi 573989775 594 SRLAGYKIPQVMKIVNHIPRNAMGKINKKQLVK 626
Cdd:PRK12316 2468 ARLPAYMVPAHWVVLERLPLNPNGKLDRKALPK 2500
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
89-624 |
2.42e-30 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 123.99 E-value: 2.42e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 89 FTYGELLGDVARARERLREAVDGKpltGERVAFLIENsydyvVPEAhsrdVVMFLAVMAARAIAVPLSPPFPAPELQYIL 168
Cdd:cd05972 1 WSFRELKRESAKAANVLAKLGLRK---GDRVAVLLPR-----VPEL----WAVILAVIKLGAVYVPLTTLLGPKDIEYRL 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 169 DQSAASVLVASpkfaakarevlatdlqqkpvyfelpkhtgsshgakeavslaDEDFSgagLMLYTSGTTNRPKGVLLPEp 248
Cdd:cd05972 69 EAAGAKAIVTD-----------------------------------------AEDPA---LIYFTSGTTGLPKGVLHTH- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 249 vitaqcRSLLQAWEYSpadRLLHVLPLHHIHGTINAVLTPLFAGSSveFMFPFNADA-VWKRFAAPFLPTNGTAT-TAPP 326
Cdd:cd05972 104 ------SYPLGHIPTA---AYWLGLRPDDIHWNIADPGWAKGAWSS--FFGPWLLGAtVFVYEGPRFDAERILELlERYG 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 327 ISFFTVVPTVYSRLLAshktlspqmqaATSEATSPANLRVNISGSAALpTPVKAAWAALSGGNVLLERYGMTEVGMALSc 406
Cdd:cd05972 173 VTSFCGPPTAYRMLIK-----------QDLSSYKFSHLRLVVSAGEPL-NPEVIEWWRAATGLPIRDGYGQTETGLTVG- 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 407 GLAFADRVDASVGWPLPSVEARLVDmDTGDVIEPGHElddhgrervGEIQLRGPTV--FREYWRNPDATAKEFVDDggdg 484
Cdd:cd05972 240 NFPDMPVKPGSMGRPTPGYDVAIID-DDGRELPPGEE---------GDIAIKLPPPglFLGYVGDPEKTEASIRGD---- 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 485 rgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIqGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGN 564
Cdd:cd05972 306 ---YYLTGDRAYRDE---------------DGYFWFV-GRAD-DIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPV 365
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 573989775 565 WGQKVGAVIILDPDH-AVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd05972 366 RGEVVKAFVVLTSGYePSEELAEELQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVEL 426
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
66-624 |
3.05e-30 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 124.62 E-value: 3.05e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSydyvvPEAhsrdVVMFLAV 145
Cdd:cd12117 2 LFEEQAARTPDAVAVVYG--DRSLTYAELNERANRLARRLRAAGVGP---GDVVGVLAERS-----PEL----VVALLAV 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPvyfelpkhtGSSHGAKEAVSLADEDfS 225
Cdd:cd12117 68 LKAGAAYVPLDPELPAERLAFMLADAGAKVLLTDRSLAGRAGGLEVAVVIDEA---------LDAGPAGNPAVPVSPD-D 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 226 GAGLMlYTSGTTNRPKGVLLPEPVITAQCRSllQAW-EYSPADRLLHVLPLHhihgtinavltplFAGSSVEfmfpfnad 304
Cdd:cd12117 138 LAYVM-YTSGSTGRPKGVAVTHRGVVRLVKN--TNYvTLGPDDRVLQTSPLA-------------FDASTFE-------- 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 305 aVWkrfaAPFLpTNGTATTAPPISFFTvvPTVYSRLLASHKTLSPQM------QAATSEATSPANLRVNISGSAALPTP- 377
Cdd:cd12117 194 -IW----GALL-NGARLVLAPKGTLLD--PDALGALIAEEGVTVLWLtaalfnQLADEDPECFAGLRELLTGGEVVSPPh 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 378 VKAAWAALSGGnVLLERYGMTE-VGMALSCGLAFADRVDASV--GWPLPSVEARLVDmDTGDVIEPGhelddhgreRVGE 454
Cdd:cd12117 266 VRRVLAACPGL-RLVNGYGPTEnTTFTTSHVVTELDEVAGSIpiGRPIANTRVYVLD-EDGRPVPPG---------VPGE 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 455 IQLRGPTVFREYWRNPDATAKEFVDDGGDGRGRWFKTGDVAiRREPVGA---AGRNSHRqpwaqgpmyfiqgrktadiIK 531
Cdd:cd12117 335 LYVGGDGLALGYLNRPALTAERFVADPFGPGERLYRTGDLA-RWLPDGRlefLGRIDDQ-------------------VK 394
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 532 TGGEKVSALEVERELLSLPQVAEAAVVAVPsGNWGQK--VGAVIildPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVN 609
Cdd:cd12117 395 IRGFRIELGEIEAALRAHPGVREAVVVVRE-DAGGDKrlVAYVV---AEGAL--DAAELRAFLRERLPAYMVPAAFVVLD 468
|
570
....*....|....*
gi 573989775 610 HIPRNAMGKINKKQL 624
Cdd:cd12117 469 ELPLTANGKVDRRAL 483
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
84-624 |
3.67e-30 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 124.81 E-value: 3.67e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 84 LSG-RRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENsyDYVVPEAHsrdvvmfLAVMAARAIAVPLSPPFPAP 162
Cdd:PRK12406 6 ISGdRRRSFDELAQRAARAAGGLAALGVRP---GDCVALLMRN--DFAFFEAA-------YAAMRLGAYAVPVNWHFKPE 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 163 ELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVyfELPKHTGSSHGAKEAVSLADE---DFSG------------- 226
Cdd:PRK12406 74 EIAYILEDSGARVLIAHADLLHGLASALPAGVTVLSV--PTPPEIAAAYRISPALLTPPAgaiDWEGwlaqqepydgppv 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 227 --AGLMLYTSGTTNRPKGV--LLPEPVITA-QCRSLLQAWEYSPADRLLHVLPLHHihGTINAV-LTPLFAGSSVEFMFP 300
Cdd:PRK12406 152 pqPQSMIYTSGTTGHPKGVrrAAPTPEQAAaAEQMRALIYGLKPGIRALLTGPLYH--SAPNAYgLRAGRLGGVLVLQPR 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 301 FNADAvwkrfaapFLptngTATTAPPISFFTVVPTVYSRLLashktlspQMQAATSEATSPANLRVNISGSAALPTPVKA 380
Cdd:PRK12406 230 FDPEE--------LL----QLIERHRITHMHMVPTMFIRLL--------KLPEEVRAKYDVSSLRHVIHAAAPCPADVKR 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 381 A----WaalsgGNVLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDmDTGDVIEPGHelddhgrerVGEIQ 456
Cdd:PRK12406 290 AmiewW-----GPVIYEYYGSTESGAVTFATSEDALSHPGTVGKAAPGAELRFVD-EDGRPLPQGE---------IGEIY 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 457 LRGPTVFREYWRNPDATAKEFvddggdGRGRWFKTGDVAIrrepVGAAGRnshrqpwaqgpmYFIQGRKTaDIIKTGGEK 536
Cdd:PRK12406 355 SRIAGNPDFTYHNKPEKRAEI------DRGGFITSGDVGY----LDADGY------------LFLCDRKR-DMVISGGVN 411
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 537 VSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIilDPDHAVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAM 616
Cdd:PRK12406 412 IYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVV--EPQPGATLDEADIRAQLKARLAGYKVPKHIEIMAELPREDS 489
|
....*...
gi 573989775 617 GKINKKQL 624
Cdd:PRK12406 490 GKIFKRRL 497
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
66-626 |
2.05e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 125.27 E-value: 2.05e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVhaLSGRRFTYGELLGDVARARERLREAVDGkplTGERVAFLIENSYDYVVPeahsrdvvmFLAV 145
Cdd:PRK12467 517 LIEAQARQHPERPALV--FGEQVLSYAELNRQANRLAHVLIAAGVG---PDVLVGIAVERSIEMVVG---------LLAV 582
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAakAREVLATDLQQKPVYFELPKHTGSShGAKEAVSLADEDFS 225
Cdd:PRK12467 583 LKAGGAYVPLDPEYPQDRLAYMLDDSGVRLLLTQSHLL--AQLPVPAGLRSLCLDEPADLLCGYS-GHNPEVALDPDNLA 659
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 226 gagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLhHIHGTINAVLTPLFAGSSVEFMFPfnaDA 305
Cdd:PRK12467 660 ---YVIYTSGSTGQPKGVAISHGALANYVCVIAERLQLAADDSMLMVSTF-AFDLGVTELFGALASGATLHLLPP---DC 732
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 306 VWKrfAAPFLptngTATTAPPISFFTVVPTVYSRLLashktlspqmQAATSEATSPanLRVNISGSAALPTPVKAAWAAL 385
Cdd:PRK12467 733 ARD--AEAFA----ALMADQGVTVLKIVPSHLQALL----------QASRVALPRP--QRALVCGGEALQVDLLARVRAL 794
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 386 SGGNVLLERYGMTE--VGMA-LSCGLAFADRVDASVGWPLPSVEARLVDmdtgdviepgHELDDHGRERVGEIQLRGPTV 462
Cdd:PRK12467 795 GPGARLINHYGPTEttVGVStYELSDEERDFGNVPIGQPLANLGLYILD----------HYLNPVPVGVVGELYIGGAGL 864
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 463 FREYWRNPDATAKEFVDD--GGDGrGRWFKTGDVAIRRepvgaagrnshrqpwAQGPMYFIqGRkTADIIKTGGEKVSAL 540
Cdd:PRK12467 865 ARGYHRRPALTAERFVPDpfGADG-GRLYRTGDLARYR---------------ADGVIEYL-GR-MDHQVKIRGFRIELG 926
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 541 EVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVK--WTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGK 618
Cdd:PRK12467 927 EIEARLLAQPGVREAVVLAQPGDAGLQLVAYLVPAAVADGAEhqATRDELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGK 1006
|
....*...
gi 573989775 619 INKKQLVK 626
Cdd:PRK12467 1007 LDRKALPK 1014
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
75-624 |
2.74e-29 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 122.36 E-value: 2.74e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHAlsGRRFTYGELLgdvARAReRLREAVDGKPLT-GERVAFLIENsydyvVP---EAHsrdvvmfLAVMAARA 150
Cdd:PRK08162 32 PDRPAVIHG--DRRRTWAETY---ARCR-RLASALARRGIGrGDTVAVLLPN-----IPamvEAH-------FGVPMAGA 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 151 IAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKP--VYFELPKHTGSSH-GAKE---AVSLADEDF 224
Cdd:PRK08162 94 VLNTLNTRLDAASIAFMLRHGEAKVLIVDTEFAEVAREALALLPGPKPlvIDVDDPEYPGGRFiGALDyeaFLASGDPDF 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 225 SG--------AGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHG-----TINAVltplfA 291
Cdd:PRK08162 174 AWtlpadewdAIALNYTSGTTGNPKGVVYHHRGAYLNALSNILAWGMPKHPVYLWTLPMFHCNGwcfpwTVAAR-----A 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 292 GSSVeFMFPFNADAVWkrfaapflptngTATTAPPISFFTVVPTVYSRLLAshktlspqmqaATSEATSPANLRVN-ISG 370
Cdd:PRK08162 249 GTNV-CLRKVDPKLIF------------DLIREHGVTHYCGAPIVLSALIN-----------APAEWRAGIDHPVHaMVA 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 371 SAALPTPVKAAWAALsgGNVLLERYGMTEV-GMALSC-------GLAFADRVD--ASVGWPLPSVEA-RLVDMDTG-DVI 438
Cdd:PRK08162 305 GAAPPAAVIAKMEEI--GFDLTHVYGLTETyGPATVCawqpewdALPLDERAQlkARQGVRYPLQEGvTVLDPDTMqPVP 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 439 EPGhelddhgrERVGEIQLRGPTVFREYWRNPDATAKEFvddggdgRGRWFKTGDVAIRrepvgaagrnsHRQPWAQgpm 518
Cdd:PRK08162 383 ADG--------ETIGEIMFRGNIVMKGYLKNPKATEEAF-------AGGWFHTGDLAVL-----------HPDGYIK--- 433
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 519 yfIQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAG 598
Cdd:PRK08162 434 --IKDR-SKDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASA--TEEEIIAHCREHLAG 508
|
570 580
....*....|....*....|....*.
gi 573989775 599 YKIPQVMkIVNHIPRNAMGKINKKQL 624
Cdd:PRK08162 509 FKVPKAV-VFGELPKTSTGKIQKFVL 533
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
75-624 |
3.70e-29 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 120.44 E-value: 3.70e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVPEahsrdvvmfLAVMAARAIAVP 154
Cdd:cd17652 1 PDAPAVVFG--DETLTYAELNARANRLARLLAARGVGP---ERLVALALPRSAELVVAI---------LAVLKAGAAYLP 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 155 LSPPFPAPELQYILDQSAASVLVASPkfaakarevlatdlqQKPVYfelpkhtgsshgakeavsladedfsgaglMLYTS 234
Cdd:cd17652 67 LDPAYPAERIAYMLADARPALLLTTP---------------DNLAY-----------------------------VIYTS 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 235 GTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHvlplhhihgtinavltplFAGSSvefmfpFNAdAVWKRFAApf 314
Cdd:cd17652 103 GSTGRPKGVVVTHRGLANLAAAQIAAFDVGPGSRVLQ------------------FASPS------FDA-SVWELLMA-- 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 315 LPTNGTATTAPPISffTVVPTVYSRLLA----SHKTLSPQMQAATSEATSPAnLRVNISGSAALPTPVKAAWAAlsgGNV 390
Cdd:cd17652 156 LLAGATLVLAPAEE--LLPGEPLADLLRehriTHVTLPPAALAALPPDDLPD-LRTLVVAGEACPAELVDRWAP---GRR 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 391 LLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDmDTGDVIEPGhelddhgreRVGEIQLRGPTVFREYWRNP 470
Cdd:cd17652 230 MINAYGPTETTVCATMAGPLPGGGVPPIGRPVPGTRVYVLD-ARLRPVPPG---------VPGELYIAGAGLARGYLNRP 299
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 471 DATAKEFVDD--GGDGrGRWFKTGDVAIRRepvgaagrnshrqpwAQGPMYFIqGRkTADIIKTGGEKVSALEVERELLS 548
Cdd:cd17652 300 GLTAERFVADpfGAPG-SRMYRTGDLARWR---------------ADGQLEFL-GR-ADDQVKIRGFRIELGEVEAALTE 361
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 573989775 549 LPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd17652 362 HPGVAEAVVVVRDDRPGDKRLVAYVVPAPGAAP--TAAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
75-624 |
3.70e-29 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 120.88 E-value: 3.70e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGkplTGERVAFLIENSYDYVVPeahsrdvvmFLAVMAARAIAVP 154
Cdd:cd17643 1 PEAVAVVDE--DRRLTYGELDARANRLARTLRAEGVG---PGDRVALALPRSAELIVA---------LLAILKAGGAYVP 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 155 LSPPFPAPELQYILDQSAASVLVASPkfaakarevlatdlqQKPVYfelpkhtgsshgakeavsladedfsgaglMLYTS 234
Cdd:cd17643 67 IDPAYPVERIAFILADSGPSLLLTDP---------------DDLAY-----------------------------VIYTS 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 235 GTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRllhVLPLHHihgtinavltplfagssveFMFPFnadAVWKRFAApf 314
Cdd:cd17643 103 GSTGRPKGVVVSHANVLALFAATQRWFGFNEDDV---WTLFHS-------------------YAFDF---SVWEIWGA-- 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 315 LPTNGT------ATTAPPISFFTVV-----------PTVYSRLlashktlspqMQAATSEATSPANLRVNISGSAALPTP 377
Cdd:cd17643 156 LLHGGRlvvvpyEVARSPEDFARLLrdegvtvlnqtPSAFYQL----------VEAADRDGRDPLALRYVIFGGEALEAA 225
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 378 VKAAWAALSGGN--VLLERYGMTEVGMALSCGL---AFADRVDASV-GWPLPSVEARLVDMDTGDViEPGhelddhgreR 451
Cdd:cd17643 226 MLRPWAGRFGLDrpQLVNMYGITETTVHVTFRPldaADLPAAAASPiGRPLPGLRVYVLDADGRPV-PPG---------V 295
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 452 VGEIQLRGPTVFREYWRNPDATAKEFVDD--GGDGRgRWFKTGDVAiRREPVGA---AGRNSHRqpwaqgpmyfiqgrkt 526
Cdd:cd17643 296 VGELYVSGAGVARGYLGRPELTAERFVANpfGGPGS-RMYRTGDLA-RRLPDGEleyLGRADEQ---------------- 357
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 527 adiIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMK 606
Cdd:cd17643 358 ---VKIRGFRIELGEIEAALATHPSVRDAAVIVREDEPGDTRLVAYVVADDGAAA--DIAELRALLKELLPDYMVPARYV 432
|
570
....*....|....*...
gi 573989775 607 IVNHIPRNAMGKINKKQL 624
Cdd:cd17643 433 PLDALPLTVNGKLDRAAL 450
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
229-617 |
8.51e-29 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 117.40 E-value: 8.51e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 LMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIhGTINAVLTPLFAGSSVEFMFPFNADAVwk 308
Cdd:cd17636 4 LAIYTAAFSGRPNGALLSHQALLAQALVLAVLQAIDEGTVFLNSGPLFHI-GTLMFTLATFHAGGTNVFVRRVDAEEV-- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 309 rfaapflptngtattappisfftvvptvySRLLASHKT----LSPQMQAATSE--ATSPANLR-----VNISGSAALPTP 377
Cdd:cd17636 81 -----------------------------LELIEAERCthafLLPPTIDQIVElnADGLYDLSslrssPAAPEWNDMATV 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 378 VKAAWAALSGGnvllerYGMTEVgMALSCGLAFADRVDASVGWPLPSVEARLVDMDTGDViePGHElddhgrerVGEIQL 457
Cdd:cd17636 132 DTSPWGRKPGG------YGQTEV-MGLATFAALGGGAIGGAGRPSPLVQVRILDEDGREV--PDGE--------VGEIVA 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 458 RGPTVFREYWRNPDATAKEFvddggdgRGRWFKTGDVAiRREPVGAagrnshrqpwaqgpMYFIqGRKTAdIIKTGGEKV 537
Cdd:cd17636 195 RGPTVMAGYWNRPEVNARRT-------RGGWHHTNDLG-RREPDGS--------------LSFV-GPKTR-MIKSGAENI 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 538 SALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMG 617
Cdd:cd17636 251 YPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPGASV--TEAELIEHCRARIASYKKPKSVEFADALPRTAGG 328
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
116-624 |
8.62e-29 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 121.31 E-value: 8.62e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 116 GERVAFLIENSYDYvvPeahsrdVVMFlAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAA---------KA 186
Cdd:PRK08974 74 GDRVALMMPNLLQY--P------IALF-GILRAGMIVVNVNPLYTPRELEHQLNDSGAKAIVIVSNFAHtlekvvfktPV 144
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 187 REVLATDLQQ--------------KPVYFELPK-HTGSSHGAKEAVS-----------LADEDFSgagLMLYTSGTTNRP 240
Cdd:PRK08974 145 KHVILTRMGDqlstakgtlvnfvvKYIKRLVPKyHLPDAISFRSALHkgrrmqyvkpeLVPEDLA---FLQYTGGTTGVA 221
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 241 KGVLLPEPVITAqcrSLLQA-WEYSPadrLLHV--------LPLHHIHG-TINAVLTPLFAGSSVEFMFPFNADAVWKRF 310
Cdd:PRK08974 222 KGAMLTHRNMLA---NLEQAkAAYGP---LLHPgkelvvtaLPLYHIFAlTVNCLLFIELGGQNLLITNPRDIPGFVKEL 295
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 311 AA-PFlptngTATTAppisfftvVPTVYSRLLASH--KTLSPqmqaatseatspANLRVNISGSAALPTPVKAAWAALSG 387
Cdd:PRK08974 296 KKyPF-----TAITG--------VNTLFNALLNNEefQELDF------------SSLKLSVGGGMAVQQAVAERWVKLTG 350
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 388 GNvLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDmDTGDVIEPGhelddhgreRVGEIQLRGPTVFREYW 467
Cdd:PRK08974 351 QY-LLEGYGLTECSPLVSVNPYDLDYYSGSIGLPVPSTEIKLVD-DDGNEVPPG---------EPGELWVKGPQVMLGYW 419
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 468 RNPDATAkEFVDDGgdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIQGRKtaDIIKTGGEKVSALEVERELL 547
Cdd:PRK08974 420 QRPEATD-EVIKDG------WLATGDIAVMDE---------------EGFLRIVDRKK--DMILVSGFNVYPNEIEDVVM 475
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 573989775 548 SLPQVAEAAVVAVPSGNWGQKVGA-VIILDPdhavKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK08974 476 LHPKVLEVAAVGVPSEVSGEAVKIfVVKKDP----SLTEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRREL 549
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
68-626 |
1.03e-28 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 120.62 E-value: 1.03e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 68 EAVAAHDPTSTAVVHalSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMA 147
Cdd:PRK06164 17 DAHARARPDAVALID--EDRPLSRAELRALVDRLAAWLAAQGVRR---GDRVAVWLPNCIEWVV---------LFLACAR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 148 ARAIAVPLSPPFPAPELQYILDQSAASVLVASPKF---------AAKAREVL-----------ATDLQQKPVYFELPKHT 207
Cdd:PRK06164 83 LGATVIAVNTRYRSHEVAHILGRGRARWLVVWPGFkgidfaailAAVPPDALpplraiavvddAADATPAPAPGARVQLF 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 208 GSSHGAKEAVSLADEDFSGAGLMLYT-SGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGtINAVL 286
Cdd:PRK06164 163 ALPDPAPPAAAGERAADPDAGALLFTtSGTTSGPKLVLHRQATLLRHARAIARAYGYDPGAVLLAALPFCGVFG-FSTLL 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 287 TPLFAGSSVEFMFPFNAdavwkrfaapflptngtATTAppisfftvvptvysRLLASHKT--------LSPQMQAATSEA 358
Cdd:PRK06164 242 GALAGGAPLVCEPVFDA-----------------ARTA--------------RALRRHRVthtfgndeMLRRILDTAGER 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 359 TSPANLRVniSGSAA-LPTPVKAAWAALSGGNVLLERYGMTEVGMALSCG---LAFADRVDASvGWPL-PSVEARLVDMD 433
Cdd:PRK06164 291 ADFPSARL--FGFASfAPALGELAALARARGVPLTGLYGSSEVQALVALQpatDPVSVRIEGG-GRPAsPEARVRARDPQ 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 434 TGDVIEPGHElddhgrervGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRRepvgaagrnshrqpw 513
Cdd:PRK06164 368 DGALLPDGES---------GEIEIRAPSLMRGYLDNPDATARALTDDG------YFRTGDLGYTR--------------- 417
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 514 aqGPMYFIQGRKTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIildPDHAVKWTPLEMRRALK 593
Cdd:PRK06164 418 --GDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGATRDGKTVPVAFVI---PTDGASPDEAGLMAACR 492
|
570 580 590
....*....|....*....|....*....|...
gi 573989775 594 SRLAGYKIPQVMKIVNHIPRNAMGKINKKQLVK 626
Cdd:PRK06164 493 EALAGFKVPARVQVVEAFPVTESANGAKIQKHR 525
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
86-624 |
1.10e-28 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 121.02 E-value: 1.10e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 86 GRRFTYGELL---GDVArarERLREAVDGKPltGERVAFLIENSYDYvvpeahsrDVVMFLAvMAARAIAVPLSPPFPAP 162
Cdd:PRK05677 47 GKTLTYGELYklsGAFA---AWLQQHTDLKP--GDRIAVQLPNVLQY--------PVAVFGA-MRAGLIVVNTNPLYTAR 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 163 ELQYILDQSAASVLVASPKFAAKAREVLA-TDLQQKPVY---------------------------FELPKHTG----SS 210
Cdd:PRK05677 113 EMEHQFNDSGAKALVCLANMAHLAEKVLPkTGVKHVIVTevadmlpplkrllinavvkhvkkmvpaYHLPQAVKfndaLA 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 211 HGAKEAVSLADEDFSGAGLMLYTSGTTNRPKGVLLPEPVITA---QCRSLLQAWEYSPADRLLHVLPLHHIHG-TINAVL 286
Cdd:PRK05677 193 KGAGQPVTEANPQADDVAVLQYTGGTTGVAKGAMLTHRNLVAnmlQCRALMGSNLNEGCEILIAPLPLYHIYAfTFHCMA 272
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 287 TPLFAGSSVEFMFPFNADAVWK-----RFAApFLPTNgtattappisfftvvpTVYSRLLASHKTLSPQMQAatseatsp 361
Cdd:PRK05677 273 MMLIGNHNILISNPRDLPAMVKelgkwKFSG-FVGLN----------------TLFVALCNNEAFRKLDFSA-------- 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 362 anLRVNISGSAALPTPVKAAWAALSGGNVLlERYGMTEVGMALSCGLAFADRVdASVGWPLPSVEARLVDMDtgdviepG 441
Cdd:PRK05677 328 --LKLTLSGGMALQLATAERWKEVTGCAIC-EGYGMTETSPVVSVNPSQAIQV-GTIGIPVPSTLCKVIDDD-------G 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 442 HELddhGRERVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFI 521
Cdd:PRK05677 397 NEL---PLGEVGELCVKGPQVMKGYWQRPEATDEILDSDG------WLKTGDIALIQE---------------DGYMRIV 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 522 QGRKtaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKI 601
Cdd:PRK05677 453 DRKK--DMILVSGFNVYPNELEDVLAALPGVLQCAAIGVPDEKSGEAIKVFVVVKPGETL--TKEQVMEHMRANLTGYKV 528
|
570 580
....*....|....*....|...
gi 573989775 602 PQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK05677 529 PKAVEFRDELPTTNVGKILRREL 551
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
78-543 |
1.23e-28 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 120.46 E-value: 1.23e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 78 TAVVHALSGRRFTYGELLGDVARARERLREAvdgKPLTGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLSP 157
Cdd:cd05906 29 TYIDADGSEEFQSYQDLLEDARRLAAGLRQL---GLRPGDSVILQFDDNEDFIP---------AFWACVLAGFVPAPLTV 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 158 P----FPAPELQYI------LDQSaasVLVASPKFAAKAREVLAtdLQQKPVYFELPKHTGSSHGAKEAVSLADEDfsGA 227
Cdd:cd05906 97 PptydEPNARLRKLrhiwqlLGSP---VVLTDAELVAEFAGLET--LSGLPGIRVLSIEELLDTAADHDLPQSRPD--DL 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 228 GLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSvefmfPFNAdavw 307
Cdd:cd05906 170 ALLMLTSGSTGFPKAVPLTHRNILARSAGKIQHNGLTPQDVFLNWVPLDHVGGLVELHLRAVYLGCQ-----QVHV---- 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 308 krfaapflptngtattapPISFFTVVPTVYSRLLASHK---TLSPQM------QAATSEATSPANL---RVNISGSAALP 375
Cdd:cd05906 241 ------------------PTEEILADPLRWLDLIDRYRvtiTWAPNFafallnDLLEEIEDGTWDLsslRYLVNAGEAVV 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 376 TPVKAAWAALsggnvlLERY-----------GMTEV--GMALSCGLAFADRVDA----SVGWPLPSVEARLVDmDTGDVI 438
Cdd:cd05906 303 AKTIRRLLRL------LEPYglppdairpafGMTETcsGVIYSRSFPTYDHSQAlefvSLGRPIPGVSMRIVD-DEGQLL 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 439 EpghelddhgRERVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaagrnshrqpwaqGPM 518
Cdd:cd05906 376 P---------EGEVGRLQVRGPVVTKGYYNNPEANAEAFTEDG------WFRTGDLGFLDN----------------GNL 424
|
490 500
....*....|....*....|....*
gi 573989775 519 YfIQGRkTADIIKTGGEKVSALEVE 543
Cdd:cd05906 425 T-ITGR-TKDTIIVNGVNYYSHEIE 447
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
225-621 |
1.84e-28 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 116.59 E-value: 1.84e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 225 SGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQA-WEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNA 303
Cdd:cd17635 1 EDPLAVIFTSGTTGEPKAVLLANKTFFAVPDILQKEgLNWVVGDVTYLPLPATHIGGLWWILTCLIHGGLCVTGGENTTY 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 304 DAVWKRfaapfLPTNGTATTAppisfftVVPTVYSRLLASHKTLSpqmqaatseATSPaNLRVNISGSaALPTPVKAAWA 383
Cdd:cd17635 81 KSLFKI-----LTTNAVTTTC-------LVPTLLSKLVSELKSAN---------ATVP-SLRLIGYGG-SRAIAADVRFI 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 384 ALSGGNVLLERYGMTEVGMAlsCGLAFA-DRVDA-SVGWPLPSVEARLVDMDTGDVIEPGHelddhgrervGEIQLRGPT 461
Cdd:cd17635 138 EATGLTNTAQVYGLSETGTA--LCLPTDdDSIEInAVGRPYPGVDVYLAATDGIAGPSASF----------GTIWIKSPA 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 462 VFREYWRNPDATAKEFVDDggdgrgrWFKTGDVAIRREpvgaagrnshrqpwaqGPMYFIQGRKTADIIKtGGEKVSALE 541
Cdd:cd17635 206 NMLGYWNNPERTAEVLIDG-------WVNTGDLGERRE----------------DGFLFITGRSSESINC-GGVKIAPDE 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 542 VERELLSLPQVAEAAVVAVPSGNWGQKVGAVIIldpdhAVKWTPLEMRRALKSRLAG----YKIPQVMKIVNHIPRNAMG 617
Cdd:cd17635 262 VERIAEGVSGVQECACYEISDEEFGELVGLAVV-----ASAELDENAIRALKHTIRRelepYARPSTIVIVTDIPRTQSG 336
|
....
gi 573989775 618 KINK 621
Cdd:cd17635 337 KVKR 340
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
67-624 |
1.91e-28 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 119.35 E-value: 1.91e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 67 FEAVAAHDPTSTAVVhaLSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVPeahsrdvvmFLAVM 146
Cdd:cd17655 3 FEEQAEKTPDHTAVV--FEDQTLTYRELNERANQLARTLREKGVGP---DTIVGIMAERSLEMIVG---------ILGIL 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 147 AARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAK---AREVLATDLQQkpvyfelpkhtgSSHGAKEAVSlADED 223
Cdd:cd17655 69 KAGGAYLPIDPDYPEERIQYILEDSGADILLTQSHLQPPiafIGLIDLLDEDT------------IYHEESENLE-PVSK 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 224 FSGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLhHIHGTINAVLTPLFAGSSVeFMFPFNA 303
Cdd:cd17655 136 SDDLAYVIYTSGSTGKPKGVMIEHRGVVNLVEWANKVIYQGEHLRVALFASI-SFDASVTEIFASLLSGNTL-YIVRKET 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 304 DAVWKRFAAPFLPTNGTATTAPPisfftvvptvysrllaSHKTLSPQMQAATSEatspaNLRVNISGSAALPTPVKAAWA 383
Cdd:cd17655 214 VLDGQALTQYIRQNRITIIDLTP----------------AHLKLLDAADDSEGL-----SLKHLIVGGEALSTELAKKII 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 384 ALSGGNV-LLERYGMTE--VG-MALSCGLAFADRVDASVGWPLPSVEARLVdmDTGDVIEPGHElddhgrerVGEIQLRG 459
Cdd:cd17655 273 ELFGTNPtITNAYGPTEttVDaSIYQYEPETDQQVSVPIGKPLGNTRIYIL--DQYGRPQPVGV--------AGELYIGG 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 460 PTVFREYWRNPDATAKEFVDDGGDGRGRWFKTGDVAiRREPVGA---AGRNSHRqpwaqgpmyfiqgrktadiIKTGGEK 536
Cdd:cd17655 343 EGVARGYLNRPELTAEKFVDDPFVPGERMYRTGDLA-RWLPDGNiefLGRIDHQ-------------------VKIRGYR 402
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 537 VSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDhavkWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAM 616
Cdd:cd17655 403 IELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIVSEKE----LPVAQLREFLARELPDYMIPSYFIKLDEIPLTPN 478
|
....*...
gi 573989775 617 GKINKKQL 624
Cdd:cd17655 479 GKVDRKAL 486
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
230-621 |
3.08e-28 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 115.68 E-value: 3.08e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 230 MLYTSGTTNRPKGVLLPEpvitAQCRSLLQAW----EYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADA 305
Cdd:cd17638 5 IMFTSGTTGRSKGVMCAH----RQTLRAAAAWadcaDLTEDDRYLIINPFFHTFGYKAGIVACLLTGATVVPVAVFDVDA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 306 VWKRFAAPflptngtattapPISFFTVVPTVYSRLLAShktlsPQMQAAtseatSPANLRVNISGSAALPTP-VKAAWAA 384
Cdd:cd17638 81 ILEAIERE------------RITVLPGPPTLFQSLLDH-----PGRKKF-----DLSSLRAAVTGAATVPVElVRRMRSE 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 385 LSGGNVLLErYGMTEVGMALSC--GLAFADrVDASVGWPLPSVEARLVDMdtgdviepghelddhgrervGEIQLRGPTV 462
Cdd:cd17638 139 LGFETVLTA-YGLTEAGVATMCrpGDDAET-VATTCGRACPGFEVRIADD--------------------GEVLVRGYNV 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 463 FREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIrrepVGAAGRNShrqpwaqgpmyfIQGRKTaDIIKTGGEKVSALEV 542
Cdd:cd17638 197 MQGYLDDPEATAEAIDADG------WLHTGDVGE----LDERGYLR------------ITDRLK-DMYIVGGFNVYPAEV 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 543 ERELLSLPQVAEAAVVAVPSGNWGQKVGAVII------LDPDHAVKWTplemrralKSRLAGYKIPQVMKIVNHIPRNAM 616
Cdd:cd17638 254 EGALAEHPGVAQVAVIGVPDERMGEVGKAFVVarpgvtLTEEDVIAWC--------RERLANYKVPRFVRFLDELPRNAS 325
|
....*
gi 573989775 617 GKINK 621
Cdd:cd17638 326 GKVMK 330
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
67-498 |
4.32e-28 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 120.73 E-value: 4.32e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 67 FEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAvdG-KPltGERVAFLIENSYDYVVpeahsrdvvMFLAV 145
Cdd:COG1020 482 FEAQAARTPDAVAVVFG--DQSLTYAELNARANRLAHHLRAL--GvGP--GDLVGVCLERSLEMVV---------ALLAV 546
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAK----AREVLATDlqqkpvyfELPKHTGSSHGAKEAVSLAD 221
Cdd:COG1020 547 LKAGAAYVPLDPAYPAERLAYMLEDAGARLVLTQSALAARlpelGVPVLALD--------ALALAAEPATNPPVPVTPDD 618
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 222 edfsgagL--MLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHiHGTINAVLTPLFAGSSVEFmf 299
Cdd:COG1020 619 -------LayVIYTSGSTGRPKGVMVEHRALVNLLAWMQRRYGLGPGDRVLQFASLSF-DASVWEIFGALLSGATLVL-- 688
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 300 pfnADAVWKRFAAPFLptngTATTAPPISFFTVVPTVYSRLLASHktlspqmqaatseATSPANLRVNISGSAALPTPVK 379
Cdd:COG1020 689 ---APPEARRDPAALA----ELLARHRVTVLNLTPSLLRALLDAA-------------PEALPSLRLVLVGGEALPPELV 748
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 380 AAWAALSGGNVLLERYGMTEVGM-ALSCGLAFADRVDASV--GWPLPSVEARLvdmdtgdviepgheLDDHGRER----V 452
Cdd:COG1020 749 RRWRARLPGARLVNLYGPTETTVdSTYYEVTPPDADGGSVpiGRPIANTRVYV--------------LDAHLQPVpvgvP 814
|
410 420 430 440
....*....|....*....|....*....|....*....|....*..
gi 573989775 453 GEIQLRGPTVFREYWRNPDATAKEFVDD-GGDGRGRWFKTGDVAIRR 498
Cdd:COG1020 815 GELYIGGAGLARGYLNRPELTAERFVADpFGFPGARLYRTGDLARWL 861
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
67-624 |
9.08e-28 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 116.65 E-value: 9.08e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 67 FEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGkplTGERVAFLIENSYDYVVpeahsrdvvMFLAVM 146
Cdd:cd12115 5 VEAQAARTPDAIALVCG--DESLTYAELNRRANRLAARLRAAGVG---PESRVGVCLERTPDLVV---------ALLAVL 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 147 AARAIAVPLSPPFPAPELQYILDQSAASVLVaspkfaakarevlaTDLQQkPVYfelpkhtgsshgakeavsladedfsg 226
Cdd:cd12115 71 KAGAAYVPLDPAYPPERLRFILEDAQARLVL--------------TDPDD-LAY-------------------------- 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 227 aglMLYTSGTTNRPKGVLLPEPVITAqcrsLLQ-AWEYSPADRLLHVLPLHHI--HGTINAVLTPLFAGSSVefmfpFNA 303
Cdd:cd12115 110 ---VIYTSGSTGRPKGVAIEHRNAAA----FLQwAAAAFSAEELAGVLASTSIcfDLSVFELFGPLATGGKV-----VLA 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 304 DAVWKRFAAPflptngtatTAPPISFFTVVPTVYSRLLAsHKTLspqmqaatseatsPANLR-VNISGSAaLPTPVKAAW 382
Cdd:cd12115 178 DNVLALPDLP---------AAAEVTLINTVPSAAAELLR-HDAL-------------PASVRvVNLAGEP-LPRDLVQRL 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 383 AALSGGNVLLERYGMTE-VGMALSCGLAFADRVDASVGWPLPSVEArlvdmdtgdviepgHELDDHGRER----VGEIQL 457
Cdd:cd12115 234 YARLQVERVVNLYGPSEdTTYSTVAPVPPGASGEVSIGRPLANTQA--------------YVLDRALQPVplgvPGELYI 299
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 458 RGPTVFREYWRNPDATAKEFVDDGGDGRGRWFKTGDVAIRRepvgaagrnshrqpwAQGPMYFIqGRKTaDIIKTGGEKV 537
Cdd:cd12115 300 GGAGVARGYLGRPGLTAERFLPDPFGPGARLYRTGDLVRWR---------------PDGLLEFL-GRAD-NQVKVRGFRI 362
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 538 SALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMG 617
Cdd:cd12115 363 ELGEIEAALRSIPGVREAVVVAIGDAAGERRLVAYIVAEPGAAG--LVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNG 440
|
....*..
gi 573989775 618 KINKKQL 624
Cdd:cd12115 441 KIDRSAL 447
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
88-625 |
1.05e-27 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 116.38 E-value: 1.05e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 88 RFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDyvVPEAHsrdvvmfLAVMAARAIAVPLSPPFPAPELQYI 167
Cdd:cd05971 6 KVTFKELKTASNRFANVLKEIGLEK---GDRVGVFLSQGPE--CAIAH-------IAILRSGAIAVPLFALFGPEALEYR 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 168 LDQSAASVLVaspkfaakarevlaTDLQQKPvyfelpkhtgsshgakeavsladedfsgaGLMLYTSGTTNRPKGVLLPE 247
Cdd:cd05971 74 LSNSGASALV--------------TDGSDDP-----------------------------ALIIYTSGTTGPPKGALHAH 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 248 PVITAQCRSLLQAWEYSPADRLLHVLPLHH--IHGTINAVLTPLFAGSSV--EFMFPFNADAVWKrfaapFLPTNGTATT 323
Cdd:cd05971 111 RVLLGHLPGVQFPFNLFPRDGDLYWTPADWawIGGLLDVLLPSLYFGVPVlaHRMTKFDPKAALD-----LMSRYGVTTA 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 324 APPisfftvvPTVYsRLLASHKtlsPQMQAAtseatsPANLRVNISGSAALPTpVKAAWAALSGGNVLLERYGMTEVGMA 403
Cdd:cd05971 186 FLP-------PTAL-KMMRQQG---EQLKHA------QVKLRAIATGGESLGE-ELLGWAREQFGVEVNEFYGQTECNLV 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 404 LSCGLAFADRVDASVGWPLPSVEARLVDmDTGDVIEPGhelddhgreRVGEIQLR--GPTVFREYWRNPDATAKEFVddg 481
Cdd:cd05971 248 IGNCSALFPIKPGSMGKPIPGHRVAIVD-DNGTPLPPG---------EVGEIAVElpDPVAFLGYWNNPSATEKKMA--- 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 482 gdgrGRWFKTGDVAIRRepvgaagrnshrqpwAQGPMYFIqGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVP 561
Cdd:cd05971 315 ----GDWLLTGDLGRKD---------------SDGYFWYV-GRDD-DVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIP 373
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 573989775 562 SGNWGQKVGAVIILDPDHaVKWTPL--EMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLV 625
Cdd:cd05971 374 DPIRGEIVKAFVVLNPGE-TPSDALarEIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELR 438
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
71-625 |
1.36e-27 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 117.10 E-value: 1.36e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHALSGRRFTYGELlgdVARARE--RLREAVDGKPltGERVAFLIENsydyvvpeaHSRdvvMFLAVMAA 148
Cdd:PRK13391 7 AQTTPDKPAVIMASTGEVVTYREL---DERSNRlaHLFRSLGLKR--GDHVAIFMEN---------NLR---YLEVCWAA 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 149 RAIA---VPLSPPFPAPELQYILDQSAASVLVASpkfaAKAREVLATDLQQKP------VYF---ELPKHTGSSHGAKE- 215
Cdd:PRK13391 70 ERSGlyyTCVNSHLTPAEAAYIVDDSGARALITS----AAKLDVARALLKQCPgvrhrlVLDgdgELEGFVGYAEAVAGl 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 216 -AVSLADEdfSGAGLMLYTSGTTNRPKGVL--LPE------PVITAQCRSLlqaWEYSPADRLLHVLPLHHiHGTINAVL 286
Cdd:PRK13391 146 pATPIADE--SLGTDMLYSSGTTGRPKGIKrpLPEqppdtpLPLTAFLQRL---WGFRSDMVYLSPAPLYH-SAPQRAVM 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 287 TPLFAGSSVEFMFPFNAD---AVWKRFAapflptngtattappISFFTVVPTVYSRLLashktlspQMQAATSEATSPAN 363
Cdd:PRK13391 220 LVIRLGGTVIVMEHFDAEqylALIEEYG---------------VTHTQLVPTMFSRML--------KLPEEVRDKYDLSS 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 364 LRVNISGSAALPTPVKAA----WaalsgGNVLLERYGMTEVGMALSCGLAFADRVDASVGWPLpsvearlvdmdTGDVie 439
Cdd:PRK13391 277 LEVAIHAAAPCPPQVKEQmidwW-----GPIIHEYYAATEGLGFTACDSEEWLAHPGTVGRAM-----------FGDL-- 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 440 pgHELDDHGRE----RVGEIQLRGPTVFrEYWRNPDATAKEFVDDGGdgrgrWFKTGDVAIrrepVGAAGrnshrqpwaq 515
Cdd:PRK13391 339 --HILDDDGAElppgEPGTIWFEGGRPF-EYLNDPAKTAEARHPDGT-----WSTVGDIGY----VDEDG---------- 396
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 516 gpMYFIQGRKTADIIkTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIIL----DPDHAVKwtpLEMRRA 591
Cdd:PRK13391 397 --YLYLTDRAAFMII-SGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPvdgvDPGPALA---AELIAF 470
|
570 580 590
....*....|....*....|....*....|....
gi 573989775 592 LKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLV 625
Cdd:PRK13391 471 CRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLR 504
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
70-624 |
4.72e-27 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 115.52 E-value: 4.72e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 70 VAAHDPTSTAVVHAlsGRRFTYGELLGDV-ARARERLREAVdGKpltGERVAfliensydyvvpeAHSRDVV-MFLAVMA 147
Cdd:PRK07470 16 AARRFPDRIALVWG--DRSWTWREIDARVdALAAALAARGV-RK---GDRIL-------------VHSRNCNqMFESMFA 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 148 A---RAIAVP----LSPPfpapELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVY------FELPKHTGSSHGAK 214
Cdd:PRK07470 77 AfrlGAVWVPtnfrQTPD----EVAYLAEASGARAMICHADFPEHAAAVRAASPDLTHVVaiggarAGLDYEALVARHLG 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 215 EAVSLADEDFSGAGLMLYTSGTTNRPKGVLLPEP----VITAQCRSLLQAweYSPADRLLHVLPLHH---IHGTIN---A 284
Cdd:PRK07470 153 ARVANAAVDHDDPCWFFFTSGTTGRPKAAVLTHGqmafVITNHLADLMPG--TTEQDASLVVAPLSHgagIHQLCQvarG 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 285 VLTPLFAGSsvefmfPFNADAVWkRFAAPFLPTNgtattappisFFTvVPTVYsRLLASHKTLspqmqaatsEATSPANL 364
Cdd:PRK07470 231 AATVLLPSE------RFDPAEVW-ALVERHRVTN----------LFT-VPTIL-KMLVEHPAV---------DRYDHSSL 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 365 R-VNISGSAALPTPVKAAWAALsgGNVLLERYGMTEVGMA---LSCGLAFADRVDA----SVGWPLPSVEARLVDmDTGD 436
Cdd:PRK07470 283 RyVIYAGAPMYRADQKRALAKL--GKVLVQYFGLGEVTGNitvLPPALHDAEDGPDarigTCGFERTGMEVQIQD-DEGR 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 437 VIEPGhelddhgreRVGEIQLRGPTVFREYWRNPDATAKEFVDDggdgrgrWFKTGDVAirrepvgaagrnsHRQpwAQG 516
Cdd:PRK07470 360 ELPPG---------ETGEICVIGPAVFAGYYNNPEANAKAFRDG-------WFRTGDLG-------------HLD--ARG 408
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 517 PMYfIQGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQkVG-AVIILDPDHAvkWTPLEMRRALKSR 595
Cdd:PRK07470 409 FLY-ITGRAS-DMYISGGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGE-VGvAVCVARDGAP--VDEAELLAWLDGK 483
|
570 580
....*....|....*....|....*....
gi 573989775 596 LAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK07470 484 VARYKLPKRFFFWDALPKSGYGKITKKMV 512
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
66-626 |
8.19e-27 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 113.56 E-value: 8.19e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVHAlsGRRFTYGELlgDVARAR--ERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFL 143
Cdd:cd17653 2 AFERIAAAHPDAVAVESL--GGSLTYGEL--DAASNAlaNRLLQLGVVP---GDVVPLLSDRSLEMLV---------AIL 65
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 144 AVMAARAIAVPLSPPFPAPELQYILDQSAASVLVaspkFAAKAREVlatdlqqkpvyfelpkhtgsshgakeavsladed 223
Cdd:cd17653 66 AILKAGAAYVPLDAKLPSARIQAILRTSGATLLL----TTDSPDDL---------------------------------- 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 224 fsgaGLMLYTSGTTNRPKGVLLPEpvitaqcRSLLQAWEYSPAdrLLHVLPlhhihgtinavltplfaGSSVEFMFPFNA 303
Cdd:cd17653 108 ----AYIIFTSGSTGIPKGVMVPH-------RGVLNYVSQPPA--RLDVGP-----------------GSRVAQVLSIAF 157
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 304 DA-VWKRFAApfLPTNGT----------ATTAPPISFFTVVPTVYSrllashkTLSPQmqaatseatSPANLRVNISGSA 372
Cdd:cd17653 158 DAcIGEIFST--LCNGGTlvladpsdpfAHVARTVDALMSTPSILS-------TLSPQ---------DFPNLKTIFLGGE 219
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 373 ALPTPVKAAWaalSGGNVLLERYGMTEVgmalSCGLAFA---DRVDASVGWPLPSVEARLVDMDTGDVIEPghelddhgr 449
Cdd:cd17653 220 AVPPSLLDRW---SPGRRLYNAYGPTEC----TISSTMTellPGQPVTIGKPIPNSTCYILDADLQPVPEG--------- 283
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 450 eRVGEIQLRGPTVFREYWRNPDATAKEFVDDGGDGRGRWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIqGRKTaDI 529
Cdd:cd17653 284 -VVGEICISGVQVARGYLGNPALTASKFVPDPFWPGSRMYRTGDYGRWTE---------------DGGLEFL-GRED-NQ 345
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 530 IKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWgqkVGAVIILDPDHAvkwtplEMRRALKSRLAGYKIPQVMKIVN 609
Cdd:cd17653 346 VKVRGFRINLEEIEEVVLQSQPEVTQAAAIVVNGRL---VAFVTPETVDVD------GLRSELAKHLPSYAVPDRIIALD 416
|
570
....*....|....*..
gi 573989775 610 HIPRNAMGKINKKQLVK 626
Cdd:cd17653 417 SFPLTANGKVDRKALRE 433
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
66-624 |
2.30e-26 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 112.99 E-value: 2.30e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAV---AAHDPTSTAVVHALSGRRFTYGELLGDVARARERLREavDGkPLTGERVAFLIENSYDYVVpeahsrdvvMF 142
Cdd:cd05923 3 VFEMLrraASRAPDACAIADPARGLRLTYSELRARIEAVAARLHA--RG-LRPGQRVAVVLPNSVEAVI---------AL 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 143 LAVMAARAIAVPLSPPFPAPELQYIL--DQSAASVL--VASPKFAAKAREVLATDLQQKPvyfelpkHTGSSHGAKEAVS 218
Cdd:cd05923 71 LALHRLGAVPALINPRLKAAELAELIerGEMTAAVIavDAQVMDAIFQSGVRVLALSDLV-------GLGEPESAGPLIE 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 219 LADEDFSGAGLMLYTSGTTNRPKGVLLPEPviTAQCRSLLQAWE----YSPADRLLHVLPLHHIHGtINAVLTPLFAGSS 294
Cdd:cd05923 144 DPPREPEQPAFVFYTSGTTGLPKGAVIPQR--AAESRVLFMSTQaglrHGRHNVVLGLMPLYHVIG-FFAVLVAALALDG 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 295 ----VEFMFPFNADAVWKRFAapflptngtattappISFFTVVPTVYSRLLAshktlspqmqAATSEATSPANLRVNISG 370
Cdd:cd05923 221 tyvvVEEFDPADALKLIEQER---------------VTSLFATPTHLDALAA----------AAEFAGLKLSSLRHVTFA 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 371 SAALPTPV-KAAWAALSGGNVllERYGMTEVGMALscglaFADRVDA-SVGWPLPSVEARLVDmdtgdVIEPGHELDDHG 448
Cdd:cd05923 276 GATMPDAVlERVNQHLPGEKV--NIYGTTEAMNSL-----YMRDARTgTEMRPGFFSEVRIVR-----IGGSPDEALANG 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 449 RErvGEI--QLRGPTVFREYWRNPDATAKEFVDdggdgrgRWFKTGDVAIrrepVGAAGRnshrqpwaqgpmYFIQGRkT 526
Cdd:cd05923 344 EE--GELivAAAADAAFTGYLNQPEATAKKLQD-------GWYRTGDVGY----VDPSGD------------VRILGR-V 397
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 527 ADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVKWTPLEMRRAlkSRLAGYKIPQVMK 606
Cdd:cd05923 398 DDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPREGTLSADELDQFCRA--SELADFKRPRRYF 475
|
570
....*....|....*...
gi 573989775 607 IVNHIPRNAMGKINKKQL 624
Cdd:cd05923 476 FLDELPKNAMNKVLRRQL 493
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
66-626 |
5.02e-26 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 114.67 E-value: 5.02e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVhaLSGRRFTYGELLGDVARARERLREAVDGkplTGERVAFLIENSYDYVVPeahsrdvvmFLAV 145
Cdd:PRK12316 4556 LVAERARMTPDAVAVV--FDEEKLTYAELNRRANRLAHALIARGVG---PEVLVGIAMERSAEMMVG---------LLAV 4621
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAK---AREVLATDLQQKPVYFELPKHTgsshgakEAVSLADE 222
Cdd:PRK12316 4622 LKAGGAYVPLDPEYPRERLAYMMEDSGAALLLTQSHLLQRlpiPDGLASLALDRDEDWEGFPAHD-------PAVRLHPD 4694
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 223 DFSgagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHhIHGTINAVLTPLFAGSSVEFmfpfN 302
Cdd:PRK12316 4695 NLA---YVIYTSGSTGRPKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSFS-FDGSHEGLYHPLINGASVVI----R 4766
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 303 ADAVWKRFAAPFLPTNGTATTAppisfftVVPTVYSRLLASHktlspqmqaaTSEATSPANLRVNISGSAAL-PTPVKAA 381
Cdd:PRK12316 4767 DDSLWDPERLYAEIHEHRVTVL-------VFPPVYLQQLAEH----------AERDGEPPSLRVYCFGGEAVaQASYDLA 4829
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 382 WAALSGGNvLLERYGMTEVGMALSC----GLAFADRVDASVGWPLPSVEARLVDmDTGDVIEPGhelddhgreRVGEIQL 457
Cdd:PRK12316 4830 WRALKPVY-LFNGYGPTETTVTVLLwkarDGDACGAAYMPIGTPLGNRSGYVLD-GQLNPLPVG---------VAGELYL 4898
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 458 RGPTVFREYWRNPDATAKEFVDDG-GDGRGRWFKTGDVAIRREP--VGAAGRNSHRqpwaqgpmyfiqgrktadiIKTGG 534
Cdd:PRK12316 4899 GGEGVARGYLERPALTAERFVPDPfGAPGGRLYRTGDLARYRADgvIDYLGRVDHQ-------------------VKIRG 4959
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 535 EKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDP---DHAVKWTPL--EMRRALKSRLAGYKIPQVMKIVN 609
Cdd:PRK12316 4960 FRIELGEIEARLREHPAVREAVVIAQEGAVGKQLVGYVVPQDPalaDADEAQAELrdELKAALRERLPEYMVPAHLVFLA 5039
|
570
....*....|....*..
gi 573989775 610 HIPRNAMGKINKKQLVK 626
Cdd:PRK12316 5040 RMPLTPNGKLDRKALPQ 5056
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
75-624 |
5.82e-26 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 111.59 E-value: 5.82e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGkplTGERVAFLIENSydyvvPEAhsrdVVMFLAVMAARAIAVP 154
Cdd:cd12114 1 PDATAVICG--DGTLTYGELAERARRVAGALKAAGVR---PGDLVAVTLPKG-----PEQ----VVAVLGILAAGAAYVP 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 155 LSPPFPAPELQYILDQSAAS-VLVASPkfaakarevlatDLQQKPVYFELPKHTGSSHGAKEAVSLADEDFSGAGLMLYT 233
Cdd:cd12114 67 VDIDQPAARREAILADAGARlVLTDGP------------DAQLDVAVFDVLILDLDALAAPAPPPPVDVAPDDLAYVIFT 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 234 SGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHH------IHGtinavltPLFAGSSVefMFPFNAD--- 304
Cdd:cd12114 135 SGSTGTPKGVMISHRAALNTILDINRRFAVGPDDRVLALSSLSFdlsvydIFG-------ALSAGATL--VLPDEARrrd 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 305 -AVWKRFAAPFlptngtattapPISFFTVVPTVYSRLLAshktlspqmqAATSEATSPANLR-VNISG---SAALPTPVK 379
Cdd:cd12114 206 pAHWAELIERH-----------GVTLWNSVPALLEMLLD----------VLEAAQALLPSLRlVLLSGdwiPLDLPARLR 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 380 AAWAA---LSGGnvlleryGMTEVGM-ALSCGLAFADRVDASV--GWPLPSVEARLVDmdtgdviepgheldDHGRER-- 451
Cdd:cd12114 265 ALAPDarlISLG-------GATEASIwSIYHPIDEVPPDWRSIpyGRPLANQRYRVLD--------------PRGRDCpd 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 452 --VGEIQLRGPTVFREYWRNPDATAKEFVDDGGdgRGRWFKTGDVairrepvgaaGRNshrqpWAQGPMYFIqGRKtaDI 529
Cdd:cd12114 324 wvPGELWIGGRGVALGYLGDPELTAARFVTHPD--GERLYRTGDL----------GRY-----RPDGTLEFL-GRR--DG 383
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 530 -IKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNwGQKVGAVIILDPDHAVKwTPLEMRRALKSRLAGYKIPQVMKIV 608
Cdd:cd12114 384 qVKVRGYRIELGEIEAALQAHPGVARAVVVVLGDPG-GKRLAAFVVPDNDGTPI-APDALRAFLAQTLPAYMIPSRVIAL 461
|
570
....*....|....*.
gi 573989775 609 NHIPRNAMGKINKKQL 624
Cdd:cd12114 462 EALPLTANGKVDRAAL 477
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
143-495 |
9.69e-26 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 111.54 E-value: 9.69e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 143 LAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYFELPKhtgsshgaKEAVSLade 222
Cdd:cd05927 50 LACYAYSLVTVPLYDTLGPEAIEYILNHAEISIVFCDAGVKVYSLEEFEKLGKKNKVPPPPPK--------PEDLAT--- 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 223 dfsgaglMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWE----YSPADRLLHVLPLHHIHGTINAVLTpLFAGSSVEFm 298
Cdd:cd05927 119 -------ICYTSGTTGNPKGVMLTHGNIVSNVAGVFKILEilnkINPTDVYISYLPLAHIFERVVEALF-LYHGAKIGF- 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 299 fpFNADavwkrfaaPFLPTNGTATTAPpiSFFTVVPTVYSRLLA---------------------SHKTLspQMQAATSE 357
Cdd:cd05927 190 --YSGD--------IRLLLDDIKALKP--TVFPGVPRVLNRIYDkifnkvqakgplkrklfnfalNYKLA--ELRSGVVR 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 358 ATS--------------PANLRVNISGSAALPTPVKAAWAALSGGNVLlERYGMTEvgmalSCGLAFA----DRVDASVG 419
Cdd:cd05927 256 ASPfwdklvfnkikqalGGNVRLMLTGSAPLSPEVLEFLRVALGCPVL-EGYGQTE-----CTAGATLtlpgDTSVGHVG 329
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 573989775 420 WPLPSVEARLVDmdtgdVIEPG-HELDDHGRervGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVA 495
Cdd:cd05927 330 GPLPCAEVKLVD-----VPEMNyDAKDPNPR---GEVCIRGPNVFSGYYKDPEKTAEALDEDG------WLHTGDIG 392
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
89-621 |
2.92e-25 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 109.14 E-value: 2.92e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 89 FTYGELLGDVARARERLREAVDGkplTGERVAFLIENSydyvvPEAhsrdVVMFLAVMAARAIAVPLSPPFPAPELQYIL 168
Cdd:cd05973 1 LTFGELRALSARFANALQELGVG---PGDVVAGLLPRT-----PEL----VVTILGIWRLGAVYQPLFTAFGPKAIEHRL 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 169 DQSAASVLVASpkfaAKAREVLATDLQqkpvyfelpkhtgsshgakeavsladedfsgagLMLYTSGTTNRPKGVLLPEP 248
Cdd:cd05973 69 RTSGARLVVTD----AANRHKLDSDPF---------------------------------VMMFTSGTTGLPKGVPVPLR 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 249 VITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAG-SSVEFMFPFNADAVWK---RFAapflptngtatta 324
Cdd:cd05973 112 ALAAFGAYLRDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALGhPTILLEGGFSVESTWRvieRLG------------- 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 325 ppISFFTVVPTVYsRLLashktlspqMQAATSEATSPaNLRVNISGSAALP-TPVKAAWAALSGGNVLLERYGMTEVGMA 403
Cdd:cd05973 179 --VTNLAGSPTAY-RLL---------MAAGAEVPARP-KGRLRRVSSAGEPlTPEVIRWFDAALGVPIHDHYGQTELGMV 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 404 LSCGLAFADRVDA-SVGWPLPSVEARLVDMDTGDVI--EPGhelddhgreRVGEIQLRGPTV-FREYWRNPDATAKefvd 479
Cdd:cd05973 246 LANHHALEHPVHAgSAGRAMPGWRVAVLDDDGDELGpgEPG---------RLAIDIANSPLMwFRGYQLPDTPAID---- 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 480 dggdgrGRWFKTGDvAIRREPvgaagrnshrqpwaQGPMYFIqGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVA 559
Cdd:cd05973 313 ------GGYYLTGD-TVEFDP--------------DGSFSFI-GRAD-DVITMSGYRIGPFDVESALIEHPAVAEAAVIG 369
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 573989775 560 VPSGNWGQKVGAVIILDPDHavKWTPL---EMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINK 621
Cdd:cd05973 370 VPDPERTEVVKAFVVLRGGH--EGTPAladELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQR 432
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
86-624 |
4.11e-25 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 109.34 E-value: 4.11e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 86 GRRFTYGELL-GDVARAReRLREAVDGkpltGERVAFLIENSydyvvpeahSRDVVMFLAVMAARAIAVPLSPPFPAPEL 164
Cdd:cd05909 5 GTSLTYRKLLtGAIALAR-KLAKMTKE----GENVGVMLPPS---------AGGALANFALALSGKVPVMLNYTAGLREL 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 165 QYILDQSAASVLVASPKFAAKAREVLATDL--QQKPVYFElpkhtgsshGAKEAVSLADEDFSGAGLM------------ 230
Cdd:cd05909 71 RACIKLAGIKTVLTSKQFIEKLKLHHLFDVeyDARIVYLE---------DLRAKISKADKCKAFLAGKfppkwllrifgv 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 231 -----------LYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVeFMF 299
Cdd:cd05909 142 apvqpddpaviLFTSGSEGLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKV-VFH 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 300 PFnadavwkrfaapflPTNGTattappisffTVVPTVYSR----LLASHKTLSPQMQAATSEATSpaNLRVNISGSAALP 375
Cdd:cd05909 221 PN--------------PLDYK----------KIPELIYDKkatiLLGTPTFLRGYARAAHPEDFS--SLRLVVAGAEKLK 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 376 TPVKAAWAALSgGNVLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDmdtgdviEPGHELDDHGRErvGEI 455
Cdd:cd05909 275 DTLRQEFQEKF-GIRILEGYGTTECSPVISVNTPQSPNKEGTVGRPLPGMEVKIVS-------VETHEEVPIGEG--GLL 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 456 QLRGPTVFREYWRNPDATAKEFvddgGDGrgrWFKTGDvairrepVGAAGRNSHrqpwaqgpmYFIQGRkTADIIKTGGE 535
Cdd:cd05909 345 LVRGPNVMLGYLNEPELTSFAF----GDG---WYDTGD-------IGKIDGEGF---------LTITGR-LSRFAKIAGE 400
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 536 KVSALEVERELLS-LPQVAEAAVVAVPSGNWGQKVgaVIILDPDHAvkwTPLEMRRALKSrlagYKIPQVMKIVNH---- 610
Cdd:cd05909 401 MVSLEAIEDILSEiLPEDNEVAVVSVPDGRKGEKI--VLLTTTTDT---DPSSLNDILKN----AGISNLAKPSYIhqve 471
|
570
....*....|....*
gi 573989775 611 -IPRNAMGKINKKQL 624
Cdd:cd05909 472 eIPLLGTGKPDYVTL 486
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
89-628 |
7.54e-25 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 107.97 E-value: 7.54e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 89 FTYGELLGDVARARERLREAVDGKpltGERVAFLIENSydyvvPEAHsrdvVMFLAVMAARAIAVPLSPPFPAPELQYIL 168
Cdd:cd05969 1 YTFAQLKVLSARFANVLKSLGVGK---GDRVFVLSPRS-----PELY----FSMLGIGKIGAVICPLFSAFGPEAIRDRL 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 169 DQSAASVLVASPKFAAKarevlaTDLqqkpvyfelpkhtgsshgakeavsladEDFSgagLMLYTSGTTNRPKGVLLPEP 248
Cdd:cd05969 69 ENSEAKVLITTEELYER------TDP---------------------------EDPT---LLHYTSGTTGTPKGVLHVHD 112
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 249 VITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAG-SSVEFMFPFNADAvWKrfaapflptngTATTAPPI 327
Cdd:cd05969 113 AMIFYYFTGKYVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNGvTNVVYEGRFDAES-WY-----------GIIERVKV 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 328 SFFTVVPTVYSRLLASHKTLspqmqAATSEATSpanLRVNISGSAALpTPVKAAWAALSGGNVLLERYGMTEVGMALSCG 407
Cdd:cd05969 181 TVWYTAPTAIRMLMKEGDEL-----ARKYDLSS---LRFIHSVGEPL-NPEAIRWGMEVFGVPIHDTWWQTETGSIMIAN 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 408 LAFADRVDASVGWPLPSVEARLVDMDtGDVIEPGHelddhgrerVGEIQLRG--PTVFREYWRNPDATAKEFVDDggdgr 485
Cdd:cd05969 252 YPCMPIKPGSMGKPLPGVKAAVVDEN-GNELPPGT---------KGILALKPgwPSMFRGIWNDEERYKNSFIDG----- 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 486 grWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFiQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNW 565
Cdd:cd05969 317 --WYLTGDLAYRDE---------------DGYFWF-VGR-ADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLR 377
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 573989775 566 GQKVGAVIILDPDH-AVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKqLVKAI 628
Cdd:cd05969 378 GEIIKAFISLKEGFePSDELKEEIINFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRR-VLKAK 440
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
116-624 |
3.63e-24 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 106.88 E-value: 3.63e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 116 GERVAFLIENSYDYVVPEahsrdvvmfLAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLA-TDL 194
Cdd:PRK08751 76 GDRVALMMPNCLQYPIAT---------FGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQVIAdTPV 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 195 QQ----------------------KPVYFELPKHTGS---------SHGAKEAVSLADEDFSGAGLMLYTSGTTNRPKGV 243
Cdd:PRK08751 147 KQvittglgdmlgfpkaalvnfvvKYVKKLVPEYRINgairfrealALGRKHSMPTLQIEPDDIAFLQYTGGTTGVAKGA 226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 244 LLPEPVITAqcrSLLQAWEY--------SPADRLLHVLPLHHIHG-TINAVLTPLFAGSSVEFMFPFNADAVWKRFaapf 314
Cdd:PRK08751 227 MLTHRNLVA---NMQQAHQWlagtgkleEGCEVVITALPLYHIFAlTANGLVFMKIGGCNHLISNPRDMPGFVKEL---- 299
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 315 lptNGTATTAppisfFTVVPTVYSRLLAShktlsPQMqaatsEATSPANLRVNISGSAALPTPVKAAWAALSGgNVLLER 394
Cdd:PRK08751 300 ---KKTRFTA-----FTGVNTLFNGLLNT-----PGF-----DQIDFSSLKMTLGGGMAVQRSVAERWKQVTG-LTLVEA 360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 395 YGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDmDTGDVIEPGHelddhgrerVGEIQLRGPTVFREYWRNPDATA 474
Cdd:PRK08751 361 YGLTETSPAACINPLTLKEYNGSIGLPIPSTDACIKD-DAGTVLAIGE---------IGELCIKGPQVMKGYWKRPEETA 430
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 475 KEFVDDGgdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIQGRKtaDIIKTGGEKVSALEVERELLSLPQVAE 554
Cdd:PRK08751 431 KVMDADG------WLHTGDIARMDE---------------QGFVYIVDRKK--DMILVSGFNVYPNEIEDVIAMMPGVLE 487
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 573989775 555 AAVVAVPSGNWGQKVGAVII-LDPDhavkWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK08751 488 VAAVGVPDEKSGEIVKVVIVkKDPA----LTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRREL 554
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
77-624 |
1.81e-23 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 104.88 E-value: 1.81e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 77 STAVVHALSGRRFTYGELLGDVARARERLreaVDGKPLTGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLS 156
Cdd:PLN02860 21 GNAVVTISGNRRRTGHEFVDGVLSLAAGL---LRLGLRNGDVVAIAALNSDLYLE---------WLLAVACAGGIVAPLN 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 157 PPFPAPELQ----------YILDQSAAS----------------VLVASP--KFAAKAREVLATDlqqkpvyfELPKHTG 208
Cdd:PLN02860 89 YRWSFEEAKsamllvrpvmLVTDETCSSwyeelqndrlpslmwqVFLESPssSVFIFLNSFLTTE--------MLKQRAL 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 209 SShgakeavSLADEDFS--GAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIhGTINAVL 286
Cdd:PLN02860 161 GT-------TELDYAWApdDAVLICFTSGTTGRPKGVTISHSALIVQSLAKIAIVGYGEDDVYLHTAPLCHI-GGLSSAL 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 287 TPLFAGSSVEFMFPFNADAVWKrfaapflptngtATTAPPISFFTVVPTVYSRLLashktlSPQMQAATSEATSpaNLRV 366
Cdd:PLN02860 233 AMLMVGACHVLLPKFDAKAALQ------------AIKQHNVTSMITVPAMMADLI------SLTRKSMTWKVFP--SVRK 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 367 NISGSAALPTPVKAAWAALSGGNVLLERYGMTEVGMALS-------------CGLAFADRVDAS---------VGWPLPS 424
Cdd:PLN02860 293 ILNGGGSLSSRLLPDAKKLFPNAKLFSAYGMTEACSSLTfmtlhdptlespkQTLQTVNQTKSSsvhqpqgvcVGKPAPH 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 425 VEARlVDMDTGDviepghelddhgreRVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaA 504
Cdd:PLN02860 373 VELK-IGLDESS--------------RVGRILTRGPHVMLGYWGQNSETASVLSNDG------WLDTGDIGWIDK----A 427
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 505 GRnshrqPWAQGpmyfiqgrKTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILdpDHAVKWT 584
Cdd:PLN02860 428 GN-----LWLIG--------RSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVPDSRLTEMVVACVRL--RDGWIWS 492
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*..
gi 573989775 585 PLEMRRALKSR---------------LAGYKIPQVMkIVNH--IPRNAMGKINKKQL 624
Cdd:PLN02860 493 DNEKENAKKNLtlssetlrhhcreknLSRFKIPKLF-VQWRkpFPLTTTGKIRRDEV 548
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
66-627 |
2.52e-23 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 106.01 E-value: 2.52e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVHAlsGRRFTYGELlgdVARArERLREAVDGKPLTGE-RVAFLIENSYDYVVPeahsrdvvmFLA 144
Cdd:PRK12467 1579 LIEDQAAATPEAVALVFG--EQELTYGEL---NRRA-NRLAHRLIALGVGPEvLVGIAVERSLEMVVG---------LLA 1643
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 145 VMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKarevLATDLQQKPVYFELPKHTGSSHG-AKEAVSLADED 223
Cdd:PRK12467 1644 ILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQSHLQAR----LPLPDGLRSLVLDQEDDWLEGYSdSNPAVNLAPQN 1719
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 224 FSgagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHhihgtinavltplFAGSSVEFMFPF-- 301
Cdd:PRK12467 1720 LA---YVIYTSGSTGRPKGAGNRHGALVNRLCATQEAYQLSAADVVLQFTSFA-------------FDVSVWELFWPLin 1783
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 302 NADAVWKRFAAPFLPTNGTAT-TAPPISFFTVVPTVYSRLLashktlspQMQAATSEATSpanLRVNISGSAALPTPVKA 380
Cdd:PRK12467 1784 GARLVIAPPGAHRDPEQLIQLiERQQVTTLHFVPSMLQQLL--------QMDEQVEHPLS---LRRVVCGGEALEVEALR 1852
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 381 AWAALSGGNVLLERYGMTEVgmALSCGLAFADRVD------ASVGWPLPSVEARLVDmdtgdviepgHELDDHGRERVGE 454
Cdd:PRK12467 1853 PWLERLPDTGLFNLYGPTET--AVDVTHWTCRRKDlegrdsVPIGQPIANLSTYILD----------ASLNPVPIGVAGE 1920
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 455 IQLRGPTVFREYWRNPDATAKEFVDDG-GDGRGRWFKTGDVAiRREPVGA---AGRNSHRqpwaqgpmyfiqgrktadiI 530
Cdd:PRK12467 1921 LYLGGVGLARGYLNRPALTAERFVADPfGTVGSRLYRTGDLA-RYRADGVieyLGRIDHQ-------------------V 1980
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 531 KTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDP---DHAVKWTPL--EMRRALKSRLAGYKIPQVM 605
Cdd:PRK12467 1981 KIRGFRIELGEIEARLREQGGVREAVVIAQDGANGKQLVAYVVPTDPglvDDDEAQVALraILKNHLKASLPEYMVPAHL 2060
|
570 580
....*....|....*....|..
gi 573989775 606 KIVNHIPRNAMGKINKKQLVKA 627
Cdd:PRK12467 2061 VFLARMPLTPNGKLDRKALPAP 2082
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
232-624 |
2.60e-23 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 101.79 E-value: 2.60e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 232 YTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFN------ADA 305
Cdd:cd05944 9 HTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGAHVVLAGPAGyrnpglFDN 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 306 VWKrFAAPFLPTngtattappisFFTVVPTVYSRLlashktlspqMQAATSEATSpaNLRVNISGSAALPTPVKAAWAAL 385
Cdd:cd05944 89 FWK-LVERYRIT-----------SLSTVPTVYAAL----------LQVPVNADIS--SLRFAMSGAAPLPVELRARFEDA 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 386 SGGNVlLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDMDtGDviepGHELDDHGRERVGEIQLRGPTVFRE 465
Cdd:cd05944 145 TGLPV-VEGYGLTEATCLVAVNPPDGPKRPGSVGLRLPYARVRIKVLD-GV----GRLLRDCAPDEVGEICVAGPGVFGG 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 466 YWRNPDatakefvDDGGDGRGRWFKTGDVAirrePVGAAGrnshrqpwaqgpMYFIQGRkTADIIKTGGEKVSALEVERE 545
Cdd:cd05944 219 YLYTEG-------NKNAFVADGWLNTGDLG----RLDADG------------YLFITGR-AKDLIIRGGHNIDPALIEEA 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 546 LLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVKWTPLE--MRRALKSRLAgykIPQVMKIVNHIPRNAMGKINKKQ 623
Cdd:cd05944 275 LLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAVVEEEELLawARDHVPERAA---VPKHIEVLEELPVTAVGKVFKPA 351
|
.
gi 573989775 624 L 624
Cdd:cd05944 352 L 352
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
66-624 |
4.01e-23 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 103.01 E-value: 4.01e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVvHALSGRrFTYGELlgDVARAR--ERLREAVDGkplTGERVAFLIENSydyvvPEAhsrdVVMFL 143
Cdd:cd05918 4 LIEERARSQPDAPAV-CAWDGS-LTYAEL--DRLSSRlaHHLRSLGVG---PGVFVPLCFEKS-----KWA----VVAML 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 144 AVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASpkfaakarevlatdlqqkpvyfelpkhtgsshgakeavsladeD 223
Cdd:cd05918 68 AVLKAGGAFVPLDPSHPLQRLQEILQDTGAKVVLTS-------------------------------------------S 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 224 FSGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHvLPLHHIHGTINAVLTPLFAGSSV-------- 295
Cdd:cd05918 105 PSDAAYVIFTSGSTGKPKGVVIEHRALSTSALAHGRALGLTSESRVLQ-FASYTFDVSILEIFTTLAAGGCLcipseedr 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 296 -----EFMfpfnadavwKRFAApflptngtaTTAppisFFTvvPTVySRLLashktlSPQmqaatseatSPANLRVNISG 370
Cdd:cd05918 184 lndlaGFI---------NRLRV---------TWA----FLT--PSV-ARLL------DPE---------DVPSLRTLVLG 223
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 371 SAALPTPVKAAWAalsGGNVLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVeARLVDmdtgdviepgheLDDHGRE 450
Cdd:cd05918 224 GEALTQSDVDTWA---DRVRLINAYGPAECTIAATVSPVVPSTDPRNIGRPLGAT-CWVVD------------PDNHDRL 287
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 451 R----VGEIQLRGPTVFREYWRNPDATAKEFVDD-------GGDGRGRWFKTGDVAiRREPvgaagrnshrqpwaQGPMY 519
Cdd:cd05918 288 VpigaVGELLIEGPILARGYLNDPEKTAAAFIEDpawlkqeGSGRGRRLYRTGDLV-RYNP--------------DGSLE 352
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 520 FIqGRKTADIiKTGGEKVSALEVE---RELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDH-------------AVKW 583
Cdd:cd05918 353 YV-GRKDTQV-KIRGQRVELGEIEhhlRQSLPGAKEVVVEVVKPKDGSSSPQLVAFVVLDGSSsgsgdgdslflepSDEF 430
|
570 580 590 600
....*....|....*....|....*....|....*....|...
gi 573989775 584 TPL--EMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd05918 431 RALvaELRSKLRQRLPSYMVPSVFLPLSHLPLTASGKIDRRAL 473
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
66-495 |
1.10e-22 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 102.37 E-value: 1.10e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAvAAHDPTSTAVVHALSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSydyvvPEAhsrdVVMFLAV 145
Cdd:PLN02246 29 CFER-LSEFSDRPCLIDGATGRVYTYADVELLSRRVAAGLHKLGIRQ---GDVVMLLLPNC-----PEF----VLAFLGA 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKARE--------VLATDlQQKPVYFELPKHTGSSHGAKEAV 217
Cdd:PLN02246 96 SRRGAVTTTANPFYTPAEIAKQAKASGAKLIITQSCYVDKLKGlaeddgvtVVTID-DPPEGCLHFSELTQADENELPEV 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 218 SLADEDFSGaglMLYTSGTTNRPKGVLLP-EPVITaqcrSLLQAWE-------YSPADRLLHVLPLHHIHgTINAV-LTP 288
Cdd:PLN02246 175 EISPDDVVA---LPYSSGTTGLPKGVMLThKGLVT----SVAQQVDgenpnlyFHSDDVILCVLPMFHIY-SLNSVlLCG 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 289 LFAGSSVEFMFPFNADAV------WKRFAAPFlptngtattAPPIsfftvvptvysrLLASHKtlSPQMqaatsEATSPA 362
Cdd:PLN02246 247 LRVGAAILIMPKFEIGALleliqrHKVTIAPF---------VPPI------------VLAIAK--SPVV-----EKYDLS 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 363 NLRVNISGSAALPTPVKAAWAALSGGNVLLERYGMTEVGMALSCGLAFADRV----DASVGWPLPSVEARLVDMDTGDVI 438
Cdd:PLN02246 299 SIRMVLSGAAPLGKELEDAFRAKLPNAVLGQGYGMTEAGPVLAMCLAFAKEPfpvkSGSCGTVVRNAELKIVDPETGASL 378
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*..
gi 573989775 439 epghelddhGRERVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVA 495
Cdd:PLN02246 379 ---------PRNQPGEICIRGPQIMKGYLNDPEATANTIDKDG------WLHTGDIG 420
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
228-618 |
2.87e-22 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 98.99 E-value: 2.87e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 228 GLMLYTSGTTNRPKGVL---------------LPEPVITAQcRSLLQAWEYSPADRLLHVLPLhhIHGT-INAVLTPLFA 291
Cdd:cd05924 6 LYILYTGGTTGMPKGVMwrqedifrmlmggadFGTGEFTPS-EDAHKAAAAAAGTVMFPAPPL--MHGTgSWTAFGGLLG 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 292 GSSVEFMFP-FNADAVWK---RFAAPFLPTNGTATTAPPISFFTvvptvysrllashktlspqmQAATSEATSpanLRVN 367
Cdd:cd05924 83 GQTVVLPDDrFDPEEVWRtieKHKVTSMTIVGDAMARPLIDALR--------------------DAGPYDLSS---LFAI 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 368 ISGSAALPTPVKAAWAALSGGNVLLERYGMTEVGMAlscGLAFADRVDASVG-WPLPSVEARLVDMDTGdVIEPGHELdd 446
Cdd:cd05924 140 SSGGALLSPEVKQGLLELVPNITLVDAFGSSETGFT---GSGHSAGSGPETGpFTRANPDTVVLDDDGR-VVPPGSGG-- 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 447 hgrerVGEIQLRGpTVFREYWRNPDATAKEFVDDGGDgrgRWFKTGDVAiRREPVGAA---GRNSHrqpwaqgpmyfiqg 523
Cdd:cd05924 214 -----VGWIARRG-HIPLGYYGDEAKTAETFPEVDGV---RYAVPGDRA-TVEADGTVtllGRGSV-------------- 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 524 rktadIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQ 603
Cdd:cd05924 270 -----CINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQEVVAVVQLREGAGV--DLEELREHCRTRIARYKLPK 342
|
410
....*....|....*
gi 573989775 604 VMKIVNHIPRNAMGK 618
Cdd:cd05924 343 QVVFVDEIERSPAGK 357
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
89-550 |
6.87e-22 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 99.47 E-value: 6.87e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 89 FTYGELLGDVARARERLReAVDGKPltGERVAFLIENSYDYVVPEahsrdvvmfLAVMAARAIAVPLSPPFPAPELQYIL 168
Cdd:cd05932 7 FTWGEVADKARRLAAALR-ALGLEP--GSKIALISKNCAEWFITD---------LAIWMAGHISVPLYPTLNPDTIRYVL 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 169 DQSAASVLVASPKFAAKARE-VLATDLQQKPVYF--ELPKHTGSSHGAKEAVSLADEDFSGA---GLMLYTSGTTNRPKG 242
Cdd:cd05932 75 EHSESKALFVGKLDDWKAMApGVPEGLISISLPPpsAANCQYQWDDLIAQHPPLEERPTRFPeqlATLIYTSGTTGQPKG 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 243 VLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEF---MFPFNADAVWKR----FAAPFL 315
Cdd:cd05932 155 VMLTFGSFAWAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAFaesLDTFVEDVQRARptlfFSVPRL 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 316 PT---NGTATTAPP--ISFFTVVPTVYSrlLASHKTLspqmqaatsEATSPANLRVNISGSAALPtPVKAAWAALSGGNV 390
Cdd:cd05932 235 WTkfqQGVQDKIPQqkLNLLLKIPVVNS--LVKRKVL---------KGLGLDQCRLAGCGSAPVP-PALLEWYRSLGLNI 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 391 lLERYGMTEvGMALSCGLAFADRVDASVGWPLPSVEARLVDMdtgdviepghelddhgrervGEIQLRGPTVFREYWRNP 470
Cdd:cd05932 303 -LEAYGMTE-NFAYSHLNYPGRDKIGTVGNAGPGVEVRISED--------------------GEILVRSPALMMGYYKDP 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 471 DATAKEFVDDGgdgrgrWFKTGDVAirrePVGAAGRNShrqpwaqgpmyfIQGRKTaDIIKTG-GEKVSALEVERELLSL 549
Cdd:cd05932 361 EATAEAFTADG------FLRTGDKG----ELDADGNLT------------ITGRVK-DIFKTSkGKYVAPAPIENKLAEH 417
|
.
gi 573989775 550 P 550
Cdd:cd05932 418 D 418
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
75-624 |
8.60e-22 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 99.52 E-value: 8.60e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHALSGRRFTYGELLgdvaRARERLREAVDGKPL-TGERVAFLIENSYDYvvpeahsrdvvmFLAVMAARAIAV 153
Cdd:cd17642 31 PGTIAFTDAHTGVNYSYAEYL----EMSVRLAEALKKYGLkQNDRIAVCSENSLQF------------FLPVIAGLFIGV 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 154 ---PLSPPFPAPELQYILDQSAASVLVASPKFAAKAREV--------------LATDLQQK-----------PVYFELPK 205
Cdd:cd17642 95 gvaPTNDIYNERELDHSLNISKPTIVFCSKKGLQKVLNVqkklkiiktiiildSKEDYKGYqclytfitqnlPPGFNEYD 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 206 HTGSSHGAKEAVSLadedfsgaglMLYTSGTTNRPKGVLLPEPVITAQ---CRSLLQAWEYSPADRLLHVLPLHHIHGTI 282
Cdd:cd17642 175 FKPPSFDRDEQVAL----------IMNSSGSTGLPKGVQLTHKNIVARfshARDPIFGNQIIPDTAILTVIPFHHGFGMF 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 283 NaVLTPLFAGSSVEFMFPFNADAvwkrfaapFLptngTATTAPPISFFTVVPTVYSrLLASHKTLspqmqaatsEATSPA 362
Cdd:cd17642 245 T-TLGYLICGFRVVLMYKFEEEL--------FL----RSLQDYKVQSALLVPTLFA-FFAKSTLV---------DKYDLS 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 363 NLRVNISGSAALPTPVKAAWAALSGGNVLLERYGMTEVGMALscgLAFADRVD--ASVGWPLPSVEARLVDMDTGDVIep 440
Cdd:cd17642 302 NLHEIASGGAPLSKEVGEAVAKRFKLPGIRQGYGLTETTSAI---LITPEGDDkpGAVGKVVPFFYAKVVDLDTGKTL-- 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 441 ghelddhGRERVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIrrepvgaagrnshrqpWAQGPMYF 520
Cdd:cd17642 377 -------GPNERGELCVKGPMIMKGYVNNPEATKALIDKDG------WLHSGDIAY----------------YDEDGHFF 427
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 521 IQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDpdHAVKWTPLEMRRALKSRLAGYK 600
Cdd:cd17642 428 IVDR-LKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVVVLE--AGKTMTEKEVMDYVASQVSTAK 504
|
570 580
....*....|....*....|....*
gi 573989775 601 -IPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd17642 505 rLRGGVKFVDEVPKGLTGKIDRRKI 529
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
222-625 |
1.83e-21 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 97.83 E-value: 1.83e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 222 EDFSGAGLMLYTSGTTNRPKGVL--LPEPVITAqcrSLLQAW----EYSPADRLLHVLPLHHIHGTInAVLTPLFAGSSV 295
Cdd:cd05929 122 EDEAAGWKMLYSGGTTGRPKGIKrgLPGGPPDN---DTLMAAalgfGPGADSVYLSPAPLYHAAPFR-WSMTALFMGGTL 197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 296 EFMFPFNADAVWKrfaapflptngtATTAPPISFFTVVPTVYSRLLAShktlsPQMQAATSEATSpanLRVNISGSAALP 375
Cdd:cd05929 198 VLMEKFDPEEFLR------------LIERYRVTFAQFVPTMFVRLLKL-----PEAVRNAYDLSS---LKRVIHAAAPCP 257
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 376 TPVKAAWAALsGGNVLLERYGMTEvgmalSCGLAFADRVD-----ASVGWPlpsVEARLvdmdtgdviepgHELDDHGRE 450
Cdd:cd05929 258 PWVKEQWIDW-GGPIIWEYYGGTE-----GQGLTIINGEEwlthpGSVGRA---VLGKV------------HILDEDGNE 316
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 451 ----RVGEIQLRGPTVFrEYWRNPDATAKEFVDDGgdgrgrWFKTGDVairrepvgaaGRNShrqpwAQGPMYFiQGRKT 526
Cdd:cd05929 317 vppgEIGEVYFANGPGF-EYTNDPEKTAAARNEGG------WSTLGDV----------GYLD-----EDGYLYL-TDRRS 373
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 527 aDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDhAVKWTPL--EMRRALKSRLAGYKIPQV 604
Cdd:cd05929 374 -DMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVQPAPG-ADAGTALaeELIAFLRDRLSRYKCPRS 451
|
410 420
....*....|....*....|.
gi 573989775 605 MKIVNHIPRNAMGKINKKQLV 625
Cdd:cd05929 452 IEFVAELPRDDTGKLYRRLLR 472
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
86-624 |
3.24e-21 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 97.78 E-value: 3.24e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 86 GRRFTYGELlGDVARArerLREAVDGKPLT-GERVAFLIENSYDYvvPeahsrdvVMFLAVMAARAIAVPLSPPFPAPEL 164
Cdd:PRK07059 46 GKAITYGEL-DELSRA---LAAWLQSRGLAkGARVAIMMPNVLQY--P-------VAIAAVLRAGYVVVNVNPLYTPREL 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 165 QYILDQSAASVLVASPKFAAKAREVLA-TDLQQKPV--------------------------YFELPKH----TGSSHGA 213
Cdd:PRK07059 113 EHQLKDSGAEAIVVLENFATTVQQVLAkTAVKHVVVasmgdllgfkghivnfvvrrvkkmvpAWSLPGHvrfnDALAEGA 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 214 KEA---VSLADEDfsgAGLMLYTSGTTNRPKGVLLPEPVITAqcrSLLQ--AWEYSPADRLLHV--------LPLHHIHG 280
Cdd:PRK07059 193 RQTfkpVKLGPDD---VAFLQYTGGTTGVSKGATLLHRNIVA---NVLQmeAWLQPAFEKKPRPdqlnfvcaLPLYHIFA 266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 281 -TINAVLTPLFAGSSVEFMFPFNADAVWKRFAAPflptngtattapPISFFTVVPTVYSRLlashktlspqMQAATSEAT 359
Cdd:PRK07059 267 lTVCGLLGMRTGGRNILIPNPRDIPGFIKELKKY------------QVHIFPAVNTLYNAL----------LNNPDFDKL 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 360 SPANLRVNISGSAALPTPVKAAWAALSGGNVLlERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDmDTGDVIE 439
Cdd:PRK07059 325 DFSKLIVANGGGMAVQRPVAERWLEMTGCPIT-EGYGLSETSPVATCNPVDATEFSGTIGLPLPSTEVSIRD-DDGNDLP 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 440 PGhelddhgreRVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaagRNSHRqpwaqgpmy 519
Cdd:PRK07059 403 LG---------EPGEICIRGPQVMAGYWNRPDETAKVMTADG------FFRTGDVGVMDE------RGYTK--------- 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 520 fIQGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGA-VIILDPDhavkWTPLEMRRALKSRLAG 598
Cdd:PRK07059 453 -IVDRKK-DMILVSGFNVYPNEIEEVVASHPGVLEVAAVGVPDEHSGEAVKLfVVKKDPA----LTEEDVKAFCKERLTN 526
|
570 580
....*....|....*....|....*.
gi 573989775 599 YKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK07059 527 YKRPKFVEFRTELPKTNVGKILRREL 552
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
66-626 |
5.32e-21 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 98.49 E-value: 5.32e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKPLTgerVAFLIENSYDYVVPeahsrdvvmFLAV 145
Cdd:PRK12316 3062 LFEEQVERTPDAVALAFG--EQRLSYAELNRRANRLAHRLIERGVGPDVL---VGVAVERSLEMVVG---------LLAI 3127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFA-AKAREVLATDLQQKPvyfelpkhtgssHGAKEAVSLADEDF 224
Cdd:PRK12316 3128 LKAGGAYVPLDPEYPEERLAYMLEDSGAQLLLSQSHLRlPLAQGVQVLDLDRGD------------ENYAEANPAIRTMP 3195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 225 SGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHhIHGTINAVLTPLFAGSSVEFMFPFNAD 304
Cdd:PRK12316 3196 ENLAYVIYTSGSTGKPKGVGIRHSALSNHLCWMQQAYGLGVGDRVLQFTTFS-FDVFVEELFWPLMSGARVVLAGPEDWR 3274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 305 AVwkrfaapflptngTATTAPPISFFTVVPTVYSRLLashktlspQMQAATSEATSPANLRVNISGSAALPTPVKAAWAA 384
Cdd:PRK12316 3275 DP-------------ALLVELINSEGVDVLHAYPSML--------QAFLEEEDAHRCTSLKRIVCGGEALPADLQQQVFA 3333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 385 lsgGNVLLERYGMTEVGM-ALSCGLAFADRVDASVGWPLPSVEARLVDMDtgdviepgheLDDHGRERVGEIQLRGPTVF 463
Cdd:PRK12316 3334 ---GLPLYNLYGPTEATItVTHWQCVEEGKDAVPIGRPIANRACYILDGS----------LEPVPVGALGELYLGGEGLA 3400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 464 REYWRNPDATAKEFVDDGGDGRGRWFKTGDVAIRREP--VGAAGRNSHRqpwaqgpmyfiqgrktadiIKTGGEKVSALE 541
Cdd:PRK12316 3401 RGYHNRPGLTAERFVPDPFVPGERLYRTGDLARYRADgvIEYIGRVDHQ-------------------VKIRGFRIELGE 3461
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 542 VERELLSLPQVAEAAVVAVpsgNWGQKVGAVIILDPDHAVkwtPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINK 621
Cdd:PRK12316 3462 IEARLLEHPWVREAVVLAV---DGRQLVAYVVPEDEAGDL---REALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDR 3535
|
....*
gi 573989775 622 KQLVK 626
Cdd:PRK12316 3536 KALPR 3540
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
62-624 |
6.97e-21 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 96.83 E-value: 6.97e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 62 PRLPIFEAVAAHD--PTSTAVVHALSGRRFTYGELLGDVARARERLREAVDGKplTGERVAFLIENSYDYVVpeahsrdv 139
Cdd:PLN02574 38 PNLDAVSFIFSHHnhNGDTALIDSSTGFSISYSELQPLVKSMAAGLYHVMGVR--QGDVVLLLLPNSVYFPV-------- 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 140 vMFLAVMAARAIAVPLSPPFPAPEL-QYILDQSAASVLVA---SPKFAAKAREVL----ATDLQQKPVYFELPKHTGSSH 211
Cdd:PLN02574 108 -IFLAVLSLGGIVTTMNPSSSLGEIkKRVVDCSVGLAFTSpenVEKLSPLGVPVIgvpeNYDFDSKRIEFPKFYELIKED 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 212 GAKEAVSLADEDFSGAglMLYTSGTTNRPKGVLLPEPVITAQCRSLLQ----AWEYSPADRL-LHVLPLHHIHGTINAVL 286
Cdd:PLN02574 187 FDFVPKPVIKQDDVAA--IMYSSGTTGASKGVVLTHRNLIAMVELFVRfeasQYEYPGSDNVyLAALPMFHIYGLSLFVV 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 287 TPLFAGSSVEFMFPFNADAVWK---RFAapflptngtattappISFFTVVPTVYSRLLASHKTLSpqmqaatseATSPAN 363
Cdd:PLN02574 265 GLLSLGSTIVVMRRFDASDMVKvidRFK---------------VTHFPVVPPILMALTKKAKGVC---------GEVLKS 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 364 LRVNISGSAALPTPVKAAWAALSGGNVLLERYGMTEVGMALSCGLAFAD-RVDASVGWPLPSVEARLVDMDTGDVIEPGH 442
Cdd:PLN02574 321 LKQVSCGAAPLSGKFIQDFVQTLPHVDFIQGYGMTESTAVGTRGFNTEKlSKYSSVGLLAPNMQAKVVDWSTGCLLPPGN 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 443 elddhgrerVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIQ 522
Cdd:PLN02574 401 ---------CGELWIQGPGVMKGYLNNPKATQSTIDKDG------WLRTGDIAYFDE---------------DGYLYIVD 450
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 523 GRKtaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVKWTplEMRRALKSRLAGYKIP 602
Cdd:PLN02574 451 RLK--EIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGSTLSQE--AVINYVAKQVAPYKKV 526
|
570 580
....*....|....*....|..
gi 573989775 603 QVMKIVNHIPRNAMGKINKKQL 624
Cdd:PLN02574 527 RKVVFVQSIPKSPAGKILRREL 548
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
71-626 |
7.26e-21 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 96.67 E-value: 7.26e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHALSG----------RRFTYGELLGDVARARERLREAVDgkPLTGERVAFLIENsydyvVPEahsrdvv 140
Cdd:PRK07867 1 TSSAPTVAELLLPLAEdddrglyfedSFTSWREHIRGSAARAAALRARLD--PTRPPHVGVLLDN-----TPE------- 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 141 mFLAVMAARAIA----VPLSPPFPAPELQYILDQSAASVLVASPKFAAKARE----VLATDLQQKPVYFELPKHTGSSHG 212
Cdd:PRK07867 67 -FSLLLGAAALSgivpVGLNPTRRGAALARDIAHADCQLVLTESAHAELLDGldpgVRVINVDSPAWADELAAHRDAEPP 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 213 AKEAvsLADEDFsgagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAG 292
Cdd:PRK07867 146 FRVA--DPDDLF----MLIFTSGTSGDPKAVRCTHRKVASAGVMLAQRFGLGPDDVCYVSMPLFHSNAVMAGWAVALAAG 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 293 SSVefmfpfnadAVWKRFAAP-FLPTngtaTTAPPISFFTVVPTVYSRLLAShktlsPQMqaaTSEATSPanLRVNISGS 371
Cdd:PRK07867 220 ASI---------ALRRKFSASgFLPD----VRRYGATYANYVGKPLSYVLAT-----PER---PDDADNP--LRIVYGNE 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 372 AAlptPVKAAWAALSGGNVLLERYGMTEVGMALScglAFADRVDASVGWPLPSVEarLVDMDTGDVIEPGhELDDHGR-- 449
Cdd:PRK07867 277 GA---PGDIARFARRFGCVVVDGFGSTEGGVAIT---RTPDTPPGALGPLPPGVA--IVDPDTGTECPPA-EDADGRLln 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 450 --ERVGE-IQLRGPTVFREYWRNPDATAKEFvddggdgRGRWFKTGDVAIRRepvgaagrnshrqpwAQGPMYFiQGRkT 526
Cdd:PRK07867 348 adEAIGElVNTAGPGGFEGYYNDPEADAERM-------RGGVYWSGDLAYRD---------------ADGYAYF-AGR-L 403
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 527 ADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDhaVKWTPLEMRRALKSR--LAGYKIPQV 604
Cdd:PRK07867 404 GDWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPG--AKFDPDAFAEFLAAQpdLGPKQWPSY 481
|
570 580
....*....|....*....|..
gi 573989775 605 MKIVNHIPRNAMGKINKKQLVK 626
Cdd:PRK07867 482 VRVCAELPRTATFKVLKRQLSA 503
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
232-494 |
7.79e-21 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 97.09 E-value: 7.79e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 232 YTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTpLFAGSSVEFmfpFNADaVWKRFa 311
Cdd:PLN02736 228 YTSGTTGTPKGVVLTHGNLIANVAGSSLSTKFYPSDVHISYLPLAHIYERVNQIVM-LHYGVAVGF---YQGD-NLKLM- 301
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 312 apflptNGTATTAPPIsfFTVVPTVYSRLLA----SHKTLSPQMQ-----------AATSEATSPA-------------- 362
Cdd:PLN02736 302 ------DDLAALRPTI--FCSVPRLYNRIYDgitnAVKESGGLKErlfnaaynakkQALENGKNPSpmwdrlvfnkikak 373
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 363 ---NLRVNISGSAALPTPVKAAWAALSGGNVLlERYGMTEVGMALScGLAFADRVDASVGWPLPSVEARLVDmdtgdVIE 439
Cdd:PLN02736 374 lggRVRFMSSGASPLSPDVMEFLRICFGGRVL-EGYGMTETSCVIS-GMDEGDNLSGHVGSPNPACEVKLVD-----VPE 446
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*
gi 573989775 440 PGHELDDHGRERvGEIQLRGPTVFREYWRNPDATaKEFVDDGGdgrgrWFKTGDV 494
Cdd:PLN02736 447 MNYTSEDQPYPR-GEICVRGPIIFKGYYKDEVQT-REVIDEDG-----WLHTGDI 494
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
72-624 |
1.65e-20 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 95.62 E-value: 1.65e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 72 AHDPTSTAVVHALSGRRFTYGELLGDVARARERLRE--AVDGkpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAAR 149
Cdd:PRK05620 22 VHGDTTVTTWGGAEQEQTTFAAIGARAAALAHALHDelGITG----DQRVGSMMYNCAEHLE---------VLFAVACMG 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 150 AIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYFelpkhTGSSHGAKEAVSLA--------- 220
Cdd:PRK05620 89 AVFNPLNKQLMNDQIVHIINHAEDEVIVADPRLAEQLGEILKECPCVRAVVF-----IGPSDADSAAAHMPegikvysye 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 221 -------------DEDFSGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYS--PADRLLHVLPLHHIHG---TI 282
Cdd:PRK05620 164 alldgrstvydwpELDETTAAAICYSTGTTGAPKGVVYSHRSLYLQSLSLRTTDSLAvtHGESFLCCVPIYHVLSwgvPL 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 283 NAVL--TPL-FAGSSVefmfpfnadavwkrfAAPFLpTNGTATTAPPISFFtvVPTVYSRLLASHKTLSPQMQAatseat 359
Cdd:PRK05620 244 AAFMsgTPLvFPGPDL---------------SAPTL-AKIIATAMPRVAHG--VPTLWIQLMVHYLKNPPERMS------ 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 360 spanLRVNISGSAALPTPVKAAWAALSGGNVLlERYGMTE---VGMALS--CGLAFADRVD--ASVGWPLPSVEARLVDm 432
Cdd:PRK05620 300 ----LQEIYVGGSAVPPILIKAWEERYGVDVV-HVWGMTEtspVGTVARppSGVSGEARWAyrVSQGRFPASLEYRIVN- 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 433 dtgdviePGHELDDHGRERvGEIQLRGPTVFREYWRNP----DATAKEF----VDDGG-----DGrgrWFKTGDVAirre 499
Cdd:PRK05620 374 -------DGQVMESTDRNE-GEIQVRGNWVTASYYHSPteegGGAASTFrgedVEDANdrftaDG---WLRTGDVG---- 438
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 500 PVGAAGrnshrqpwaqgpMYFIQGRKtADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDh 579
Cdd:PRK05620 439 SVTRDG------------FLTIHDRA-RDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPG- 504
|
570 580 590 600
....*....|....*....|....*....|....*....|....*...
gi 573989775 580 aVKW---TPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK05620 505 -IEPtreTAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDL 551
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
229-495 |
1.65e-20 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 95.36 E-value: 1.65e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 LMLYTSGTTNRPKGVLLPE----PVITAQCRSLLQAWEysPADRLLHVLPLHHIHgTINAVLTPLFAGSSVEFMFPFN-A 303
Cdd:cd17639 92 CIMYTSGSTGNPKGVMLTHgnlvAGIAGLGDRVPELLG--PDDRYLAYLPLAHIF-ELAAENVCLYRGGTIGYGSPRTlT 168
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 304 DAVWKRfaapflpTNGTATTAPPiSFFTVVPTVYSRL-------LASHKTLSPQM--------QAATSEA-TSPA----- 362
Cdd:cd17639 169 DKSKRG-------CKGDLTEFKP-TLMVGVPAIWDTIrkgvlakLNPMGGLKRTLfwtayqskLKALKEGpGTPLldelv 240
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 363 ----------NLRVNISGSAALPTPVKAaWAALSGGNVLlERYGMTEvgmalSCGLAFADRVD----ASVGWPLPSVEAR 428
Cdd:cd17639 241 fkkvraalggRLRYMLSGGAPLSADTQE-FLNIVLCPVI-QGYGLTE-----TCAGGTVQDPGdletGRVGPPLPCCEIK 313
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 573989775 429 LVDMDtgdviEPGHELD-DHGRervGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVA 495
Cdd:cd17639 314 LVDWE-----EGGYSTDkPPPR---GEILIRGPNVFKGYYKNPEKTKEAFDGDG------WFHTGDIG 367
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
66-626 |
7.07e-20 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 94.84 E-value: 7.07e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREA-VDGKPLTGerVAflIENSYDYVVPeahsrdvvmFLA 144
Cdd:PRK12467 3100 LIEAQVARTPEAPALVFG--DQQLSYAELNRRANRLAHRLIAIgVGPDVLVG--VA--VERSVEMIVA---------LLA 3164
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 145 VMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAK---AREVLATDLQQKpVYFELPKHTGSSHGAKEavSLAd 221
Cdd:PRK12467 3165 VLKAGGAYVPLDPEYPRERLAYMIEDSGVKLLLTQAHLLEQlpaPAGDTALTLDRL-DLNGYSENNPSTRVMGE--NLA- 3240
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 222 edfsgagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHhIHGTINAVLTPLFAGSSVEFmfpf 301
Cdd:PRK12467 3241 -------YVIYTSGSTGKPKGVGVRHGALANHLCWIAEAYELDANDRVLLFMSFS-FDGAQERFLWTLICGGCLVV---- 3308
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 302 NADAVWKRfaapflPTNGTATTAPPISFFTVVPTVYSRLLASHKTlspqmQAATSeatspanLRVNISGSAALPTPVKAA 381
Cdd:PRK12467 3309 RDNDLWDP------EELWQAIHAHRISIACFPPAYLQQFAEDAGG-----ADCAS-------LDIYVFGGEAVPPAAFEQ 3370
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 382 WAALSGGNVLLERYGMTE---VGMALSCGL-AFADRVDASVGWPLPSVEARLVDMDTGDViepghelddhGRERVGEIQL 457
Cdd:PRK12467 3371 VKRKLKPRGLTNGYGPTEavvTVTLWKCGGdAVCEAPYAPIGRPVAGRSIYVLDGQLNPV----------PVGVAGELYI 3440
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 458 RGPTVFREYWRNPDATAKEFVDDGGDGRG-RWFKTGDVAiRREPVGA---AGRNSHRqpwaqgpmyfiqgrktadiIKTG 533
Cdd:PRK12467 3441 GGVGLARGYHQRPSLTAERFVADPFSGSGgRLYRTGDLA-RYRADGVieyLGRIDHQ-------------------VKIR 3500
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 534 GEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVKwtpLEMRRALKSRLAGYKIPQVMKIVNHIPR 613
Cdd:PRK12467 3501 GFRIELGEIEARLLQHPSVREAVVLARDGAGGKQLVAYVVPADPQGDWR---ETLRDHLAASLPDYMVPAQLLVLAAMPL 3577
|
570
....*....|...
gi 573989775 614 NAMGKINKKQLVK 626
Cdd:PRK12467 3578 GPNGKVDRKALPD 3590
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
61-624 |
1.33e-19 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 92.60 E-value: 1.33e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 61 LPRLPIFEAVAAHDPTSTAVVHAlsGRRFTYGEllgDVARAReRLREAVDGKPLT-GERVAFLIENsydyvVP---EAHS 136
Cdd:PLN02479 20 LTPLWFLERAAVVHPTRKSVVHG--SVRYTWAQ---TYQRCR-RLASALAKRSIGpGSTVAVIAPN-----IPamyEAHF 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 137 rDVVMFLAVMAARAIAVPlsppfpAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYFELP----------KH 206
Cdd:PLN02479 89 -GVPMAGAVVNCVNIRLN------APTIAFLLEHSKSEVVMVDQEFFTLAEEALKILAEKKKSSFKPPllivigdptcDP 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 207 TGSSH----GAKEAVSL-------------ADEDFSGAglMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRL 269
Cdd:PLN02479 162 KSLQYalgkGAIEYEKFletgdpefawkppADEWQSIA--LGYTSGTTASPKGVVLHHRGAYLMALSNALIWGMNEGAVY 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 270 LHVLPLHHIHGTINAVLTPLFAGSSVeFMFPFNADAVWkrfaapflptngTATTAPPISFFTVVPTVYSRLLashktlsp 349
Cdd:PLN02479 240 LWTLPMFHCNGWCFTWTLAALCGTNI-CLRQVTAKAIY------------SAIANYGVTHFCAAPVVLNTIV-------- 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 350 qmQAATSEATSPANLRVNISGSAALPTPVKAAWAALSGGNVLlERYGMTEV-GMALSCglAFADRVDAsvgwpLPSVE-A 427
Cdd:PLN02479 299 --NAPKSETILPLPRVVHVMTAGAAPPPSVLFAMSEKGFRVT-HTYGLSETyGPSTVC--AWKPEWDS-----LPPEEqA 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 428 RL--------VDMDTGDVIEP--GHELDDHGRErVGEIQLRGPTVFREYWRNPDATAKEFvddggdgRGRWFKTGDVAIR 497
Cdd:PLN02479 369 RLnarqgvryIGLEGLDVVDTktMKPVPADGKT-MGEIVMRGNMVMKGYLKNPKANEEAF-------ANGWFHSGDLGVK 440
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 498 RepvgaagrnshrqpwaqgPMYFIQGR-KTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILD 576
Cdd:PLN02479 441 H------------------PDGYIEIKdRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESPCAFVTLK 502
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|.
gi 573989775 577 PD---HAVKWTPLEMRRALKSRLAGYKIPQVMkIVNHIPRNAMGKINKKQL 624
Cdd:PLN02479 503 PGvdkSDEAALAEDIMKFCRERLPAYWVPKSV-VFGPLPKTATGKIQKHVL 552
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
71-628 |
2.31e-19 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 91.99 E-value: 2.31e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHALSGRRFTYGELLGDVararERLREAVDGKPLT-GERVAFLIENSydyvvPEAhsrdvvmFLAVMA-A 148
Cdd:PRK05857 24 ARQQPEAIALRRCDGTSALRYRELVAEV----GGLAADLRAQSVSrGSRVLVISDNG-----PET-------YLSVLAcA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 149 R--AIAVPLS---PPFPAPELQYILDQSAASVLVASPKFAAKAREVLATdlqQKPVYFELPKHTGSSHGAKEAVSLADED 223
Cdd:PRK05857 88 KlgAIAVMADgnlPIAAIERFCQITDPAAALVAPGSKMASSAVPEALHS---IPVIAVDIAAVTRESEHSLDAASLAGNA 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 224 FSGAG---LMLYTSGTTNRPKGVLLPEPVITAQ---CRSLLQAW--------EYSPadrllhvLPLHHIhGTINAVLTPL 289
Cdd:PRK05857 165 DQGSEdplAMIFTSGTTGEPKAVLLANRTFFAVpdiLQKEGLNWvtwvvgetTYSP-------LPATHI-GGLWWILTCL 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 290 FAGSS--------VEFMFPFNADAVwkrfaapflptngtATTAppisfftVVPTVYSRLLASHKTlspqmqaatSEATSP 361
Cdd:PRK05857 237 MHGGLcvtggentTSLLEILTTNAV--------------ATTC-------LVPTLLSKLVSELKS---------ANATVP 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 362 ANLRVNISGSAALPTPVKAAWAAlsgGNVLLERYGMTEVGMALSCgLAFAD----RVDA-SVGWPLPSVEARLVDMDTGD 436
Cdd:PRK05857 287 SLRLVGYGGSRAIAADVRFIEAT---GVRTAQVYGLSETGCTALC-LPTDDgsivKIEAgAVGRPYPGVDVYLAATDGIG 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 437 VIEPghelDDHGRERVGEIQLRGPTVFREYWRNPDATaKEFVDDGgdgrgrWFKTGDVAIRREpvgaagrnshrqpwaqG 516
Cdd:PRK05857 363 PTAP----GAGPSASFGTLWIKSPANMLGYWNNPERT-AEVLIDG------WVNTGDLLERRE----------------D 415
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 517 PMYFIQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVII----LDPDHAVKwtpLEMRRAL 592
Cdd:PRK05857 416 GFFYIKGR-SSEMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPDEEFGALVGLAVVasaeLDESAARA---LKHTIAA 491
|
570 580 590
....*....|....*....|....*....|....*...
gi 573989775 593 KSRLAGYKI--PQVMKIVNHIPRNAMGKINKKQLVKAI 628
Cdd:PRK05857 492 RFRRESEPMarPSTIVIVTDIPRTQSGKVMRASLAAAA 529
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
93-626 |
2.52e-19 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 91.76 E-value: 2.52e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 93 ELLGDVAR-ARERLREAVDGKPltGERVAFLIENsydyvVPEAHsrdvVMFLAVMAARAIAVPLSPPFPAPELQYILDQS 171
Cdd:cd05928 45 RELGSLSRkAANVLSGACGLQR--GDRVAVILPR-----VPEWW----LVNVACIRTGLVFIPGTIQLTAKDILYRLQAS 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 172 AASVLVASPKFAAKAREVLAT--DLQQKPV-----------YFELPKHTGSSHGAKEAVSladedfSGAGLMLYTSGTTN 238
Cdd:cd05928 114 KAKCIVTSDELAPEVDSVASEcpSLKTKLLvseksrdgwlnFKELLNEASTEHHCVETGS------QEPMAIYFTSGTTG 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 239 RPKgvllpepvITAQCRSLL--------QAW-EYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSV--EFMFPFNADAVW 307
Cdd:cd05928 188 SPK--------MAEHSHSSLglglkvngRYWlDLTASDIMWNTSDTGWIKSAWSSLFEPWIQGACVfvHHLPRFDPLVIL 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 308 KrfaapflptngTATTAPpISFFTVVPTVYsRLLASHKTLSPQMQAatseatspanLRVNISGSAALPTPVKAAWAALSG 387
Cdd:cd05928 260 K-----------TLSSYP-ITTFCGAPTVY-RMLVQQDLSSYKFPS----------LQHCVTGGEPLNPEVLEKWKAQTG 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 388 GNvLLERYGMTEVGmaLSCGLAFADRVD-ASVGWPLPSVEARLVDmDTGDVIEPGHElddhgrervGEIQLR-GPT---- 461
Cdd:cd05928 317 LD-IYEGYGQTETG--LICANFKGMKIKpGSMGKASPPYDVQIID-DNGNVLPPGTE---------GDIGIRvKPIrpfg 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 462 VFREYWRNPDATAKEFvddggdgRGRWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIqGRkTADIIKTGGEKVSALE 541
Cdd:cd05928 384 LFSGYVDNPEKTAATI-------RGDFYLTGDRGIMDE---------------DGYFWFM-GR-ADDVINSSGYRIGPFE 439
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 542 VERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPD---HAVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGK 618
Cdd:cd05928 440 VESALIEHPAVVESAVVSSPDPIRGEVVKAFVVLAPQflsHDPEQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGK 519
|
....*...
gi 573989775 619 INKKQLVK 626
Cdd:cd05928 520 IQRNELRD 527
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
64-624 |
3.49e-19 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 92.71 E-value: 3.49e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 64 LPIFEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGkplTGERVAFLIENSYDYVVPeahsrdvvmFL 143
Cdd:PRK12316 514 HRLFEEQVERTPEAPALAFG--EETLDYAELNRRANRLAHALIERGVG---PDVLVGVAMERSIEMVVA---------LL 579
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 144 AVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAK---AREVLATDLQQKPVYFElpkhtGSSHGAKEaVSLA 220
Cdd:PRK12316 580 AILKAGGAYVPLDPEYPAERLAYMLEDSGVQLLLSQSHLGRKlplAAGVQVLDLDRPAAWLE-----GYSEENPG-TELN 653
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 221 DEDFSgagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPlhhihgtinavltplfagssvefmFP 300
Cdd:PRK12316 654 PENLA---YVIYTSGSTGKPKGAGNRHRALSNRLCWMQQAYGLGVGDTVLQKTP------------------------FS 706
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 301 FNAdAVWKRFAApfLPTNGTATTAPPISFFTvvPTVYSRLLASHKT----LSPQMQAATSEATSPANL----RVNISGSA 372
Cdd:PRK12316 707 FDV-SVWEFFWP--LMSGARLVVAAPGDHRD--PAKLVELINREGVdtlhFVPSMLQAFLQDEDVASCtslrRIVCSGEA 781
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 373 ALPTPVKAAWAALSGGNvLLERYGMTEVGMALSCGLAFADRVDA-SVGWPLPSVEARLVDMDTGDViePGHELddhgrer 451
Cdd:PRK12316 782 LPADAQEQVFAKLPQAG-LYNLYGPTEAAIDVTHWTCVEEGGDSvPIGRPIANLACYILDANLEPV--PVGVL------- 851
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 452 vGEIQLRGPTVFREYWRNPDATAKEFV-DDGGDGRgRWFKTGDVAIRREP--VGAAGRNSHRqpwaqgpmyfiqgrktad 528
Cdd:PRK12316 852 -GELYLAGRGLARGYHGRPGLTAERFVpSPFVAGE-RMYRTGDLARYRADgvIEYAGRIDHQ------------------ 911
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 529 iIKTGGEKVSALEVERELLSLPQVAEAAVVAVpSGNwgQKVGAVIILDPDHAvkwTPLEMRRALKSRLAGYKIPQVMKIV 608
Cdd:PRK12316 912 -VKLRGLRIELGEIEARLLEHPWVREAAVLAV-DGK--QLVGYVVLESEGGD---WREALKAHLAASLPEYMVPAQWLAL 984
|
570
....*....|....*.
gi 573989775 609 NHIPRNAMGKINKKQL 624
Cdd:PRK12316 985 ERLPLTPNGKLDRKAL 1000
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
116-493 |
4.69e-19 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 90.95 E-value: 4.69e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 116 GERVAFLIENSYDYVVPEahsrdvvmfLAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAA-KAREVLA-TD 193
Cdd:cd17641 36 GDVVAILGDNRPEWVWAE---------LAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIAEDEEQVdKLLEIADrIP 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 194 LQQKPVYFE---LPKH-------------TGSSHGAKEA----VSLADEDFSGAGLMLYTSGTTNRPKGVLLPEPVITAQ 253
Cdd:cd17641 107 SVRYVIYCDprgMRKYddprlisfedvvaLGRALDRRDPglyeREVAAGKGEDVAVLCTTSGTTGKPKLAMLSHGNFLGH 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 254 CRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFM----------------FPFNADAVWKRFAAPflpT 317
Cdd:cd17641 187 CAAYLAADPLGPGDEYVSVLPLPWIGEQMYSVGQALVCGFIVNFPeepetmmedlreigptFVLLPPRVWEGIAAD---V 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 318 NGTATTAPPIS--FFTVVPTVYSRLLASHKTLSPQMQA--------------ATSEATSPANLRVNISGSAALPTPVKAA 381
Cdd:cd17641 264 RARMMDATPFKrfMFELGMKLGLRALDRGKRGRPVSLWlrlaswladallfrPLRDRLGFSRLRSAATGGAALGPDTFRF 343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 382 WAALsgGNVLLERYGMTEvgmalSCGLAFADR---VDA-SVGWPLPSVEARLVDmdtgdviepghelddhgrerVGEIQL 457
Cdd:cd17641 344 FHAI--GVPLKQLYGQTE-----LAGAYTVHRdgdVDPdTVGVPFPGTEVRIDE--------------------VGEILV 396
|
410 420 430
....*....|....*....|....*....|....*.
gi 573989775 458 RGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGD 493
Cdd:cd17641 397 RSPGVFVGYYKNPEATAEDFDEDG------WLHTGD 426
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
68-626 |
7.78e-19 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 90.37 E-value: 7.78e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 68 EAVAAHDPTSTAVVHALSgrRFTYGELLGDV-ARARERLREAVDGkpltGERVAFLIENSydyvvpeahsRDVVMFLAVm 146
Cdd:PRK07788 56 AHAARRAPDRAALIDERG--TLTYAELDEQSnALARGLLALGVRA----GDGVAVLARNH----------RGFVLALYA- 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 147 AARAIA--VPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREvLATDLqqkPVYFELPKHTGSSHGAKEAVSLADEDF 224
Cdd:PRK07788 119 AGKVGAriILLNTGFSGPQLAEVAAREGVKALVYDDEFTDLLSA-LPPDL---GRLRAWGGNPDDDEPSGSTDETLDDLI 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 225 SG------------AGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTpLFAG 292
Cdd:PRK07788 195 AGsstaplpkppkpGGIVILTSGTTGTPKGAPRPEPSPLAPLAGLLSRVPFRAGETTLLPAPMFHATGWAHLTLA-MALG 273
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 293 SSVEFMFPFNADAVWKRFAapflptNGTATTappisfFTVVPTVYSRLLAshktLSPQMQAA--TSeatspaNLR-VNIS 369
Cdd:PRK07788 274 STVVLRRRFDPEATLEDIA------KHKATA------LVVVPVMLSRILD----LGPEVLAKydTS------SLKiIFVS 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 370 GSAALPTPVKAAWAALsgGNVLLERYGMTEVGMAlscglAFADRVD-----ASVGWPLPSVEARLVDmDTGDVIEPGHel 444
Cdd:PRK07788 332 GSALSPELATRALEAF--GPVLYNLYGSTEVAFA-----TIATPEDlaeapGTVGRPPKGVTVKILD-ENGNEVPRGV-- 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 445 ddhgrerVGEIQLRGPTVFREYwrnPDATAKEFVDDggdgrgrWFKTGDVAIrrepVGAAGRnshrqpwaqgpmYFIQGR 524
Cdd:PRK07788 402 -------VGRIFVGNGFPFEGY---TDGRDKQIIDG-------LLSSGDVGY----FDEDGL------------LFVDGR 448
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 525 KTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQV 604
Cdd:PRK07788 449 DD-DMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAPGAAL--DEDAIKDYVRDNLARYKVPRD 525
|
570 580
....*....|....*....|..
gi 573989775 605 MKIVNHIPRNAMGKINKKQLVK 626
Cdd:PRK07788 526 VVFLDELPRNPTGKVLKRELRE 547
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
230-619 |
3.40e-18 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 85.92 E-value: 3.40e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 230 MLYTSGTTNRPKGVLLPEP--VITAQCRSLLQAWEYspADRLLHVLPLHHIHgTINAVLTPLFAGSSVEFMFPFNADAVW 307
Cdd:cd17633 5 IGFTSGTTGLPKAYYRSERswIESFVCNEDLFNISG--EDAILAPGPLSHSL-FLYGAISALYLGGTFIGQRKFNPKSWI 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 308 KRFaapflptNGTATTAppisfFTVVPTVYSRLLASHKTLSPqmqaatseatspanLRVNISGSAALPTPVKAAWAALSG 387
Cdd:cd17633 82 RKI-------NQYNATV-----IYLVPTMLQALARTLEPESK--------------IKSIFSSGQKLFESTKKKLKNIFP 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 388 GNVLLERYGMTEVGMaLSCGLAFADRVDASVGWPLPSVEARLVDMDTGDViepghelddhgrervGEIQLRGPTVFREYw 467
Cdd:cd17633 136 KANLIEFYGTSELSF-ITYNFNQESRPPNSVGRPFPNVEIEIRNADGGEI---------------GKIFVKSEMVFSGY- 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 468 rnpdatakefVDDGGDGRGRWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIqGRKTaDIIKTGGEKVSALEVERELL 547
Cdd:cd17633 199 ----------VRGGFSNPDGWMSVGDIGYVDE---------------EGYLYLV-GRES-DMIIIGGINIFPTEIESVLK 251
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 573989775 548 SLPQVAEAAVVAVPSGNWGQKVGAVIILDpdhavKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKI 619
Cdd:cd17633 252 AIPGIEEAIVVGIPDARFGEIAVALYSGD-----KLTYKQLKRFLKQKLSRYEIPKKIIFVDSLPYTSSGKI 318
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
66-621 |
8.65e-18 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 86.78 E-value: 8.65e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVH---ALSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMF 142
Cdd:cd05970 22 VVDAMAKEYPDKLALVWcddAGEERIFTFAELADYSDKTANFFKAMGIGK---GDTVMLTLKRRYEFWY---------SL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 143 LAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVAS-----PKFAAKAREVLATDLQQKPVYFELPKHTGSSHgakEAV 217
Cdd:cd05970 90 LALHKLGAIAIPATHQLTAKDIVYRIESADIKMIVAIaedniPEEIEKAAPECPSKPKLVWVGDPVPEGWIDFR---KLI 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 218 SLADEDF-------SGAG----LMLYTSGTTNRPKGVllpepvitaqcrsllqawEYSpadrllHVLPLHHI-------- 278
Cdd:cd05970 167 KNASPDFerptansYPCGedilLVYFSSGTTGMPKMV------------------EHD------FTYPLGHIvtakywqn 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 279 ------HGTI------NAVLTPLF----AGSSVeFMFPFnadavwKRFAAPFL----PTNGTATTAPPisfftvvPTVYS 338
Cdd:cd05970 223 vregglHLTVadtgwgKAVWGKIYgqwiAGAAV-FVYDY------DKFDPKALleklSKYGVTTFCAP-------PTIYR 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 339 RLLA---SHKTLSPQMQAATS-EATSPAnlrvnisgsaalptpVKAAWAALSGGNvLLERYGMTEVGMALSCgLAFADRV 414
Cdd:cd05970 289 FLIRedlSRYDLSSLRYCTTAgEALNPE---------------VFNTFKEKTGIK-LMEGFGQTETTLTIAT-FPWMEPK 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 415 DASVGWPLPSVEARLVDMDtGDVIEPGHElddhgrervGEIQLR---GPTV--FREYWRNPDATAKEFVDDggdgrgrWF 489
Cdd:cd05970 352 PGSMGKPAPGYEIDLIDRE-GRSCEAGEE---------GEIVIRtskGKPVglFGGYYKDAEKTAEVWHDG-------YY 414
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 490 KTGDVAIRREpvgaagrnshrqpwaQGPMYFIqGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKV 569
Cdd:cd05970 415 HTGDAAWMDE---------------DGYLWFV-GR-TDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQVV 477
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|...
gi 573989775 570 GAVIILDPDH-AVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINK 621
Cdd:cd05970 478 KATIVLAKGYePSEELKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRR 530
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
141-632 |
1.69e-17 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 86.23 E-value: 1.69e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 141 MFLAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLA------------------TDLQQKPVYFE 202
Cdd:PLN03102 80 MHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFVDRSFEPLAREVLHllssedsnlnlpvifiheIDFPKRPSSEE 159
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 203 LPKHTGSSHGAKEAVSLAD----EDFSGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHI 278
Cdd:PLN03102 160 LDYECLIQRGEPTPSLVARmfriQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHC 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 279 HGTINAVLTPLFAGSSVefmfpfnadaVWKRFAAPFLPTNgtaTTAPPISFFTVVPTVYSRLLASHKT-LSPQmqaatse 357
Cdd:PLN03102 240 NGWTFTWGTAARGGTSV----------CMRHVTAPEIYKN---IEMHNVTHMCCVPTVFNILLKGNSLdLSPR------- 299
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 358 aTSPANLrvnISGSAALPTPVKAAWAALsgGNVLLERYGMTE-VGMALSCglAFADRvdasvgW-PLPS-----VEAR-- 428
Cdd:PLN03102 300 -SGPVHV---LTGGSPPPAALVKKVQRL--GFQVMHAYGLTEaTGPVLFC--EWQDE------WnRLPEnqqmeLKARqg 365
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 429 -----LVDMDTGDViEPGHELDDHGRErVGEIQLRGPTVFREYWRNPDATAKEFvddggdgRGRWFKTGDVairrepvGA 503
Cdd:PLN03102 366 vsilgLADVDVKNK-ETQESVPRDGKT-MGEIVIKGSSIMKGYLKNPKATSEAF-------KHGWLNTGDV-------GV 429
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 504 AGRNSHRQpwaqgpmyfIQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVKW 583
Cdd:PLN03102 430 IHPDGHVE---------IKDR-SKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPCAFVVLEKGETTKE 499
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 584 TPLEMRRALKSRLAGY---KIPQVM---KIV--NHIPRNAMGKINKKQL---VKAIFVEE 632
Cdd:PLN03102 500 DRVDKLVTRERDLIEYcreNLPHFMcprKVVflQELPKNGNGKILKPKLrdiAKGLVVED 559
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
88-624 |
1.80e-17 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 85.96 E-value: 1.80e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 88 RFTYGELLGDVARARERLREavDGKPLtGERVAFLIENSydyvvpeahSRDVVMFLAVMAARAIAVPLSPPFPAPELQYI 167
Cdd:PRK06018 39 RTTYAQIHDRALKVSQALDR--DGIKL-GDRVATIAWNT---------WRHLEAWYGIMGIGAICHTVNPRLFPEQIAWI 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 168 LDQSAASVLVA-----------SPKFAAKAREVLATDLQQKPvyfelPKHTGSSHGAKEAVSLADEDFS-------GAGL 229
Cdd:PRK06018 107 INHAEDRVVITdltfvpilekiADKLPSVERYVVLTDAAHMP-----QTTLKNAVAYEEWIAEADGDFAwktfdenTAAG 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 230 MLYTSGTTNRPKGVLLPE--PVITAQCRSLLQAWEYSPADRLLHVLPLHHIhgtiNAvltplfagssveFMFPFNADAVW 307
Cdd:PRK06018 182 MCYTSGTTGDPKGVLYSHrsNVLHALMANNGDALGTSAADTMLPVVPLFHA----NS------------WGIAFSAPSMG 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 308 KRFAAPFLPTNGTAT----TAPPISFFTVVPTVYSRLLAshktlspQMQAatSEATSPANLRVNISGSAalpTPVKAAWA 383
Cdd:PRK06018 246 TKLVMPGAKLDGASVyellDTEKVTFTAGVPTVWLMLLQ-------YMEK--EGLKLPHLKMVVCGGSA---MPRSMIKA 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 384 ALSGGNVLLERYGMTE---VGMALSCGLAFAD-RVDASV------GWPLPSVEARLVDMDtgdviepGHELDDHGRErVG 453
Cdd:PRK06018 314 FEDMGVEVRHAWGMTEmspLGTLAALKPPFSKlPGDARLdvlqkqGYPPFGVEMKITDDA-------GKELPWDGKT-FG 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 454 EIQLRGPTVFREYWRnpdaTAKEFVDDGGdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYfIQGRkTADIIKTG 533
Cdd:PRK06018 386 RLKVRGPAVAAAYYR----VDGEILDDDG-----FFDTGDVATIDA---------------YGYMR-ITDR-SKDVIKSG 439
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 534 GEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPR 613
Cdd:PRK06018 440 GEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLIVQLKPGETA--TREEILKYMDGKIAKWWMPDDVAFVDAIPH 517
|
570
....*....|.
gi 573989775 614 NAMGKINKKQL 624
Cdd:PRK06018 518 TATGKILKTAL 528
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
88-624 |
1.88e-17 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 85.91 E-value: 1.88e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 88 RFTYGellgDVARARERLREAVDGKPLT-GERVAFLIENSYdyvvpeahsRDVVMFLAVMAARAIAVPLSPPFPAPELQY 166
Cdd:PRK07008 39 RYTYR----DCERRAKQLAQALAALGVEpGDRVGTLAWNGY---------RHLEAYYGVSGSGAVCHTINPRLFPEQIAY 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 167 ILDQSAASVL-----------VASPKFAAKAREVLATDLQQkpvyfeLPKHTGSSHGAKEAVSLADEDF-------SGAG 228
Cdd:PRK07008 106 IVNHAEDRYVlfdltflplvdALAPQCPNVKGWVAMTDAAH------LPAGSTPLLCYETLVGAQDGDYdwprfdeNQAS 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 LMLYTSGTTNRPKGVLLPE--PVITAQCRSLLQAWEYSPADRLLHVLPLHHIhgtiNAVLTPLFA---GSSVEFMFP-FN 302
Cdd:PRK07008 180 SLCYTSGTTGNPKGALYSHrsTVLHAYGAALPDAMGLSARDAVLPVVPMFHV----NAWGLPYSApltGAKLVLPGPdLD 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 303 ADAVWKRFAAPflptngtattapPISFFTVVPTVYSRLLaSHktlspqMQAAtSEATSPANlRVNISGSAALPTPVKAAW 382
Cdd:PRK07008 256 GKSLYELIEAE------------RVTFSAGVPTVWLGLL-NH------MREA-GLRFSTLR-RTVIGGSACPPAMIRTFE 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 383 AALsgGNVLLERYGMTEVG-MALSCGLAFAD---------RVDASVGWPLPSVEARLVDMDtgdviepGHELDDHGRErV 452
Cdd:PRK07008 315 DEY--GVEVIHAWGMTEMSpLGTLCKLKWKHsqlpldeqrKLLEKQGRVIYGVDMKIVGDD-------GRELPWDGKA-F 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 453 GEIQLRGPTVFREYWRNpdaTAKEFVDDggdgrgrWFKTGDVAIrrepVGAAGrnshrqpwaqgpmyFIQ-GRKTADIIK 531
Cdd:PRK07008 385 GDLQVRGPWVIDRYFRG---DASPLVDG-------WFPTGDVAT----IDADG--------------FMQiTDRSKDVIK 436
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 532 TGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHI 611
Cdd:PRK07008 437 SGGEWISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGAEV--TREELLAFYEGKVAKWWIPDDVVFVDAI 514
|
570
....*....|...
gi 573989775 612 PRNAMGKINKKQL 624
Cdd:PRK07008 515 PHTATGKLQKLKL 527
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
90-500 |
2.98e-17 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 85.58 E-value: 2.98e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 90 TYGELLGDVARARERLREAVDGKPltGERVAFLIENSYDYVVPEahsrdvvmfLAVMAARAIAVPLSPPFPAPELQYILD 169
Cdd:cd17632 69 TYAELWERVGAVAAAHDPEQPVRP--GDFVAVLGFTSPDYATVD---------LALTRLGAVSVPLQAGASAAQLAPILA 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 170 QSAASVLVASPKFAAKARE-VLATDLQQKPVYF----ELPKHTGSSHGAKE-------AVSLADEDFSGAG--------- 228
Cdd:cd17632 138 ETEPRLLAVSAEHLDLAVEaVLEGGTPPRLVVFdhrpEVDAHRAALESARErlaavgiPVTTLTLIAVRGRdlppaplfr 217
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 ---------LMLYTSGTTNRPKGVLLPEpvitaqcRSLLQAW--------EYSPADRLLHVLPLHHIHG--TINAVL--- 286
Cdd:cd17632 218 pepdddplaLLIYTSGSTGTPKGAMYTE-------RLVATFWlkvssiqdIRPPASITLNFMPMSHIAGriSLYGTLarg 290
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 287 -TPLFAGSS--------------VE-FMFPFNADAVWKRFAApflptngtattappisfftvvpTVYSRLLASH--KTLS 348
Cdd:cd17632 291 gTAYFAAASdmstlfddlalvrpTElFLVPRVCDMLFQRYQA----------------------ELDRRSVAGAdaETLA 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 349 PQMQAATSEATSPANLRVNISGSAALPTPVKAAWAALSGGNvLLERYGMTEVGMALscglafadrVDASVGWPlPSVEAR 428
Cdd:cd17632 349 ERVKAELRERVLGGRLLAAVCGSAPLSAEMKAFMESLLDLD-LHDGYGSTEAGAVI---------LDGVIVRP-PVLDYK 417
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 573989775 429 LVdmdtgDVIEPGHELDDHGRERvGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREP 500
Cdd:cd17632 418 LV-----DVPELGYFRTDRPHPR-GELLVKTDTLFPGYYKRPEVTAEVFDEDG------FYRTGDVMAELGP 477
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
75-624 |
4.92e-17 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 84.29 E-value: 4.92e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHALSGRRFTYGELLGDVARARERLReavDGKPLTGERVAFLIENSydyvvPEAhsrdVVMFLAVMAARAIAVP 154
Cdd:PRK13390 11 PDRPAVIVAETGEQVSYRQLDDDSAALARVLY---DAGLRTGDVVALLSDNS-----PEA----LVVLWAALRSGLYITA 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 155 LSPPFPAPELQYILDQSAASVLVASPKFAAKAREVlATDLQQKPVYFELPKHTGSSHGAKEAVSLADEDFSGAGLMLYTS 234
Cdd:PRK13390 79 INHHLTAPEADYIVGDSGARVLVASAALDGLAAKV-GADLPLRLSFGGEIDGFGSFEAALAGAGPRLTEQPCGAVMLYSS 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 235 GTTNRPKGVL--LPE--------PVItAQCRSLLQAWE---YSPADRLLHVLPLHHIhGTINAVltplfaGSSVEFMFPF 301
Cdd:PRK13390 158 GTTGFPKGIQpdLPGrdvdapgdPIV-AIARAFYDISEsdiYYSSAPIYHAAPLRWC-SMVHAL------GGTVVLAKRF 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 302 NADAVWkRFAAPFlptngtattapPISFFTVVPTVYSRLLashktlspQMQAATSEATSPANLRVNISGSAALPTPVKAA 381
Cdd:PRK13390 230 DAQATL-GHVERY-----------RITVTQMVPTMFVRLL--------KLDADVRTRYDVSSLRAVIHAAAPCPVDVKHA 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 382 ---WAalsgGNVLLERYGMTEVGmalscGLAFADRVDasvgW-PLPSVEARLVdmdTGDViepgHELDDHGRE----RVG 453
Cdd:PRK13390 290 midWL----GPIVYEYYSSTEAH-----GMTFIDSPD----WlAHPGSVGRSV---LGDL----HICDDDGNElpagRIG 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 454 EIQLRGPTVFREYWRNPDATAKEfvddGGDGRGRWFKTGDVAIRREpvgaagrnshrqpwaQGPMYfIQGRKTADIIkTG 533
Cdd:PRK13390 350 TVYFERDRLPFRYLNDPEKTAAA----QHPAHPFWTTVGDLGSVDE---------------DGYLY-LADRKSFMII-SG 408
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 534 GEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIIL----DPDHavkwtplEMRRAL----KSRLAGYKIPQVM 605
Cdd:PRK13390 409 GVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIQLvegiRGSD-------ELARELidytRSRIAHYKAPRSV 481
|
570
....*....|....*....
gi 573989775 606 KIVNHIPRNAMGKINKKQL 624
Cdd:PRK13390 482 EFVDELPRTPTGKLVKGLL 500
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
204-625 |
5.07e-17 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 84.69 E-value: 5.07e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 204 PKHTGSSHGAKEAVSLADEDFSGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIhgtiN 283
Cdd:PRK13388 129 PAYAELVAAAGALTPHREVDAMDPFMLIFTSGTTGAPKAVRCSHGRLAFAGRALTERFGLTRDDVCYVSMPLFHS----N 204
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 284 AVLT----PLFAGSSVefmfpfnadAVWKRFAAP-FLPT--NGTATtappisFFTVVPTVYSRLLAshktlspqmqaaTS 356
Cdd:PRK13388 205 AVMAgwapAVASGAAV---------ALPAKFSASgFLDDvrRYGAT------YFNYVGKPLAYILA------------TP 257
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 357 EATSPAN--LRVNIsGSAALPTPvKAAWAALSGGNVLlERYGMTEvgmalscGLAFADRVDA----SVGWPLPSVEarLV 430
Cdd:PRK13388 258 ERPDDADnpLRVAF-GNEASPRD-IAEFSRRFGCQVE-DGYGSSE-------GAVIVVREPGtppgSIGRGAPGVA--IY 325
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 431 DMDTGDVIEPGhELDDHGR-----ERVGEI-QLRGPTVFREYWRNPDATAKEFvddggdgRGRWFKTGDVAIRRepvgaa 504
Cdd:PRK13388 326 NPETLTECAVA-RFDAHGAllnadEAIGELvNTAGAGFFEGYYNNPEATAERM-------RHGMYWSGDLAYRD------ 391
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 505 grnshrqpwAQGPMYFiQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHavKWT 584
Cdd:PRK13388 392 ---------ADGWIYF-AGR-TADWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGA--TFD 458
|
410 420 430 440
....*....|....*....|....*....|....*....|...
gi 573989775 585 PLEMRRALKSR--LAGYKIPQVMKIVNHIPRNAMGKINKKQLV 625
Cdd:PRK13388 459 PDAFAAFLAAQpdLGTKAWPRYVRIAADLPSTATNKVLKRELI 501
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
79-624 |
6.40e-17 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 84.26 E-value: 6.40e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 79 AVVHALSGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLSPP 158
Cdd:PLN02330 46 AFVEAVTGKAVTYGEVVRDTRRFAKALRSLGLRK---GQVVVVVLPNVAEYGI---------VALGIMAAGGVFSGANPT 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 159 FPAPELQYILDQSAASVLVASPKFAAKARE------VLATDLQQKPVYFELPKHTGSSHGAKeavsLADEDFSGAGL--M 230
Cdd:PLN02330 114 ALESEIKKQAEAAGAKLIVTNDTNYGKVKGlglpviVLGEEKIEGAVNWKELLEAADRAGDT----SDNEEILQTDLcaL 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 231 LYTSGTTNRPKGVLLPEPVITAQ-CRSLLQAW-EYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNadavwk 308
Cdd:PLN02330 190 PFSSGTTGISKGVMLTHRNLVANlCSSLFSVGpEMIGQVVTLGLIPFFHIYGITGICCATLRNKGKVVVMSRFE------ 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 309 rfaapfLPTNGTATTAPPISFFTVVPTVYSRLLAShktlsPQMQAATseaTSPANLRVNISGSAALPTPVKAAWAALSGG 388
Cdd:PLN02330 264 ------LRTFLNALITQEVSFAPIVPPIILNLVKN-----PIVEEFD---LSKLKLQAIMTAAAPLAPELLTAFEAKFPG 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 389 NVLLERYGMTEvgmaLSC----------GLAFADRvdASVGWPLPSVEARLVDMDTGDVIEpghelddhgRERVGEIQLR 458
Cdd:PLN02330 330 VQVQEAYGLTE----HSCitlthgdpekGHGIAKK--NSVGFILPNLEVKFIDPDTGRSLP---------KNTPGELCVR 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 459 GPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIQgrKTADIIKTGGEKVS 538
Cdd:PLN02330 395 SQCVMQGYYNNKEETDRTIDEDG------WLHTGDIGYIDD---------------DGDIFIVD--RIKELIKYKGFQVA 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 539 ALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDhaVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGK 618
Cdd:PLN02330 452 PAELEAILLTHPSVEDAAVVPLPDEEAGEIPAACVVINPK--AKESEEDILNFVAANVAHYKKVRVVQFVDSIPKSLSGK 529
|
....*.
gi 573989775 619 INKKQL 624
Cdd:PLN02330 530 IMRRLL 535
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
229-624 |
6.65e-17 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 84.04 E-value: 6.65e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 LMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVefmfpfnadaVWK 308
Cdd:PRK13382 200 VILLTSGTTGTPKGARRSGPGGIGTLKAILDRTPWRAEEPTVIVAPMFHAWGFSQLVLAASLACTIV----------TRR 269
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 309 RF---AAPFLPTNGTATTappisfFTVVPTVYSRLL-ASHKTLSPQMQAATSEATSpanlrvniSGSAALPTPVKAAWAA 384
Cdd:PRK13382 270 RFdpeATLDLIDRHRATG------LAVVPVMFDRIMdLPAEVRNRYSGRSLRFAAA--------SGSRMRPDVVIAFMDQ 335
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 385 LsgGNVLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDMDTGDViePGHElddhgrerVGEIQLRGPTVFR 464
Cdd:PRK13382 336 F--GDVIYNNYNATEAGMIATATPADLRAAPDTAGRPAEGTEIRILDQDFREV--PTGE--------VGTIFVRNDTQFD 403
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 465 EYwrnPDATAKEFVDDggdgrgrWFKTGDVAIRREpvgaAGRnshrqpwaqgpmYFIQGRKTaDIIKTGGEKVSALEVER 544
Cdd:PRK13382 404 GY---TSGSTKDFHDG-------FMASGDVGYLDE----NGR------------LFVVGRDD-EMIVSGGENVYPIEVEK 456
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 545 ELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK13382 457 TLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKPGASA--TPETLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRREL 534
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
71-624 |
7.89e-17 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 83.89 E-value: 7.89e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVH---ALSGRrftygellgDVARARERL-REAVDGKPLTGERVAFLIENSYDYVVPeahsrdvvmFLAVM 146
Cdd:PRK13383 45 AARWPGRTAIIDddgALSYR---------ELQRATESLaRRLTRDGVAPGRAVGVMCRNGRGFVTA---------VFAVG 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 147 AARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKareVLATDlqQKPVYFELPKHTGSSHGAKEAVSladedfSG 226
Cdd:PRK13383 107 LLGADVVPISTEFRSDALAAALRAHHISTVVADNEFAER---IAGAD--DAVAVIDPATAGAEESGGRPAVA------AP 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 227 AGLMLYTSGTTNRPKGVLLPEPVITAQCRS--LLQAWEYSPADRLLHVLPLHHIHGTINAVLTpLFAGSSVEFMFPFNAD 304
Cdd:PRK13383 176 GRIVLLTSGTTGKPKGVPRAPQLRSAVGVWvtILDRTRLRTGSRISVAMPMFHGLGLGMLMLT-IALGGTVLTHRHFDAE 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 305 AVWKRfaapflptngtaTTAPPISFFTVVPTVYSRLLashkTLSPQMQAatseaTSP-ANLRVNISGSAALpTPVKAAWA 383
Cdd:PRK13383 255 AALAQ------------ASLHRADAFTAVPVVLARIL----ELPPRVRA-----RNPlPQLRVVMSSGDRL-DPTLGQRF 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 384 ALSGGNVLLERYGMTEVGMAlscGLAF-ADRVDA--SVGWPLPSVEARLVDMDTGDViepghelddhgrervgeiqlrGP 460
Cdd:PRK13383 313 MDTYGDILYNGYGSTEVGIG---ALATpADLRDApeTVGKPVAGCPVRILDRNNRPV---------------------GP 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 461 TVFREYWRNPDATAKEFVDDGG----DGRGrwfKTGDVAIRREpvgaAGRnshrqpwaqgpmYFIQGRKTaDIIKTGGEK 536
Cdd:PRK13383 369 RVTGRIFVGGELAGTRYTDGGGkavvDGMT---STGDMGYLDN----AGR------------LFIVGRED-DMIISGGEN 428
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 537 VSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVKWTplEMRRALKSRLAGYKIPQVMKIVNHIPRNAM 616
Cdd:PRK13383 429 VYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHPGSGVDAA--QLRDYLKDRVSRFEQPRDINIVSSIPRNPT 506
|
....*...
gi 573989775 617 GKINKKQL 624
Cdd:PRK13383 507 GKVLRKEL 514
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
86-624 |
1.09e-16 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 83.72 E-value: 1.09e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 86 GRRFTYGELLGDVARARERLREAVDGKPltGERVAFLIENSYDYvvpeahsrDVVMFLAvMAARAIAVPLSPPFPAPELQ 165
Cdd:PRK12492 47 GVTLSYAELERHSAAFAAYLQQHTDLVP--GDRIAVQMPNVLQY--------PIAVFGA-LRAGLIVVNTNPLYTAREMR 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 166 YILDQSAASVLVASPKFAAKAREVLA-TDLQ--------------------------QKPV-YFELPK--------HTGS 209
Cdd:PRK12492 116 HQFKDSGARALVYLNMFGKLVQEVLPdTGIEylieakmgdllpaakgwlvntvvdkvKKMVpAYHLPQavpfkqalRQGR 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 210 SHGAKeAVSLADEDFSgagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADR----------LLHVLPLHHIH 279
Cdd:PRK12492 196 GLSLK-PVPVGLDDIA---VLQYTGGTTGLAKGAMLTHGNLVANMLQVRACLSQLGPDGqplmkegqevMIAPLPLYHIY 271
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 280 G-TINAVLTPLFAGSSVEFMFPFNADAVWK-----RFAApFLPTNgtattappisfftvvpTVYSRLLAShktlsPQMQA 353
Cdd:PRK12492 272 AfTANCMCMMVSGNHNVLITNPRDIPGFIKelgkwRFSA-LLGLN----------------TLFVALMDH-----PGFKD 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 354 ATSeatspANLRVNISGSAALPTPVKAAWAALSGGNVLlERYGMTEVGmALSCGLAFADRVD-ASVGWPLPSVEARLVDm 432
Cdd:PRK12492 330 LDF-----SALKLTNSGGTALVKATAERWEQLTGCTIV-EGYGLTETS-PVASTNPYGELARlGTVGIPVPGTALKVID- 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 433 DTGDVIEPGhelddhgrERvGEIQLRGPTVFREYWRNPDATAkEFVDDGGdgrgrWFKTGDVAIrrepVGAAGRNShrqp 512
Cdd:PRK12492 402 DDGNELPLG--------ER-GELCIKGPQVMKGYWQQPEATA-EALDAEG-----WFKTGDIAV----IDPDGFVR---- 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 513 waqgpmyfIQGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGA-VIILDPDHAVKwtplEMRRA 591
Cdd:PRK12492 459 --------IVDRKK-DLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLfVVARDPGLSVE----ELKAY 525
|
570 580 590
....*....|....*....|....*....|...
gi 573989775 592 LKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK12492 526 CKENFTGYKVPKHIVLRDSLPMTPVGKILRREL 558
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
75-624 |
3.04e-16 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 81.74 E-value: 3.04e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHAlsGRRFTYGELLGDVARARERLRE-AVDGKPLtgerVAFLIENSYDYVVPeahsrdvvmFLAVMAARAIAV 153
Cdd:cd17650 1 PDAIAVSDA--TRQLTYRELNERANQLARTLRGlGVAPGSV----VGVCADRSLDAIVG---------LLAVLKAGGAYV 65
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 154 PLSPPFPAPELQYILDQSAASVLVASPkfaakarevlatdlqQKPVYfelpkhtgsshgakeavsladedfsgaglMLYT 233
Cdd:cd17650 66 PIDPDYPAERLQYMLEDSGAKLLLTQP---------------EDLAY-----------------------------VIYT 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 234 SGTTNRPKGVLlpepVITAQCRSLLQAWeyspaDRLLHVLPLHHIHGTINAVLTPLFAGSsvefmfpfnadavwkrFAAP 313
Cdd:cd17650 102 SGTTGKPKGVM----VEHRNVAHAAHAW-----RREYELDSFPVRLLQMASFSFDVFAGD----------------FARS 156
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 314 FLpTNGTATTAP------PISFFTVVPTVYSRLLASHKTLS-PQMQAATSEATSPANLRVNISGSAALPTP-VKAAWAAL 385
Cdd:cd17650 157 LL-NGGTLVICPdevkldPAALYDLILKSRITLMESTPALIrPVMAYVYRNGLDLSAMRLLIVGSDGCKAQdFKTLAARF 235
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 386 SGGNVLLERYGMTEV---------GMALSCGLAFAdrvdaSVGWPLPSVeaRLVDMDTGDVIEPGHElddhgrerVGEIQ 456
Cdd:cd17650 236 GQGMRIINSYGVTEAtidstyyeeGRDPLGDSANV-----PIGRPLPNT--AMYVLDERLQPQPVGV--------AGELY 300
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 457 LRGPTVFREYWRNPDATAKEFVDDGGDGRGRWFKTGDVAiRREPVGAA---GRNSHRqpwaqgpmyfiqgrktadiIKTG 533
Cdd:cd17650 301 IGGAGVARGYLNRPELTAERFVENPFAPGERMYRTGDLA-RWRADGNVellGRVDHQ-------------------VKIR 360
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 534 GEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIIldPDHAVKWTplEMRRALKSRLAGYKIPQVMKIVNHIPR 613
Cdd:cd17650 361 GFRIELGEIESQLARHPAIDEAVVAVREDKGGEARLCAYVV--AAATLNTA--ELRAFLAKELPSYMIPSYYVQLDALPL 436
|
570
....*....|.
gi 573989775 614 NAMGKINKKQL 624
Cdd:cd17650 437 TPNGKVDRRAL 447
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
231-624 |
3.65e-16 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 82.66 E-value: 3.65e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 231 LYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAG-SSVEFMFPFNADAVWK- 308
Cdd:PRK08633 788 IFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVILSSLPFFHSFGLTVTLWLPLLEGiKVVYHPDPTDALGIAKl 867
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 309 --RFAAPFLPtnGTATtappisFFtvvptvysRLLASHKTLSPQMQAatseatspaNLRVNISGSAALPTPVKAAWAaLS 386
Cdd:PRK08633 868 vaKHRATILL--GTPT------FL--------RLYLRNKKLHPLMFA---------SLRLVVAGAEKLKPEVADAFE-EK 921
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 387 GGNVLLERYGMTEVGMALSCGL--AFADRVD-------ASVGWPLPSVEARLVDMDTGDVIEPGHElddhgrervGEIQL 457
Cdd:PRK08633 922 FGIRILEGYGATETSPVASVNLpdVLAADFKrqtgskeGSVGMPLPGVAVRIVDPETFEELPPGED---------GLILI 992
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 458 RGPTVFREYWRNPDATAKEFVDDGGDgrgRWFKTGDVAIRREpvgaagrnshrqpwaQGpMYFIQGRkTADIIKTGGEKV 537
Cdd:PRK08633 993 GGPQVMKGYLGDPEKTAEVIKDIDGI---GWYVTGDKGHLDE---------------DG-FLTITDR-YSRFAKIGGEMV 1052
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 538 SALEVERELLSLpqvaeaavvavpSGNWGQKVGAVIIldPDH------AVKWTPLEMRRA-LKSRLAGYKIPQVMK---- 606
Cdd:PRK08633 1053 PLGAVEEELAKA------------LGGEEVVFAVTAV--PDEkkgeklVVLHTCGAEDVEeLKRAIKESGLPNLWKpsry 1118
|
410
....*....|....*....
gi 573989775 607 -IVNHIPRNAMGKINKKQL 624
Cdd:PRK08633 1119 fKVEALPLLGSGKLDLKGL 1137
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
71-635 |
6.54e-16 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 82.01 E-value: 6.54e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAvDGKPltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARA 150
Cdd:PRK10252 468 AAKTPDAPALADA--RYQFSYREMREQVVALANLLRER-GVKP--GDSVAVALPRSVFLTL---------ALHAIVEAGA 533
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 151 IAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVyfELPkhtgsshgAKEAVSLADEDFSGAGLM 230
Cdd:PRK10252 534 AWLPLDTGYPDDRLKMMLEDARPSLLITTADQLPRFADVPDLTSLCYNA--PLA--------PQGAAPLQLSQPHHTAYI 603
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 231 LYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHhihgtinavltplFAGSSVEFMFPFNADAvwkrf 310
Cdd:PRK10252 604 IFTSGSTGRPKGVMVGQTAIVNRLLWMQNHYPLTADDVVLQKTPCS-------------FDVSVWEFFWPFIAGA----- 665
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 311 aapflptngTATTAPPISFFTvvPTVYSRLLASHKTLS----PQMQAATSEATSPANL--------RVNISGSaALPTPV 378
Cdd:PRK10252 666 ---------KLVMAEPEAHRD--PLAMQQFFAEYGVTTthfvPSMLAAFVASLTPEGArqscaslrQVFCSGE-ALPADL 733
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 379 KAAWAALSGGNvLLERYGMTEVGMALS----CGLAFADRVDASV--GWPLpsvearlvdMDTGDVIepgheLDDHGRER- 451
Cdd:PRK10252 734 CREWQQLTGAP-LHNLYGPTEAAVDVSwypaFGEELAAVRGSSVpiGYPV---------WNTGLRI-----LDARMRPVp 798
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 452 ---VGEIQLRGPTVFREYWRNPDATAKEFVDDGGDGRGRWFKTGDVAiRREPVGAA---GRNSHRqpwaqgpmyfiqgrk 525
Cdd:PRK10252 799 pgvAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVA-RWLDDGAVeylGRSDDQ--------------- 862
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 526 tadiIKTGGEKVSALEVERELLSLPQVAEAAV-------VAVPSGNWGQKVGAVIildPDHAVKWTPLEMRRALKSRLAG 598
Cdd:PRK10252 863 ----LKIRGQRIELGEIDRAMQALPDVEQAVThacvinqAAATGGDARQLVGYLV---SQSGLPLDTSALQAQLRERLPP 935
|
570 580 590
....*....|....*....|....*....|....*..
gi 573989775 599 YKIPQVMKIVNHIPRNAMGKINKKQLVKAIFVEETSG 635
Cdd:PRK10252 936 HMVPVVLLQLDQLPLSANGKLDRKALPLPELKAQVPG 972
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
272-628 |
9.84e-16 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 80.04 E-value: 9.84e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 272 VLPLHHIHGTINAV---LT--PLFAGSsvefmfpfnadavWKRFaapflpTNGTATTAPPISFFT-VVPTVYSRLLASHk 345
Cdd:PRK07445 166 VLPLYHVSGLMQFMrsfLTggKLVILP-------------YKRL------KSGQELPPNPSDFFLsLVPTQLQRLLQLR- 225
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 346 tlspqmqaatseATSPANLR-VNISGSAALPTPV-KAAWAALSggnvLLERYGMTEVGmALSCGLAFAD--RVDASVGWP 421
Cdd:PRK07445 226 ------------PQWLAQFRtILLGGAPAWPSLLeQARQLQLR----LAPTYGMTETA-SQIATLKPDDflAGNNSSGQV 288
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 422 LPSVEARLVDMDTGdviepghelddhgrervgEIQLRGPTVFREYWRNPDATAKEF-VDDggdgRGRWFktgdvairrep 500
Cdd:PRK07445 289 LPHAQITIPANQTG------------------NITIQAQSLALGYYPQILDSQGIFeTDD----LGYLD----------- 335
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 501 vgaagrnshrqpwAQGPMYfIQGRKTADIIkTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHA 580
Cdd:PRK07445 336 -------------AQGYLH-ILGRNSQKII-TGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDPSI 400
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 573989775 581 vkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLVKAI 628
Cdd:PRK07445 401 ---SLEELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQIA 445
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
87-500 |
1.04e-15 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 80.55 E-value: 1.04e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 87 RRFTYGELLgdvaRARERLREAVDGKPLTGER-VAFLIENSYDyvvpeaHSRdvvMFLAVMAARAIAVPLSPPFPA---- 161
Cdd:cd05921 24 RRVTYAEAL----RQVRAIAQGLLDLGLSAERpLLILSGNSIE------HAL---MALAAMYAGVPAAPVSPAYSLmsqd 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 162 -PELQYILDQSAASVLVAS--PKFAAKAREVLATDLQQKPVYFELPKHTGSSHG---AKEAVSLADEDFSGAG-----LM 230
Cdd:cd05921 91 lAKLKHLFELLKPGLVFAQdaAPFARALAAIFPLGTPLVVSRNAVAGRGAISFAelaATPPTAAVDAAFAAVGpdtvaKF 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 231 LYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYsPADR---LLHVLPLHHIhgtinavltplFAGSSVEFMFPFNADAVW 307
Cdd:cd05921 171 LFTSGSTGLPKAVINTQRMLCANQAMLEQTYPF-FGEEppvLVDWLPWNHT-----------FGGNHNFNLVLYNGGTLY 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 308 KRFAAPfLPtNGTATTAPPISffTVVPTVYSRLLASHKTLSPQM-QAATSEATSPANLRVNISGSAALPTPVKAAWAALS 386
Cdd:cd05921 239 IDDGKP-MP-GGFEETLRNLR--EISPTVYFNVPAGWEMLVAALeKDEALRRRFFKRLKLMFYAGAGLSQDVWDRLQALA 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 387 GGNV-----LLERYGMTEVGmALSCGLAFADRVDASVGWPLPSVEARLVDMDtgdviepghelddhGRErvgEIQLRGPT 461
Cdd:cd05921 315 VATVgeripMMAGLGATETA-PTATFTHWPTERSGLIGLPAPGTELKLVPSG--------------GKY---EVRVKGPN 376
|
410 420 430
....*....|....*....|....*....|....*....
gi 573989775 462 VFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREP 500
Cdd:cd05921 377 VTPGYWRQPELTAQAFDEEG------FYCLGDAAKLADP 409
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
116-499 |
2.64e-15 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 80.21 E-value: 2.64e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 116 GERVAFLIENSYDYVVPeahsrdvvmFLAVMAARAIAVPLSPPFPA----------------PELQYILDQSAASVLVAS 179
Cdd:PRK05691 64 GDRAVLLFPSGPDYVAA---------FFGCLYAGVIAVPAYPPESArrhhqerllsiiadaePRLLLTVADLRDSLLQME 134
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 180 PKFAAKAREVLATDlQQKPVYFElpkhtgsshgAKEAVSLADEDFSgagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQ 259
Cdd:PRK05691 135 ELAAANAPELLCVD-TLDPALAE----------AWQEPALQPDDIA---FLQYTSGSTALPKGVQVSHGNLVANEQLIRH 200
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 260 AW--EYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFP--FNADAVwkRFAAPFLPTNGTATTAPPISFftvvpt 335
Cdd:PRK05691 201 GFgiDLNPDDVIVSWLPLYHDMGLIGGLLQPIFSGVPCVLMSPayFLERPL--RWLEAISEYGGTISGGPDFAY------ 272
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 336 vysRLLashktlSPQMQAATSEATSPANLRVNISGSAALPTPVKAAWA---ALSG--GNVLLERYGMTEVGMALSCGL-- 408
Cdd:PRK05691 273 ---RLC------SERVSESALERLDLSRWRVAYSGSEPIRQDSLERFAekfAACGfdPDSFFASYGLAEATLFVSGGRrg 343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 409 -----------AFA-DRVDA-------SVGWPLPSVEARLVDMDTGDViepgheLDDHgreRVGEIQLRGPTVFREYWRN 469
Cdd:PRK05691 344 qgipaleldaeALArNRAEPgtgsvlmSCGRSQPGHAVLIVDPQSLEV------LGDN---RVGEIWASGPSIAHGYWRN 414
|
410 420 430
....*....|....*....|....*....|
gi 573989775 470 PDATAKEFVDDggDGRgRWFKTGDVAIRRE 499
Cdd:PRK05691 415 PEASAKTFVEH--DGR-TWLRTGDLGFLRD 441
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
397-627 |
2.81e-15 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 79.17 E-value: 2.81e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 397 MTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDmDTGDVIEPGhelddhgreRVGEIQLRG--PTVFREYWRNPDATA 474
Cdd:PRK04319 357 MTETGGIMIANYPAMDIKPGSMGKPLPGIEAAIVD-DQGNELPPN---------RMGNLAIKKgwPSMMRGIWNNPEKYE 426
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 475 KEFVDDggdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFiQGRKTaDIIKTGGEKVSALEVERELLSLPQVAE 554
Cdd:PRK04319 427 SYFAGD-------WYVSGDSAYMDE---------------DGYFWF-QGRVD-DVIKTSGERVGPFEVESKLMEHPAVAE 482
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 573989775 555 AAVVAVPSGNWGQKVGAVIIL----DPDHAVKwtpLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKqLVKA 627
Cdd:PRK04319 483 AGVIGKPDPVRGEIIKAFVALrpgyEPSEELK---EEIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRR-VLKA 555
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
230-494 |
2.93e-15 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 79.12 E-value: 2.93e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 230 MLYTSGTTNRPKGVLLPEPVITAQCRS---LLQAWEYSPA--DRLLHVLPLHHIHGTINAVLTpLFAGSSVEFmfpFNAD 304
Cdd:PLN02861 225 IMYTSGTTGEPKGVILTNRAIIAEVLStdhLLKVTDRVATeeDSYFSYLPLAHVYDQVIETYC-ISKGASIGF---WQGD 300
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 305 AvwkRFAapflpTNGTATTAPPIsfFTVVPTVYSRL--------------------------LASHKTLSPQMQAA---- 354
Cdd:PLN02861 301 I---RYL-----MEDVQALKPTI--FCGVPRVYDRIytgimqkissggmlrkklfdfaynykLGNLRKGLKQEEASprld 370
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 355 ------TSEATSpANLRVNISGSAALPTPVKAaWAALSGGNVLLERYGMTEvgmalSCGLAFADRVD-----ASVGWPLP 423
Cdd:PLN02861 371 rlvfdkIKEGLG-GRVRLLLSGAAPLPRHVEE-FLRVTSCSVLSQGYGLTE-----SCGGCFTSIANvfsmvGTVGVPMT 443
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 573989775 424 SVEARLVDmdtgdVIEPGHE-LDDHGRervGEIQLRGPTVFREYWRNPDATAKEFVDDggdgrgrWFKTGDV 494
Cdd:PLN02861 444 TIEARLES-----VPEMGYDaLSDVPR---GEICLRGNTLFSGYHKRQDLTEEVLIDG-------WFHTGDI 500
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
232-544 |
4.28e-15 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 78.30 E-value: 4.28e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 232 YTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMfpfnadavwkrfa 311
Cdd:cd05908 113 FSSGSTGDPKGVMLTHENLVHNMFAILNSTEWKTKDRILSWMPLTHDMGLIAFHLAPLIAGMNQYLM------------- 179
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 312 apflptngtattapPISFFTVVPTVYSRLLASHK---TLSP---------QMQAATSEATSPANLRVNISGSAA-LPTPV 378
Cdd:cd05908 180 --------------PTRLFIRRPILWLKKASEHKatiVSSPnfgykyflkTLKPEKANDWDLSSIRMILNGAEPiDYELC 245
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 379 KAAWAALSG----GNVLLERYGMTEVGMALSC---------------GLAFADRVDA------------SVGWPLPSVEA 427
Cdd:cd05908 246 HEFLDHMSKyglkRNAILPVYGLAEASVGASLpkaqspfktitlgrrHVTHGEPEPEvdkkdsecltfvEVGKPIDETDI 325
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 428 RLVDmdtgdviEPGHELDDhgrERVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRREpvgaagrn 507
Cdd:cd05908 326 RICD-------EDNKILPD---GYIGHIQIRGKNVTPGYYNNPEATAKVFTDDG------WLKTGDLGFIRN-------- 381
|
330 340 350
....*....|....*....|....*....|....*..
gi 573989775 508 shrqpwaqGPMYfIQGRKTaDIIKTGGEKVSALEVER 544
Cdd:cd05908 382 --------GRLV-ITGREK-DIIFVNGQNVYPHDIER 408
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
365-624 |
5.51e-15 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 78.11 E-value: 5.51e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 365 RVNIsgsAALPTPVKAAW---AALSGGNVLLERYGMTEVGMAlSCGLAFADRVDASVGWPLPSV---------EARLVDM 432
Cdd:PRK10946 272 QVNV---TALVPPAVSLWlqaIAEGGSRAQLASLKLLQVGGA-RLSETLARRIPAELGCQLQQVfgmaeglvnYTRLDDS 347
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 433 DT------GDVIEPGHE---LDDHGRE----RVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVAIRRE 499
Cdd:PRK10946 348 DErifttqGRPMSPDDEvwvADADGNPlpqgEVGRLMTRGPYTFRGYYKSPQHNASAFDANG------FYCSGDLVSIDP 421
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 500 pvgaagrnshrqpwaQGpmYF-IQGRKTaDIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIIL-DP 577
Cdd:PRK10946 422 ---------------DG--YItVVGREK-DQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVkEP 483
|
250 260 270 280
....*....|....*....|....*....|....*....|....*...
gi 573989775 578 dhaVKwtPLEMRRALKSR-LAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK10946 484 ---LK--AVQLRRFLREQgIAEFKLPDRVECVDSLPLTAVGKVDKKQL 526
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
139-624 |
6.59e-15 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 77.48 E-value: 6.59e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 139 VVMFLAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPkfaakarEVLAtdlqqkpvyfelpkhtgsshgakeavs 218
Cdd:cd17644 64 IIGLLAILKAGGAYVPLDPNYPQERLTYILEDAQISVLLTQP-------ENLA--------------------------- 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 219 ladedfsgagLMLYTSGTTNRPKGVLLpepvitaQCRSLLqaweyspadrllhvlplHHIHGTINAV--LTPLFAGSSVE 296
Cdd:cd17644 110 ----------YVIYTSGSTGKPKGVMI-------EHQSLV-----------------NLSHGLIKEYgiTSSDRVLQFAS 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 297 FMFPFNADAVWkrfaaPFLPTNGTATTAPPISFFTV----------------VPTVYSRLLASHKTLSpqmqaatsEATS 360
Cdd:cd17644 156 IAFDVAAEEIY-----VTLLSGATLVLRPEEMRSSLedfvqyiqqwqltvlsLPPAYWHLLVLELLLS--------TIDL 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 361 PANLR-VNISGSAALPTPVkAAWAALSGGNV-LLERYGMTEVGMALSC----GLAFADRVDASVGWPLPSVEARLVDmDT 434
Cdd:cd17644 223 PSSLRlVIVGGEAVQPELV-RQWQKNVGNFIqLINVYGPTEATIAATVcrltQLTERNITSVPIGRPIANTQVYILD-EN 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 435 GDVIEPGhelddhgreRVGEIQLRGPTVFREYWRNPDATAKEFVDD--GGDGRGRWFKTGDVAiRREPVGAA---GRNSH 509
Cdd:cd17644 301 LQPVPVG---------VPGELHIGGVGLARGYLNRPELTAEKFISHpfNSSESERLYKTGDLA-RYLPDGNIeylGRIDN 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 510 RqpwaqgpmyfiqgrktadiIKTGGEKVSALEVERELLSLPQVAEAAVVAV--PSGNwgQKVGAVIIldPDHAVKWTPLE 587
Cdd:cd17644 371 Q-------------------VKIRGFRIELGEIEAVLSQHNDVKTAVVIVRedQPGN--KRLVAYIV--PHYEESPSTVE 427
|
490 500 510
....*....|....*....|....*....|....*..
gi 573989775 588 MRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd17644 428 LRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
66-624 |
1.01e-14 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 76.82 E-value: 1.01e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVHalSGRRFTYGELLGDVARARERLREAvDGKPltGERVAFLIENSYDYVVPeahsrdvvmFLAV 145
Cdd:cd17645 3 LFEEQVERTPDHVAVVD--RGQSLTYKQLNEKANQLARHLRGK-GVKP--DDQVGIMLDKSLDMIAA---------ILGV 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKfaakarevlatDLqqkpVYfelpkhtgsshgakeavsladedfs 225
Cdd:cd17645 69 LKAGGAYVPIDPDYPGERIAYMLADSSAKILLTNPD-----------DL----AY------------------------- 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 226 gaglMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRllhvlplhhihgtinavltplfagSSVEFMFPFNAdA 305
Cdd:cd17645 109 ----VIYTSGSTGLPKGVMIEHHNLVNLCEWHRPYFGVTPADK------------------------SLVYASFSFDA-S 159
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 306 VWKRFaaPFLPTNGTATTAPPISFFTVVpTVYSRLLASHKTLS--PQMQAATSEATSPANLRVNISGSAALPTPVKAAWA 383
Cdd:cd17645 160 AWEIF--PHLTAGAALHVVPSERRLDLD-ALNDYFNQEGITISflPTGAAEQFMQLDNQSLRVLLTGGDKLKKIERKGYK 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 384 alsggnvLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVeaRLVDMDTGDVIEPghelddhgRERVGEIQLRGPTVF 463
Cdd:cd17645 237 -------LVNNYGPTENTVVATSFEIDKPYANIPIGKPIDNT--RVYILDEALQLQP--------IGVAGELCIAGEGLA 299
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 464 REYWRNPDATAKEFVDDGGDGRGRWFKTGDVAiRREPvgaagrnshrqpwaQGPMYFIqGRkTADIIKTGGEKVSALEVE 543
Cdd:cd17645 300 RGYLNRPELTAEKFIVHPFVPGERMYRTGDLA-KFLP--------------DGNIEFL-GR-LDQQVKIRGYRIEPGEIE 362
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 544 RELLSLPQVAEAAVVAVPSGNwGQKVGAVIILDPDHAvkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQ 623
Cdd:cd17645 363 PFLMNHPLIELAAVLAKEDAD-GRKYLVAYVTAPEEI---PHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKA 438
|
.
gi 573989775 624 L 624
Cdd:cd17645 439 L 439
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
232-624 |
1.31e-14 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 77.38 E-value: 1.31e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 232 YTSGTTNRPKGVLL----PEPVITAQCRSLLQaweYSPADRLLHVLPLHHIHGTINAVLTPLFAGSS-VEFMFPFNADAV 306
Cdd:PRK06060 152 YTSGTTGPPKAAIHrhadPLTFVDAMCRKALR---LTPEDTGLCSARMYFAYGLGNSVWFPLATGGSaVINSAPVTPEAA 228
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 307 wKRFAAPFLPtngtattappiSFFTVVPTVYSRLLAShktlspqmqaatSEATSPANLRVNISGSAALPTPVKAAWAALS 386
Cdd:PRK06060 229 -AILSARFGP-----------SVLYGVPNFFARVIDS------------CSPDSFRSLRCVVSAGEALELGLAERLMEFF 284
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 387 GGNVLLERYGMTEVGMALscglaFADRVD----ASVGWPLPSVEARLVDMDtGDVIEPGHElddhgrervGEIQLRGPTV 462
Cdd:PRK06060 285 GGIPILDGIGSTEVGQTF-----VSNRVDewrlGTLGRVLPPYEIRVVAPD-GTTAGPGVE---------GDLWVRGPAI 349
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 463 FREYWRNPDAtakeFVDDGGdgrgrWFKTGD-VAIrrepvgaagrnshrqpwaQGPMYFIQGRKTADIIKTGGEKVSALE 541
Cdd:PRK06060 350 AKGYWNRPDS----PVANEG-----WLDTRDrVCI------------------DSDGWVTYRCRADDTEVIGGVNVDPRE 402
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 542 VERELLSLPQVAEAAVVAVPSGNWGQKVGAVIIldPDHAVKWTPLEMR---RALKSRLAGYKIPQVMKIVNHIPRNAMGK 618
Cdd:PRK06060 403 VERLIIEDEAVAEAAVVAVRESTGASTLQAFLV--ATSGATIDGSVMRdlhRGLLNRLSAFKVPHRFAVVDRLPRTPNGK 480
|
....*.
gi 573989775 619 INKKQL 624
Cdd:PRK06060 481 LVRGAL 486
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
87-628 |
1.59e-14 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 76.36 E-value: 1.59e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 87 RRFTYGELLGDVARARERLREavdgKPLTGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLSPPFPAPELQY 166
Cdd:PRK07638 25 RVLTYKDWFESVCKVANWLNE----KESKNKTIAILLENRIEFLQ---------LFAGAAMAGWTCVPLDIKWKQDELKE 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 167 ILDQSAASVLVASPKFAAK----------AREVLATDLQQKPVYFELpkhtgsshgakeaVSLADEDFsgagLMLYTSGT 236
Cdd:PRK07638 92 RLAISNADMIVTERYKLNDlpdeegrvieIDEWKRMIEKYLPTYAPI-------------ENVQNAPF----YMGFTSGS 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 237 TNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTpLFAGSSVEFMFPFNADAVWKRfaapfLP 316
Cdd:PRK07638 155 TGKPKAFLRAQQSWLHSFDCNVHDFHMKREDSVLIAGTLVHSLFLYGAIST-LYVGQTVHLMRKFIPNQVLDK-----LE 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 317 TNGtattappISFFTVVPTVYSRLLASHKTLSPQMQAATSEATSPANLRVNIsgsaalptpvKAAWAALSggnvLLERYG 396
Cdd:PRK07638 229 TEN-------ISVMYTVPTMLESLYKENRVIENKMKIISSGAKWEAEAKEKI----------KNIFPYAK----LYEFYG 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 397 MTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDMDtGDVIEPGHElddhgrervGEIQLRGPTVFREYwRNPDATAKE 476
Cdd:PRK07638 288 ASELSFVTALVDEESERRPNSVGRPFHNVQVRICNEA-GEEVQKGEI---------GTVYVKSPQFFMGY-IIGGVLARE 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 477 FVDDGgdgrgrWFKTGDVAIRREpvgaagrnshrqpwaQGPMYfIQGRKTADIIkTGGEKVSALEVERELLSLPQVAEAA 556
Cdd:PRK07638 357 LNADG------WMTVRDVGYEDE---------------EGFIY-IVGREKNMIL-FGGINIFPEEIESVLHEHPAVDEIV 413
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 573989775 557 VVAVPSGNWGQKvgAVIILDPDHAVKwtplEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLVKAI 628
Cdd:PRK07638 414 VIGVPDSYWGEK--PVAIIKGSATKQ----QLKSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSWI 479
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
207-495 |
1.65e-14 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 76.94 E-value: 1.65e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 207 TGSSHGAKEAVSLADEDFSGAgLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAW-----EYSPADRLLHVLPLHHI-HG 280
Cdd:PTZ00216 247 KGHSAGSHHPLNIPENNDDLA-LIMYTSGTTGDPKGVMHTHGSLTAGILALEDRLndligPPEEDETYCSYLPLAHImEF 325
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 281 TINAVLtpLFAGSSVEFMFPFN-ADAvwkrFAAPflptNGTATTAPPiSFFTVVPTVYSRL-------LASHKTLSPQ-- 350
Cdd:PTZ00216 326 GVTNIF--LARGALIGFGSPRTlTDT----FARP----HGDLTEFRP-VFLIGVPRIFDTIkkaveakLPPVGSLKRRvf 394
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 351 -------MQAATSEATSPA-NLRVNISGSAALPTPVKAAwaaLSGG-----------NVLLER----YGMTE---VGMAL 404
Cdd:PTZ00216 395 dhayqsrLRALKEGKDTPYwNEKVFSAPRAVLGGRVRAM---LSGGgplsaatqefvNVVFGMviqgWGLTEtvcCGGIQ 471
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 405 SCGlafaDRVDASVGWPLPSVEARLVDMDtgdviEPGHELDDHGRervGEIQLRGPTVFREYWRNPDATaKEFVDDGGdg 484
Cdd:PTZ00216 472 RTG----DLEPNAVGQLLKGVEMKLLDTE-----EYKHTDTPEPR---GEILLRGPFLFKGYYKQEELT-REVLDEDG-- 536
|
330
....*....|.
gi 573989775 485 rgrWFKTGDVA 495
Cdd:PTZ00216 537 ---WFHTGDVG 544
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
115-493 |
1.13e-13 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 74.21 E-value: 1.13e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 115 TGERVAFLIENSYDYVVPeahsrdvvmFLAVMAARAIAVPLSPPFPAPELQYI----LDQSAASVLVASPKFAAKAREVL 190
Cdd:PRK05850 58 TGDRAVILAPQGLEYIVA---------FLGALQAGLIAVPLSVPQGGAHDERVsavlRDTSPSVVLTTSAVVDDVTEYVA 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 191 ATDLQQKPVYFELPK-HTGSSHGakeaVSLADEDFSGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAW-----EYS 264
Cdd:PRK05850 129 PQPGQSAPPVIEVDLlDLDSPRG----SDARPRDLPSTAYLQYTSGSTRTPAGVMVSHRNVIANFEQLMSDYfgdtgGVP 204
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 265 PADR-LLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPF---NADAVWKRFAApflpTNGTATTAPPISFFTvvptvysrl 340
Cdd:PRK05850 205 PPDTtVVSWLPFYHDMGLVLGVCAPILGGCPAVLTSPVaflQRPARWMQLLA----SNPHAFSAAPNFAFE--------- 271
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 341 LASHKTL------------------SPQMQAAT----SEATSPANLR----------------VNISGSAALPTPVKAAW 382
Cdd:PRK05850 272 LAVRKTSdddmagldlggvlgiisgSERVHPATlkrfADRFAPFNLRetairpsyglaeatvyVATREPGQPPESVRFDY 351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 383 AALSGGNVllERYGmTEVGMAL-SCGlafadrVDASvgwplPSVeaRLVDMDTGDVIEPGhelddhgreRVGEIQLRGPT 461
Cdd:PRK05850 352 EKLSAGHA--KRCE-TGGGTPLvSYG------SPRS-----PTV--RIVDPDTCIECPAG---------TVGEIWVHGDN 406
|
410 420 430
....*....|....*....|....*....|....*..
gi 573989775 462 VFREYWRNPDATAKEF----VD-DGGDGRGRWFKTGD 493
Cdd:PRK05850 407 VAAGYWQKPEETERTFgatlVDpSPGTPEGPWLRTGD 443
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
227-494 |
1.14e-13 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 74.36 E-value: 1.14e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 227 AGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVeFMFPfnadav 306
Cdd:PRK08043 367 AALILFTSGSEGHPKGVVHSHKSLLANVEQIKTIADFTPNDRFMSALPLFHSFGLTVGLFTPLLTGAEV-FLYP------ 439
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 307 wkrfaapflptngtattaPPISFFTVVPTVYSR----LLASHKTLSPQMQAAtseatSP---ANLRVNISGSAALPTPVK 379
Cdd:PRK08043 440 ------------------SPLHYRIVPELVYDRnctvLFGTSTFLGNYARFA-----NPydfARLRYVVAGAEKLQESTK 496
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 380 AAWAALSGGNVLlERYGMTEVGMALSCGLAFADRVdASVGWPLPSVEARLVDMdtgdviePGHElddHGrervGEIQLRG 459
Cdd:PRK08043 497 QLWQDKFGLRIL-EGYGVTECAPVVSINVPMAAKP-GTVGRILPGMDARLLSV-------PGIE---QG----GRLQLKG 560
|
250 260 270
....*....|....*....|....*....|....*...
gi 573989775 460 PTVFREYWR--NPDATAKEFVDDG-GDGRGRWFKTGDV 494
Cdd:PRK08043 561 PNIMNGYLRveKPGVLEVPTAENArGEMERGWYDTGDI 598
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
225-626 |
1.55e-13 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 72.39 E-value: 1.55e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 225 SGAGLMLYTSGTTNRPKGVLLpepviTAqcrSLLQAWEYSPADRL------LHVLPLHHIHGtINAVLTPLFAGSSvefm 298
Cdd:PRK07824 35 DDVALVVATSGTTGTPKGAML-----TA---AALTASADATHDRLggpgqwLLALPAHHIAG-LQVLVRSVIAGSE---- 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 299 fPFNADaVWKRFAAPFLPtNGTATTAPPISFFTVVPTVYSRLLAShktlspqMQAATSEATSPANLrvniSGSAALPTPV 378
Cdd:PRK07824 102 -PVELD-VSAGFDPTALP-RAVAELGGGRRYTSLVPMQLAKALDD-------PAATAALAELDAVL----VGGGPAPAPV 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 379 KAAWAALsGGNVLlERYGMTEvgmalSCGLAFADrvdasvGWPLPSVEARLVDmdtgdviepghelddhgrervGEIQLR 458
Cdd:PRK07824 168 LDAAAAA-GINVV-RTYGMSE-----TSGGCVYD------GVPLDGVRVRVED---------------------GRIALG 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 459 GPTVFREYwRNPDaTAKEFVDDGgdgrgrWFKTGDVAIRREPVgaagrnshrqpwaqgpmYFIQGRkTADIIKTGGEKVS 538
Cdd:PRK07824 214 GPTLAKGY-RNPV-DPDPFAEPG------WFRTDDLGALDDGV-----------------LTVLGR-ADDAISTGGLTVL 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 539 ALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGK 618
Cdd:PRK07824 268 PQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAVVGDGGPAP--TLEALRAHVARTLDRTAAPRELHVVDELPRRGIGK 345
|
....*...
gi 573989775 619 INKKQLVK 626
Cdd:PRK07824 346 VDRRALVR 353
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
221-624 |
1.62e-13 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 73.24 E-value: 1.62e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 221 DEDFSGaglMLYTSGTTNRPKGVllpepvITAQCRSLLQAWEYS------PADRLLHVLPLHHIHGTINAVLTPLFAGSS 294
Cdd:cd05937 86 PDDPAI---LIYTSGTTGLPKAA------AISWRRTLVTSNLLShdlnlkNGDRTYTCMPLYHGTAAFLGACNCLMSGGT 156
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 295 VEFMFPFNADAVWKRFAApflptnGTATtappisFFTVVPTVYSRLLASHKtlSPQMQAATSEATSPANLRVNIsgsaal 374
Cdd:cd05937 157 LALSRKFSASQFWKDVRD------SGAT------IIQYVGELCRYLLSTPP--SPYDRDHKVRVAWGNGLRPDI------ 216
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 375 ptpvkaaWAALSggnvllERYGMTEVG--MALSCGLAFADRVDA------SVG-------WPLPSVEARL-VDMDTGDVI 438
Cdd:cd05937 217 -------WERFR------ERFNVPEIGefYAATEGVFALTNHNVgdfgagAIGhhglirrWKFENQVVLVkMDPETDDPI 283
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 439 EPghelDDHG------RERVGEIQLRGP----TVFREYWRNPDATAKEFVDD---GGDGrgrWFKTGDvAIRREpvgaag 505
Cdd:cd05937 284 RD----PKTGfcvrapVGEPGEMLGRVPfknrEAFQGYLHNEDATESKLVRDvfrKGDI---YFRTGD-LLRQD------ 349
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 506 rnshrqpwAQGPMYFIQgrKTADIIKTGGEKVSALEVERELLSLPQVAEAAV--VAVPsGNWGQKVGAVIILDPDHAVK- 582
Cdd:cd05937 350 --------ADGRWYFLD--RLGDTFRWKSENVSTTEVADVLGAHPDIAEANVygVKVP-GHDGRAGCAAITLEESSAVPt 418
|
410 420 430 440
....*....|....*....|....*....|....*....|...
gi 573989775 583 -WTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:cd05937 419 eFTKSLLASLARKNLPSYAVPLFLRLTEEVATTDNHKQQKGVL 461
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
67-619 |
2.46e-13 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 72.99 E-value: 2.46e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 67 FEAVAAH---DPTSTAVVH----ALSGRRFTYGELLGDVARARERLReavDGKPLTGERVAFliensYDYVVPEAhsrdV 139
Cdd:cd17634 56 ANALDRHlreNGDRTAIIYegddTSQSRTISYRELHREVCRFAGTLL---DLGVKKGDRVAI-----YMPMIPEA----A 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 140 VMFLAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREV-------LATDLQQKPVYFEL-PKHTGSSH 211
Cdd:cd17634 124 VAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLLITADGGVRAGRSVplkknvdDALNPNVTSVEHVIvLKRTGSDI 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 212 GAKEAVSLADEDFSGAG---------------LMLYTSGTTNRPKGVLLPE---PVITAQcrSLLQAWEYSPADRLLHVL 273
Cdd:cd17634 204 DWQEGRDLWWRDLIAKAspehqpeamnaedplFILYTSGTTGKPKGVLHTTggyLVYAAT--TMKYVFDYGPGDIYWCTA 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 274 PLHHIHGTINAVLTPLFAGSSVeFMFPfnadavwkrfAAPFLPTngtattapPISFFTVVptvySRLLASHKTLSPQMQA 353
Cdd:cd17634 282 DVGWVTGHSYLLYGPLACGATT-LLYE----------GVPNWPT--------PARMWQVV----DKHGVNILYTAPTAIR 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 354 ATSEATSPANLRVNISGSAAL--------PTPVKAAWAALSGGNV-LLERYGMTEVGMALSCGLAFADRVDA-SVGWPLP 423
Cdd:cd17634 339 ALMAAGDDAIEGTDRSSLRILgsvgepinPEAYEWYWKKIGKEKCpVVDTWWQTETGGFMITPLPGAIELKAgSATRPVF 418
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 424 SVEARLVDmDTGDVIEPGHElddhgrervGEIQLRG--PTVFREYWRNPDATAKEFVddgGDGRGRWFkTGDVAIRREpv 501
Cdd:cd17634 419 GVQPAVVD-NEGHPQPGGTE---------GNLVITDpwPGQTRTLFGDHERFEQTYF---STFKGMYF-SGDGARRDE-- 482
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 502 gaagrnshrqpwaQGpMYFIQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILdpDHAV 581
Cdd:cd17634 483 -------------DG-YYWITGR-SDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVL--NHGV 545
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 573989775 582 KWTPL---EMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKI 619
Cdd:cd17634 546 EPSPElyaELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKI 586
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
63-636 |
4.49e-13 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 72.14 E-value: 4.49e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 63 RLPIFEAVAAHDPTSTAVVHAL-------SGRRFTYGELLGDVARARERLREAVDGKpltGERVAFLIEnsydyVVPEAh 135
Cdd:cd05968 59 RMNIVEQLLDKWLADTRTRPALrwegedgTSRTLTYGELLYEVKRLANGLRALGVGK---GDRVGIYLP-----MIPEI- 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 136 srdVVMFLAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVY--------------- 200
Cdd:cd05968 130 ---VPAFLAVARIGGIVVPIFSGFGKEAAATRLQDAEAKALITADGFTRRGREVNLKEEADKACAqcptvekvvvvrhlg 206
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 201 FELPKHTGSSHGAKEAVSLADEDFSGAG----LML-YTSGTTNRPKGVLLPE---PVITAQcrSLLQAWEYSPADRLLHV 272
Cdd:cd05968 207 NDFTPAKGRDLSYDEEKETAGDGAERTEsedpLMIiYTSGTTGKPKGTVHVHagfPLKAAQ--DMYFQFDLKPGDLLTWF 284
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 273 LPLHHIHGTInAVLTPLFAGSSVeFM------FPfNADAVWkRFAAPFLPTNgtattappisfFTVVPTVYsRLLASHKT 346
Cdd:cd05968 285 TDLGWMMGPW-LIFGGLILGATM-VLydgapdHP-KADRLW-RMVEDHEITH-----------LGLSPTLI-RALKPRGD 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 347 lspqmqaATSEATSPANLRVNIS-GSAALPTPVKAAWAALSGGNVLLERY-GMTEVGMALSCGLAFADRVDASVGWPLPS 424
Cdd:cd05968 349 -------APVNAHDLSSLRVLGStGEPWNPEPWNWLFETVGKGRNPIINYsGGTEISGGILGNVLIKPIKPSSFNGPVPG 421
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 425 VEARLVDmDTGDVIepghelddhgRERVGEIQLRGPTV--FREYWRNPDATAKEFVDdggDGRGRWFKtGDVAIRREpvg 502
Cdd:cd05968 422 MKADVLD-ESGKPA----------RPEVGELVLLAPWPgmTRGFWRDEDRYLETYWS---RFDNVWVH-GDFAYYDE--- 483
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 503 aagrnshrqpwaqGPMYFIQGRkTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDhaVK 582
Cdd:cd05968 484 -------------EGYFYILGR-SDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFVVLKPG--VT 547
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*..
gi 573989775 583 WTPL---EMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKqLVKAIFVEETSGD 636
Cdd:cd05968 548 PTEAlaeELMERVADELGKPLSPERILFVKDLPKTRNAKVMRR-VIRAAYLGKELGD 603
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
66-624 |
6.79e-13 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 72.51 E-value: 6.79e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKPLtgeRVAFLIENSYDYVVPeahsrdvvmFLAV 145
Cdd:PRK05691 2193 LFAAQAARTPQAPALTFA--GQTLSYAELDARANRLARALRERGVGPQV---RVGLALERSLEMVVG---------LLAI 2258
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 146 MAARAIAVPLSPPFPAPELQYILDQSAASVLVASpkfaakaREVLATdlqqkpvYFELPKHTGSSHGAKEAVSLADedFS 225
Cdd:PRK05691 2259 LKAGGAYVPLDPEYPLERLHYMIEDSGIGLLLSD-------RALFEA-------LGELPAGVARWCLEDDAAALAA--YS 2322
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 226 GAGL-----------MLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTiNAVLTPLFAGSS 294
Cdd:PRK05691 2323 DAPLpflslpqhqayLIYTSGSTGKPKGVVVSHGEIAMHCQAVIERFGMRADDCELHFYSINFDAAS-ERLLVPLLCGAR 2401
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 295 VefmfPFNADAVWKRFAAPFLptngtaTTAPPISFFTVVPTvYSRLLASHktlspqmqAATSEATSPanLRVNISGSAAL 374
Cdd:PRK05691 2402 V----VLRAQGQWGAEEICQL------IREQQVSILGFTPS-YGSQLAQW--------LAGQGEQLP--VRMCITGGEAL 2460
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 375 P----TPVKAAWAAlsggNVLLERYGMTE-VGMALSCgLAfADRVDASVG-WPLPSV----EARLVDMDTGDVIEPGhel 444
Cdd:PRK05691 2461 TgehlQRIRQAFAP----QLFFNAYGPTEtVVMPLAC-LA-PEQLEEGAAsVPIGRVvgarVAYILDADLALVPQGA--- 2531
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 445 ddhgrerVGEIQLRGPTVFREYWRNPDATAKEFVDD--GGDGrGRWFKTGDVAIRREP--VGAAGRNSHRqpwaqgpmyf 520
Cdd:PRK05691 2532 -------TGELYVGGAGLAQGYHDRPGLTAERFVADpfAADG-GRLYRTGDLVRLRADglVEYVGRIDHQ---------- 2593
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 521 iqgrktadiIKTGGEKVSALEVERELLSLPQVAEAAVVA--VPSGNwgQKVGAVI--ILDPDHAVKWTPLEMRRA-LKSR 595
Cdd:PRK05691 2594 ---------VKIRGFRIELGEIESRLLEHPAVREAVVLAldTPSGK--QLAGYLVsaVAGQDDEAQAALREALKAhLKQQ 2662
|
570 580
....*....|....*....|....*....
gi 573989775 596 LAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK05691 2663 LPDYMVPAHLILLDSLPLTANGKLDRRAL 2691
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
217-545 |
9.49e-13 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 70.57 E-value: 9.49e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 217 VSLADEDfsgaGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHhihgtinAVLTPLFAGSSVe 296
Cdd:cd05910 81 IPKADEP----AAILFTSGSTGTPKGVVYRHGTFAAQIDALRQLYGIRPGEVDLATFPLF-------ALFGPALGLTSV- 148
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 297 fmfpfnadavwkrfaAPFLPTNGTATTAPPISFFTVvptvySRLLASHKTLSPQMQAATSEA------TSPANLRVnISG 370
Cdd:cd05910 149 ---------------IPDMDPTRPARADPQKLVGAI-----RQYGVSIVFGSPALLERVARYcaqhgiTLPSLRRV-LSA 207
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 371 SAALPTPVKAAW-AALSGGNVLLERYGMTE---VGMALSCGLaFADRVDAS-------VGWPLPSVEARLVDMDTGDVIE 439
Cdd:cd05910 208 GAPVPIALAARLrKMLSDEAEILTPYGATEalpVSSIGSREL-LATTTAATsggagtcVGRPIPGVRVRIIEIDDEPIAE 286
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 440 PG--HELDDHGrerVGEIQLRGPTVFREYWRNPDATAKEFVDDGGDgrGRWFKTGDVAI--RREPVGAAGRNSHRQPWAQ 515
Cdd:cd05910 287 WDdtLELPRGE---IGEITVTGPTVTPTYVNRPVATALAKIDDNSE--GFWHRMGDLGYldDEGRLWFCGRKAHRVITTG 361
|
330 340 350 360
....*....|....*....|....*....|....*....|.
gi 573989775 516 GPMYFI----------QGRKTADI-IKTGGEKVSALEVERE 545
Cdd:cd05910 362 GTLYTEpvervfnthpGVRRSALVgVGKPGCQLPVLCVEPL 402
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
230-543 |
1.18e-12 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 70.85 E-value: 1.18e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 230 MLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHV----LPLHHIHGTINAVLTPLFAGSSVEFMFPfnaDA 305
Cdd:cd05933 155 LIYTSGTTGMPKGVMLSHDNITWTAKAASQHMDLRPATVGQESvvsyLPLSHIAAQILDIWLPIKVGGQVYFAQP---DA 231
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 306 VwkrfaapflptNGT-ATT---APPISFFTvVPTVYSRLL-------ASHKTLSPQMQAATSEATSPANLRVNISGSaal 374
Cdd:cd05933 232 L-----------KGTlVKTlreVRPTAFMG-VPRVWEKIQekmkavgAKSGTLKRKIASWAKGVGLETNLKLMGGES--- 296
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 375 PTPVKAAWAA-------------------LSGG--------------NV-LLERYGMTEvgmalSCGLAFADRVDA---- 416
Cdd:cd05933 297 PSPLFYRLAKklvfkkvrkalgldrcqkfFTGAapisretlefflslNIpIMELYGMSE-----TSGPHTISNPQAyrll 371
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 417 SVGWPLPSVEARLvdmdtgdviepgHELDDHGrerVGEIQLRGPTVFREYWRNPDATaKEFVDDGGdgrgrWFKTGDVAi 496
Cdd:cd05933 372 SCGKALPGCKTKI------------HNPDADG---IGEICFWGRHVFMGYLNMEDKT-EEAIDEDG-----WLHSGDLG- 429
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 573989775 497 RREPVGaagrnshrqpwaqgpMYFIQGRKTADIIKTGGEKVSALEVE 543
Cdd:cd05933 430 KLDEDG---------------FLYITGRIKELIITAGGENVPPVPIE 461
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
230-495 |
1.68e-12 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 70.43 E-value: 1.68e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 230 MLYTSGTTNRPKGVLLPE----PVITAQCRSLLQAWE-YSPADRLLHVLPLHHIhgtINAVLTPLF--AGSSVEFmfpfn 302
Cdd:PLN02614 228 IMYTSGTTGDPKGVMISNesivTLIAGVIRLLKSANAaLTVKDVYLSYLPLAHI---FDRVIEECFiqHGAAIGF----- 299
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 303 adavWKRFAApfLPTNGTATTAPPIsfFTVVPTVYSRLLAS-HKTLSP------------------QMQAATSE-ATSP- 361
Cdd:PLN02614 300 ----WRGDVK--LLIEDLGELKPTI--FCAVPRVLDRVYSGlQKKLSDggflkkfvfdsafsykfgNMKKGQSHvEASPl 371
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 362 --------------ANLRVNISGSAALPTPVKAAWAALSGGNVLlERYGMTEvgmalSCGLAFA---DRVD--ASVGWPL 422
Cdd:PLN02614 372 cdklvfnkvkqglgGNVRIILSGAAPLASHVESFLRVVACCHVL-QGYGLTE-----SCAGTFVslpDELDmlGTVGPPV 445
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 573989775 423 PSVEARLVDMdtgdviePGHELDDHGRERVGEIQLRGPTVFREYWRNPDATAKEFVDDggdgrgrWFKTGDVA 495
Cdd:PLN02614 446 PNVDIRLESV-------PEMEYDALASTPRGEICIRGKTLFSGYYKREDLTKEVLIDG-------WLHTGDVG 504
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
75-624 |
2.94e-12 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 69.43 E-value: 2.94e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHalSGRRFTYGELlgdvARARERLREAVDGKPLTGER-VAFLIENSYDYVVPeahsrdvvmFLAVMAARAIAV 153
Cdd:cd17656 2 PDAVAVVF--ENQKLTYREL----NERSNQLARFLREKGVKKDSiVAIMMERSAEMIVG---------ILGILKAGGAFV 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 154 PLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLATDLQQKPVYfelpkhtgsSHGAKEAVSL--ADEDFSgagLML 231
Cdd:cd17656 67 PIDPEYPEERRIYIMLDSGVRVVLTQRHLKSKLSFNKSTILLEDPSI---------SQEDTSNIDYinNSDDLL---YII 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 232 YTSGTTNRPKGVLLPEPVITaqcrSLLQ-AWEYSPADRLLHVLPL---------HHIHGTInavltpLFAGSsvefMFPF 301
Cdd:cd17656 135 YTSGTTGKPKGVQLEHKNMV----NLLHfEREKTNINFSDKVLQFatcsfdvcyQEIFSTL------LSGGT----LYII 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 302 NADA---VWKRFAapFLPTNGTATTAPPISFFtvvptvysRLLASHKTLSPQMqaatseatsPANLRVNISGSAALPTPV 378
Cdd:cd17656 201 REETkrdVEQLFD--LVKRHNIEVVFLPVAFL--------KFIFSEREFINRF---------PTCVKHIITAGEQLVITN 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 379 KAAWAALSGGNVLLERYGMTEVGMALSCglafadRVDASVGWPL------PSVEARLVDMDTGDVIEPghelddhgRERV 452
Cdd:cd17656 262 EFKEMLHEHNVHLHNHYGPSETHVVTTY------TINPEAEIPElppigkPISNTWIYILDQEQQLQP--------QGIV 327
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 453 GEIQLRGPTVFREYWRNPDATAKEFVDDGGDGRGRWFKTGDVAiRREPVGA---AGRNSHRqpwaqgpmyfiqgrktadi 529
Cdd:cd17656 328 GELYISGASVARGYLNRQELTAEKFFPDPFDPNERMYRTGDLA-RYLPDGNiefLGRADHQ------------------- 387
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 530 IKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDpdhaVKWTPLEMRRALKSRLAGYKIPQVMKIVN 609
Cdd:cd17656 388 VKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFVME----QELNISQLREYLAKQLPEYMIPSFFVPLD 463
|
570
....*....|....*
gi 573989775 610 HIPRNAMGKINKKQL 624
Cdd:cd17656 464 QLPLTPNGKVDRKAL 478
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
229-494 |
5.97e-12 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 68.48 E-value: 5.97e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 LMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPA-DRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFP--FNAD- 304
Cdd:PRK07768 156 LMQLTSGSTGSPKAVQITHGNLYANAEAMFVAAEFDVEtDVMVSWLPLFHDMGMVGFLTVPMYFGAELVKVTPmdFLRDp 235
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 305 AVWKRFAAPFlptNGTATTAPPISFftvvpTVYSRLLAShktlspqmqAATSEATSPANLRVNISGsaALPTPVKAAWAA 384
Cdd:PRK07768 236 LLWAELISKY---RGTMTAAPNFAY-----ALLARRLRR---------QAKPGAFDLSSLRFALNG--AEPIDPADVEDL 296
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 385 LSGG-------NVLLERYGMTEVGMALS-----CGLAFaDRVDA---------------------SVGWPLPSVEARLVD 431
Cdd:PRK07768 297 LDAGarfglrpEAILPAYGMAEATLAVSfspcgAGLVV-DEVDAdllaalrravpatkgntrrlaTLGPPLPGLEVRVVD 375
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 573989775 432 MDtGDVIEPghelddhgrERVGEIQLRGPTVFREYwRNPDAtAKEFVDDGGdgrgrWFKTGDV 494
Cdd:PRK07768 376 ED-GQVLPP---------RGVGVIELRGESVTPGY-LTMDG-FIPAQDADG-----WLDTGDL 421
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
71-497 |
8.28e-12 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 67.98 E-value: 8.28e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 71 AAHDPTSTAVVHALSG---RRFTYGELLgdvARAReRLREAVDGKPLTGER-VAFLIENSYDyvvpeaHSrdvVMFLAVM 146
Cdd:PRK08180 49 AQEAPDRVFLAERGADggwRRLTYAEAL---ERVR-AIAQALLDRGLSAERpLMILSGNSIE------HA---LLALAAM 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 147 AARAIAVPLSPPFPAP-----ELQYILDQSAASVLVASPKfAAKAREVLATDLQQKPVYFelpkhTGSSHGAKEAVSLAD 221
Cdd:PRK08180 116 YAGVPYAPVSPAYSLVsqdfgKLRHVLELLTPGLVFADDG-AAFARALAAVVPADVEVVA-----VRGAVPGRAATPFAA 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 222 --EDFSGAGLM--------------LYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYsPADR---LLHVLPLHHIHGTI 282
Cdd:PRK08180 190 llATPPTAAVDaahaavgpdtiakfLFTSGSTGLPKAVINTHRMLCANQQMLAQTFPF-LAEEppvLVDWLPWNHTFGGN 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 283 NAVLTPLFAGSSvefMFPFNADAVWKRFAApflptngtattappisffT------VVPTVYSRLLASHKTLSPQMQAATS 356
Cdd:PRK08180 269 HNLGIVLYNGGT---LYIDDGKPTPGGFDE------------------TlrnlreISPTVYFNVPKGWEMLVPALERDAA 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 357 EATSP-ANLRVNISGSAALPTPVkaaWAAL--SGGNVLLER------YGMTEVG-MALSCglafADRVDAS--VGWPLPS 424
Cdd:PRK08180 328 LRRRFfSRLKLLFYAGAALSQDV---WDRLdrVAEATCGERirmmtgLGMTETApSATFT----TGPLSRAgnIGLPAPG 400
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 573989775 425 VEARLVDMDtgdviepghelddhGRErvgEIQLRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGDvAIR 497
Cdd:PRK08180 401 CEVKLVPVG--------------GKL---EVRVKGPNVTPGYWRAPELTAEAFDEEG------YYRSGD-AVR 449
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
75-624 |
1.27e-11 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 67.04 E-value: 1.27e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 75 PTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKPltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVP 154
Cdd:cd17648 1 PDRVAVVYG--DKRLTYRELNERANRLAHYLLSVAEIRP--DDLVGLVLDKSELMII---------AILAVWKAGAAYVP 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 155 LSPPFPAPELQYILDQSAASVLVASPKFAAKArevlatdlqqkpvyfelpkhtgsshgakeavsladedfsgaglmLYTS 234
Cdd:cd17648 68 IDPSYPDERIQFILEDTGARVVITNSTDLAYA--------------------------------------------IYTS 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 235 GTTNRPKGVLLPEPVITAQCRSLLQawEYSPADRLLHVLPLHHihgtiNAVLTPlfagsSVEFMfpfnADAVwkrfaapf 314
Cdd:cd17648 104 GTTGKPKGVLVEHGSVVNLRTSLSE--RYFGRDNGDEAVLFFS-----NYVFDF-----FVEQM----TLAL-------- 159
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 315 lpTNGTATTAPPISFFTVVPTVY-----SRLLASHKTLSPQMQAATSEATSpanLRVNISGSAALPTPVKAAWAALSGGN 389
Cdd:cd17648 160 --LNGQKLVVPPDEMRFDPDRFYayinrEKVTYLSGTPSVLQQYDLARLPH---LKRVDAAGEEFTAPVFEKLRSRFAGL 234
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 390 VLLErYGMTEVGM-ALSCGLAFADRVDASVGWPLPSVEARLVDMDTGDVIEPGhelddhgrerVGEIQLRGPTVFREYWR 468
Cdd:cd17648 235 IINA-YGPTETTVtNHKRFFPGDQRFDKSLGRPVRNTKCYVLNDAMKRVPVGA----------VGELYLGGDGVARGYLN 303
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 469 NPDATAKEFV-------DDGGDGR-GRWFKTGDVAiRREPvgaagrnshrqpwaQGPMYFIqGRKTADiIKTGGEKVSAL 540
Cdd:cd17648 304 RPELTAERFLpnpfqteQERARGRnARLYKTGDLV-RWLP--------------SGELEYL-GRNDFQ-VKIRGQRIEPG 366
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 541 EVERELLSLP----QVAEAAVVAVPSGNWGQK--VGaVIILDPDHAvkwTPLEMRRALKSRLAGYKIPQVMKIVNHIPRN 614
Cdd:cd17648 367 EVEAALASYPgvreCAVVAKEDASQAQSRIQKylVG-YYLPEPGHV---PESDLLSFLRAKLPRYMVPARLVRLEGIPVT 442
|
570
....*....|
gi 573989775 615 AMGKINKKQL 624
Cdd:cd17648 443 INGKLDVRAL 452
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
209-545 |
2.86e-11 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 66.46 E-value: 2.86e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 209 SSHGAKEAVSLADEDFSGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGtinavltP 288
Cdd:PRK09274 158 LRDGAAAPFPMADLAPDDMAAILFTSGSTGTPKGVVYTHGMFEAQIEALREDYGIEPGEIDLPTFPLFALFG-------P 230
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 289 LFAGSSV----EFMFPFNADAVwKRFAApfLPTNGtattappISFFTVVPTVYSRLLashktlspqmQAATSEATSPANL 364
Cdd:PRK09274 231 ALGMTSVipdmDPTRPATVDPA-KLFAA--IERYG-------VTNLFGSPALLERLG----------RYGEANGIKLPSL 290
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 365 RVNISGSAALPTPVKAAWAA-LSGGNVLLERYGMTE------VGM--ALSCGLAFADRVD-ASVGWPLPSVEARLVDMDT 434
Cdd:PRK09274 291 RRVISAGAPVPIAVIERFRAmLPPDAEILTPYGATEalpissIESreILFATRAATDNGAgICVGRPVDGVEVRIIAISD 370
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 435 GdviePGHELDDHGR---ERVGEIQLRGPTVFREYWRNPDATAKEFVDDGgdGRGRWFKTGDVAiRREPVGA---AGRNS 508
Cdd:PRK09274 371 A----PIPEWDDALRlatGEIGEIVVAGPMVTRSYYNRPEATRLAKIPDG--QGDVWHRMGDLG-YLDAQGRlwfCGRKA 443
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 573989775 509 HRQPWAQGPMYFIQG----------RKTADI-IKTGGEKVSALEVERE 545
Cdd:PRK09274 444 HRVETAGGTLYTIPCerifnthpgvKRSALVgVGVPGAQRPVLCVELE 491
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
66-620 |
6.03e-11 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 65.76 E-value: 6.03e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEAV--AA--HDPTSTAVVHALSGRrFTYGELLgdvararerLREAVDGKPLT-----GERVAFLIENSydyvvpeahS 136
Cdd:PRK06814 633 LFEALieAAkiHGFKKLAVEDPVNGP-LTYRKLL---------TGAFVLGRKLKkntppGENVGVMLPNA---------N 693
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 137 RDVVMFLAVMAARAIAVPLSppFPAPELQYILDQSAASV--LVASPKFAAKAR---EVLATDLQQKPVYFELPKHT---- 207
Cdd:PRK06814 694 GAAVTFFALQSAGRVPAMIN--FSAGIANILSACKAAQVktVLTSRAFIEKARlgpLIEALEFGIRIIYLEDVRAQigla 771
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 208 ----GSSHGAKEAVSLADEDFSGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTIN 283
Cdd:PRK06814 772 dkikGLLAGRFPLVYFCNRDPDDPAVILFTSGSEGTPKGVVLSHRNLLANRAQVAARIDFSPEDKVFNALPVFHSFGLTG 851
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 284 AVLTPLFAGSSVeFMFP--------------------FNADAvwkrfaapFLptNGTATTAPPISFFTvvptvysrllas 343
Cdd:PRK06814 852 GLVLPLLSGVKV-FLYPsplhyriipeliydtnatilFGTDT--------FL--NGYARYAHPYDFRS------------ 908
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 344 hktlspqmqaatseatspanLRVNISGSAALPTPVKAAWAALSGGNVlLERYGMTEVGMALSCGLAFADRVdASVGWPLP 423
Cdd:PRK06814 909 --------------------LRYVFAGAEKVKEETRQTWMEKFGIRI-LEGYGVTETAPVIALNTPMHNKA-GTVGRLLP 966
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 424 SVEARLvdmdtgdviEPGHELDDHGRERVgeiqlRGPTVFREYWRNPDATAKEFVDDGgdgrgrWFKTGD-VAIRrepvg 502
Cdd:PRK06814 967 GIEYRL---------EPVPGIDEGGRLFV-----RGPNVMLGYLRAENPGVLEPPADG------WYDTGDiVTID----- 1021
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 503 aagrnshrqpwAQGpMYFIQGRKTAdIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKvgavIILDPDHAvK 582
Cdd:PRK06814 1022 -----------EEG-FITIKGRAKR-FAKIAGEMISLAAVEELAAELWPDALHAAVSIPDARKGER----IILLTTAS-D 1083
|
570 580 590
....*....|....*....|....*....|....*....
gi 573989775 583 WTPLEMRRALKSRLAG-YKIPQVMKIVNHIPRNAMGKIN 620
Cdd:PRK06814 1084 ATRAAFLAHAKAAGASeLMVPAEIITIDEIPLLGTGKID 1122
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
87-495 |
6.51e-11 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 65.45 E-value: 6.51e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 87 RRFTYGELLgdvaRARERLREA-VDGKPLTGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLSPPFPA---- 161
Cdd:PRK12582 79 RKVTYGEAK----RAVDALAQAlLDLGLDPGRPVMILSGNSIEHAL---------MTLAAMQAGVPAAPVSPAYSLmshd 145
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 162 -PELQYILDQSAASVLVA--SPKFAAKAREVLATDLQQKPVYfELPKHTGSSHGAKEAVSLADEDFSGA---------GL 229
Cdd:PRK12582 146 hAKLKHLFDLVKPRVVFAqsGAPFARALAALDLLDVTVVHVT-GPGEGIASIAFADLAATPPTAAVAAAiaaitpdtvAK 224
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 230 MLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADR---LLHVLPLHHIHGTiNAVLTPLFAGSSVEFMfpfnaDAv 306
Cdd:PRK12582 225 YLFTSGSTGMPKAVINTQRMMCANIAMQEQLRPREPDPPppvSLDWMPWNHTMGG-NANFNGLLWGGGTLYI-----DD- 297
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 307 WKRFAAPFLPTNGTATTAPPISFFTVvPTVYSrLLASHKTLSPQMQAATSEatspaNLRVNISGSAALPTPVKAAWAALS 386
Cdd:PRK12582 298 GKPLPGMFEETIRNLREISPTVYGNV-PAGYA-MLAEAMEKDDALRRSFFK-----NLRLMAYGGATLSDDLYERMQALA 370
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 387 GGN-----VLLERYGMTEVGmALSCGLAFADRVDASVGWPLPSVEARLVDmdTGDVIEpghelddhgrervgeIQLRGPT 461
Cdd:PRK12582 371 VRTtghriPFYTGYGATETA-PTTTGTHWDTERVGLIGLPLPGVELKLAP--VGDKYE---------------VRVKGPN 432
|
410 420 430
....*....|....*....|....*....|....
gi 573989775 462 VFREYWRNPDATAKEFVDDGgdgrgrWFKTGDVA 495
Cdd:PRK12582 433 VTPGYHKDPELTAAAFDEEG------FYRLGDAA 460
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
116-547 |
7.26e-11 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 65.03 E-value: 7.26e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 116 GERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLspPFPAP---------ELQYILDQSAASVLVASPKFAAKA 186
Cdd:PRK09192 74 GDRVALIAETDGDFVE---------AFFACQYAGLVPVPL--PLPMGfggresyiaQLRGMLASAQPAAIITPDELLPWV 142
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 187 REvlATDLQQkpvyfelPKHTGSsHGAKEAVSLADEDFSGA-----GLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQ-A 260
Cdd:PRK09192 143 NE--ATHGNP-------LLHVLS-HAWFKALPEADVALPRPtpddiAYLQYSSGSTRFPRGVIITHRALMANLRAISHdG 212
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 261 WEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFmfpfnadavwkrfaapfLPTNGtattappisfFTVVPTVYSRL 340
Cdd:PRK09192 213 LKVRPGDRCVSWLPFYHDMGLVGFLLTPVATQLSVDY-----------------LPTRD----------FARRPLQWLDL 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 341 LASHK---TLSP---------QMQAATSEATSPANLRV-NISGSAALPTPVKAAWAALSG----GNVLLERYGMTEVGMA 403
Cdd:PRK09192 266 ISRNRgtiSYSPpfgyelcarRVNSKDLAELDLSCWRVaGIGADMIRPDVLHQFAEAFAPagfdDKAFMPSYGLAEATLA 345
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 404 LS-----CGLAFaDRVDASV-----------------------GWPLPSVEARLVDmdtgdviEPGHELDDHgreRVGEI 455
Cdd:PRK09192 346 VSfsplgSGIVV-EEVDRDRleyqgkavapgaetrrvrtfvncGKALPGHEIEIRN-------EAGMPLPER---VVGHI 414
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 456 QLRGPTVFREYWRNPDaTAKEFVDDGgdgrgrWFKTGDVAIRRE----PVGAA-------GRNSHRQ--PWA--QGPmyf 520
Cdd:PRK09192 415 CVRGPSLMSGYFRDEE-SQDVLAADG------WLDTGDLGYLLDgylyITGRAkdliiinGRNIWPQdiEWIaeQEP--- 484
|
490 500 510
....*....|....*....|....*....|....*....
gi 573989775 521 iqGRKTADII-----KTGGEKVSAL-------EVERELL 547
Cdd:PRK09192 485 --ELRSGDAAafsiaQENGEKIVLLvqcrisdEERRGQL 521
|
|
| PRK07769 |
PRK07769 |
long-chain-fatty-acid--CoA ligase; Validated |
116-493 |
1.30e-10 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 181109 [Multi-domain] Cd Length: 631 Bit Score: 64.37 E-value: 1.30e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 116 GERVAFLIENSYDYVVPeahsrdvvmFLAVMAARAIAVPL-SPPFP--APELQYILDQSAASVLVASPKFAAKAREVLAT 192
Cdd:PRK07769 79 GDRVAILAPQNLDYLIA---------FFGALYAGRIAVPLfDPAEPghVGRLHAVLDDCTPSAILTTTDSAEGVRKFFRA 149
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 193 -DLQQKPVYF---ELPKHTGSSHgakEAVSLADEDFSgagLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADR 268
Cdd:PRK07769 150 rPAKERPRVIavdAVPDEVGATW---VPPEANEDTIA---YLQYTSGSTRIPAGVQITHLNLPTNVLQVIDALEGQEGDR 223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 269 LLHVLPLHHIHGTINAVLTPLfAGSSVEFMFPfnADAV-----W-KRFAAPFLPTNGTATTAPPISFftvvptvysrLLA 342
Cdd:PRK07769 224 GVSWLPFFHDMGLITVLLPAL-LGHYITFMSP--AAFVrrpgrWiRELARKPGGTGGTFSAAPNFAF----------EHA 290
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 343 SHKTLSPqmqaatsEATSPANLRvNISGSAALPTPVKAawAALSGGNVLLERYGMTEVGMALSCGLAFA----------- 411
Cdd:PRK07769 291 AARGLPK-------DGEPPLDLS-NVKGLLNGSEPVSP--ASMRKFNEAFAPYGLPPTAIKPSYGMAEAtlfvsttpmde 360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 412 -------DR----------VDASVGWPLPSVEARLVDMDTGDVI---EPGHELDDhgrERVGEIQLRGPTVFREYWRNPD 471
Cdd:PRK07769 361 eptviyvDRdelnagrfveVPADAPNAVAQVSAGKVGVSEWAVIvdpETASELPD---GQIGEIWLHGNNIGTGYWGKPE 437
|
410 420 430
....*....|....*....|....*....|...
gi 573989775 472 ATAKEFVD-----------DGGDGRGRWFKTGD 493
Cdd:PRK07769 438 ETAATFQNilksrlseshaEGAPDDALWVRTGD 470
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
66-624 |
1.51e-10 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 64.80 E-value: 1.51e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 66 IFEA-VAAHdPTSTAVvhALSGRRFTYGELLGDVARARERLREAVDG--KPltgerVAFLIENSYDYVVpeahsrdvvMF 142
Cdd:PRK05691 3725 LFEAqVAAH-PQRIAA--SCLDQQWSYAELNRAANRLGHALRAAGVGvdQP-----VALLAERGLDLLG---------MI 3787
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 143 LAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVL-ATDLQQKP---VYFELPKHTGSSHGAKEavs 218
Cdd:PRK05691 3788 VGSFKAGAGYLPLDPGLPAQRLQRIIELSRTPVLVCSAACREQARALLdELGCANRPrllVWEEVQAGEVASHNPGI--- 3864
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 219 ladedFSGA---GLMLYTSGTTNRPKGVLLPEpvitaqcrsllqaweyspadrllhvlplhhiHGTINAVLtplfagSSV 295
Cdd:PRK05691 3865 -----YSGPdnlAYVIYTSGSTGLPKGVMVEQ-------------------------------RGMLNNQL------SKV 3902
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 296 EFMFPFNADA------------VWKRFAAPFL--------------PTNGTA-TTAPPISFFTVVPTVYSRLLAS-HKTL 347
Cdd:PRK05691 3903 PYLALSEADViaqtasqsfdisVWQFLAAPLFgarveivpnaiahdPQGLLAhVQAQGITVLESVPSLIQGMLAEdRQAL 3982
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 348 SpqmqaatseatspaNLRVNISGSAALPTPVKAAWAALSGGNVLLERYGMTEVgmalSCGLAFAdRVDAS--------VG 419
Cdd:PRK05691 3983 D--------------GLRWMLPTGEAMPPELARQWLQRYPQIGLVNAYGPAEC----SDDVAFF-RVDLAstrgsylpIG 4043
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 420 WPLPSVEARLVDmDTGDVIEPGhelddhgreRVGEIQLRGPTVFREYWRNPDATAKEFVDD--GGDGRgRWFKTGDVAiR 497
Cdd:PRK05691 4044 SPTDNNRLYLLD-EALELVPLG---------AVGELCVAGTGVGRGYVGDPLRTALAFVPHpfGAPGE-RLYRTGDLA-R 4111
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 498 REPVGA---AGRNSHRqpwaqgpmyfiqgrktadiIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVII 574
Cdd:PRK05691 4112 RRSDGVleyVGRIDHQ-------------------VKIRGYRIELGEIEARLHEQAEVREAAVAVQEGVNGKHLVGYLVP 4172
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|
gi 573989775 575 LDPDHAVKWTPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQL 624
Cdd:PRK05691 4173 HQTVLAQGALLERIKQRLRAELPDYMVPLHWLWLDRLPLNANGKLDRKAL 4222
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
230-494 |
2.05e-10 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 63.68 E-value: 2.05e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 230 MLYTSGTTNRPKGVLLPEPVITAQCRSL---LQAWE--YSPADRLLHVLPLHHI-------------------HGTINAV 285
Cdd:PLN02430 225 IMYTSGTSGDPKGVVLTHEAVATFVRGVdlfMEQFEdkMTHDDVYLSFLPLAHIldrmieeyffrkgasvgyyHGDLNAL 304
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 286 ------LTP-LFAGssVEFMFPFNADAVWKRFaapflptngtaTTAPPISFFtVVPTVYSRLLA------SHKTLSP--Q 350
Cdd:PLN02430 305 rddlmeLKPtLLAG--VPRVFERIHEGIQKAL-----------QELNPRRRL-IFNALYKYKLAwmnrgySHKKASPmaD 370
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 351 MQAATS-EATSPANLRVNISGSAALPTPVKAAWAALSGGNVLlERYGMTEvgmalSCG---LAFADRVD--ASVGWPLPS 424
Cdd:PLN02430 371 FLAFRKvKAKLGGRLRLLISGGAPLSTEIEEFLRVTSCAFVV-QGYGLTE-----TLGpttLGFPDEMCmlGTVGAPAVY 444
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 425 VEARLVDMdtgdviePGHELDDHGRERVGEIQLRGPTVFREYWRNPDATaKEFVDDGgdgrgrWFKTGDV 494
Cdd:PLN02430 445 NELRLEEV-------PEMGYDPLGEPPRGEICVRGKCLFSGYYKNPELT-EEVMKDG------WFHTGDI 500
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
219-541 |
2.39e-10 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 63.30 E-value: 2.39e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 219 LADEDFSGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFM 298
Cdd:PRK06334 177 VSDKDPEDVAVILFTSGTEKLPKGVPLTHANLLANQRACLKFFSPKEDDVMMSFLPPFHAYGFNSCTLFPLLSGVPVVFA 256
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 299 F-PFNAdavwKRFAAPFLPTNGTATTAPPIsFFTVVptvysrllashktlspqMQAATSEATSPANLRVNISGSAALPTP 377
Cdd:PRK06334 257 YnPLYP----KKIVEMIDEAKVTFLGSTPV-FFDYI-----------------LKTAKKQESCLPSLRFVVIGGDAFKDS 314
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 378 VKAAWAALSGGNVLLERYGMTEVGMALSCGLAFADRVDASVGWPLPSVEARLVDMDTGDVIEPGhelddhgreRVGEIQL 457
Cdd:PRK06334 315 LYQEALKTFPHIQLRQGYGTTECSPVITINTVNSPKHESCVGMPIRGMDVLIVSEETKVPVSSG---------ETGLVLT 385
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 458 RGPTVFREYWRNPDATAkeFVDDGGDgrgRWFKTGDvairrepVGAAGRNSHrqpwaqgpmYFIQGRkTADIIKTGGEKV 537
Cdd:PRK06334 386 RGTSLFSGYLGEDFGQG--FVELGGE---TWYVTGD-------LGYVDRHGE---------LFLKGR-LSRFVKIGAEMV 443
|
....*.
gi 573989775 538 S--ALE 541
Cdd:PRK06334 444 SleALE 449
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
61-624 |
2.10e-09 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 60.95 E-value: 2.10e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 61 LPRLpiFEAVAAHDPTSTAVVHAlsGRRFTYGELLGDVARARERLREAVDGKPLtgeRVAFLIENSydyvvPEAhsrdVV 140
Cdd:PRK05691 1133 LPEL--LNEQARQTPERIALVWD--GGSLDYAELHAQANRLAHYLRDKGVGPDV---CVAIAAERS-----PQL----LV 1196
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 141 MFLAVMAARAIAVPLSPPFPAPELQYILDQSAASVLvaspkfaakarevlatdLQQKPVYFELPKHTGSSHGAKEAVSLA 220
Cdd:PRK05691 1197 GLLAILKAGGAYVPLDPDYPAERLAYMLADSGVELL-----------------LTQSHLLERLPQAEGVSAIALDSLHLD 1259
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 221 DEDFSGAGLML---------YTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHhIHGTINAVLTPLFA 291
Cdd:PRK05691 1260 SWPSQAPGLHLhgdnlayviYTSGSTGQPKGVGNTHAALAERLQWMQATYALDDSDVLMQKAPIS-FDVSVWECFWPLIT 1338
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 292 GSSVEFMFPfnADAVWKRFAAPFLPTNGTATtappISFftvVPTVYSRLLAShktlsPQMQAATSeatspanLRVNISGS 371
Cdd:PRK05691 1339 GCRLVLAGP--GEHRDPQRIAELVQQYGVTT----LHF---VPPLLQLFIDE-----PLAAACTS-------LRRLFSGG 1397
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 372 AALPTPVKAAWAALSGGNVLLERYGMTEVGMALS---CGLAFADRVdaSVGWPLPSVEARLVDmdtgdviepgHELDDHG 448
Cdd:PRK05691 1398 EALPAELRNRVLQRLPQVQLHNRYGPTETAINVThwqCQAEDGERS--PIGRPLGNVLCRVLD----------AELNLLP 1465
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 449 RERVGEIQLRGPTVFREYWRNPDATAKEFVDDG-GDGRGRWFKTGDvairrepvgaagrnshRQPW-AQGPMYFIqGRKT 526
Cdd:PRK05691 1466 PGVAGELCIGGAGLARGYLGRPALTAERFVPDPlGEDGARLYRTGD----------------RARWnADGALEYL-GRLD 1528
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 527 ADIiKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGaviILDPDHAVKWTPLEMRRALKSRLAGYKIPQVMK 606
Cdd:PRK05691 1529 QQV-KLRGFRVEPEEIQARLLAQPGVAQAAVLVREGAAGAQLVG---YYTGEAGQEAEAERLKAALAAELPEYMVPAQLI 1604
|
570
....*....|....*...
gi 573989775 607 IVNHIPRNAMGKINKKQL 624
Cdd:PRK05691 1605 RLDQMPLGPSGKLDRRAL 1622
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
142-602 |
4.30e-09 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 59.11 E-value: 4.30e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 142 FLAVMAARAIAVPLSPPFPAPELQYILDQSAASvlvaspkfaakarevLATDLQQKPVYFELPKHTGSSHGAKEAVSLAD 221
Cdd:PRK09029 70 YLALLQCGARVLPLNPQLPQPLLEELLPSLTLD---------------FALVLEGENTFSALTSLHLQLVEGAHAVAWQP 134
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 222 EDFsgAGLMLyTSGTTNRPKGVllpepVITAQ-----CRSLLQAWEYSPADRLLHVLPLHHIHGT-InaVLTPLFAGSSV 295
Cdd:PRK09029 135 QRL--ATMTL-TSGSTGLPKAA-----VHTAQahlasAEGVLSLMPFTAQDSWLLSLPLFHVSGQgI--VWRWLYAGATL 204
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 296 EFmfpfnadavwkRFAAPFLPTNGTATTAppisffTVVPTVYSRLLASHKTlspqmqaatseatsPANLRVNISGSAALP 375
Cdd:PRK09029 205 VV-----------RDKQPLEQALAGCTHA------SLVPTQLWRLLDNRSE--------------PLSLKAVLLGGAAIP 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 376 TPVKAAWAAL-----SGgnvllerYGMTEvgMALScglAFADRVDAS--VGWPLPSVEARLVDmdtgdviepghelddhg 448
Cdd:PRK09029 254 VELTEQAEQQgircwCG-------YGLTE--MAST---VCAKRADGLagVGSPLPGREVKLVD----------------- 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 449 rervGEIQLRGPTVFREYWRnpDATAKEFVDDGGdgrgrWFKTGDvairrepvgaagrnshRQPWAQGPMYfIQGRktAD 528
Cdd:PRK09029 305 ----GEIWLRGASLALGYWR--QGQLVPLVNDEG-----WFATRD----------------RGEWQNGELT-ILGR--LD 354
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 573989775 529 ---IikTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVKWTPLEmrraLKSRLAGYKIP 602
Cdd:PRK09029 355 nlfF--SGGEGIQPEEIERVINQHPLVQQVFVVPVADAEFGQRPVAVVESDSEAAVVNLAEW----LQDKLARFQQP 425
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
147-624 |
1.47e-08 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 57.35 E-value: 1.47e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 147 AARAIAVPLSPPFPAPELQYILDQSAASVLV---ASPKFAAKarevlatdlqqkpvyfELPKHTGSS----HGAKEAVSL 219
Cdd:PRK08308 31 AGNRFAVCLKDPFDIITLVFFLKEKGASVLPihpDTPKEAAI----------------RMAKRAGCHgllyGESDFTKLE 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 220 ADEDFSGAGLML-YTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLfagssvefm 298
Cdd:PRK08308 95 AVNYLAEEPSLLqYSSGTTGEPKLIRRSWTEIDREIEAYNEALNCEQDETPIVACPVTHSYGLICGVLAAL--------- 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 299 fpfnadavwKRFAAPFLPTNGTATTAPPISFFTVVPTVYSrllashktlSPQMQAATSEATsPANLR---VNISGsaalp 375
Cdd:PRK08308 166 ---------TRGSKPVIITNKNPKFALNILRNTPQHILYA---------VPLMLHILGRLL-PGTFQfhaVMTSG----- 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 376 TPVKAAWAAL--SGGNVLLERYGMTEVG-MALSCGLAFADrvdaSVGWPLPSVearlvDMDTGDviepghelddhGRERV 452
Cdd:PRK08308 222 TPLPEAWFYKlrERTTYMMQQYGCSEAGcVSICPDMKSHL----DLGNPLPHV-----SVSAGS-----------DENAP 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 453 GEIQLrgptvfreywrnpdatakefvddggdgrgrwfKTGDVAIRREPVGAAGrnshrqpwAQGPMYFIqGRkTADIIKT 532
Cdd:PRK08308 282 EEIVV--------------------------------KMGDKEIFTKDLGYKS--------ERGTLHFM-GR-MDDVINV 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 533 GGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIIldPDHAVkwTPLEMRRALKSRLAGYKIPQVMKIVNHIP 612
Cdd:PRK08308 320 SGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVI--SHEEI--DPVQLREWCIQHLAPYQVPHEIESVTEIP 395
|
490
....*....|..
gi 573989775 613 RNAMGKINKKQL 624
Cdd:PRK08308 396 KNANGKVSRKLL 407
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
30-638 |
6.21e-08 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 55.65 E-value: 6.21e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 30 RNNIRVALPCMRPLAHHRLRMLSTTTTTASTLPRL--PIFEAVAAHDPTSTAVVhaLSGRRFTYGELLGDVAR-ARERLR 106
Cdd:PRK08279 4 LMDLAARLPRRLPDLPGILRGLKRTALITPDSKRSlgDVFEEAAARHPDRPALL--FEDQSISYAELNARANRyAHWAAA 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 107 EAVdGKpltGERVAFLIENSydyvvPEahsrdvvmFLAVMAAR----AIAVPLSPPFPAPELQYILDQSAASVLVASPKF 182
Cdd:PRK08279 82 RGV-GK---GDVVALLMENR-----PE--------YLAAWLGLaklgAVVALLNTQQRGAVLAHSLNLVDAKHLIVGEEL 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 183 AAKAREVLATDLQQKPVYFELPKHTGSSHGAKEAVSLADEDFSGA------------GLMLYTSGTTNRPKGVLLPEPVI 250
Cdd:PRK08279 145 VEAFEEARADLARPPRLWVAGGDTLDDPEGYEDLAAAAAGAPTTNpasrsgvtakdtAFYIYTSGTTGLPKAAVMSHMRW 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 251 TAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAVWKRFAApflpTNGTAttappisFf 330
Cdd:PRK08279 225 LKAMGGFGGLLRLTPDDVLYCCLPLYHNTGGTVAWSSVLAAGATLALRRKFSASRFWDDVRR----YRATA-------F- 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 331 tvvptVY----SRLLashktlspqMQAATSEATSPANLRVnISGSAALPTpVKAAWAALSGGNVLLERYGMTEvgmalsC 406
Cdd:PRK08279 293 -----QYigelCRYL---------LNQPPKPTDRDHRLRL-MIGNGLRPD-IWDEFQQRFGIPRILEFYAASE------G 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 407 GLAF--ADRVDASVGWpLPSVEA---RLV--DMDTGDVIEpghelDDHGR----------ERVGEIQLRGPtvFREYwRN 469
Cdd:PRK08279 351 NVGFinVFNFDGTVGR-VPLWLAhpyAIVkyDVDTGEPVR-----DADGRcikvkpgevgLLIGRITDRGP--FDGY-TD 421
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 470 PDATAKEFVDDG---GDgrgRWFKTGDVaIRREpvgaagrnshrqpwAQGPMYFIQgrKTADIIKTGGEKVSALEVEREL 546
Cdd:PRK08279 422 PEASEKKILRDVfkkGD---AWFNTGDL-MRDD--------------GFGHAQFVD--RLGDTFRWKGENVATTEVENAL 481
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 547 LSLPQVAEAAVVAVPSGNWGQKVG-AVIILDPDHAVKWTplEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLV 625
Cdd:PRK08279 482 SGFPGVEEAVVYGVEVPGTDGRAGmAAIVLADGAEFDLA--ALAAHLYERLPAYAVPLFVRLVPELETTGTFKYRKVDLR 559
|
650
....*....|...
gi 573989775 626 KAIFVEETSGDEL 638
Cdd:PRK08279 560 KEGFDPSKVDDPL 572
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
116-496 |
6.65e-08 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 55.52 E-value: 6.65e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 116 GERVAFLIENSYDYVVPeahsrdvvmFLAVMAARAIAVPL-SPPFP--APELQYILDQSAASVLVASPKFAAKAREVLAT 192
Cdd:PRK12476 92 GDRVAILAPQGIDYVAG---------FFAAIKAGTIAVPLfAPELPghAERLDTALRDAEPTVVLTTTAAAEAVEGFLRN 162
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 193 -DLQQKP---VYFELPKHTGSSHgakEAVSLADEDFSgagLMLYTSGTTNRPKGVllpEPVITAQCRSLLQA-WEYSPAD 267
Cdd:PRK12476 163 lPRLRRPrviAIDAIPDSAGESF---VPVELDTDDVS---HLQYTSGSTRPPVGV---EITHRAVGTNLVQMiLSIDLLD 233
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 268 RLLHV---LPLHHIHGtINAVLTPLFAGSSVEFMFP---FNADAVWKRFAAPFLPTNGTATTAPPISFftvvptvysrLL 341
Cdd:PRK12476 234 RNTHGvswLPLYHDMG-LSMIGFPAVYGGHSTLMSPtafVRRPQRWIKALSEGSRTGRVVTAAPNFAY----------EW 302
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 342 ASHKTLSPQMQAAT---------SEATSPANLRVNISGSA--ALP-TPVKAAwaalsggnvllerYGMTEVGMALSC--- 406
Cdd:PRK12476 303 AAQRGLPAEGDDIDlsnvvliigSEPVSIDAVTTFNKAFApyGLPrTAFKPS-------------YGIAEATLFVATiap 369
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 407 --------------GLAFADRVDA---------SVGWPLPSVEARLVDMDTGDVIEPGHelddhgrerVGEIQLRGPTVF 463
Cdd:PRK12476 370 daepsvvyldreqlGAGRAVRVAAdapnavahvSCGQVARSQWAVIVDPDTGAELPDGE---------VGEIWLHGDNIG 440
|
410 420 430 440
....*....|....*....|....*....|....*....|....*
gi 573989775 464 REYWRNPDATAKEFVD------------DGGDGRGRWFKTGDVAI 496
Cdd:PRK12476 441 RGYWGRPEETERTFGAklqsrlaegshaDGAADDGTWLRTGDLGV 485
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
142-626 |
7.97e-08 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 55.13 E-value: 7.97e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 142 FLAVMAARAIAVpLSPPFPAPELQYILDQSAASVLVASPKFAAKAREVLA--TDLQQKPV----YFELPKHTGSSHGAKE 215
Cdd:cd05915 67 FAVPGMGAVLHT-ANPRLSPKEIAYILNHAEDKVLLFDPNLLPLVEAIRGelKTVQHFVVmdekAPEGYLAYEEALGEEA 145
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 216 AVSLADEDFSGAglMLYTSGTTNRPKGVLLPE--PVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGS 293
Cdd:cd05915 146 DPVRVPERAACG--MAYTTGTTGLPKGVVYSHraLVLHSLAASLVDGTALSEKDVVLPVVPMFHVNAWCLPYAATLVGAK 223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 294 SVEFMFPFNADAVWKrfaapflptngtATTAPPISFFTVVPTVYSRLLASHKTLspqmqaatsEATSPANLRVNISGSAa 373
Cdd:cd05915 224 QVLPGPRLDPASLVE------------LFDGEGVTFTAGVPTVWLALADYLEST---------GHRLKTLRRLVVGGSA- 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 374 lPTPVKAAWAALSGGNVLLErYGMTEV-GMALSCgLAFADrvdasvgW-PLPSVEARLVDMDTG--------DVIEPGHE 443
Cdd:cd05915 282 -APRSLIARFERMGVEVRQG-YGLTETsPVVVQN-FVKSH-------LeSLSEEEKLTLKAKTGlpiplvrlRVADEEGR 351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 444 LDDHGRERVGEIQLRGPTVFREYWRNPDAT-AKEFvddggdgRGRWFKTGDVAIRrEPVGaagrnshrqpwaqgpmyFIQ 522
Cdd:cd05915 352 PVPKDGKALGEVQLKGPWITGGYYGNEEATrSALT-------PDGFFRTGDIAVW-DEEG-----------------YVE 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 523 GR-KTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVPSGNWGQKVGAVIILDPDHAVKWTPLEMrraLKSRLAGYK- 600
Cdd:cd05915 407 IKdRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVVVPRGEKPTPEELNEH---LLKAGFAKWq 483
|
490 500
....*....|....*....|....*.
gi 573989775 601 IPQVMKIVNHIPRNAMGKINKKQLVK 626
Cdd:cd05915 484 LPDAYVFAEEIPRTSAGKFLKRALRE 509
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
225-550 |
1.82e-07 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 54.35 E-value: 1.82e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 225 SGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAW-EYSPADRLLHVLPLHHIHgTINAVLTPLFAGSSVEFMFPFN- 302
Cdd:PLN02387 250 NDIAVIMYTSGSTGLPKGVMMTHGNIVATVAGVMTVVpKLGKNDVYLAYLPLAHIL-ELAAESVMAAVGAAIGYGSPLTl 328
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 303 ---ADAVWKrfaapflPTNGTATTAPPiSFFTVVPTVYSRLL-ASHKTLSPQMQAATSEATSPANLRVN-ISGS------ 371
Cdd:PLN02387 329 tdtSNKIKK-------GTKGDASALKP-TLMTAVPAILDRVRdGVRKKVDAKGGLAKKLFDIAYKRRLAaIEGSwfgawg 400
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 372 -------AALPTPVKAAWAA-----LSGG-----------NVLL-----ERYGMTEvgmalSC-GLAFADRVDASVGW-- 420
Cdd:PLN02387 401 lekllwdALVFKKIRAVLGGrirfmLSGGaplsgdtqrfiNICLgapigQGYGLTE-----TCaGATFSEWDDTSVGRvg 475
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 421 -PLPSVEARLVDMDTGdviepGHELDDHGRERvGEIQLRGPTVFREYWRNPDATAKEF-VDDggdgRG-RWFKTGDVAir 497
Cdd:PLN02387 476 pPLPCCYVKLVSWEEG-----GYLISDKPMPR-GEIVIGGPSVTLGYFKNQEKTDEVYkVDE----RGmRWFYTGDIG-- 543
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....
gi 573989775 498 repvgaagrnshrQPWAQGPMYFIQGRKtaDIIK-TGGEKVSALEVERELLSLP 550
Cdd:PLN02387 544 -------------QFHPDGCLEIIDRKK--DIVKlQHGEYVSLGKVEAALSVSP 582
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
197-626 |
3.91e-07 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 52.81 E-value: 3.91e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 197 KPVYFEL--PKHTGSShgaKEAVSLADEDFSGAGLMLYTSGTTNRPKgvllpePVITAQCRSLLQA------WEYSPADR 268
Cdd:cd05939 77 KALIFNLldPLLTQSS---TEPPSQDDVNFRDKLFYIYTSGTTGLPK------AAVIVHSRYYRIAagayyaFGMRPEDV 147
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 269 LLHVLPLHHIHGTINAVLTPLFAGSSVEFMFPFNADAVWKRFAApflpTNGTATtappisffTVVPTVYSRLLAshktls 348
Cdd:cd05939 148 VYDCLPLYHSAGGIMGVGQALLHGSTVVIRKKFSASNFWDDCVK----YNCTIV--------QYIGEICRYLLA------ 209
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 349 pqmqAATSEATSPANLRVNIsGSAALPTpvkaAWAALSGG-NV--LLERYGMTEVGMAL--------SCGlaFADRVDAS 417
Cdd:cd05939 210 ----QPPSEEEQKHNVRLAV-GNGLRPQ----IWEQFVRRfGIpqIGEFYGATEGNSSLvnidnhvgACG--FNSRILPS 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 418 VgWPLPSVEarlVDMDTGDVI-------------EPGhelddhgrERVGEIQLRGPTVFREYWRNPDATAKEFVDD---G 481
Cdd:cd05939 279 V-YPIRLIK---VDEDTGELIrdsdglcipcqpgEPG--------LLVGKIIQNDPLRRFDGYVNEGATNKKIARDvfkK 346
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 482 GDgrgRWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIQgrKTADIIKTGGEKVSALEVERELLSLPQVAEAAVVAVP 561
Cdd:cd05939 347 GD---SAFLSGDVLVMDE---------------LGYLYFKD--RTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVYGVE 406
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 573989775 562 SGNWGQKVGAVIILDPDHAVKwtPLEMRRALKSRLAGYKIPQVMKIVNHIPRNAMGKINKKQLVK 626
Cdd:cd05939 407 VPGVEGRAGMAAIVDPERKVD--LDRFSAVLAKSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQK 469
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
139-624 |
1.19e-06 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 51.32 E-value: 1.19e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 139 VVMFLAVMAARAIAVPLSPPFPAPELQYILDQSAASVLVASPKfaakarevlatdLQQKPVYFELPK-HTGSSHGAKEAV 217
Cdd:cd17654 55 PVAILAILFLGAAYAPIDPASPEQRSLTVMKKCHVSYLLQNKE------------LDNAPLSFTPEHrHFNIRTDECLAY 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 218 SLadedfsgaglmlYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLH------HIHGTINAVLTPLFA 291
Cdd:cd17654 123 VI------------HTSGTTGTPKIVAVPHKCILPNIQHFRSLFNITSEDILFLTSPLTfdpsvvEIFLSLSSGATLLIV 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 292 GSSVEFMFPFNADAVWKRFAapflPTNGTATtappisfftvvPTVYSRLLAShktlspQMQAATSEATSpaNLRVNISGS 371
Cdd:cd17654 191 PTSVKVLPSKLADILFKRHR----ITVLQAT-----------PTLFRRFGSQ------SIKSTVLSATS--SLRVLALGG 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 372 AALPTPV-KAAWAALSGGNVLLERYGMTEVgmalsCGLAFADRV---DASVGWPLPSVearlvdmdtGDVIEpghELDDH 447
Cdd:cd17654 248 EPFPSLViLSSWRGKGNRTRIFNIYGITEV-----SCWALAYKVpeeDSPVQLGSPLL---------GTVIE---VRDQN 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 448 GRERVGEIQLRGptVFREYWRNPDATAKEfvddggdgrGRWFKTGDVaIRREpvgaagrnshrqpwaQGPMYFIqGRKTa 527
Cdd:cd17654 311 GSEGTGQVFLGG--LNRVCILDDEVTVPK---------GTMRATGDF-VTVK---------------DGELFFL-GRKD- 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 528 DIIKTGGEKVSALEVERELLSLPQVAEAAVvavpsgNW--GQKVGAVIILDPDHAvkwtpLEMRRALKSRLAGYKIPQVM 605
Cdd:cd17654 362 SQIKRRGKRINLDLIQQVIESCLGVESCAV------TLsdQQRLIAFIVGESSSS-----RIHKELQLTLLSSHAIPDTF 430
|
490
....*....|....*....
gi 573989775 606 KIVNHIPRNAMGKINKKQL 624
Cdd:cd17654 431 VQIDKLPLTSHGKVDKSEL 449
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
391-626 |
1.22e-06 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 51.43 E-value: 1.22e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 391 LLER---------YGMTEVGMALScGLAFADRVDAS-----VGWPLPSVEArLVDMDTGDVIEPGHElddhgrervGEIQ 456
Cdd:PRK04813 280 LLERfpsatiyntYGPTEATVAVT-SIEITDEMLDQykrlpIGYAKPDSPL-LIIDEEGTKLPDGEQ---------GEIV 348
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 457 LRGPTVFREYWRNPDATAKEFVDDggDGRgRWFKTGDVAIrrepvgaagrnshrqpwAQGPMYFIQGRktADI-IKTGGE 535
Cdd:PRK04813 349 ISGPSVSKGYLNNPEKTAEAFFTF--DGQ-PAYHTGDAGY-----------------LEDGLLFYQGR--IDFqIKLNGY 406
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 536 KVSALEVERELLSLPQVaeaavvavpsgnwgqKVGAVIILDPDHAVKW-------------TPLEMRRALKSRLAG---- 598
Cdd:PRK04813 407 RIELEEIEQNLRQSSYV---------------ESAVVVPYNKDHKVQYliayvvpkeedfeREFELTKAIKKELKErlme 471
|
250 260 270
....*....|....*....|....*....|
gi 573989775 599 YKIPQvmKIV--NHIPRNAMGKINKKQLVK 626
Cdd:PRK04813 472 YMIPR--KFIyrDSLPLTPNGKIDRKALIE 499
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
73-245 |
7.25e-06 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 49.19 E-value: 7.25e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 73 HDPTSTAVVHALSG---RRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENsydyvVPEAhsrdVVMFLAVMAAR 149
Cdd:cd05943 80 ADADDPAAIYAAEDgerTEVTWAELRRRVARLAAALRALGVKP---GDRVAGYLPN-----IPEA----VVAMLATASIG 147
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 150 AIAVPLSPPFPAPElqyILD---QSAASVLVASP--KFAAK---AREVLATDLQQKP-------VYFELPKHTGSSHGAK 214
Cdd:cd05943 148 AIWSSCSPDFGVPG---VLDrfgQIEPKVLFAVDayTYNGKrhdVREKVAELVKGLPsllavvvVPYTVAAGQPDLSKIA 224
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|..
gi 573989775 215 EAVSLADE--DFSGAGL------------MLYTSGTTNRPK-------GVLL 245
Cdd:cd05943 225 KALTLEDFlaTGAAGELefeplpfdhplyILYSSGTTGLPKcivhgagGTLL 276
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
229-624 |
1.39e-05 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 47.95 E-value: 1.39e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 229 LMLY-TSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPADRLLHVLPLHHIHGTINAVLTPLFAGSSVefmFPFNA---D 304
Cdd:cd05974 88 MLLYfTSGTTSKPKLVEHTHRSYPVGHLSTMYWIGLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATV---FLFNYarfD 164
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 305 AvwKRFAAPFLPTNGTATTAPPisffTVVPTVYSRLLASHKTlSPQMQAATSEATSPANLRVnisgsaalptpVKAAWaa 384
Cdd:cd05974 165 A--KRVLAALVRYGVTTLCAPP----TVWRMLIQQDLASFDV-KLREVVGAGEPLNPEVIEQ-----------VRRAW-- 224
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 385 lsgGNVLLERYGMTEVgMALSCGLAFADRVDASVGWPLPSVEARLVDMDTGDVIEPGHELDDHGRERVGEIQlrgptvfr 464
Cdd:cd05974 225 ---GLTIRDGYGQTET-TALVGNSPGQPVKAGSMGRPLPGYRVALLDPDGAPATEGEVALDLGDTRPVGLMK-------- 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 465 EYWRNPDATAkefvddgGDGRGRWFKTGDVAIRREpvgaagrnshrqpwaQGPMYFIqGRkTADIIKTGGEKVSALEVER 544
Cdd:cd05974 293 GYAGDPDKTA-------HAMRGGYYRTGDIAMRDE---------------DGYLTYV-GR-ADDVFKSSDYRISPFELES 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 545 ELLSLPQVAEAAVVAVPSGNWGQKVGAVIIL------DPDhavkwTPLEMRRALKSRLAGYKIPQVMKIVnHIPRNAMGK 618
Cdd:cd05974 349 VLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLragyepSPE-----TALEIFRFSRERLAPYKRIRRLEFA-ELPKTISGK 422
|
....*.
gi 573989775 619 INKKQL 624
Cdd:cd05974 423 IRRVEL 428
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
86-293 |
5.69e-05 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 46.13 E-value: 5.69e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 86 GRRFTYGELLGDVARARERLREAVDGKPltGERVAFLIENSYDYVVpeahsrdvvMFLAVMAARAIAVPLSPPFPAPELQ 165
Cdd:cd05938 3 GETYTYRDVDRRSNQAARALLAHAGLRP--GDTVALLLGNEPAFLW---------IWLGLAKLGCPVAFLNTNIRSKSLL 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 166 YILDQSAASVLVASPKFAAKAREVLATdLQQKPVYFELPKHTGSSHGAK---EAVSLADED-----------FSGAGLML 231
Cdd:cd05938 72 HCFRCCGAKVLVVAPELQEAVEEVLPA-LRADGVSVWYLSHTSNTEGVIsllDKVDAASDEpvpaslrahvtIKSPALYI 150
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 573989775 232 YTSGTTNRPK-GVLLPEPVItaQCRSLLQAWEYSPADRLLHVLPLHH-------IHGTINA----VLTPLFAGS 293
Cdd:cd05938 151 YTSGTTGLPKaARISHLRVL--QCSGFLSLCGVTADDVIYITLPLYHssgfllgIGGCIELgatcVLKPKFSAS 222
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
73-245 |
4.24e-04 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 43.25 E-value: 4.24e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 73 HDPTSTAVVHALSG---RRFTYGELLGDVARARERLREAVDGKpltGERVAFLIENsydyvVPEAhsrdVVMFLAVMAAR 149
Cdd:PRK03584 96 RRDDRPAIIFRGEDgprRELSWAELRRQVAALAAALRALGVGP---GDRVAAYLPN-----IPET----VVAMLATASLG 163
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 150 AIAVPLSPPFPApelQYILD---QSAASVLVASP--KFAAKA---REVLATDLQQKP-----VYFELPKHTGSSHGAKEA 216
Cdd:PRK03584 164 AIWSSCSPDFGV---QGVLDrfgQIEPKVLIAVDgyRYGGKAfdrRAKVAELRAALPslehvVVVPYLGPAAAAAALPGA 240
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|
gi 573989775 217 VSLAD--EDFSGAGL------------MLYTSGTTNRPK-------GVLL 245
Cdd:PRK03584 241 LLWEDflAPAEAAELefepvpfdhplwILYSSGTTGLPKcivhghgGILL 290
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
218-507 |
7.26e-04 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 42.45 E-value: 7.26e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 218 SLADEDFSGAGLMLYTSGTTNRPKGVLLPEPVITAQCRSLLQAWEYSPA-DRLLHVLPLHHIHGTInAVLTPLFAGSSVe 296
Cdd:PRK05851 145 SLTPPDSGGPAVLQGTAGSTGTPRTAILSPGAVLSNLRGLNARVGLDAAtDVGCSWLPLYHDMGLA-FLLTAALAGAPL- 222
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 297 FMFPFNADAvwkrfAAPF-----LPTNGTATTAPPISFFTVVPTVYSRLlaSHKTLSpqmqaatseatspaNLRVNISGS 371
Cdd:PRK05851 223 WLAPTTAFS-----ASPFrwlswLSDSRATLTAAPNFAYNLIGKYARRV--SDVDLG--------------ALRVALNGG 281
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 372 AalptPVK-------AAWAALSGGN--VLLERYGMTEVGMALSC-----GLAFADRVD---------ASVGWPLPSVEAR 428
Cdd:PRK05851 282 E----PVDcdgferfATAMAPFGFDagAAAPSYGLAESTCAVTVpvpgiGLRVDEVTTddgsgarrhAVLGNPIPGMEVR 357
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 429 lvdmdtgdvIEPGHELDDHGRERVGEIQLRGPTVFREYwrnpdatakeFVDDGGDgRGRWFKTGDVAI-----------R 497
Cdd:PRK05851 358 ---------ISPGDGAAGVAGREIGEIEIRGASMMSGY----------LGQAPID-PDDWFPTGDLGYlvdgglvvcgrA 417
|
330
....*....|
gi 573989775 498 REPVGAAGRN 507
Cdd:PRK05851 418 KELITVAGRN 427
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
227-308 |
5.08e-03 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 39.64 E-value: 5.08e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573989775 227 AGLMLYTSGTTNrpkgvlLPEPVITAQCRSLLQ-AWEYS-----PADRLLHVLPLHHIHGTINAVLTPLFAGSSVEFMFP 300
Cdd:cd05940 83 AALYIYTSGTTG------LPKAAIISHRRAWRGgAFFAGsggalPSDVLYTCLPLYHSTALIVGWSACLASGATLVIRKK 156
|
....*...
gi 573989775 301 FNADAVWK 308
Cdd:cd05940 157 FSASNFWD 164
|
|
| |
