|
Name |
Accession |
Description |
Interval |
E-value |
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
27-666 |
0e+00 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 1165.79 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 27 QYEKLYRRSVEEPHEFWGDIAKEFYWKShhsgPFLQYnFDVTKGKIFVEWMKGSTTNICYNLLDRNVneKKLGDKIAFYW 106
Cdd:cd05966 2 QYKELYKQSIEDPEEFWGEIAKELDWFK----PWDKV-LDWSKGPPFIKWFEGGKLNISYNCLDRHL--KERGDKVAIIW 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 107 EGNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILD 186
Cdd:cd05966 75 EGDEPDQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGFSAESLADRIND 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 187 SNCSLLVTADAFYRGDKLISLKQIADDALQKCkekgFLVRNCIVVNHLGREelvsdgttsqsppvkrlcqdvqMPWNSDV 266
Cdd:cd05966 155 AQCKLVITADGGYRGGKVIPLKEIVDEALEKC----PSVEKVLVVKRTGGE----------------------VPMTEGR 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 267 DLWWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPEDIYWCTGDIGWITGH 346
Cdd:cd05966 209 DLWWHDLMAKQSPECEPEWMDSEDPLFILYTSGSTGKPKGVVHTTGGYLLYAATTFKYVFDYHPDDIYWCTADIGWITGH 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 347 SYLTYGPLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEPINPE 426
Cdd:cd05966 289 SYIVYGPLANGATTVMFEGTPTYPDPGRYWDIVEKHKVTIFYTAPTAIRALMKFGDEWVKKHDLSSLRVLGSVGEPINPE 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 427 AWLWYYHVIGDERCPIVDTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPWPGI 506
Cdd:cd05966 369 AWMWYYEVIGKERCPIVDTWWQTETGGIMITPLPGATPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPWPGM 448
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 507 MRTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHP 586
Cdd:cd05966 449 ARTIYGDHERYEDTYFSKFPGYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHD 528
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 587 VKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAKNDRDLGDTSTVAD 666
Cdd:cd05966 529 IKGEAIYAFVTLKDGEEPSDELRKELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKIMRRILRKIAAGEEELGDTSTLAD 608
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
9-677 |
0e+00 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 1118.30 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 9 LYRAPAALAREAHVSsLAQYEKLYRRSVEEPHEFWGDIAKEFYWkshhsgpFLQYNFDVTKGKIFVEWMKGSTTNICYNL 88
Cdd:PRK00174 1 VFPPPAEFAANALID-MEQYKALYQESVEDPEGFWAEQAKRLDW-------FKPFDTVLDWNAPFIKWFEDGELNVSYNC 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 89 LDRNVNEKklGDKIAFYWEGNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALH 168
Cdd:PRK00174 73 LDRHLKTR--GDKVAIIWEGDDPGDSRKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVH 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 169 SIVFAGFSAESLCERILDSNCSLLVTADAFYRGDKLISLKQIADDALQKCKEkgflVRNCIVVnhlgreelvsdgttsqs 248
Cdd:PRK00174 151 SVVFGGFSAEALADRIIDAGAKLVITADEGVRGGKPIPLKANVDEALANCPS----VEKVIVV----------------- 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 249 ppvKRLCQDVqmPWNSDVDLWWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDY 328
Cdd:PRK00174 210 ---RRTGGDV--DWVEGRDLWWHELVAGASDECEPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAAMTMKYVFDY 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 329 HPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKC 408
Cdd:PRK00174 285 KDGDVYWCTADVGWVTGHSYIVYGPLANGATTLMFEGVPNYPDPGRFWEVIDKHKVTIFYTAPTAIRALMKEGDEHPKKY 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 409 SRESLKILGTVGEPINPEAWLWYYHVIGDERCPIVDTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQE 488
Cdd:PRK00174 365 DLSSLRLLGSVGEPINPEAWEWYYKVVGGERCPIVDTWWQTETGGIMITPLPGATPLKPGSATRPLPGIQPAVVDEEGNP 444
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 489 LEGEAEGFLVFKQPWPGIMRTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESA 568
Cdd:PRK00174 445 LEGGEGGNLVIKDPWPGMMRTIYGDHERFVKTYFSTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESA 524
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 569 LAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:PRK00174 525 LVAHPKVAEAAVVGRPDDIKGQGIYAFVTLKGGEEPSDELRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGKIMRRIL 604
|
650 660
....*....|....*....|....*....
gi 1394780554 649 RQIAKNDRDLGDTSTVADQSVISLLFNNR 677
Cdd:PRK00174 605 RKIAEGEEILGDTSTLADPSVVEKLIEAR 633
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
22-673 |
0e+00 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 1028.35 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 22 VSSLAQYEKLYRRSVEEPHEFWGDIAKE-FYWKShhsgPF---LQYNFDVtkgkiFVEWMKGSTTNICYNLLDRNVneKK 97
Cdd:TIGR02188 1 IANLEQYKELYEESIEDPDKFWAKLARElLDWFK----PFtkvLDWSFPP-----FYKWFVGGELNVSYNCVDRHL--EA 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 98 LGDKIAFYWEGNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSA 177
Cdd:TIGR02188 70 RPDKVAIIWEGDEPGEVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVFGGFSA 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 178 ESLCERILDSNCSLLVTADAFYRGDKLISLKQIADDALQKCKEKgflVRNCIVVNHLGREElvsdgttsqsppvkrlcqd 257
Cdd:TIGR02188 150 EALADRINDAGAKLVITADEGLRGGKVIPLKAIVDEALEKCPVS---VEHVLVVRRTGNPV------------------- 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 258 vqMPWNSDVDLWWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPEDIYWCT 337
Cdd:TIGR02188 208 --VPWVEGRDVWWHDLMAKASAYCEPEPMDSEDPLFILYTSGSTGKPKGVLHTTGGYLLYAAMTMKYVFDIKDGDIFWCT 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 338 GDIGWITGHSYLTYGPLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILG 417
Cdd:TIGR02188 286 ADVGWITGHSYIVYGPLANGATTVMFEGVPTYPDPGRFWEIIEKHKVTIFYTAPTAIRALMRLGDEWVKKHDLSSLRLLG 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 418 TVGEPINPEAWLWYYHVIGDERCPIVDTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAE-GF 496
Cdd:TIGR02188 366 SVGEPINPEAWMWYYKVVGKERCPIVDTWWQTETGGIMITPLPGATPTKPGSATLPFFGIEPAVVDEEGNPVEGPGEgGY 445
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 497 LVFKQPWPGIMRTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAIS 576
Cdd:TIGR02188 446 LVIKQPWPGMLRTIYGDHERFVDTYFSPFPGYYFTGDGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVA 525
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 577 EAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAKNDR 656
Cdd:TIGR02188 526 EAAVVGIPDDIKGQAIYAFVTLKDGYEPDDELRKELRKHVRKEIGPIAKPDKIRFVPGLPKTRSGKIMRRLLRKIAAGEA 605
|
650
....*....|....*...
gi 1394780554 657 D-LGDTSTVADQSVISLL 673
Cdd:TIGR02188 606 EiLGDTSTLEDPSVVEEL 623
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
1-673 |
0e+00 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 907.35 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 1 MAPEEAGrLYRAPAALAREAHVSSLAQYEKLYRRSVEEPHEFWGDIAKEFYWKSHHSG-PFLQYNFDVTKGKIFVEWMKG 79
Cdd:PLN02654 6 LASEEND-LVFPSKDFSAQALVSSPQQYMEMYKRSVDDPAGFWSDIASQFYWKQKWEGdEVCSENLDVRKGPISIEWFKG 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 80 STTNICYNLLDRNVnEKKLGDKIAFYWEGNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAML 159
Cdd:PLN02654 85 GKTNICYNCLDRNV-EAGNGDKIAIYWEGNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAML 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 160 ACARIGALHSIVFAGFSAESLCERILDSNCSLLVTADAFYRGDKLISLKQIADDALQKCKEKGFLVRNCIvvnhlgreel 239
Cdd:PLN02654 164 ACARIGAVHSVVFAGFSAESLAQRIVDCKPKVVITCNAVKRGPKTINLKDIVDAALDESAKNGVSVGICL---------- 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 240 vsdgTTSQSPPVKRlcqdVQMPWNSDVDLWWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTA 319
Cdd:PLN02654 234 ----TYENQLAMKR----EDTKWQEGRDVWWQDVVPNYPTKCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTA 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 320 ATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMK 399
Cdd:PLN02654 306 TTFKYAFDYKPTDVYWCTADCGWITGHSYVTYGPMLNGATVLVFEGAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMR 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 400 SGDEPVKKCSRESLKILGTVGEPINPEAWLWYYHVIGDERCPIVDTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVP 479
Cdd:PLN02654 386 DGDEYVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQP 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 480 AILDESGQELEGEAEGFLVFKQPWPGIMRTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHL 559
Cdd:PLN02654 466 VIVDEKGKEIEGECSGYLCVKKSWPGAFRTLYGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHR 545
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 560 LSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTR 639
Cdd:PLN02654 546 IGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTR 625
|
650 660 670
....*....|....*....|....*....|....*
gi 1394780554 640 SGKILRRVLRQIAKNDRD-LGDTSTVADQSVISLL 673
Cdd:PLN02654 626 SGKIMRRILRKIASRQLDeLGDTSTLADPGVVDQL 660
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
75-670 |
0e+00 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 865.58 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 75 EWMKGSTTNICYNLLDRNVNEKklGDKIAFYWEGnDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIEL 154
Cdd:COG0365 1 RWFVGGRLNIAYNCLDRHAEGR--GDKVALIWEG-EDGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEA 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 155 VVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTADAFYRGDKLISLKQIADDALQKCKEkgflVRNCIVVNHL 234
Cdd:COG0365 78 VIAMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLRGGKVIDLKEKVDEALEELPS----LEHVIVVGRT 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 235 GreelvsdgttsqsppvkrlcQDVQMPWnsdvDLWWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGY 314
Cdd:COG0365 154 G--------------------ADVPMEG----DLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGY 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 315 MIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAI 394
Cdd:COG0365 210 LVHAATTAKYVLDLKPGDVFWCTADIGWATGHSYIVYGPLLNGATVVLYEGRPDFPDPGRLWELIEKYGVTVFFTAPTAI 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 395 RALMKSGDEPVKKCSRESLKILGTVGEPINPEAWLWYYHVIGderCPIVDTFWQTETGGHMLTPLPAvTAMKPGSATFPF 474
Cdd:COG0365 290 RALMKAGDEPLKKYDLSSLRLLGSAGEPLNPEVWEWWYEAVG---VPIVDGWGQTETGGIFISNLPG-LPVKPGSMGKPV 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 475 FGVVPAILDESGQELEGEAEGFLVFKQPWPGIMRTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLN 554
Cdd:COG0365 366 PGYDVAVVDEDGNPVPPGEEGELVIKGPWPGMFRGYWNDPERYRETYFGRFPGWYRTGDGARRDEDGYFWILGRSDDVIN 445
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 555 VSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPG 634
Cdd:COG0365 446 VSGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQVVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDE 525
|
570 580 590
....*....|....*....|....*....|....*.
gi 1394780554 635 LPKTRSGKILRRVLRQIAKNdRDLGDTSTVADQSVI 670
Cdd:COG0365 526 LPKTRSGKIMRRLLRKIAEG-RPLGDTSTLEDPEAL 560
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
28-644 |
0e+00 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 719.36 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 28 YEKLYRRSVEEPHEFWGDIAKEFYWKSHhsgPFLQYNFDVTKGKIFVEWMKGSTTNICYNLLDRNVNEKklGDKIAFYWE 107
Cdd:cd17634 1 YETKYRQSINDPDTFWGEAGKILDWITP---YQKVKNTSFAPGAPSIKWFEDATLNLAANALDRHLREN--GDRTAIIYE 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 108 GNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDS 187
Cdd:cd17634 76 GDDTSQSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDS 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 188 NCSLLVTADAFYRGDKLISLKQIADDALQKckeKGFLVRNCIVVnhlgreelvsdgttsqsppvKRLCQDVQmpWNSDVD 267
Cdd:cd17634 156 SSRLLITADGGVRAGRSVPLKKNVDDALNP---NVTSVEHVIVL--------------------KRTGSDID--WQEGRD 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 268 LWWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHS 347
Cdd:cd17634 211 LWWRDLIAKASPEHQPEAMNAEDPLFILYTSGTTGKPKGVLHTTGGYLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHS 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 348 YLTYGPLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEPINPEA 427
Cdd:cd17634 291 YLLYGPLACGATTLLYEGVPNWPTPARMWQVVDKHGVNILYTAPTAIRALMAAGDDAIEGTDRSSLRILGSVGEPINPEA 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 428 WLWYYHVIGDERCPIVDTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPWPGIM 507
Cdd:cd17634 371 YEWYWKKIGKEKCPVVDTWWQTETGGFMITPLPGAIELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDPWPGQT 450
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 508 RTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPV 587
Cdd:cd17634 451 RTLFGDHERFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAI 530
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*..
gi 1394780554 588 KGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKIL 644
Cdd:cd17634 531 KGQAPYAYVVLNHGVEPSPELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKIM 587
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
28-670 |
0e+00 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 590.83 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 28 YEKLYRRSVEEPHEFWGDIAKEFYWKShhsgPFLQYnFDVTKGKiFVEWMKGSTTNICYNLLDRNVnEKKLGDKIAFYWE 107
Cdd:cd05967 1 YEEVYARSIAEPEAFWAEQARLIDWFK----PPEKI-LDNSNPP-FTRWFVGGRLNTCYNALDRHV-EAGRGDQIALIYD 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 108 GNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDS 187
Cdd:cd05967 74 SPVTGTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAKELASRIDDA 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 188 NCSLLVTADAFYRGDKLISLKQIADDALqkcKEKGFLVRNCIVVNhlgREELVSDgttsqsppvkrlcqdvqmPWNSDVD 267
Cdd:cd05967 154 KPKLIVTASCGIEPGKVVPYKPLLDKAL---ELSGHKPHHVLVLN---RPQVPAD------------------LTKPGRD 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 268 LWWHELMRNASKEcEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHS 347
Cdd:cd05967 210 LDWSELLAKAEPV-DCVPVAATDPLYILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIYGIKPGDVWWAASDVGWVVGHS 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 348 YLTYGPLANGATSVLFEGVPT-YPDVSRMWNIVDKYKVTKLYTSPTAIRALMKS--GDEPVKKCSRESLKILGTVGEPIN 424
Cdd:cd05967 289 YIVYGPLLHGATTVLYEGKPVgTPDPGAFWRVIEKYQVNALFTAPTAIRAIRKEdpDGKYIKKYDLSSLRTLFLAGERLD 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 425 PEAWLWYYHVIGderCPIVDTFWQTETGGHMLTPLPAVTAM--KPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQP 502
Cdd:cd05967 369 PPTLEWAENTLG---VPVIDHWWQTETGWPITANPVGLEPLpiKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIKLP 445
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 503 W-PGIMRTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVV 581
Cdd:cd05967 446 LpPGCLLTLWKNDERFKKLYLSKFPGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVV 525
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 582 SHPHPVKGECLYCFVTLRDGHEFS-KTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAkNDRDLGD 660
Cdd:cd05967 526 GVRDELKGQVPLGLVVLKEGVKITaEELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRKIA-DGEDYTI 604
|
650
....*....|
gi 1394780554 661 TSTVADQSVI 670
Cdd:cd05967 605 PSTIEDPSVL 614
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
26-670 |
0e+00 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 561.11 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 26 AQYEKLYRRSVEEPHEFWGDIAKEFYWKShhsgPFLQYnFDVTKGKiFVEWMKGSTTNICYNLLDRNVNEKklGDKIAFY 105
Cdd:PRK10524 2 MSYSEFYQRSIDDPEAFWAEQARRIDWQT----PFTQV-LDYSNPP-FARWFVGGRTNLCHNAVDRHLAKR--PEQLALI 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 106 WEGNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERIL 185
Cdd:PRK10524 74 AVSTETDEERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASHSLAARID 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 186 DSNCSLLVTADAFYRGDKLISLKQIADDALQKckekgflvrncivvnhlgreelvsdgttSQSPPVKRLCQDVQ---MPW 262
Cdd:PRK10524 154 DAKPVLIVSADAGSRGGKVVPYKPLLDEAIAL----------------------------AQHKPRHVLLVDRGlapMAR 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 263 NSDVDLWWHEL-MRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPEDIYWCTGDIG 341
Cdd:PRK10524 206 VAGRDVDYATLrAQHLGARVPVEWLESNEPSYILYTSGTTGKPKGVQRDTGGYAVALATSMDTIFGGKAGETFFCASDIG 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 342 WITGHSYLTYGPLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGE 421
Cdd:PRK10524 286 WVVGHSYIVYAPLLAGMATIMYEGLPTRPDAGIWWRIVEKYKVNRMFSAPTAIRVLKKQDPALLRKHDLSSLRALFLAGE 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 422 PINPEAWLWYYHVIGderCPIVDTFWQTETGGHMLTPLPAVTAM--KPGSATFPFFGVVPAILDE-SGQELEGEAEGFLV 498
Cdd:PRK10524 366 PLDEPTASWISEALG---VPVIDNYWQTETGWPILAIARGVEDRptRLGSPGVPMYGYNVKLLNEvTGEPCGPNEKGVLV 442
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 499 FKQPW-PGIMRTVYGNYKRFETTYFKKF-PGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAIS 576
Cdd:PRK10524 443 IEGPLpPGCMQTVWGDDDRFVKTYWSLFgRQVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVA 522
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 577 EAAVVSHPHPVKGECLYCFVTLRDGH-----EFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQI 651
Cdd:PRK10524 523 EVAVVGVKDALKGQVAVAFVVPKDSDsladrEARLALEKEIMALVDSQLGAVARPARVWFVSALPKTRSGKLLRRAIQAI 602
|
650
....*....|....*....
gi 1394780554 652 AKnDRDLGDTSTVADQSVI 670
Cdd:PRK10524 603 AE-GRDPGDLTTIEDPAAL 620
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
25-666 |
0e+00 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 528.21 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 25 LAQYEKLYRRSVEEPHEFWGDIAKEF-YWKSHHSGPFLqynfDVTKGKIFVEWMKGSTTNICYNLLDRNVNEKKlgDKIA 103
Cdd:cd05968 6 IPDLEAFLERSAEDNAWFWGEFVKDVgIEWYEPPYQTL----DLSGGKPWAAWFVGGRMNIVEQLLDKWLADTR--TRPA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 104 FYWEGNDpGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCER 183
Cdd:cd05968 80 LRWEGED-GTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATR 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 184 ILDSNCSLLVTADAFYRGDKLISLKQIADDALQKCkekgFLVRNCIVVNHLGREelvsdgttsqsppvkrlcqdvqMPWN 263
Cdd:cd05968 159 LQDAEAKALITADGFTRRGREVNLKEEADKACAQC----PTVEKVVVVRHLGND----------------------FTPA 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 264 SDVDLWWHELMrnASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPED-IYWCTgDIGW 342
Cdd:cd05968 213 KGRDLSYDEEK--ETAGDGAERTESEDPLMIIYTSGTTGKPKGTVHVHAGFPLKAAQDMYFQFDLKPGDlLTWFT-DLGW 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 343 ITGhSYLTYGPLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEP 422
Cdd:cd05968 290 MMG-PWLIFGGLILGATMVLYDGAPDHPKADRLWRMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSSLRVLGSTGEP 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 423 INPEAWLWYYHVIGDERCPIVDTFWQTETGGHMLTPLPaVTAMKPGSATFPFFGVVPAILDESGQELEGEaEGFLVFKQP 502
Cdd:cd05968 369 WNPEPWNWLFETVGKGRNPIINYSGGTEISGGILGNVL-IKPIKPSSFNGPVPGMKADVLDESGKPARPE-VGELVLLAP 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 503 WPGIMRTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVS 582
Cdd:cd05968 447 WPGMTRGFWRDEDRYLETYWSRFDNVWVHGDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIG 526
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 583 HPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQiAKNDRDLGDTS 662
Cdd:cd05968 527 VPHPVKGEAIVCFVVLKPGVTPTEALAEELMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIRA-AYLGKELGDLS 605
|
....
gi 1394780554 663 TVAD 666
Cdd:cd05968 606 SLEN 609
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
25-666 |
4.68e-175 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 511.36 E-value: 4.68e-175
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 25 LAQYEKLYRrsveephEF-WGDIAKEFywkshhsgpflqynfdvtkgkifvEWMKGSTTNICYNLLDRNVNEKkLGDKIA 103
Cdd:PRK04319 15 LKDYEETYA-------TFsWEEVEKEF------------------------SWLETGKVNIAYEAIDRHADGG-RKDKVA 62
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 104 FYWEGNDPGisMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCER 183
Cdd:PRK04319 63 LRYLDASRK--EKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVRDR 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 184 ILDSNCSLLVTADAFYR---GDKLISLKQIaddalqkckekgflvrncIVVNHLGREElvsDGTtsqsppvkrlcqdvqm 260
Cdd:PRK04319 141 LEDSEAKVLITTPALLErkpADDLPSLKHV------------------LLVGEDVEEG---PGT---------------- 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 261 pwnsdVDLWwhELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHtVGGYMIFTAATFKYVFDYHPEDIYWCTGDI 340
Cdd:PRK04319 184 -----LDFN--ALMEQASDEFDIEWTDREDGAILHYTSGSTGKPKGVLH-VHNAMLQHYQTGKYVLDLHEDDVYWCTADP 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 341 GWITGHSYLTYGPLANGATSVLFEGvptYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVG 420
Cdd:PRK04319 256 GWVTGTSYGIFAPWLNGATNVIDGG---RFSPERWYRILEDYKVTVWYTAPTAIRMLMGAGDDLVKKYDLSSLRHILSVG 332
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 421 EPINPEAWLWYYHVIGderCPIVDTFWQTETGGHMLTPLPAVtAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFK 500
Cdd:PRK04319 333 EPLNPEVVRWGMKVFG---LPIHDNWWMTETGGIMIANYPAM-DIKPGSMGKPLPGIEAAIVDDQGNELPPNRMGNLAIK 408
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 501 QPWPGIMRTVYGNYKRFETtYFKkfPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAV 580
Cdd:PRK04319 409 KGWPSMMRGIWNNPEKYES-YFA--GDWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGV 485
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 581 VSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQiakndRDL-- 658
Cdd:PRK04319 486 IGKPDPVRGEIIKAFVALRPGYEPSEELKEEIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRRVLKA-----WELgl 560
|
650
....*....|
gi 1394780554 659 --GDTSTVAD 666
Cdd:PRK04319 561 peGDLSTMED 570
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
118-652 |
3.77e-135 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 404.58 E-value: 3.77e-135
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTADA 197
Cdd:cd05969 2 TFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTEE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 198 FYRgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvKRlcqdvqmpwnsdvdlwwhelmrna 277
Cdd:cd05969 82 LYE---------------------------------------------------RT------------------------ 86
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 278 skecepewcDSEDELFILYTSGSTGKPKGVVHtVGGYMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANG 357
Cdd:cd05969 87 ---------DPEDPTLLHYTSGTTGTPKGVLH-VHDAMIFYYFTGKYVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNG 156
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 358 ATSVLFEGvptYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEPINPEAWLWYYHVIGd 437
Cdd:cd05969 157 VTNVVYEG---RFDAESWYGIIERVKVTVWYTAPTAIRMLMKEGDELARKYDLSSLRFIHSVGEPLNPEAIRWGMEVFG- 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 438 erCPIVDTFWQTETGGHMLTPLPAVTAmKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPWPGIMRTVYGNYKRF 517
Cdd:cd05969 233 --VPIHDTWWQTETGSIMIANYPCMPI-KPGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPGWPSMFRGIWNDEERY 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 518 ETtYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVT 597
Cdd:cd05969 310 KN-SFID--GWYLTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFIS 386
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 1394780554 598 LRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIA 652
Cdd:cd05969 387 LKEGFEPSDELKEEIINFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVLKAKE 441
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
118-649 |
6.87e-105 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 325.83 E-value: 6.87e-105
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVtada 197
Cdd:cd05972 2 SFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIV---- 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 198 fyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqdvqmpwnsdvdlwwhelmrna 277
Cdd:cd05972 --------------------------------------------------------------------------------
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 278 skecepewCDSEDELFILYTSGSTGKPKGVVHTVGgYMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANG 357
Cdd:cd05972 78 --------TDAEDPALIYFTSGTTGLPKGVLHTHS-YPLGHIPTAAYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLG 148
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 358 ATSVLFEGVPTypDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKkcsRESLKILGTVGEPINPEAWLWYYHVIGd 437
Cdd:cd05972 149 ATVFVYEGPRF--DAERILELLERYGVTSFCGPPTAYRMLIKQDLSSYK---FSHLRLVVSAGEPLNPEVIEWWRAATG- 222
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 438 erCPIVDTFWQTETGgHMLTPLPAvTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPWPGIMRTVYGNYKRF 517
Cdd:cd05972 223 --LPIRDGYGQTETG-LTVGNFPD-MPVKPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKLPPPGLFLGYVGDPEKT 298
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 518 ETTYFKkfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVT 597
Cdd:cd05972 299 EASIRG---DYYLTGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVV 375
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 1394780554 598 LRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:cd05972 376 LTSGYEPSEELAEELQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVELR 427
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
89-556 |
1.15e-104 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 324.65 E-value: 1.15e-104
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 89 LDRNVneKKLGDKIAFyweGNDPGISmsITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALH 168
Cdd:pfam00501 1 LERQA--ARTPDKTAL---EVGEGRR--LTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVY 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 169 SIVFAGFSAESLCERILDSNCSLLVTADAFYrgdklislkqiaDDALQKCKEKGFLVRNCIVVNHLGREELvsdgttsqs 248
Cdd:pfam00501 74 VPLNPRLPAEELAYILEDSGAKVLITDDALK------------LEELLEALGKLEVVKLVLVLDRDPVLKE--------- 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 249 ppvkrlcqdvqmpwnsdvDLWWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVgGYMIFTAATFKYV--- 325
Cdd:pfam00501 133 ------------------EPLPEEAKPADVPPPPPPPPDPDDLAYIIYTSGTTGKPKGVMLTH-RNLVANVLSIKRVrpr 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 326 -FDYHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLFEGVPTyPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEP 404
Cdd:pfam00501 194 gFGLGPDDRVLSTLPLFHDFGLSLGLLGPLLAGATVVLPPGFPA-LDPAALLELIERYKVTVLYGVPTLLNMLLEAGAPK 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 405 VKKCSreSLKILGTVGEPINPEAWLWYYHVIGderCPIVDTFWQTETGGHMLTPLPAVTAM-KPGSATFPFFGVVPAILD 483
Cdd:pfam00501 273 RALLS--SLRLVLSGGAPLPPELARRFRELFG---GALVNGYGLTETTGVVTTPLPLDEDLrSLGSVGRPLPGTEVKIVD 347
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1394780554 484 ES-GQELEGEAEGFLVFKQpwPGIMRTVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVS 556
Cdd:pfam00501 348 DEtGEPVPPGEPGELCVRG--PGVMKGYLNDPELTAEAFDED--GWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
88-656 |
9.34e-92 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 292.48 E-value: 9.34e-92
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 88 LLDRNVneKKLGDKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGAL 167
Cdd:COG0318 4 LLRRAA--ARHPDRPALVFGGR------RLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAV 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 168 HSIVFAGFSAESLcERIL-DSNCSLLVTAdafyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgtts 246
Cdd:COG0318 76 VVPLNPRLTAEEL-AYILeDSGARALVTA--------------------------------------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 247 qsppvkrlcqdvqmpwnsdvdlwwhelmrnaskecepewcdsedelFILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVF 326
Cdd:COG0318 104 ----------------------------------------------LILYTSGTTGRPKGVMLTHRN-LLANAAAIAAAL 136
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 327 DYHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLfegvPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVK 406
Cdd:COG0318 137 GLTPGDVVLVALPLFHVFGLTVGLLAPLLAGATLVL----LPRFDPERVLELIERERVTVLFGVPTMLARLLRHPEFARY 212
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 407 KCSreSLKILGTVGEPINPEAWLWYYHVIGderCPIVDTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESG 486
Cdd:COG0318 213 DLS--SLRLVVSGGAPLPPELLERFEERFG---VRIVEGYGLTETSPVVTVNPEDPGERRPGSVGRPLPGVEVRIVDEDG 287
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 487 QELEGEAEGFLVFKQPWpgIMRtvyGNYKRFETTyFKKFP-GYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEV 565
Cdd:COG0318 288 RELPPGEVGEIVVRGPN--VMK---GYWNDPEAT-AEAFRdGWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEV 361
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 566 ESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILR 645
Cdd:COG0318 362 EEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVLRPGAELDA---EELRAFLRERLARYKVPRRVEFVDELPRTASGKIDR 438
|
570
....*....|.
gi 1394780554 646 RVLRQIAKNDR 656
Cdd:COG0318 439 RALRERYAAGA 449
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
290-644 |
3.53e-90 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 284.18 E-value: 3.53e-90
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 290 DELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYvFDYHPEDIYWCTGDIGWItGHSYLTYGPLANGATSVLFEGvpty 369
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAAS-GGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLPK---- 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 370 PDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSreSLKILGTVGEPINPEAWLWYYHVIGderCPIVDTFWQT 449
Cdd:cd04433 75 FDPEAALELIEREKVTILLGVPTLLARLLKAPESAGYDLS--SLRALVSGGAPLPPELLERFEEAPG---IKLVNGYGLT 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 450 ETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPWPgiMRtvyGNYKRFETTYFKKFPGYY 529
Cdd:cd04433 150 ETGGTVATGPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSV--MK---GYWNNPEATAAVDEDGWY 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 530 VTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKtlq 609
Cdd:cd04433 225 RTGDLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADLDA--- 301
|
330 340 350
....*....|....*....|....*....|....*
gi 1394780554 610 EELKKQVREKIGPIATPDYIQHAPGLPKTRSGKIL 644
Cdd:cd04433 302 EELRAHVRERLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
14-651 |
5.97e-87 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 285.32 E-value: 5.97e-87
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 14 AALAREAHVSSLAQYEKLYRRSVEEPHEFWGDIAKEFYWKSHHsgpflQYNFDVTKGKIF--VEWMKGSTTNICYNLLDR 91
Cdd:cd05943 5 RRWVNARHGLSLADYAALHRWSVDDPGAFWAAVWDFSGVRGSK-----PYDVVVVSGRIMpgARWFPGARLNYAENLLRH 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 92 NVNEkklgDKIAFYweGNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIV 171
Cdd:cd05943 80 ADAD----DPAAIY--AAEDGERTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSC 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 172 FAGFSAESLCERILDSNCSLLVTADAFYRGDKLIslkqiadDALQKckekgflvrncivVNHLgREELvsdGTTSQSPPV 251
Cdd:cd05943 154 SPDFGVPGVLDRFGQIEPKVLFAVDAYTYNGKRH-------DVREK-------------VAEL-VKGL---PSLLAVVVV 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 252 KRLCQDVQMPW-NSDVDLWWHE-LMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYH 329
Cdd:cd05943 210 PYTVAAGQPDLsKIAKALTLEDfLATGAAGELEFEPLPFDHPLYILYSSGTTGLPKCIVHGAGGTLLQHLKEHILHCDLR 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 330 PEDIYWCTGDIGWITGHsYLTYGpLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCS 409
Cdd:cd05943 290 PGDRLFYYTTCGWMMWN-WLVSG-LAVGATIVLYDGSPFYPDTNALWDLADEEGITVFGTSAKYLDALEKAGLKPAETHD 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 410 RESLKILGTVGEPINPEAWLWYYHVIGDercpivDTFWQTETGG------HMLT-PLPAVTamkPGSATFPFFGVVPAIL 482
Cdd:cd05943 368 LSSLRTILSTGSPLKPESFDYVYDHIKP------DVLLASISGGtdiiscFVGGnPLLPVY---RGEIQCRGLGMAVEAF 438
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 483 DESGQELEGEAeGFLVFKQPWPGImrTVY----GNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGH 558
Cdd:cd05943 439 DEEGKPVWGEK-GELVCTKPFPSM--PVGfwndPDGSRYRAAYFAKYPGVWAHGDWIEITPRGGVVILGRSDGTLNPGGV 515
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 559 LLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKT 638
Cdd:cd05943 516 RIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDERVILFVKLREGVELDDELRKRIRSTIRSALSPRHVPAKIIAVPDIPRT 595
|
650
....*....|...
gi 1394780554 639 RSGKILRRVLRQI 651
Cdd:cd05943 596 LSGKKVEVAVKKI 608
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
28-648 |
5.32e-79 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 264.68 E-value: 5.32e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 28 YEKLYRRSVEEPHEFWGDIAKEF-YWKShhsgpflQYNFDVTKGKIFVEWMKGSTTNICYNLLDRNVNEKKLGDKIAFYW 106
Cdd:PTZ00237 10 YENDSNYANSNPESFWDEVAKKYvHWDK-------MYDKVYSGDEIYPDWFKGGELNTCYNVLDIHVKNPLKRDQDALIY 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 107 EGNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILD 186
Cdd:PTZ00237 83 ECPYLKKTIKLTYYQLYEKVCEFSRVLLNLNISKNDNVLIYMANTLEPLIAMLSCARIGATHCVLFDGYSVKSLIDRIET 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 187 SNCSLLVTADAFYRGDKLISLKQIADDALQKCKekgFLVRNCIVvnhLGREELVSDGTtsqsppvKRLCQDVQMPWNSdV 266
Cdd:PTZ00237 163 ITPKLIITTNYGILNDEIITFTPNLKEAIELST---FKPSNVIT---LFRNDITSESD-------LKKIETIPTIPNT-L 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 267 DlWWHELMR----NASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPEDIYWCTGDIGW 342
Cdd:PTZ00237 229 S-WYDEIKKikenNQSPFYEYVPVESSHPLYILYTSGTTGNSKAVVRSNGPHLVGLKYYWRSIIEKDIPTVVFSHSSIGW 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 343 ITGHSYLtYGPLANGATSVLFEGVPTYPDVSR--MWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSR---ESLKILG 417
Cdd:PTZ00237 308 VSFHGFL-YGSLSLGNTFVMFEGGIIKNKHIEddLWNTIEKHKVTHTLTLPKTIRYLIKTDPEATIIRSKydlSNLKEIW 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 418 TVGEPIN---PEawlwyyHVIGDERCPIVDTFWQTETGGHMLTPLPAVTamKPGSAT-FPFFGVVPAILDESGQELEGEA 493
Cdd:PTZ00237 387 CGGEVIEesiPE------YIENKLKIKSSRGYGQTEIGITYLYCYGHIN--IPYNATgVPSIFIKPSILSEDGKELNVNE 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 494 EGFLVFKQPWP-GIMRTVYGNYKRFETTyFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEH 572
Cdd:PTZ00237 459 IGEVAFKLPMPpSFATTFYKNDEKFKQL-FSKFPGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKH 537
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 573 KAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKT----LQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:PTZ00237 538 PLVLECCSIGIYDPDCYNVPIGLLVLKQDQSNQSIdlnkLKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPRQII 617
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
117-650 |
1.18e-76 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 252.44 E-value: 1.18e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTad 196
Cdd:cd05973 1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVT-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 afyrgdklislkqiadDALQKckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqdvqmpwnsdvdlwwHELmrn 276
Cdd:cd05973 79 ----------------DAANR-----------------------------------------------------HKL--- 86
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 askecepewcdSEDELFILYTSGSTGKPKGVVHTVGGYMIFtAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLAN 356
Cdd:cd05973 87 -----------DSDPFVMMFTSGTTGLPKGVPVPLRALAAF-GAYLRDAVDLRPEDSFWNAADPGWAYGLYYAITGPLAL 154
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 357 GATSVLFEGVPTYPDVsrmWNIVDKYKVTKLYTSPTAIRALMKSGdEPVKKCSRESLKILGTVGEPINPEAWLWYYHVIG 436
Cdd:cd05973 155 GHPTILLEGGFSVEST---WRVIERLGVTNLAGSPTAYRLLMAAG-AEVPARPKGRLRRVSSAGEPLTPEVIRWFDAALG 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 437 derCPIVDTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPWPGIMrtVYGNYKR 516
Cdd:cd05973 231 ---VPIHDHYGQTELGMVLANHHALEHPVHAGSAGRAMPGWRVAVLDDDGDELGPGEPGRLAIDIANSPLM--WFRGYQL 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 517 FETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFV 596
Cdd:cd05973 306 PDTPAIDG--GYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFV 383
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 1394780554 597 TLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:cd05973 384 VLRGGHEGTPALADELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLRR 437
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
17-648 |
8.43e-74 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 250.87 E-value: 8.43e-74
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 17 AREAHVSSLAQYEKLYRRSVEEPHEFWGDIAKEFYWKSHHSGPFLQYNfdvtKGKIFVEWMKGSTTNICYNLLdRNvnek 96
Cdd:PRK03584 25 LAARRGLSFDDYAALWRWSVEDLEAFWQSVWDFFGVIGSTPYTVVLAG----RRMPGARWFPGARLNYAENLL-RH---- 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 97 KLGDKIAFYWEGNDpGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFS 176
Cdd:PRK03584 96 RRDDRPAIIFRGED-GPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFG 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 177 AESLCERILDSNCSLLVTADA-FYRG---DKLISLKQIAdDALQKckekgflVRNCIVVNHLGreelvsdgttsqSPPVK 252
Cdd:PRK03584 175 VQGVLDRFGQIEPKVLIAVDGyRYGGkafDRRAKVAELR-AALPS-------LEHVVVVPYLG------------PAAAA 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 253 RLCQDVQMpwnsdvdlwWHELMRNAS-KECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPE 331
Cdd:PRK03584 235 AALPGALL---------WEDFLAPAEaAELEFEPVPFDHPLWILYSSGTTGLPKCIVHGHGGILLEHLKELGLHCDLGPG 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 332 D-IYWCT--GDIGWitghSYLTYGpLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKC 408
Cdd:PRK03584 306 DrFFWYTtcGWMMW----NWLVSG-LLVGATLVLYDGSPFYPDPNVLWDLAAEEGVTVFGTSAKYLDACEKAGLVPGETH 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 409 SRESLKILGTVGEPINPEAWLWYYHVIGDercpivDTFWQTETGGhmlT----------PLPAVTamkPGSATFPFFGVV 478
Cdd:PRK03584 381 DLSALRTIGSTGSPLPPEGFDWVYEHVKA------DVWLASISGG---TdicscfvggnPLLPVY---RGEIQCRGLGMA 448
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 479 PAILDESGQELEGEAeGFLVFKQPWPGiMrTVY----GNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLN 554
Cdd:PRK03584 449 VEAWDEDGRPVVGEV-GELVCTKPFPS-M-PLGfwndPDGSRYRDAYFDTFPGVWRHGDWIEITEHGGVVIYGRSDATLN 525
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 555 VSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPG 634
Cdd:PRK03584 526 RGGVRIGTAEIYRQVEALPEVLDSLVIGQEWPDGDVRMPLFVVLAEGVTLDDALRARIRTTIRTNLSPRHVPDKIIAVPD 605
|
650
....*....|....*...
gi 1394780554 635 LPKTRSGKIL----RRVL 648
Cdd:PRK03584 606 IPRTLSGKKVelpvKKLL 623
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
112-650 |
4.49e-65 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 221.54 E-value: 4.49e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 112 GISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSL 191
Cdd:cd05971 2 GTPEKVTFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASA 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 192 LVTadafyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsDGttsqsppvkrlcqdvqmpwnsdvdlwwh 271
Cdd:cd05971 82 LVT-----------------------------------------------DG---------------------------- 86
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 272 elmrnaskecepewcdSEDELFILYTSGSTGKPKGVVHT--------VGGYMIFTAATfkyvfdyHPEDIYWCTGDIGWI 343
Cdd:cd05971 87 ----------------SDDPALIIYTSGTTGPPKGALHAhrvllghlPGVQFPFNLFP-------RDGDLYWTPADWAWI 143
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 344 tghsyltyGPLANGATSVLFEGVP------TYPDVSRMWNIVDKYKVTKLYTSPTAIRaLMKSGDEPVKKCSREsLKILG 417
Cdd:cd05971 144 --------GGLLDVLLPSLYFGVPvlahrmTKFDPKAALDLMSRYGVTTAFLPPTALK-MMRQQGEQLKHAQVK-LRAIA 213
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 418 TVGEPINPEAWLWYYHVIGDErcpIVDTFWQTEtGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFL 497
Cdd:cd05971 214 TGGESLGEELLGWAREQFGVE---VNEFYGQTE-CNLVIGNCSALFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEI 289
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 498 VFKQPWPGIMRTVYGNYKRFEttyfKKFPG-YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAIS 576
Cdd:cd05971 290 AVELPDPVAFLGYWNNPSATE----KKMAGdWLLTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVL 365
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1394780554 577 EAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:cd05971 366 MAAVVGIPDPIRGEIVKAFVVLNPGETPSDALAREIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELRA 439
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
86-649 |
1.29e-63 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 219.55 E-value: 1.29e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 86 YN---LLDRNVNEKkLGDKIAFYwegnDPgiSMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACA 162
Cdd:cd05959 3 YNaatLVDLNLNEG-RGDKTAFI----DD--AGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAI 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 163 RIGALHSIVFAGFSAESLCERILDSNCSLLVTADAFYrgdklislkQIADDALQKckekgflvrncivVNHLGREELVSD 242
Cdd:cd05959 76 RAGIVPVPVNTLLTPDDYAYYLEDSRARVVVVSGELA---------PVLAAALTK-------------SEHTLVVLIVSG 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 243 GTTSQSPpvkrlcqdvqmpwnsdvDLWWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGyMIFTAATF 322
Cdd:cd05959 134 GAGPEAG-----------------ALLLAELVAAEAEQLKPAATHADDPAFWLYSSGSTGRPKGVVHLHAD-IYWTAELY 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 323 -KYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLFEGVPTyPDvsRMWNIVDKYKVTKLYTSPTAIRALMKSg 401
Cdd:cd05959 196 aRNVLGIREDDVCFSAAKLFFAYGLGNSLTFPLSVGATTVLMPERPT-PA--AVFKRIRRYRPTVFFGVPTLYAAMLAA- 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 402 dEPVKKCSRESLKILGTVGEPINPEAWLWYYHVIGderCPIVDTFWQTETGGHMLTPLPAvtAMKPGSATFPFFGVVPAI 481
Cdd:cd05959 272 -PNLPSRDLSSLRLCVSAGEALPAEVGERWKARFG---LDILDGIGSTEMLHIFLSNRPG--RVRYGTTGKPVPGYEVEL 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 482 LDESGQELEGEAEGFLVFKQPWPGIMrtVYGNYKRFETTyfkkFPGYYV-TGDGCRRDKDGYYWITGRIDDMLNVSGHLL 560
Cdd:cd05959 346 RDEDGGDVADGEPGELYVRGPSSATM--YWNNRDKTRDT----FQGEWTrTGDKYVRDDDGFYTYAGRADDMLKVSGIWV 419
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 561 STAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRS 640
Cdd:cd05959 420 SPFEVESALVQHPAVLEAAVVGVEDEDGLTKPKAFVVLRPGYEDSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTAT 499
|
....*....
gi 1394780554 641 GKILRRVLR 649
Cdd:cd05959 500 GKIQRFKLR 508
|
|
| ac_ac_CoA_syn |
TIGR01217 |
acetoacetyl-CoA synthase; This enzyme catalyzes the first step of the mevalonate pathway of ... |
15-651 |
6.24e-62 |
|
acetoacetyl-CoA synthase; This enzyme catalyzes the first step of the mevalonate pathway of IPP biosynthesis. Most bacteria do not use this pathway, but rather the deoxyxylulose pathway. [Central intermediary metabolism, Other]
Pssm-ID: 273507 [Multi-domain] Cd Length: 652 Bit Score: 218.60 E-value: 6.24e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 15 ALAREAHVSSLAQYEKLYRRSVEEPHEFWGDIAKEFywKSHHSGPFLQYnFDVTKGkIFVEWMKGSTTNICYNLLdrnvn 94
Cdd:TIGR01217 24 AWAGEHHGAAEGGYDALHRWSVDELDTFWKAVWEWF--DVRFSTPCARV-VDDRTM-PGAQWFPGARLNYAENLL----- 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 95 eKKLGDKIAFYWEGNDPgISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAG 174
Cdd:TIGR01217 95 -RAAGTEPALLYVDETH-EPAPVTWAELRRQVASLAAALRALGVRPGDRVSGYLPNIPQAVVAMLATASVGAIWSSCSPD 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 175 FSAESLCERILDSNCSLLVTADAFYRGDKlislKQIADDALQKCKEKGFLVRNCIVVNHLGREElvsdgttSQSPPVkrl 254
Cdd:TIGR01217 173 FGARGVLDRFQQIEPKLLFTVDGYRYNGK----EHDRRDKVAEVRKELPTLRAVVHIPYLGPRE-------TEAPKI--- 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 255 cqdvqmpwnsDVDLWWHELMRNA-SKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPEDI 333
Cdd:TIGR01217 239 ----------DGALDLEDFTAAAqAAELVFEQLPFDHPLWILFSSGTTGLPKCIVHSAGGTLVQHLKEHGLHCDLGPGDR 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 334 YWCTGDIGWITgHSYLTYGpLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESL 413
Cdd:TIGR01217 309 LFYYTTTGWMM-WNWLVSG-LATGATLVLYDGSPGFPATNVLWDIAERTGATLFGTSAKYVMACRKAGVHPARTHDLSAL 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 414 KILGTVGEPINPEAWLWYYhvigdeRCPIVDTFWQTETGG-HMLTPLPAVTAMKP---GSATFPFFGVVPAILDESGQEL 489
Cdd:TIGR01217 387 QCVASTGSPLPPDGFRWVY------DEIKADVWLASISGGtDICSCFAGANPTLPvhiGEIQAPGLGTAVQSWDPEGKPV 460
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 490 EGEAeGFLVFKQPWPGiMRTVYGNYK---RFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVE 566
Cdd:TIGR01217 461 TGEV-GELVCTNPMPS-MPIRFWNDPdgsKYRDAYFDTYPGVWRHGDWITLTPRGGIVIHGRSDSTLNPQGVRMGSAEIY 538
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 567 SALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRR 646
Cdd:TIGR01217 539 NAVERLDEVRESLCIGQEQPDGGYRVVLFVHLAPGATLDDALLDRIKRTIRAGLSPRHVPDEIIEVPGIPHTLTGKRVEV 618
|
....*
gi 1394780554 647 VLRQI 651
Cdd:TIGR01217 619 AVKRV 623
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
86-650 |
1.57e-61 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 214.28 E-value: 1.57e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 86 YNLLDRNVneKKLGDKIAFYWEGNDpgismsITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIG 165
Cdd:PRK06187 9 GRILRHGA--RKHPDKEAVYFDGRR------TTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIG 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 166 A-LHSI-VFagFSAESLcERILDsncsllvtaDAfyrGDKLIslkqIADDA----LQKCKEKGFLVRNCIVVNHLGREEL 239
Cdd:PRK06187 81 AvLHPInIR--LKPEEI-AYILN---------DA---EDRVV----LVDSEfvplLAAILPQLPTVRTVIVEGDGPAAPL 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 240 VsdgttsqsPPVKRlcqdvqmpwnsdvdlwWHELMRNASKEcePEWCD-SEDELF-ILYTSGSTGKPKGVVHT----VGG 313
Cdd:PRK06187 142 A--------PEVGE----------------YEELLAAASDT--FDFPDiDENDAAaMLYTSGTTGHPKGVVLShrnlFLH 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 314 YMIFTAAtfkyvFDYHPEDIY-----------WctgdiGWitghsylTYGPLANGATSVlfegvptYP---DVSRMWNIV 379
Cdd:PRK06187 196 SLAVCAW-----LKLSRDDVYlvivpmfhvhaW-----GL-------PYLALMAGAKQV-------IPrrfDPENLLDLI 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 380 DKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGtvGEPINP---EAWLWYYhvigdeRCPIVDTFWQTETGG--H 454
Cdd:PRK06187 252 ETERVTFFFAVPTIWQMLLKAPRAYFVDFSSLRLVIYG--GAALPPallREFKEKF------GIDLVQGYGMTETSPvvS 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 455 MLTPLPAVTAM--KPGSATFPFFGVVPAILDESGQELE--GEAEGFLVFKQPWpgIMRtvyGNYKRFETTYfKKFP-GYY 529
Cdd:PRK06187 324 VLPPEDQLPGQwtKRRSAGRPLPGVEARIVDDDGDELPpdGGEVGEIIVRGPW--LMQ---GYWNRPEATA-ETIDgGWL 397
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 530 VTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQ 609
Cdd:PRK06187 398 HTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATLD---A 474
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 1394780554 610 EELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:PRK06187 475 KELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVLRE 515
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
83-649 |
2.69e-60 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 211.17 E-value: 2.69e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 83 NICYNLLDRNVNEKKLGDK---IAFYWEgNDPGISMSITYRQLLQDVCQFASVLQEQ-GIKKGDRISIYMPMIIELVVAM 158
Cdd:cd05928 6 NFASDVLDQWADKEKAGKRppnPALWWV-NGKGDEVKWSFRELGSLSRKAANVLSGAcGLQRGDRVAVILPRVPEWWLVN 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 159 LACARIGAlhsivfagfsaeslcerILDSNCSLLVTADAFYRgdklislkqiaddaLQKCKEKgflvrnCIVVNhlgrEE 238
Cdd:cd05928 85 VACIRTGL-----------------VFIPGTIQLTAKDILYR--------------LQASKAK------CIVTS----DE 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 239 LVS--DGTTSQSPPVKRLCQDVQMPWNSDVDLwwHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMI 316
Cdd:cd05928 124 LAPevDSVASECPSLKTKLLVSEKSRDGWLNF--KELLNEASTEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGL 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 317 FTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANGATsVLFEGVPTYpDVSRMWNIVDKYKVTKLYTSPTAIRA 396
Cdd:cd05928 202 GLKVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEPWIQGAC-VFVHHLPRF-DPLVILKTLSSYPITTFCGAPTVYRM 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 397 LMKsgdEPVKKCSRESLKILGTVGEPINPEAWLWYYHVIGDErcpIVDTFWQTETGghMLTPLPAVTAMKPGSATFPFFG 476
Cdd:cd05928 280 LVQ---QDLSSYKFPSLQHCVTGGEPLNPEVLEKWKAQTGLD---IYEGYGQTETG--LICANFKGMKIKPGSMGKASPP 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 477 VVPAILDESGQELEGEAEGFLVFK----QPWpGIMRTVYGNYKRFETTYFKKFpgyYVTGDGCRRDKDGYYWITGRIDDM 552
Cdd:cd05928 352 YDVQIIDDNGNVLPPGTEGDIGIRvkpiRPF-GLFSGYVDNPEKTAATIRGDF---YLTGDRGIMDEDGYFWFMGRADDV 427
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 553 LNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTL------RDGHEFSKTLQEELKKQVrekiGPIATP 626
Cdd:cd05928 428 INSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVVKAFVVLapqflsHDPEQLTKELQQHVKSVT----APYKYP 503
|
570 580
....*....|....*....|...
gi 1394780554 627 DYIQHAPGLPKTRSGKILRRVLR 649
Cdd:cd05928 504 RKVEFVQELPKTVTGKIQRNELR 526
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
86-649 |
1.26e-59 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 207.80 E-value: 1.26e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 86 YNLLDRNVneKKLGDKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIG 165
Cdd:cd05936 2 ADLLEEAA--RRFPDKTALIFMGR------KLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAG 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 166 AlhsIVFagfsaeslcerildsNCSLLVTADafyrgdklislkqiaddalqkckEKGFLVRNCivvnhlGREELVsdgtt 245
Cdd:cd05936 74 A---VVV---------------PLNPLYTPR-----------------------ELEHILNDS------GAKALI----- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 246 sqsppvkrlcqdvqmpwnsdVDLWWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGG-YMIFTAATFKY 324
Cdd:cd05936 102 --------------------VAVSFTDLLAAGAPLGERVALTPEDVAVLQYTSGTTGVPKGAMLTHRNlVANALQIKAWL 161
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 325 VFDYHPEDIYWCTGDIgwitGHSY-LTYG---PLANGATSVLfegVPTyPDVSRMWNIVDKYKVTKLYTSPTAIRALMKS 400
Cdd:cd05936 162 EDLLEGDDVVLAALPL----FHVFgLTVAlllPLALGATIVL---IPR-FRPIGVLKEIRKHRVTIFPGVPTMYIALLNA 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 401 GDepVKKCSRESLKILGTVGEPINPEawlwyyhvigdercpiVDTFWQTETG-----GHMLT---PLPAVTAM----KPG 468
Cdd:cd05936 234 PE--FKKRDFSSLRLCISGGAPLPVE----------------VAERFEELTGvpiveGYGLTetsPVVAVNPLdgprKPG 295
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 469 SATFPFFGVVPAILDESGQELEGEAEGFLVFKQpwPGIMRtvyGNYKRFETT--YFKKfpGYYVTGDGCRRDKDGYYWIT 546
Cdd:cd05936 296 SIGIPLPGTEVKIVDDDGEELPPGEVGELWVRG--PQVMK---GYWNRPEETaeAFVD--GWLRTGDIGYMDEDGYFFIV 368
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 547 GRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATP 626
Cdd:cd05936 369 DRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFVVLKEGASLTE---EEIIAFCREQLAGYKVP 445
|
570 580
....*....|....*....|...
gi 1394780554 627 DYIQHAPGLPKTRSGKILRRVLR 649
Cdd:cd05936 446 RQVEFRDELPKSAVGKILRRELR 468
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
99-645 |
2.27e-58 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 203.23 E-value: 2.27e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 99 GDKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAE 178
Cdd:cd17631 9 PDRTALVFGGR------SLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPP 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 179 SLCERILDSNCSLLVtadafyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqdv 258
Cdd:cd17631 83 EVAYILADSGAKVLF----------------------------------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 259 qmpwnsdvdlwwhelmrnaskecepewcdsEDELFILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTG 338
Cdd:cd17631 98 ------------------------------DDLALLMYTSGTTGRPKGAMLTHRN-LLWNAVNALAALDLGPDDVLLVVA 146
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 339 DIGWITGHSYLTYGPLANGATSVLFEGvptyPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSreSLKILGT 418
Cdd:cd17631 147 PLFHIGGLGVFTLPTLLRGGTVVILRK----FDPETVLDLIERHRVTSFFLVPTMIQALLQHPRFATTDLS--SLRAVIY 220
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 419 VGEPInPEAWLWYYHVIGderCPIVDTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLV 498
Cdd:cd17631 221 GGAPM-PERLLRALQARG---VKFVQGYGMTETSPGVTFLSPEDHRRKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIV 296
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 499 FKQPwpGIMRtvyGNYKRFETT--YFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAIS 576
Cdd:cd17631 297 VRGP--HVMA---GYWNRPEATaaAFRD--GWFHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVA 369
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1394780554 577 EAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILR 645
Cdd:cd17631 370 EVAVIGVPDEKWGEAVVAVVVPRPGAELD---EDELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
83-650 |
5.23e-57 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 202.34 E-value: 5.23e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 83 NICYNLLDRNVNEKKlgDKIAFYWeGNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACA 162
Cdd:cd05970 17 NFAYDVVDAMAKEYP--DKLALVW-CDDAGEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALH 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 163 RIGALHSIVFAGFSAESLCERILDSNCSLLVTADAfyrgdklISLKQIADDALQKCKekgflvrncivvnhlgreelvsd 242
Cdd:cd05970 94 KLGAIAIPATHQLTAKDIVYRIESADIKMIVAIAE-------DNIPEEIEKAAPECP----------------------- 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 243 gttsqSPPVKRLCQDvqmpwnSDVDLW--WHELMRNASKECE-PEWCDS---EDELFILYTSGSTGKPKGVVHtVGGYMI 316
Cdd:cd05970 144 -----SKPKLVWVGD------PVPEGWidFRKLIKNASPDFErPTANSYpcgEDILLVYFSSGTTGMPKMVEH-DFTYPL 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 317 FTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLFEGVPTYPDvsRMWNIVDKYKVTKLYTSPTAIRA 396
Cdd:cd05970 212 GHIVTAKYWQNVREGGLHLTVADTGWGKAVWGKIYGQWIAGAAVFVYDYDKFDPK--ALLEKLSKYGVTTFCAPPTIYRF 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 397 LMKsgdEPVKKCSRESLKILGTVGEPINPEAWLWYYHVIGDErcpIVDTFWQTETGGHMLTpLPAVTAmKPGSATFPFFG 476
Cdd:cd05970 290 LIR---EDLSRYDLSSLRYCTTAGEALNPEVFNTFKEKTGIK---LMEGFGQTETTLTIAT-FPWMEP-KPGSMGKPAPG 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 477 VVPAILDESGQELEGEAEGFLVF----KQPWpGIMRTVYGNYKRFETTYFKkfpGYYVTGDGCRRDKDGYYWITGRIDDM 552
Cdd:cd05970 362 YEIDLIDREGRSCEAGEEGEIVIrtskGKPV-GLFGGYYKDAEKTAEVWHD---GYYHTGDAAWMDEDGYLWFVGRTDDL 437
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 553 LNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHA 632
Cdd:cd05970 438 IKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQVVKATIVLAKGYEPSEELKKELQDHVKKVTAPYKYPRIVEFV 517
|
570
....*....|....*...
gi 1394780554 633 PGLPKTRSGKILRRVLRQ 650
Cdd:cd05970 518 DELPKTISGKIRRVEIRE 535
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
116-649 |
1.35e-56 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 198.45 E-value: 1.35e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTa 195
Cdd:cd05919 10 SVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARLVVT- 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 dafyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqdvqmpwnsdvdlwwhelmr 275
Cdd:cd05919 --------------------------------------------------------------------------------
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 naskecepewcDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPEDIYWCTGDI--GWITGHSylTYGP 353
Cdd:cd05919 89 -----------SADDIAYLLYSSGTTGPPKGVMHAHRDPLLFADAMAREALGLTPGDRVFSSAKMffGYGLGNS--LWFP 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 354 LANGATSVLFegvPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSreSLKILGTVGEPINPEAWLWYYH 433
Cdd:cd05919 156 LAVGASAVLN---PGWPTAERVLATLARFRPTVLYGVPTFYANLLDSCAGSPDALR--SLRLCVSAGEALPRGLGERWME 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 434 VIGderCPIVDTFWQTETGGHMLTPLPAvtAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPWPGImrtvyGN 513
Cdd:cd05919 231 HFG---GPILDGIGATEVGHIFLSNRPG--AWRLGSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLVRGPSAAV-----GY 300
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 514 YKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLY 593
Cdd:cd05919 301 WNNPEKSRATFNGGWYRTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLT 380
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 1394780554 594 CFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:cd05919 381 AFVVLKSPAAPQESLARDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
100-653 |
6.13e-54 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 193.61 E-value: 6.13e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGndpgisMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAES 179
Cdd:PRK08316 26 DKTALVFGD------RSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 180 LCERILDSNCSLLVTADAFYrgdklislkQIADDALQKCKEKGFLVRncivvnHLGREELVSDGTTsqsppvkrlcqdvq 259
Cdd:PRK08316 100 LAYILDHSGARAFLVDPALA---------PTAEAALALLPVDTLILS------LVLGGREAPGGWL-------------- 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 260 mpwnsDVDLWwheLMRNASKECEPEwCDSEDELFILYTSGSTGKPKGVVHT----VGGYMIFTAATfkyvfDYHPEDI-- 333
Cdd:PRK08316 151 -----DFADW---AEAGSVAEPDVE-LADDDLAQILYTSGTESLPKGAMLThralIAEYVSCIVAG-----DMSADDIpl 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 334 -----YWCTGDigwitgHSYLtyGP-LANGATSVLFEGvptyPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGD----- 402
Cdd:PRK08316 217 halplYHCAQL------DVFL--GPyLYVGATNVILDA----PDPELILRTIEAERITSFFAPPTVWISLLRHPDfdtrd 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 403 ---------------EPVKKCSRESLkilgtvgepinPEAWLWYYhvigdercpivdtFWQTETGghmltPLPAV----- 462
Cdd:PRK08316 285 lsslrkgyygasimpVEVLKELRERL-----------PGLRFYNC-------------YGQTEIA-----PLATVlgpee 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 463 TAMKPGSATFPFFGVVPAILDESGQEL-EGEAeGFLVFKQPwpgimRTVYGNYKRFETT--YFKKfpGYYVTGDGCRRDK 539
Cdd:PRK08316 336 HLRRPGSAGRPVLNVETRVVDDDGNDVaPGEV-GEIVHRSP-----QLMLGYWDDPEKTaeAFRG--GWFHSGDLGVMDE 407
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 540 DGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREK 619
Cdd:PRK08316 408 EGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATVTE---DELIAHCRAR 484
|
570 580 590
....*....|....*....|....*....|....
gi 1394780554 620 IGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAK 653
Cdd:PRK08316 485 LAGFKVPKRVIFVDELPRNPSGKILKRELRERYA 518
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
117-649 |
1.60e-51 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 184.42 E-value: 1.60e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALhsivfagfsaeslcerildsncslLVTAD 196
Cdd:cd05934 4 WTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAV------------------------LVPIN 59
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 AFYRGDKLislKQIADDAlqkckekgflvRNCIVVnhlgreelvsdgttsqsppvkrlcqdvqmpwnsdVDLwwhelmrn 276
Cdd:cd05934 60 TALRGDEL---AYIIDHS-----------GAQLVV----------------------------------VDP-------- 83
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 askeCEpewcdsedelfILYTSGSTGKPKGVV--HTvggYMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPL 354
Cdd:cd05934 84 ----AS-----------ILYTSGTTGPPKGVVitHA---NLTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAAL 145
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 355 ANGATSVLfegVPTYpDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPvkkcSRESLKILGTVGEPINPEawlwyYHV 434
Cdd:cd05934 146 SVGATLVL---LPRF-SASRFWSDVRRYGATVTNYLGAMLSYLLAQPPSP----DDRAHRLRAAYGAPNPPE-----LHE 212
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 435 IGDER--CPIVDTFWQTETGGHMLTPLPAVTamKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFK-QPWPGIMRtvy 511
Cdd:cd05934 213 EFEERfgVRLLEGYGMTETIVGVIGPRDEPR--RPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIRgLRGWGFFK--- 287
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 512 GNYKRFETTYfKKFP-GYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGE 590
Cdd:cd05934 288 GYYNMPEATA-EAMRnGWFHTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGED 366
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 1394780554 591 CLYCFVTLRDGhefSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:cd05934 367 EVKAVVVLRPG---ETLDPEELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
111-650 |
2.79e-51 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 185.59 E-value: 2.79e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 111 PGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLcERILDSNCS 190
Cdd:cd05926 9 PGSTPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEF-EFYLADLGS 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 191 LLVTADAFYRGDKLISLKQIADDALqkckEKGFLVRNCIVVnhlgreelVSDGTTSQSPPVKRlcqdvqmpwnsdvdlww 270
Cdd:cd05926 88 KLVLTPKGELGPASRAASKLGLAIL----ELALDVGVLIRA--------PSAESLSNLLADKK----------------- 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 271 helmrNASKECEPewcDSEDELFILYTSGSTGKPKGV--VHTvggyMIFTAAT-FKYVFDYHPEDIYWCT-------GDI 340
Cdd:cd05926 139 -----NAKSEGVP---LPDDLALILHTSGTTGRPKGVplTHR----NLAASATnITNTYKLTPDDRTLVVmplfhvhGLV 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 341 GwitghSYLTygPLANGATSVlfegVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSgDEPVKKCSRESLKILGTVG 420
Cdd:cd05926 207 A-----SLLS--TLAAGGSVV----LPPRFSASTFWPDVRDYNATWYTAVPTIHQILLNR-PEPNPESPPPKLRFIRSCS 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 421 EPINPEawlwyyHVIGDE---RCPIVDTFWQTETGGHM-LTPLPaVTAMKPGSATFPFfGVVPAILDESGQELEGEAEGF 496
Cdd:cd05926 275 ASLPPA------VLEALEatfGAPVLEAYGMTEAAHQMtSNPLP-PGPRKPGSVGKPV-GVEVRILDEDGEILPPGVVGE 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 497 LVFKQPwpGIMRTVYGNYK-RFEttYFKKFpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAI 575
Cdd:cd05926 347 ICLRGP--NVTRGYLNNPEaNAE--AAFKD-GWFRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAV 421
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1394780554 576 SEAAVVSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:cd05926 422 LEAVAFGVPDEKYGEEVAAAVVLREGASVTE---EELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
116-644 |
1.24e-49 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 180.87 E-value: 1.24e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTA 195
Cdd:cd05911 10 ELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIFTD 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 DAFYrgdklislkqiaDDALQKCKEKGFLVRncIVVnhlgreelvsdgTTSQSPPVKRLCQDVQMPWNsdVDLWWHELMR 275
Cdd:cd05911 90 PDGL------------EKVKEAAKELGPKDK--IIV------------LDDKPDGVLSIEDLLSPTLG--EEDEDLPPPL 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 NASKEcepewcdseDELFILYTSGSTGKPKGVVHTvggYMIFTAATfKYVFDYHPEDIYWCTGDIG-----WITGHSYLT 350
Cdd:cd05911 142 KDGKD---------DTAAILYSSGTTGLPKGVCLS---HRNLIANL-SQVQTFLYGNDGSNDVILGflplyHIYGLFTTL 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 351 YGPLaNGATSVLFEGvptyPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDepVKKCSRESLKILGTVGEPINPEawlw 430
Cdd:cd05911 209 ASLL-NGATVIIMPK----FDSELFLDLIEKYKITFLYLVPPIAAALAKSPL--LDKYDLSSLRVILSGGAPLSKE---- 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 431 yyhvIGDE------RCPIVDTFWQTETGGHMLTPLPavTAMKPGSATFPFFGVVPAILDESGQELEGEAE-GFLVFKQPw 503
Cdd:cd05911 278 ----LQELlakrfpNATIKQGYGMTETGGILTVNPD--GDDKPGSVGRLLPNVEAKIVDDDGKDSLGPNEpGEICVRGP- 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 504 pGIMRTVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSH 583
Cdd:cd05911 351 -QVMKGYYNNPEATKETFDED--GWLHTGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGI 427
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1394780554 584 PHPVKGECLYCFVTLRDGhefSKTLQEELKKQVREKIgpiatPDYIQHAPG------LPKTRSGKIL 644
Cdd:cd05911 428 PDEVSGELPRAYVVRKPG---EKLTEKEVKDYVAKKV-----ASYKQLRGGvvfvdeIPKSASGKIL 486
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
288-649 |
9.40e-48 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 174.20 E-value: 9.40e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 288 SEDELFILYTSGSTGKPKGVVHTVGGYmIFTAATF-KYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLFEGv 366
Cdd:cd05958 96 SDDICILAFTSGTTGAPKATMHFHRDP-LASADRYaVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEE- 173
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 367 pTYPDvsRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSreSLKILGTVGEPINPEAWLWYYHVIGderCPIVDTF 446
Cdd:cd05958 174 -ATPD--LLLSAIARYKPTVLFTAPTAYRAMLAHPDAAGPDLS--SLRKCVSAGEALPAALHRAWKEATG---IPIIDGI 245
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 447 WQTETGGHMLTPLPAvtAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPwpgimrTVY-GNYKRFETTYFKKf 525
Cdd:cd05958 246 GSTEMFHIFISARPG--DARPGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAVRGP------TGCrYLADKRQRTYVQG- 316
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 526 pGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFS 605
Cdd:cd05958 317 -GWNITGDTYSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPG 395
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 1394780554 606 KTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:cd05958 396 PVLARELQDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
83-650 |
2.20e-47 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 175.03 E-value: 2.20e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 83 NICYNLLDRNVNEKKlGDKIAFywegNDPgiSMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACA 162
Cdd:TIGR02262 4 NAAEDLLDRNVVEGR-GGKTAF----IDD--ISSLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAI 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 163 RIGALHSIVFAGFSAESLCERILDSNCSLLVTADAFYrgdklislkQIADDALQKckekgflvrncivVNHLgrEELVSD 242
Cdd:TIGR02262 77 RAGIVPVALNTLLTADDYAYMLEDSRARVVFVSGALL---------PVIKAALGK-------------SPHL--EHRVVV 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 243 GTtsqsppvkrlcqdvqmPWNSDVDLwwHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGyMIFTAATF 322
Cdd:TIGR02262 133 GR----------------PEAGEVQL--AELLATESEQFKPAATQADDPAFWLYSSGSTGMPKGVVHTHSN-PYWTAELY 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 323 -KYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLFEGVPTyPDvsRMWNIVDKYKVTKLYTSPTAIRALMksG 401
Cdd:TIGR02262 194 aRNTLGIREDDVCFSAAKLFFAYGLGNALTFPMSVGATTVLMGERPT-PD--AVFDRLRRHQPTIFYGVPTLYAAML--A 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 402 DEPVKKCSRESLKILGTVGEPINPEAWL-WYYHVIGDercpIVDTFWQTETGGHMLTPLPAvtAMKPGSATFPFFGVVPA 480
Cdd:TIGR02262 269 DPNLPSEDQVRLRLCTSAGEALPAEVGQrWQARFGVD----IVDGIGSTEMLHIFLSNLPG--DVRYGTSGKPVPGYRLR 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 481 ILDESGQELEGEAEGFLVFKQPWPGIMrtVYGNYKRFETTYFKkfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLL 560
Cdd:TIGR02262 343 LVGDGGQDVADGEPGELLISGPSSATM--YWNNRAKSRDTFQG---EWTRSGDKYVRNDDGSYTYAGRTDDMLKVSGIYV 417
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 561 STAEVESALAEHKAISEAAVVSHPHP---VKGEclyCFVTLRDGHEfskTLQEELKKQVREKIGPIATPDYIQHAPGLPK 637
Cdd:TIGR02262 418 SPFEIESALIQHPAVLEAAVVGVADEdglIKPK---AFVVLRPGQT---ALETELKEHVKDRLAPYKYPRWIVFVDDLPK 491
|
570
....*....|...
gi 1394780554 638 TRSGKILRRVLRQ 650
Cdd:TIGR02262 492 TATGKIQRFKLRE 504
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
2-650 |
3.33e-45 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 172.19 E-value: 3.33e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 2 APEEA-----GRL--YRAPAALArEAHVSSLAQYEKLYRRSVEEPHEFWGDIAKEFYWKSH-HSGPFLQYNFDVTKGKif 73
Cdd:PLN03052 90 SPEIAkltnlGRLleARGKELLG-SKYKDPISSFSEFQRFSVENPEVYWSIVLDELSLVFSvPPRCILDTSDESNPGG-- 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 74 vEWMKGSTTNICYNLLdrNVNEKKLGDKIAFYW--EGNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMI 151
Cdd:PLN03052 167 -QWLPGAVLNVAECCL--TPKPSKTDDSIAIIWrdEGSDDLPVNRMTLSELRSQVSRVANALDALGFEKGDAIAIDMPMN 243
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 152 IELVVAMLAcarigalhsIVFAG---------FSAESLCERILDSNCSLLVTADAFYRGDKLISLKQIADDAlqkckeKG 222
Cdd:PLN03052 244 VHAVIIYLA---------IILAGcvvvsiadsFAPSEIATRLKISKAKAIFTQDVIVRGGKSIPLYSRVVEA------KA 308
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 223 FLVrncIVvnhlgreeLVSDGTtsqsppvkrlcqDVQMPWNSDvDLWWHELMRNAS-----KECEPEWCDSEDELFILYT 297
Cdd:PLN03052 309 PKA---IV--------LPADGK------------SVRVKLREG-DMSWDDFLARANglrrpDEYKAVEQPVEAFTNILFS 364
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 298 SGSTGKPKGVVHTVGGYMIFTAATFKYVfDYHPEDIY-WCTgDIGWITGHsYLTYGPLANGATSVLFEGVPTYPDVSRMw 376
Cdd:PLN03052 365 SGTTGEPKAIPWTQLTPLRAAADAWAHL-DIRKGDIVcWPT-NLGWMMGP-WLVYASLLNGATLALYNGSPLGRGFAKF- 440
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 377 niVDKYKVTKLYTSPTAIRALMKSGdePVKKCSRESLKILGTVGEPINPEAWLW-----YYHvigdercPIVDTFWQTET 451
Cdd:PLN03052 441 --VQDAKVTMLGTVPSIVKTWKNTN--CMAGLDWSSIRCFGSTGEASSVDDYLWlmsraGYK-------PIIEYCGGTEL 509
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 452 G-----GHMLTPlPAVTAMKPGSATFPFFgvvpaILDESGQELEGEAEGF-------LVFkqpwpGIMRTVYgNYKRFET 519
Cdd:PLN03052 510 GggfvtGSLLQP-QAFAAFSTPAMGCKLF-----ILDDSGNPYPDDAPCTgelalfpLMF-----GASSTLL-NADHYKV 577
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 520 tYFKKFPGYYVT-----GDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESAL-AEHKAISEAAVVSHPHPVKG-ECL 592
Cdd:PLN03052 578 -YFKGMPVFNGKilrrhGDIFERTSGGYYRAHGRADDTMNLGGIKVSSVEIERVCnAADESVLETAAIGVPPPGGGpEQL 656
|
650 660 670 680 690 700
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1394780554 593 YCFVTLRDGHEFSKTLqEELKK----QVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:PLN03052 657 VIAAVLKDPPGSNPDL-NELKKifnsAIQKKLNPLFKVSAVVIVPSFPRTASNKVMRRVLRQ 717
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
288-652 |
2.46e-44 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 164.66 E-value: 2.46e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 288 SEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFkYVFDYHPEDIYWCTGDIGWiTGHSYLT-YGPLANGATSVLFegv 366
Cdd:cd05974 84 ADDPMLLYFTSGTTSKPKLVEHTHRSYPVGHLSTM-YWIGLKPGDVHWNISSPGW-AKHAWSCfFAPWNAGATVFLF--- 158
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 367 pTYP--DVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSREslkILGTvGEPINPEawlwyyhVIGDER----C 440
Cdd:cd05974 159 -NYArfDAKRVLAALVRYGVTTLCAPPTVWRMLIQQDLASFDVKLRE---VVGA-GEPLNPE-------VIEQVRrawgL 226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 441 PIVDTFWQTETGGhMLTPLPAvTAMKPGSATFPFFGVVPAILDESGQELEgEAEGFLVFKQPWP-GIMRTVYGNYKRfet 519
Cdd:cd05974 227 TIRDGYGQTETTA-LVGNSPG-QPVKAGSMGRPLPGYRVALLDPDGAPAT-EGEVALDLGDTRPvGLMKGYAGDPDK--- 300
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 520 TYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLR 599
Cdd:cd05974 301 TAHAMRGGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLR 380
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|...
gi 1394780554 600 DGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPgLPKTRSGKILRRVLRQIA 652
Cdd:cd05974 381 AGYEPSPETALEIFRFSRERLAPYKRIRRLEFAE-LPKTISGKIRRVELRRRE 432
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
82-648 |
1.01e-43 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 165.13 E-value: 1.01e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 82 TNICYNLldrNVNEKKLGDKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQ-GIKKGDRISIYMPMIIELVVAMLA 160
Cdd:PRK08314 10 TSLFHNL---EVSARRYPDKTAIVFYGR------AISYRELLEEAERLAGYLQQEcGVRKGDRVLLYMQNSPQFVIAYYA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 161 CARIGALHSIVFAGFSAESLCERILDSNCSLLVTADAFYrgDKLISLkqIADDALQkckekgflvrnCIVVNHLGreELV 240
Cdd:PRK08314 81 ILRANAVVVPVNPMNREEELAHYVTDSGARVAIVGSELA--PKVAPA--VGNLRLR-----------HVIVAQYS--DYL 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 241 SDGTTSQSPPVKRLCQDVQMPWNSDVDLWwhELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMiFTAA 320
Cdd:PRK08314 144 PAEPEIAVPAWLRAEPPLQALAPGGVVAW--KEALAAGLAPPPHTAGPDDLAVLPYTSGTTGVPKGCMHTHRTVM-ANAV 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 321 TFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLfegvptypdVSRmWN------IVDKYKVTKLYTSPTAI 394
Cdd:PRK08314 221 GSVLWSNSTPESVVLAVLPLFHVTGMVHSMNAPIYAGATVVL---------MPR-WDreaaarLIERYRVTHWTNIPTMV 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 395 RALMKSGDepVKKCSRESLKILGTVGEPInPEAwlwyyhvIGD---ERC--PIVDTFWQTETGGHMLTPLPAvtAMKPGS 469
Cdd:PRK08314 291 VDFLASPG--LAERDLSSLRYIGGGGAAM-PEA-------VAErlkELTglDYVEGYGLTETMAQTHSNPPD--RPKLQC 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 470 ATFPFFGVVPAILD-ESGQEL-EGEaEGFLVFKQPwpGIMRtvyGNYKRFETT--YFKKFPG--YYVTGDGCRRDKDGYY 543
Cdd:PRK08314 359 LGIPTFGVDARVIDpETLEELpPGE-VGEIVVHGP--QVFK---GYWNRPEATaeAFIEIDGkrFFRTGDLGRMDEEGYF 432
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 544 WITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEfSKTLQEELKKQVREKIGPI 623
Cdd:PRK08314 433 FITDRLKRMINASGFKVWPAEVENLLYKHPAIQEACVIATPDPRRGETVKAVVVLRPEAR-GKTTEEEIIAWAREHMAAY 511
|
570 580
....*....|....*....|....*
gi 1394780554 624 ATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:PRK08314 512 KYPRIVEFVDSLPKSGSGKILWRQL 536
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
96-650 |
8.10e-42 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 159.30 E-value: 8.10e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 96 KKLGDKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGF 175
Cdd:PRK07656 16 RRFGDKEAYVFGDQ------RLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRY 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 176 SAESLCERILDSNCSLLVTADAFyrgdklislkqiaddalqkckekgflvrncivvnhLGREELVSDGTtsqsPPVKRL- 254
Cdd:PRK07656 90 TADEAAYILARGDAKALFVLGLF-----------------------------------LGVDYSATTRL----PALEHVv 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 255 -CQDVQMPWNSDVDLWWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYvFDYHPEDI 333
Cdd:PRK07656 131 iCETEEDDPHTEKMKTFTDFLAAGDPAERAPEVDPDDVADILFTSGTTGRPKGAMLTHRQLLSNAADWAEY-LGLTEGDR 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 334 YWCTG--------DIGWITghsyltygPLANGATSVLfegVPTYpDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPV 405
Cdd:PRK07656 210 YLAANpffhvfgyKAGVNA--------PLMRGATILP---LPVF-DPDEVFRLIETERITVLPGPPTMYNSLLQHPDRSA 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 406 KKCSreSLKILGTVGEPInPEAWLwyyHVIGDE-RCPIVDTfwqtetgGHMLTPLPAVTAM---------KPGSATFPFF 475
Cdd:PRK07656 278 EDLS--SLRLAVTGAASM-PVALL---ERFESElGVDIVLT-------GYGLSEASGVTTFnrldddrktVAGTIGTAIA 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 476 GVVPAILDESGQELEGEAEGFLVFKQPwpGIMRtvyGNYKRFE-TTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLN 554
Cdd:PRK07656 345 GVENKIVNELGEEVPVGEVGELLVRGP--NVMK---GYYDDPEaTAAAIDADGWLHTGDLGRLDEEGYLYIVDRKKDMFI 419
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 555 VSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPG 634
Cdd:PRK07656 420 VGGFNVYPAEVEEVLYEHPAVAEAAVIGVPDERLGEVGKAYVVLKPGAELT---EEELIAYCREHLAKYKVPRSIEFLDE 496
|
570
....*....|....*.
gi 1394780554 635 LPKTRSGKILRRVLRQ 650
Cdd:PRK07656 497 LPKNATGKVLKRALRE 512
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
100-648 |
3.47e-41 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 156.15 E-value: 3.47e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGAlhsiVFAGFSAES 179
Cdd:cd05930 2 DAVAVVDGDQ------SLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGA----AYVPLDPSY 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 180 LCERIL----DSNCSLLVTadafyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlc 255
Cdd:cd05930 72 PAERLAyileDSGAKLVLT------------------------------------------------------------- 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 256 qdvqmpwnsdvdlwwhelmrnaskecepewcDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAAtFKYVFDYHPEDIYW 335
Cdd:cd05930 91 -------------------------------DPDDLAYVIYTSGSTGKPKGVMVEHRGLVNLLLW-MQEAYPLTPGDRVL 138
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 336 CTGDIGWItGHSYLTYGPLANGATSVLfegVP--TYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKcsreSL 413
Cdd:cd05930 139 QFTSFSFD-VSVWEIFGALLAGATLVV---LPeeVRKDPEALADLLAEEGITVLHLTPSLLRLLLQELELAALP----SL 210
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 414 KILGTVGEPINPEAWLWYYHVIGDER---------CPIVDTFWQTETGGHMLTPLPAvtamkpGSatfPFFGVVPAILDE 484
Cdd:cd05930 211 RLVLVGGEALPPDLVRRWRELLPGARlvnlygpteATVDATYYRVPPDDEEDGRVPI------GR---PIPNTRVYVLDE 281
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 485 SGQELEGEAEGFLVFKQPwpGIMRtvyGNYKRFETTYFK-----KFPG--YYVTGDGCRRDKDG--YYwiTGRIDDMLNV 555
Cdd:cd05930 282 NLRPVPPGVPGELYIGGA--GLAR---GYLNRPELTAERfvpnpFGPGerMYRTGDLVRWLPDGnlEF--LGRIDDQVKI 354
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 556 SGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATPDYIQHAPGL 635
Cdd:cd05930 355 RGYRIELGEIEAALLAHPGVREAAVVAREDGDGEKRLVAYVVPDEGGELDE---EELRAHLAERLPDYMVPSAFVVLDAL 431
|
570
....*....|...
gi 1394780554 636 PKTRSGKILRRVL 648
Cdd:cd05930 432 PLTPNGKVDRKAL 444
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
148-653 |
3.08e-39 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 151.51 E-value: 3.08e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 148 MPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTADAFYRGDKLISL-KQIADDALQKCkekgflvr 226
Cdd:PLN03051 1 MPMTVDAVIIYLAIVLAGCVVVSVADSFSAKEIATRLDISGAKGVFTQDVVLRGGRALPLySKVVEAAPAKA-------- 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 227 ncIVVNHLGReelvsdgttsqsppvkrlcqDVQMPWNSDvDLWWHELMRNASK-------ECEPEWCDSEDELFILYTSG 299
Cdd:PLN03051 73 --IVLPAAGE--------------------PVAVPLREQ-DLSWCDFLGVAAAqgsvggnEYSPVYAPVESVTNILFSSG 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 300 STGKPKGVVHTVGGYMIFTAATFKYVfDYHPEDIY-WCTgDIGWITGhSYLTYGPLANGATSVLFEGVPTYPDVSRMwni 378
Cdd:PLN03051 130 TTGEPKAIPWTHLSPLRCASDGWAHM-DIQPGDVVcWPT-NLGWMMG-PWLLYSAFLNGATLALYGGAPLGRGFGKF--- 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 379 VDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEPINPEAWLWYYHVIGDERcPIVDTFWQTETGGHMLTP 458
Cdd:PLN03051 204 VQDAGVTVLGLVPSIVKAWRHTGAFAMEGLDWSKLRVFASTGEASAVDDVLWLSSVRGYYK-PVIEYCGGTELASGYISS 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 459 LPaVTAMKPGSATFPFFGVVPAILDESGQELEGEAE--GFLVFKQPWPGIM-RTVYGNYKRfetTYFKKFPGYYVTGDGC 535
Cdd:PLN03051 283 TL-LQPQAPGAFSTASLGTRFVLLNDNGVPYPDDQPcvGEVALAPPMLGASdRLLNADHDK---VYYKGMPMYGSKGMPL 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 536 RRDKD-------GYYWITGRIDDMLNVSGHLLSTAEVESALAE-HKAISEAAVVSHPHPVKG-ECLYCFVTLRDGHE-FS 605
Cdd:PLN03051 359 RRHGDimkrtpgGYFCVQGRADDTMNLGGIKTSSVEIERACDRaVAGIAETAAVGVAPPDGGpELLVIFLVLGEEKKgFD 438
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 1394780554 606 KTLQEELKKQ----VREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAK 653
Cdd:PLN03051 439 QARPEALQKKfqeaIQTNLNPLFKVSRVKIVPELPRNASNKLLRRVLRDQLK 490
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
114-652 |
1.73e-38 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 151.26 E-value: 1.73e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 114 SMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLAcariGALHSIVFA---GFSAESLCERILDSNCS 190
Cdd:PRK07529 56 PETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWG----GEAAGIANPinpLLEPEQIAELLRAAGAK 131
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 191 LLVTADAFYRGDklisLKQIADDALQKCKEkgflVRNCIVVNHLGREelvsdgttsqsPPVKRLCQDVQMPWNSDVDLWW 270
Cdd:PRK07529 132 VLVTLGPFPGTD----IWQKVAEVLAALPE----LRTVVEVDLARYL-----------PGPKRLAVPLIRRKAHARILDF 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 271 HELMRNASKE-CEPEWCDSEDELFILY-TSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWC-------TGDIG 341
Cdd:PRK07529 193 DAELARQPGDrLFSGRPIGPDDVAAYFhTGGTTGMPKLAQHTHGN-EVANAWLGALLLGLGPGDTVFCglplfhvNALLV 271
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 342 wiTGHSyltygPLANGAtSVLFEGVPTYPD---VSRMWNIVDKYKVTKLYTSPTAIRALMksgDEPVKKCSRESLKILGT 418
Cdd:PRK07529 272 --TGLA-----PLARGA-HVVLATPQGYRGpgvIANFWKIVERYRINFLSGVPTVYAALL---QVPVDGHDISSLRYALC 340
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 419 VGEPINPEAWLWYYHVIGderCPIVDTFWQTE-TGGHMLTPlpAVTAMKPGSA--TFPFFGVVPAILDESG---QELEGE 492
Cdd:PRK07529 341 GAAPLPVEVFRRFEAATG---VRIVEGYGLTEaTCVSSVNP--PDGERRIGSVglRLPYQRVRVVILDDAGrylRDCAVD 415
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 493 AEGFLVFKQPwpgimrTVYGNYKRFETTYFKKF-PGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAE 571
Cdd:PRK07529 416 EVGVLCIAGP------NVFSGYLEAAHNKGLWLeDGWLNTGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPAAIEEALLR 489
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 572 HKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIG-PIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:PRK07529 490 HPAVALAAAVGRPDAHAGELPVAYVQLKPGASAT---EAELLAFARDHIAeRAAVPKHVRILDALPKTAVGKIFKPALRR 566
|
..
gi 1394780554 651 IA 652
Cdd:PRK07529 567 DA 568
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
116-648 |
1.05e-37 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 145.70 E-value: 1.05e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALhsivfagfsaeslcerildsncslLVTA 195
Cdd:cd05935 1 SLTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAV------------------------VVPI 56
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 DAFYRGDKLislKQIADDALQKckekgflvrncIVVNHlgrEELvsdgttsqsppvkrlcqdvqmpwnsdvdlwwhelmr 275
Cdd:cd05935 57 NPMLKEREL---EYILNDSGAK-----------VAVVG---SEL------------------------------------ 83
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 naskecepewcdsEDELFILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLA 355
Cdd:cd05935 84 -------------DDLALIPYTSGTTGLPKGCMHTHFS-AAANALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVY 149
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 356 NGATSVLFegvpTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDepVKKCSRESLKILGTVGEPInPEAWLwyyhvi 435
Cdd:cd05935 150 VGGTYVLM----ARWDRETALELIEKYKVTFWTNIPTMLVDLLATPE--FKTRDLSSLKVLTGGGAPM-PPAVA------ 216
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 436 gdERcpIVDTFWQTETGGHMLTPLPAVTAMKPGSAT------FPFFGVVPAILD-ESGQELEGEAEGFLVFKQPwpGIMR 508
Cdd:cd05935 217 --EK--LLKLTGLRFVEGYGLTETMSQTHTNPPLRPklqclgIP*FGVDARVIDiETGRELPPNEVGEIVVRGP--QIFK 290
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 509 tvyGNYKRFETTY--FKKFPG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHP 584
Cdd:cd05935 291 ---GYWNRPEETEesFIEIKGrrFFRTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVP 367
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1394780554 585 HPVKGECLYCFVTLRDGHEfSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd05935 368 DERVGEEVKAFIVLRPEYR-GKVTEEDIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
116-648 |
1.26e-37 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 147.00 E-value: 1.26e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTA 195
Cdd:cd05904 32 ALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVKDSGAKLAFTT 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 DAFYrgDKLISLKQ---IADDAlqkckekgflvrncivVNHLGREELVSDGTTSQSPPVKRLCQDvqmpwnsdvdlwwhe 272
Cdd:cd05904 112 AELA--EKLASLALpvvLLDSA----------------EFDSLSFSDLLFEADEAEPPVVVIKQD--------------- 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 273 lmrnaskecepewcdseDELFILYTSGSTGKPKGVVHTVGGymiFTAATFKYVFD----YHPEDIYWCTGDIGWITGHSY 348
Cdd:cd05904 159 -----------------DVAALLYSSGTTGRSKGVMLTHRN---LIAMVAQFVAGegsnSDSEDVFLCVLPMFHIYGLSS 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 349 LTYGPLANGATSVLfegVPTYpDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDepVKKCSRESLKILGTVGEPINPEaw 428
Cdd:cd05904 219 FALGLLRLGATVVV---MPRF-DLEELLAAIERYKVTHLPVVPPIVLALVKSPI--VDKYDLSSLRQIMSGAAPLGKE-- 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 429 lwyyhVIGD--ERCPIVDtFWQ----TETGG--HMlTPLPAVTAMKPGSATFPFFGVVPAILD-ESGQELEGEAEGFLVF 499
Cdd:cd05904 291 -----LIEAfrAKFPNVD-LGQgygmTESTGvvAM-CFAPEKDRAKYGSVGRLVPNVEAKIVDpETGESLPPNQTGELWI 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 500 KQPwpGIMRTVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAA 579
Cdd:cd05904 364 RGP--SIMKGYLNNPEATAATIDKE--GWLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAA 439
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1394780554 580 VVSHPHPVKGECLYCFVTLRDGHEFS-KTLQEELKKQVR--EKIGPIATPDYIqhapglPKTRSGKILRRVL 648
Cdd:cd05904 440 VIPYPDEEAGEVPMAFVVRKPGSSLTeDEIMDFVAKQVApyKKVRKVAFVDAI------PKSPSGKILRKEL 505
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
116-650 |
9.34e-36 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 140.21 E-value: 9.34e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHsivfagfsaeslcerildsnCSLLVta 195
Cdd:cd05903 1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVT--------------------NPILP-- 58
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 daFYRGDKLISLkqiaddaLQKCKEKGFLVrncivvnhlgreelvsdgttsqsPPVKRLCQDVQMPwnsdvdlwwhelmr 275
Cdd:cd05903 59 --FFREHELAFI-------LRRAKAKVFVV-----------------------PERFRQFDPAAMP-------------- 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 naskecepewcdsEDELFILYTSGSTGKPKGVVHTVGGYMiftAATFKYVFDYH--PEDIYWCTGDIGWITGHSYLTYGP 353
Cdd:cd05903 93 -------------DAVALLLFTSGTTGEPKGVMHSHNTLS---ASIRQYAERLGlgPGDVFLVASPMAHQTGFVYGFTLP 156
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 354 LANGATSVLfegvptypdvSRMWN------IVDKYKVTKLYTSPT----AIRALMKSGDEPvkkcsrESLKILGTVGEPI 423
Cdd:cd05903 157 LLLGAPVVL----------QDIWDpdkalaLMREHGVTFMMGATPfltdLLNAVEEAGEPL------SRLRTFVCGGATV 220
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 424 NP----EAWlwyyHVIGDERCPIvdtFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVF 499
Cdd:cd05903 221 PRslarRAA----ELLGAKVCSA---YGSTECPGAVTSITPAPEDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELLS 293
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 500 KQPwpgimRTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAA 579
Cdd:cd05903 294 RGP-----SVFLGYLDRPDLTADAAPEGWFRTGDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAA 368
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1394780554 580 VVSHPHPVKGECLYCFVTLRDGHEFS-KTLQEELKKQ--VREKIgpiatPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:cd05903 369 VVALPDERLGERACAVVVTKSGALLTfDELVAYLDRQgvAKQYW-----PERLVHVDDLPRTPSGKVQKFRLRE 437
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
100-648 |
1.39e-35 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 140.14 E-value: 1.39e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAES 179
Cdd:cd17643 2 EAVAVVDEDR------RLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVER 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 180 LCERILDSNCSLLVTadafyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqdvq 259
Cdd:cd17643 76 IAFILADSGPSLLLT----------------------------------------------------------------- 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 260 mpwnsdvdlwwhelmrnaskecepewcDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATfKYVFDYHPEDIywctgd 339
Cdd:cd17643 91 ---------------------------DPDDLAYVIYTSGSTGRPKGVVVSHANVLALFAAT-QRWFGFNEDDV------ 136
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 340 igWITGHSYL-------TYGPLANGATSVLfegVPtyPDVSR----MWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKC 408
Cdd:cd17643 137 --WTLFHSYAfdfsvweIWGALLHGGRLVV---VP--YEVARspedFARLLRDEGVTVLNQTPSAFYQLVEAADRDGRDP 209
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 409 SRESLKILGtvGEPINPeAWL--WYYHViGDERCPIVDTFWQTETGGHMlTPLPAVTAMKPGSATFPFFGVVP----AIL 482
Cdd:cd17643 210 LALRYVIFG--GEALEA-AMLrpWAGRF-GLDRPQLVNMYGITETTVHV-TFRPLDAADLPAAAASPIGRPLPglrvYVL 284
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 483 DESGQELEGEAEGFLVFKQPwpGIMRTVYG----NYKRFETTYFKKfPG--YYVTGDGCRRDKDGYYWITGRIDDMLNVS 556
Cdd:cd17643 285 DADGRPVPPGVVGELYVSGA--GVARGYLGrpelTAERFVANPFGG-PGsrMYRTGDLARRLPDGELEYLGRADEQVKIR 361
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 557 GHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFsktLQEELKKQVREKIgpiatPDYIQHA---- 632
Cdd:cd17643 362 GFRIELGEIEAALATHPSVRDAAVIVREDEPGDTRLVAYVVADDGAAA---DIAELRALLKELL-----PDYMVPAryvp 433
|
570
....*....|....*..
gi 1394780554 633 -PGLPKTRSGKILRRVL 648
Cdd:cd17643 434 lDALPLTVNGKLDRAAL 450
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
100-656 |
8.16e-35 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 139.42 E-value: 8.16e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAES 179
Cdd:PRK13295 39 DKTAVTAVRLGTGAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVLNPLMPIFRERE 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 180 LCERILDSNCSLLVTADAFyRG-DKLISLKQIADDALQkckekgflVRNCIVVNhlgreelvSDGTTSqsppvkrlcqdv 258
Cdd:PRK13295 119 LSFMLKHAESKVLVVPKTF-RGfDHAAMARRLRPELPA--------LRHVVVVG--------GDGADS------------ 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 259 qmpwnsdvdlwWHELMrnaskeCEPEWCDSEDELFIL--------------YTSGSTGKPKGVVHTVGGYMiftAATFKY 324
Cdd:PRK13295 170 -----------FEALL------ITPAWEQEPDAPAILarlrpgpddvtqliYTSGTTGEPKGVMHTANTLM---ANIVPY 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 325 V--FDYHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLFEgvptYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGD 402
Cdd:PRK13295 230 AerLGLGADDVILMASPMAHQTGFMYGLMMPVMLGATAVLQD----IWDPARAAELIRTEGVTFTMASTPFLTDLTRAVK 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 403 EPVKKCSreSLKILGTVGEPINP----EAWlwyyHVIGDErcpIVDTFWQTETGghmltplpAVTAMKPG------SAT- 471
Cdd:PRK13295 306 ESGRPVS--SLRTFLCAGAPIPGalveRAR----AALGAK---IVSAWGMTENG--------AVTLTKLDdpderaSTTd 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 472 -FPFFGVVPAILDESGQELEGEAEGFLVFKQPwpgimrTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRID 550
Cdd:PRK13295 369 gCPLPGVEVRVVDADGAPLPAGQIGRLQVRGC------SNFGGYLKRPQLNGTDADGWFDTGDLARIDADGYIRISGRSK 442
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 551 DMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFS-KTLQEELKKQvreKIGPIATPDYI 629
Cdd:PRK13295 443 DVIIRGGENIPVVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVPRPGQSLDfEEMVEFLKAQ---KVAKQYIPERL 519
|
570 580
....*....|....*....|....*..
gi 1394780554 630 QHAPGLPKTRSGKILRRVLRQIAKNDR 656
Cdd:PRK13295 520 VVRDALPRTPSGKIQKFRLREMLRGED 546
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
96-650 |
4.44e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 137.09 E-value: 4.44e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 96 KKLGDKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALhsIVFAGF 175
Cdd:PRK07470 18 RRFPDRIALVWGDR------SWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAV--WVPTNF 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 176 S---------AESlcerildSNCSLLVTADAFyrgdklislkqiaDDALQKCKEKGFLVRNCIVVnhlgreelvsdGTTS 246
Cdd:PRK07470 90 RqtpdevaylAEA-------SGARAMICHADF-------------PEHAAAVRAASPDLTHVVAI-----------GGAR 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 247 QSPPVKRLcqdvqmpwnsdvdlwwheLMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGgymiftaaTFKYVF 326
Cdd:PRK07470 139 AGLDYEAL------------------VARHLGARVANAAVDHDDPCWFFFTSGTTGRPKAAVLTHG--------QMAFVI 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 327 DYHPEDIYWCTgdigwiTGHS-YLTYGPL------------ANGATSVLfegVPTYP-DVSRMWNIVDKYKVTKLYTSPT 392
Cdd:PRK07470 193 TNHLADLMPGT------TEQDaSLVVAPLshgagihqlcqvARGAATVL---LPSERfDPAEVWALVERHRVTNLFTVPT 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 393 AIRALMKsgDEPVKKCSRESLKILGTVGEPINPEAWLWYYHVIGDErcpIVDTFWQTETGGHMlTPLPA-------VTAM 465
Cdd:PRK07470 264 ILKMLVE--HPAVDRYDHSSLRYVIYAGAPMYRADQKRALAKLGKV---LVQYFGLGEVTGNI-TVLPPalhdaedGPDA 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 466 KPGSATFPFFGVVPAILDESGQELE----GE--AEGFLVFKqpwpGIMRTVYGNYKRFETTYFKkfpgyyvTGDGCRRDK 539
Cdd:PRK07470 338 RIGTCGFERTGMEVQIQDDEGRELPpgetGEicVIGPAVFA----GYYNNPEANAKAFRDGWFR-------TGDLGHLDA 406
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 540 DGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREK 619
Cdd:PRK07470 407 RGFLYITGRASDMYISGGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVD---EAELLAWLDGK 483
|
570 580 590
....*....|....*....|....*....|.
gi 1394780554 620 IGPIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:PRK07470 484 VARYKLPKRFFFWDALPKSGYGKITKKMVRE 514
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
117-648 |
2.33e-32 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 131.83 E-value: 2.33e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTAD 196
Cdd:TIGR03098 26 LTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPINPLLKAEQVAHILADCNVRLLVTSS 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 AfyRGDKLislkqiaDDALQKCkekgflvrncivvnHLGREeLVSDGTTSQSPPvkrlcqdvQMPWNSDVDlwWHELMrN 276
Cdd:TIGR03098 106 E--RLDLL-------HPALPGC--------------HDLRT-LIIVGDPAHASE--------GHPGEEPAS--WPKLL-A 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 ASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGpLAN 356
Cdd:TIGR03098 151 LGDADPPHPVIDSDMAAILYTSGSTGRPKGVVLSHRN-LVAGAQSVATYLENRPDDRLLAVLPLSFDYGFNQLTTA-FYV 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 357 GATSVLFEGVpTYPDVSRMwniVDKYKVTKLYTSPtAIRALMKSGDEPvkKCSRESLKILGTVGEPInPEAWLWYYHvig 436
Cdd:TIGR03098 229 GATVVLHDYL-LPRDVLKA---LEKHGITGLAAVP-PLWAQLAQLDWP--ESAAPSLRYLTNSGGAM-PRATLSRLR--- 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 437 dERCPIVDTFWQ---TETGGHMLTPlPAVTAMKPGS--ATFPFFGVVpaILDESGQELEGEAEGFLVFKQP------WPG 505
Cdd:TIGR03098 298 -SFLPNARLFLMyglTEAFRSTYLP-PEEVDRRPDSigKAIPNAEVL--VLREDGSECAPGEEGELVHRGAlvamgyWND 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 506 IMRTVygnyKRFE-TTYFK---KFPGYYV-TGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAV 580
Cdd:TIGR03098 374 PEKTA----ERFRpLPPFPgelHLPELAVwSGDTVRRDEEGFLYFVGRRDEMIKTSGYRVSPTEVEEVAYATGLVAEAVA 449
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1394780554 581 VSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:TIGR03098 450 FGVPDPTLGQAIVLVVTPPGGEELDR---AALLAECRARLPNYMVPALIHVRQALPRNANGKIDRKAL 514
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
100-649 |
8.05e-32 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 130.11 E-value: 8.05e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGndpgisMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAM----LACARIGALHSIVfagf 175
Cdd:PRK06188 27 DRPALVLGD------TRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIgaaqLAGLRRTALHPLG---- 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 176 SAESLCERILDSNCSLLVTADAFYRgdklislkqiaddalqkckEKGflvrncivvnhlgrEELVSdgttsQSPPVKR-- 253
Cdd:PRK06188 97 SLDDHAYVLEDAGISTLIVDPAPFV-------------------ERA--------------LALLA-----RVPSLKHvl 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 254 -LCqdvqmPWNSDVDLWwHELMRNASKECEPEWCDSeDELFILYTSGSTGKPKGVVHTVGGYMIFTAatfkyvfdyhped 332
Cdd:PRK06188 139 tLG-----PVPDGVDLL-AAAAKFGPAPLVAAALPP-DIAGLAYTGGTTGKPKGVMGTHRSIATMAQ------------- 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 333 iyWCTGDIGWITGHSYLTYGPLANGATS----VLFEGVPTYP----DVSRMWNIVDKYKVTKLYTSPTAIRALMksgDEP 404
Cdd:PRK06188 199 --IQLAEWEWPADPRFLMCTPLSHAGGAfflpTLLRGGTVIVlakfDPAEVLRAIEEQRITATFLVPTMIYALL---DHP 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 405 vkKCSRESLKILGTV---GEPINP----EA-------WLWYYhviGDERCPIVDTFWQTETggHMLTPlPAVTAmkpgSA 470
Cdd:PRK06188 274 --DLRTRDLSSLETVyygASPMSPvrlaEAierfgpiFAQYY---GQTEAPMVITYLRKRD--HDPDD-PKRLT----SC 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 471 TFPFFGVVPAILDESGQELE-GEAEGFLVFKqpwPGIMRtvyGNYKRFETT--YFKKfpGYYVTGDGCRRDKDGYYWITG 547
Cdd:PRK06188 342 GRPTPGLRVALLDEDGREVAqGEVGEICVRG---PLVMD---GYWNRPEETaeAFRD--GWLHTGDVAREDEDGFYYIVD 413
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 548 RIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGhefSKTLQEELKKQVREKIGPIATPD 627
Cdd:PRK06188 414 RKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPG---AAVDAAELQAHVKERKGSVHAPK 490
|
570 580
....*....|....*....|..
gi 1394780554 628 YIQHAPGLPKTRSGKILRRVLR 649
Cdd:PRK06188 491 QVDFVDSLPLTALGKPDKKALR 512
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
100-648 |
8.78e-32 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 128.90 E-value: 8.78e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAfYWEGNDPgismsITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAES 179
Cdd:cd05945 6 DRPA-VVEGGRT-----LTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAER 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 180 LcERILD-SNCSLLVTAdafyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqdv 258
Cdd:cd05945 80 I-REILDaAKPALLIAD--------------------------------------------------------------- 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 259 qmpwnsdvdlwwhelmrnaskecepewcdsEDELF-ILYTSGSTGKPKGVVHTVGGYMIFT---------------AATF 322
Cdd:cd05945 96 ------------------------------GDDNAyIIFTSGSTGRPKGVQISHDNLVSFTnwmlsdfplgpgdvfLNQA 145
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 323 KYVFDYhpediywCTGDIgwitghsyltYGPLANGATSV------------LFEGVPTYPdvsrmwnivdkykVTKLYTS 390
Cdd:cd05945 146 PFSFDL-------SVMDL----------YPALASGATLVpvprdatadpkqLFRFLAEHG-------------ITVWVST 195
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 391 PTAIRALMKSGDEpvkkcSRESLKILGTV---GEPInP----EAWLwyyhvigdER---CPIVDTFWQTET----GGHML 456
Cdd:cd05945 196 PSFAAMCLLSPTF-----TPESLPSLRHFlfcGEVL-PhktaRALQ--------QRfpdARIYNTYGPTEAtvavTYIEV 261
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 457 TPlPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPwpgimrTVYGNY-KRFETT--YFKKFPGY--YVT 531
Cdd:cd05945 262 TP-EVLDGYDRLPIGYAKPGAKLVILDEDGRPVPPGEKGELVISGP------SVSKGYlNNPEKTaaAFFPDEGQraYRT 334
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 532 GDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLqeE 611
Cdd:cd05945 335 GDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIAFVVPKPGAEAGLTK--A 412
|
570 580 590
....*....|....*....|....*....|....*..
gi 1394780554 612 LKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd05945 413 IKAELAERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
118-656 |
9.18e-32 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 129.54 E-value: 9.18e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVtada 197
Cdd:PRK09088 24 TYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEPRLLL---- 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 198 fyrgdklislkqiADDALQKckekgflvrncivvnhlGREELVSdgttsqsppVKRLCQDVQMpwnsdvdlwwHELMRNA 277
Cdd:PRK09088 100 -------------GDDAVAA-----------------GRTDVED---------LAAFIASADA----------LEPADTP 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 278 SkecepewCDSEDELFILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANG 357
Cdd:PRK09088 131 S-------IPPERVSLILFTSGTSGQPKGVMLSERN-LQQTAHNFGVLGRVDAHSSFLCDAPMFHIIGLITSVRPVLAVG 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 358 ATSVLFEGVPtyPDVSRMWNIVDKYKVTKLYTSPTAIRALMksgDEPVKKCSR-ESLKILGTVGEPiNPE----AWLwyy 432
Cdd:PRK09088 203 GSILVSNGFE--PKRTLGRLGDPALGITHYFCVPQMAQAFR---AQPGFDAAAlRHLTALFTGGAP-HAAedilGWL--- 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 433 hvigDERCPIVDTFWQTETGGHMLTPL-PAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQP--WPGIMRT 509
Cdd:PRK09088 274 ----DDGIPMVDGFGMSEAGTVFGMSVdCDVIRAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLLRGPnlSPGYWRR 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 510 VYGNYKRFETTyfkkfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKG 589
Cdd:PRK09088 350 PQATARAFTGD------GWFRTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWG 423
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1394780554 590 ECLYCFVTLRDGhefSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAKNDR 656
Cdd:PRK09088 424 EVGYLAIVPADG---APLDLERIRSHLSTRLAKYKVPKHLRLVDALPRTASGKLQKARLRDALAAGR 487
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
118-650 |
1.25e-31 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 129.67 E-value: 1.25e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGA-LHSIvfagfsaeslcerildsNCSLlvtad 196
Cdd:cd12119 27 TYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAvLHTI-----------------NPRL----- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 afyrgdkliSLKQIAddalqkckekgflvrncIVVNHLGREELVSDGT--------TSQSPPVKR---LCQDVQMPWNSD 265
Cdd:cd12119 85 ---------FPEQIA-----------------YIINHAEDRVVFVDRDflplleaiAPRLPTVEHvvvMTDDAAMPEPAG 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 266 VDLW--WHELMRNASKECEPEWcDSEDELFILYTSGSTGKPKGVV--H--TVGGYMIFTAATFKYVfdyHPEDIY----- 334
Cdd:cd12119 139 VGVLayEELLAAESPEYDWPDF-DENTAAAICYTSGTTGNPKGVVysHrsLVLHAMAALLTDGLGL---SESDVVlpvvp 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 335 ------WCTGDIGWITGHSYLTYGPLANGATsvLFEgvptypdvsrmwnIVDKYKVTKLYTSPTAIRALMKSGDEpvKKC 408
Cdd:cd12119 215 mfhvnaWGLPYAAAMVGAKLVLPGPYLDPAS--LAE-------------LIEREGVTFAAGVPTVWQGLLDHLEA--NGR 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 409 SRESLKILGTVGEPINP---EAWlwyyhvigDERcpIVDTF--W-QTETG--GHMLTPLPAVTAMKPG-------SATFP 473
Cdd:cd12119 278 DLSSLRRVVIGGSAVPRsliEAF--------EER--GVRVIhaWgMTETSplGTVARPPSEHSNLSEDeqlalraKQGRP 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 474 FFGVVPAILDESGQELE--GEAEGFLVFKQPWpgimrtVYGNY-KRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRID 550
Cdd:cd12119 348 VPGVELRIVDDDGRELPwdGKAVGELQVRGPW------VTKSYyKNDEESEALTEDGWLRTGDVATIDEDGYLTITDRSK 421
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 551 DMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATPDYIQ 630
Cdd:cd12119 422 DVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGATVTA---EELLEFLADKVAKWWLPDDVV 498
|
570 580
....*....|....*....|
gi 1394780554 631 HAPGLPKTRSGKILRRVLRQ 650
Cdd:cd12119 499 FVDEIPKTSTGKIDKKALRE 518
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
116-650 |
1.61e-31 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 129.49 E-value: 1.61e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTA 195
Cdd:PRK13382 68 TLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTSFAGPALAEVVTREGVDTVIYD 147
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 DAFyrgdklislKQIADDALQKCkekgflvrncivvnhlgreelvsdgttsqsPPVKRLcqdvqMPWNSDVDLWWHELMR 275
Cdd:PRK13382 148 EEF---------SATVDRALADC------------------------------PQATRI-----VAWTDEDHDLTVEVLI 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 NASKECEPEWCDSEDELfILYTSGSTGKPKGVVHT-VGGYMiftaaTFKYVFDYHPediywctgdigWITGHSYLTYGPL 354
Cdd:PRK13382 184 AAHAGQRPEPTGRKGRV-ILLTSGTTGTPKGARRSgPGGIG-----TLKAILDRTP-----------WRAEEPTVIVAPM 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 355 --ANGATSVLFEGVPTYPDVSR-------MWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEPINP 425
Cdd:PRK13382 247 fhAWGFSQLVLAASLACTIVTRrrfdpeaTLDLIDRHRATGLAVVPVMFDRIMDLPAEVRNRYSGRSLRFAAASGSRMRP 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 426 EAWLWYYHVIGDercPIVDTFWQTETGghML-TPLPAVTAMKPGSATFPFFGVVPAILDESGQEL-EGEAEGFLVFKQpw 503
Cdd:PRK13382 327 DVVIAFMDQFGD---VIYNNYNATEAG--MIaTATPADLRAAPDTAGRPAEGTEIRILDQDFREVpTGEVGTIFVRND-- 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 504 pgimrTVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSH 583
Cdd:PRK13382 400 -----TQFDGYTSGSTKDFHD--GFMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGV 472
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1394780554 584 PHPVKGECLYCFVTLRDGhefSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:PRK13382 473 DDEQYGQRLAAFVVLKPG---ASATPETLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQA 536
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
296-652 |
4.48e-31 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 124.90 E-value: 4.48e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 296 YTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTGDIGWITGhSYLTYG-PLANGAtSVLFEGVPTYPD--- 371
Cdd:cd05944 9 HTGGTTGTPKLAQHTHSN-EVYNAWMLALNSLFDPDDVLLCGLPLFHVNG-SVVTLLtPLASGA-HVVLAGPAGYRNpgl 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 372 VSRMWNIVDKYKVTKLYTSPTAIRALMKSgdePVKKcSRESLKILGTVGEPINPEAWLWYYHVIGderCPIVDTFWQTE- 450
Cdd:cd05944 86 FDNFWKLVERYRITSLSTVPTVYAALLQV---PVNA-DISSLRFAMSGAAPLPVELRARFEDATG---LPVVEGYGLTEa 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 451 TGGHMLTPlPAvTAMKPGSA--TFPFFGVVPAILDESGQELEgEAEGFLVFkqpwPGIM--RTVYGNYKRFETTYFKKF- 525
Cdd:cd05944 159 TCLVAVNP-PD-GPKRPGSVglRLPYARVRIKVLDGVGRLLR-DCAPDEVG----EICVagPGVFGGYLYTEGNKNAFVa 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 526 PGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFS 605
Cdd:cd05944 232 DGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAVVE 311
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 1394780554 606 ktlQEELKKQVREKIGP-IATPDYIQHAPGLPKTRSGKILRRVLRQIA 652
Cdd:cd05944 312 ---EEELLAWARDHVPErAAVPKHIEVLEELPVTAVGKVFKPALRADA 356
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
100-649 |
6.80e-31 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 127.07 E-value: 6.80e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAES 179
Cdd:cd17651 10 DAPALVAEGR------RLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYPAER 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 180 LCERILDSNCSLLVTADAFyrgdklislkqiADDALQkckEKGFLVRncivvnhLGREELVSDGTTSQSPPVkrlcqdvq 259
Cdd:cd17651 84 LAFMLADAGPVLVLTHPAL------------AGELAV---ELVAVTL-------LDQPGAAAGADAEPDPAL-------- 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 260 mpwnsdvdlwwhelmrnaskecepewcDSEDELFILYTSGSTGKPKGVV------------HTVGGYMIFTAATFKYV-- 325
Cdd:cd17651 134 ---------------------------DADDLAYVIYTSGSTGRPKGVVmphrslanlvawQARASSLGPGARTLQFAgl 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 326 -FDYHPEDIYwctgdigwitghSYLTYgplanGATSVLfegvPT---YPDVSRMWNIVDKYKVTKLYTSPTAIRALmksg 401
Cdd:cd17651 187 gFDVSVQEIF------------STLCA-----GATLVL----PPeevRTDPPALAAWLDEQRISRVFLPTVALRAL---- 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 402 DEPVKKCSRES--LKILGTVGEPINPEAWLWYYhvIGDERCP-IVDTFWQTETggHMLTP--LPAVTAMKPGSATF--PF 474
Cdd:cd17651 242 AEHGRPLGVRLaaLRYLLTGGEQLVLTEDLREF--CAGLPGLrLHNHYGPTET--HVVTAlsLPGDPAAWPAPPPIgrPI 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 475 FGVVPAILDESGQELEGEAEGFLVFKQpwPGIMRtvygNY--------KRFETTYFKKFPGYYVTGDGCRRDKDGYYWIT 546
Cdd:cd17651 318 DNTRVYVLDAALRPVPPGVPGELYIGG--AGLAR----GYlnrpeltaERFVPDPFVPGARMYRTGDLARWLPDGELEFL 391
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 547 GRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATP 626
Cdd:cd17651 392 GRADDQVKIRGFRIELGEIEAALARHPGVREAVVLAREDRPGEKRLVAYVVGDPEAPVDA---AELRAALATHLPEYMVP 468
|
570 580
....*....|....*....|...
gi 1394780554 627 DYIQHAPGLPKTRSGKILRRVLR 649
Cdd:cd17651 469 SAFVLLDALPLTPNGKLDRRALP 491
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
116-653 |
7.41e-31 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 127.18 E-value: 7.41e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHsiVFAGFS-----AESLCERildSNCS 190
Cdd:COG1021 50 RLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAIP--VFALPAhrraeISHFAEQ---SEAV 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 191 LLVTADAF----YRGdklislkqIADDALQKCKEkgflVRNCIVVNHLGreELVSdgttsqsppvkrlcqdvqmpwnsdv 266
Cdd:COG1021 125 AYIIPDRHrgfdYRA--------LARELQAEVPS----LRHVLVVGDAG--EFTS------------------------- 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 267 dlwWHELMRNASKECEPEwCDSEDELFILYTSGSTGKPKGVVHTVGGYmIFTAATFKYVFDYHPEDIYWCTGDIGwitgH 346
Cdd:COG1021 166 ---LDALLAAPADLSEPR-PDPDDVAFFQLSGGTTGLPKLIPRTHDDY-LYSVRASAEICGLDADTVYLAALPAA----H 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 347 SY-LTY----GPLANGATSVLFEGvpTYPDVsrMWNIVDKYKVTklYTS--PTAIRALMKSGDEPVKKCSreSLKILGTV 419
Cdd:COG1021 237 NFpLSSpgvlGVLYAGGTVVLAPD--PSPDT--AFPLIERERVT--VTAlvPPLALLWLDAAERSRYDLS--SLRVLQVG 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 420 GEPINPEA----------WLwyYHVIG--------------DERcpivdtfwQTETGGHMLTPLPAVTamkpgsatfpff 475
Cdd:COG1021 309 GAKLSPELarrvrpalgcTL--QQVFGmaeglvnytrlddpEEV--------ILTTQGRPISPDDEVR------------ 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 476 gvvpaILDESGQEL-EGEaEGFLVFKQPWpgimrTVYGnykrfettYFKKfP----------GYYVTGDGCRRDKDGYYW 544
Cdd:COG1021 367 -----IVDEDGNPVpPGE-VGELLTRGPY-----TIRG--------YYRA-PehnaraftpdGFYRTGDLVRRTPDGYLV 426
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 545 ITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGE--CLycFVTLRdGHEFSKtlqEELKKQVREKiGp 622
Cdd:COG1021 427 VEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGErsCA--FVVPR-GEPLTL---AELRRFLRER-G- 498
|
570 580 590
....*....|....*....|....*....|....
gi 1394780554 623 IAT---PDYIQHAPGLPKTRSGKILRRVLRQIAK 653
Cdd:COG1021 499 LAAfklPDRLEFVDALPLTAVGKIDKKALRAALA 532
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
126-649 |
1.79e-30 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 125.25 E-value: 1.79e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 126 VCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAeslcerildsncsLLVTADAFYrgdkli 205
Cdd:cd05922 3 VSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRLGLVFVPLNP-------------TLKESVLRY------ 63
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 206 slkqIADDAlqkckeKGFLVrncivvnhlgreeLVSDGTTSQSPPVKRLCQDVQMPWnsDVDLWWHELMRNASKECEPEw 285
Cdd:cd05922 64 ----LVADA------GGRIV-------------LADAGAADRLRDALPASPDPGTVL--DADGIRAARASAPAHEVSHE- 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 286 cdseDELFILYTSGSTGKPKGVV--HTvggYMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGpLANGATSVLF 363
Cdd:cd05922 118 ----DLALLLYTSGSTGSPKLVRlsHQ---NLLANARSIAEYLGITADDRALTVLPLSYDYGLSVLNTH-LLRGATLVLT 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 364 E-GVPtyPDVsrMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKcsrESLKILGTVGEPINPE--------AWLWYYHV 434
Cdd:cd05922 190 NdGVL--DDA--FWEDLREHGATGLAGVPSTYAMLTRLGFDPAKL---PSLRYLTQAGGRLPQEtiarlrelLPGAQVYV 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 435 IgdercpivdtFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPwpgimrTVYGNY 514
Cdd:cd05922 263 M----------YGQTEATRRMTYLPPERILEKPGSIGLAIPGGEFEILDDDGTPTPPGEPGEIVHRGP------NVMKGY 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 515 KRFETTYFKK--FPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVkGECL 592
Cdd:cd05922 327 WNDPPYRRKEgrGGGVLHTGDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPDPL-GEKL 405
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 1394780554 593 YCFVTLRDGHEFSktlqeELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:cd05922 406 ALFVTAPDKIDPK-----DVLRSLAERLPPYKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
117-643 |
2.64e-30 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 125.92 E-value: 2.64e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQL--LQDvcQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVT 194
Cdd:PRK06178 59 ITYAELdeLSD--RFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHELSYELNDAGAEVLLA 136
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 195 ADAFYrgdklislkQIADDALQKCKekgflVRNCIVVNhlgreelVSDGTTSQspPVKRLCQDVQMPWNSDVDlwWHELM 274
Cdd:PRK06178 137 LDQLA---------PVVEQVRAETS-----LRHVIVTS-------LADVLPAE--PTLPLPDSLRAPRLAAAG--AIDLL 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 275 R--NASKECEPEWCDSEDELFIL-YTSGSTGKPKGVVHTvGGYMIFTAATFKYVFDYHPED--------IYWCTG-DIGW 342
Cdd:PRK06178 192 PalRACTAPVPLPPPALDALAALnYTGGTTGMPKGCEHT-QRDMVYTAAAAYAVAVVGGEDsvflsflpEFWIAGeNFGL 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 343 ITghsyltygPLANGATSVLF---------EGVPTYpDVSRMWNIVDKYkvTKLYTSPTAIRALMKSGDEP-----VKKC 408
Cdd:PRK06178 271 LF--------PLFSGATLVLLarwdavafmAAVERY-RVTRTVMLVDNA--VELMDHPRFAEYDLSSLRQVrvvsfVKKL 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 409 SRESLK----ILGTVgepINPEAWlwyyhviGDERCPIVDTFwqT---ETGGHMLTPLPAvtamkpgsatfpFFGV-VPA 480
Cdd:PRK06178 340 NPDYRQrwraLTGSV---LAEAAW-------GMTETHTCDTF--TagfQDDDFDLLSQPV------------FVGLpVPG 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 481 ----ILD-ESGQELEGEAEGFLVFKQPwpgimrTVY-GNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLN 554
Cdd:PRK06178 396 tefkICDfETGELLPLGAEGEIVVRTP------SLLkGYWNKPEATAEALRDGWLHTGDIGKIDEQGFLHYLGRRKEMLK 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 555 VSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDyIQHAPG 634
Cdd:PRK06178 470 VNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGADLT---AAALQAWCRENMAVYKVPE-IRIVDA 545
|
....*....
gi 1394780554 635 LPKTRSGKI 643
Cdd:PRK06178 546 LPMTATGKV 554
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
118-580 |
2.69e-30 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 123.91 E-value: 2.69e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQ-GIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLcERIL-DSNCSLLVTA 195
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERL-AFILeDAGARLLLTD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 DAFyrgdklislkqiaddalqkCKEKGFLVRNCIVVNHLgREELVSDGTTSQSPPVKrlcqdvqmpwnsdvdlwwhelmr 275
Cdd:TIGR01733 80 SAL-------------------ASRLAGLVLPVILLDPL-ELAALDDAPAPPPPDAP----------------------- 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 naskecepewCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYvFDYHPEDIywctgdigWITGHSYL------ 349
Cdd:TIGR01733 117 ----------SGPDDLAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLARR-YGLDPDDR--------VLQFASLSfdasve 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 350 -TYGPLANGATSVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEpvkkcSRESLKILGTVGEPINPEA- 427
Cdd:TIGR01733 178 eIFGALLAGATLVVPPEDEERDDAALLAALIAEHPVTVLNLTPSLLALLAAALPP-----ALASLRLVILGGEALTPALv 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 428 --WLWYYHvigdeRCPIVDTFWQTETGGH-MLTPLPAVTAMKPGSATF--PFFGVVPAILDESGQELEGEAEGFLVFKQp 502
Cdd:TIGR01733 253 drWRARGP-----GARLINLYGPTETTVWsTATLVDPDDAPRESPVPIgrPLANTRLYVLDDDLRPVPVGVVGELYIGG- 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 503 wPGIMRtvyGNYKRFETT--YFKKFPGY-------YVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHK 573
Cdd:TIGR01733 327 -PGVAR---GYLNRPELTaeRFVPDPFAggdgarlYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHP 402
|
....*..
gi 1394780554 574 AISEAAV 580
Cdd:TIGR01733 403 GVREAVV 409
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
97-649 |
5.88e-30 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 124.41 E-value: 5.88e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 97 KLGDKIAFYWEgNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFS 176
Cdd:PRK08008 19 VYGHKTALIFE-SSGGVVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLL 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 177 AESlCERILDsNC--SLLVTADAFY------RGDKLISLKQIaddalqkckekgflvrnCIVVNHLGREELVSDGTTSQS 248
Cdd:PRK08008 98 REE-SAWILQ-NSqaSLLVTSAQFYpmyrqiQQEDATPLRHI-----------------CLTRVALPADDGVSSFTQLKA 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 249 PPVKRLCQDVqmPWNSDvdlwwhelmrnaskecepewcdseDELFILYTSGSTGKPKGVVHT-----VGGYmiFTAatfk 323
Cdd:PRK08008 159 QQPATLCYAP--PLSTD------------------------DTAEILFTSGTTSRPKGVVIThynlrFAGY--YSA---- 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 324 yvfdyhpediyWCTG----DIgwitghsYLTYGP--------------LANGATSVLFEgvpTYpDVSRMWNIVDKYKVT 385
Cdd:PRK08008 207 -----------WQCAlrddDV-------YLTVMPafhidcqctaamaaFSAGATFVLLE---KY-SARAFWGQVCKYRAT 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 386 KLYTSPTAIRALMKSGDEPVKK--CSRESLkilgtvgepinpeawlWYYHVIGDERcpivDTFWQ------------TET 451
Cdd:PRK08008 265 ITECIPMMIRTLMVQPPSANDRqhCLREVM----------------FYLNLSDQEK----DAFEErfgvrlltsygmTET 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 452 GGHMLTPLPAVTAMKPgSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPwPGimRTVY-GNYKRFETTYFKKFP-GYY 529
Cdd:PRK08008 325 IVGIIGDRPGDKRRWP-SIGRPGFCYEAEIRDDHNRPLPAGEIGEICIKGV-PG--KTIFkEYYLDPKATAKVLEAdGWL 400
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 530 VTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQ 609
Cdd:PRK08008 401 HTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLS---E 477
|
570 580 590 600
....*....|....*....|....*....|....*....|
gi 1394780554 610 EELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:PRK08008 478 EEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNLK 517
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
286-650 |
9.70e-30 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 122.78 E-value: 9.70e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 286 CDSEDELF-----ILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPED-------IYWCTGDIGWITGhsyltygP 353
Cdd:cd05941 81 TDSEPSLVldpalILYTSGTTGRPKGVVLTHAN-LAANVRALVDAWRWTEDDvllhvlpLHHVHGLVNALLC-------P 152
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 354 LANGATSV--------------------LFEGVPT-YPDVSRMWNIVDKYKVTKLYTSPTAIRaLMKSGD----EPVKkc 408
Cdd:cd05941 153 LFAGASVEflpkfdpkevaisrlmpsitVFMGVPTiYTRLLQYYEAHFTDPQFARAAAAERLR-LMVSGSaalpVPTL-- 229
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 409 sRESLKILGtvgepinpeawlwyyHVIgDERcpivdtFWQTETGghMLTPLPAVTAMKPGSATFPFFGVVPAILDES-GQ 487
Cdd:cd05941 230 -EEWEAITG---------------HTL-LER------YGMTEIG--MALSNPLDGERRPGTVGMPLPGVQARIVDEEtGE 284
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 488 ELEGEAEGFLVFKQPwpgimrTVYGNY-KRFETTYfKKFP--GYYVTGDGCRRDKDGYYWITGRI-DDMLNVSGHLLSTA 563
Cdd:cd05941 285 PLPRGEVGEIQVRGP------SVFKEYwNKPEATK-EEFTddGWFKTGDLGVVDEDGYYWILGRSsVDIIKSGGYKVSAL 357
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 564 EVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEfSKTLqEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKI 643
Cdd:cd05941 358 EIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRAGAA-ALSL-EELKEWAKQRLAPYKRPRRLILVDELPRNAMGKV 435
|
....*..
gi 1394780554 644 LRRVLRQ 650
Cdd:cd05941 436 NKKELRK 442
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
100-648 |
2.01e-29 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 123.22 E-value: 2.01e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGNDpgismsITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAES 179
Cdd:PRK06710 39 EKKALHFLGKD------ITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLYTERE 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 180 LCERILDSNCSLLVTAD-AFYRGDKLISLKQIADDALQKCKEKGFLVRNCIV-VNHLGREELVSDGTTSQSPPVkrlcqd 257
Cdd:PRK06710 113 LEYQLHDSGAKVILCLDlVFPRVTNVQSATKIEHVIVTRIADFLPFPKNLLYpFVQKKQSNLVVKVSESETIHL------ 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 258 vqmpWNSdvdlwwHELMRNASKECEpewCDSEDELFIL-YTSGSTGKPKGVVHTVGGYMIFTAATFKYVFD-YHPEDIYW 335
Cdd:PRK06710 187 ----WNS------VEKEVNTGVEVP---CDPENDLALLqYTGGTTGFPKGVMLTHKNLVSNTLMGVQWLYNcKEGEEVVL 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 336 CTGDIGWITGHSYLTYGPLANGATSVLfegVPTYpDVSRMWNIVDKYKVTKLYTSPTAIRALMKSgdEPVKKCSRESLKI 415
Cdd:PRK06710 254 GVLPFFHVYGMTAVMNLSIMQGYKMVL---IPKF-DMKMVFEAIKKHKVTLFPGAPTIYIALLNS--PLLKEYDISSIRA 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 416 LGTVGEPINPEAWLWYYHVIG---------DERCPIVDT--FWQTETGGHMLTPLPAVTAMkpgsatfpffgvvpAILDE 484
Cdd:PRK06710 328 CISGSAPLPVEVQEKFETVTGgklvegyglTESSPVTHSnfLWEKRVPGSIGVPWPDTEAM--------------IMSLE 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 485 SGQELEGEAEGFLVFKQPwpGIMRtvyGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAE 564
Cdd:PRK06710 394 TGEALPPGEIGEIVVKGP--QIMK---GYWNKPEETAAVLQDGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPRE 468
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 565 VESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKIL 644
Cdd:PRK06710 469 VEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVVLKEGTECS---EEELNQFARKYLAAYKVPKVYEFRDELPKTTVGKIL 545
|
....
gi 1394780554 645 RRVL 648
Cdd:PRK06710 546 RRVL 549
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
116-648 |
4.55e-29 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 121.24 E-value: 4.55e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLcERIL-DSNCSLLVT 194
Cdd:cd12116 12 SLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRL-RYILeDAEPALVLT 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 195 adafyrgdklislkqiaDDALQkckekgFLVRNCIVVNHLGREELVSDGTTSQSPPvkrlcqdvqmpwnsdvdlwwhelm 274
Cdd:cd12116 91 -----------------DDALP------DRLPAGLPVLLLALAAAAAAPAAPRTPV------------------------ 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 275 rnaskecepewcDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAAtFKYVFDYHPED----IYWCTGDIgwitghSYLT 350
Cdd:cd12116 124 ------------SPDDLAYVIYTSGSTGRPKGVVVSHRNLVNFLHS-MRERLGLGPGDrllaVTTYAFDI------SLLE 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 351 -YGPLANGATSVLFEGVPTYpDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPvkkcsRESLKIL-GtvGEPINPEaw 428
Cdd:cd12116 185 lLLPLLAGARVVIAPRETQR-DPEALARLIEAHSITVMQATPATWRMLLDAGWQG-----RAGLTALcG--GEALPPD-- 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 429 lwyyhvIGDERCPIVDTFWQ----TETgghmlTPLPAVTAMKPGSATFPFFGVVPA----ILDESGQELegeAEGflVFK 500
Cdd:cd12116 255 ------LAARLLSRVGSLWNlygpTET-----TIWSTAARVTAAAGPIPIGRPLANtqvyVLDAALRPV---PPG--VPG 318
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 501 QPW---PGIMRtvyGNYKRFETTyFKKF-------PG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESA 568
Cdd:cd12116 319 ELYiggDGVAQ---GYLGRPALT-AERFvpdpfagPGsrLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAA 394
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 569 LAEHKAISEAAVVSHPHPVKGEcLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd12116 395 LAAHPGVAQAAVVVREDGGDRR-LVAYVVLKAGAAPD---AAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
118-649 |
5.17e-29 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 121.25 E-value: 5.17e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALhsivfagfsaeslcerildsNCSLLVTADA 197
Cdd:cd12118 31 TWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAV--------------------LNALNTRLDA 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 198 fyrgdKLISLkqiaddALQKCKEKGFLVrncivvnhlgreelvsdgttsqsppvkrlcqdvqmpwnsDVDLWWHELMRNA 277
Cdd:cd12118 91 -----EEIAF------ILRHSEAKVLFV---------------------------------------DREFEYEDLLAEG 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 278 SKECEPEWCDSEDELFIL-YTSGSTGKPKGVVHTVGGYMIFTAATFkYVFDYHPEDIYWCTGDI----GWItghsyLTYG 352
Cdd:cd12118 121 DPDFEWIPPADEWDPIALnYTSGTTGRPKGVVYHHRGAYLNALANI-LEWEMKQHPVYLWTLPMfhcnGWC-----FPWT 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 353 PLANGATSVLFEGVpTYPDVsrmWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSReslKILGTVGEPINPEAWLWYY 432
Cdd:cd12118 195 VAAVGGTNVCLRKV-DAKAI---YDLIEKHKVTHFCGAPTVLNMLANAPPSDARPLPH---RVHVMTAGAPPPAAVLAKM 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 433 HVIGDERC----------PIVDTFWQTETGGhmlTPLPAVTAMKP--GSATFPFFGVvpAILD-ESGQEL--EGEAEGFL 497
Cdd:cd12118 268 EELGFDVThvygltetygPATVCAWKPEWDE---LPTEERARLKArqGVRYVGLEEV--DVLDpETMKPVprDGKTIGEI 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 498 VFKqpwpG--IMRtvyGNYKRFETTYfKKFP-GYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKA 574
Cdd:cd12118 343 VFR----GniVMK---GYLKNPEATA-EAFRgGWFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPA 414
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1394780554 575 ISEAAVVSHPHPVKGECLYCFVTLRDGhefSKTLQEELKKQVREKIGPIATPDYIQHAPgLPKTRSGKILRRVLR 649
Cdd:cd12118 415 VLEAAVVARPDEKWGEVPCAFVELKEG---AKVTEEEIIAFCREHLAGFMVPKTVVFGE-LPKTSTGKIQKFVLR 485
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
115-649 |
1.97e-28 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 119.62 E-value: 1.97e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 115 MSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVT 194
Cdd:PRK08276 10 EVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLIV 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 195 ADAFyrgdkLISLKQIADDAlqkckEKGFLVRncivvnhlgreeLVSDGTTSqsppvkrlcqdvqmPWNSdvdlwWHELM 274
Cdd:PRK08276 90 SAAL-----ADTAAELAAEL-----PAGVPLL------------LVVAGPVP--------------GFRS-----YEEAL 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 275 RNASKEcepewcDSEDELF---ILYTSGSTGKPKGV--------VHTVGGYMIFTAATFkyvFDYHPEDIYWCTGDIgwi 343
Cdd:PRK08276 129 AAQPDT------PIADETAgadMLYSSGTTGRPKGIkrplpgldPDEAPGMMLALLGFG---MYGGPDSVYLSPAPL--- 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 344 tghsY----LTYG--PLANGATSVLFEGVptypDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILG 417
Cdd:PRK08276 197 ----YhtapLRFGmsALALGGTVVVMEKF----DAEEALALIERYRVTHSQLVPTMFVRMLKLPEEVRARYDVSSLRVAI 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 418 TVGEPINPE------AWlWyyhviGdercPIVD-TFWQTEtgGHMLTPLPAVTAM-KPGSATFPFFGVVpAILDESGQEL 489
Cdd:PRK08276 269 HAAAPCPVEvkramiDW-W-----G----PIIHeYYASSE--GGGVTVITSEDWLaHPGSVGKAVLGEV-RILDEDGNEL 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 490 EGEAEGFLVFKQPWPGImrTVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLnVSGHL-LSTAEVESA 568
Cdd:PRK08276 336 PPGEIGTVYFEMDGYPF--EYHNDPEKTAAARNPH--GWVTVGDVGYLDEDGYLYLTDRKSDMI-ISGGVnIYPQEIENL 410
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 569 LAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:PRK08276 411 LVTHPKVADVAVFGVPDEEMGERVKAVVQPADGADAGDALAAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRL 490
|
.
gi 1394780554 649 R 649
Cdd:PRK08276 491 R 491
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
564-642 |
3.21e-28 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 108.02 E-value: 3.21e-28
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1394780554 564 EVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEfskTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGK 642
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVE---LLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
110-648 |
3.87e-28 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 118.77 E-value: 3.87e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 110 DPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSN- 188
Cdd:cd05923 22 DPARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKAAELAELIERGEm 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 189 CSLLVTADAF-YRGDKLISLKQIADDALqkckekgflvrncivvnhLGREELVSDGTTSQSPPvkrlcqdvqmpwnsdvd 267
Cdd:cd05923 102 TAAVIAVDAQvMDAIFQSGVRVLALSDL------------------VGLGEPESAGPLIEDPP----------------- 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 268 lwwhelmrnaskeCEPEwcdseDELFILYTSGSTGKPKGVV---HTVGGYMIFTAATFKYVFDYHpediywcTGDIGWIT 344
Cdd:cd05923 147 -------------REPE-----QPAFVFYTSGTTGLPKGAVipqRAAESRVLFMSTQAGLRHGRH-------NVVLGLMP 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 345 ghSYLTYGPLANGATSVLFEG---VPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSreSLKILGTVGE 421
Cdd:cd05923 202 --LYHVIGFFAVLVAALALDGtyvVVEEFDPADALKLIEQERVTSLFATPTHLDALAAAAEFAGLKLS--SLRHVTFAGA 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 422 PInPEAWLWYYHvigdeRC---PIVDTFWQTETGGHMLTPLPAV-TAMKPGsatfpFFG---VVPaILDESGQELEGEAE 494
Cdd:cd05923 278 TM-PDAVLERVN-----QHlpgEKVNIYGTTEAMNSLYMRDARTgTEMRPG-----FFSevrIVR-IGGSPDEALANGEE 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 495 GFLVFKQP----WPGIMRtvygnykRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALA 570
Cdd:cd05923 346 GELIVAAAadaaFTGYLN-------QPEATAKKLQDGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLS 418
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 571 EHKAISEAAVVSHPHPVKGECLYCFVTLRDGhefskTLQEELKKQV--REKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd05923 419 RHPGVTEVVVIGVADERWGQSVTACVVPREG-----TLSADELDQFcrASELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
116-651 |
4.40e-28 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 119.26 E-value: 4.40e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTA 195
Cdd:PRK07788 74 TLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSGPQLAEVAAREGVKALVYD 153
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 DAFyrgdklislkqiaDDALQKCKEKgfLVRNCIVVNHlgreelvSDGTTSQSPPVKRLcqdvqmpwnsdvdlwwHELMR 275
Cdd:PRK07788 154 DEF-------------TDLLSALPPD--LGRLRAWGGN-------PDDDEPSGSTDETL----------------DDLIA 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 NASKECEPEWcdSEDELFILYTSGSTGKPKGVVH-------TVGGymIFTAATFKYvfdyhpEDIYWCTGDIGWITGHSY 348
Cdd:PRK07788 196 GSSTAPLPKP--PKPGGIVILTSGTTGTPKGAPRpepsplaPLAG--LLSRVPFRA------GETTLLPAPMFHATGWAH 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 349 LTYGpLANGATSVL---FEGVPTYPDVsrmwnivDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEPINP 425
Cdd:PRK07788 266 LTLA-MALGSTVVLrrrFDPEATLEDI-------AKHKATALVVVPVMLSRILDLGPEVLAKYDTSSLKIIFVSGSALSP 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 426 EAWLWYYHVIGdercPIVDTFW-QTETGgHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGflvfkqpwp 504
Cdd:PRK07788 338 ELATRALEAFG----PVLYNLYgSTEVA-FATIATPEDLAEAPGTVGRPPKGVTVKILDENGNEVPRGVVG--------- 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 505 gimRTVYGNYKRFETtYF-----KKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAA 579
Cdd:PRK07788 404 ---RIFVGNGFPFEG-YTdgrdkQIIDGLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAA 479
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1394780554 580 VVSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQI 651
Cdd:PRK07788 480 VIGVDDEEFGQRLRAFVVKAPGAALDE---DAIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREM 548
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
100-655 |
7.51e-28 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 117.76 E-value: 7.51e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGAlhSIVFAG--FSA 177
Cdd:PRK03640 17 DRTAIEFEEK------KVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGA--VAVLLNtrLSR 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 178 ESLCERILDSNCSLLVTADAFyrGDKLISLKQIAddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqd 257
Cdd:PRK03640 89 EELLWQLDDAEVKCLITDDDF--EAKLIPGISVK---------------------------------------------- 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 258 vqmpwnsdvdlwWHELMRNASKECEP-EWCDSEDELFILYTSGSTGKPKGVVHTVGGYMiFTAATFKYVFDYHPEDIYWC 336
Cdd:PRK03640 121 ------------FAELMNGPKEEAEIqEEFDLDEVATIMYTSGTTGKPKGVIQTYGNHW-WSAVGSALNLGLTEDDCWLA 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 337 TGDIGWITGHSYLTYGpLANGATSVLFEGVptypDVSRMWNIVDKYKVTKLYTSPTAI-RALMKSGDEPvkkCSrESLK- 414
Cdd:PRK03640 188 AVPIFHISGLSILMRS-VIYGMRVVLVEKF----DAEKINKLLQTGGVTIISVVSTMLqRLLERLGEGT---YP-SSFRc 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 415 -ILGtvGEPInPEAWLwyyhvigdERC-----PIVDTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDEsGQE 488
Cdd:PRK03640 259 mLLG--GGPA-PKPLL--------EQCkekgiPVYQSYGMTETASQIVTLSPEDALTKLGSAGKPLFPCELKIEKD-GVV 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 489 LEGEAEGFLVFKQPwpGIMRtvyGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESA 568
Cdd:PRK03640 327 VPPFEEGEIVVKGP--NVTK---GYLNREDATRETFQDGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEV 401
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 569 LAEHKAISEAAVVSHPHPVKGECLYCFVTLrdGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:PRK03640 402 LLSHPGVAEAGVVGVPDDKWGQVPVAFVVK--SGEVT---EEELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHEL 476
|
....*..
gi 1394780554 649 RQIAKND 655
Cdd:PRK03640 477 KQLVEEM 483
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
116-650 |
9.05e-28 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 116.29 E-value: 9.05e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGAlhSIVFAGfsaeslcerildsncsllvta 195
Cdd:cd05912 1 SYTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGA--EAVLLN--------------------- 57
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 dafyrgdklislkqiaddalqkckekgflvrncivvNHLGREELVSdgttsqsppvkrlcqdvQMPwNSDVDLwwhelmr 275
Cdd:cd05912 58 ------------------------------------TRLTPNELAF-----------------QLK-DSDVKL------- 76
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 naskecepewcdsEDELFILYTSGSTGKPKGVVHTVGGYMiFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGpLA 355
Cdd:cd05912 77 -------------DDIATIMYTSGTTGKPKGVQQTFGNHW-WSAIGSALNLGLTEDDNWLCALPLFHISGLSILMRS-VI 141
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 356 NGATSVLFEGVptypDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKkcsrESLK--ILGtvGEPInPEAWLwyyh 433
Cdd:cd05912 142 YGMTVYLVDKF----DAEQVLHLINSGKVTIISVVPTMLQRLLEILGEGYP----NNLRciLLG--GGPA-PKPLL---- 206
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 434 vigdERC-----PIVDTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDEsGQELEGEAEgfLVFKQPwpGIMR 508
Cdd:cd05912 207 ----EQCkekgiPVYQSYGMTETCSQIVTLSPEDALNKIGSAGKPLFPVELKIEDD-GQPPYEVGE--ILLKGP--NVTK 277
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 509 TVYGNYKR----FETTYFKkfpgyyvTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHP 584
Cdd:cd05912 278 GYLNRPDAteesFENGWFK-------TGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIP 350
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1394780554 585 HPVKGECLYCFVTLRdghefSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:cd05912 351 DDKWGQVPVAFVVSE-----RPISEEELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
117-642 |
1.87e-27 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 116.91 E-value: 1.87e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTAD 196
Cdd:PRK07798 29 LTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVEDELRYLLDDSDAVALVYER 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 AFyrGDKLISLKqiadDALQKckekgflVRNCIVVNhlgreelvsDGTTSQSPPvkrlcqdvqmpwnSDVDlwWHELMRN 276
Cdd:PRK07798 109 EF--APRVAEVL----PRLPK-------LRTLVVVE---------DGSGNDLLP-------------GAVD--YEDALAA 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 ASKECEPEwCDSEDELFILYTSGSTGKPKGVVHT--------VGGYMIFTAATfkyvfdyhPEDIYWCTGDIGWITGHSY 348
Cdd:PRK07798 152 GSPERDFG-ERSPDDLYLLYTGGTTGMPKGVMWRqedifrvlLGGRDFATGEP--------IEDEEELAKRAAAGPGMRR 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 349 LTYGPLANGAT-----SVLFEG--VPTYPDVS----RMWNIVDKYKVTKL------YTSPTaIRALMKSGDepvKKCSre 411
Cdd:PRK07798 223 FPAPPLMHGAGqwaafAALFSGqtVVLLPDVRfdadEVWRTIEREKVNVItivgdaMARPL-LDALEARGP---YDLS-- 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 412 SLKILGTVGEPINP---EAWL-WYYHVIgdercpIVDTFWQTETGGHMLtplpAVTAMKPGSATFPFFGVVP--AILDES 485
Cdd:PRK07798 297 SLFAIASGGALFSPsvkEALLeLLPNVV------LTDSIGSSETGFGGS----GTVAKGAVHTGGPRFTIGPrtVVLDED 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 486 GQELE--GEAEGFLVFKQPWPgimrtvYGNYKRFETT--YFKKFPG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHL 559
Cdd:PRK07798 367 GNPVEpgSGEIGWIARRGHIP------LGYYKDPEKTaeTFPTIDGvrYAIPGDRARVEADGTITLLGRGSVCINTGGEK 440
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 560 LSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTR 639
Cdd:PRK07798 441 VFPEEVEEALKAHPDVADALVVGVPDERWGQEVVAVVQLREGARPD---LAELRAHCRSSLAGYKVPRAIWFVDEVQRSP 517
|
...
gi 1394780554 640 SGK 642
Cdd:PRK07798 518 AGK 520
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
100-668 |
2.49e-27 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 116.80 E-value: 2.49e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAES 179
Cdd:PRK07786 32 DAPALRFLGN------TTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPE 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 180 LCERILDSNCSLLVTADAfyrgdkLISLKQIADDALQkckekgfLVRNCIVV------NHLGREELVSDGTTSQSPpvkr 253
Cdd:PRK07786 106 IAFLVSDCGAHVVVTEAA------LAPVATAVRDIVP-------LLSTVVVAggssddSVLGYEDLLAEAGPAHAP---- 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 254 lcqdvqmpwnsdVDLwwhelmrnaskecePEwcDSEDelFILYTSGSTGKPKGVV--HTvggYMIFTAATFKYVFDYHPE 331
Cdd:PRK07786 169 ------------VDI--------------PN--DSPA--LIMYTSGTTGRPKGAVltHA---NLTGQAMTCLRTNGADIN 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 332 DiywctgDIGWITGHSYLTYGpLANGATSVLFeGVPT--YP----DVSRMWNIVDKYKVTKLYTSPTAIRALMksgDEPV 405
Cdd:PRK07786 216 S------DVGFVGVPLFHIAG-IGSMLPGLLL-GAPTviYPlgafDPGQLLDVLEAEKVTGIFLVPAQWQAVC---AEQQ 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 406 KKCSRESLKILGTVGEPINPEAWLWYYHVIGDERcpIVDTFWQTEtgghmLTPlpaVTAM--------KPGSATFPFFGV 477
Cdd:PRK07786 285 ARPRDLALRVLSWGAAPASDTLLRQMAATFPEAQ--ILAAFGQTE-----MSP---VTCMllgedairKLGSVGKVIPTV 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 478 VPAILDESGQELEGEAEGFLVFKQPwpGIMRTVYGNYKRFETTYFKkfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSG 557
Cdd:PRK07786 355 AARVVDENMNDVPVGEVGEIVYRAP--TLMSGYWNNPEATAEAFAG---GWFHSGDLVRQDEEGYVWVVDRKKDMIISGG 429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 558 HLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEfSKTLqEELKKQVREKIGPIATPDYIQHAPGLPK 637
Cdd:PRK07786 430 ENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRNDDA-ALTL-EDLAEFLTDRLARYKHPKALEIVDALPR 507
|
570 580 590
....*....|....*....|....*....|...
gi 1394780554 638 TRSGKILRRVLRQI--AKNDRDLGDTSTVADQS 668
Cdd:PRK07786 508 NPAGKVLKTELRERygACVNVERRSASAGFTER 540
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
116-648 |
7.83e-27 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 113.95 E-value: 7.83e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTa 195
Cdd:cd12115 24 SLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPAYPPERLRFILEDAQARLVLT- 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 dafyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqdvqmpwnsdvdlwwhelmr 275
Cdd:cd12115 --------------------------------------------------------------------------------
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 naskecepewcDSEDELFILYTSGSTGKPKGVV--HTVGGYMIFTAATfkyvfDYHPEDIYwctgdiGWITGHS------ 347
Cdd:cd12115 103 -----------DPDDLAYVIYTSGSTGRPKGVAieHRNAAAFLQWAAA-----AFSAEELA------GVLASTSicfdls 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 348 -YLTYGPLANGATSVLFEGVPTYPDVSRmwnivdKYKVTKLYTSPTAIRALMKSGDEPvkkcsrESLKILGTVGEPINPE 426
Cdd:cd12115 161 vFELFGPLATGGKVVLADNVLALPDLPA------AAEVTLINTVPSAAAELLRHDALP------ASVRVVNLAGEPLPRD 228
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 427 AWLWYYHVIGDERcpIVDTFWQTETgghmlTPLPAVTAMKPGSAT-----FPFFGVVPAILDESGQEL------------ 489
Cdd:cd12115 229 LVQRLYARLQVER--VVNLYGPSED-----TTYSTVAPVPPGASGevsigRPLANTQAYVLDRALQPVplgvpgelyigg 301
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 490 EGEAEGFLvfKQPwpgiMRTVygnyKRFETTYFkkFPG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVES 567
Cdd:cd12115 302 AGVARGYL--GRP----GLTA----ERFLPDPF--GPGarLYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEA 369
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 568 ALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGhefSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRV 647
Cdd:cd12115 370 ALRSIPGVREAVVVAIGDAAGERRLVAYIVAEPG---AAGLVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSA 446
|
.
gi 1394780554 648 L 648
Cdd:cd12115 447 L 447
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
116-581 |
8.14e-27 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 114.23 E-value: 8.14e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESlCERIL-DSNCSLLVT 194
Cdd:cd05907 5 PITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQ-IAYILnDSEAKALFV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 195 ADAfyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqdvqmpwnsdvdlwwhelm 274
Cdd:cd05907 84 EDP----------------------------------------------------------------------------- 86
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 275 rnaskecepewcdseDELF-ILYTSGSTGKPKGVVHTVGGYMiftaATFKYVFDYHP---EDIYWCTGDIGWITGHSYLT 350
Cdd:cd05907 87 ---------------DDLAtIIYTSGTTGRPKGVMLSHRNIL----SNALALAERLPateGDRHLSFLPLAHVFERRAGL 147
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 351 YGPLANGATSVLFEGV-----------PTY-PDVSRMWNIVdkYKVTKLYTSPTAIRALMksgdepvKKCSRESLKILGT 418
Cdd:cd05907 148 YVPLLAGARIYFASSAetllddlsevrPTVfLAVPRVWEKV--YAAIKVKAVPGLKRKLF-------DLAVGGRLRFAAS 218
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 419 VGEPINPEAwLWYYHVIGderCPIVDTFWQTETGG-HMLTPLPAVTAMKPGSatfPFFGVVPAILDesgqelEGEaegfL 497
Cdd:cd05907 219 GGAPLPAEL-LHFFRALG---IPVYEGYGLTETSAvVTLNPPGDNRIGTVGK---PLPGVEVRIAD------DGE----I 281
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 498 VFKQpwPGIMRtvyGNYKRFETTYFKKFP-GYYVTGDGCRRDKDGYYWITGRIDDML-NVSGHLLSTAEVESALAEHKAI 575
Cdd:cd05907 282 LVRG--PNVML---GYYKNPEATAEALDAdGWLHTGDLGEIDEDGFLHITGRKKDLIiTSGGKNISPEPIENALKASPLI 356
|
....*.
gi 1394780554 576 SEAAVV 581
Cdd:cd05907 357 SQAVVI 362
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
118-652 |
8.32e-27 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 115.26 E-value: 8.32e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTADA 197
Cdd:PRK12583 47 TWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRASELEYALGQSGVRWVICADA 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 198 FYRGDKLISLKQIAddalqkckekgflvrncivvnhlgrEELVSDGTTSQSPP-VKRLCQDVQM-PWNSDVDLWWHELM- 274
Cdd:PRK12583 127 FKTSDYHAMLQELL-------------------------PGLAEGQPGALACErLPELRGVVSLaPAPPPGFLAWHELQa 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 275 ------RNASKECEPEwCDSEDELFILYTSGSTGKPKGVVHT----------VGGYMIFTAATFKYVfdyhPEDIYWCTG 338
Cdd:PRK12583 182 rgetvsREALAERQAS-LDRDDPINIQYTSGTTGFPKGATLShhnilnngyfVAESLGLTEHDRLCV----PVPLYHCFG 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 339 DIgwitghsYLTYGPLANGATsVLFEGVPTYPDVSrmWNIVDKYKVTKLYTSPTAIRALMksgDEP-VKKCSRESLKILG 417
Cdd:PRK12583 257 MV-------LANLGCMTVGAC-LVYPNEAFDPLAT--LQAVEEERCTALYGVPTMFIAEL---DHPqRGNFDLSSLRTGI 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 418 TVGEPINPEAwlwYYHVIGDERCP-IVDTFWQTETgghmlTPLPAVTA--------MKPGSATFPFFGVvpAILDESGQE 488
Cdd:PRK12583 324 MAGAPCPIEV---MRRVMDEMHMAeVQIAYGMTET-----SPVSLQTTaaddlerrVETVGRTQPHLEV--KVVDPDGAT 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 489 LEGEAEGFLVFKqpwpG--IMRTVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVE 566
Cdd:PRK12583 394 VPRGEIGELCTR----GysVMKGYWNNPEATAESIDED--GWMHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIE 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 567 SALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRR 646
Cdd:PRK12583 468 EFLFTHPAVADVQVFGVPDEKYGEEIVAWVRLHPGHAAS---EEELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQKF 544
|
....*.
gi 1394780554 647 VLRQIA 652
Cdd:PRK12583 545 RMREIS 550
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
116-654 |
1.05e-26 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 114.57 E-value: 1.05e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQ-EQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVT 194
Cdd:PRK06839 27 EMTYKQLHEYVSKVAAYLIyELNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENELIFQLKDSGTTVLFV 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 195 ADAFYRGDKLISlkqiaddalqkckekgflvrncivvnhlgreelvsdGTTSQSPPVkrlcqdvqmpWNSDVDlwwhELM 274
Cdd:PRK06839 107 EKTFQNMALSMQ------------------------------------KVSYVQRVI----------SITSLK----EIE 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 275 RNASKECEPEwcDSEDELFILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPL 354
Cdd:PRK06839 137 DRKIDNFVEK--NESASFIICYTSGTTGKPKGAVLTQEN-MFWNALNNTFAIDLTMHDRSIVLLPLFHIGGIGLFAFPTL 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 355 ANGATSVlfegVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDepVKKCSRESLKILGTVGEPInPEAWLWYYHv 434
Cdd:PRK06839 214 FAGGVII----VPRKFEPTKALSMIEKHKVTVVMGVPTIHQALINCSK--FETTNLQSVRWFYNGGAPC-PEELMREFI- 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 435 igDERCPIVDTFWQTETGGHMLTPLPAVTAMKPGSATFP-FFGVVPAILDESGQELEGEAEGFLVfkqPWPGIMRTVYGN 513
Cdd:PRK06839 286 --DRGFLFGQGFGMTETSPTVFMLSEEDARRKVGSIGKPvLFCDYELIDENKNKVEVGEVGELLI---RGPNVMKEYWNR 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 514 YKRFETTYFKkfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLY 593
Cdd:PRK06839 361 PDATEETIQD---GWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPI 437
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1394780554 594 CFVTLRDGhefSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAKN 654
Cdd:PRK06839 438 AFIVKKSS---SVLIEKDVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQLVNQLKS 495
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
117-652 |
1.43e-26 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 114.46 E-value: 1.43e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTAD 196
Cdd:PRK06164 36 LSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRYRSHEVAHILGRGRARWLVVWP 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 AFYRGDKLISLKQIADDALQKckekgflVRNCIVVnhlgreelvsDGTTSQSPPvkrlcqdvqmPWNSDvdlwWHELMRN 276
Cdd:PRK06164 116 GFKGIDFAAILAAVPPDALPP-------LRAIAVV----------DDAADATPA----------PAPGA----RVQLFAL 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 ASKECEP---EWCDSEDELFILY-TSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTyG 352
Cdd:PRK06164 165 PDPAPPAaagERAADPDAGALLFtTSGTTSGPKLVLHRQAT-LLRHARAIARAYGYDPGAVLLAALPFCGVFGFSTLL-G 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 353 PLANGATSVLfegVPTYpDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEP-----VKKC--------SRESLKILGTV 419
Cdd:PRK06164 243 ALAGGAPLVC---EPVF-DAARTARALRRHRVTHTFGNDEMLRRILDTAGERadfpsARLFgfasfapaLGELAALARAR 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 420 GEPInpeawlwyYHVIGDER------CPIVDTFWQT--ETGGHMLTPLPAVTAMKPGSAtfpffGVVPAilDESGqELEG 491
Cdd:PRK06164 319 GVPL--------TGLYGSSEvqalvaLQPATDPVSVriEGGGRPASPEARVRARDPQDG-----ALLPD--GESG-EIEI 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 492 EAegflvfkqpwPGIMRTVYGNYkrfETTYFKKFP-GYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALA 570
Cdd:PRK06164 383 RA----------PSLMRGYLDNP---DATARALTDdGYFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALE 449
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 571 EHKAISEAAVVSHPHPVKGEClYCFVTLRDGhefSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSG---KILRRV 647
Cdd:PRK06164 450 ALPGVAAAQVVGATRDGKTVP-VAFVIPTDG---ASPDEAGLMAACREALAGFKVPARVQVVEAFPVTESAngaKIQKHR 525
|
....*
gi 1394780554 648 LRQIA 652
Cdd:PRK06164 526 LREMA 530
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
88-650 |
1.08e-25 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 112.02 E-value: 1.08e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 88 LLDRNVneKKLGDKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGA- 166
Cdd:PRK05605 37 LYDNAV--ARFGDRPALDFFGA------TTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAv 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 167 --LHSIVFAGFSAESLCErilDSNCSLLVTADafyrgdklislkQIADDALQkckekgfLVRNcivvnhLGREELVSDGT 244
Cdd:PRK05605 109 vvEHNPLYTAHELEHPFE---DHGARVAIVWD------------KVAPTVER-------LRRT------TPLETIVSVNM 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 245 TSQSPPVKRLCQDVQMP------------------WNSDVDlwwHELMRNASKECEPEwCDSEDELFILYTSGSTGKPKG 306
Cdd:PRK05605 161 IAAMPLLQRLALRLPIPalrkaraaltgpapgtvpWETLVD---AAIGGDGSDVSHPR-PTPDDVALILYTSGTTGKPKG 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 307 VVHTVGGYMIfTAATFKyvfdyhpediywctgdiGWITG---------------HSY-----LTYGPLAnGATSVLFegv 366
Cdd:PRK05605 237 AQLTHRNLFA-NAAQGK-----------------AWVPGlgdgpervlaalpmfHAYgltlcLTLAVSI-GGELVLL--- 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 367 PTyPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEpvKKCSRESLK--ILGTVGEPInpeawlwyyhvigdercPIVD 444
Cdd:PRK05605 295 PA-PDIDLILDAMKKHPPTWLPGVPPLYEKIAEAAEE--RGVDLSGVRnaFSGAMALPV-----------------STVE 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 445 TfWQTETGGHM-----LT---PL----PAVTAMKPGSATFPF----FGVV-PAILDES---GQELEGEAEGFLVFKqpwp 504
Cdd:PRK05605 355 L-WEKLTGGLLvegygLTetsPIivgnPMSDDRRPGYVGVPFpdteVRIVdPEDPDETmpdGEEGELLVRGPQVFK---- 429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 505 gimrtvyGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHP 584
Cdd:PRK05605 430 -------GYWNRPEETAKSFLDGWFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLP 502
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1394780554 585 HPVKGECLYCFVTLRDGhefsKTLQEE-LKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:PRK05605 503 REDGSEEVVAAVVLEPG----AALDPEgLRAYCREHLTRYKVPRRFYHVDELPRDQLGKVRRREVRE 565
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
88-581 |
1.92e-25 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 111.35 E-value: 1.92e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 88 LLDRNVneKKLGDKIAFYWEGNdpGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGAL 167
Cdd:COG1022 16 LLRRRA--ARFPDRVALREKED--GIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAV 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 168 HSIVFAGFSAESLcERIL-DSNCSLLVTADAFYRgDKLISLKqiadDALQKCKEkgflvrncIVVnhlgreelVSDGTTS 246
Cdd:COG1022 92 TVPIYPTSSAEEV-AYILnDSGAKVLFVEDQEQL-DKLLEVR----DELPSLRH--------IVV--------LDPRGLR 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 247 QSPPVkrlcqdvqmpwnsdvdLWWHELMRNASKECEPEW-------CDSEDELFILYTSGSTGKPKGVVHTVGGyMIFTA 319
Cdd:COG1022 150 DDPRL----------------LSLDELLALGREVADPAElearraaVKPDDLATIIYTSGTTGRPKGVMLTHRN-LLSNA 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 320 ATFKYVFDYHPEDIY-----WCtgdigWITGHSyLTYGPLANGATSVLFEGVPTYPD------------VSRMWNivdky 382
Cdd:COG1022 213 RALLERLPLGPGDRTlsflpLA-----HVFERT-VSYYALAAGATVAFAESPDTLAEdlrevkptfmlaVPRVWE----- 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 383 kvtKLYtspTAIRALMKSGDEPVKKCSRESLKI------------------------------------LG-------TV 419
Cdd:COG1022 282 ---KVY---AGIQAKAEEAGGLKRKLFRWALAVgrryararlagkspslllrlkhaladklvfsklreaLGgrlrfavSG 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 420 GEPINPEAwLWYYHVIGderCPIVDTFWQTETGghmltplPAVT-----AMKPGSATFPFFGVVPAILDesgqelEGEae 494
Cdd:COG1022 356 GAALGPEL-ARFFRALG---IPVLEGYGLTETS-------PVITvnrpgDNRIGTVGPPLPGVEVKIAE------DGE-- 416
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 495 gfLVFKQpwPGIMRtvyGNYKRFETT--------YFKkfpgyyvTGD-GcRRDKDGYYWITGRIDDMLNVS-GHLLSTAE 564
Cdd:COG1022 417 --ILVRG--PNVMK---GYYKNPEATaeafdadgWLH-------TGDiG-ELDEDGFLRITGRKKDLIVTSgGKNVAPQP 481
|
570
....*....|....*..
gi 1394780554 565 VESALAEHKAISEAAVV 581
Cdd:COG1022 482 IENALKASPLIEQAVVV 498
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
117-654 |
2.41e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 110.25 E-value: 2.41e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGiKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTaD 196
Cdd:PRK07638 27 LTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDELKERLAISNADMIVT-E 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 AFYRGDklISlkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqDVQMP-WNSDVdlwWHELMR 275
Cdd:PRK07638 105 RYKLND--LP--------------------------------------------------DEEGRvIEIDE---WKRMIE 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 NASKECEPEwCDSEDELFIL-YTSGSTGKPKGVVHTVGGYMiftaatfkYVFDYHPEDIYWCTGDIGWITG---HSYLTY 351
Cdd:PRK07638 130 KYLPTYAPI-ENVQNAPFYMgFTSGSTGKPKAFLRAQQSWL--------HSFDCNVHDFHMKREDSVLIAGtlvHSLFLY 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 352 GplangATSVLFEGVPTYpdVSRMWNIVD------KYKVTKLYTSPTAIRALMKSG---DEPVK------KCSRESLKIL 416
Cdd:PRK07638 201 G-----AISTLYVGQTVH--LMRKFIPNQvldkleTENISVMYTVPTMLESLYKENrviENKMKiissgaKWEAEAKEKI 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 417 GTvgepINPEAWLWyyhvigdercpivdTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGF 496
Cdd:PRK07638 274 KN----IFPYAKLY--------------EFYGASELSFVTALVDEESERRPNSVGRPFHNVQVRICNEAGEEVQKGEIGT 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 497 LVFKQPwpgiMRtvYGNYKRFETTYFKKFPGYYVT-GDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAI 575
Cdd:PRK07638 336 VYVKSP----QF--FMGYIIGGVLARELNADGWMTvRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAV 409
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1394780554 576 SEAAVVSHPHPVKGECLycfVTLRDGHEFSKTlqeeLKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAKN 654
Cdd:PRK07638 410 DEIVVIGVPDSYWGEKP---VAIIKGSATKQQ----LKSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSWIEN 481
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
116-649 |
2.55e-25 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 112.26 E-value: 2.55e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGAlhsivfA------GFSAESLcERIL-DSN 188
Cdd:COG1020 501 SLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGA------AyvpldpAYPAERL-AYMLeDAG 573
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 189 CSLLVTADAFyrgdklisLKQIADDALQkckekgflvrnCIVVNHLGREElvsdgtTSQSPPVKRLcqdvqmpwnsdvdl 268
Cdd:COG1020 574 ARLVLTQSAL--------AARLPELGVP-----------VLALDALALAA------EPATNPPVPV-------------- 614
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 269 wwhelmrnaskecepewcDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYvFDYHPEDIywctgdigwITGHSY 348
Cdd:COG1020 615 ------------------TPDDLAYVIYTSGSTGRPKGVMVEHRALVNLLAWMQRR-YGLGPGDR---------VLQFAS 666
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 349 LT--------YGPLANGATSVLF-EGVPtyPDVSRMWNIVDKYKVTKLYTSPTAIRALMksgDEPVKKCSreSLKILGTV 419
Cdd:COG1020 667 LSfdasvweiFGALLSGATLVLApPEAR--RDPAALAELLARHRVTVLNLTPSLLRALL---DAAPEALP--SLRLVLVG 739
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 420 GEPINPEAWLWYYHVIGDerCPIVDTFWQTETG----GHMLTPLPAVTAMKP-GSatfPFFGVVPAILDESGQ------- 487
Cdd:COG1020 740 GEALPPELVRRWRARLPG--ARLVNLYGPTETTvdstYYEVTPPDADGGSVPiGR---PIANTRVYVLDAHLQpvpvgvp 814
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 488 -EL----EGEAEGFLvfKQPwpgiMRTVygnyKRFETTYFkKFPG--YYVTGDGCRRDKDG---YywiTGRIDDMLNVSG 557
Cdd:COG1020 815 gELyiggAGLARGYL--NRP----ELTA----ERFVADPF-GFPGarLYRTGDLARWLPDGnleF---LGRADDQVKIRG 880
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 558 HLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEfskTLQEELKKQVREKIGPIATPDYIQHAPGLPK 637
Cdd:COG1020 881 FRIELGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAA---AAAALLRLALALLLPPYMVPAAVVLLLPLPL 957
|
570
....*....|..
gi 1394780554 638 TRSGKILRRVLR 649
Cdd:COG1020 958 TGNGKLDRLALP 969
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
293-649 |
2.69e-25 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 109.38 E-value: 2.69e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 293 FILYTSGSTGKPKGVVHTVGGYMIFTAATFKYvFDYHPEDIYWCTGDIGWITGHSYLtYGPLANGAtSVLFEGVPTYPDV 372
Cdd:cd17649 98 YVIYTSGSTGTPKGVAVSHGPLAAHCQATAER-YGLTPGDRELQFASFNFDGAHEQL-LPPLICGA-CVVLRPDELWASA 174
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 373 SRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKcSRESLKILGTVGEPINPE-AWLWyyhviGDERCPIVDTFWQTET 451
Cdd:cd17649 175 DELAEMVRELGVTVLDLPPAYLQQLAEEADRTGDG-RPPSLRLYIFGGEALSPElLRRW-----LKAPVRLFNAYGPTEA 248
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 452 gghMLTPL--PAVTAMKPGSATFPFFGVVPA----ILDESGQELEGEAEGFLVFKQPwpGIMRtvyGNYKRFETTYfKKF 525
Cdd:cd17649 249 ---TVTPLvwKCEAGAARAGASMPIGRPLGGrsayILDADLNPVPVGVTGELYIGGE--GLAR---GYLGRPELTA-ERF 319
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 526 -------PG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVkGECLYCFV 596
Cdd:cd17649 320 vpdpfgaPGsrLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDGAG-GKQLVAYV 398
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|...
gi 1394780554 597 TLRDGHEFSKTLqEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:cd17649 399 VLRAAAAQPELR-AQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
116-650 |
6.17e-25 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 109.45 E-value: 6.17e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALhsivfagfsaeslcerildsncslLVTA 195
Cdd:PRK06087 49 SYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAV------------------------SVPL 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 DAFYRGDKLISlkqiaddALQKCKEKGFLvrnCIVVNHLGREELVSDGTTSQSPPVKRLCQ-DVQMPWNSDVDLWwHELM 274
Cdd:PRK06087 105 LPSWREAELVW-------VLNKCQAKMFF---APTLFKQTRPVDLILPLQNQLPQLQQIVGvDKLAPATSSLSLS-QIIA 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 275 RNASKECEPewCDSEDELF-ILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGP 353
Cdd:PRK06087 174 DYEPLTTAI--TTHGDELAaVLFTSGTEGLPKGVMLTHNN-ILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAP 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 354 LANGATSVLFEGVPtyPDVSrmWNIVDKYKVTKLYTSPTAIRALMKSGDEpvKKCSRESLKILGTVGEPInPEAWL---W 430
Cdd:PRK06087 251 FLIGARSVLLDIFT--PDAC--LALLEQQRCTCMLGATPFIYDLLNLLEK--QPADLSALRFFLCGGTTI-PKKVArecQ 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 431 YYHVIgdercpIVDTFWQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQEL----EGE--AEGFLVFkqpwp 504
Cdd:PRK06087 324 QRGIK------LLSVYGSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDEARKTLppgcEGEeaSRGPNVF----- 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 505 gimrTVYGNYKRfETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHP 584
Cdd:PRK06087 393 ----MGYLDEPE-LTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMP 467
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1394780554 585 HPVKGECLYCFVTLRDgHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:PRK06087 468 DERLGERSCAYVVLKA-PHHSLTLEEVVAFFSRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRK 532
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
96-648 |
7.36e-25 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 108.44 E-value: 7.36e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 96 KKLGDKIAFYWEGndpgisMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGF 175
Cdd:cd12117 8 ARTPDAVAVVYGD------RSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPEL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 176 SAESLCERILDSNCSLLVTadafyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsDGTTSQSPPVKRLC 255
Cdd:cd12117 82 PAERLAFMLADAGAKVLLT-----------------------------------------------DRSLAGRAGGLEVA 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 256 QDVQMPWnsdvdlwwhelmRNASKECEPEWCDSEDELFILYTSGSTGKPKGV-------VHTV--GGYMIFTaatfkyvf 326
Cdd:cd12117 115 VVIDEAL------------DAGPAGNPAVPVSPDDLAYVMYTSGSTGRPKGVavthrgvVRLVknTNYVTLG-------- 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 327 dyhPEDIYWCTGDIGWiTGHSYLTYGPLANGATSVLFE-GVPTYPDVSRmwNIVDKYKVTKLYTSPTAIRALMKSGDEpv 405
Cdd:cd12117 175 ---PDDRVLQTSPLAF-DASTFEIWGALLNGARLVLAPkGTLLDPDALG--ALIAEEGVTVLWLTAALFNQLADEDPE-- 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 406 kkcSRESLKILGTVGEPINPE---AWLwyyhvigdERCP---IVDTFWQTETGG----HMLTPLPAVTAMKP-GSatfPF 474
Cdd:cd12117 247 ---CFAGLRELLTGGEVVSPPhvrRVL--------AACPglrLVNGYGPTENTTfttsHVVTELDEVAGSIPiGR---PI 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 475 FGVVPAILDESGQ--------EL----EGEAEGFLvfKQPwpgimrtvYGNYKRFETTYFkkFPG--YYVTGDGCRRDKD 540
Cdd:cd12117 313 ANTRVYVLDEDGRpvppgvpgELyvggDGLALGYL--NRP--------ALTAERFVADPF--GPGerLYRTGDLARWLPD 380
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 541 GYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEfsktlQEELKKQVREKI 620
Cdd:cd12117 381 GRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVVREDAGGDKRLVAYVVAEGALD-----AAELRAFLRERL 455
|
570 580
....*....|....*....|....*...
gi 1394780554 621 GPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd12117 456 PAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
287-652 |
1.10e-24 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 108.19 E-value: 1.10e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKyVFDYHPEDIywctgdigwITG-----HSY-LTYG---PLANG 357
Cdd:cd05909 145 QPDDPAVILFTSGSEGLPKGVVLSHKNLLANVEQITA-IFDPNPEDV---------VFGalpffHSFgLTGClwlPLLSG 214
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 358 AtSVLFEGVPTYPDvsRMWNIVDKYKVTKLYTSPTAIRALMKSgdepVKKCSRESLKILGTVGEPINP---EAWLWYYHV 434
Cdd:cd05909 215 I-KVVFHPNPLDYK--KIPELIYDKKATILLGTPTFLRGYARA----AHPEDFSSLRLVVAGAEKLKDtlrQEFQEKFGI 287
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 435 igdercPIVDTFWQTEtgghmLTPLPAVT----AMKPGSATFPFFGVVPAILD-ESGQELEGEAEGFLVFKQpwPGIMRt 509
Cdd:cd05909 288 ------RILEGYGTTE-----CSPVISVNtpqsPNKEGTVGRPLPGMEVKIVSvETHEEVPIGEGGLLLVRG--PNVML- 353
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 510 vyGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAI-SEAAVVSHPHPVK 588
Cdd:cd05909 354 --GYLNEPELTSFAFGDGWYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEILPEdNEVAVVSVPDGRK 431
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1394780554 589 GECLYCFVTLRDghefskTLQEELKKQVRE-KIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIA 652
Cdd:cd05909 432 GEKIVLLTTTTD------TDPSSLNDILKNaGISNLAKPSYIHQVEEIPLLGTGKPDYVTLKALA 490
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
116-649 |
1.40e-24 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 107.85 E-value: 1.40e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTA 195
Cdd:PRK13391 24 VVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARALITS 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 DAFYrgdklislkQIADDALQKCKEkgflVRNCIVVNHLGReelvSDGTTSQSPPVKRLcqdvqmpwnsdvdlwwhelmr 275
Cdd:PRK13391 104 AAKL---------DVARALLKQCPG----VRHRLVLDGDGE----LEGFVGYAEAVAGL--------------------- 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 naskecePEW-CDSEDE-LFILYTSGSTGKPKGVVHTVGGYMIFTA----ATFKYVFDYHPEDIYWCTGDIGwitgHSyl 349
Cdd:PRK13391 146 -------PATpIADESLgTDMLYSSGTTGRPKGIKRPLPEQPPDTPlpltAFLQRLWGFRSDMVYLSPAPLY----HS-- 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 350 tyGPLA-------NGATSVLFEGVptypDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEP 422
Cdd:PRK13391 213 --APQRavmlvirLGGTVIVMEHF----DAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEEVRDKYDLSSLEVAIHAAAP 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 423 INPE------AWlWyyhvigderCPIVDTFW-QTETGGhmltplpaVTAM-------KPGSATFPFFGVvPAILDESGQE 488
Cdd:PRK13391 287 CPPQvkeqmiDW-W---------GPIIHEYYaATEGLG--------FTACdseewlaHPGTVGRAMFGD-LHILDDDGAE 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 489 LegeaegflvfkqPwPGIMRTVYgnYKR---FEttYFK----------KFPGYYVTGDGCRRDKDGYYWITGRIDDMLNV 555
Cdd:PRK13391 348 L------------P-PGEPGTIW--FEGgrpFE--YLNdpaktaearhPDGTWSTVGDIGYVDEDGYLYLTDRAAFMIIS 410
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 556 SGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGL 635
Cdd:PRK13391 411 GGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDGVDPGPALAAELIAFCRQRLSRQKCPRSIDFEDEL 490
|
570
....*....|....
gi 1394780554 636 PKTRSGKILRRVLR 649
Cdd:PRK13391 491 PRLPTGKLYKRLLR 504
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
117-655 |
1.50e-24 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 108.11 E-value: 1.50e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGA-LH---------SIVFAGFSAESlceRIL- 185
Cdd:PRK08162 44 RTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAvLNtlntrldaaSIAFMLRHGEA---KVLi 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 186 -DSNCSLLVT-ADAFYRGDKLISLkQIADDALQKCKEKGflvrncivvnHLGREELVSDGttsqsppvkrlcqDVQMPWN 263
Cdd:PRK08162 121 vDTEFAEVAReALALLPGPKPLVI-DVDDPEYPGGRFIG----------ALDYEAFLASG-------------DPDFAWT 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 264 SDVDlwwhelmrnaskecepEWcdseDELFILYTSGSTGKPKGVV-HTVGGYMIFTAATFKYVFDYHPedIY-W------ 335
Cdd:PRK08162 177 LPAD----------------EW----DAIALNYTSGTTGNPKGVVyHHRGAYLNALSNILAWGMPKHP--VYlWtlpmfh 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 336 CTGdigW-----ITghsyltygplANGATSVLFEGVptypDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPvkkcsR 410
Cdd:PRK08162 235 CNG---WcfpwtVA----------ARAGTNVCLRKV----DPKLIFDLIREHGVTHYCGAPIVLSALINAPAEW-----R 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 411 ESL--KILGTVGEPINPEAWLWYYHVIGDErcpIVDTFWQTETGGHMltplpAVTAMKPGSATFPFF---------GV-- 477
Cdd:PRK08162 293 AGIdhPVHAMVAGAAPPAAVIAKMEEIGFD---LTHVYGLTETYGPA-----TVCAWQPEWDALPLDeraqlkarqGVry 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 478 ----VPAILD-ESGQEL--EGEAEGFLVFKQpwpgimRTVYGNYKRFETTYFKKFP-GYYVTGDGCRRDKDGYYWITGRI 549
Cdd:PRK08162 365 plqeGVTVLDpDTMQPVpaDGETIGEIMFRG------NIVMKGYLKNPKATEEAFAgGWFHTGDLAVLHPDGYIKIKDRS 438
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 550 DDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDYI 629
Cdd:PRK08162 439 KDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASAT---EEEIIAHCREHLAGFKVPKAV 515
|
570 580
....*....|....*....|....*.
gi 1394780554 630 QHAPgLPKTRSGKILRRVLRQIAKND 655
Cdd:PRK08162 516 VFGE-LPKTSTGKIQKFVLREQAKSL 540
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
294-649 |
2.27e-24 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 107.08 E-value: 2.27e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 294 ILYTSGSTGKPKGVVHTVGGYMIFTAATFKyvfdyhpediywCTGDIGWITGHSYLTYGPL-------------ANGATS 360
Cdd:cd05929 130 MLYSGGTTGRPKGIKRGLPGGPPDNDTLMA------------AALGFGPGADSVYLSPAPLyhaapfrwsmtalFMGGTL 197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 361 VLFEGVptypDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEPINP---EAWL-WYYHVI- 435
Cdd:cd05929 198 VLMEKF----DPEEFLRLIERYRVTFAQFVPTMFVRLLKLPEAVRNAYDLSSLKRVIHAAAPCPPwvkEQWIdWGGPIIw 273
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 436 ----GDER---CPIVDTFWQTetggHmltplpavtamkPGSATFPFFGVVpAILDESGQELEGEAEGFLVFKQPWPgimR 508
Cdd:cd05929 274 eyygGTEGqglTIINGEEWLT----H------------PGSVGRAVLGKV-HILDEDGNEVPPGEIGEVYFANGPG---F 333
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 509 TVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVK 588
Cdd:cd05929 334 EYTNDPEKTAAARNEG--GWSTLGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEEL 411
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1394780554 589 GECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:cd05929 412 GQRVHAVVQPAPGADAGTALAEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLR 472
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
110-656 |
1.38e-23 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 105.31 E-value: 1.38e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 110 DPGISMSITYRQLLQDVCQFASVL-QEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSN 188
Cdd:PLN02574 60 DSSTGFSISYSELQPLVKSMAAGLyHVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCS 139
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 189 CSLLVTADAfyRGDKLISLKqiaddalqkckekgflvrncivVNHLGREELVS-DGTTSQSPPvkrlcqdvqmpwnsdvd 267
Cdd:PLN02574 140 VGLAFTSPE--NVEKLSPLG----------------------VPVIGVPENYDfDSKRIEFPK----------------- 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 268 lwWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHT-------VGGYMIFTAATFKYVfdyHPEDIYWCTGDI 340
Cdd:PLN02574 179 --FYELIKEDFDFVPKPVIKQDDVAAIMYSSGTTGASKGVVLThrnliamVELFVRFEASQYEYP---GSDNVYLAALPM 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 341 GWITGHSYLTYGPLANGATSVLFEGVptypDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGdEPVKKCSRESLKILGTVG 420
Cdd:PLN02574 254 FHIYGLSLFVVGLLSLGSTIVVMRRF----DASDMVKVIDRFKVTHFPVVPPILMALTKKA-KGVCGEVLKSLKQVSCGA 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 421 EPINPEawlwyyhVIGD--ERCPIVDtFWQtetgGHMLTPLPAVTAMKPGSATFPFFGVV--------PAILD-ESGQEL 489
Cdd:PLN02574 329 APLSGK-------FIQDfvQTLPHVD-FIQ----GYGMTESTAVGTRGFNTEKLSKYSSVgllapnmqAKVVDwSTGCLL 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 490 EGEAEGFLvfkqpW---PGIMRTVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVE 566
Cdd:PLN02574 397 PPGNCGEL-----WiqgPGVMKGYLNNPKATQSTIDKD--GWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLE 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 567 SALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRR 646
Cdd:PLN02574 470 AVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGSTLS---QEAVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRR 546
|
570
....*....|
gi 1394780554 647 VLRQIAKNDR 656
Cdd:PLN02574 547 ELKRSLTNSV 556
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
118-648 |
1.61e-23 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 104.33 E-value: 1.61e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALhsIVFAGFS---AE--SLCERildSNCSLL 192
Cdd:cd05920 42 TYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAV--PVLALPShrrSElsAFCAH---AEAVAY 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 193 VTADAFYRGDKlislkqiaddalqkckekgflvrncivvNHLGREELVSdgttsqsppvkrlCQDVQmpwnsdvdlwwhe 272
Cdd:cd05920 117 IVPDRHAGFDH----------------------------RALARELAES-------------IPEVA------------- 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 273 lmrnaskecepewcdsedelFILYTSGSTGKPKGV--VHTVGGYMIFTAATfkyVFDYHPEDIYWCtgdiGWITGHSYLT 350
Cdd:cd05920 143 --------------------LFLLSGGTTGTPKLIprTHNDYAYNVRASAE---VCGLDQDTVYLA----VLPAAHNFPL 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 351 YGP-----LANGATSVLfegvPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSreSLKILGTVGEPINP 425
Cdd:cd05920 196 ACPgvlgtLLAGGRVVL----APDPSPDAAFPLIEREGVTVTALVPALVSLWLDAAASRRADLS--SLRLLQVGGARLSP 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 426 EAWLWYYHVIGderCPIVDTFWQTEtGGHMLTPL---PAVTAMKPGSATFPFFGVVpaILDESGQEL-EGEaEGFLVFKQ 501
Cdd:cd05920 270 ALARRVPPVLG---CTLQQVFGMAE-GLLNYTRLddpDEVIIHTQGRPMSPDDEIR--VVDEEGNPVpPGE-EGELLTRG 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 502 PWpgimrTVYGNYKRFE--TTYFKKfPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAA 579
Cdd:cd05920 343 PY-----TIRGYYRAPEhnARAFTP-DGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAA 416
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1394780554 580 VVSHPHPVKGECLYCFVTLRDghefSKTLQEELKKQVREKigPIAT---PDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd05920 417 VVAMPDELLGERSCAFVVLRD----PPPSAAQLRRFLRER--GLAAyklPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
293-652 |
3.13e-23 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 101.25 E-value: 3.13e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 293 FILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLFEGVPTYPDV 372
Cdd:cd17630 4 TVILTSGSTGTPKAVVHTAAN-LLASAAGLHSRLGFGGGDSWLLSLPLYHVGGLAILVRSLLAGAELVLLERNQALAEDL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 373 SRmwnivdkYKVTklYTS--PTAIRALMKSGDEPvkkcsrESLKILGTV---GEPINPEAwlwyYHVIGDERCPIVDTFW 447
Cdd:cd17630 83 AP-------PGVT--HVSlvPTQLQRLLDSGQGP------AALKSLRAVllgGAPIPPEL----LERAADRGIPLYTTYG 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 448 QTETGGHmltplpaVTAMKPGSATFPFFGVVPaildeSGQELEGEAEGFLvfkqpWPGIMRTVYGNYKRFETTYFKKfPG 527
Cdd:cd17630 144 MTETASQ-------VATKRPDGFGRGGVGVLL-----PGRELRIVEDGEI-----WVGGASLAMGYLRGQLVPEFNE-DG 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 528 YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEfskt 607
Cdd:cd17630 206 WFTTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGPAD---- 281
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 1394780554 608 lQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIA 652
Cdd:cd17630 282 -PAELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
270-649 |
4.40e-23 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 103.57 E-value: 4.40e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 270 WHELMRNASkECEP-EWCDSEDELFILYTSGSTGKPKGVVhTVGGYMIFTAATFKYVFDYHPEDIYWCTGDI-------- 340
Cdd:PRK13388 131 YAELVAAAG-ALTPhREVDAMDPFMLIFTSGTTGAPKAVR-CSHGRLAFAGRALTERFGLTRDDVCYVSMPLfhsnavma 208
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 341 GWitghsyltyGP-LANGATSVL---FEGVPTYPDVSRmwnivdkYKVTKLYTSPTAIRALMKSGDEPVKkcSRESLKI- 415
Cdd:PRK13388 209 GW---------APaVASGAAVALpakFSASGFLDDVRR-------YGATYFNYVGKPLAYILATPERPDD--ADNPLRVa 270
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 416 LGTVGEPINPEAWLWYYhvigdeRCPIVDTFWQTETGGhMLTPLPAVtamKPGSATFPFFGVV-----------PAILDE 484
Cdd:PRK13388 271 FGNEASPRDIAEFSRRF------GCQVEDGYGSSEGAV-IVVREPGT---PPGSIGRGAPGVAiynpetltecaVARFDA 340
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 485 SGQELEG-EAEGFLVFKQPwPGIMRtvyGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTA 563
Cdd:PRK13388 341 HGALLNAdEAIGELVNTAG-AGFFE---GYYNNPEATAERMRHGMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAA 416
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 564 EVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFS-KTLQEELKKQvrEKIGPIATPDYIQHAPGLPKTRSGK 642
Cdd:PRK13388 417 PIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGATFDpDAFAAFLAAQ--PDLGTKAWPRYVRIAADLPSTATNK 494
|
....*..
gi 1394780554 643 ILRRVLR 649
Cdd:PRK13388 495 VLKRELI 501
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
96-657 |
7.14e-23 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 103.21 E-value: 7.14e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 96 KKLGDKIAFywegndpgISMS--ITYRQLLQDVCQFASVLQ-EQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVF 172
Cdd:PRK08974 34 ARYADQPAF--------INMGevMTFRKLEERSRAFAAYLQnGLGLKKGDRVALMMPNLLQYPIALFGILRAGMIVVNVN 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 173 AGFSAESLCERILDSNCSLLVTADAFYRgdkliSLKQIADDAL-----------QKCKEKGFLVRncIVVNHlgreelvs 241
Cdd:PRK08974 106 PLYTPRELEHQLNDSGAKAIVIVSNFAH-----TLEKVVFKTPvkhviltrmgdQLSTAKGTLVN--FVVKY-------- 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 242 dgttsqsppVKRLCQDVQMPwnsdvdlwwHEL-MRNASKE------CEPEwCDSEDELFILYTSGSTGKPKGVVHT---- 310
Cdd:PRK08974 171 ---------IKRLVPKYHLP---------DAIsFRSALHKgrrmqyVKPE-LVPEDLAFLQYTGGTTGVAKGAMLThrnm 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 311 ----------------VGGYMIFTAATFKYVFDyhpediywctgdigwitghsyLTYGPL---ANGATSVLFegvpTYP- 370
Cdd:PRK08974 232 lanleqakaaygpllhPGKELVVTALPLYHIFA---------------------LTVNCLlfiELGGQNLLI----TNPr 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 371 DVSRMWNIVDKYKVTKLYTSPTAIRALMKSgdEPVKKCSRESLKILGTVGEPINPeawlwyyhvigdercpIVDTFWQTE 450
Cdd:PRK08974 287 DIPGFVKELKKYPFTAITGVNTLFNALLNN--EEFQELDFSSLKLSVGGGMAVQQ----------------AVAERWVKL 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 451 TGGHML--------TPLPAVTAMK----PGSATFPffgvVPA----ILDESGQELEGEAEGFLVFKQPwpGIMRtvyGNY 514
Cdd:PRK08974 349 TGQYLLegygltecSPLVSVNPYDldyySGSIGLP----VPSteikLVDDDGNEVPPGEPGELWVKGP--QVML---GYW 419
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 515 KRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYC 594
Cdd:PRK08974 420 QRPEATDEVIKDGWLATGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKI 499
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1394780554 595 FVTLRDghefsKTL-QEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAKNDRD 657
Cdd:PRK08974 500 FVVKKD-----PSLtEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARAKVD 558
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
263-649 |
7.65e-23 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 102.84 E-value: 7.65e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 263 NSDVDLWWHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTvGGYMIFTAATFKYVFDYHPEDIYWCTGDI-- 340
Cdd:PRK07867 126 NVDSPAWADELAAHRDAEPPFRVADPDDLFMLIFTSGTSGDPKAVRCT-HRKVASAGVMLAQRFGLGPDDVCYVSMPLfh 204
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 341 ------GWITGhsyltygpLANGATSVL---FEGVPTYPDVSRM----WNIVDKYKVTKLYTSPTAIRAlmksgDEPvkk 407
Cdd:PRK07867 205 snavmaGWAVA--------LAAGASIALrrkFSASGFLPDVRRYgatyANYVGKPLSYVLATPERPDDA-----DNP--- 268
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 408 csresLKIL-GTVGEPINPEAWlwyyhvigDER--CPIVDTFWQTEtGGHMLTPLPAVtamKPGSATFPFFGVV------ 478
Cdd:PRK07867 269 -----LRIVyGNEGAPGDIARF--------ARRfgCVVVDGFGSTE-GGVAITRTPDT---PPGALGPLPPGVAivdpdt 331
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 479 -----PAILDESGQELEGEAEGFLVFKQPwPGIMRtvyGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDML 553
Cdd:PRK07867 332 gtecpPAEDADGRLLNADEAIGELVNTAG-PGGFE---GYYNDPEADAERMRGGVYWSGDLAYRDADGYAYFAGRLGDWM 407
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 554 NVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEF-SKTLQEELKKQvrEKIGPIATPDYIQHA 632
Cdd:PRK07867 408 RVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPGAKFdPDAFAEFLAAQ--PDLGPKQWPSYVRVC 485
|
410
....*....|....*..
gi 1394780554 633 PGLPKTRSGKILRRVLR 649
Cdd:PRK07867 486 AELPRTATFKVLKRQLS 502
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
117-648 |
2.16e-22 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 103.50 E-value: 2.16e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTAd 196
Cdd:PRK12316 537 LDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYMLEDSGVQLLLSQ- 615
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 afyrgdklislkqiaddalqkckekgflvrncivvNHLGREELVSDGTTsqsppvkRLCQDVQMPWnsdVDLWWHElmrn 276
Cdd:PRK12316 616 -----------------------------------SHLGRKLPLAAGVQ-------VLDLDRPAAW---LEGYSEE---- 646
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 askecEPEWC-DSEDELFILYTSGSTGKPKGVVHT---VGGYMIFTAATFK------------YVFDYHPEDIYWctgdi 340
Cdd:PRK12316 647 -----NPGTElNPENLAYVIYTSGSTGKPKGAGNRhraLSNRLCWMQQAYGlgvgdtvlqktpFSFDVSVWEFFW----- 716
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 341 gwitghsyltygPLANGATSVLF-EGVPTYPDvsRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPvkkcSRESLKILGTV 419
Cdd:PRK12316 717 ------------PLMSGARLVVAaPGDHRDPA--KLVELINREGVDTLHFVPSMLQAFLQDEDVA----SCTSLRRIVCS 778
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 420 GEPINPEAWLWYYHVIGDERcpIVDTFWQTETGghmlTPLPAVTAMKPGSATF----PFFGVVPAILDESGQELEGEAEG 495
Cdd:PRK12316 779 GEALPADAQEQVFAKLPQAG--LYNLYGPTEAA----IDVTHWTCVEEGGDSVpigrPIANLACYILDANLEPVPVGVLG 852
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 496 FLVFKQPwpGIMRTVYG----NYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAE 571
Cdd:PRK12316 853 ELYLAGR--GLARGYHGrpglTAERFVPSPFVAGERMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLE 930
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1394780554 572 HKAISEAAVVShphpVKGECLYCFVTLRDGhefSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:PRK12316 931 HPWVREAAVLA----VDGKQLVGYVVLESE---GGDWREALKAHLAASLPEYMVPAQWLALERLPLTPNGKLDRKAL 1000
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
118-651 |
2.17e-22 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 101.37 E-value: 2.17e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLcERILDSNCSLLVTADA 197
Cdd:PRK06155 48 TYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALRGPQL-EHILRNSGARLLVVEA 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 198 fyrgdKLISLKQIADDALqkckekgflvrncivvnhLGREEL-VSDGTTSQSPPvkRLCQDVQMPwnsdvdlwwhelmrn 276
Cdd:PRK06155 127 -----ALLAALEAADPGD------------------LPLPAVwLLDAPASVSVP--AGWSTAPLP--------------- 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 ASKECEPEWCDSE-DELFILYTSGSTGKPKGVVhtvggymiFTAATFkyvfdyhpediYW----CTGDIGWITGHSYLTY 351
Cdd:PRK06155 167 PLDAPAPAAAVQPgDTAAILYTSGTTGPSKGVC--------CPHAQF-----------YWwgrnSAEDLEIGADDVLYTT 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 352 GPL-------------ANGATSVLFEGVptypDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSResLKILGT 418
Cdd:PRK06155 228 LPLfhtnalnaffqalLAGATYVLEPRF----SASGFWPAVRRHGATVTYLLGAMVSILLSQPARESDRAHR--VRVALG 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 419 VGEPINpeawlwyYHVIGDERC--PIVDTFWQTETGGHMLTPLPAvtaMKPGSATFPFFGVVPAILDESGQELEGEAEGF 496
Cdd:PRK06155 302 PGVPAA-------LHAAFRERFgvDLLDGYGSTETNFVIAVTHGS---QRPGSMGRLAPGFEARVVDEHDQELPDGEPGE 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 497 LVFKQPWPGIMRTVYGNYKRFETTYFKKFpgYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAIS 576
Cdd:PRK06155 372 LLLRADEPFAFATGYFGMPEKTVEAWRNL--WFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVA 449
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1394780554 577 EAAVVSHPHPVKGECLYCFVTLRDGhefsKTLQ-EELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQI 651
Cdd:PRK06155 450 AAAVFPVPSELGEDEVMAAVVLRDG----TALEpVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQ 521
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
88-649 |
5.71e-22 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 100.48 E-value: 5.71e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 88 LLDRNVneKKLGDKIAFywegndpgISM--SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIG 165
Cdd:PRK07059 28 LLEESF--RQYADRPAF--------ICMgkAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAG 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 166 ALHSIVFAGFSAESLCERILDSNCSLLVTADAFYRgdkliSLKQIaddaLQKCKEKGFLVRNC--------IVVNHLGRE 237
Cdd:PRK07059 98 YVVVNVNPLYTPRELEHQLKDSGAEAIVVLENFAT-----TVQQV----LAKTAVKHVVVASMgdllgfkgHIVNFVVRR 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 238 elvsdgttsqsppVKRLCQDVQMP----WNSdvdlwwhELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHT--- 310
Cdd:PRK07059 169 -------------VKKMVPAWSLPghvrFND-------ALAEGARQTFKPVKLGPDDVAFLQYTGGTTGVSKGATLLhrn 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 311 VGGYMIFTAATFKYVFDY--HPEDIYWCTG----DIGWITGHSYLTygpLANGATSVLfegVPTYPDVSRMWNIVDKYKV 384
Cdd:PRK07059 229 IVANVLQMEAWLQPAFEKkpRPDQLNFVCAlplyHIFALTVCGLLG---MRTGGRNIL---IPNPRDIPGFIKELKKYQV 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 385 TKLYTSPTAIRALMKSGDepVKKCSRESLKI-LG---TVGEPInPEAWlwyYHVIGderCPIVDTFWQTETGGhMLTPLP 460
Cdd:PRK07059 303 HIFPAVNTLYNALLNNPD--FDKLDFSKLIVaNGggmAVQRPV-AERW---LEMTG---CPITEGYGLSETSP-VATCNP 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 461 AVTAMKPGSATFPFFGVVPAILDESGQELE-GEAeGFLVFKQPwpgimRTVYGNYKRFETTYFKKFP-GYYVTGDGCRRD 538
Cdd:PRK07059 373 VDATEFSGTIGLPLPSTEVSIRDDDGNDLPlGEP-GEICIRGP-----QVMAGYWNRPDETAKVMTAdGFFRTGDVGVMD 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 539 KDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDghefSKTLQEELKKQVRE 618
Cdd:PRK07059 447 ERGYTKIVDRKKDMILVSGFNVYPNEIEEVVASHPGVLEVAAVGVPDEHSGEAVKLFVVKKD----PALTEEDVKAFCKE 522
|
570 580 590
....*....|....*....|....*....|.
gi 1394780554 619 KIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:PRK07059 523 RLTNYKRPKFVEFRTELPKTNVGKILRRELR 553
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
96-651 |
3.29e-21 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 97.60 E-value: 3.29e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 96 KKLGDKIAFywegNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGalhsiVFAGF 175
Cdd:cd17642 28 ASVPGTIAF----TDAHTGVNYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIG-----VGVAP 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 176 SAESLCERILDSNCSLlvtadafyRGDKLISLKQIADDALQKCKEKGFLVRNCIVVNhlGREELVSdgttsqsppvkRLC 255
Cdd:cd17642 99 TNDIYNERELDHSLNI--------SKPTIVFCSKKGLQKVLNVQKKLKIIKTIIILD--SKEDYKG-----------YQC 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 256 QDVQMPWNSDVDLwwhelmrNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPEDIYW 335
Cdd:cd17642 158 LYTFITQNLPPGF-------NEYDFKPPSFDRDEQVALIMNSSGSTGLPKGVQLTHKNIVARFSHARDPIFGNQIIPDTA 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 336 CTGDIGWITGHSYLT-YGPLANGATSVLfegVPTYPDVSRMWNIVDkYKVTKLYTSPTAIRALMKSgdEPVKKCSRESLK 414
Cdd:cd17642 231 ILTVIPFHHGFGMFTtLGYLICGFRVVL---MYKFEEELFLRSLQD-YKVQSALLVPTLFAFFAKS--TLVDKYDLSNLH 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 415 ILGTVGEPINPEawlwyyhvIGDE-----RCPIV-DTFWQTETGGHML-TPlpaVTAMKPGSA--TFPFFGVVPAILDeS 485
Cdd:cd17642 305 EIASGGAPLSKE--------VGEAvakrfKLPGIrQGYGLTETTSAILiTP---EGDDKPGAVgkVVPFFYAKVVDLD-T 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 486 GQELEGEAEGFLVFKQPwpGIMRTVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEV 565
Cdd:cd17642 373 GKTLGPNERGELCVKGP--MIMKGYVNNPEATKALIDKD--GWLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAEL 448
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 566 ESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGhefsKTLQEelkKQVREKIGPIATPDY-----IQHAPGLPKTRS 640
Cdd:cd17642 449 ESILLQHPKIFDAGVAGIPDEDAGELPAAVVVLEAG----KTMTE---KEVMDYVASQVSTAKrlrggVKFVDEVPKGLT 521
|
570
....*....|.
gi 1394780554 641 GKILRRVLRQI 651
Cdd:cd17642 522 GKIDRRKIREI 532
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
116-653 |
4.00e-21 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 97.64 E-value: 4.00e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFAS-VLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVT 194
Cdd:PRK08751 50 TITYREADQLVEQFAAyLLGELQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVV 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 195 ADAFyrGDKLISLkqIADDALQKC----------KEKGFLVRncIVVNHlgreelvsdgttsqsppVKRLCQDVQMPWNs 264
Cdd:PRK08751 130 IDNF--GTTVQQV--IADTPVKQVittglgdmlgFPKAALVN--FVVKY-----------------VKKLVPEYRINGA- 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 265 dvdLWWHELMRNASKECEPEW-CDSEDELFILYTSGSTGKPKGVVhtvggymiftaATFKYVFDYHPEDIYWCTGDIGWI 343
Cdd:PRK08751 186 ---IRFREALALGRKHSMPTLqIEPDDIAFLQYTGGTTGVAKGAM-----------LTHRNLVANMQQAHQWLAGTGKLE 251
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 344 TGHS-YLTYGPL-------ANGATSVLFEGVPTYPDVSR-MWNIVDKYKVTKlYTSPTAIRALMKS--GDEPVKKCSRES 412
Cdd:PRK08751 252 EGCEvVITALPLyhifaltANGLVFMKIGGCNHLISNPRdMPGFVKELKKTR-FTAFTGVNTLFNGllNTPGFDQIDFSS 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 413 LKIL---GTVGEPINPEAWLWYYHVigdercPIVDTFWQTETgghmlTPLPAVTAMK----PGSATFPFFGVVPAILDES 485
Cdd:PRK08751 331 LKMTlggGMAVQRSVAERWKQVTGL------TLVEAYGLTET-----SPAACINPLTlkeyNGSIGLPIPSTDACIKDDA 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 486 GQELEGEAEGFLVFKQPwpGIMRtvyGNYKRF-ETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAE 564
Cdd:PRK08751 400 GTVLAIGEIGELCIKGP--QVMK---GYWKRPeETAKVMDADGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNE 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 565 VESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDghefSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKIL 644
Cdd:PRK08751 475 IEDVIAMMPGVLEVAAVGVPDEKSGEIVKVVIVKKD----PALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKIL 550
|
....*....
gi 1394780554 645 RRVLRQIAK 653
Cdd:PRK08751 551 RRELRDAAK 559
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
292-645 |
6.88e-21 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 94.01 E-value: 6.88e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 292 LFILYTSGSTGKPKGVVHTVGGYMIFTAATFKyVFDYHPEDIYwctgdigwitghsyLTYGPLA-----NGATSVLFEG- 365
Cdd:cd17633 3 FYIGFTSGTTGLPKAYYRSERSWIESFVCNED-LFNISGEDAI--------------LAPGPLShslflYGAISALYLGg 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 366 ---VPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSgDEPVKKcsresLKILGTVGEPINPEAW-----LWYYHVIgd 437
Cdd:cd17633 68 tfiGQRKFNPKSWIRKINQYNATVIYLVPTMLQALART-LEPESK-----IKSIFSSGQKLFESTKkklknIFPKANL-- 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 438 ercpiVDTFWQTETGghMLTPLPAVTAMKPGSATFPFFGVVPAILDESGQELegeaeGFLVFKQPWpgimrtVYGNYKRF 517
Cdd:cd17633 140 -----IEFYGTSELS--FITYNFNQESRPPNSVGRPFPNVEIEIRNADGGEI-----GKIFVKSEM------VFSGYVRG 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 518 EttyFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLycfVT 597
Cdd:cd17633 202 G---FSNPDGWMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIA---VA 275
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|
gi 1394780554 598 LRDGHEFS-KTLQEELKKQV-REKIgpiatPDYIQHAPGLPKTRSGKILR 645
Cdd:cd17633 276 LYSGDKLTyKQLKRFLKQKLsRYEI-----PKKIIFVDSLPYTSSGKIAR 320
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
98-649 |
1.14e-20 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 95.72 E-value: 1.14e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 98 LGDKIAFYWEGNDPGISM-----SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVF 172
Cdd:PRK06145 4 LSASIAFHARRTPDRAALvyrdqEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPIN 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 173 AGFSAESLCERILDSNCSLLVTADAFYrgdklislkqiaddalqkckekgflvrnciVVNHLGREELVSDGTTSQSppVK 252
Cdd:PRK06145 84 YRLAADEVAYILGDAGAKLLLVDEEFD------------------------------AIVALETPKIVIDAAAQAD--SR 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 253 RLCQdvqmpwnsdvdlwwhelmrnASKECEPEWCDSEDELF-ILYTSGSTGKPKGVVHTVGgymiftaatfkyvfdyhpe 331
Cdd:PRK06145 132 RLAQ--------------------GGLEIPPQAAVAPTDLVrLMYTSGTTDRPKGVMHSYG------------------- 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 332 DIYWCTGD----IGWITGHSYLTYGPLAN-GA-----TSVLFEG----VPTYPDVSRMWNIVDKYKVTKLYTSPTAIRAL 397
Cdd:PRK06145 173 NLHWKSIDhviaLGLTASERLLVVGPLYHvGAfdlpgIAVLWVGgtlrIHREFDPEAVLAAIERHRLTCAWMAPVMLSRV 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 398 MKSGDEpvkkcSRESLKILgtvgepinpeAWLwyyhVIGDERCP---------------IVDTFWQTET-GGHMLTPlPA 461
Cdd:PRK06145 253 LTVPDR-----DRFDLDSL----------AWC----IGGGEKTPesrirdftrvftrarYIDAYGLTETcSGDTLME-AG 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 462 VTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPwpgimRTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDG 541
Cdd:PRK06145 313 REIEKIGSTGRALAHVEIRIADGAGRWLPPNMKGEICMRGP-----KVTKGYWKDPEKTAEAFYGDWFRSGDVGYLDEEG 387
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 542 YYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHefSKTLqEELKKQVREKIG 621
Cdd:PRK06145 388 FLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGA--TLTL-EALDRHCRQRLA 464
|
570 580
....*....|....*....|....*...
gi 1394780554 622 PIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:PRK06145 465 SFKVPRQLKVRDELPRNPSGKVLKRVLR 492
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
99-648 |
1.23e-20 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 95.81 E-value: 1.23e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 99 GDKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAE 178
Cdd:cd17646 12 PDAPAVVDEGR------TLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPGYPAD 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 179 SLCERILDSNCSLLVT----ADAFYRGDklislkqiaddalqkckekgflvrnciVVNHLGREELVSDGTTSQSPPVkrl 254
Cdd:cd17646 86 RLAYMLADAGPAVVLTtadlAARLPAGG---------------------------DVALLGDEALAAPPATPPLVPP--- 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 255 cqdvqmpwnsdvdlwwhelmrnaskecepewcDSEDELFILYTSGSTGKPKGVVHTVGG---YMIFTAATFK-------- 323
Cdd:cd17646 136 --------------------------------RPDNLAYVIYTSGSTGRPKGVMVTHAGivnRLLWMQDEYPlgpgdrvl 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 324 ----YVFDYHPEDIYWctgdigwitghsyltygPLANGATSVLFE----GVPTYpdvsrMWNIVDKYKVTKLYTSPTAIR 395
Cdd:cd17646 184 qktpLSFDVSVWELFW-----------------PLVAGARLVVARpgghRDPAY-----LAALIREHGVTTCHFVPSMLR 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 396 ALMKSGDEPvkkcSRESLKILGTVGEPINPEAWLWYYHVIGderCPIVDTFWQTET----------GGHMLTPLPAvtam 465
Cdd:cd17646 242 VFLAEPAAG----SCASLRRVFCSGEALPPELAARFLALPG---AELHNLYGPTEAaidvthwpvrGPAETPSVPI---- 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 466 kpGSatfPFFGVVPAILDESGQEL----EGE--AEGFLVfkqpwpgimrtVYGNYKRFETTYfKKF------PG--YYVT 531
Cdd:cd17646 311 --GR---PVPNTRLYVLDDALRPVpvgvPGElyLGGVQL-----------ARGYLGRPALTA-ERFvpdpfgPGsrMYRT 373
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 532 GDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEfsKTLQEE 611
Cdd:cd17646 374 GDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARLVGYVVPAAGAA--GPDTAA 451
|
570 580 590
....*....|....*....|....*....|....*..
gi 1394780554 612 LKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd17646 452 LRAHLAERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
289-645 |
1.23e-20 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 93.86 E-value: 1.23e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 289 EDELFILYTSGSTGKPKGVvhtvggyMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLTYGpLANGATSVLFEGV-- 366
Cdd:cd17635 1 EDPLAVIFTSGTTGEPKAV-------LLANKTFFAVPDILQKEGLNWVVGDVTYLPLPATHIGG-LWWILTCLIHGGLcv 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 367 --PTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCsrESLKILGTVGE-PINPEA--WLWYYHVigdercP 441
Cdd:cd17635 73 tgGENTTYKSLFKILTTNAVTTTCLVPTLLSKLVSELKSANATV--PSLRLIGYGGSrAIAADVrfIEATGLT------N 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 442 IVDTFWQTETGGHMLTPLPAvTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPWpgimrTVYGNYKRFETTY 521
Cdd:cd17635 145 TAQVYGLSETGTALCLPTDD-DSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPA-----NMLGYWNNPERTA 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 522 FKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDg 601
Cdd:cd17635 219 EVLIDGWVNTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVASA- 297
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 1394780554 602 hEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILR 645
Cdd:cd17635 298 -ELDENAIRALKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
96-652 |
1.59e-20 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 95.66 E-value: 1.59e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 96 KKLGDKIAFywegndPGISMSITYRQLLQDVCQFASVLQEQ-GIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAG 174
Cdd:PRK12492 35 KKFADRPAF------SNLGVTLSYAELERHSAAFAAYLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPL 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 175 FSAESLCERILDSNCSLLVTADAFyrgDKLISlKQIADDALQKCKE----------KGFLVRncIVVNHLgrEELVSDGT 244
Cdd:PRK12492 109 YTAREMRHQFKDSGARALVYLNMF---GKLVQ-EVLPDTGIEYLIEakmgdllpaaKGWLVN--TVVDKV--KKMVPAYH 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 245 TSQSPPVKRLcqdvqmpwnsdvdlwwheLMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIftaatfky 324
Cdd:PRK12492 181 LPQAVPFKQA------------------LRQGRGLSLKPVPVGLDDIAVLQYTGGTTGLAKGAMLTHGNLVA-------- 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 325 vfdyHPEDIYWCTGDIGwITGHS---------------YLTYGPLAN-------GATSVLFegvpTYP-DVSRMWNIVDK 381
Cdd:PRK12492 235 ----NMLQVRACLSQLG-PDGQPlmkegqevmiaplplYHIYAFTANcmcmmvsGNHNVLI----TNPrDIPGFIKELGK 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 382 YKVTKLYTSPTAIRALMksgDEP-VKKCSRESLKILGTVGEPI---NPEAWlwyYHVIGderCPIVDTFWQTETGGhMLT 457
Cdd:PRK12492 306 WRFSALLGLNTLFVALM---DHPgFKDLDFSALKLTNSGGTALvkaTAERW---EQLTG---CTIVEGYGLTETSP-VAS 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 458 PLPAVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPwpGIMRtvyGNYKRFETTY-FKKFPGYYVTGDGCR 536
Cdd:PRK12492 376 TNPYGELARLGTVGIPVPGTALKVIDDDGNELPLGERGELCIKGP--QVMK---GYWQQPEATAeALDAEGWFKTGDIAV 450
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 537 RDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGhefsKTLQEELKKQV 616
Cdd:PRK12492 451 IDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVVARDP----GLSVEELKAYC 526
|
570 580 590
....*....|....*....|....*....|....*.
gi 1394780554 617 REKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIA 652
Cdd:PRK12492 527 KENFTGYKVPKHIVLRDSLPMTPVGKILRRELRDIA 562
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
288-642 |
2.73e-20 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 93.22 E-value: 2.73e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 288 SEDELFILYTSGSTGKPKGVV---HTVGGyMIFTAATFKYVfdyHPEDIYWC------TGDIGW------ITGHSYLTYG 352
Cdd:cd05924 2 SADDLYILYTGGTTGMPKGVMwrqEDIFR-MLMGGADFGTG---EFTPSEDAhkaaaaAAGTVMfpapplMHGTGSWTAF 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 353 PLANGATSVLFEGVPTYPDvsRMWNIVDKYKVTKL------YTSPTaIRALMKSGDEPVkkcsrESLKILGTVGEPINPE 426
Cdd:cd05924 78 GGLLGGQTVVLPDDRFDPE--EVWRTIEKHKVTSMtivgdaMARPL-IDALRDAGPYDL-----SSLFAISSGGALLSPE 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 427 awlwyyhvIGDERCP------IVDTFWQTETGGHMLTplpAVTAMKPGSATFPFFGVVPAILDESGQELE--GEAEGFLV 498
Cdd:cd05924 150 --------VKQGLLElvpnitLVDAFGSSETGFTGSG---HSAGSGPETGPFTRANPDTVVLDDDGRVVPpgSGGVGWIA 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 499 FKQPWPgimrtvYGNYKRFETT--YFKKFPG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKA 574
Cdd:cd05924 219 RRGHIP------LGYYGDEAKTaeTFPEVDGvrYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPA 292
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1394780554 575 ISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGK 642
Cdd:cd05924 293 VYDVLVVGRPDERWGQEVVAVVQLREGAGVD---LEELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
96-649 |
1.38e-19 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 92.90 E-value: 1.38e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 96 KKLGDKIAFywegndPGISMSITYRQLLQDVCQFASVLQ-EQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAG 174
Cdd:PRK05677 35 QRFADKPAF------SNLGKTLTYGELYKLSGAFAAWLQqHTDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLIVVNTNPL 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 175 FSAESLCERILDSNCSLLVTadafyrgdklisLKQIADDAlqkckEKgflvrnciVVNHLGREELVSDGTTSQSPPVKRL 254
Cdd:PRK05677 109 YTAREMEHQFNDSGAKALVC------------LANMAHLA-----EK--------VLPKTGVKHVIVTEVADMLPPLKRL 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 255 CQDVQMPWNSDVDLWWH---------ELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHT---VGGYMIFTAATF 322
Cdd:PRK05677 164 LINAVVKHVKKMVPAYHlpqavkfndALAKGAGQPVTEANPQADDVAVLQYTGGTTGVAKGAMLThrnLVANMLQCRALM 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 323 KYVFD------------YHpedIYWCTGDIGWItghsyltygpLANGATSVLfegVPTYPDVSRMWNIVDKYKVTKLYTS 390
Cdd:PRK05677 244 GSNLNegceiliaplplYH---IYAFTFHCMAM----------MLIGNHNIL---ISNPRDLPAMVKELGKWKFSGFVGL 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 391 PTAIRALMKsgDEPVKKCSRESLKILGTVGEPINPEAWLWYYHVIGderCPIVDTFWQTETgghmlTPLPAVT---AMKP 467
Cdd:PRK05677 308 NTLFVALCN--NEAFRKLDFSALKLTLSGGMALQLATAERWKEVTG---CAICEGYGMTET-----SPVVSVNpsqAIQV 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 468 GSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPwpGIMRtvyGNYKRFETT-YFKKFPGYYVTGDGCRRDKDGYYWIT 546
Cdd:PRK05677 378 GTIGIPVPSTLCKVIDDDGNELPLGEVGELCVKGP--QVMK---GYWQRPEATdEILDSDGWLKTGDIALIQEDGYMRIV 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 547 GRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATP 626
Cdd:PRK05677 453 DRKKDMILVSGFNVYPNELEDVLAALPGVLQCAAIGVPDEKSGEAIKVFVVVKPGETLTK---EQVMEHMRANLTGYKVP 529
|
570 580
....*....|....*....|...
gi 1394780554 627 DYIQHAPGLPKTRSGKILRRVLR 649
Cdd:PRK05677 530 KAVEFRDELPTTNVGKILRRELR 552
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
115-650 |
1.40e-19 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 92.64 E-value: 1.40e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 115 MSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALhsivfagfsaeslcerildsncsllvt 194
Cdd:PRK05852 42 IAISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLV--------------------------- 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 195 adafyrgdkLISLKQIADDALQKCKEKGFLVRNCIVVnhlgreelvSDGTTSQSPPVKRlCQDVQMPWNSDVDLWWHELM 274
Cdd:PRK05852 95 ---------VVPLDPALPIAEQRVRSQAAGARVVLID---------ADGPHDRAEPTTR-WWPLTVNVGGDSGPSGGTLS 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 275 RNASKECEPEWCDS------EDELFILYTSGSTGKPKGV----------VHT-VGGYMIFTA-ATFKYVFDYHpediywc 336
Cdd:PRK05852 156 VHLDAATEPTPATStpeglrPDDAMIMFTGGTTGLPKMVpwthaniassVRAiITGYRLSPRdATVAVMPLYH------- 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 337 tgdigwitGHSYLT--YGPLANGATSVLfegvPTYPDVS--RMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRES 412
Cdd:PRK05852 229 --------GHGLIAalLATLASGGAVLL----PARGRFSahTFWDDIKAVGATWYTAVPTIHQILLERAATEPSGRKPAA 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 413 LKILGTVGEPINPEAWLWYYHVIGderCPIVDTFWQTET---------GGHMLTPLPAVTAMKPGSATFPFFGVVPAild 483
Cdd:PRK05852 297 LRFIRSCSAPLTAETAQALQTEFA---APVVCAFGMTEAthqvtttqiEGIGQTENPVVSTGLVGRSTGAQIRIVGS--- 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 484 eSGQELEGEAEGflvfkQPW---PGIMRTVYGNYkrfETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLL 560
Cdd:PRK05852 371 -DGLPLPAGAVG-----EVWlrgTTVVRGYLGDP---TITAANFTDGWLRTGDLGSLSAAGDLSIRGRIKELINRGGEKI 441
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 561 STAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRdghEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRS 640
Cdd:PRK05852 442 SPERVEGVLASHPNVMEAAVFGVPDQLYGEAVAAVIVPR---ESAPPTAEELVQFCRERLAAFEIPASFQEASGLPHTAK 518
|
570
....*....|
gi 1394780554 641 GKILRRVLRQ 650
Cdd:PRK05852 519 GSLDRRAVAE 528
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
294-649 |
2.11e-19 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 90.03 E-value: 2.11e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 294 ILYTSGSTGKPKGVVHT----------VGGYMIFTAATFKYVfdyhPEDIYWCTGDIgwITGHSYLTYGplangATSVLF 363
Cdd:cd05917 7 IQFTSGTTGSPKGATLThhnivnngyfIGERLGLTEQDRLCI----PVPLFHCFGSV--LGVLACLTHG-----ATMVFP 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 364 EgvPTYpDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGtvGEPINPEAWLWYYHVIGDERCPIV 443
Cdd:cd05917 76 S--PSF-DPLAVLEAIEKEKCTALHGVPTMFIAELEHPDFDKFDLSSLRTGIMA--GAPCPPELMKRVIEVMNMKDVTIA 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 444 dtFWQTETGghmltplPAVTAMKPGSA----------TFPFFGVvpAILDESGQEL-----EGE--AEGFLVfkqpwpgi 506
Cdd:cd05917 151 --YGMTETS-------PVSTQTRTDDSiekrvntvgrIMPHTEA--KIVDPEGGIVppvgvPGElcIRGYSV-------- 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 507 MRTVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHP 586
Cdd:cd05917 212 MKGYWNDPEKTAEAIDGD--GWLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDE 289
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1394780554 587 VKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:cd05917 290 RYGEEVCAWIRLKEGAELTE---EDIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
287-653 |
2.52e-19 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 92.00 E-value: 2.52e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVV--HTvGGYMIFTAATFKYVFDYHPedIYWCTGDI----GWItghsyLTYGPLANGATS 360
Cdd:PLN03102 184 DEHDPISLNYTSGTTADPKGVVisHR-GAYLSTLSAIIGWEMGTCP--VYLWTLPMfhcnGWT-----FTWGTAARGGTS 255
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 361 VLFEGVpTYPDVSRmwNIvDKYKVTKLYTSPTAIRALMKsGDEPVKKCSRESLKILgTVGEPiNPEAWLWYYHVIGDErc 440
Cdd:PLN03102 256 VCMRHV-TAPEIYK--NI-EMHNVTHMCCVPTVFNILLK-GNSLDLSPRSGPVHVL-TGGSP-PPAALVKKVQRLGFQ-- 326
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 441 pIVDTFWQTETGGHML--------TPLPAVTAM----KPGSATFPFFGVvpAILDESGQE---LEGEAEGFLVFKQPwpG 505
Cdd:PLN03102 327 -VMHAYGLTEATGPVLfcewqdewNRLPENQQMelkaRQGVSILGLADV--DVKNKETQEsvpRDGKTMGEIVIKGS--S 401
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 506 IMRtvyGNYKRFETTY--FKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSH 583
Cdd:PLN03102 402 IMK---GYLKNPKATSeaFKH--GWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAM 476
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1394780554 584 PHPVKGECLYCFVTLRDGHEFSK-------TLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAK 653
Cdd:PLN03102 477 PHPTWGETPCAFVVLEKGETTKEdrvdklvTRERDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLRDIAK 553
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
88-649 |
3.14e-19 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 92.40 E-value: 3.14e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 88 LLDRNVNEKKLGDKIAFYwegnDPGIsmsITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGAL 167
Cdd:PRK06060 9 LLAEQASEAGWYDRPAFY----AADV---VTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVM 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 168 HSIVFAGFSAESLCERILDSNCSLLVTADAFYrgDKLISlKQIADDAlqkckekgflvrncivvnhlgreELVSDGTtsq 247
Cdd:PRK06060 82 AFLANPELHRDDHALAARNTEPALVVTSDALR--DRFQP-SRVAEAA-----------------------ELMSEAA--- 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 248 sppvkrlcqdvqmpwnsdvdlwwhelmRNASKECEPEWCDSEdeLFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFD 327
Cdd:PRK06060 133 ---------------------------RVAPGGYEPMGGDAL--AYATYTSGTTGPPKAAIHRHADPLTFVDAMCRKALR 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 328 YHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLfEGVPTYPDVSRMwnIVDKYKVTKLYTSPTAIRALmksgdepVKK 407
Cdd:PRK06060 184 LTPEDTGLCSARMYFAYGLGNSVWFPLATGGSAVI-NSAPVTPEAAAI--LSARFGPSVLYGVPNFFARV-------IDS 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 408 CSRES---LKILGTVGEPINP---EAWLWYYHVIgdercPIVDTFWQTETGGHMLTPlpAVTAMKPGS--ATFPFFGVvp 479
Cdd:PRK06060 254 CSPDSfrsLRCVVSAGEALELglaERLMEFFGGI-----PILDGIGSTEVGQTFVSN--RVDEWRLGTlgRVLPPYEI-- 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 480 AILDESGQELEGEAEGFLVFKQPwpgimrTVYGNYKRFETTYFKKfPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHL 559
Cdd:PRK06060 325 RVVAPDGTTAGPGVEGDLWVRGP------AIAKGYWNRPDSPVAN-EGWLDTRDRVCIDSDGWVTYRCRADDTEVIGGVN 397
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 560 LSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTR 639
Cdd:PRK06060 398 VDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDGSVMRDLHRGLLNRLSAFKVPHRFAVVDRLPRTP 477
|
570
....*....|
gi 1394780554 640 SGKILRRVLR 649
Cdd:PRK06060 478 NGKLVRGALR 487
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
117-650 |
3.24e-19 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 93.10 E-value: 3.24e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTAD 196
Cdd:PRK12316 4577 LTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPLDPEYPRERLAYMMEDSGAALLLTQS 4656
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 afyrgdKLISLKQIADDAlqkckekgflvrNCIVVNHLGREElvsdGTTSQSPPVKrlcqdvqmpwnsdvdlwwhelmrn 276
Cdd:PRK12316 4657 ------HLLQRLPIPDGL------------ASLALDRDEDWE----GFPAHDPAVR------------------------ 4690
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 askecepewCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYvFDYHPEDIYWCTGDIGWITGHSYLtYGPLAN 356
Cdd:PRK12316 4691 ---------LHPDNLAYVIYTSGSTGRPKGVAVSHGSLVNHLHATGER-YELTPDDRVLQFMSFSFDGSHEGL-YHPLIN 4759
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 357 GAtSVLFEGvPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKsGDEPVKKCSRESLKILGtvGEPINPEA---WLWYYH 433
Cdd:PRK12316 4760 GA-SVVIRD-DSLWDPERLYAEIHEHRVTVLVFPPVYLQQLAE-HAERDGEPPSLRVYCFG--GEAVAQASydlAWRALK 4834
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 434 VIGdercpIVDTFWQTETgghMLTPL--PAVTAMKPGSATFPFFGVVPA----ILDESGQ--------EL----EGEAEG 495
Cdd:PRK12316 4835 PVY-----LFNGYGPTET---TVTVLlwKARDGDACGAAYMPIGTPLGNrsgyVLDGQLNplpvgvagELylggEGVARG 4906
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 496 FLvfkqPWPGImrtvygNYKRFETTYFKKfPG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHK 573
Cdd:PRK12316 4907 YL----ERPAL------TAERFVPDPFGA-PGgrLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHP 4975
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 574 AISEAAVVSHPHPVkGECLYCFVT-----LRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:PRK12316 4976 AVREAVVIAQEGAV-GKQLVGYVVpqdpaLADADEAQAELRDELKAALRERLPEYMVPAHLVFLARMPLTPNGKLDRKAL 5054
|
..
gi 1394780554 649 RQ 650
Cdd:PRK12316 5055 PQ 5056
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
294-649 |
5.72e-19 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 90.45 E-value: 5.72e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 294 ILYTSGSTGKPKGV--------VHTVGGYMIFTAATFkyvFDYHPEDIYWCTGDIGwitgHSyltyGPL-------ANGA 358
Cdd:PRK13390 153 MLYSSGTTGFPKGIqpdlpgrdVDAPGDPIVAIARAF---YDISESDIYYSSAPIY----HA----APLrwcsmvhALGG 221
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 359 TSVLFEGVptypDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEPINPEAwlwyYHVIGDE 438
Cdd:PRK13390 222 TVVLAKRF----DAQATLGHVERYRITVTQMVPTMFVRLLKLDADVRTRYDVSSLRAVIHAAAPCPVDV----KHAMIDW 293
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 439 RCPIVDTFWQ-TETGGHMLTPLPAVTAmKPGSATFPFFGVVpAILDESGQELEGEAEGFLVFKQPwpgimRTVYGNYKRF 517
Cdd:PRK13390 294 LGPIVYEYYSsTEAHGMTFIDSPDWLA-HPGSVGRSVLGDL-HICDDDGNELPAGRIGTVYFERD-----RLPFRYLNDP 366
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 518 ETTYFKKFPG---YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYC 594
Cdd:PRK13390 367 EKTAAAQHPAhpfWTTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKA 446
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*
gi 1394780554 595 FVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:PRK13390 447 VIQLVEGIRGSDELARELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
117-648 |
7.24e-19 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 92.15 E-value: 7.24e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTAD 196
Cdd:PRK12467 538 LSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQDRLAYMLDDSGVRLLLTQS 617
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 afyrgdklislkqiaddalqkckekgflvrncivvnHLGREELVSDGTTSqsppvkrlcqdvqMPWNSDVDLWWHELMRN 276
Cdd:PRK12467 618 ------------------------------------HLLAQLPVPAGLRS-------------LCLDEPADLLCGYSGHN 648
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 ASKECEPEwcdseDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYvFDYHPEDIYWCTGDIGWITGHSYLtYGPLAN 356
Cdd:PRK12467 649 PEVALDPD-----NLAYVIYTSGSTGQPKGVAISHGALANYVCVIAER-LQLAADDSMLMVSTFAFDLGVTEL-FGALAS 721
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 357 GATsVLFEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKsgDEPVKKCSRESLKILGtvGEpINPEAWLWYYHVIG 436
Cdd:PRK12467 722 GAT-LHLLPPDCARDAEAFAALMADQGVTVLKIVPSHLQALLQ--ASRVALPRPQRALVCG--GE-ALQVDLLARVRALG 795
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 437 DErCPIVDTFWQTETGGHM----LTPLPAVTAMKP-GSatfPFFGVVPAILDESGQELEGEAEGFLVFKQPwpGIMRTVY 511
Cdd:PRK12467 796 PG-ARLINHYGPTETTVGVstyeLSDEERDFGNVPiGQ---PLANLGLYILDHYLNPVPVGVVGELYIGGA--GLARGYH 869
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 512 G----NYKRFETTYFKKfPG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPH 585
Cdd:PRK12467 870 RrpalTAERFVPDPFGA-DGgrLYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLAQPG 948
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1394780554 586 PVKGECL-YCFVTLRDGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:PRK12467 949 DAGLQLVaYLVPAAVADGAEHQATRDELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKAL 1012
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
287-648 |
9.74e-19 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 89.45 E-value: 9.74e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVVHTvggYMIFTAATFKYVFDYHPE--------------DIYwcTGDIGWitghsyltyg 352
Cdd:cd17650 91 QPEDLAYVIYTSGTTGKPKGVMVE---HRNVAHAAHAWRREYELDsfpvrllqmasfsfDVF--AGDFAR---------- 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 353 PLANGATSVLfegVP--TYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEPINPEAWLw 430
Cdd:cd17650 156 SLLNGGTLVI---CPdeVKLDPAALYDLILKSRITLMESTPALIRPVMAYVYRNGLDLSAMRLLIVGSDGCKAQDFKTL- 231
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 431 yYHVIGdERCPIVDTFWQTE----TGGHMLTPLPAVT-AMKP-GSatfPFFGVVPAILDESGQ--------EL----EGE 492
Cdd:cd17650 232 -AARFG-QGMRIINSYGVTEatidSTYYEEGRDPLGDsANVPiGR---PLPNTAMYVLDERLQpqpvgvagELyiggAGV 306
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 493 AEGFLvfKQPWPGIMRtvygnykrFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEH 572
Cdd:cd17650 307 ARGYL--NRPELTAER--------FVENPFAPGERMYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARH 376
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1394780554 573 KAISEAAVVSHpHPVKGE---CLYCFVTlrdghefSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd17650 377 PAIDEAVVAVR-EDKGGEarlCAYVVAA-------ATLNTAELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
122-650 |
1.53e-18 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 89.37 E-value: 1.53e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 122 LLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVT-ADAfyr 200
Cdd:PRK12406 17 LAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLIAhADL--- 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 201 gdklisLKQIADDALQKCKekgflvrncivvnhlgreeLVSDGTTSQSPPVKRLCQDVQMPWNSDVDLwwhelmrnaske 280
Cdd:PRK12406 94 ------LHGLASALPAGVT-------------------VLSVPTPPEIAAAYRISPALLTPPAGAIDW------------ 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 281 cePEWCD---------SEDELFILYTSGSTGKPKGVVHT--------VGGYMIFTAATFKyvfdyhPEDIYWCTGDIGwi 343
Cdd:PRK12406 137 --EGWLAqqepydgppVPQPQSMIYTSGTTGHPKGVRRAaptpeqaaAAEQMRALIYGLK------PGIRALLTGPLY-- 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 344 tgHSyltyGPLANGATSVLFEGV----PTYpDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTV 419
Cdd:PRK12406 207 --HS----APNAYGLRAGRLGGVlvlqPRF-DPEELLQLIERHRITHMHMVPTMFIRLLKLPEEVRAKYDVSSLRHVIHA 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 420 GEPINPE------AWlWyyhvigderCPIVDTFW-QTETGghmltplpAVTAM-------KPGSATFPFFGVVPAILDES 485
Cdd:PRK12406 280 AAPCPADvkramiEW-W---------GPVIYEYYgSTESG--------AVTFAtsedalsHPGTVGKAAPGAELRFVDED 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 486 GQELEGEAEGFLVFKQPwpGIMRTVYGNY--KRFETtyfkKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTA 563
Cdd:PRK12406 342 GRPLPQGEIGEIYSRIA--GNPDFTYHNKpeKRAEI----DRGGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPA 415
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 564 EVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKI 643
Cdd:PRK12406 416 EIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEPQPGATLD---EADIRAQLKARLAGYKVPKHIEIMAELPREDSGKI 492
|
....*..
gi 1394780554 644 LRRVLRQ 650
Cdd:PRK12406 493 FKRRLRD 499
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
96-648 |
2.61e-18 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 88.54 E-value: 2.61e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 96 KKLGDKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGF 175
Cdd:cd17655 8 EKTPDHTAVVFEDQ------TLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPDY 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 176 SAESLcERIL-DSNCSLLVTADAFyrgDKLIslkqiaddalqkckekgflvrncivvNHLGREELVSDGTTSqsppvkrl 254
Cdd:cd17655 82 PEERI-QYILeDSGADILLTQSHL---QPPI--------------------------AFIGLIDLLDEDTIY-------- 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 255 cqdvqmpwnsdvdlwwHElmrnASKECEPEwCDSEDELFILYTSGSTGKPKGV----------VHTVGGYMIFTAAT--- 321
Cdd:cd17655 124 ----------------HE----ESENLEPV-SKSDDLAYVIYTSGSTGKPKGVmiehrgvvnlVEWANKVIYQGEHLrva 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 322 --FKYVFDYHPEDIYwctgdigwitghsyltyGPLANGATSVLFEGVpTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMK 399
Cdd:cd17655 183 lfASISFDASVTEIF-----------------ASLLSGNTLYIVRKE-TVLDGQALTQYIRQNRITIIDLTPAHLKLLDA 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 400 SGDEPvkkcsRESLKILGTVGEPINPE-AWLWYYHVigDERCPIVDTFWQTETG-GHMLTPlpaVTAMKPGSATFPFfGV 477
Cdd:cd17655 245 ADDSE-----GLSLKHLIVGGEALSTElAKKIIELF--GTNPTITNAYGPTETTvDASIYQ---YEPETDQQVSVPI-GK 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 478 vPA------ILDESGQ--------EL----EGEAEGFLvfkqpwpgimrtvygnyKRFETTYfKKF------PG--YYVT 531
Cdd:cd17655 314 -PLgntriyILDQYGRpqpvgvagELyiggEGVARGYL-----------------NRPELTA-EKFvddpfvPGerMYRT 374
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 532 GDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTlRDGHEFSKTLQEE 611
Cdd:cd17655 375 GDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIV-SEKELPVAQLREF 453
|
570 580 590
....*....|....*....|....*....|....*..
gi 1394780554 612 LKKQVREKIgpiaTPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd17655 454 LARELPDYM----IPSYFIKLDEIPLTPNGKVDRKAL 486
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
117-668 |
2.88e-18 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 90.02 E-value: 2.88e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTAD 196
Cdd:PRK12316 3083 LSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLLLSQS 3162
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 afyrgdklislkqiaddalqkckekgflvrncivvnHLgreelvsdgTTSQSPPVKRLCQDVQMpwnsdvdlwwhelmRN 276
Cdd:PRK12316 3163 ------------------------------------HL---------RLPLAQGVQVLDLDRGD--------------EN 3183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 ASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKyVFDYHPEDIYWCTGDIGWiTGHSYLTYGPLAN 356
Cdd:PRK12316 3184 YAEANPAIRTMPENLAYVIYTSGSTGKPKGVGIRHSALSNHLCWMQQ-AYGLGVGDRVLQFTTFSF-DVFVEELFWPLMS 3261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 357 GATSVLfEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMksgdEPVKKCSRESLKILGTVGEPINPE------AWLW 430
Cdd:PRK12316 3262 GARVVL-AGPEDWRDPALLVELINSEGVDVLHAYPSMLQAFL----EEEDAHRCTSLKRIVCGGEALPADlqqqvfAGLP 3336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 431 YYHVIGDERCPIVDTFWQTETGGHMLTPlpaVTAMKPGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPWPGImrtv 510
Cdd:PRK12316 3337 LYNLYGPTEATITVTHWQCVEEGKDAVP---IGRPIANRACYILDGSLEPVPVGALGELYLGGEGLARGYHNRPGL---- 3409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 511 ygNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVShphpVKGE 590
Cdd:PRK12316 3410 --TAERFVPDPFVPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVLA----VDGR 3483
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 591 CLYCFVTLRDGhefSKTLQEELKKQVREKIgpiatPDYIQHA-----PGLPKTRSGKILRRVLrqiAKNDRDLGDTSTVA 665
Cdd:PRK12316 3484 QLVAYVVPEDE---AGDLREALKAHLKASL-----PEYMVPAhllflERMPLTPNGKLDRKAL---PRPDAALLQQDYVA 3552
|
...
gi 1394780554 666 DQS 668
Cdd:PRK12316 3553 PVN 3555
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
290-645 |
3.94e-18 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 86.17 E-value: 3.94e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 290 DELFILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSyLTYGPLANGATSVLFEGVpty 369
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGN-LIAANLQLIHAMGLTEADVYLNMLPLFHIAGLN-LALATFHAGGANVVMEKF--- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 370 pDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSreSLKILGTVGEPINPEAWLwyyhvigdERCPivDTFW-- 447
Cdd:cd17637 76 -DPAEALELIEEEKVTLMGSFPPILSNLLDAAEKSGVDLS--SLRHVLGLDAPETIQRFE--------ETTG--ATFWsl 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 448 --QTETGGhMLTPLPAVTamKPGSATFPFFGVVPAILDESGQELE----GE--AEGFLVFKQPWpgimrtvygNYKRFET 519
Cdd:cd17637 143 ygQTETSG-LVTLSPYRE--RPGSAGRPGPLVRVRIVDDNDRPVPagetGEivVRGPLVFQGYW---------NLPELTA 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 520 TYFKKfpGYYVTGDGCRRDKDGYYWITGRI--DDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVT 597
Cdd:cd17637 211 YTFRN--GWHHTGDLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCV 288
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 1394780554 598 LRDGHEFSKtlqEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILR 645
Cdd:cd17637 289 LKPGATLTA---DELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
116-648 |
1.30e-17 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 86.17 E-value: 1.30e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLcERIL-DSNCSLLVT 194
Cdd:cd12114 12 TLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARR-EAILaDAGARLVLT 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 195 ADAfyrgdklislkqiaddalqkckekgfLVRNCIVVNHLGREELVSDGTTSQSPPVKRlcqdvqmpwnsdvdlwwhelm 274
Cdd:cd12114 91 DGP--------------------------DAQLDVAVFDVLILDLDALAAPAPPPPVDV--------------------- 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 275 rnaskecepewcDSEDELFILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCtgdigwITGHS-----YL 349
Cdd:cd12114 124 ------------APDDLAYVIFTSGSTGTPKGVMISHRA-ALNTILDINRRFAVGPDDRVLA------LSSLSfdlsvYD 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 350 TYGPLANGATSVLfegvPTY---PDVSRMWNIVDKYKVTkLYTSPTAI------------------RALMKSGDE-PVKK 407
Cdd:cd12114 185 IFGALSAGATLVL----PDEarrRDPAHWAELIERHGVT-LWNSVPALlemlldvleaaqallpslRLVLLSGDWiPLDL 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 408 CSResLKILGTVGEPIN----PEAWLW-YYHVIGDercpiVDTFWQTETGGHmltPLPAVTAmkpgsatfpffgvvpAIL 482
Cdd:cd12114 260 PAR--LRALAPDARLISlggaTEASIWsIYHPIDE-----VPPDWRSIPYGR---PLANQRY---------------RVL 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 483 DESGQEL----EGE--AEGflvfkqpwPGIMRTVYGNYKRFETTYFKKFPG--YYVTGD-GCRRDkDGYYWITGRIDDML 553
Cdd:cd12114 315 DPRGRDCpdwvPGElwIGG--------RGVALGYLGDPELTAARFVTHPDGerLYRTGDlGRYRP-DGTLEFLGRRDGQV 385
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 554 NVSGHLLSTAEVESALAEHKAISEAAVVSHPHPvKGECLYCFVTLRDGHE--FSKTLQEELKKQVREKIGPiatPDYIQh 631
Cdd:cd12114 386 KVRGYRIELGEIEAALQAHPGVARAVVVVLGDP-GGKRLAAFVVPDNDGTpiAPDALRAFLAQTLPAYMIP---SRVIA- 460
|
570
....*....|....*..
gi 1394780554 632 APGLPKTRSGKILRRVL 648
Cdd:cd12114 461 LEALPLTANGKVDRAAL 477
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
114-653 |
1.85e-17 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 86.05 E-value: 1.85e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 114 SMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLV 193
Cdd:PLN02479 43 SVRYTWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHFGVPMAGAVVNCVNIRLNAPTIAFLLEHSKSEVVM 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 194 TADAFYrgdklislkQIADDALQKCKEKGflvrncivVNHLGREELVSDGTTSQSPpvKRLCqdvqmpwnsdvdlwwHEL 273
Cdd:PLN02479 123 VDQEFF---------TLAEEALKILAEKK--------KSSFKPPLLIVIGDPTCDP--KSLQ---------------YAL 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 274 MRNAS------KECEPE--WCDSEDE---LFILYTSGSTGKPKGVV-HTVGGYMIftAATFKYVFDYHPEDIYWCTGDIG 341
Cdd:PLN02479 169 GKGAIeyekflETGDPEfaWKPPADEwqsIALGYTSGTTASPKGVVlHHRGAYLM--ALSNALIWGMNEGAVYLWTLPMF 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 342 WITGHSYlTYGPLANGATSVLFEGVPTypdvSRMWNIVDKYKVTKLYTSPTAIRAL------------------MKSGDE 403
Cdd:PLN02479 247 HCNGWCF-TWTLAALCGTNICLRQVTA----KAIYSAIANYGVTHFCAAPVVLNTIvnapksetilplprvvhvMTAGAA 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 404 P----VKKCSRESLKILGTVG--EPINPE---AWL--WyyhvigDERCPIVDTFWQTETGGHM--LTPLPAV--TAMKPg 468
Cdd:PLN02479 322 PppsvLFAMSEKGFRVTHTYGlsETYGPStvcAWKpeW------DSLPPEEQARLNARQGVRYigLEGLDVVdtKTMKP- 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 469 satfpffgvVPAildesgqelEGEAEGFLVFKQPwpGIMRTVYGNYKRFETTYFKkfpGYYVTGDGCRRDKDGYYWITGR 548
Cdd:PLN02479 395 ---------VPA---------DGKTMGEIVMRGN--MVMKGYLKNPKANEEAFAN---GWFHSGDLGVKHPDGYIEIKDR 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 549 IDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFS--KTLQEELKKQVREKIGPIATP 626
Cdd:PLN02479 452 SKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESPCAFVTLKPGVDKSdeAALAEDIMKFCRERLPAYWVP 531
|
570 580
....*....|....*....|....*..
gi 1394780554 627 DYIQHAPgLPKTRSGKILRRVLRQIAK 653
Cdd:PLN02479 532 KSVVFGP-LPKTATGKIQKHVLRAKAK 557
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
117-648 |
2.34e-17 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 87.14 E-value: 2.34e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTAd 196
Cdd:PRK12467 1600 LTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQ- 1678
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 afyrgdklislkqiaddalqkckekgflvrncivvNHLGREELVSDGttsqsppVKRLCQDVQMPWNSdvdlwwhelmrn 276
Cdd:PRK12467 1679 -----------------------------------SHLQARLPLPDG-------LRSLVLDQEDDWLE------------ 1704
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 ASKECEPEWCDSEDEL-FILYTSGSTGKPKGVVHTVGGYMIFTAATfKYVFDYHPEDIywctgdigWITGHSYL------ 349
Cdd:PRK12467 1705 GYSDSNPAVNLAPQNLaYVIYTSGSTGRPKGAGNRHGALVNRLCAT-QEAYQLSAADV--------VLQFTSFAfdvsvw 1775
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 350 -TYGPLANGATSVLfegVP--TYPDVSRMWNIVDKYKVTKLYTSPTAIRALMkSGDEPVKKCSreSLKILGTVGEPINPE 426
Cdd:PRK12467 1776 eLFWPLINGARLVI---APpgAHRDPEQLIQLIERQQVTTLHFVPSMLQQLL-QMDEQVEHPL--SLRRVVCGGEALEVE 1849
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 427 AW-LWYyhvigdERCP---IVDTFWQTETGGHmLTPLPAVTAMKPGSATFPFfGVVPA-----ILDES--------GQEL 489
Cdd:PRK12467 1850 ALrPWL------ERLPdtgLFNLYGPTETAVD-VTHWTCRRKDLEGRDSVPI-GQPIAnlstyILDASlnpvpigvAGEL 1921
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 490 ----EGEAEGFLvfkqPWPGIMRTvygnykRFETTYFKKFPG-YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAE 564
Cdd:PRK12467 1922 ylggVGLARGYL----NRPALTAE------RFVADPFGTVGSrLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGE 1991
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 565 VESALAEHKAISEAAVVSHPHPvKGECLYCFVT-----LRDGHEFSKTLQEELKKQVREKIgpiatPDYI--QH---APG 634
Cdd:PRK12467 1992 IEARLREQGGVREAVVIAQDGA-NGKQLVAYVVptdpgLVDDDEAQVALRAILKNHLKASL-----PEYMvpAHlvfLAR 2065
|
570
....*....|....
gi 1394780554 635 LPKTRSGKILRRVL 648
Cdd:PRK12467 2066 MPLTPNGKLDRKAL 2079
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
296-654 |
2.82e-17 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 85.57 E-value: 2.82e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 296 YTSGSTGKPKGVVHTvggymiftaatfkyvfdyHPEDIYwctgdigwitgHSYLTYGPLANGATS--VLFEGVPTY---- 369
Cdd:PRK06018 184 YTSGTTGDPKGVLYS------------------HRSNVL-----------HALMANNGDALGTSAadTMLPVVPLFhans 234
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 370 ----------------P----DVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSResLKILGTVGEPInPEAWL 429
Cdd:PRK06018 235 wgiafsapsmgtklvmPgaklDGASVYELLDTEKVTFTAGVPTVWLMLLQYMEKEGLKLPH--LKMVVCGGSAM-PRSMI 311
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 430 WYYHVIGDErcpIVDTFWQTETgghmlTPLPAVTAMKPGSAT--------------FPFFGVVPAILDESGQEL--EGEA 493
Cdd:PRK06018 312 KAFEDMGVE---VRHAWGMTEM-----SPLGTLAALKPPFSKlpgdarldvlqkqgYPPFGVEMKITDDAGKELpwDGKT 383
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 494 EGFLVFKQPwpgimrTVYGNYKRFETTYFKKfPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHK 573
Cdd:PRK06018 384 FGRLKVRGP------AVAAAYYRVDGEILDD-DGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHP 456
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 574 AISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAK 653
Cdd:PRK06018 457 KVAEAAVIGVYHPKWDERPLLIVQLKPGETATR---EEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALREQFK 533
|
.
gi 1394780554 654 N 654
Cdd:PRK06018 534 D 534
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
287-650 |
5.47e-17 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 84.40 E-value: 5.47e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVVHT-VGGYMIFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYLtYGPLANGATSVLFEG 365
Cdd:cd05915 151 PERAACGMAYTTGTTGLPKGVVYShRALVLHSLAASLVDGTALSEKDVVLPVVPMFHVNAWCLP-YAATLVGAKQVLPGP 229
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 366 VPtypDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEpVKKCSRESLKILGTVGEPinPEAWL-----WYYHVIGDERC 440
Cdd:cd05915 230 RL---DPASLVELFDGEGVTFTAGVPTVWLALADYLES-TGHRLKTLRRLVVGGSAA--PRSLIarferMGVEVRQGYGL 303
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 441 PIV-----DTFWQTEtgghmLTPLPAVTAMK-PGSATFPFFGVVPAILDESGQELEGEAEGFLVFKQPWPGIMRTVYGNY 514
Cdd:cd05915 304 TETspvvvQNFVKSH-----LESLSEEEKLTlKAKTGLPIPLVRLRVADEEGRPVPKDGKALGEVQLKGPWITGGYYGNE 378
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 515 KRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYC 594
Cdd:cd05915 379 EATRSALTPD--GFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLA 456
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*..
gi 1394780554 595 FVTLRDghefSKTLQEELKKQVREKIGPIAT-PDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:cd05915 457 VVVPRG----EKPTPEELNEHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALRE 509
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
116-650 |
1.24e-16 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 82.74 E-value: 1.24e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLcERILD-SNCSLLVT 194
Cdd:cd17653 22 SLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAKLPSARI-QAILRtSGATLLLT 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 195 ADAfyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqdvqmpwnsdvdlwwhelm 274
Cdd:cd17653 101 TDS----------------------------------------------------------------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 275 rnaskecepewcdSEDELFILYTSGSTGKPKGVVHTVGG---YMIFTAAT------------FKYVFDYhpediywCTGD 339
Cdd:cd17653 104 -------------PDDLAYIIFTSGSTGIPKGVMVPHRGvlnYVSQPPARldvgpgsrvaqvLSIAFDA-------CIGE 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 340 IgwitghsyltYGPLANGATSVLFEGVPTYPDVSRmwnivdkyKVTKLYTSPTaIRALMKSGDEPvkkcsreSLKILGTV 419
Cdd:cd17653 164 I----------FSTLCNGGTLVLADPSDPFAHVAR--------TVDALMSTPS-ILSTLSPQDFP-------NLKTIFLG 217
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 420 GEPINP---EAWLW---YYHVIGDERCPIVDTFWQtetgghmLTPLPAVTAMKPgsatFPFFGVVpaILDESGQE-LEGE 492
Cdd:cd17653 218 GEAVPPsllDRWSPgrrLYNAYGPTECTISSTMTE-------LLPGQPVTIGKP----IPNSTCY--ILDADLQPvPEGV 284
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 493 A-EGFLVfkqpWPGIMRTVYGNYKRfETTYFKKFPGY-----YVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVE 566
Cdd:cd17653 285 VgEICIS----GVQVARGYLGNPAL-TASKFVPDPFWpgsrmYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIE 359
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 567 S-ALAEHKAISEAAVVSHphpvkGECLYCFVTlrdghefSKTLQEE-LKKQVREKIGPIATPDYIQHAPGLPKTRSGKIL 644
Cdd:cd17653 360 EvVLQSQPEVTQAAAIVV-----NGRLVAFVT-------PETVDVDgLRSELAKHLPSYAVPDRIIALDSFPLTANGKVD 427
|
....*.
gi 1394780554 645 RRVLRQ 650
Cdd:cd17653 428 RKALRE 433
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
287-653 |
2.23e-16 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 83.43 E-value: 2.23e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVVHTVGGYMIfTAATFKYVFDYHPEDIywctgdigwITG-----HSY----LTYGPLANG 357
Cdd:PRK08633 780 KPDDTATIIFSSGSEGEPKGVMLSHHNILS-NIEQISDVFNLRNDDV---------ILSslpffHSFgltvTLWLPLLEG 849
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 358 ATSVlfegvpTYPD------VSRMwniVDKYKVTKLYTSPTAIRALMKSgdepvKKCSRE---SLKILGTVGEPINPE-- 426
Cdd:PRK08633 850 IKVV------YHPDptdalgIAKL---VAKHRATILLGTPTFLRLYLRN-----KKLHPLmfaSLRLVVAGAEKLKPEva 915
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 427 -AWLWYYHVigdercPIVDTFWQTETgghmlTPLPAV-------------TAMKPGSATFPFFGVVPAILD-ESGQELEG 491
Cdd:PRK08633 916 dAFEEKFGI------RILEGYGATET-----SPVASVnlpdvlaadfkrqTGSKEGSVGMPLPGVAVRIVDpETFEELPP 984
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 492 EAEGFLVFKQPwpGIMRTVYGN-YKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALa 570
Cdd:PRK08633 985 GEDGLILIGGP--QVMKGYLGDpEKTAEVIKDIDGIGWYVTGDKGHLDEDGFLTITDRYSRFAKIGGEMVPLGAVEEEL- 1061
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 571 eHKAISEA----AVVSHPHPVKGECLycfVTLrdgHEFSKTLQEELKKQVRE-KIGPIATPDYIQHAPGLPKTRSGKILR 645
Cdd:PRK08633 1062 -AKALGGEevvfAVTAVPDEKKGEKL---VVL---HTCGAEDVEELKRAIKEsGLPNLWKPSRYFKVEALPLLGSGKLDL 1134
|
....*...
gi 1394780554 646 RVLRQIAK 653
Cdd:PRK08633 1135 KGLKELAL 1142
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
118-653 |
4.14e-16 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 81.78 E-value: 4.14e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGA-LHSIVFAGFSAEslCERILD-SNCSLLVTA 195
Cdd:PRK08315 45 TYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAiLVTINPAYRLSE--LEYALNqSGCKALIAA 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 DAFYRGDKLISLKQIADDaLQKCkEKGFL-------VRNCIvvnHLGREElvSDGTTSqsppvkrlcqdvqmpwnsdvdl 268
Cdd:PRK08315 123 DGFKDSDYVAMLYELAPE-LATC-EPGQLqsarlpeLRRVI---FLGDEK--HPGMLN---------------------- 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 269 wWHELMRNASKECEPEWCDSEDELF------ILYTSGSTGKPKGVV---HTVG--GYMIftAATFKYVfdyhPED----- 332
Cdd:PRK08315 174 -FDELLALGRAVDDAELAARQATLDpddpinIQYTSGTTGFPKGATlthRNILnnGYFI--GEAMKLT----EEDrlcip 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 333 --IYWCtgdIGWITGhsylTYGPLANGATSVL----FE-----------------GVPT-------YPDVSR-------- 374
Cdd:PRK08315 247 vpLYHC---FGMVLG----NLACVTHGATMVYpgegFDplatlaaveeerctalyGVPTmfiaeldHPDFARfdlsslrt 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 375 ------------MWNIVDK---YKVTKLY----TSP----TAIralmksgDEPV-KKCSreslkilgTVGEpINPeawlw 430
Cdd:PRK08315 320 gimagspcpievMKRVIDKmhmSEVTIAYgmteTSPvstqTRT-------DDPLeKRVT--------TVGR-ALP----- 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 431 yyHVigdeRCPIVDTfwqtETGGhmltplpavtamkpgsatfpffgVVPAildesGQEleGE--AEGFLVFKqpwpgimr 508
Cdd:PRK08315 379 --HL----EVKIVDP----ETGE-----------------------TVPR-----GEQ--GElcTRGYSVMK-------- 410
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 509 tvyGNYKRFETTyfKKF---PGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPH 585
Cdd:PRK08315 411 ---GYWNDPEKT--AEAidaDGWMHTGDLAVMDEEGYVNIVGRIKDMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPD 485
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1394780554 586 PVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAK 653
Cdd:PRK08315 486 EKYGEEVCAWIILRPGATLT---EEDVRDFCRGKIAHYKIPRYIRFVDEFPMTVTGKIQKFKMREMMI 550
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
473-653 |
4.50e-16 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 81.68 E-value: 4.50e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 473 PFFGVVPAILDESGQEL--EGEAEGFLVFKQPWpgimrtVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRID 550
Cdd:PRK07008 361 VIYGVDMKIVGDDGRELpwDGKAFGDLQVRGPW------VIDRYFRGDASPLVD--GWFPTGDVATIDADGFMQITDRSK 432
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 551 DMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKtlqEELKKQVREKIGPIATPDYIQ 630
Cdd:PRK07008 433 DVIKSGGEWISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGAEVTR---EELLAFYEGKVAKWWIPDDVV 509
|
170 180
....*....|....*....|...
gi 1394780554 631 HAPGLPKTRSGKILRRVLRQIAK 653
Cdd:PRK07008 510 FVDAIPHTATGKLQKLKLREQFR 532
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
117-650 |
6.11e-16 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 81.08 E-value: 6.11e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGAlhsiVF-----AGFSAEsLCERILDSNCSL 191
Cdd:PRK07514 29 YTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGA----VFlplntAYTLAE-LDYFIGDAEPAL 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 192 LVTADAfyrgdklislkqiADDALQKCKEKgflvRNCIVVNHLGreelvSDGTTSQSppvkrlcqdvqmpwnsdvdlwwh 271
Cdd:PRK07514 104 VVCDPA-------------NFAWLSKIAAA----AGAPHVETLD-----ADGTGSLL----------------------- 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 272 ELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPED-------IYwctgdigwit 344
Cdd:PRK07514 139 EAAAAAPDDFETVPRGADDLAAILYTSGTTGRSKGAMLSHGN-LLSNALTLVDYWRFTPDDvlihalpIF---------- 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 345 gHSY----LTYGPLANGA------------------TSVLFEGVPTYpdvsrmwnivdkYkvTKLYTSPTAIRALmksgd 402
Cdd:PRK07514 208 -HTHglfvATNVALLAGAsmiflpkfdpdavlalmpRATVMMGVPTF------------Y--TRLLQEPRLTREA----- 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 403 epvkkCSRESLKILGTVgePINPEAwlwyyHVIGDERC--PIVDTFWQTETGghMLTPLPAVTAMKPGSATFPFFGVVPA 480
Cdd:PRK07514 268 -----AAHMRLFISGSA--PLLAET-----HREFQERTghAILERYGMTETN--MNTSNPYDGERRAGTVGFPLPGVSLR 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 481 ILD-ESGQELEGEAEGFLVFKQPwpgimrTVYGNYKRF-ETTY--FKKfPGYYVTGDGCRRDKDGYYWITGRIDDMLNVS 556
Cdd:PRK07514 334 VTDpETGAELPPGEIGMIEVKGP------NVFKGYWRMpEKTAeeFRA-DGFFITGDLGKIDERGYVHIVGRGKDLIISG 406
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 557 GHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFS-KTLQEELKKQV-REKIgpiatPDYIQHAPG 634
Cdd:PRK07514 407 GYNVYPKEVEGEIDELPGVVESAVIGVPHPDFGEGVTAVVVPKPGAALDeAAILAALKGRLaRFKQ-----PKRVFFVDE 481
|
570
....*....|....*.
gi 1394780554 635 LPKTRSGKILRRVLRQ 650
Cdd:PRK07514 482 LPRNTMGKVQKNLLRE 497
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
130-649 |
1.65e-15 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 80.02 E-value: 1.65e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 130 ASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTADAFYrgDKLislkq 209
Cdd:PLN02246 64 AAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANPFYTPAEIAKQAKASGAKLIITQSCYV--DKL----- 136
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 210 iaddalqkckeKGFLVRNCIVVnhlgreelvsdgTTSQSPPvkrlcqdvqmpwnsDVDLWWHELMRNASKECEPEWCDSE 289
Cdd:PLN02246 137 -----------KGLAEDDGVTV------------VTIDDPP--------------EGCLHFSELTQADENELPEVEISPD 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 290 DELFILYTSGSTGKPKGVVHTVGGyMIFTAAtfKYV------FDYHPEDIYWCTGDIGWITG-HSYLTYGpLANGATSVL 362
Cdd:PLN02246 180 DVVALPYSSGTTGLPKGVMLTHKG-LVTSVA--QQVdgenpnLYFHSDDVILCVLPMFHIYSlNSVLLCG-LRVGAAILI 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 363 fegVPTYpDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDepVKKCSRESLKILGTVGEPINPEawlwyyhvigdercpI 442
Cdd:PLN02246 256 ---MPKF-EIGALLELIQRHKVTIAPFVPPIVLAIAKSPV--VEKYDLSSIRMVLSGAAPLGKE---------------L 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 443 VDTFW----QTETG-GHMLTPLPAVTAMKPGSATFPF------FGVV-----PAILD-ESGQELEGEAEGFLVFKQPwpG 505
Cdd:PLN02246 315 EDAFRaklpNAVLGqGYGMTEAGPVLAMCLAFAKEPFpvksgsCGTVvrnaeLKIVDpETGASLPRNQPGEICIRGP--Q 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 506 IMRTVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPH 585
Cdd:PLN02246 393 IMKGYLNDPEATANTIDKD--GWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHPSIADAAVVPMKD 470
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 586 PVKGECLYCFVTLRDGHEFSktlQEELK----KQV--REKIGPIATPDYIqhapglPKTRSGKILRRVLR 649
Cdd:PLN02246 471 EVAGEVPVAFVVRSNGSEIT---EDEIKqfvaKQVvfYKRIHKVFFVDSI------PKAPSGKILRKDLR 531
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
99-612 |
2.60e-15 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 79.53 E-value: 2.60e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 99 GDKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALhsivfagfsae 178
Cdd:PRK08279 51 PDRPALLFEDQ------SISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAV----------- 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 179 slcerildsnCSLLVTAdafYRGDKLI-SLKQ------IADDALQKCKEKgflVRNCIvvnHLGREELVSDGTTSQSPPv 251
Cdd:PRK08279 114 ----------VALLNTQ---QRGAVLAhSLNLvdakhlIVGEELVEAFEE---ARADL---ARPPRLWVAGGDTLDDPE- 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 252 krlcqdvqmpwnSDVDLwwHELMRNASKECePEWCDS---EDELFILYTSGSTGKPKGVVHT-------VGGYMIFTAAT 321
Cdd:PRK08279 174 ------------GYEDL--AAAAAGAPTTN-PASRSGvtaKDTAFYIYTSGTTGLPKAAVMShmrwlkaMGGFGGLLRLT 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 322 fkyvfdyhPEDIYWCT--------GDIGWITGhsyltygpLANGATSVL---FEgvptypdVSRMWNIVDKYKVTKLYts 390
Cdd:PRK08279 239 --------PDDVLYCClplyhntgGTVAWSSV--------LAAGATLALrrkFS-------ASRFWDDVRRYRATAFQ-- 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 391 ptAI----RALMksgDEPVKKCSRE-SLKILgtVGEPINPEAWlwyyhvigDErcpivdtfWQTETGGHMLTPLPAVT-- 463
Cdd:PRK08279 294 --YIgelcRYLL---NQPPKPTDRDhRLRLM--IGNGLRPDIW--------DE--------FQQRFGIPRILEFYAASeg 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 464 --AM-----KPGSATF-PFFGVVPAIL---D-ESGQELEGeAEGFLVFKQPW-PGIMRTVYGNYKRFE------------ 518
Cdd:PRK08279 351 nvGFinvfnFDGTVGRvPLWLAHPYAIvkyDvDTGEPVRD-ADGRCIKVKPGeVGLLIGRITDRGPFDgytdpeasekki 429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 519 -TTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDML-----NVsghllSTAEVESALAEHKAISEAAV--VSHPHpVKGE 590
Cdd:PRK08279 430 lRDVFKKGDAWFNTGDLMRDDGFGHAQFVDRLGDTFrwkgeNV-----ATTEVENALSGFPGVEEAVVygVEVPG-TDGR 503
|
570 580
....*....|....*....|....*..
gi 1394780554 591 CLYCFVTLRDGHEF-----SKTLQEEL 612
Cdd:PRK08279 504 AGMAAIVLADGAEFdlaalAAHLYERL 530
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
294-645 |
3.16e-15 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 77.54 E-value: 3.16e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 294 ILYTSGSTGKPKGVV----HTVGGYmiftAAtfkyvfdyhpediyWCtgDIGWIT-GHSYLTYGPLAN------GATSVL 362
Cdd:cd17638 5 IMFTSGTTGRSKGVMcahrQTLRAA----AA--------------WA--DCADLTeDDRYLIINPFFHtfgykaGIVACL 64
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 363 FEGVPTYP----DVSRMWNIVDKYKVTKLYTSPTAIRALMksgDEP-VKKCSRESLKiLGTVGEPINPEAWLWYYHvigd 437
Cdd:cd17638 65 LTGATVVPvavfDVDAILEAIERERITVLPGPPTLFQSLL---DHPgRKKFDLSSLR-AAVTGAATVPVELVRRMR---- 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 438 ERCPIvdtfwQTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAILDesGQELEGEAEGFLVFKQPwpGIMRTVYGNYKRF 517
Cdd:cd17638 137 SELGF-----ETVLTAYGLTEAGVATMCRPGDDAETVATTCGRACP--GFEVRIADDGEVLVRGY--NVMQGYLDDPEAT 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 518 ETTYFKKfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVT 597
Cdd:cd17638 208 AEAIDAD--GWLHTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVV 285
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 1394780554 598 LRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILR 645
Cdd:cd17638 286 ARPGVTLT---EEDVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| ACAS_N |
pfam16177 |
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many ... |
28-87 |
3.77e-15 |
|
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many acetyl-coenzyme A synthetase enzymes.
Pssm-ID: 465043 [Multi-domain] Cd Length: 55 Bit Score: 69.81 E-value: 3.77e-15
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 28 YEKLYRRSVEEPHEFWGDIAKEFYWKShhsgPFlQYNFDVTKGkIFVEWMKGSTTNICYN 87
Cdd:pfam16177 1 YEALYRRSIEDPEGFWGEVAKELDWFK----PF-DKVLDGSNG-PFAKWFVGGKLNVCYN 54
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
270-614 |
5.05e-15 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 78.28 E-value: 5.05e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 270 WHELMRNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMiFTAATFKYVFDYHPEDIYWCTGDIGWITGHSYL 349
Cdd:cd05932 118 WDDLIAQHPPLEERPTRFPEQLATLIYTSGTTGQPKGVMLTFGSFA-WAAQAGIEHIGTEENDRMLSYLPLAHVTERVFV 196
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 350 TYGPLANGATSVLFEGVPTYPD------------VSRMW-----NIVDKYKVTKL--YTSPTAIRALMKSgdEPVKKCSR 410
Cdd:cd05932 197 EGGSLYGGVLVAFAESLDTFVEdvqrarptlffsVPRLWtkfqqGVQDKIPQQKLnlLLKIPVVNSLVKR--KVLKGLGL 274
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 411 ESLKILGTVGEPInPEAWLWYYHVIGderCPIVDTFWQTETGGHMLTPLPAVTamKPGSATFPFFGVvpaildesgqELE 490
Cdd:cd05932 275 DQCRLAGCGSAPV-PPALLEWYRSLG---LNILEAYGMTENFAYSHLNYPGRD--KIGTVGNAGPGV----------EVR 338
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 491 GEAEGFLVFKQpwPGIMrtvYGNYKRFETTYfKKFP--GYYVTGDGCRRDKDGYYWITGRIDDMLNVS-GHLLSTAEVES 567
Cdd:cd05932 339 ISEDGEILVRS--PALM---MGYYKDPEATA-EAFTadGFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIEN 412
|
330 340 350 360
....*....|....*....|....*....|....*....|....*....
gi 1394780554 568 ALAEHKAISEAAVVSH--PHPVKGECLYCFVTLRDGHEFSKTLQEELKK 614
Cdd:cd05932 413 KLAEHDRVEMVCVIGSglPAPLALVVLSEEARLRADAFARAELEASLRA 461
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
481-654 |
5.66e-15 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 78.11 E-value: 5.66e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 481 ILDESGQEL-EGEAeGFLVFKQPWpgimrTVYGNYKRFE--TTYFKKfPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSG 557
Cdd:PRK10946 367 VADADGNPLpQGEV-GRLMTRGPY-----TFRGYYKSPQhnASAFDA-NGFYCSGDLVSIDPDGYITVVGREKDQINRGG 439
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 558 HLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGhefSKTLQeeLKKQVREK-IGPIATPDYIQHAPGLP 636
Cdd:PRK10946 440 EKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVKEP---LKAVQ--LRRFLREQgIAEFKLPDRVECVDSLP 514
|
170
....*....|....*...
gi 1394780554 637 KTRSGKILRRVLRQIAKN 654
Cdd:PRK10946 515 LTAVGKVDKKQLRQWLAS 532
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
117-670 |
6.04e-15 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 79.23 E-value: 6.04e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTAd 196
Cdd:PRK12316 2029 LSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLLTQ- 2107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 afyrgdklislkqiaddalqkckekgflvrncivvNHLGREELVSDGttsqsppVKRLcqdvqmpwNSDVDLWWHELmrn 276
Cdd:PRK12316 2108 -----------------------------------RHLLERLPLPAG-------VARL--------PLDRDAEWADY--- 2134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 ASKECEPEwCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYvFDYHPEDIYWCTGDIGWITGHSYLtYGPLAN 356
Cdd:PRK12316 2135 PDTAPAVQ-LAGENLAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGER-YELSPADCELQFMSFSFDGAHEQW-FHPLLN 2211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 357 GATSVLFEGvpTYPDVSRMWNIVDKYKVTKLYTSPTAIRALmksgdepVKKCSRE----SLKILGTVGEPINPEAWLWYY 432
Cdd:PRK12316 2212 GARVLIRDD--ELWDPEQLYDEMERHGVTILDFPPVYLQQL-------AEHAERDgrppAVRVYCFGGEAVPAASLRLAW 2282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 433 HVIGDERcpIVDTFWQTETgghMLTPLP-AVTAMKPGSATFPFFGVVPA-----ILDESGQEL------------EGEAE 494
Cdd:PRK12316 2283 EALRPVY--LFNGYGPTEA---VVTPLLwKCRPQDPCGAAYVPIGRALGnrrayILDADLNLLapgmagelylggEGLAR 2357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 495 GFLvfkqPWPGImrtvygNYKRFETTYFKKFPG-YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHK 573
Cdd:PRK12316 2358 GYL----NRPGL------TAERFVPDPFSASGErLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHP 2427
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 574 AISEAAVVSHPHPvKGECLYCFVTLRDGHEfskTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLrqiAK 653
Cdd:PRK12316 2428 AVREAVVVAQDGA-SGKQLVAYVVPDDAAE---DLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKAL---PK 2500
|
570
....*....|....*..
gi 1394780554 654 NDRDLGDTSTVADQSVI 670
Cdd:PRK12316 2501 PDVSQLRQAYVAPQEGL 2517
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
117-648 |
7.82e-15 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 79.05 E-value: 7.82e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTAD 196
Cdd:PRK12467 3121 LSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVPLDPEYPRERLAYMIEDSGVKLLLTQA 3200
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 AfyrgdklislkqiaddalqkckekgflvrnciVVNHLGREELVsdgttsqsppvKRLCQDvqmpwnsDVDLWwhelmrN 276
Cdd:PRK12467 3201 H--------------------------------LLEQLPAPAGD-----------TALTLD-------RLDLN------G 3224
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 ASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAAT---------------FKYVFDYHPEDIYWctgdig 341
Cdd:PRK12467 3225 YSENNPSTRVMGENLAYVIYTSGSTGKPKGVGVRHGALANHLCWIaeayeldandrvllfMSFSFDGAQERFLW------ 3298
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 342 witghsyltygPLANGATSVLFEGVPTYPDvsRMWNIVDKYKVTKLYTSPTAIRALMKSGDepVKKCSreSLKILGTVGE 421
Cdd:PRK12467 3299 -----------TLICGGCLVVRDNDLWDPE--ELWQAIHAHRISIACFPPAYLQQFAEDAG--GADCA--SLDIYVFGGE 3361
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 422 PINPEAWLWY---------YHVIGDERCPIVDTFWqtETGGHMLTPLPAVTAMKpgsatfPFFGVVPAILDESGQ----- 487
Cdd:PRK12467 3362 AVPPAAFEQVkrklkprglTNGYGPTEAVVTVTLW--KCGGDAVCEAPYAPIGR------PVAGRSIYVLDGQLNpvpvg 3433
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 488 ---EL----EGEAEGFlvFKQpwPGImrtvygNYKRFETTYFKKFPG-YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHL 559
Cdd:PRK12467 3434 vagELyiggVGLARGY--HQR--PSL------TAERFVADPFSGSGGrLYRTGDLARYRADGVIEYLGRIDHQVKIRGFR 3503
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 560 LSTAEVESALAEHKAISEAAVVSHPhPVKGECLYCFVTLrdgHEFSKTLQEELKKQVREKIgpiatPDYIQHA-----PG 634
Cdd:PRK12467 3504 IELGEIEARLLQHPSVREAVVLARD-GAGGKQLVAYVVP---ADPQGDWRETLRDHLAASL-----PDYMVPAqllvlAA 3574
|
570
....*....|....
gi 1394780554 635 LPKTRSGKILRRVL 648
Cdd:PRK12467 3575 MPLGPNGKVDRKAL 3588
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
116-648 |
2.79e-14 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 75.59 E-value: 2.79e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTA 195
Cdd:cd17656 13 KLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSGVRVVLTQ 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 dafyrgdklislkqiaddalQKCKEKgfLVRNCIVVnHLgreelvsdgttsqsppvkrlcqdvqmpwnsDVDLWWHELMR 275
Cdd:cd17656 93 --------------------RHLKSK--LSFNKSTI-LL------------------------------EDPSISQEDTS 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 NASKECEpewcdSEDELFILYTSGSTGKPKGV-------------------VHTVGGYMIFTAATFkyvfDYHPEDIY-- 334
Cdd:cd17656 120 NIDYINN-----SDDLLYIIYTSGTTGKPKGVqlehknmvnllhferektnINFSDKVLQFATCSF----DVCYQEIFst 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 335 WCTGdigwitGHSYLtygplANGATSVlfegvptypDVSRMWNIVDKYKVTKLYTsPTAIRALMKSGDE---PVKKCsre 411
Cdd:cd17656 191 LLSG------GTLYI-----IREETKR---------DVEQLFDLVKRHNIEVVFL-PVAFLKFIFSEREfinRFPTC--- 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 412 sLKILGTVGEPI---NPeawlwYYHVIGDERCPIVDTFWQTETggHMLT-----PLPAVTAM----KPGSATFPFfgvvp 479
Cdd:cd17656 247 -VKHIITAGEQLvitNE-----FKEMLHEHNVHLHNHYGPSET--HVVTtytinPEAEIPELppigKPISNTWIY----- 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 480 aILDESGQelegeaegflvfKQPWpGIMRTVY--------GNYKRFETTYFKKFPG-------YYVTGDGCRRDKDGYYW 544
Cdd:cd17656 314 -ILDQEQQ------------LQPQ-GIVGELYisgasvarGYLNRQELTAEKFFPDpfdpnerMYRTGDLARYLPDGNIE 379
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 545 ITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTlrdghefskTLQEELKKQVREKIGPiA 624
Cdd:cd17656 380 FLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFV---------MEQELNISQLREYLAK-Q 449
|
570 580
....*....|....*....|....*....
gi 1394780554 625 TPDYIQHA-----PGLPKTRSGKILRRVL 648
Cdd:cd17656 450 LPEYMIPSffvplDQLPLTPNGKVDRKAL 478
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
100-650 |
2.98e-14 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 75.79 E-value: 2.98e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYwegnDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGAlhsiVFAGFSAES 179
Cdd:PLN02330 43 DKVAFV----EAVTGKAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGG----VFSGANPTA 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 180 LCERILD----SNCSLLVTADAFYrgdklislkqiaddalQKCKEKGFLVrncIVVnhlgreelvsdGTTSQSPPVKrlc 255
Cdd:PLN02330 115 LESEIKKqaeaAGAKLIVTNDTNY----------------GKVKGLGLPV---IVL-----------GEEKIEGAVN--- 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 256 qdvqmpwnsdvdlwWHELMrNASKECEPEWCDSE----DELFILYTSGSTGKPKGVVHT--------------VGGYMIF 317
Cdd:PLN02330 162 --------------WKELL-EAADRAGDTSDNEEilqtDLCALPFSSGTTGISKGVMLThrnlvanlcsslfsVGPEMIG 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 318 TAATFKYVFDYHpedIYWCTGdigwitghsyLTYGPLANGATSVlfegVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRAL 397
Cdd:PLN02330 227 QVVTLGLIPFFH---IYGITG----------ICCATLRNKGKVV----VMSRFELRTFLNALITQEVSFAPIVPPIILNL 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 398 MKsgdEPVKK---CSRESLKILGTVGEPINPEAWLWYyhvigDERCPIV---DTFWQTETGGHMLT---PLPAVTAMKPG 468
Cdd:PLN02330 290 VK---NPIVEefdLSKLKLQAIMTAAAPLAPELLTAF-----EAKFPGVqvqEAYGLTEHSCITLThgdPEKGHGIAKKN 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 469 SATFPFFGVVPAILD-ESGQELEGEAEGFLVFKQPWpgIMRTVYGNYKRFETTYFKKfpGYYVTGDGCRRDKDGYYWITG 547
Cdd:PLN02330 362 SVGFILPNLEVKFIDpDTGRSLPKNTPGELCVRSQC--VMQGYYNNKEETDRTIDED--GWLHTGDIGYIDDDGDIFIVD 437
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 548 RIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLrdgHEFSKTLQEELKKQVREKIGPIATPD 627
Cdd:PLN02330 438 RIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGEIPAACVVI---NPKAKESEEDILNFVAANVAHYKKVR 514
|
570 580
....*....|....*....|...
gi 1394780554 628 YIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:PLN02330 515 VVQFVDSIPKSLSGKIMRRLLKE 537
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
116-648 |
3.29e-14 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 75.37 E-value: 3.29e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTa 195
Cdd:cd17652 12 TLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYMLADARPALLLT- 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 dafyrgdklislkqiaddalqkckekgflvrncivvnhlgreelvsdgttsqsppvkrlcqdvqmpwnsdvdlwwhelmr 275
Cdd:cd17652 --------------------------------------------------------------------------------
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 naskecepewcDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYvFDYHPEDIYWCTGDIGWITGHSYLTYGPLA 355
Cdd:cd17652 91 -----------TPDNLAYVIYTSGSTGRPKGVVVTHRGLANLAAAQIAA-FDVGPGSRVLQFASPSFDASVWELLMALLA 158
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 356 nGATSVLfegVPTYPDVS--RMWNIVDKYKVTKLYTSPTAIRALmKSGDEPvkkcsreSLKILGTVGEPINPE-AWLWyy 432
Cdd:cd17652 159 -GATLVL---APAEELLPgePLADLLREHRITHVTLPPAALAAL-PPDDLP-------DLRTLVVAGEACPAElVDRW-- 224
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 433 hviGDERCpIVDTFWQTETgghmlTPLPAVTAMKPGSATFPFFGVVPA----ILDESGQ--------EL----EGEAEGF 496
Cdd:cd17652 225 ---APGRR-MINAYGPTET-----TVCATMAGPLPGGGVPPIGRPVPGtrvyVLDARLRpvppgvpgELyiagAGLARGY 295
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 497 LvfkqPWPGIMRTvygnykRFETTYFKKfPG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKA 574
Cdd:cd17652 296 L----NRPGLTAE------RFVADPFGA-PGsrMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPG 364
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1394780554 575 ISEAAVVSHPHPVKGECLYCFVTLRDGhefSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd17652 365 VAEAVVVVRDDRPGDKRLVAYVVPAPG---AAPTAAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
275-650 |
3.34e-14 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 75.41 E-value: 3.34e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 275 RNASKECEPewcDSEDELFILYTSGSTGKPKGVVHTVGGymifTAATFkyvfDYHPEDIYWCTGDI-----------GWI 343
Cdd:PRK07787 117 RSWHRYPEP---DPDAPALIVYTSGTTGPPKGVVLSRRA----IAADL----DALAEAWQWTADDVlvhglplfhvhGLV 185
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 344 TGhsylTYGPLANGATSV-------------------LFEGVPTypdvsrMWN-IVDkykvtklytSPTAIRA-----LM 398
Cdd:PRK07787 186 LG----VLGPLRIGNRFVhtgrptpeayaqalseggtLYFGVPT------VWSrIAA---------DPEAARAlrgarLL 246
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 399 KSGDEPVKKCSRESLKILgTVGEPInpeawlwyyhvigdERcpivdtFWQTETggHMLTPLPAVTAMKPGSATFPFFGVV 478
Cdd:PRK07787 247 VSGSAALPVPVFDRLAAL-TGHRPV--------------ER------YGMTET--LITLSTRADGERRPGWVGLPLAGVE 303
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 479 PAILDESGQEL--EGEAEGFLVFKQPwpgimrTVYGNY-KRFETTYFKKFP-GYYVTGDGCRRDKDGYYWITGRID-DML 553
Cdd:PRK07787 304 TRLVDEDGGPVphDGETVGELQVRGP------TLFDGYlNRPDATAAAFTAdGWFRTGDVAVVDPDGMHRIVGREStDLI 377
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 554 NVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGhefskTLQEELKKQVREKIGPIATPDYIQHAP 633
Cdd:PRK07787 378 KSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADD-----VAADELIDFVAQQLSVHKRPREVRFVD 452
|
410
....*....|....*..
gi 1394780554 634 GLPKTRSGKILRRVLRQ 650
Cdd:PRK07787 453 ALPRNAMGKVLKKQLLS 469
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
289-648 |
3.73e-14 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 75.16 E-value: 3.73e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 289 EDELFILYTSGSTGKPKGVV--------HTVGGYMIFTAATFKYV-------FDYHPEDIY--WCTGdigwitghsylty 351
Cdd:cd17644 106 ENLAYVIYTSGSTGKPKGVMiehqslvnLSHGLIKEYGITSSDRVlqfasiaFDVAAEEIYvtLLSG------------- 172
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 352 gplangATSVLFEGvPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMKSGdEPVKKCSRESLKILGTVGEPINPEAW-LW 430
Cdd:cd17644 173 ------ATLVLRPE-EMRSSLEDFVQYIQQWQLTVLSLPPAYWHLLVLEL-LLSTIDLPSSLRLVIVGGEAVQPELVrQW 244
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 431 ---------YYHVIGDERCPIVDTFwqtetggHMLTPLPAVTAMKPGSATfPFFGVVPAILDESGQELEGEAEGFLVFkq 501
Cdd:cd17644 245 qknvgnfiqLINVYGPTEATIAATV-------CRLTQLTERNITSVPIGR-PIANTQVYILDENLQPVPVGVPGELHI-- 314
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 502 pwpGIMRTVYGNYKRFETTY-------FKKFPG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEH 572
Cdd:cd17644 315 ---GGVGLARGYLNRPELTAekfishpFNSSESerLYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQH 391
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1394780554 573 KAISEAAVVSHPHPVKGECLYCFVTlrdGHEFSKTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd17644 392 NDVKTAVVIVREDQPGNKRLVAYIV---PHYEESPSTVELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
287-645 |
6.48e-14 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 74.40 E-value: 6.48e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFdYHPED----------IYWCTGDigwitghsYLTygPLAN 356
Cdd:cd05914 87 DEDDVALINYTSGTTGNSKGVMLTYRNIVSNVDGVKEVVL-LGKGDkilsilplhhIYPLTFT--------LLL--PLLN 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 357 GATSVLFEGVPT-------------YPDVSRMWNIVDKYKVTKLYTSPTAI---RALMKSGDEPVKKCSRESL------- 413
Cdd:cd05914 156 GAHVVFLDKIPSakiialafaqvtpTLGVPVPLVIEKIFKMDIIPKLTLKKfkfKLAKKINNRKIRKLAFKKVheafggn 235
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 414 -KILGTVGEPINPEAwLWYYHVIGderCPIVDTFWQTETGghmltplPAVTAMKP-----GSATFPFFGVVPAILDESGQ 487
Cdd:cd05914 236 iKEFVIGGAKINPDV-EEFLRTIG---FPYTIGYGMTETA-------PIISYSPPnrirlGSAGKVIDGVEVRIDSPDPA 304
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 488 ELEGEaegfLVFKQpwPGIMRtvyGNYKRFETTYFKKFP-GYYVTGDGCRRDKDGYYWITGRIDDM-LNVSGHLLSTAEV 565
Cdd:cd05914 305 TGEGE----IIVRG--PNVMK---GYYKNPEATAEAFDKdGWFHTGDLGKIDAEGYLYIRGRKKEMiVLSSGKNIYPEEI 375
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 566 ESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIGpIATPDYIQ------HAPGLPKTR 639
Cdd:cd05914 376 EAKINNMPFVLESLVVVQEKKLVALAYIDPDFLDVKALKQRNIIDAIKWEVRDKVN-QKVPNYKKiskvkiVKEEFEKTP 454
|
....*.
gi 1394780554 640 SGKILR 645
Cdd:cd05914 455 KGKIKR 460
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
118-650 |
1.50e-13 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 73.61 E-value: 1.50e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTada 197
Cdd:cd05939 5 TFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALIF--- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 198 fyrgdklislkqiadDALQKCKEkgflvrncivvnhlgreelvsdgTTSQSPPVkrlCQDVqmpwnsdvdlwwhelmrna 277
Cdd:cd05939 82 ---------------NLLDPLLT-----------------------QSSTEPPS---QDDV------------------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 278 skecepewcDSEDELFILYTSGSTGKPKGVVHTVGGYMiFTAATFKYVFDYHPEDI-------YWCTGDIGWItGHSylt 350
Cdd:cd05939 102 ---------NFRDKLFYIYTSGTTGLPKAAVIVHSRYY-RIAAGAYYAFGMRPEDVvydclplYHSAGGIMGV-GQA--- 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 351 ygpLANGATSVL---FEGvptypdvSRMWNIVDKYKVTKLYTSPTAIRALMKSgdEPVKKCSRESLKILgtVGEPINPEA 427
Cdd:cd05939 168 ---LLHGSTVVIrkkFSA-------SNFWDDCVKYNCTIVQYIGEICRYLLAQ--PPSEEEQKHNVRLA--VGNGLRPQI 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 428 WLWYyhvIGDERCPIVDTFW-QTETGGHMLTPLPAVTAMKPGSATFPFFGVVPAI-LDESGQELEGEAEGFLVFKQPW-P 504
Cdd:cd05939 234 WEQF---VRRFGIPQIGEFYgATEGNSSLVNIDNHVGACGFNSRILPSVYPIRLIkVDEDTGELIRDSDGLCIPCQPGeP 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 505 GIM--RTVYGN-YKRFE-------------TTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESA 568
Cdd:cd05939 311 GLLvgKIIQNDpLRRFDgyvnegatnkkiaRDVFKKGDSAFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGI 390
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 569 LAEHKAISEAAV--VSHPHpVKGEC----LYCFVTLRDGHEFSKTLQEELKkqvrekigPIATPDYIQHAPGLPKTRSGK 642
Cdd:cd05939 391 LSNVLGLEDVVVygVEVPG-VEGRAgmaaIVDPERKVDLDRFSAVLAKSLP--------PYARPQFIRLLPEVDKTGTFK 461
|
....*...
gi 1394780554 643 ILRRVLRQ 650
Cdd:cd05939 462 LQKTDLQK 469
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
287-652 |
4.03e-13 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 72.19 E-value: 4.03e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVV--HTvggyMIFTAAT-FKYVFDYHPE-----------DIywCTGDIgwitghsyltYG 352
Cdd:cd05918 104 SPSDAAYVIFTSGSTGKPKGVVieHR----ALSTSALaHGRALGLTSEsrvlqfasytfDV--SILEI----------FT 167
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 353 PLANGATSVlfegVPtyPDVSRMWNIVD---KYKVTKLYTSPTAIRaLMKSGDEPvkkcsreSLKILGTVGEPINPEawl 429
Cdd:cd05918 168 TLAAGGCLC----IP--SEEDRLNDLAGfinRLRVTWAFLTPSVAR-LLDPEDVP-------SLRTLVLGGEALTQS--- 230
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 430 wyyhvigdercpIVDTFWQ----------TETGGHMLTPLPaVTAMKPGSATFPFfGVVPAILDESGQE----------- 488
Cdd:cd05918 231 ------------DVDTWADrvrlinaygpAECTIAATVSPV-VPSTDPRNIGRPL-GATCWVVDPDNHDrlvpigavgel 296
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 489 -LEGE--AEGFL--------VFKQPWPGIMRTVYGNYKRFettyfkkfpgyYVTGDGCRRDKDG--YYwiTGRIDDMLNV 555
Cdd:cd05918 297 lIEGPilARGYLndpektaaAFIEDPAWLKQEGSGRGRRL-----------YRTGDLVRYNPDGslEY--VGRKDTQVKI 363
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 556 SGHLLSTAEVESALAEHKAISE---AAVVSHPHPVKGECLYCFVTLRDGHEfSKTLQEELKKQVREKIGPIAT------- 625
Cdd:cd05918 364 RGQRVELGEIEHHLRQSLPGAKevvVEVVKPKDGSSSPQLVAFVVLDGSSS-GSGDGDSLFLEPSDEFRALVAelrsklr 442
|
410 420 430
....*....|....*....|....*....|....*...
gi 1394780554 626 ---PDY--------IQHapgLPKTRSGKILRRVLRQIA 652
Cdd:cd05918 443 qrlPSYmvpsvflpLSH---LPLTASGKIDRRALRELA 477
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
118-649 |
5.75e-13 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 71.75 E-value: 5.75e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIympmiielvvamlacarigalhsivfAGFSAESLCERILDSNCSLLVTADA 197
Cdd:PLN02860 34 TGHEFVDGVLSLAAGLLRLGLRNGDVVAI--------------------------AALNSDLYLEWLLAVACAGGIVAPL 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 198 FYRGdkliSLKQiADDALQkckekgfLVRNCIvvnhlgreeLVSDGTTSQsppvkrLCQDVQmpwNSDVDLW-WHELMRN 276
Cdd:PLN02860 88 NYRW----SFEE-AKSAML-------LVRPVM---------LVTDETCSS------WYEELQ---NDRLPSLmWQVFLES 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 -----------------------ASKECEPEWCdSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVfDYHPEDI 333
Cdd:PLN02860 138 psssvfiflnsflttemlkqralGTTELDYAWA-PDDAVLICFTSGTTGRPKGVTISHSALIVQSLAKIAIV-GYGEDDV 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 334 YWCTGDIGWITGHSyltyGPLAN---GATSVLfegVPTYpDVSRMWNIVDKYKVTKLYTSPTAIRALMKSG-DEPVKKCS 409
Cdd:PLN02860 216 YLHTAPLCHIGGLS----SALAMlmvGACHVL---LPKF-DAKAALQAIKQHNVTSMITVPAMMADLISLTrKSMTWKVF 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 410 RESLKIL---GTVGEPINPEAWLWYyhvigdERCPIVDTFWQTETGGHMltplpavTAMKPGSATFPFFGVVPAILDESG 486
Cdd:PLN02860 288 PSVRKILnggGSLSSRLLPDAKKLF------PNAKLFSAYGMTEACSSL-------TFMTLHDPTLESPKQTLQTVNQTK 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 487 QELEGEAEGFLVFKQP---------------------WPGIMRTVYGNYKrfETTYFKKFPGYYVTGDGCRRDKDGYYWI 545
Cdd:PLN02860 355 SSSVHQPQGVCVGKPAphvelkigldessrvgriltrGPHVMLGYWGQNS--ETASVLSNDGWLDTGDIGWIDKAGNLWL 432
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 546 TGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFS----------KTL-QEELKK 614
Cdd:PLN02860 433 IGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVPDSRLTEMVVACVRLRDGWIWSdnekenakknLTLsSETLRH 512
|
570 580 590
....*....|....*....|....*....|....*..
gi 1394780554 615 QVREK-IGPIATPD-YIQHAPGLPKTRSGKILRRVLR 649
Cdd:PLN02860 513 HCREKnLSRFKIPKlFVQWRKPFPLTTTGKIRRDEVR 549
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
116-648 |
5.96e-13 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 72.89 E-value: 5.96e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTA 195
Cdd:PRK05691 2213 TLSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLDPEYPLERLHYMIEDSGIGLLLSD 2292
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 DAFYRgdkliSLKQIADDALQKCKEkgflvrncivvnhlgrEELVSDGTTSQSPPvkrlcQDVQMPwnsdvdlwwhelmr 275
Cdd:PRK05691 2293 RALFE-----ALGELPAGVARWCLE----------------DDAAALAAYSDAPL-----PFLSLP-------------- 2332
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 naskecepewcdsEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKyVFDYHPEDIYWCTGDIGWITGHSYLtYGPLA 355
Cdd:PRK05691 2333 -------------QHQAYLIYTSGSTGKPKGVVVSHGEIAMHCQAVIE-RFGMRADDCELHFYSINFDAASERL-LVPLL 2397
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 356 NGATSVL-FEGvptYPDVSRMWNIVDKYKVTKL-----YTSPTAiRALMKSGDE-PVKKCsreslkILGtvGEPINPEAW 428
Cdd:PRK05691 2398 CGARVVLrAQG---QWGAEEICQLIREQQVSILgftpsYGSQLA-QWLAGQGEQlPVRMC------ITG--GEALTGEHL 2465
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 429 LWYYHVIGDERcpIVDTFWQTETgghMLTPL--PAVTAMKPGSATFPFFGVVPA----ILDE---------SGqEL---- 489
Cdd:PRK05691 2466 QRIRQAFAPQL--FFNAYGPTET---VVMPLacLAPEQLEEGAASVPIGRVVGArvayILDAdlalvpqgaTG-ELyvgg 2539
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 490 EGEAEGFlvfkQPWPGImrtvygNYKRFETTYFKKFPG-YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESA 568
Cdd:PRK05691 2540 AGLAQGY----HDRPGL------TAERFVADPFAADGGrLYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESR 2609
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 569 LAEHKAISEAAVVSHPHPvKGECLYCFV---TLRDGHEFSKTLQEELKKQVREKIgpiatPDYIQHA-----PGLPKTRS 640
Cdd:PRK05691 2610 LLEHPAVREAVVLALDTP-SGKQLAGYLvsaVAGQDDEAQAALREALKAHLKQQL-----PDYMVPAhlillDSLPLTAN 2683
|
....*...
gi 1394780554 641 GKILRRVL 648
Cdd:PRK05691 2684 GKLDRRAL 2691
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
117-649 |
8.45e-13 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 71.16 E-value: 8.45e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACarigalhsiVFAGFSAeslcerildsncsLLVTAD 196
Cdd:cd05906 40 QSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWAC---------VLAGFVP-------------APLTVP 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 AFYrgdklislkQIADDALQKCKEKGFLVRNCIVvnhLGREELVSD----GTTSQSPPVKRLCQDvqmpwnsdvdlwwhE 272
Cdd:cd05906 98 PTY---------DEPNARLRKLRHIWQLLGSPVV---LTDAELVAEfaglETLSGLPGIRVLSIE--------------E 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 273 LMRNASkecEPEW--CDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKyVFDYHPEDIY--W------------- 335
Cdd:cd05906 152 LLDTAA---DHDLpqSRPDDLALLMLTSGSTGFPKAVPLTHRNILARSAGKIQ-HNGLTPQDVFlnWvpldhvgglvelh 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 336 -------C------TGDI--------GWITGHSY-LTYGPlaNGATSVLFEGVPTYPDVSrmWNIvdkykvtklytspTA 393
Cdd:cd05906 228 lravylgCqqvhvpTEEIladplrwlDLIDRYRVtITWAP--NFAFALLNDLLEEIEDGT--WDL-------------SS 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 394 IRALMKSGDEPVKKCSRESLKILGTVGEPinPEAwlwyyhvigdercpIVDTFWQTETGGHMLTPLPAVTAMKPGSATF- 472
Cdd:cd05906 291 LRYLVNAGEAVVAKTIRRLLRLLEPYGLP--PDA--------------IRPAFGMTETCSGVIYSRSFPTYDHSQALEFv 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 473 ----PFFGVVPAILDESGQELEGEAEGFLVFKqpwpGIMRTVyGNYKRFETT--YFKKfPGYYVTGD-GCRRDkdGYYWI 545
Cdd:cd05906 355 slgrPIPGVSMRIVDDEGQLLPEGEVGRLQVR----GPVVTK-GYYNNPEANaeAFTE-DGWFRTGDlGFLDN--GNLTI 426
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 546 TGRIDDMLNVSGHLLSTAEVESAL--AEHKAISEAAVVSHPHPVKGECLYCFV-----TLRDghEFSKTLQeELKKQVRE 618
Cdd:cd05906 427 TGRTKDTIIVNGVNYYSHEIEAAVeeVPGVEPSFTAAFAVRDPGAETEELAIFfvpeyDLQD--ALSETLR-AIRSVVSR 503
|
570 580 590
....*....|....*....|....*....|....*
gi 1394780554 619 KIGpiATPDYI----QHApgLPKTRSGKILRRVLR 649
Cdd:cd05906 504 EVG--VSPAYLiplpKEE--IPKTSLGKIQRSKLK 534
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
96-650 |
4.31e-12 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 69.19 E-value: 4.31e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 96 KKLGDKIAFYWEGNDPGISMSITYRQLLQDVCQFASVLQEQGiKKGDRISIYMPMIIELVVAMLACARIGALHSIVFA-- 173
Cdd:cd05931 4 AARPDRPAYTFLDDEGGREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLPPpt 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 174 -GFSAESLCERILDSNCSLLVTADAFYRGDKLISLKQIADDALQkckekgflvrnCIVVnhlgreELVSDGTTSQSPPVK 252
Cdd:cd05931 83 pGRHAERLAAILADAGPRVVLTTAAALAAVRAFAASRPAAGTPR-----------LLVV------DLLPDTSAADWPPPS 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 253 rlcqdvqmpwnsdvdlwwhelmrnaskecepewCDSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKyVFDYHPED 332
Cdd:cd05931 146 ---------------------------------PDPDDIAYLQYTSGSTGTPKGVVVTHRNLLANVRQIRR-AYGLDPGD 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 333 IY--W--CTGDIGWITGhsYLTygPLANGATSVLfegvptypdvsrmwnivdkykvtklyTSPTA--------IRALMKS 400
Cdd:cd05931 192 VVvsWlpLYHDMGLIGG--LLT--PLYSGGPSVL--------------------------MSPAAflrrplrwLRLISRY 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 401 G-----------DEPVKKCSRE--------SLKILGTVGEPINP---------------------------EAWLwYYHV 434
Cdd:cd05931 242 RatisaapnfayDLCVRRVRDEdlegldlsSWRVALNGAEPVRPatlrrfaeafapfgfrpeafrpsyglaEATL-FVSG 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 435 IGDERCPIVDTFWQTETGGHMLtplpAVTAMKPGSATFPFFG-VVP----AILD-ESGQELEGEAEGFLvfkqpW---PG 505
Cdd:cd05931 321 GPPGTGPVVLRVDRDALAGRAV----AVAADDPAARELVSCGrPLPdqevRIVDpETGRELPDGEVGEI-----WvrgPS 391
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 506 IMRTVYGNYKRFETTYFKKFP----GYYVTGD-GCRRDkdGYYWITGRIDDMLNVSGHLLSTAEVESALAEhkaiseaav 580
Cdd:cd05931 392 VASGYWGRPEATAETFGALAAtdegGWLRTGDlGFLHD--GELYITGRLKDLIIVRGRNHYPQDIEATAEE--------- 460
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 581 vSHPHPVKGeCLYCFVTLRDGHE-----------FSKTLQEELKKQVREKIGP---IATPDYIQHAPG-LPKTRSGKILR 645
Cdd:cd05931 461 -AHPALRPG-CVAAFSVPDDGEErlvvvaevergADPADLAAIAAAIRAAVARehgVAPADVVLVRPGsIPRTSSGKIQR 538
|
....*
gi 1394780554 646 RVLRQ 650
Cdd:cd05931 539 RACRA 543
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
290-641 |
4.43e-12 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 67.71 E-value: 4.43e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 290 DELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKyvfdyhpediywctgdIGWITGHS-YLTYGPLANGATsvLFEGVPT 368
Cdd:cd17636 1 DPVLAIYTAAFSGRPNGALLSHQALLAQALVLAV----------------LQAIDEGTvFLNSGPLFHIGT--LMFTLAT 62
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 369 Y-----------PDVSRMWNIVDKYKVTKLYTSPTAIRALMK-SGDEPVKKCSRESLKILGTVGEPINPEAWLWYYHVIG 436
Cdd:cd17636 63 FhaggtnvfvrrVDAEEVLELIEAERCTHAFLLPPTIDQIVElNADGLYDLSSLRSSPAAPEWNDMATVDTSPWGRKPGG 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 437 dercpivdtFWQTETGGhmLTPLPAVTAMKPGSATFPFFGVVPAILDESGQEL-EGEAeGFLVFKQPwpgimrTVY-GNY 514
Cdd:cd17636 143 ---------YGQTEVMG--LATFAALGGGAIGGAGRPSPLVQVRILDEDGREVpDGEV-GEIVARGP------TVMaGYW 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 515 KRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYC 594
Cdd:cd17636 205 NRPEVNARRTRGGWHHTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKA 284
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 1394780554 595 FVTLRDGHEFSktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSG 641
Cdd:cd17636 285 IVVLKPGASVT---EAELIEHCRARIASYKKPKSVEFADALPRTAGG 328
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
287-646 |
2.87e-11 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 66.27 E-value: 2.87e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFK-----------------YVFDYHPEDIYwctgdIGWITGHSYL 349
Cdd:cd17648 92 NSTDLAYAIYTSGTTGKPKGVLVEHGSVVNLRTSLSEryfgrdngdeavlffsnYVFDFFVEQMT-----LALLNGQKLV 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 350 tygplangatsVLFEGVPTYPDvsRMWNIVDKYKVTKLYTSPTAIRALMKSgdepvkkcSRESLKILGTVGEPINPEAwl 429
Cdd:cd17648 167 -----------VPPDEMRFDPD--RFYAYINREKVTYLSGTPSVLQQYDLA--------RLPHLKRVDAAGEEFTAPV-- 223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 430 wYYHVIGDERCPIVDTFWQTETgghmltplpAVTAMK---PGSATF------PFFGVVPAILDESGQ--------EL--- 489
Cdd:cd17648 224 -FEKLRSRFAGLIINAYGPTET---------TVTNHKrffPGDQRFdkslgrPVRNTKCYVLNDAMKrvpvgavgELylg 293
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 490 -EGEAEGFLvfKQPWPGIMRTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESA 568
Cdd:cd17648 294 gDGVARGYL--NRPELTAERFLPNPFQTEQERARGRNARLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAA 371
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 569 LAEHKAISEAAVVSHPHPVKGEC-----LYCFVTLRDGHeFSKTlqeELKKQVREKIGPIATPDYIQHAPGLPKTRSGKI 643
Cdd:cd17648 372 LASYPGVRECAVVAKEDASQAQSriqkyLVGYYLPEPGH-VPES---DLLSFLRAKLPRYMVPARLVRLEGIPVTINGKL 447
|
....
gi 1394780554 644 -LRR 646
Cdd:cd17648 448 dVRA 451
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
117-355 |
1.06e-10 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 64.75 E-value: 1.06e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTad 196
Cdd:PLN02387 107 ITYGQVFERVCNFASGLVALGHNKEERVAIFADTRAEWLIALQGCFRQNITVVTIYASLGEEALCHSLNETEVTTVIC-- 184
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 afyrGDKliSLKQIADdalqkCKEKGFLVRNCIVVNHLGRE-----ELVSDGTTSQSPPVKRLCQ----DVQMPWNSDVD 267
Cdd:PLN02387 185 ----DSK--QLKKLID-----ISSQLETVKRVIYMDDEGVDsdsslSGSSNWTVSSFSEVEKLGKenpvDPDLPSPNDIA 253
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 268 LwwhelmrnaskecepewcdsedelfILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPEDIywctgdigwitghs 347
Cdd:PLN02387 254 V-------------------------IMYTSGSTGLPKGVMMTHGNIVATVAGVMTVVPKLGKNDV-------------- 294
|
....*...
gi 1394780554 348 YLTYGPLA 355
Cdd:PLN02387 295 YLAYLPLA 302
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
116-656 |
1.51e-10 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 64.26 E-value: 1.51e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAeslcerildsncsllVTA 195
Cdd:PRK05857 41 ALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKLGAIAVMADGNLPI---------------AAI 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 DAFyrgdklislKQIADDAlqkckekgflvrnCIVVNHLGREElvsdgtTSQSPPVKRLCQDVQMPWNSDVDLWWHELMR 275
Cdd:PRK05857 106 ERF---------CQITDPA-------------AALVAPGSKMA------SSAVPEALHSIPVIAVDIAAVTRESEHSLDA 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 NASKeCEPEWcDSEDELFILYTSGSTGKPKGVvhtvggymIFTAATFKYVFD-YHPEDIYWctgdIGWITGHSylTYGPL 354
Cdd:PRK05857 158 ASLA-GNADQ-GSEDPLAMIFTSGTTGEPKAV--------LLANRTFFAVPDiLQKEGLNW----VTWVVGET--TYSPL 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 355 AngATSV---------LFEG---VPTYPDVSRMWNIVDKYKVTKLYTSPTAIRAL---MKSGDEPVKkcsreSLKILGTV 419
Cdd:PRK05857 222 P--ATHIgglwwiltcLMHGglcVTGGENTTSLLEILTTNAVATTCLVPTLLSKLvseLKSANATVP-----SLRLVGYG 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 420 GEPInpeawlwyyhVIGDER------CPIVDTFWQTETGGHMLTpLP----AVTAMKPGSATFPFFGVVPAILDESG--- 486
Cdd:PRK05857 295 GSRA----------IAADVRfieatgVRTAQVYGLSETGCTALC-LPtddgSIVKIEAGAVGRPYPGVDVYLAATDGigp 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 487 -QELEGEAEGFLVFKQPWPGIMRTVYGNYKRFETTYFKkfpGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEV 565
Cdd:PRK05857 364 tAPGAGPSASFGTLWIKSPANMLGYWNNPERTAEVLID---GWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEV 440
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 566 ESALAEHKAISEAAVVSHPHPVKGECLYCFVTlrDGHEFSKTLQEELKKQV----REKIGPIATPDYIQHAPGLPKTRSG 641
Cdd:PRK05857 441 DRIAEGVSGVREAACYEIPDEEFGALVGLAVV--ASAELDESAARALKHTIaarfRRESEPMARPSTIVIVTDIPRTQSG 518
|
570
....*....|....*
gi 1394780554 642 KILRRVLRQIAKNDR 656
Cdd:PRK05857 519 KVMRASLAAAATADK 533
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
117-363 |
3.94e-10 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 63.07 E-value: 3.94e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCERILDSNCSLLVTad 196
Cdd:PTZ00216 122 ITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALRETECKAIVC-- 199
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 197 afyRGDKLISLkqiaddaLQKCKEKGFLvrNCIVVnHLGreELVSDGTTSQsppvkrlCQDVqmPWnSDVdlwwheLMRN 276
Cdd:PTZ00216 200 ---NGKNVPNL-------LRLMKSGGMP--NTTII-YLD--SLPASVDTEG-------CRLV--AW-TDV------VAKG 248
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 277 ASKECE-----PEWCDseDELFILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDY--HPEDiywctgdigwitGHSYL 349
Cdd:PTZ00216 249 HSAGSHhplniPENND--DLALIMYTSGTTGDPKGVMHTHGSLTAGILALEDRLNDLigPPEE------------DETYC 314
|
250
....*....|....*...
gi 1394780554 350 TYGPLAN----GATSVLF 363
Cdd:PTZ00216 315 SYLPLAHimefGVTNIFL 332
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
287-650 |
4.64e-10 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 62.45 E-value: 4.64e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVVHTVGGYMIfTAATFKYVFDYHPED-IYWCT----GdigwiTGHSYLTYGPLANGATSV 361
Cdd:cd05937 85 DPDDPAILIYTSGTTGLPKAAAISWRRTLV-TSNLLSHDLNLKNGDrTYTCMplyhG-----TAAFLGACNCLMSGGTLA 158
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 362 L---FEgvptypdVSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLkilgTVGEPINPEAWLWYyhvigDE 438
Cdd:cd05937 159 LsrkFS-------ASQFWKDVRDSGATIIQYVGELCRYLLSTPPSPYDRDHKVRV----AWGNGLRPDIWERF-----RE 222
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 439 R--CPIVDTFWQ-TETGGHMLT----PLPAVTAMKPGSATFPFFG--VVPAILD-ESGQELEGEAEGFL----------- 497
Cdd:cd05937 223 RfnVPEIGEFYAaTEGVFALTNhnvgDFGAGAIGHHGLIRRWKFEnqVVLVKMDpETDDPIRDPKTGFCvrapvgepgem 302
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 498 ---VFKQP---WPGIMRTVYGNYKRFETTYFKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAE 571
Cdd:cd05937 303 lgrVPFKNreaFQGYLHNEDATESKLVRDVFRKGDIYFRTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGA 382
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 572 HKAISEAAV--VSHP-HPVKGECLYCFVTLRDGHEFSKTLQeELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd05937 383 HPDIAEANVygVKVPgHDGRAGCAAITLEESSAVPTEFTKS-LLASLARKNLPSYAVPLFLRLTEEVATTDNHKQQKGVL 461
|
..
gi 1394780554 649 RQ 650
Cdd:cd05937 462 RD 463
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
527-648 |
5.34e-10 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 62.32 E-value: 5.34e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 527 GYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGhefSK 606
Cdd:PRK13383 396 GMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHPG---SG 472
|
90 100 110 120
....*....|....*....|....*....|....*....|..
gi 1394780554 607 TLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:PRK13383 473 VDAAQLRDYLKDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
287-583 |
7.56e-10 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 61.61 E-value: 7.56e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTGDIgWitgHSY---LTYGPLANGAtSVLF 363
Cdd:cd17640 86 DSDDLATIIYTSGTTGNPKGVMLTHAN-LLHQIRSLSDIVPPQPGDRFLSILPI-W---HSYersAEYFIFACGC-SQAY 159
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 364 EGVPTYPDvsrmwnivD--KYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTV---GE---PIN-----PEAWLW 430
Cdd:cd17640 160 TSIRTLKD--------DlkRVKPHYIVSVPRLWESLYSGIQKQVSKSSPIKQFLFLFFlsgGIfkfGISgggalPPHVDT 231
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 431 YYHVIGderCPIVDTFWQTETGghmltplPAVTAMKP-----GSATFPFFGVVPAILDESGQE-LEGEAEGFLVFKQpwP 504
Cdd:cd17640 232 FFEAIG---IEVLNGYGLTETS-------PVVSARRLkcnvrGSVGRPLPGTEIKIVDPEGNVvLPPGEKGIVWVRG--P 299
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 505 GIMRtvyGNYKRFETTyfKKF---PGYYVTGDGCRRDKDGYYWITGRIDDMLNVS-GHLLSTAEVESALAEHKAISEAAV 580
Cdd:cd17640 300 QVMK---GYYKNPEAT--SKVldsDGWFNTGDLGWLTCGGELVLTGRAKDTIVLSnGENVEPQPIEEALMRSPFIEQIMV 374
|
...
gi 1394780554 581 VSH 583
Cdd:cd17640 375 VGQ 377
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
526-649 |
9.03e-10 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 60.83 E-value: 9.03e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 526 PGYYVTGDGCRRDkDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVtLRDGHEfS 605
Cdd:PRK07824 233 PGWFRTDDLGALD-DGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAV-VGDGGP-A 309
|
90 100 110 120
....*....|....*....|....*....|....*....|....
gi 1394780554 606 KTLqEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLR 649
Cdd:PRK07824 310 PTL-EALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALV 352
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
287-648 |
9.77e-10 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 61.42 E-value: 9.77e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVvhtvggyMIFTAATFKYVFDYHPediywctgdigwitghsYLTYGPLANGA--TSVLFE 364
Cdd:cd17645 102 NPDDLAYVIYTSGSTGLPKGV-------MIEHHNLVNLCEWHRP-----------------YFGVTPADKSLvyASFSFD 157
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 365 G----VPTYPDVSRMWNIVD---KYKVTKL----YTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEPINPEAWLWY-- 431
Cdd:cd17645 158 AsaweIFPHLTAGAALHVVPserRLDLDALndyfNQEGITISFLPTGAAEQFMQLDNQSLRVLLTGGDKLKKIERKGYkl 237
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 432 YHVIGDERCPIVDTFWQTETgghmltPLPAVTAMKPGSATFPFfgvvpaILDESGQ-ELEGEAEGFLVFKQpwpGIMRtv 510
Cdd:cd17645 238 VNNYGPTENTVVATSFEIDK------PYANIPIGKPIDNTRVY------ILDEALQlQPIGVAGELCIAGE---GLAR-- 300
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 511 yGNYKRFETTYfKKF------PG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVS 582
Cdd:cd17645 301 -GYLNRPELTA-EKFivhpfvPGerMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLA 378
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1394780554 583 HPHPVKGECLYCFVTLRDGHEfsktlQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:cd17645 379 KEDADGRKYLVAYVTAPEEIP-----HEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
116-643 |
1.57e-09 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 60.56 E-value: 1.57e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGAlhsiVFAGFSAESLCERildsncsllvta 195
Cdd:cd17654 16 TVSYADLAEKISNLSNFLRKKFQTEERAIGLRCDRGTESPVAILAILFLGA----AYAPIDPASPEQR------------ 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 196 dafyrgdkliSLKQIADDALQkckekgflvrnCIVVNhlgreelvsdgttsqsppvKRLCQDVQMPWNSDVdlwwHELMR 275
Cdd:cd17654 80 ----------SLTVMKKCHVS-----------YLLQN-------------------KELDNAPLSFTPEHR----HFNIR 115
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 276 NASKECepewcdsedelFILYTSGSTGKPKGVvhTVGGYMIFTAAT-FKYVFDYHPEDIYWCTgdigwitghSYLTYGP- 353
Cdd:cd17654 116 TDECLA-----------YVIHTSGTTGTPKIV--AVPHKCILPNIQhFRSLFNITSEDILFLT---------SPLTFDPs 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 354 -------LANGATSVLfegVPTYPDV--SRMWNIVDK-YKVTKLYTSPTAIRALMKSGDEPVKKCSRESLKILGTVGEPI 423
Cdd:cd17654 174 vveiflsLSSGATLLI---VPTSVKVlpSKLADILFKrHRITVLQATPTLFRRFGSQSIKSTVLSATSSLRVLALGGEPF 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 424 nP-----EAWLWYYHvigdeRCPIVDTFWQTETGGHMLTplpavTAMKPGSAT----FPFFGVVPAILDESGQELEGEAE 494
Cdd:cd17654 251 -PslvilSSWRGKGN-----RTRIFNIYGITEVSCWALA-----YKVPEEDSPvqlgSPLLGTVIEVRDQNGSEGTGQVF 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 495 GFLVFKQpwpGIMRTVYGnykrfettyfKKFPGYYVTGDGCRRdKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKA 574
Cdd:cd17654 320 LGGLNRV---CILDDEVT----------VPKGTMRATGDFVTV-KDGELFFLGRKDSQIKRRGKRINLDLIQQVIESCLG 385
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1394780554 575 ISEAAVVSHphpvKGECLYCFVTlrdGHEFSKTLQEELKKQVREkigPIATPDYIQHAPGLPKTRSGKI 643
Cdd:cd17654 386 VESCAVTLS----DQQRLIAFIV---GESSSSRIHKELQLTLLS---SHAIPDTFVQIDKLPLTSHGKV 444
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
110-586 |
4.17e-09 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 59.74 E-value: 4.17e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 110 DPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALhSIvfaGFSAESLCERI---LD 186
Cdd:cd17641 5 DFGIWQEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGAL-SL---GIYQDSMAEEVaylLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 187 SNCSLLVTADAFYRGDKLIslkQIADDAlqkckekgflvrncivvnHLGREELVSDgttsqsPPVKRLCQDVQMPWNSDV 266
Cdd:cd17641 81 YTGARVVIAEDEEQVDKLL---EIADRI------------------PSVRYVIYCD------PRGMRKYDDPRLISFEDV 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 267 dlwwHELMRNASKEcEPEWCDSE------DELFIL-YTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIYWCTGD 339
Cdd:cd17641 134 ----VALGRALDRR-DPGLYEREvaagkgEDVAVLcTTSGTTGKPKLAMLSHGN-FLGHCAAYLAADPLGPGDEYVSVLP 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 340 IGWITGHSYLTYGPLANGATSVLFEGVPTY--------PDV----SRMW-NIVDKYKVTKLYTSP---TAIRALMKSG-- 401
Cdd:cd17641 208 LPWIGEQMYSVGQALVCGFIVNFPEEPETMmedlreigPTFvllpPRVWeGIAADVRARMMDATPfkrFMFELGMKLGlr 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 402 -------DEPVKKCSRES--------------------LKILGTVGEPINPEAWLwYYHVIGderCPIVDTFWQTETGGh 454
Cdd:cd17641 288 aldrgkrGRPVSLWLRLAswladallfrplrdrlgfsrLRSAATGGAALGPDTFR-FFHAIG---VPLKQLYGQTELAG- 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 455 mltplpAVTAMKPGSATFPFFGVVPAildesGQELEGEAEGFLVFKQPwpgimRTVYGNYKRFETTYFKKFP-GYYVTGD 533
Cdd:cd17641 363 ------AYTVHRDGDVDPDTVGVPFP-----GTEVRIDEVGEILVRSP-----GVFVGYYKNPEATAEDFDEdGWLHTGD 426
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 1394780554 534 GCRRDKDGYYWITGRIDDMLNVS-GHLLSTAEVESALAEHKAISEAAVVSHPHP 586
Cdd:cd17641 427 AGYFKENGHLVVIDRAKDVGTTSdGTRFSPQFIENKLKFSPYIAEAVVLGAGRP 480
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
529-648 |
6.95e-09 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 59.41 E-value: 6.95e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 529 YVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPvKGECLYCFVTLRDGHEFSKTL 608
Cdd:PRK05691 4104 YRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAAVAVQEGV-NGKHLVGYLVPHQTVLAQGAL 4182
|
90 100 110 120
....*....|....*....|....*....|....*....|
gi 1394780554 609 QEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:PRK05691 4183 LERIKQRLRAELPDYMVPLHWLWLDRLPLNANGKLDRKAL 4222
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
116-310 |
1.02e-08 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 58.24 E-value: 1.02e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQ-EQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLCErILDSNCSLLVT 194
Cdd:cd17632 67 TITYAELWERVGAVAAAHDpEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAP-ILAETEPRLLA 145
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 195 ADAfyrgDKLislkqiaDDALQKCKEkGFLVRNCIVVNHlgREELvsDGTTSQSPPVKRLCQDVQMPWNSDVDLWWHELM 274
Cdd:cd17632 146 VSA----EHL-------DLAVEAVLE-GGTPPRLVVFDH--RPEV--DAHRAALESARERLAAVGIPVTTLTLIAVRGRD 209
|
170 180 190
....*....|....*....|....*....|....*..
gi 1394780554 275 RNASKECEPEwcDSEDEL-FILYTSGSTGKPKGVVHT 310
Cdd:cd17632 210 LPPAPLFRPE--PDDDPLaLLIYTSGSTGTPKGAMYT 244
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
268-654 |
1.24e-08 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 58.18 E-value: 1.24e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 268 LW--WHELM-RNASKECEPEwcdseDELFILYTSGSTGKPKGVVHTVGGYMIfTAATFKYVFDYHPEDIYWCTGDIGwit 344
Cdd:PRK08043 346 LWifAHLLMpRLAQVKQQPE-----DAALILFTSGSEGHPKGVVHSHKSLLA-NVEQIKTIADFTPNDRFMSALPLF--- 416
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 345 gHSY-LTYG---PLANGATSVLFEGVPTYPDVSRMwnIVDKyKVTKLYTSPTairalmksgdepvkkcsreslkILGTVG 420
Cdd:PRK08043 417 -HSFgLTVGlftPLLTGAEVFLYPSPLHYRIVPEL--VYDR-NCTVLFGTST----------------------FLGNYA 470
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 421 EPINP-EAWLWYYHVIGDERC-PIVDTFWQTETG-----GHMLT---PLPAVT---AMKPGSATFPFFGV------VPAI 481
Cdd:PRK08043 471 RFANPyDFARLRYVVAGAEKLqESTKQLWQDKFGlrileGYGVTecaPVVSINvpmAAKPGTVGRILPGMdarllsVPGI 550
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 482 LDESGQELEGeaegflvfkqpwPGIMRtvygNYKRFETTYFKKFP-----------GYYVTGDGCRRDKDGYYWITGRID 550
Cdd:PRK08043 551 EQGGRLQLKG------------PNIMN----GYLRVEKPGVLEVPtaenargemerGWYDTGDIVRFDEQGFVQIQGRAK 614
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 551 DMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTlrDghefSKTLQEELKKQVREKIGP-IATPDYI 629
Cdd:PRK08043 615 RFAKIAGEMVSLEMVEQLALGVSPDKQHATAIKSDASKGEALVLFTT--D----SELTREKLQQYAREHGVPeLAVPRDI 688
|
410 420
....*....|....*....|....*
gi 1394780554 630 QHAPGLPKTRSGKILRRVLRQIAKN 654
Cdd:PRK08043 689 RYLKQLPLLGSGKPDFVTLKSMVDE 713
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
527-650 |
1.64e-08 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 57.87 E-value: 1.64e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 527 GYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGHEFSK 606
Cdd:PRK05620 430 GWLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTR 509
|
90 100 110 120
....*....|....*....|....*....|....*....|....
gi 1394780554 607 TLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQ 650
Cdd:PRK05620 510 ETAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDLRQ 553
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
100-648 |
1.96e-08 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 58.26 E-value: 1.96e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAES 179
Cdd:PRK05691 1146 ERIALVWDGG------SLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLDPDYPAER 1219
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 180 LCERILDSNCSLLVTADAFYrgDKLISLKQIADDALqkckekgflvrncivvnhlgrEELVSDGTTSQSPpvkrlcqdvq 259
Cdd:PRK05691 1220 LAYMLADSGVELLLTQSHLL--ERLPQAEGVSAIAL---------------------DSLHLDSWPSQAP---------- 1266
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 260 mpwnsDVDLWWHELMrnaskecepewcdsedelFILYTSGSTGKPKGVVHTVGGYM---IFTAATfkYVFDyhPEDIYWC 336
Cdd:PRK05691 1267 -----GLHLHGDNLA------------------YVIYTSGSTGQPKGVGNTHAALAerlQWMQAT--YALD--DSDVLMQ 1319
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 337 TGDIGWITGhSYLTYGPLANGATSVLfEGVPTYPDVSRMWNIVDKYKVTKLYTSPTAIRALMksgDEP-VKKCSreSLKI 415
Cdd:PRK05691 1320 KAPISFDVS-VWECFWPLITGCRLVL-AGPGEHRDPQRIAELVQQYGVTTLHFVPPLLQLFI---DEPlAAACT--SLRR 1392
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 416 LGTVGEPINPE---------AWLWYYHVIGDERCPIVDTFWQTETGGHMLTPLPAvtamkpgsatfPFFGVVPAILDESG 486
Cdd:PRK05691 1393 LFSGGEALPAElrnrvlqrlPQVQLHNRYGPTETAINVTHWQCQAEDGERSPIGR-----------PLGNVLCRVLDAEL 1461
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 487 QELEGEAEGFLVFKQPwpGIMRTVYG----NYKRFETTYFKKfPG--YYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLL 560
Cdd:PRK05691 1462 NLLPPGVAGELCIGGA--GLARGYLGrpalTAERFVPDPLGE-DGarLYRTGDRARWNADGALEYLGRLDQQVKLRGFRV 1538
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 561 STAEVESALAEHKAISEAAVVSHpHPVKGECLYCFVTLRDGHEFSktlQEELKKQVREKIgpiatPDYIQHA-----PGL 635
Cdd:PRK05691 1539 EPEEIQARLLAQPGVAQAAVLVR-EGAAGAQLVGYYTGEAGQEAE---AERLKAALAAEL-----PEYMVPAqlirlDQM 1609
|
570
....*....|...
gi 1394780554 636 PKTRSGKILRRVL 648
Cdd:PRK05691 1610 PLGPSGKLDRRAL 1622
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
287-653 |
2.38e-08 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 57.67 E-value: 2.38e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 287 DSEDELFILYTSGSTGKPKGVV--HTvggYMIFTAATFKYVFDYHPEDIYWCTGDIGwitgHSYltyGpLANGATSVLFE 364
Cdd:PRK06814 791 DPDDPAVILFTSGSEGTPKGVVlsHR---NLLANRAQVAARIDFSPEDKVFNALPVF----HSF---G-LTGGLVLPLLS 859
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 365 GVPTYpdvsrmwnivdkykvtkLYTSPTAIRA-----------LMKSGDEPVKKCSR-------ESLKILGTVGEPINPE 426
Cdd:PRK06814 860 GVKVF-----------------LYPSPLHYRIipeliydtnatILFGTDTFLNGYARyahpydfRSLRYVFAGAEKVKEE 922
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 427 A---WLWYYHVigdeRcpIVDTFWQTETGghmltPLPAV-TAM--KPGSATFPFFGV------VPAIlDESGQelegeae 494
Cdd:PRK06814 923 TrqtWMEKFGI----R--ILEGYGVTETA-----PVIALnTPMhnKAGTVGRLLPGIeyrlepVPGI-DEGGR------- 983
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 495 gfLVFKQPwpGIMRtvygNYKRFETTYFKKFP--GYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEH 572
Cdd:PRK06814 984 --LFVRGP--NVML----GYLRAENPGVLEPPadGWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAAEL 1055
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 573 KAISEAAVVSHPHPVKGECLYCFVTLRDGHefsktlQEELKKQVREK-IGPIATPDYIQHAPGLPKTRSGKILRRVLRQI 651
Cdd:PRK06814 1056 WPDALHAAVSIPDARKGERIILLTTASDAT------RAAFLAHAKAAgASELMVPAEIITIDEIPLLGTGKIDYVAVTKL 1129
|
..
gi 1394780554 652 AK 653
Cdd:PRK06814 1130 AE 1131
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
531-648 |
3.43e-08 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 56.20 E-value: 3.43e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 531 TGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRdgHEFSKtlqE 610
Cdd:PRK08308 295 TKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVISH--EEIDP---V 369
|
90 100 110
....*....|....*....|....*....|....*...
gi 1394780554 611 ELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVL 648
Cdd:PRK08308 370 QLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
270-650 |
5.34e-08 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 55.96 E-value: 5.34e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 270 WHELMRNASKECEPEWCDSEDEL-FILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVfDYHPEDIY--W--CTGDIGWIT 344
Cdd:cd05908 86 WNTLKNPYLITEEEVLCELADELaFIQFSSGSTGDPKGVMLTHENLVHNMFAILNST-EWKTKDRIlsWmpLTHDMGLIA 164
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 345 GHsyLTygPLANGATSVLfegVPTYPDVSR--MW-NIVDKYKVTKLYTSPTAIRALMKS-GDEPVKKCSRESLKILGTVG 420
Cdd:cd05908 165 FH--LA--PLIAGMNQYL---MPTRLFIRRpiLWlKKASEHKATIVSSPNFGYKYFLKTlKPEKANDWDLSSIRMILNGA 237
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 421 EPINPE---AWLWYYHVIGDERCPIVDTFWQTET---------GGHMLT------------PLPAVTAMKPGSATFPFFG 476
Cdd:cd05908 238 EPIDYElchEFLDHMSKYGLKRNAILPVYGLAEAsvgaslpkaQSPFKTitlgrrhvthgePEPEVDKKDSECLTFVEVG 317
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 477 VVPA-----ILDESGQELEGEAEGFLVFKQpwPGIMRTVYGNYKrfETTYFKKFPGYYVTGD-GCRRdkDGYYWITGRID 550
Cdd:cd05908 318 KPIDetdirICDEDNKILPDGYIGHIQIRG--KNVTPGYYNNPE--ATAKVFTDDGWLKTGDlGFIR--NGRLVITGREK 391
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 551 DMLNVSGHLLSTAEVESALAEHKAISEAAVVS---HPHPVKGECLYCFVTLRDGHEFSKTLQEELKKQVREKIG------ 621
Cdd:cd05908 392 DIIFVNGQNVYPHDIERIAEELEGVELGRVVAcgvNNSNTRNEEIFCFIEHRKSEDDFYPLGKKIKKHLNKRGGwqinev 471
|
410 420 430
....*....|....*....|....*....|
gi 1394780554 622 -PIATpdyiqhapgLPKTRSGKILRRVLRQ 650
Cdd:cd05908 472 lPIRR---------IPKTTSGKVKRYELAQ 492
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
526-656 |
6.37e-08 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 55.39 E-value: 6.37e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 526 PGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAVVSHPHPVKGECLYCFVTLRDGhefs 605
Cdd:PRK07445 323 QGIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDP---- 398
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|.
gi 1394780554 606 KTLQEELKKQVREKIGPIATPDYIQHAPGLPKTRSGKILRRVLRQIAKNDR 656
Cdd:PRK07445 399 SISLEELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQIAVQRL 449
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
118-317 |
1.35e-07 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 54.82 E-value: 1.35e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 118 TYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARigalHSIVfagfsaeslCERILDSNCSLLVtada 197
Cdd:PLN02430 78 TYKEVYEEVLQIGSALRASGAEPGSRVGIYGSNCPQWIVAMEACAA----HSLI---------CVPLYDTLGPGAV---- 140
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 198 fyrgDKLISLKQIADDALQKCKEKGFLVRNCIVVNHLgrEELVSdgTTSQSPPVKRLCQDVQMPWNSdvdlwWHELMR-- 275
Cdd:PLN02430 141 ----DYIVDHAEIDFVFVQDKKIKELLEPDCKSAKRL--KAIVS--FTSVTEEESDKASQIGVKTYS-----WIDFLHmg 207
|
170 180 190 200
....*....|....*....|....*....|....*....|....
gi 1394780554 276 --NASKECEPEwcdSEDELFILYTSGSTGKPKGVVHTVGGYMIF 317
Cdd:PLN02430 208 keNPSETNPPK---PLDICTIMYTSGTSGDPKGVVLTHEAVATF 248
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
77-401 |
9.80e-07 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 51.82 E-value: 9.80e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 77 MKGSTTNICYNLLDRnvnEKKLGDKIAFY----WEGNDPGISMSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMII 152
Cdd:PRK09274 1 MMASMANIARHLPRA---AQERPDQLAVAvpggRGADGKLAYDELSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 153 ELVVAMLACARIGALHSIVFAGFSAESLCERILDSNcsllvtADAFYrGdklISLKQIAddalQKCKEKGFL-VRNCIVV 231
Cdd:PRK09274 78 EFFALTFALFKAGAVPVLVDPGMGIKNLKQCLAEAQ------PDAFI-G---IPKAHLA----RRLFGWGKPsVRRLVTV 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 232 nhlGREeLVSDGTTSQSPPVKrlcqdvqmpwnsdvdlwwhelmrNASKECEPEWCDSEDELFILYTSGSTGKPKGVVHTV 311
Cdd:PRK09274 144 ---GGR-LLWGGTTLATLLRD-----------------------GAAAPFPMADLAPDDMAAILFTSGSTGTPKGVVYTH 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 312 GgymIFTA--ATFKYVFDyhpediyWCTGDIGWITGHSYLTYGPlANGATSVLFEGVPTYP---DVSRMWNIVDKYKVTK 386
Cdd:PRK09274 197 G---MFEAqiEALREDYG-------IEPGEIDLPTFPLFALFGP-ALGMTSVIPDMDPTRPatvDPAKLFAAIERYGVTN 265
|
330
....*....|....*
gi 1394780554 387 LYTSPTAIRALMKSG 401
Cdd:PRK09274 266 LFGSPALLERLGRYG 280
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
116-308 |
1.06e-06 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 52.35 E-value: 1.06e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESLcERIL-DSNCSLLVT 194
Cdd:PRK10252 483 QFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRL-KMMLeDARPSLLIT 561
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 195 ADAFyrgdklislkqiaddalqkckekgflvrncivvnhLGReelVSDGttsqsPPVKRLCQDVqmPWnsdvdlwwhelm 274
Cdd:PRK10252 562 TADQ-----------------------------------LPR---FADV-----PDLTSLCYNA--PL------------ 584
|
170 180 190
....*....|....*....|....*....|....*
gi 1394780554 275 rnASKECEPEWCDS-EDELFILYTSGSTGKPKGVV 308
Cdd:PRK10252 585 --APQGAAPLQLSQpHHTAYIIFTSGSTGRPKGVM 617
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
280-363 |
1.86e-06 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 51.06 E-value: 1.86e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 280 ECEPEWCDS--EDELFILYTSGSTGKPKGVVHTVGGyMIFTAATF-KYVFDY-HPEDiywctgdigwitghSYLTYGPLA 355
Cdd:cd17639 77 ECSAIFTDGkpDDLACIMYTSGSTGNPKGVMLTHGN-LVAGIAGLgDRVPELlGPDD--------------RYLAYLPLA 141
|
90
....*....|..
gi 1394780554 356 N----GATSVLF 363
Cdd:cd17639 142 HifelAAENVCL 153
|
|
| PRK09188 |
PRK09188 |
serine/threonine protein kinase; Provisional |
568-660 |
5.52e-06 |
|
serine/threonine protein kinase; Provisional
Pssm-ID: 236400 [Multi-domain] Cd Length: 365 Bit Score: 48.99 E-value: 5.52e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 568 ALAEHKAISEAAVVSHPHPVKGECLYCFVTlRDGHEFSKTLQEELKKQvrekiGPIATPDYIQHAPGLPKTRSGKILRRV 647
Cdd:PRK09188 248 ALKSDPAVSDVAIALFSLPAKGVGLYAFVE-AELPADEKSLRARLAGA-----KPPKPPEHIQPVAALPRDADGTVRDDI 321
|
90
....*....|...
gi 1394780554 648 LRQIAKNDRDLGD 660
Cdd:PRK09188 322 LRLIAMNQIDELD 334
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
100-307 |
1.34e-05 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 48.35 E-value: 1.34e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGNdpgismSITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGalHSIVFAGFSAES 179
Cdd:PRK04813 17 DFPAYDYLGE------KLTYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGAVKAG--HAYIPVDVSSPA 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 180 lcERILD----SNCSLLVTADAFYRGD---KLISLKQIADDALQkckekgflvrncivvnhlgreelvsDGTTSQSPPVK 252
Cdd:PRK04813 89 --ERIEMiievAKPSLIIATEELPLEIlgiPVITLDELKDIFAT-------------------------GNPYDFDHAVK 141
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*
gi 1394780554 253 rlcqdvqmpwnsdvdlwwhelmrnaskecepewcdSEDELFILYTSGSTGKPKGV 307
Cdd:PRK04813 142 -----------------------------------GDDNYYIIFTSGTTGKPKGV 161
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
100-650 |
1.88e-05 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 48.24 E-value: 1.88e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 100 DKIAFYWEGNDPGISMSITYRQLLQDVCQFASVLQEQGiKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSA-- 177
Cdd:PRK05691 24 DRLALRFLADDPGEGVVLSYRDLDLRARTIAAALQARA-SFGDRAVLLFPSGPDYVAAFFGCLYAGVIAVPAYPPESArr 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 178 ---ESLCERILDSNCSLLVTADAFYrgdklislkqiadDALQkckekgflvrncivvnhlGREELVSDGttsqSPPVkrL 254
Cdd:PRK05691 103 hhqERLLSIIADAEPRLLLTVADLR-------------DSLL------------------QMEELAAAN----APEL--L 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 255 CQDVQMPwnSDVDLWWhelmrnaskecEPEwCDSEDELFILYTSGSTGKPKGVvHTVGGYMIFTAATFKYVF--DYHPED 332
Cdd:PRK05691 146 CVDTLDP--ALAEAWQ-----------EPA-LQPDDIAFLQYTSGSTALPKGV-QVSHGNLVANEQLIRHGFgiDLNPDD 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 333 IY--WCT--GDIGWITGhsylTYGPLANGATSVL-------------FEGVPTY-------PDVSrmwnivdkYKVTKLY 388
Cdd:PRK05691 211 VIvsWLPlyHDMGLIGG----LLQPIFSGVPCVLmspayflerplrwLEAISEYggtisggPDFA--------YRLCSER 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 389 TSPTAIRAL-------MKSGDEPVKKCSRESL-KILGTVGepINPEAWLWYY-------HVIGDERCPIV-------DTF 446
Cdd:PRK05691 279 VSESALERLdlsrwrvAYSGSEPIRQDSLERFaEKFAACG--FDPDSFFASYglaeatlFVSGGRRGQGIpaleldaEAL 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 447 WQTETGGHMLTPLPAVTAMKPGSATFPffgVVPAILDESGQELEGE--AEGflvfkqpwPGImrtVYGNYKRFETT--YF 522
Cdd:PRK05691 357 ARNRAEPGTGSVLMSCGRSQPGHAVLI---VDPQSLEVLGDNRVGEiwASG--------PSI---AHGYWRNPEASakTF 422
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 523 KKFPG--YYVTGD-GCRRDkdGYYWITGRIDDMLNVSGHLLSTAEVESALAEhkaisEAAVVShphpvKGEcLYCFVTLR 599
Cdd:PRK05691 423 VEHDGrtWLRTGDlGFLRD--GELFVTGRLKDMLIVRGHNLYPQDIEKTVER-----EVEVVR-----KGR-VAAFAVNH 489
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1394780554 600 DGH-------EFSKTLQEELKKQVREKIGPIATPDYIQHAP---------GLPKTRSGKILRRVLRQ 650
Cdd:PRK05691 490 QGEegigiaaEISRSVQKILPPQALIKSIRQAVAEACQEAPsvvlllnpgALPKTSSGKLQRSACRL 556
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
117-199 |
1.89e-05 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 47.73 E-value: 1.89e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 117 ITYRQLLQDVCQFASVLQEQGIKKGDRISIYMPMIIELVVAMLACARIGALHSIVFAGFSAESL--CERIldSNCSLLVT 194
Cdd:cd05940 4 LTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLahCLNV--SSAKHLVV 81
|
....*
gi 1394780554 195 ADAFY 199
Cdd:cd05940 82 DAALY 86
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
294-355 |
1.01e-04 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 45.28 E-value: 1.01e-04
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1394780554 294 ILYTSGSTGKPKGVVHTVGGYMIFTAATFKYVFDYHPEDiywcTGDIgwitghsYLTYGPLA 355
Cdd:cd05927 119 ICYTSGTTGNPKGVMLTHGNIVSNVAGVFKILEILNKIN----PTDV-------YISYLPLA 169
|
|
| PaaK |
COG1541 |
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ... |
297-650 |
1.37e-04 |
|
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];
Pssm-ID: 441150 [Multi-domain] Cd Length: 423 Bit Score: 44.75 E-value: 1.37e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 297 TSGSTGKPKGVVHT-----VGGYM---IFTAATFkyvfdyHPEDIYWCTGDIGWITGHSYLTYGPLANGATSVLFEGVPT 368
Cdd:COG1541 91 SSGTTGKPTVVGYTrkdldRWAELfarSLRAAGV------RPGDRVQNAFGYGLFTGGLGLHYGAERLGATVIPAGGGNT 164
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 369 ypdvSRMWNIVDKYKVTKLYTSPTAIRALMKSGDEPVKKCSRESLK--ILGtvGEPInPEAW------LWyyhvigdeRC 440
Cdd:COG1541 165 ----ERQLRLMQDFGPTVLVGTPSYLLYLAEVAEEEGIDPRDLSLKkgIFG--GEPW-SEEMrkeieeRW--------GI 229
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 441 PIVDTFWQTETGGHMLTPLPAVTAMKPGSATFpffgvVPAILD-ESGQEL-EGEaEGFLVFkqpwpgimrtvygnykrfe 518
Cdd:COG1541 230 KAYDIYGLTEVGPGVAYECEAQDGLHIWEDHF-----LVEIIDpETGEPVpEGE-EGELVV------------------- 284
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 519 TTYFKKfpGY----YVTGD---------GCRR-----DKdgyywITGRIDDMLNVSGHLLSTAEVESALAEHKAISEA-- 578
Cdd:COG1541 285 TTLTKE--AMplirYRTGDltrllpepcPCGRthpriGR-----ILGRADDMLIIRGVNVFPSQIEEVLLRIPEVGPEyq 357
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1394780554 579 AVVSHPHP-----VKgeclycfVTLRDGHEFSKtLQEELKKQVREKIGpiATPDYIQHAPG-LPktRSGKILRRVLRQ 650
Cdd:COG1541 358 IVVDREGGldeltVR-------VELAPGASLEA-LAEAIAAALKAVLG--LRAEVELVEPGsLP--RSEGKAKRVIDR 423
|
|
| Dip2 |
cd05905 |
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of ... |
116-161 |
4.60e-04 |
|
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of the adenylate forming enzyme family, including insect luciferase, acetyl CoA ligases and the adenylation domain of nonribosomal peptide synthetases (NRPS). However, its function may have diverged from other members of the superfamily. In mouse embryo, Dip2 homolog A plays an important role in the development of both vertebrate and invertebrate nervous systems. Dip2A appears to regulate cell growth and the arrangement of cells in organs. Biochemically, Dip2A functions as a receptor of FSTL1, an extracellular glycoprotein, and may play a role as a cardiovascular protective agent.
Pssm-ID: 341231 [Multi-domain] Cd Length: 571 Bit Score: 43.49 E-value: 4.60e-04
10 20 30 40
....*....|....*....|....*....|....*....|....*..
gi 1394780554 116 SITYRQLLQDVCQFASVLQEQ-GIKKGDRISIYMPMIIELVVAMLAC 161
Cdd:cd05905 14 TLTWGKLLSRAEKIAAVLQKKvGLKPGDRVALMYPDPLDFVAAFYGC 60
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
289-355 |
1.16e-03 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 42.01 E-value: 1.16e-03
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1394780554 289 EDELFILYTSGSTGKPKGVVHTVGGyMIFTAATFKYVFDYHPEDIywctgdigwitghsYLTYGPLA 355
Cdd:PLN02736 221 EDVATICYTSGTTGTPKGVVLTHGN-LIANVAGSSLSTKFYPSDV--------------HISYLPLA 272
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
522-629 |
9.57e-03 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 39.20 E-value: 9.57e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394780554 522 FKKFPGYYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALAEHKAISEAAV--VSHP-HpvKGECLYCFVTL 598
Cdd:cd05938 381 FKKGDVYFNTGDLLVQDQQNFLYFHDRVGDTFRWKGENVATTEVADVLGLLDFLQEVNVygVTVPgH--EGRIGMAAVKL 458
|
90 100 110
....*....|....*....|....*....|.
gi 1394780554 599 RDGHEFSktlQEELKKQVREKIGPIATPDYI 629
Cdd:cd05938 459 KPGHEFD---GKKLYQHVREYLPAYARPRFL 486
|
|
| |
