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Conserved domains on  [gi|302412955|ref|XP_003004310|]
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7-dehydrocholesterol reductase [Verticillium alfalfae VaMs.102]

Protein Classification

phosphatidylethanolamine N-methyltransferase family domain-containing protein( domain architecture ID 229533)

phosphatidylethanolamine N-methyltransferase (PEMT) family domain-containing protein similar to Homo sapiens PEMT, which catalyzes the three sequential steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME), PMME to phosphatidyldimethylethanolamine (PDME), and PDME to phosphatidylcholine (PC)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ICMT super family cl21511
Isoprenylcysteine carboxyl methyltransferase (ICMT) family; The isoprenylcysteine ...
 72-399 3.53e-51

Isoprenylcysteine carboxyl methyltransferase (ICMT) family; The isoprenylcysteine o-methyltransferase (EC:2.1.1.100) family carry out carboxyl methylation of cleaved eukaryotic proteins that terminate in a CaaX motif. In Saccharomyces cerevisiae this methylation is carried out by Ste14p, an integral endoplasmic reticulum membrane protein. Ste14p is the founding member of the isoprenylcysteine carboxyl methyltransferase (ICMT) family, whose members share significant sequence homology.


The actual alignment was detected with superfamily member pfam01222:

Pssm-ID: 473892  Cd Length: 429  Bit Score: 177.25  E-value: 3.53e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955   72 TTYAAWVLSQAALYHALPGPLHRGPRTPGGRQLLYRLNGFYAWILTIGITAVATFCRLIDPTYIARHWGDLLATANVYCV 151
Cdd:pfam01222  72 TVFLLWYFFQAVFYLTLPGKVVEGLPLSNGRKLPYKINAFWSFLLTLAAIGVLHYTQLFELTYLYDNFVQIMSSAILFSF 151

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955  152 ALIVIFYVKARLKPDNAGETL--LTGHFWYDLFNGGELHPRTGvLFDWKHFNASRTGgLLLWTLM--------------- 214
Cdd:pfam01222 152 ALAIYLYVRSLKAPEEDKDAPggNSGNLIYDFFIGRELNPRIG-SLDIKMFFELRPG-LLGWVFInlaallkqyetygyv 229

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955  215 ------------------------FFETLDGAHERFSFYSIYGFAAIMPQIWTLQTQYLVRYPVDLS-NAMAIAVTTAFA 269
Cdd:pfam01222 230 tpsllfvllnqllyvfdglkneeaVLTTMDITTDGFGFMLAFGDLVWVPFTYSLQTRYLSVHPSELGwSTYAVAIYALLL 309

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955  270 AGWALNHDANRQKSKCRETAgnYLIWGKEAQFLeatyQTADGKKhrtvLLHSGWWGIVRHANYVGSLLYTWASCAACGAG 349
Cdd:pfam01222 310 CGYYIFRSANSQKNNFRTNP--ADPKLIYLKYI----QTKTGSG----LLTDGWWGFARHINYLGDWLQSLSWSLPTGFN 379

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|
gi 302412955  350 HFFPYTEALLVTATVIHRCFRDEARCKEKYGDTWDEYCRIVKWRMIPGIF 399
Cdd:pfam01222 380 SVLPYFYPLYFAVLLVHREARDEHKCKKKYGLDWEEYCKRVPYHIIPYVY 429
 
Name Accession Description Interval E-value
ERG4_ERG24 pfam01222
Ergosterol biosynthesis ERG4/ERG24 family;
72-399 3.53e-51

Ergosterol biosynthesis ERG4/ERG24 family;


Pssm-ID: 250456  Cd Length: 429  Bit Score: 177.25  E-value: 3.53e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955   72 TTYAAWVLSQAALYHALPGPLHRGPRTPGGRQLLYRLNGFYAWILTIGITAVATFCRLIDPTYIARHWGDLLATANVYCV 151
Cdd:pfam01222  72 TVFLLWYFFQAVFYLTLPGKVVEGLPLSNGRKLPYKINAFWSFLLTLAAIGVLHYTQLFELTYLYDNFVQIMSSAILFSF 151

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955  152 ALIVIFYVKARLKPDNAGETL--LTGHFWYDLFNGGELHPRTGvLFDWKHFNASRTGgLLLWTLM--------------- 214
Cdd:pfam01222 152 ALAIYLYVRSLKAPEEDKDAPggNSGNLIYDFFIGRELNPRIG-SLDIKMFFELRPG-LLGWVFInlaallkqyetygyv 229

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955  215 ------------------------FFETLDGAHERFSFYSIYGFAAIMPQIWTLQTQYLVRYPVDLS-NAMAIAVTTAFA 269
Cdd:pfam01222 230 tpsllfvllnqllyvfdglkneeaVLTTMDITTDGFGFMLAFGDLVWVPFTYSLQTRYLSVHPSELGwSTYAVAIYALLL 309

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955  270 AGWALNHDANRQKSKCRETAgnYLIWGKEAQFLeatyQTADGKKhrtvLLHSGWWGIVRHANYVGSLLYTWASCAACGAG 349
Cdd:pfam01222 310 CGYYIFRSANSQKNNFRTNP--ADPKLIYLKYI----QTKTGSG----LLTDGWWGFARHINYLGDWLQSLSWSLPTGFN 379

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|
gi 302412955  350 HFFPYTEALLVTATVIHRCFRDEARCKEKYGDTWDEYCRIVKWRMIPGIF 399
Cdd:pfam01222 380 SVLPYFYPLYFAVLLVHREARDEHKCKKKYGLDWEEYCKRVPYHIIPYVY 429
STE14 COG2020
Protein-S-isoprenylcysteine O-methyltransferase Ste14 [Posttranslational modification, protein ...
 313-398 2.51e-06

Protein-S-isoprenylcysteine O-methyltransferase Ste14 [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441623 [Multi-domain]  Cd Length: 113  Bit Score: 45.92  E-value: 2.51e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955 313 KHRTVLLHSGWWGIVRHANYVGSLLYTWASCAACGAGHFFPYteALLVTATVIHRCFRDEARCKEKYGDTWDEYCRIVKw 392
Cdd:COG2020   31 RKAHRLVTTGPYRYVRHPMYLGFLLLLLGLALLLGSLLALLL--ALLLLLLYVLRIRREERRLRARFGEEYRAYAARVP- 107


                 ....*.
gi 302412955 393 RMIPGI 398
Cdd:COG2020  108 RLIPRL 113
 
Name Accession Description Interval E-value
ERG4_ERG24 pfam01222
Ergosterol biosynthesis ERG4/ERG24 family;
72-399 3.53e-51

Ergosterol biosynthesis ERG4/ERG24 family;


Pssm-ID: 250456  Cd Length: 429  Bit Score: 177.25  E-value: 3.53e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955   72 TTYAAWVLSQAALYHALPGPLHRGPRTPGGRQLLYRLNGFYAWILTIGITAVATFCRLIDPTYIARHWGDLLATANVYCV 151
Cdd:pfam01222  72 TVFLLWYFFQAVFYLTLPGKVVEGLPLSNGRKLPYKINAFWSFLLTLAAIGVLHYTQLFELTYLYDNFVQIMSSAILFSF 151

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955  152 ALIVIFYVKARLKPDNAGETL--LTGHFWYDLFNGGELHPRTGvLFDWKHFNASRTGgLLLWTLM--------------- 214
Cdd:pfam01222 152 ALAIYLYVRSLKAPEEDKDAPggNSGNLIYDFFIGRELNPRIG-SLDIKMFFELRPG-LLGWVFInlaallkqyetygyv 229

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955  215 ------------------------FFETLDGAHERFSFYSIYGFAAIMPQIWTLQTQYLVRYPVDLS-NAMAIAVTTAFA 269
Cdd:pfam01222 230 tpsllfvllnqllyvfdglkneeaVLTTMDITTDGFGFMLAFGDLVWVPFTYSLQTRYLSVHPSELGwSTYAVAIYALLL 309

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955  270 AGWALNHDANRQKSKCRETAgnYLIWGKEAQFLeatyQTADGKKhrtvLLHSGWWGIVRHANYVGSLLYTWASCAACGAG 349
Cdd:pfam01222 310 CGYYIFRSANSQKNNFRTNP--ADPKLIYLKYI----QTKTGSG----LLTDGWWGFARHINYLGDWLQSLSWSLPTGFN 379

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|
gi 302412955  350 HFFPYTEALLVTATVIHRCFRDEARCKEKYGDTWDEYCRIVKWRMIPGIF 399
Cdd:pfam01222 380 SVLPYFYPLYFAVLLVHREARDEHKCKKKYGLDWEEYCKRVPYHIIPYVY 429
STE14 COG2020
Protein-S-isoprenylcysteine O-methyltransferase Ste14 [Posttranslational modification, protein ...
 313-398 2.51e-06

Protein-S-isoprenylcysteine O-methyltransferase Ste14 [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441623 [Multi-domain]  Cd Length: 113  Bit Score: 45.92  E-value: 2.51e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 302412955 313 KHRTVLLHSGWWGIVRHANYVGSLLYTWASCAACGAGHFFPYteALLVTATVIHRCFRDEARCKEKYGDTWDEYCRIVKw 392
Cdd:COG2020   31 RKAHRLVTTGPYRYVRHPMYLGFLLLLLGLALLLGSLLALLL--ALLLLLLYVLRIRREERRLRARFGEEYRAYAARVP- 107


                 ....*.
gi 302412955 393 RMIPGI 398
Cdd:COG2020  108 RLIPRL 113
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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