Paraslipin or slipin-2 (SLP-2, a subgroup of the stomatin-like proteins (slipins) family; belonging to the SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily; This model summarizes a subgroup of the stomatin-like protein family (SLPs or slipins) that is found in all three kingdoms of life. The conserved domain common to these families has also been referred to as the Band 7 domain. Individual proteins of the SPFH family may cluster to form membrane microdomains which may in turn recruit multiprotein complexes. This subgroup of the SLPs remains largely uncharacterized. It includes human SLP-2 which is upregulated and involved in the progression and development in several types of cancer, including esophageal squamous cell carcinoma, endometrial adenocarcinoma, breast cancer, and glioma.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480  81 RSVHSIKESVLEIQPHNCITIDQKDLIIDGVAFIKILDTFKATYNIDDVDFAINELCQTRMRTEIGNLKFDDVVKNRNEL 160
Cdd:cd08829    1 AYKVDLREQVLDIPPQEVITKDNVTVTVDAVLYYRVVDPYKASYGVEDLEYAIENLAQTTLRSEIGKMELDETLSSREEI 80

                         90       100
                 ....*....|....*....|....*....
gi 255725480 161 NEKIKDFINSASlENWGVECIRYEIKDIS 189
Cdd:cd08829   81 NAKLLEALDEAT-DPWGVKVTRVEIKDIT 108

SPFH domain / Band 7 family; This family has been called SPFH, Band 7 or PHB domain. Recent phylogenetic analysis has shown this domain to be a slipin or Stomatin-like integral membrane domain conserved from protozoa to mammals.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480   48 VPQQQQWVIERMGKYNRTVKEGPHLIIPIFEKIRSVhSIKESVLEIQPHNCITIDQKDLIIDGVAFIKIL--DTFKATYN 125
Cdd:pfam01145   3 VPPGEVGVVTRFGKLSRVLEPGLHFIIPFIQRVVTV-DVRVQTLEVSVQTVLTKDGVPVNVDVTVIYRVNpdDPPKLVQN 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 255725480  126 I---DDVDFAINELCQTRMRTEIGNLKFDDVVKNRNELNEKIKDFINSAsLENWGVECIRYEIKDIS 189
Cdd:pfam01145  82 VfgsDDLQELLRRVLESALREIIARYTLEELLSNREELAEEIKNALQEE-LAKYGVEIIDVQITDID 147

Paraslipin or slipin-2 (SLP-2, a subgroup of the stomatin-like proteins (slipins) family; belonging to the SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily; This model summarizes a subgroup of the stomatin-like protein family (SLPs or slipins) that is found in all three kingdoms of life. The conserved domain common to these families has also been referred to as the Band 7 domain. Individual proteins of the SPFH family may cluster to form membrane microdomains which may in turn recruit multiprotein complexes. This subgroup of the SLPs remains largely uncharacterized. It includes human SLP-2 which is upregulated and involved in the progression and development in several types of cancer, including esophageal squamous cell carcinoma, endometrial adenocarcinoma, breast cancer, and glioma.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480  81 RSVHSIKESVLEIQPHNCITIDQKDLIIDGVAFIKILDTFKATYNIDDVDFAINELCQTRMRTEIGNLKFDDVVKNRNEL 160
Cdd:cd08829    1 AYKVDLREQVLDIPPQEVITKDNVTVTVDAVLYYRVVDPYKASYGVEDLEYAIENLAQTTLRSEIGKMELDETLSSREEI 80

                         90       100
                 ....*....|....*....|....*....
gi 255725480 161 NEKIKDFINSASlENWGVECIRYEIKDIS 189
Cdd:cd08829   81 NAKLLEALDEAT-DPWGVKVTRVEIKDIT 108

Alloslipin, a subgroup of the stomatin-like proteins (slipins) family; belonging to the SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily; This model summarizes a subgroup of the stomatin-like protein family (SLPs or slipins) that is found in some eukaryotes and viruses. The conserved domain common to the SPFH superfamily has also been referred to as the Band 7 domain. Individual proteins of the SPFH superfamily may cluster to form membrane microdomains which may in turn recruit multiprotein complexes. This diverse subgroup of the SLPs remains largely uncharacterized.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480  48 VPQQQQWVIERMGKYNRTVKEGPHLIIPIFEKIRSVhSIKESVLEIQPHNCITIDQKDLIIDGVAFIKILDTFKATYNID 127
Cdd:cd13437    9 VKQGSVGLVERFGKFYKTVDPGLHKVNPCTEKIIQV-DMKTQVIDLPRQSVMTKDNVSVTIDSVVYYRIIDPYKAIYRID 87

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 255725480 128 DVDFAINELCQTRMRTEIGNLKFDDVVKNRNELNEKIKDFINSASlENWGVECIRYEIKDIS 189
Cdd:cd13437   88 NVKQALIERTQTTLRSVIGERTLQDLLEKREEIADEIEEIVEEVA-KEWGVYVESILIKDIV 148

Stomatin-like proteins (slipins) family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily; This model summarizes proteins similar to stomatin, podocin, and other members of the stomatin-like protein family (SLPs or slipins). The conserved domain common to the SPFH superfamily has also been referred to as the Band 7 domain. Individual proteins of the SPFH superfamily may cluster to form membrane microdomains which may in turn recruit multiprotein complexes. Stomatin interacts with and regulates members of the degenerin/epithelia Na+ channel family in mechanosensory cells of Caenorhabditis elegans and vertebrate neurons and participates in trafficking of Glut1 glucose transporters. Mutations in the podocin gene give rise to autosomal recessive steroid resistant nephritic syndrome. Bacterial and archaebacterial SLPs and many of the eukaryotic family members remain uncharacterized.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480  86 IKESVLEIQPHNCITIDQKDLIIDGVAFIKILDTFKATYNIDDVDFAINELCQTRMRTEIGNLKFDDVVKNRNELNEKIK 165
Cdd:cd13434    3 LRTQSVDVPPQEILTKDNVTVSVDAVVYYRVVDPLKAVLNVEDYKKATELLAQTTLRNVLGTRTLDELLSEREEISQQLQ 82

                         90       100
                 ....*....|....*....|....
gi 255725480 166 DFINSASLEnWGVECIRYEIKDIS 189
Cdd:cd13434   83 EILDEATDP-WGIKVERVEIKDII 105

Slipin-3 (SLP-3), an uncharacterized subgroup of the stomatin-like proteins (slipins) family; belonging to the SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily; This model summarizes a subgroup of the stomatin-like protein family (SLPs or slipins) that is found in vertebrates. The conserved domain common to the SPFH superfamily has also been referred to as the Band 7 domain. Individual proteins of the SPFH superfamily may cluster to form membrane microdomains which may in turn recruit multiprotein complexes. Members of this slipin subgroup remain uncharacterized, except for Caenorhabditis elegans UNC-1. Mutations in the unc-1 gene result in abnormal motion and altered patterns of sensitivity to volatile anesthetics.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480  93 IQPHNCITIDQKDLIIDGVAFIKILDTFKATYNIDDVDFAINELCQTRMRTEIGNLKFDDVVKNRNELNEKIKDFINSAS 172
Cdd:cd08828   27 IPPQEILTKDSVTTQVDGVVYYRIQSAVKAVANVNNVHIATFLLAQTTLRNVLGTQTLAQILAGREEIAHSIQSILDHAT 106

                         90
                 ....*....|....*.
gi 255725480 173 lENWGVECIRYEIKDI 188
Cdd:cd08828  107 -EKWGIKVARVEIKDV 121

High frequency of lysogenization C (HflC) family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily; This model characterizes proteins similar to prokaryotic HflC (High frequency of lysogenization C). Although many members of the SPFH (or band 7) superfamily are lipid raft associated, prokaryote plasma membranes lack cholesterol and are unlikely to have lipid raft domains. Individual proteins of this SPFH domain superfamily may cluster to form membrane microdomains which may in turn recruit multiprotein complexes. Escherichia coli HflC is an integral membrane protein which may localize to the plasma membrane. HflC associates with another SPFH superfamily member (HflK) to form an HflKC complex. HflKC interacts with FtsH in a large complex termed the FtsH holo-enzyme. FtsH is an AAA ATP-dependent protease which exerts progressive proteolysis against membrane-embedded and soluble substrate proteins. HflKC can modulate the activity of FtsH. HflKC plays a role in the decision between lysogenic and lytic cycle growth during lambda phage infection.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480  48 VPQQQQWVIERMGKYNRTVKE-GPHLIIPIFEKIRSVhSIKESVLEIQPHNCITIDQKDLIIDgvAFIK--ILDT---FK 121
Cdd:cd03405    5 VDETEQAVVLQFGKPVRVITEpGLHFKLPFIQNVRKF-DKRILTLDGPPEEVLTKDKKRLIVD--SYARwrITDPlrfYQ 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 255725480 122 ATYNIDDVDFAINELCQTRMRTEIGNLKFDDVV-KNRNELNEKIKDFINSAsLENWGVE 179
Cdd:cd03405   82 SVGGEEGAESRLDDIVDSALRNEIGKRTLAEVVsGGRDELMEEILEQANEE-AKEYGIE 139

Slipin-4 (SLP-4), an uncharacterized subgroup of the stomatin-like proteins (slipins) family; belonging to the SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily; This model summarizes a subgroup of the stomatin-like protein family (SLPs or slipins) that is found in arthropods. The conserved domain common to the SPFH superfamily has also been referred to as the Band 7 domain. Individual proteins of the SPFH superfamily may cluster to form membrane microdomains which may in turn recruit multiprotein complexes. Members of this divergent slipin subgroup remain largely uncharacterized. It contains Drosophila Mec2, the gene for which was identified in a screen for genes required for nephrocyte function; it may function together with Sns in maintaining nephrocyte diaphragm.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480  69 GPHLIIPIFEKIRSVhSIKESVLEIQPHNCITIDQKDLIIDGVAFIKILDTFKATYNIDDVDFAINELCQTRMRTEIGNL 148
Cdd:cd13435    8 GVFFVLPCIDNYCKV-DLRTVSFDVPPQEVLTKDSVTVTVDAVVYYRISDPLNAVIQVANYSHSTRLLAATTLRNVLGTR 86

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 255725480 149 KFDDVVKNRNELNEKIKDFINSASlENWGVECIRYEIKDIS 189
Cdd:cd13435   87 NLSELLTERETISHSMQVTLDEAT-DPWGVQVERVEIKDVS 126

Uncharacterized family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily; This model summarizes an uncharacterized family of proteins similar to stomatin, prohibitin, flotillin, HflK/C (SPFH) and podocin. The conserved domain common to the SPFH superfamily has also been referred to as the Band 7 domain. Many superfamily members are associated with lipid rafts. Individual proteins of the SPFH superfamily may cluster to form membrane microdomains which may in turn recruit multiprotein complexes. Microdomains formed from flotillin proteins may in addition be dynamic units with their own regulatory functions. Flotillins have been implicated in signal transduction, vesicle trafficking, cytoskeleton rearrangement and are known to interact with a variety of proteins. Stomatin interacts with and regulates members of the degenerin/epithelia Na+ channel family in mechanosensory cells of Caenorhabditis elegans and vertebrate neurons and participates in trafficking of Glut1 glucose transporters. Prohibitin may act as a chaperone for the stabilization of mitochondrial proteins. Prokaryotic HflK/C plays a role in the decision between lysogenic and lytic cycle growth during lambda phage infection. Flotillins have been implicated in the progression of prion disease, in the pathogenesis of neurodegenerative diseases such as Parkinson's and Alzheimer's disease and, in cancer invasion and metastasis. Mutations in the podocin gene give rise to autosomal recessive steroid resistant nephritic syndrome.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480  48 VPQQQQWVIERMGKYNRTVKEGPHLIIPIFEKIRSVHSIKESVLEIqphNCITiDQKD---LIIDGVAFIKILDT--FKA 122
Cdd:cd03407    2 VSQSTVAIVERFGKFSRIAEPGLHFIIPPIESVAGRVSLRVQQLDV---RVET-KTKDnvfVTLVVSVQYRVVPEkvYDA 77

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 255725480 123 TYNIDDVDFAINELCQTRMRTEIGNLKFDDVVKNRNELNEKIKDFInSASLENWGVECIRYEIKDI 188
Cdd:cd03407   78 FYKLTNPEQQIQSYVFDVVRASVPKLTLDEVFESKDEIAKAVKEEL-AKVMSEYGYEIVKTLVTDI 142

core domain of the SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily; This model summarizes proteins similar to stomatin, prohibitin, flotillin, HflK/C (SPFH) and podocin. The conserved domain common to the SPFH superfamily has also been referred to as the Band 7 domain. Many superfamily members are associated with lipid rafts. Individual proteins of the SPFH superfamily may cluster to form membrane microdomains which may in turn recruit multiprotein complexes. Microdomains formed from flotillin proteins may in addition be dynamic units with their own regulatory functions. Flotillins have been implicated in signal transduction, vesicle trafficking, cytoskeleton rearrangement and are known to interact with a variety of proteins. Stomatin interacts with and regulates members of the degenerin/epithelia Na+ channel family in mechanosensory cells of Caenorhabditis elegans and vertebrate neurons, and participates in trafficking of Glut1 glucose transporters. Prohibitin may act as a chaperone for the stabilization of mitochondrial proteins. Prokaryotic HflK/C plays a role in the decision between lysogenic and lytic cycle growth during lambda phage infection. Flotillins have been implicated in the progression of prion disease, in the pathogenesis of neurodegenerative diseases such as Parkinson's and Alzheimer's disease, and in cancer invasion and metastasis. Mutations in the podocin gene give rise to autosomal recessive steroid resistant nephritic syndrome.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480  88 ESVLEIQPHNCITIDQKDLIIDGVAFIKILDTF--KATYNIDDVDFAINELCQT---RMRTEIGNLKFDDVVKNRNELNE 162
Cdd:cd02106    2 PQFDDVRVEPVGTADGVPVAVDLVVQFRITDYNalPAFYLVDFVKDIKADIRRKiadVLRAAIGRMTLDQIISGRDEIAK 81

                         90       100
                 ....*....|....*....|....*..
gi 255725480 163 KIKDFINSAsLENWGVECIRYEIKDIS 189
Cdd:cd02106   82 AVKEDLEED-LENFGVVISDVDITSIE 107

Uncharacterized membrane protein YqiK, contains Band7/PHB/SPFH domain [Function unknown];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480  58 RMGKYnRTVKEGPHLIIPIFEKIRSVhSIKESVLEIQPH-NCITIDQKDLIIDGVAFIKILDTFKATYN---------ID 127
Cdd:COG2268   42 RGGGY-KVVTGGGAFVLPVLHRAERM-SLSTMTIEVERTeGLITKDGIRVDVDAVFYVKVNSDPEDIANaaerflgrdPE 119

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 255725480 128 DVDFAINELCQTRMRTEIGNLKFDDVVKNRNELNEKIKDFInSASLENWGVECIRYEIKDIS 189
Cdd:COG2268  120 EIEELAEEKLEGALRAVAAQMTVEELNEDREKFAEKVQEVA-GTDLAKNGLELESVAITDLE 180

Uncharacterized family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily; This model summarizes an uncharacterized family of proteins similar to stomatin, prohibitin, flotillin, HflK/C (SPFH) and podocin. The conserved domain common to the SPFH superfamily has also been referred to as the Band 7 domain. Many superfamily members are associated with lipid rafts. Individual proteins of the SPFH superfamily may cluster to form membrane microdomains which may in turn recruit multiprotein complexes. Microdomains formed from flotillin proteins may in addition be dynamic units with their own regulatory functions. Flotillins have been implicated in signal transduction, vesicle trafficking, cytoskeleton rearrangement and are known to interact with a variety of proteins. Stomatin interacts with and regulates members of the degenerin/epithelia Na+ channel family in mechanosensory cells of Caenorhabditis elegans and vertebrate neurons and participates in trafficking of Glut1 glucose transporters. Prohibitin may act as a chaperone for the stabilization of mitochondrial proteins. Prokaryotic HflK/C plays a role in the decision between lysogenic and lytic cycle growth during lambda phage infection. Flotillins have been implicated in the progression of prion disease, in the pathogenesis of neurodegenerative diseases such as Parkinson's and Alzheimer's disease, and in cancer invasion and metastasis. Mutations in the podocin gene give rise to autosomal recessive steroid resistant nephritic syndrome.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255725480  48 VPQQQQWVIERMGKYNRTVKE-GPHLIIP------IFEKIRSVHSIKESVLEIQ--PhncitidqkdLIIDGVAFIKILD 118
Cdd:cd03402   13 VQPNEAAVLTLFGRYRGTVRRpGLRWVNPfyrkkrVSLRVRNFESEPLKVNDANgnP----------IEIAAVVVWRVVD 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 255725480 119 TFKATYNIDDVDFAINELCQTRMRTEIGNLKFDDVVK-------NRNELNEKIKDFINSAsLENWGVECIRYEIKDIS 189
Cdd:cd03402   83 TAKAVFDVDDYEEFVSIQSEAALRRVASRYPYDSFEDgepslrgNSDEVSEELRRELQER-LAVAGVEVIEARITHLA 159