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Conserved domains on  [gi|154294644|ref|XP_001547762|]
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Bcole1 [Botrytis cinerea B05.10]

Protein Classification

acyl-CoA desaturase( domain architecture ID 10787620)

acyl-CoA desaturase containing a C-terminal cytochrome b5 heme/steroid-binding domain, utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates

CATH:  3.10.120.10
EC:  1.14.19.1
Gene Ontology:  GO:0004768|GO:0006636|GO:0020037|GO:0005506|GO:0009055
PubMed:  9767077|15189125|12440977
SCOP:  3000073

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
OLE1 COG1398
Fatty-acid desaturase [Lipid transport and metabolism];
44-305 8.83e-100

Fatty-acid desaturase [Lipid transport and metabolism];


:

Pssm-ID: 441008  Cd Length: 286  Bit Score: 300.59  E-value: 8.83e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644  44 VNWLNVTLIVGVPIYGLIMA---YWTPLQWKTAVWAVLYYFMTGLGITAGYHRLWAHSSYSATLPLKIFLAAVGGGAVEG 120
Cdd:COG1398   14 INWVTVLFFVLLHLLALLAAvpyAGVGFSWSAVALALVLYVLTGLGITVGYHRLFSHRSFKTPRWLEYLLAILGALALQG 93

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 121 SIRWWSRDHRAHHRYTDTDKDPYS-VRKGLLYSHFGWMIMKQNPKRIGRTdISDLNDDPVVVWQHRNYIKTVIFMGMVFP 199
Cdd:COG1398   94 GPLWWVADHRRHHRHSDTEGDPHSpKLRGFWWSHMGWMLREDPTPNDYRY-APDLAKDPELRWLDRYYLLLQLALGLLLP 172

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 200 cLVTGLGWGDWFGGFIYAGILRIFFVQQATFCVNSLAHWLGDQPFDDRNSPRDHVITALVTLGEGYHNFHHEFPSDYRNA 279
Cdd:COG1398  173 -ALLGGLLGGGWSGLLWGGFVRTVLLHHGTWFINSLAHVWGYRPFETRDTSRNNWWLALLTFGEGWHNNHHAFPTSARHG 251

                        250       260
                 ....*....|....*....|....*.
gi 154294644 280 IEWHQYDPTKWSIWMWKQLGLAYDLK 305
Cdd:COG1398  252 LRWWEIDPTWWLIRLLEKLGLAWDVR 277
Cyt-b5 pfam00173
Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from ...
 344-418 1.06e-18

Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.


:

Pssm-ID: 459698 [Multi-domain]  Cd Length: 74  Bit Score: 79.97  E-value: 1.06e-18
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 154294644  344 DWDTYQTEAKNGRALVAVAGVIHDVTDFVKEHPGGKALITSGIGKDATAIFNGgvYNHSN-AAHNLLSTMRVGVLR 418
Cdd:pfam00173   1 TLEELSKHNGDGDCWVAINGKVYDVTKFLKEHPGGEDVILSAAGKDATDAFEA--IGHSEdAAEKLLKKYRIGELA 74
 
Name Accession Description Interval E-value
OLE1 COG1398
Fatty-acid desaturase [Lipid transport and metabolism];
44-305 8.83e-100

Fatty-acid desaturase [Lipid transport and metabolism];


Pssm-ID: 441008  Cd Length: 286  Bit Score: 300.59  E-value: 8.83e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644  44 VNWLNVTLIVGVPIYGLIMA---YWTPLQWKTAVWAVLYYFMTGLGITAGYHRLWAHSSYSATLPLKIFLAAVGGGAVEG 120
Cdd:COG1398   14 INWVTVLFFVLLHLLALLAAvpyAGVGFSWSAVALALVLYVLTGLGITVGYHRLFSHRSFKTPRWLEYLLAILGALALQG 93

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 121 SIRWWSRDHRAHHRYTDTDKDPYS-VRKGLLYSHFGWMIMKQNPKRIGRTdISDLNDDPVVVWQHRNYIKTVIFMGMVFP 199
Cdd:COG1398   94 GPLWWVADHRRHHRHSDTEGDPHSpKLRGFWWSHMGWMLREDPTPNDYRY-APDLAKDPELRWLDRYYLLLQLALGLLLP 172

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 200 cLVTGLGWGDWFGGFIYAGILRIFFVQQATFCVNSLAHWLGDQPFDDRNSPRDHVITALVTLGEGYHNFHHEFPSDYRNA 279
Cdd:COG1398  173 -ALLGGLLGGGWSGLLWGGFVRTVLLHHGTWFINSLAHVWGYRPFETRDTSRNNWWLALLTFGEGWHNNHHAFPTSARHG 251

                        250       260
                 ....*....|....*....|....*.
gi 154294644 280 IEWHQYDPTKWSIWMWKQLGLAYDLK 305
Cdd:COG1398  252 LRWWEIDPTWWLIRLLEKLGLAWDVR 277
Delta9-FADS-like cd03505
The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like CD includes the delta-9 and delta-11 acyl ...
 69-305 4.18e-86

The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like CD includes the delta-9 and delta-11 acyl CoA desaturases found in various eukaryotes including vertebrates, insects, higher plants, and fungi. The delta-9 acyl-lipid desaturases are found in a wide range of bacteria. These enzymes play essential roles in fatty acid metabolism and the regulation of cell membrane fluidity. Acyl-CoA desaturases are the enzymes involved in the CoA-bound desaturation of fatty acids. Mammalian stearoyl-CoA delta-9 desaturase is a key enzyme in the biosynthesis of monounsaturated fatty acids, and in yeast, the delta-9 acyl-CoA desaturase (OLE1) reaction accounts for all de nova unsaturated fatty acid production in Saccharomyces cerevisiae. These non-heme, iron-containing, ER membrane-bound enzymes are part of a three-component enzyme system involving cytochrome b5, cytochrome b5 reductase, and the delta-9 fatty acid desaturase. This complex catalyzes the NADH- and oxygen-dependent insertion of a cis double bond between carbons 9 and 10 of the saturated fatty acyl substrates, palmitoyl (16:0)-CoA or stearoyl (18:0)-CoA, yielding the monoenoic products palmitoleic (16:l) or oleic (18:l) acids, respectively. In cyanobacteria, the biosynthesis of unsaturated fatty acids is initiated by delta 9 acyl-lipid desaturase (DesC) which introduces the first double bond at the delta-9 position of a saturated fatty acid that has been esterified to a glycerolipid. This domain family has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain the residues: HXXXXH, HXXHH, and H/QXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the rat stearoyl CoA delta-9 desaturase. Some eukaryotic (Fungi, Euglenozoa, Mycetozoa, Rhodophyta) desaturase domains have an adjacent C-terminal cytochrome b5-like domain.


Pssm-ID: 239582  Cd Length: 178  Bit Score: 261.34  E-value: 4.18e-86
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644  69 QWKTAVWAVLYYFMTGLGITAGYHRLWAHSSYSATLPLKIFLAAVGGGAVEGSIRWWSRDHRAHHRYTDTDKDPYSVRKG 148
Cdd:cd03505    1 SWATLVFLVLYYLLTGLGITAGYHRLWAHRSFKAPKPLRIFLAILGSLAGQGSPLWWVADHRLHHRYSDTDGDPHSPKRG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 149 LLYSHFGWMimkqnpkrigrtdisdlnddpvvvwqhrnyiktvifmgmvfpclvtglgwgdwfggfiyAGILRIFFVQQA 228
Cdd:cd03505   81 FWFSHVGWL-----------------------------------------------------------GGLLRIVLVLHA 101

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 154294644 229 TFCVNSLAHWLGDQPFDDRNSPRDHVITALVTLGEGYHNFHHEFPSDYRNAIEWHQYDPTKWSIWMWKQLGLAYDLK 305
Cdd:cd03505  102 TWLVNSLAHMWGYRPYDTRDTSRNNWWVALLTFGEGWHNNHHAFPGDARNGLKWYQIDPTKWVIRLLEKLGLAWDLK 178
PLN02220 PLN02220
delta-9 acyl-lipid desaturase
 69-305 3.57e-31

delta-9 acyl-lipid desaturase


Pssm-ID: 177866 [Multi-domain]  Cd Length: 299  Bit Score: 121.83  E-value: 3.57e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644  69 QWKTAVWAVLYYFMTGLGITAGYHRLWAHSSYSATLPLKIFLAAVGGGAVEGSIRWWSRDHRAHHRYTDTDKDPYSVRKG 148
Cdd:PLN02220  54 KWEALRFGLILYIVTGLSITFSYHRNLAHRSFKLPKWLEYPFAYSALFALQGDPIDWVSTHRFHHQFTDSDRDPHSPIEG 133

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 149 LLYSHFGWMIMKQ--NPKRIGRTDISDLNDDpvvvWQHRNYIKTVIFMGMVFPCLVTGLG------WGDWFGGFIyagil 220
Cdd:PLN02220 134 FWFSHVLWIFDTSyiREKCGGRDNVMDLKQQ----WFYRFLRKTIGLHILMFWTLLYLWGglpyltWGVGVGGAI----- 204

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 221 riffVQQATFCVNSLAHWLGDQPFDDRNSPRDHVITALVTLGEGYHNFHHEFPSDYRNAIEWHQYDPTKWSIWMWKQLGL 300
Cdd:PLN02220 205 ----GYHVTWLINSACHIWGSRTWKTKDTSRNVWWLSLFTMGESWHNNHHAFESSARQGLEWWQIDITWYLIRFFEVLGL 280


                 ....*
gi 154294644 301 AYDLK 305
Cdd:PLN02220 281 ATDVK 285
Cyt-b5 pfam00173
Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from ...
 344-418 1.06e-18

Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.


Pssm-ID: 459698 [Multi-domain]  Cd Length: 74  Bit Score: 79.97  E-value: 1.06e-18
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 154294644  344 DWDTYQTEAKNGRALVAVAGVIHDVTDFVKEHPGGKALITSGIGKDATAIFNGgvYNHSN-AAHNLLSTMRVGVLR 418
Cdd:pfam00173   1 TLEELSKHNGDGDCWVAINGKVYDVTKFLKEHPGGEDVILSAAGKDATDAFEA--IGHSEdAAEKLLKKYRIGELA 74
FA_desaturase pfam00487
Fatty acid desaturase; Fatty acid desaturases are enzymes that catalyze the insertion of a ...
 73-290 1.38e-12

Fatty acid desaturase; Fatty acid desaturases are enzymes that catalyze the insertion of a double bond at the delta position of fatty acids. There seem to be two distinct families of fatty acid desaturases which do not seem to be evolutionary related: Family 1 composed of Stearoyl-CoA desaturases (SCD) and Family 2 composed of Bacterial fatty acid desaturases, Plant stearoyl-acyl-carrier-protein desaturase and Cyanobacterial DesA. Members of this entry are ER integral membrane proteins that share the same mushroom-shaped fold consisting of four transmembrane helices (TM1-TM4) which anchor them to the membrane, capped by a cytosolic domain containing a unique 9-10 histidine- coordinating di metal (di-iron) catalytic centre. The structure of mouse stearoyl-CoA desaturase (SDC) revealed that TM2 and TM4 are longer than TM1 and TM3 and protrude into the cytosolic domain, providing three of the nine histidine residues that coordinate the two metal ions, while the other histidine residues are provided by the soluble domain in this enzyme.


Pssm-ID: 425713 [Multi-domain]  Cd Length: 252  Bit Score: 67.37  E-value: 1.38e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644   73 AVWAVLYYFMTGLGITAGYHRLWAHSSYSATLPLK----IFLAAVGGGAVEGSIRWWSRDHRAHHRYT-DTDKDPYSV-- 145
Cdd:pfam00487   4 ALLLALLLGLFLLGITGSLAHEASHGALFKKRRLNrwlnDLLGRLAGLPLGISYSAWRIAHLVHHRYTnGPDKDPDTApl 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644  146 ---RKGLLYSHFGWMIMKQN------PKRIGRTDISDLNDDPVVVWQHRNYIKTVIFMGMVFPCLVTGLGWGDWFGGFIY 216
Cdd:pfam00487  84 asrFRGLLRYLLRWLLGLLVlawllaLVLPLWLRRLARRKRPIKSRRRRWRLIAWLLLLAAWLGLWLGFLGLGGLLLLLW 163

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 154294644  217 AGILRIFFVQQATFcVNSLAH----WLGDQPFDDRNSPRDHVITALVTLGEGYHNFHHEFPsdyrnAIEWHQYDPTKW 290
Cdd:pfam00487 164 LLPLLVFGFLLALI-FNYLEHyggdWGERPVETTRSIRSPNWWLNLLTGNLNYHIEHHLFP-----GVPWYRLPKLHR 235
PLN02252 PLN02252
nitrate reductase [NADPH]
 361-417 1.15e-04

nitrate reductase [NADPH]


Pssm-ID: 215141 [Multi-domain]  Cd Length: 888  Bit Score: 44.67  E-value: 1.15e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 154294644 361 VAGVIHDVTDFVKEHPGGKALITSGIGKDATAIFNGgvyNHSNAAHNLLSTMRVGVL 417
Cdd:PLN02252 538 VHGHVYDCTRFLKDHPGGADSILINAGTDCTEEFDA---IHSDKAKKMLEDYRIGEL 591
 
Name Accession Description Interval E-value
OLE1 COG1398
Fatty-acid desaturase [Lipid transport and metabolism];
44-305 8.83e-100

Fatty-acid desaturase [Lipid transport and metabolism];


Pssm-ID: 441008  Cd Length: 286  Bit Score: 300.59  E-value: 8.83e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644  44 VNWLNVTLIVGVPIYGLIMA---YWTPLQWKTAVWAVLYYFMTGLGITAGYHRLWAHSSYSATLPLKIFLAAVGGGAVEG 120
Cdd:COG1398   14 INWVTVLFFVLLHLLALLAAvpyAGVGFSWSAVALALVLYVLTGLGITVGYHRLFSHRSFKTPRWLEYLLAILGALALQG 93

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 121 SIRWWSRDHRAHHRYTDTDKDPYS-VRKGLLYSHFGWMIMKQNPKRIGRTdISDLNDDPVVVWQHRNYIKTVIFMGMVFP 199
Cdd:COG1398   94 GPLWWVADHRRHHRHSDTEGDPHSpKLRGFWWSHMGWMLREDPTPNDYRY-APDLAKDPELRWLDRYYLLLQLALGLLLP 172

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 200 cLVTGLGWGDWFGGFIYAGILRIFFVQQATFCVNSLAHWLGDQPFDDRNSPRDHVITALVTLGEGYHNFHHEFPSDYRNA 279
Cdd:COG1398  173 -ALLGGLLGGGWSGLLWGGFVRTVLLHHGTWFINSLAHVWGYRPFETRDTSRNNWWLALLTFGEGWHNNHHAFPTSARHG 251

                        250       260
                 ....*....|....*....|....*.
gi 154294644 280 IEWHQYDPTKWSIWMWKQLGLAYDLK 305
Cdd:COG1398  252 LRWWEIDPTWWLIRLLEKLGLAWDVR 277
Delta9-FADS-like cd03505
The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like CD includes the delta-9 and delta-11 acyl ...
 69-305 4.18e-86

The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like CD includes the delta-9 and delta-11 acyl CoA desaturases found in various eukaryotes including vertebrates, insects, higher plants, and fungi. The delta-9 acyl-lipid desaturases are found in a wide range of bacteria. These enzymes play essential roles in fatty acid metabolism and the regulation of cell membrane fluidity. Acyl-CoA desaturases are the enzymes involved in the CoA-bound desaturation of fatty acids. Mammalian stearoyl-CoA delta-9 desaturase is a key enzyme in the biosynthesis of monounsaturated fatty acids, and in yeast, the delta-9 acyl-CoA desaturase (OLE1) reaction accounts for all de nova unsaturated fatty acid production in Saccharomyces cerevisiae. These non-heme, iron-containing, ER membrane-bound enzymes are part of a three-component enzyme system involving cytochrome b5, cytochrome b5 reductase, and the delta-9 fatty acid desaturase. This complex catalyzes the NADH- and oxygen-dependent insertion of a cis double bond between carbons 9 and 10 of the saturated fatty acyl substrates, palmitoyl (16:0)-CoA or stearoyl (18:0)-CoA, yielding the monoenoic products palmitoleic (16:l) or oleic (18:l) acids, respectively. In cyanobacteria, the biosynthesis of unsaturated fatty acids is initiated by delta 9 acyl-lipid desaturase (DesC) which introduces the first double bond at the delta-9 position of a saturated fatty acid that has been esterified to a glycerolipid. This domain family has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain the residues: HXXXXH, HXXHH, and H/QXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the rat stearoyl CoA delta-9 desaturase. Some eukaryotic (Fungi, Euglenozoa, Mycetozoa, Rhodophyta) desaturase domains have an adjacent C-terminal cytochrome b5-like domain.


Pssm-ID: 239582  Cd Length: 178  Bit Score: 261.34  E-value: 4.18e-86
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644  69 QWKTAVWAVLYYFMTGLGITAGYHRLWAHSSYSATLPLKIFLAAVGGGAVEGSIRWWSRDHRAHHRYTDTDKDPYSVRKG 148
Cdd:cd03505    1 SWATLVFLVLYYLLTGLGITAGYHRLWAHRSFKAPKPLRIFLAILGSLAGQGSPLWWVADHRLHHRYSDTDGDPHSPKRG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 149 LLYSHFGWMimkqnpkrigrtdisdlnddpvvvwqhrnyiktvifmgmvfpclvtglgwgdwfggfiyAGILRIFFVQQA 228
Cdd:cd03505   81 FWFSHVGWL-----------------------------------------------------------GGLLRIVLVLHA 101

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 154294644 229 TFCVNSLAHWLGDQPFDDRNSPRDHVITALVTLGEGYHNFHHEFPSDYRNAIEWHQYDPTKWSIWMWKQLGLAYDLK 305
Cdd:cd03505  102 TWLVNSLAHMWGYRPYDTRDTSRNNWWVALLTFGEGWHNNHHAFPGDARNGLKWYQIDPTKWVIRLLEKLGLAWDLK 178
PLN02220 PLN02220
delta-9 acyl-lipid desaturase
 69-305 3.57e-31

delta-9 acyl-lipid desaturase


Pssm-ID: 177866 [Multi-domain]  Cd Length: 299  Bit Score: 121.83  E-value: 3.57e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644  69 QWKTAVWAVLYYFMTGLGITAGYHRLWAHSSYSATLPLKIFLAAVGGGAVEGSIRWWSRDHRAHHRYTDTDKDPYSVRKG 148
Cdd:PLN02220  54 KWEALRFGLILYIVTGLSITFSYHRNLAHRSFKLPKWLEYPFAYSALFALQGDPIDWVSTHRFHHQFTDSDRDPHSPIEG 133

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 149 LLYSHFGWMIMKQ--NPKRIGRTDISDLNDDpvvvWQHRNYIKTVIFMGMVFPCLVTGLG------WGDWFGGFIyagil 220
Cdd:PLN02220 134 FWFSHVLWIFDTSyiREKCGGRDNVMDLKQQ----WFYRFLRKTIGLHILMFWTLLYLWGglpyltWGVGVGGAI----- 204

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 221 riffVQQATFCVNSLAHWLGDQPFDDRNSPRDHVITALVTLGEGYHNFHHEFPSDYRNAIEWHQYDPTKWSIWMWKQLGL 300
Cdd:PLN02220 205 ----GYHVTWLINSACHIWGSRTWKTKDTSRNVWWLSLFTMGESWHNNHHAFESSARQGLEWWQIDITWYLIRFFEVLGL 280


                 ....*
gi 154294644 301 AYDLK 305
Cdd:PLN02220 281 ATDVK 285
Cyt-b5 pfam00173
Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from ...
 344-418 1.06e-18

Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.


Pssm-ID: 459698 [Multi-domain]  Cd Length: 74  Bit Score: 79.97  E-value: 1.06e-18
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 154294644  344 DWDTYQTEAKNGRALVAVAGVIHDVTDFVKEHPGGKALITSGIGKDATAIFNGgvYNHSN-AAHNLLSTMRVGVLR 418
Cdd:pfam00173   1 TLEELSKHNGDGDCWVAINGKVYDVTKFLKEHPGGEDVILSAAGKDATDAFEA--IGHSEdAAEKLLKKYRIGELA 74
Membrane-FADS-like cd01060
The membrane fatty acid desaturase (Membrane_FADS)-like CD includes membrane FADSs, alkane ...
 74-273 1.79e-14

The membrane fatty acid desaturase (Membrane_FADS)-like CD includes membrane FADSs, alkane hydroxylases, beta carotene ketolases (CrtW-like), hydroxylases (CrtR-like), and other related proteins. They are present in all groups of organisms with the exception of archaea. Membrane FADSs are non-heme, iron-containing, oxygen-dependent enzymes involved in regioselective introduction of double bonds in fatty acyl aliphatic chains. They play an important role in the maintenance of the proper structure and functioning of biological membranes. Alkane hydroxylases are bacterial, integral-membrane di-iron enzymes that share a requirement for iron and oxygen for activity similar to that of membrane FADSs, and are involved in the initial oxidation of inactivated alkanes. Beta-carotene ketolase and beta-carotene hydroxylase are carotenoid biosynthetic enzymes for astaxanthin and zeaxanthin, respectively. This superfamily domain has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of these sequences also reveals three regions of conserved histidine cluster motifs that contain eight histidine residues: HXXX(X)H, HXX(X)HH, and HXXHH (an additional conserved histidine residue is seen between clusters 2 and 3). Spectroscopic and genetic evidence point to a nitrogen-rich coordination environment located in the cytoplasm with as many as eight histidines coordinating the two iron ions and a carboxylate residue bridging the two metals in the Pseudomonas oleovorans alkane hydroxylase (AlkB). In addition, the eight histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the rat stearoyl CoA delta-9 desaturase.


Pssm-ID: 238511 [Multi-domain]  Cd Length: 122  Bit Score: 69.81  E-value: 1.79e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644  74 VWAVLYYFMTGLGITAGYHRLwAHSSYSATLPLKIFLAAVGGGAVEGSIRWWSRDHRAHHRYTDTD-KDPYSVrkgllys 152
Cdd:cd01060    2 LLALLLGLLGGLGLTVLAHEL-GHRSFFRSRWLNRLLGALLGLALGGSYGWWRRSHRRHHRYTNTPgKDPDSA------- 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 153 hfgwmimkqnpkrigrtdisdlnddpvvvwqhrnyiktvifmgmvfpclvtglgwgdwfggfiyagilriffvqqatfcV 232
Cdd:cd01060   74 -------------------------------------------------------------------------------V 74

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 154294644 233 NSLAHWLGDQPFDD-------RNSPRDHVITALVTLGEGYHNFHHEFP 273
Cdd:cd01060   75 NYLEHYGGDRPFDTdgewlrtTDNSRNGWLNLLLTGGLGYHNEHHLFP 122
FA_desaturase pfam00487
Fatty acid desaturase; Fatty acid desaturases are enzymes that catalyze the insertion of a ...
 73-290 1.38e-12

Fatty acid desaturase; Fatty acid desaturases are enzymes that catalyze the insertion of a double bond at the delta position of fatty acids. There seem to be two distinct families of fatty acid desaturases which do not seem to be evolutionary related: Family 1 composed of Stearoyl-CoA desaturases (SCD) and Family 2 composed of Bacterial fatty acid desaturases, Plant stearoyl-acyl-carrier-protein desaturase and Cyanobacterial DesA. Members of this entry are ER integral membrane proteins that share the same mushroom-shaped fold consisting of four transmembrane helices (TM1-TM4) which anchor them to the membrane, capped by a cytosolic domain containing a unique 9-10 histidine- coordinating di metal (di-iron) catalytic centre. The structure of mouse stearoyl-CoA desaturase (SDC) revealed that TM2 and TM4 are longer than TM1 and TM3 and protrude into the cytosolic domain, providing three of the nine histidine residues that coordinate the two metal ions, while the other histidine residues are provided by the soluble domain in this enzyme.


Pssm-ID: 425713 [Multi-domain]  Cd Length: 252  Bit Score: 67.37  E-value: 1.38e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644   73 AVWAVLYYFMTGLGITAGYHRLWAHSSYSATLPLK----IFLAAVGGGAVEGSIRWWSRDHRAHHRYT-DTDKDPYSV-- 145
Cdd:pfam00487   4 ALLLALLLGLFLLGITGSLAHEASHGALFKKRRLNrwlnDLLGRLAGLPLGISYSAWRIAHLVHHRYTnGPDKDPDTApl 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644  146 ---RKGLLYSHFGWMIMKQN------PKRIGRTDISDLNDDPVVVWQHRNYIKTVIFMGMVFPCLVTGLGWGDWFGGFIY 216
Cdd:pfam00487  84 asrFRGLLRYLLRWLLGLLVlawllaLVLPLWLRRLARRKRPIKSRRRRWRLIAWLLLLAAWLGLWLGFLGLGGLLLLLW 163

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 154294644  217 AGILRIFFVQQATFcVNSLAH----WLGDQPFDDRNSPRDHVITALVTLGEGYHNFHHEFPsdyrnAIEWHQYDPTKW 290
Cdd:pfam00487 164 LLPLLVFGFLLALI-FNYLEHyggdWGERPVETTRSIRSPNWWLNLLTGNLNYHIEHHLFP-----GVPWYRLPKLHR 235
DesA COG3239
Fatty acid desaturase [Lipid transport and metabolism];
46-285 6.48e-09

Fatty acid desaturase [Lipid transport and metabolism];


Pssm-ID: 442471 [Multi-domain]  Cd Length: 319  Bit Score: 57.05  E-value: 6.48e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644  46 WLNVTLIVGVPIYGLIMAYWTPLQWktAVWAVLYYFMTGLGITAgyHRLwAHSSYSATLPLKIFLAAVGGGAVEGSIRWW 125
Cdd:COG3239   34 LLKLALTLALLAALWLLLSWSWLAL--LAALLLGLALAGLFSLG--HDA-GHGSLFRSRWLNDLLGRLLGLPLGTPYDAW 108

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 126 SRDHRAHHRYT-DTDKDPYSV------RKGLLYSHF--GWMIMKQNPKRIGRTDISDLNDDPVVVWQHRNYIKTVIFMGM 196
Cdd:COG3239  109 RRSHNRHHAYTnDPGKDPDIGygvqawRPLYLFQHLlrFFLLGLGGLYWLLALDFLPLRGRLELKERRLEALLLLLFLAA 188

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 154294644 197 VFPCLVtGLGWGDWFGGFIYAGILRIFFvqqaTFCVNSLAHWLGDQPFDDRNSP-------RDHVITALVTLGEGYHNFH 269
Cdd:COG3239  189 LLALLL-ALGWWAVLLFWLLPLLVAGLL----LGLRFYLEHRGEDTGDGEYRDQllgsrniRGGRLLRWLFGNLNYHIEH 263

                        250
                 ....*....|....*.
gi 154294644 270 HEFPSdyrnaIEWHQY 285
Cdd:COG3239  264 HLFPS-----IPWYRL 274
PLN02252 PLN02252
nitrate reductase [NADPH]
 361-417 1.15e-04

nitrate reductase [NADPH]


Pssm-ID: 215141 [Multi-domain]  Cd Length: 888  Bit Score: 44.67  E-value: 1.15e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 154294644 361 VAGVIHDVTDFVKEHPGGKALITSGIGKDATAIFNGgvyNHSNAAHNLLSTMRVGVL 417
Cdd:PLN02252 538 VHGHVYDCTRFLKDHPGGADSILINAGTDCTEEFDA---IHSDKAKKMLEDYRIGEL 591
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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