NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|24584065|ref|NP_723799|]
View 

uncharacterized protein Dmel_CG31730 [Drosophila melanogaster]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11418877)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 57-141 6.20e-13

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 60.82  E-value: 6.20e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065  57 GYIFGQYqvkRNQDPYGHVAALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNLFMRISNRAAYQLYTSLGYAHRQTFL 136
Cdd:COG0456   1 GFALLGL---VDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERP 77


                ....*
gi 24584065 137 DYYPD 141
Cdd:COG0456  78 NYYGD 82
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 57-141 6.20e-13

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 60.82  E-value: 6.20e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065  57 GYIFGQYqvkRNQDPYGHVAALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNLFMRISNRAAYQLYTSLGYAHRQTFL 136
Cdd:COG0456   1 GFALLGL---VDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERP 77


                ....*
gi 24584065 137 DYYPD 141
Cdd:COG0456  78 NYYGD 82
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 52-129 9.17e-10

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 53.29  E-value: 9.17e-10
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 24584065    52 DGRPMGYIfgQYQVKRNQDPYGHVAALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNLFMRISNRAAYQLYTSLGY 129
Cdd:pfam00583  41 DGELVGFA--SLSIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLAAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 52-111 8.92e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 44.19  E-value: 8.92e-07
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065  52 DGRPMGYIFgqYQVKRNQDPYGHVAALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNL 111
Cdd:cd04301   7 DGEIVGFAS--LSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64
PRK03624 PRK03624
putative acetyltransferase; Provisional
 52-131 9.96e-06

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 42.99  E-value: 9.96e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065   52 DGRPMGYIFGQYQVKRnqdpyGHVAALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNLFMRISNRAAYQLYTSLGYAH 131
Cdd:PRK03624  53 GGEVVGTVMGGYDGHR-----GWAYYLAVHPDFRGRGIGRALVARLEKKLIARGCPKINLQVREDNDAVLGFYEALGYEE 127
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 57-141 6.20e-13

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 60.82  E-value: 6.20e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065  57 GYIFGQYqvkRNQDPYGHVAALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNLFMRISNRAAYQLYTSLGYAHRQTFL 136
Cdd:COG0456   1 GFALLGL---VDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERP 77


                ....*
gi 24584065 137 DYYPD 141
Cdd:COG0456  78 NYYGD 82
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 52-142 1.76e-10

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 55.44  E-value: 1.76e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065  52 DGRPMGYifgqYQVKRNQDPYGHVAALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNLFMRISNRAAYQLYTSLGYAH 131
Cdd:COG0454  42 KGEPIGF----AGLRRLDDKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERLGFKE 117

                        90
                ....*....|.
gi 24584065 132 RQTFLDYYPDE 142
Cdd:COG0454 118 IERYVAYVGGE 128
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 52-129 9.17e-10

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 53.29  E-value: 9.17e-10
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 24584065    52 DGRPMGYIfgQYQVKRNQDPYGHVAALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNLFMRISNRAAYQLYTSLGY 129
Cdd:pfam00583  41 DGELVGFA--SLSIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLAAIALYEKLGF 116
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
4-142 3.03e-09

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 52.40  E-value: 3.03e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065   4 FREMRFDDLFKINSLVFDALTEVYsLTFFVKHFLEFP--GLSQIAIApgpDGRPMGYIFGQYQVKRNQDPYGHVAALTVS 81
Cdd:COG3153   1 IRPATPEDAEAIAALLRAAFGPGR-EAELVDRLREDPaaGLSLVAED---DGEIVGHVALSPVDIDGEGPALLLGPLAVD 76

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 24584065  82 PEYRRLGLATALMDFFFMVSDLKGASYVNLFmriSNRAAYQLYTSLGYAHRQTF-LDYYPDE 142
Cdd:COG3153  77 PEYRGQGIGRALMRAALEAARERGARAVVLL---GDPSLLPFYERFGFRPAGELgLTLGPDE 135
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
52-131 4.90e-08

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 49.61  E-value: 4.90e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065  52 DGRPMGYI-FGQYqvkRNQDPYGHVA--ALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNLFMRISNRAAYQLYTSLG 128
Cdd:COG1247  60 DGEVVGFAsLGPF---RPRPAYRGTAeeSIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLG 136


                ...
gi 24584065 129 YAH 131
Cdd:COG1247 137 FEE 139
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
64-143 7.06e-08

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 47.60  E-value: 7.06e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065  64 QVKRNQDPYGHVAALTVSPEYRRLGLATALMDffFMVSDLK--GASYVNLFMRISNRAAYQLYTSLGYAHRQTFLDYYPD 141
Cdd:COG3393   7 GVRAESPGVAEISGVYTHPEYRGRGLASALVA--ALAREALarGARTPFLYVDADNPAARRLYERLGFRPVGEYATVLFR 84


                ..
gi 24584065 142 EP 143
Cdd:COG3393  85 KP 86
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 52-111 8.92e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 44.19  E-value: 8.92e-07
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065  52 DGRPMGYIFgqYQVKRNQDPYGHVAALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNL 111
Cdd:cd04301   7 DGEIVGFAS--LSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 52-129 1.31e-06

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 43.98  E-value: 1.31e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 24584065    52 DGRPMGYIFGQYQVKRNQDPYGHVAaltVSPEYRRLGLATALMDFffmVSDLKGASYVNLFMRISNRAAYQLYTSLGY 129
Cdd:pfam13508  11 DGKIVGFAALLPLDDEGALAELRLA---VHPEYRGQGIGRALLEA---AEAAAKEGGIKLLELETTNRAAAFYEKLGF 82
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 52-131 1.71e-06

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 44.98  E-value: 1.71e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065  52 DGRPMGYIfgqyQVKRNQDPYGHVAALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNLFmriSNRAAYQLYTSLGYAH 131
Cdd:COG1246  36 DGEIVGCA----ALHPLDEDLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLL---TTSAAIHFYEKLGFEE 108
PRK03624 PRK03624
putative acetyltransferase; Provisional
 52-131 9.96e-06

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 42.99  E-value: 9.96e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065   52 DGRPMGYIFGQYQVKRnqdpyGHVAALTVSPEYRRLGLATALMDFFFMVSDLKGASYVNLFMRISNRAAYQLYTSLGYAH 131
Cdd:PRK03624  53 GGEVVGTVMGGYDGHR-----GWAYYLAVHPDFRGRGIGRALVARLEKKLIARGCPKINLQVREDNDAVLGFYEALGYEE 127
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 46-142 1.82e-04

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 39.98  E-value: 1.82e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24584065  46 AIAPGPDGRPMGYI-FgqyqvkRNQDPYGHVA--ALTVSPEYRRLGLAT----ALMDFFFmvsDLKGASYVNLFMRISNR 118
Cdd:COG1670  64 AIEDKEDGELIGVVgL------YDIDRANRSAeiGYWLAPAYWGKGYATealrALLDYAF---EELGLHRVEAEVDPDNT 134

                        90       100
                ....*....|....*....|....
gi 24584065 119 AAYQLYTSLGYAHRQTFLDYYPDE 142
Cdd:COG1670 135 ASIRVLEKLGFRLEGTLRDALVID 158
rimI PRK09491
ribosomal-protein-alanine N-acetyltransferase; Provisional
 78-140 6.04e-04

ribosomal-protein-alanine N-acetyltransferase; Provisional


Pssm-ID: 181904 [Multi-domain]  Cd Length: 146  Bit Score: 37.99  E-value: 6.04e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 24584065   78 LTVSPEYRRLGLATALMDFffMVSDLKGASYVNLFM--RISNRAAYQLYTSLGYAHRQTFLDYYP 140
Cdd:PRK09491  69 IAVDPDYQRQGLGRALLEH--LIDELEKRGVATLWLevRASNAAAIALYESLGFNEVTIRRNYYP 131
Eis COG4552
Predicted acetyltransferase [General function prediction only];
73-98 2.34e-03

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 37.19  E-value: 2.34e-03
                        10        20
                ....*....|....*....|....*.
gi 24584065  73 GHVAALTVSPEYRRLGLATALMDFFF 98
Cdd:COG4552  73 AGITGVAVAPEHRRRGVARALLREAL 98
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 52-96 5.35e-03

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 34.94  E-value: 5.35e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 24584065    52 DGRPMGYIfgqyQVKRNqdpyGHVAALTVSPEYRRLGLATALMDF 96
Cdd:pfam13673  39 GGQIVGVI----ALRDR----GHISLLFVDPDYQGQGIGKALLEA 75
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH