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Conserved domains on  [gi|18401334|ref|NP_564490|]
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ChaC-like family protein [Arabidopsis thaliana]

Protein Classification

gamma-glutamylcyclotransferase family protein( domain architecture ID 366289)

gamma-glutamylcyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl

CATH:  3.10.490.10
Gene Ontology:  GO:0016746
PubMed:  18515354|20110353
SCOP:  4002485

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GGCT_like super family cl22886
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 3-178 3.23e-67

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


The actual alignment was detected with superfamily member pfam04752:

Pssm-ID: 451440  Cd Length: 176  Bit Score: 203.39  E-value: 3.23e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334     3 MWVFGYGSLIWKTGFPFDESLPGFIKGYRRVFHQGSTDHRGTPDFPGRTVTLEAAHEEVCCGVAYKITKEEdKRDALLHL 82
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDEEGRTWGVAYRIPAET-AEEVLEYL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334    83 EVREKQ--YDQKEYLDFFTDSNASEPAVAgvMVYIASPDkksNNNYLGPAPLEDIAKQIVKAKGPSGPNRDYLFNLEEAL 160
Cdd:pfam04752  80 DVREQIngYTTHEVPFYPDQDTDGRPVNA--LVYVATPK---NPQYLGDDPLEQIAKQIATARGPSGPNAEYLFELAQAL 154

                         170       180
                  ....*....|....*....|
gi 18401334   161 AQLGF--KDKHVTDLANQVR 178
Cdd:pfam04752 155 RKLGPgvQDDHLFELEKLVK 174
 
Name Accession Description Interval E-value
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 3-178 3.23e-67

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


Pssm-ID: 398428  Cd Length: 176  Bit Score: 203.39  E-value: 3.23e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334     3 MWVFGYGSLIWKTGFPFDESLPGFIKGYRRVFHQGSTDHRGTPDFPGRTVTLEAAHEEVCCGVAYKITKEEdKRDALLHL 82
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDEEGRTWGVAYRIPAET-AEEVLEYL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334    83 EVREKQ--YDQKEYLDFFTDSNASEPAVAgvMVYIASPDkksNNNYLGPAPLEDIAKQIVKAKGPSGPNRDYLFNLEEAL 160
Cdd:pfam04752  80 DVREQIngYTTHEVPFYPDQDTDGRPVNA--LVYVATPK---NPQYLGDDPLEQIAKQIATARGPSGPNAEYLFELAQAL 154

                         170       180
                  ....*....|....*....|
gi 18401334   161 AQLGF--KDKHVTDLANQVR 178
Cdd:pfam04752 155 RKLGPgvQDDHLFELEKLVK 174
ChaC2 COG3703
Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and ...
 1-185 4.76e-64

Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and metabolism];


Pssm-ID: 442917  Cd Length: 190  Bit Score: 195.81  E-value: 4.76e-64
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334   1 MAMWVFGYGSLIWKTGFPFDESLPGFIKGYRRVFHQGSTDHRGTPDFPGRTVTLEAAHEevCCGVAYKITkEEDKRDALL 80
Cdd:COG3703  14 GDLWVFGYGSLIWNPGFEFVERRPARLHGWHRSFCLRSTVHRGTPERPGLVLGLDRGGS--CRGVAFRVP-PEDAEAVLA 90

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334  81 HLEVREKQ---YDQKEyLDFFTDSNASEPAVagvmVYIASPDkksNNNYLGPAPLEDIAKQIVKAKGPSGPNRDYLFNLE 157
Cdd:COG3703  91 YLWRREMVtgvYLPRW-LPVRLADGRRVEAL----AFVADRD---HPQYAGGLSLEEQARIIAQAVGPSGSNADYLLNTA 162

                       170       180
                ....*....|....*....|....*...
gi 18401334 158 EALAQLGFKDKHVTDLANQVRHILSESE 185
Cdd:COG3703 163 EHLRELGIRDPHLEALARLVRALLAAAT 190
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 5-116 1.64e-11

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 58.13  E-value: 1.64e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334   5 VFGYGSLIWKTGF-----PFDESLPGFIKGYRRVFHQGStdhrgtpDFPGrtvtLEAAHEEVCCGVAYKITKEEdkRDAL 79
Cdd:cd06661   1 LFVYGTLMDGEVLharlgRALFLGPATLKGYRLVFGGGS-------GYPG----LVPGPGARVWGELYEVDPED--LARL 67

                        90       100       110
                ....*....|....*....|....*....|....*..
gi 18401334  80 LHLEVREKQYDqKEYLDFFTDSNASEPAvagvMVYIA 116
Cdd:cd06661  68 DAFEGVPGGYR-REEVEVELEDGEGVEA----WVYVA 99
 
Name Accession Description Interval E-value
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 3-178 3.23e-67

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


Pssm-ID: 398428  Cd Length: 176  Bit Score: 203.39  E-value: 3.23e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334     3 MWVFGYGSLIWKTGFPFDESLPGFIKGYRRVFHQGSTDHRGTPDFPGRTVTLEAAHEEVCCGVAYKITKEEdKRDALLHL 82
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDEEGRTWGVAYRIPAET-AEEVLEYL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334    83 EVREKQ--YDQKEYLDFFTDSNASEPAVAgvMVYIASPDkksNNNYLGPAPLEDIAKQIVKAKGPSGPNRDYLFNLEEAL 160
Cdd:pfam04752  80 DVREQIngYTTHEVPFYPDQDTDGRPVNA--LVYVATPK---NPQYLGDDPLEQIAKQIATARGPSGPNAEYLFELAQAL 154

                         170       180
                  ....*....|....*....|
gi 18401334   161 AQLGF--KDKHVTDLANQVR 178
Cdd:pfam04752 155 RKLGPgvQDDHLFELEKLVK 174
ChaC2 COG3703
Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and ...
 1-185 4.76e-64

Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and metabolism];


Pssm-ID: 442917  Cd Length: 190  Bit Score: 195.81  E-value: 4.76e-64
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334   1 MAMWVFGYGSLIWKTGFPFDESLPGFIKGYRRVFHQGSTDHRGTPDFPGRTVTLEAAHEevCCGVAYKITkEEDKRDALL 80
Cdd:COG3703  14 GDLWVFGYGSLIWNPGFEFVERRPARLHGWHRSFCLRSTVHRGTPERPGLVLGLDRGGS--CRGVAFRVP-PEDAEAVLA 90

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334  81 HLEVREKQ---YDQKEyLDFFTDSNASEPAVagvmVYIASPDkksNNNYLGPAPLEDIAKQIVKAKGPSGPNRDYLFNLE 157
Cdd:COG3703  91 YLWRREMVtgvYLPRW-LPVRLADGRRVEAL----AFVADRD---HPQYAGGLSLEEQARIIAQAVGPSGSNADYLLNTA 162

                       170       180
                ....*....|....*....|....*...
gi 18401334 158 EALAQLGFKDKHVTDLANQVRHILSESE 185
Cdd:COG3703 163 EHLRELGIRDPHLEALARLVRALLAAAT 190
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 5-116 1.64e-11

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 58.13  E-value: 1.64e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18401334   5 VFGYGSLIWKTGF-----PFDESLPGFIKGYRRVFHQGStdhrgtpDFPGrtvtLEAAHEEVCCGVAYKITKEEdkRDAL 79
Cdd:cd06661   1 LFVYGTLMDGEVLharlgRALFLGPATLKGYRLVFGGGS-------GYPG----LVPGPGARVWGELYEVDPED--LARL 67

                        90       100       110
                ....*....|....*....|....*....|....*..
gi 18401334  80 LHLEVREKQYDqKEYLDFFTDSNASEPAvagvMVYIA 116
Cdd:cd06661  68 DAFEGVPGGYR-REEVEVELEDGEGVEA----WVYVA 99
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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