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Conserved domains on  [gi|227330590|ref|NP_080470|]
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E3 UFM1-protein ligase 1 [Mus musculus]

Protein Classification

E3 UFM1-protein ligase 1( domain architecture ID 10560867)

E3 UFM1-protein ligase 1 acts as E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to lysine residues on target proteins, and which plays a key role in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
E3_UFM1_ligase pfam09743
E3 UFM1-protein ligase 1; The ubiquitin fold modifier 1 (Ufm1) is the most recently discovered ...
 7-284 2.30e-156

E3 UFM1-protein ligase 1; The ubiquitin fold modifier 1 (Ufm1) is the most recently discovered ubiquitin-like modifier whose conjugation (ufmylation) system is conserved in multicellular organizms. Ufm1 is known to covalently attach with cellular protein(s) via a specific E1-activating enzyme (Uba5), an E2-conjugating enzyme (Ufc1), and a E3-ligating enzyme.


:

Pssm-ID: 462874  Cd Length: 272  Bit Score: 455.85  E-value: 2.30e-156
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590    7 EIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVI 86
Cdd:pfam09743   1 EILELAADFQRAQLSSSSQRLSERNCIEIVNKLIELKLLDLIHTTDGKEYITPEQLEKEIKDELLAHGGRVNLVDLAKIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590   87 NVDLTHIESRVSDIIKSEKHVQMVLGQLIDENYLDQLSEEVNDKLQESGQVTVSELCKAYDLPGDFLTQALTQRLGRIIN 166
Cdd:pfam09743  81 NVDLDHIERQAQEIVKEDPELMLVNGELITKSYLDSIAEEINEKLQECGQITIAELAKQYDLPSDFLLSLLEKRLGKIIK 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590  167 GHLDldnRGVIFTEAFVARHKARIRGLFSAITRPTPVNSLVSKYGFQEQLLYSVLEDLVSTGRLRGTVVGGRqdkAVFVP 246
Cdd:pfam09743 161 GVLD---GGVLYTEAYVARHKAKVRGALRAITRPTPLSALLKRYGLPEKLFYSLLEELIKEGRIPGSLIGGR---AVYIP 234

                         250       260       270
                  ....*....|....*....|....*....|....*...
gi 227330590  247 DIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYI 284
Cdd:pfam09743 235 TIYSKTQSEWVDSFYKQNGYIEYDTLQRLGISDPKSYL 272
 
Name Accession Description Interval E-value
E3_UFM1_ligase pfam09743
E3 UFM1-protein ligase 1; The ubiquitin fold modifier 1 (Ufm1) is the most recently discovered ...
 7-284 2.30e-156

E3 UFM1-protein ligase 1; The ubiquitin fold modifier 1 (Ufm1) is the most recently discovered ubiquitin-like modifier whose conjugation (ufmylation) system is conserved in multicellular organizms. Ufm1 is known to covalently attach with cellular protein(s) via a specific E1-activating enzyme (Uba5), an E2-conjugating enzyme (Ufc1), and a E3-ligating enzyme.


Pssm-ID: 462874  Cd Length: 272  Bit Score: 455.85  E-value: 2.30e-156
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590    7 EIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVI 86
Cdd:pfam09743   1 EILELAADFQRAQLSSSSQRLSERNCIEIVNKLIELKLLDLIHTTDGKEYITPEQLEKEIKDELLAHGGRVNLVDLAKIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590   87 NVDLTHIESRVSDIIKSEKHVQMVLGQLIDENYLDQLSEEVNDKLQESGQVTVSELCKAYDLPGDFLTQALTQRLGRIIN 166
Cdd:pfam09743  81 NVDLDHIERQAQEIVKEDPELMLVNGELITKSYLDSIAEEINEKLQECGQITIAELAKQYDLPSDFLLSLLEKRLGKIIK 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590  167 GHLDldnRGVIFTEAFVARHKARIRGLFSAITRPTPVNSLVSKYGFQEQLLYSVLEDLVSTGRLRGTVVGGRqdkAVFVP 246
Cdd:pfam09743 161 GVLD---GGVLYTEAYVARHKAKVRGALRAITRPTPLSALLKRYGLPEKLFYSLLEELIKEGRIPGSLIGGR---AVYIP 234

                         250       260       270
                  ....*....|....*....|....*....|....*...
gi 227330590  247 DIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYI 284
Cdd:pfam09743 235 TIYSKTQSEWVDSFYKQNGYIEYDTLQRLGISDPKSYL 272
PLN03083 PLN03083
E3 UFM1-protein ligase 1 homolog; Provisional
 6-788 3.65e-105

E3 UFM1-protein ligase 1 homolog; Provisional


Pssm-ID: 215565  Cd Length: 803  Bit Score: 341.42  E-value: 3.65e-105
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590   6 EEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQL--EVVHTLDGKEYITPAQISKEMRDELHvRGGRVNIVDLQ 83
Cdd:PLN03083   3 AELLELQRQFEFAQQAKSSVRLSERNVVELVQKLQELGIIdfDLLHTVSGKEYITQDQLRNEIEAEIK-KLGRVSLVDLA 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590  84 QVINVDLTHIESRVSDIIKSEKHVQMVLGQLIDENYLDQLSEEVNDKLQESGQVTVSELCKAYDLPGDFLTQALTQRLGR 163
Cdd:PLN03083  82 DTIGVDLYHVERQAQQVVSDDPGLMLVQGEIISQSYWDSIAEEINERLQECSQIALAELARQLQVGSELVTSMLEPRLGT 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 164 IINGHLDldnRGVIFTEAFVARHKARIRGLFSAITRPTPVNSLvskYGFQEQLL----------------YSVLEDLVST 227
Cdd:PLN03083 162 IVKARLE---GGQLYTPAYVARVTAMVRGAARGITVPTNLSSL---WNSLQQLLremdgaggvtvegsffQSIFNGLVKE 235

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 228 GRLRGTVVGGrqdkAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYIKKRYKNTqlLFLKATCVGQGLVD 307
Cdd:PLN03083 236 EEVLGSLRAG----THWTPAVFAHAQKESVDAFFSQNSYITYDTLQKLGISQPKQFLQSRYPDG--IALDAVFVHSSMIE 309

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 308 QVEASVEEAISSGTWVDISPLLPSSLSVEDAAMLLqqVMRPFGKLA----SAIVFSDTVVVSEKFItdcTGLFsERMHQK 383
Cdd:PLN03083 310 MLDSAAEDAIEGGSWIDSLSVLPSSFGSQDAGKLL--LLCPSVQSAlkdsKALILGESCVLSNGFI---KGIY-DQIEKE 383

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 384 AEKEMKNNPVHLITEEDLKQISILESVNTSKKDKKDERR---KKATEGSGSVRGGGGGNAREYKIKKTKKKGRKDEDSDD 460
Cdd:PLN03083 384 MDAFSIQASSAGLIGSSEKSLGSNESSPAASNSDKGSKKkkgKSTSTKGGTAESIPDDEEDAPKKGKKNQKKGRDKSSKV 463

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 461 ESQSSHGGKK-----KPDITFMFQDEIedcLRKHIQDAPEEFISE-----------LAEYLIKPLNKMYLEVVRSVFmss 524
Cdd:PLN03083 464 PSDSKAGGKKesvksQEDNNNIPPEEW---VMKKILEWVPDLEEDgtedpgsilkhLADHLRPMLINSLKERRKALF--- 537

                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 525 tSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYFADDTQTA--LTKHLLKTVCTDITNLMFNFLASDFLMA----VEE 598
Cdd:PLN03083 538 -TENAERRRRLLDNLQKKIDESFLNMQLYEKALDLFEDDQSTSvvLHRHLLRTTAAEIADTLLHTLDIHNKLKngteVED 616

                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 599 PAA-----ITSDIRKKILSKLTEETKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALCE 673
Cdd:PLN03083 617 SKTqdtvlLSSAERTALAKNLPGSLSSKALALVEALEGKRVDAFMSTLRDLAEESGLRLKKLDKKLERTLLHSYRKDLTS 696

                        730       740       750       760       770       780       790       800
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 674 QLKVTEDPALILHLTAVLLFQLSTHSMLHAPGRCVPQIIAFLHSKIPEDQHTLLVKYQGLVVKQLVSQNKKTGQGEDPSS 753
Cdd:PLN03083 697 QVSTESDPVALLPKVVSLLYVQVHNKALQAPGRAISAAISRLKDKLPDSAYKTLTDYHSATVTLLALMSAATGEEHDCSS 776

                        810       820       830
                 ....*....|....*....|....*....|....*
gi 227330590 754 DELDKEqhdvtnatRKELQELSLSIKDLVLKSRKS 788
Cdd:PLN03083 777 DRILTK--------RELLESLMPPLKSLVLGDSQP 803
 
Name Accession Description Interval E-value
E3_UFM1_ligase pfam09743
E3 UFM1-protein ligase 1; The ubiquitin fold modifier 1 (Ufm1) is the most recently discovered ...
 7-284 2.30e-156

E3 UFM1-protein ligase 1; The ubiquitin fold modifier 1 (Ufm1) is the most recently discovered ubiquitin-like modifier whose conjugation (ufmylation) system is conserved in multicellular organizms. Ufm1 is known to covalently attach with cellular protein(s) via a specific E1-activating enzyme (Uba5), an E2-conjugating enzyme (Ufc1), and a E3-ligating enzyme.


Pssm-ID: 462874  Cd Length: 272  Bit Score: 455.85  E-value: 2.30e-156
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590    7 EIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVI 86
Cdd:pfam09743   1 EILELAADFQRAQLSSSSQRLSERNCIEIVNKLIELKLLDLIHTTDGKEYITPEQLEKEIKDELLAHGGRVNLVDLAKIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590   87 NVDLTHIESRVSDIIKSEKHVQMVLGQLIDENYLDQLSEEVNDKLQESGQVTVSELCKAYDLPGDFLTQALTQRLGRIIN 166
Cdd:pfam09743  81 NVDLDHIERQAQEIVKEDPELMLVNGELITKSYLDSIAEEINEKLQECGQITIAELAKQYDLPSDFLLSLLEKRLGKIIK 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590  167 GHLDldnRGVIFTEAFVARHKARIRGLFSAITRPTPVNSLVSKYGFQEQLLYSVLEDLVSTGRLRGTVVGGRqdkAVFVP 246
Cdd:pfam09743 161 GVLD---GGVLYTEAYVARHKAKVRGALRAITRPTPLSALLKRYGLPEKLFYSLLEELIKEGRIPGSLIGGR---AVYIP 234

                         250       260       270
                  ....*....|....*....|....*....|....*...
gi 227330590  247 DIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYI 284
Cdd:pfam09743 235 TIYSKTQSEWVDSFYKQNGYIEYDTLQRLGISDPKSYL 272
PLN03083 PLN03083
E3 UFM1-protein ligase 1 homolog; Provisional
 6-788 3.65e-105

E3 UFM1-protein ligase 1 homolog; Provisional


Pssm-ID: 215565  Cd Length: 803  Bit Score: 341.42  E-value: 3.65e-105
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590   6 EEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQL--EVVHTLDGKEYITPAQISKEMRDELHvRGGRVNIVDLQ 83
Cdd:PLN03083   3 AELLELQRQFEFAQQAKSSVRLSERNVVELVQKLQELGIIdfDLLHTVSGKEYITQDQLRNEIEAEIK-KLGRVSLVDLA 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590  84 QVINVDLTHIESRVSDIIKSEKHVQMVLGQLIDENYLDQLSEEVNDKLQESGQVTVSELCKAYDLPGDFLTQALTQRLGR 163
Cdd:PLN03083  82 DTIGVDLYHVERQAQQVVSDDPGLMLVQGEIISQSYWDSIAEEINERLQECSQIALAELARQLQVGSELVTSMLEPRLGT 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 164 IINGHLDldnRGVIFTEAFVARHKARIRGLFSAITRPTPVNSLvskYGFQEQLL----------------YSVLEDLVST 227
Cdd:PLN03083 162 IVKARLE---GGQLYTPAYVARVTAMVRGAARGITVPTNLSSL---WNSLQQLLremdgaggvtvegsffQSIFNGLVKE 235

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 228 GRLRGTVVGGrqdkAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYIKKRYKNTqlLFLKATCVGQGLVD 307
Cdd:PLN03083 236 EEVLGSLRAG----THWTPAVFAHAQKESVDAFFSQNSYITYDTLQKLGISQPKQFLQSRYPDG--IALDAVFVHSSMIE 309

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 308 QVEASVEEAISSGTWVDISPLLPSSLSVEDAAMLLqqVMRPFGKLA----SAIVFSDTVVVSEKFItdcTGLFsERMHQK 383
Cdd:PLN03083 310 MLDSAAEDAIEGGSWIDSLSVLPSSFGSQDAGKLL--LLCPSVQSAlkdsKALILGESCVLSNGFI---KGIY-DQIEKE 383

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 384 AEKEMKNNPVHLITEEDLKQISILESVNTSKKDKKDERR---KKATEGSGSVRGGGGGNAREYKIKKTKKKGRKDEDSDD 460
Cdd:PLN03083 384 MDAFSIQASSAGLIGSSEKSLGSNESSPAASNSDKGSKKkkgKSTSTKGGTAESIPDDEEDAPKKGKKNQKKGRDKSSKV 463

                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 461 ESQSSHGGKK-----KPDITFMFQDEIedcLRKHIQDAPEEFISE-----------LAEYLIKPLNKMYLEVVRSVFmss 524
Cdd:PLN03083 464 PSDSKAGGKKesvksQEDNNNIPPEEW---VMKKILEWVPDLEEDgtedpgsilkhLADHLRPMLINSLKERRKALF--- 537

                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 525 tSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYFADDTQTA--LTKHLLKTVCTDITNLMFNFLASDFLMA----VEE 598
Cdd:PLN03083 538 -TENAERRRRLLDNLQKKIDESFLNMQLYEKALDLFEDDQSTSvvLHRHLLRTTAAEIADTLLHTLDIHNKLKngteVED 616

                        650       660       670       680       690       700       710       720
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 599 PAA-----ITSDIRKKILSKLTEETKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALCE 673
Cdd:PLN03083 617 SKTqdtvlLSSAERTALAKNLPGSLSSKALALVEALEGKRVDAFMSTLRDLAEESGLRLKKLDKKLERTLLHSYRKDLTS 696

                        730       740       750       760       770       780       790       800
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 227330590 674 QLKVTEDPALILHLTAVLLFQLSTHSMLHAPGRCVPQIIAFLHSKIPEDQHTLLVKYQGLVVKQLVSQNKKTGQGEDPSS 753
Cdd:PLN03083 697 QVSTESDPVALLPKVVSLLYVQVHNKALQAPGRAISAAISRLKDKLPDSAYKTLTDYHSATVTLLALMSAATGEEHDCSS 776

                        810       820       830
                 ....*....|....*....|....*....|....*
gi 227330590 754 DELDKEqhdvtnatRKELQELSLSIKDLVLKSRKS 788
Cdd:PLN03083 777 DRILTK--------RELLESLMPPLKSLVLGDSQP 803
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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