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Conserved domains on  [gi|110431354|ref|NP_073606|]
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CREB-regulated transcription coactivator 3 isoform a [Homo sapiens]

Protein Classification

CREB-regulated transcription coactivator( domain architecture ID 10583816)

CREB-regulated transcription coactivator enhances the activity of CRE-depended transcription via a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB (cAMP Response Element-Binding)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TORC_M pfam12885
Transducer of regulated CREB activity middle domain; This family includes the region between ...
 159-319 4.38e-70

Transducer of regulated CREB activity middle domain; This family includes the region between the N and C terminus of TORC proteins. TORC (Transducer of regulated CREB activity) is a protein family of coactivators that enhances the activity of CRE-depended transcription via a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB (cAMP Response Element-Binding). Although the C- and N- terminal domains of these proteins have been well characterized, no functional role has been assigned to the central region, yet.


:

Pssm-ID: 463739  Cd Length: 154  Bit Score: 223.47  E-value: 4.38e-70
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354  159 RTNSDSALHTSALSTKPQDPYGGGGQSAWPAPYmgfcdgennGHGEVASFPGPLKEENLLNVPKPLPKQLWETKEIQSLS 238
Cdd:pfam12885   1 RTNSDSALHTSAMNPNPQDPFTGGSQELQPAPR---------GFGRVLLLTVPGTEENLLEVDKPLPKQLWDAKKVQSLS 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354  239 GRPRSCDVGGGNAFPHNGQNLGLSPFLGTLNTGGSLPDLTNLHYSTPLPASLDTTDHHFGSMSVGNSVNNIPAAMTHLGI 318
Cdd:pfam12885  72 SRPKSCEVPGINIFPSPDQNATLSLFPAALNTGGSLPDLTNLHFPSPLPTPLDPEDTAFPSLSGGNSTGNLAAAMTHLGI 151


                  .
gi 110431354  319 R 319
Cdd:pfam12885 152 S 152
TORC_C pfam12886
Transducer of regulated CREB activity, C terminus; This family includes the C terminal region ...
 545-619 2.12e-33

Transducer of regulated CREB activity, C terminus; This family includes the C terminal region of TORC proteins. TORC (Transducer of regulated CREB activity) is a protein family of coactivators that enhances the activity of CRE-depended transcription via a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB (cAMP Response Element-Binding). The C terminus region is negatively charged, resembling the transcription activation domains. When this domain, from all three human TORC proteins, was expressed as fusion proteins with the DNA-binding domain of GAL4 (GAL4-BD), and tested for induction of a minimal promoter linked to GAL4-binding sites (UAS-GAL4), UAS-GAL4 was potently induced by GAL4-BD fusions containing the C-terminal portion of all three human TORCs.


:

Pssm-ID: 463740  Cd Length: 75  Bit Score: 122.12  E-value: 2.12e-33
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 110431354  545 PNTILPEDSSTSLFKDLNSALAGLPEVSLNVDTPFPLEEELQIEPLSLDGLNMLSDSSMGLLDPSVEETFRADRL 619
Cdd:pfam12886   1 PNIILTGDSSPGFSKDLSSALAGVPEVSFDADDLFPLDDELGIEPLDLDGLQMLSDPDMVLPDPATEDTFRSDRL 75
TORC_N pfam12884
Transducer of regulated CREB activity, N terminus; This family includes the N terminal region ...
 11-64 1.49e-18

Transducer of regulated CREB activity, N terminus; This family includes the N terminal region of TORC proteins. TORC (Transducer of regulated CREB activity) is a protein family of coactivators that enhances the activity of CRE-depended transcription via a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB (cAMP Response Element-Binding). The proteins display a highly conserved predicted N-terminal coiled-coil domain and an invariant sequence matching a protein kinase A (PKA) phosphorylation consensus sequence (RKXS). The coiled-coil structure interacts with the bZIP domain of CREB. This interaction may occur via ionic bonds because it is disrupted under high-salt conditions. In addition to CREB-binding, the N-terminal region plays a role in the tetramer formation of TORCs, but the physiological function of the multimeric complex has not been clarified yet.


:

Pssm-ID: 463738  Cd Length: 63  Bit Score: 79.80  E-value: 1.49e-18
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 110431354   11 NPRKFSEKIALHTQRQAEETRAFEQLMTDLTLSRV-----QFQKLQQLRLT----QYHGGSLP 64
Cdd:pfam12884   1 NPRKFSEKIALHKQKQAEETAAFERIMREVSATTRdptqsSNQKQQQLRLGllgpQYRGGSLP 63
PHA03378 super family cl33729
EBNA-3B; Provisional
 346-537 3.35e-03

EBNA-3B; Provisional


The actual alignment was detected with superfamily member PHA03378:

Pssm-ID: 223065 [Multi-domain]  Cd Length: 991  Bit Score: 40.44  E-value: 3.35e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354 346 LNNHPQTSVPNASaLHPSLRLFSLSNPSLSTTNLS---GPSRRRQPPVSPLTLSPGPEAHQGFSRQLSSTSPLAPYPTSQ 422
Cdd:PHA03378 672 IPYQPSPTGANTM-LPIQWAPGTMQPPPRAPTPMRppaAPPGRAQRPAAATGRARPPAAAPGRARPPAAAPGRARPPAAA 750

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354 423 MVSSDRSQLSFLPTEAQAQVSPPPPYPAPQELTQPLLQQPRAPEAPAQQPQAAS-----SLPQSDFQLLPAQGSSLTNFF 497
Cdd:PHA03378 751 PGRARPPAAAPGRARPPAAAPGAPTPQPPPQAPPAPQQRPRGAPTPQPPPQAGPtsmqlMPRAAPGQQGPTKQILRQLLT 830

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 110431354 498 PDVGFDQQSMRPGPAFPQQVPLVQQGSRELQDSFHLRPSP 537
Cdd:PHA03378 831 GGVKRGRPSLKKPAALERQAAAGPTPSPGSGTSDKIVQAP 870
 
Name Accession Description Interval E-value
TORC_M pfam12885
Transducer of regulated CREB activity middle domain; This family includes the region between ...
 159-319 4.38e-70

Transducer of regulated CREB activity middle domain; This family includes the region between the N and C terminus of TORC proteins. TORC (Transducer of regulated CREB activity) is a protein family of coactivators that enhances the activity of CRE-depended transcription via a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB (cAMP Response Element-Binding). Although the C- and N- terminal domains of these proteins have been well characterized, no functional role has been assigned to the central region, yet.


Pssm-ID: 463739  Cd Length: 154  Bit Score: 223.47  E-value: 4.38e-70
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354  159 RTNSDSALHTSALSTKPQDPYGGGGQSAWPAPYmgfcdgennGHGEVASFPGPLKEENLLNVPKPLPKQLWETKEIQSLS 238
Cdd:pfam12885   1 RTNSDSALHTSAMNPNPQDPFTGGSQELQPAPR---------GFGRVLLLTVPGTEENLLEVDKPLPKQLWDAKKVQSLS 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354  239 GRPRSCDVGGGNAFPHNGQNLGLSPFLGTLNTGGSLPDLTNLHYSTPLPASLDTTDHHFGSMSVGNSVNNIPAAMTHLGI 318
Cdd:pfam12885  72 SRPKSCEVPGINIFPSPDQNATLSLFPAALNTGGSLPDLTNLHFPSPLPTPLDPEDTAFPSLSGGNSTGNLAAAMTHLGI 151


                  .
gi 110431354  319 R 319
Cdd:pfam12885 152 S 152
TORC_C pfam12886
Transducer of regulated CREB activity, C terminus; This family includes the C terminal region ...
 545-619 2.12e-33

Transducer of regulated CREB activity, C terminus; This family includes the C terminal region of TORC proteins. TORC (Transducer of regulated CREB activity) is a protein family of coactivators that enhances the activity of CRE-depended transcription via a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB (cAMP Response Element-Binding). The C terminus region is negatively charged, resembling the transcription activation domains. When this domain, from all three human TORC proteins, was expressed as fusion proteins with the DNA-binding domain of GAL4 (GAL4-BD), and tested for induction of a minimal promoter linked to GAL4-binding sites (UAS-GAL4), UAS-GAL4 was potently induced by GAL4-BD fusions containing the C-terminal portion of all three human TORCs.


Pssm-ID: 463740  Cd Length: 75  Bit Score: 122.12  E-value: 2.12e-33
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 110431354  545 PNTILPEDSSTSLFKDLNSALAGLPEVSLNVDTPFPLEEELQIEPLSLDGLNMLSDSSMGLLDPSVEETFRADRL 619
Cdd:pfam12886   1 PNIILTGDSSPGFSKDLSSALAGVPEVSFDADDLFPLDDELGIEPLDLDGLQMLSDPDMVLPDPATEDTFRSDRL 75
TORC_N pfam12884
Transducer of regulated CREB activity, N terminus; This family includes the N terminal region ...
 11-64 1.49e-18

Transducer of regulated CREB activity, N terminus; This family includes the N terminal region of TORC proteins. TORC (Transducer of regulated CREB activity) is a protein family of coactivators that enhances the activity of CRE-depended transcription via a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB (cAMP Response Element-Binding). The proteins display a highly conserved predicted N-terminal coiled-coil domain and an invariant sequence matching a protein kinase A (PKA) phosphorylation consensus sequence (RKXS). The coiled-coil structure interacts with the bZIP domain of CREB. This interaction may occur via ionic bonds because it is disrupted under high-salt conditions. In addition to CREB-binding, the N-terminal region plays a role in the tetramer formation of TORCs, but the physiological function of the multimeric complex has not been clarified yet.


Pssm-ID: 463738  Cd Length: 63  Bit Score: 79.80  E-value: 1.49e-18
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 110431354   11 NPRKFSEKIALHTQRQAEETRAFEQLMTDLTLSRV-----QFQKLQQLRLT----QYHGGSLP 64
Cdd:pfam12884   1 NPRKFSEKIALHKQKQAEETAAFERIMREVSATTRdptqsSNQKQQQLRLGllgpQYRGGSLP 63
PHA03378 PHA03378
EBNA-3B; Provisional
 346-537 3.35e-03

EBNA-3B; Provisional


Pssm-ID: 223065 [Multi-domain]  Cd Length: 991  Bit Score: 40.44  E-value: 3.35e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354 346 LNNHPQTSVPNASaLHPSLRLFSLSNPSLSTTNLS---GPSRRRQPPVSPLTLSPGPEAHQGFSRQLSSTSPLAPYPTSQ 422
Cdd:PHA03378 672 IPYQPSPTGANTM-LPIQWAPGTMQPPPRAPTPMRppaAPPGRAQRPAAATGRARPPAAAPGRARPPAAAPGRARPPAAA 750

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354 423 MVSSDRSQLSFLPTEAQAQVSPPPPYPAPQELTQPLLQQPRAPEAPAQQPQAAS-----SLPQSDFQLLPAQGSSLTNFF 497
Cdd:PHA03378 751 PGRARPPAAAPGRARPPAAAPGAPTPQPPPQAPPAPQQRPRGAPTPQPPPQAGPtsmqlMPRAAPGQQGPTKQILRQLLT 830

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 110431354 498 PDVGFDQQSMRPGPAFPQQVPLVQQGSRELQDSFHLRPSP 537
Cdd:PHA03378 831 GGVKRGRPSLKKPAALERQAAAGPTPSPGSGTSDKIVQAP 870
 
Name Accession Description Interval E-value
TORC_M pfam12885
Transducer of regulated CREB activity middle domain; This family includes the region between ...
 159-319 4.38e-70

Transducer of regulated CREB activity middle domain; This family includes the region between the N and C terminus of TORC proteins. TORC (Transducer of regulated CREB activity) is a protein family of coactivators that enhances the activity of CRE-depended transcription via a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB (cAMP Response Element-Binding). Although the C- and N- terminal domains of these proteins have been well characterized, no functional role has been assigned to the central region, yet.


Pssm-ID: 463739  Cd Length: 154  Bit Score: 223.47  E-value: 4.38e-70
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354  159 RTNSDSALHTSALSTKPQDPYGGGGQSAWPAPYmgfcdgennGHGEVASFPGPLKEENLLNVPKPLPKQLWETKEIQSLS 238
Cdd:pfam12885   1 RTNSDSALHTSAMNPNPQDPFTGGSQELQPAPR---------GFGRVLLLTVPGTEENLLEVDKPLPKQLWDAKKVQSLS 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354  239 GRPRSCDVGGGNAFPHNGQNLGLSPFLGTLNTGGSLPDLTNLHYSTPLPASLDTTDHHFGSMSVGNSVNNIPAAMTHLGI 318
Cdd:pfam12885  72 SRPKSCEVPGINIFPSPDQNATLSLFPAALNTGGSLPDLTNLHFPSPLPTPLDPEDTAFPSLSGGNSTGNLAAAMTHLGI 151


                  .
gi 110431354  319 R 319
Cdd:pfam12885 152 S 152
TORC_C pfam12886
Transducer of regulated CREB activity, C terminus; This family includes the C terminal region ...
 545-619 2.12e-33

Transducer of regulated CREB activity, C terminus; This family includes the C terminal region of TORC proteins. TORC (Transducer of regulated CREB activity) is a protein family of coactivators that enhances the activity of CRE-depended transcription via a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB (cAMP Response Element-Binding). The C terminus region is negatively charged, resembling the transcription activation domains. When this domain, from all three human TORC proteins, was expressed as fusion proteins with the DNA-binding domain of GAL4 (GAL4-BD), and tested for induction of a minimal promoter linked to GAL4-binding sites (UAS-GAL4), UAS-GAL4 was potently induced by GAL4-BD fusions containing the C-terminal portion of all three human TORCs.


Pssm-ID: 463740  Cd Length: 75  Bit Score: 122.12  E-value: 2.12e-33
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 110431354  545 PNTILPEDSSTSLFKDLNSALAGLPEVSLNVDTPFPLEEELQIEPLSLDGLNMLSDSSMGLLDPSVEETFRADRL 619
Cdd:pfam12886   1 PNIILTGDSSPGFSKDLSSALAGVPEVSFDADDLFPLDDELGIEPLDLDGLQMLSDPDMVLPDPATEDTFRSDRL 75
TORC_N pfam12884
Transducer of regulated CREB activity, N terminus; This family includes the N terminal region ...
 11-64 1.49e-18

Transducer of regulated CREB activity, N terminus; This family includes the N terminal region of TORC proteins. TORC (Transducer of regulated CREB activity) is a protein family of coactivators that enhances the activity of CRE-depended transcription via a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB (cAMP Response Element-Binding). The proteins display a highly conserved predicted N-terminal coiled-coil domain and an invariant sequence matching a protein kinase A (PKA) phosphorylation consensus sequence (RKXS). The coiled-coil structure interacts with the bZIP domain of CREB. This interaction may occur via ionic bonds because it is disrupted under high-salt conditions. In addition to CREB-binding, the N-terminal region plays a role in the tetramer formation of TORCs, but the physiological function of the multimeric complex has not been clarified yet.


Pssm-ID: 463738  Cd Length: 63  Bit Score: 79.80  E-value: 1.49e-18
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 110431354   11 NPRKFSEKIALHTQRQAEETRAFEQLMTDLTLSRV-----QFQKLQQLRLT----QYHGGSLP 64
Cdd:pfam12884   1 NPRKFSEKIALHKQKQAEETAAFERIMREVSATTRdptqsSNQKQQQLRLGllgpQYRGGSLP 63
PHA03378 PHA03378
EBNA-3B; Provisional
 346-537 3.35e-03

EBNA-3B; Provisional


Pssm-ID: 223065 [Multi-domain]  Cd Length: 991  Bit Score: 40.44  E-value: 3.35e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354 346 LNNHPQTSVPNASaLHPSLRLFSLSNPSLSTTNLS---GPSRRRQPPVSPLTLSPGPEAHQGFSRQLSSTSPLAPYPTSQ 422
Cdd:PHA03378 672 IPYQPSPTGANTM-LPIQWAPGTMQPPPRAPTPMRppaAPPGRAQRPAAATGRARPPAAAPGRARPPAAAPGRARPPAAA 750

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 110431354 423 MVSSDRSQLSFLPTEAQAQVSPPPPYPAPQELTQPLLQQPRAPEAPAQQPQAAS-----SLPQSDFQLLPAQGSSLTNFF 497
Cdd:PHA03378 751 PGRARPPAAAPGRARPPAAAPGAPTPQPPPQAPPAPQQRPRGAPTPQPPPQAGPtsmqlMPRAAPGQQGPTKQILRQLLT 830

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 110431354 498 PDVGFDQQSMRPGPAFPQQVPLVQQGSRELQDSFHLRPSP 537
Cdd:PHA03378 831 GGVKRGRPSLKKPAALERQAAAGPTPSPGSGTSDKIVQAP 870
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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