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Conserved domains on  [gi|1406928158|ref|NP_001351450|]
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pre-mRNA-splicing factor SLU7 isoform b [Homo sapiens]

Protein Classification

pre-mRNA-splicing factor SLU7( domain architecture ID 10569510)

pre-mRNA-splicing factor SLU7 is required for pre-mRNA splicing as component of the spliceosome

Gene Ontology:  GO:0000398|GO:0030628
PubMed:  15181151|7664739

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Slu7 pfam11708
Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4 ...
 161-434 6.35e-145

Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4/U6, and U5) and proteins, catalyzes the excision of introns from pre-mRNAs in two successive trans-esterification reactions. Step 2 depends upon integral spliceosome constituents such as U5 snRNA and Prp8 and non-spliceosomal proteins Prp16, Slu7, Prp18, and Prp22. ATP hydrolysis by the DEAH-box enzyme Prp16 promotes a conformational change in the spliceosome that leads to protection of the 3'ss from targeted RNase H cleavage. This change, which probably reflects binding of the 3'ss PyAG in the catalytic centre of the spliceosome, requires the ordered recruitment of Slu7, Prp18, and Prp22 to the spliceosome. There is a close functional relationship between Prp8, Prp18, and Slu7, and Prp18 interacts with Slu7, so that together they recruit Prp22 to the spliceosome. Most members of the family carry a zinc-finger of the CCHC-type upstream of this domain.


:

Pssm-ID: 463326  Cd Length: 258  Bit Score: 418.98  E-value: 6.35e-145
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1406928158 161 FDYDGKRDRWNGYNPEEHMKIVEEYAKVDLAKRTLKAQKLQEELAsgklveqanspkhqwgEEEPNSQMEKDHNSEDEDE 240
Cdd:pfam11708   1 LGYDAKRDRWNGYDPSEYKKVVEEYEKLEEARKKLKEEELDKKEK----------------DDEGDDDDSEEEDNDDEDE 64

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1406928158 241 DKYADDIDMPGQNFDSKRRITVRNLRIREDIAKYLRNLDPNSAYYDPKTRAMRENPYANAGKNPDEVSYAGDNFVRYTGD 320
Cdd:pfam11708  65 DKYADEADMGGQKVDSKQRTTVRNLRIREDTAKYLLNLDLNSAYYDPKTRSMRDNPLPNVDPSEDKALFAGDNFVRASGD 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1406928158 321 TISMAQTQLFAWEAYDKGSEVHLQADPTKLELLYKSFKVKKEDFKEQQKESILEKYGGQEHLDAPPAELLLAQTEDYVEY 400
Cdd:pfam11708 145 AAEFEKLQRFAWEAQEKGDDVHLQANPTQAELLYKEFKEKKEKLKEKKKKSILEKYGGEEHLEAPPKELLLAQTERYVEY 224

                         250       260       270
                  ....*....|....*....|....*....|....
gi 1406928158 401 SRHGTVIKGQERAVACSKYEEDVKIHNHTHIWGS 434
Cdd:pfam11708 225 DRSGRVIKGQEKAKAKSKYEEDVYINNHTSVWGS 258
 
Name Accession Description Interval E-value
Slu7 pfam11708
Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4 ...
 161-434 6.35e-145

Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4/U6, and U5) and proteins, catalyzes the excision of introns from pre-mRNAs in two successive trans-esterification reactions. Step 2 depends upon integral spliceosome constituents such as U5 snRNA and Prp8 and non-spliceosomal proteins Prp16, Slu7, Prp18, and Prp22. ATP hydrolysis by the DEAH-box enzyme Prp16 promotes a conformational change in the spliceosome that leads to protection of the 3'ss from targeted RNase H cleavage. This change, which probably reflects binding of the 3'ss PyAG in the catalytic centre of the spliceosome, requires the ordered recruitment of Slu7, Prp18, and Prp22 to the spliceosome. There is a close functional relationship between Prp8, Prp18, and Slu7, and Prp18 interacts with Slu7, so that together they recruit Prp22 to the spliceosome. Most members of the family carry a zinc-finger of the CCHC-type upstream of this domain.


Pssm-ID: 463326  Cd Length: 258  Bit Score: 418.98  E-value: 6.35e-145
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1406928158 161 FDYDGKRDRWNGYNPEEHMKIVEEYAKVDLAKRTLKAQKLQEELAsgklveqanspkhqwgEEEPNSQMEKDHNSEDEDE 240
Cdd:pfam11708   1 LGYDAKRDRWNGYDPSEYKKVVEEYEKLEEARKKLKEEELDKKEK----------------DDEGDDDDSEEEDNDDEDE 64

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1406928158 241 DKYADDIDMPGQNFDSKRRITVRNLRIREDIAKYLRNLDPNSAYYDPKTRAMRENPYANAGKNPDEVSYAGDNFVRYTGD 320
Cdd:pfam11708  65 DKYADEADMGGQKVDSKQRTTVRNLRIREDTAKYLLNLDLNSAYYDPKTRSMRDNPLPNVDPSEDKALFAGDNFVRASGD 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1406928158 321 TISMAQTQLFAWEAYDKGSEVHLQADPTKLELLYKSFKVKKEDFKEQQKESILEKYGGQEHLDAPPAELLLAQTEDYVEY 400
Cdd:pfam11708 145 AAEFEKLQRFAWEAQEKGDDVHLQANPTQAELLYKEFKEKKEKLKEKKKKSILEKYGGEEHLEAPPKELLLAQTERYVEY 224

                         250       260       270
                  ....*....|....*....|....*....|....
gi 1406928158 401 SRHGTVIKGQERAVACSKYEEDVKIHNHTHIWGS 434
Cdd:pfam11708 225 DRSGRVIKGQEKAKAKSKYEEDVYINNHTSVWGS 258
 
Name Accession Description Interval E-value
Slu7 pfam11708
Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4 ...
 161-434 6.35e-145

Pre-mRNA splicing Prp18-interacting factor; The spliceosome, an assembly of snRNAs (U1, U2, U4/U6, and U5) and proteins, catalyzes the excision of introns from pre-mRNAs in two successive trans-esterification reactions. Step 2 depends upon integral spliceosome constituents such as U5 snRNA and Prp8 and non-spliceosomal proteins Prp16, Slu7, Prp18, and Prp22. ATP hydrolysis by the DEAH-box enzyme Prp16 promotes a conformational change in the spliceosome that leads to protection of the 3'ss from targeted RNase H cleavage. This change, which probably reflects binding of the 3'ss PyAG in the catalytic centre of the spliceosome, requires the ordered recruitment of Slu7, Prp18, and Prp22 to the spliceosome. There is a close functional relationship between Prp8, Prp18, and Slu7, and Prp18 interacts with Slu7, so that together they recruit Prp22 to the spliceosome. Most members of the family carry a zinc-finger of the CCHC-type upstream of this domain.


Pssm-ID: 463326  Cd Length: 258  Bit Score: 418.98  E-value: 6.35e-145
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1406928158 161 FDYDGKRDRWNGYNPEEHMKIVEEYAKVDLAKRTLKAQKLQEELAsgklveqanspkhqwgEEEPNSQMEKDHNSEDEDE 240
Cdd:pfam11708   1 LGYDAKRDRWNGYDPSEYKKVVEEYEKLEEARKKLKEEELDKKEK----------------DDEGDDDDSEEEDNDDEDE 64

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1406928158 241 DKYADDIDMPGQNFDSKRRITVRNLRIREDIAKYLRNLDPNSAYYDPKTRAMRENPYANAGKNPDEVSYAGDNFVRYTGD 320
Cdd:pfam11708  65 DKYADEADMGGQKVDSKQRTTVRNLRIREDTAKYLLNLDLNSAYYDPKTRSMRDNPLPNVDPSEDKALFAGDNFVRASGD 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1406928158 321 TISMAQTQLFAWEAYDKGSEVHLQADPTKLELLYKSFKVKKEDFKEQQKESILEKYGGQEHLDAPPAELLLAQTEDYVEY 400
Cdd:pfam11708 145 AAEFEKLQRFAWEAQEKGDDVHLQANPTQAELLYKEFKEKKEKLKEKKKKSILEKYGGEEHLEAPPKELLLAQTERYVEY 224

                         250       260       270
                  ....*....|....*....|....*....|....
gi 1406928158 401 SRHGTVIKGQERAVACSKYEEDVKIHNHTHIWGS 434
Cdd:pfam11708 225 DRSGRVIKGQEKAKAKSKYEEDVYINNHTSVWGS 258
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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