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Conserved domains on  [gi|1371542563|ref|NP_001348310|]
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malignant T-cell-amplified sequence 1 isoform 3 [Mus musculus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PUA_MCTS-1-like cd21155
PUA RNA-binding domain of malignant T cell-amplified sequence 1 and related proteins; The ...
 29-124 1.75e-73

PUA RNA-binding domain of malignant T cell-amplified sequence 1 and related proteins; The RNA-binding PUA (PseudoUridine synthase and Archaeosine transglycosylase) domain was detected in a number of proteins involved in RNA metabolism. Members of this eukaryotic family, labelled MCT-1 (malignant T cell-amplified sequence 1) or MCTS-1 (multiple copies T-cell lymphoma-1), contain a single PUA domain. They may play roles in the regulation of the cell cycle; human MCT-1 has been characterized for its oncogenic potential. MCT-1/MCTS1 expression is a new poor-prognosis marker in patients with aggressive breast cancers, and thus the MCT-1 pathway is a novel and promising therapeutic target for triple-negative breast cancer (TNBC).


:

Pssm-ID: 409297  Cd Length: 97  Bit Score: 213.50  E-value: 1.75e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563  29 PTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTSPGAKLYPA-AVDTIVAIMAEGKQHALCVGVMKMSAEDIEKV 107
Cdd:cd21155     1 PTLRLLHKYPFMLPKVQVDKGAIKFVLSGANIMCPGLTSPGGKLPDDvEKGTVVAIMAEGKEHALAIGITKMSSEDIKKV 80

                          90
                  ....*....|....*..
gi 1371542563 108 NKGIGIENIHYLNDGLW 124
Cdd:cd21155    81 NKGIGIENIHYLGDGLW 97
Pre-PUA super family cl38935
Pre-PUA-like domain; This Pre-PUA-like domain is found in a wide variety of proteins including ...
 5-38 1.11e-12

Pre-PUA-like domain; This Pre-PUA-like domain is found in a wide variety of proteins including the eukaryotic translation initiation factor 2D, where it is found at the N-terminus.


The actual alignment was detected with superfamily member pfam17832:

Pssm-ID: 436077  Cd Length: 86  Bit Score: 59.12  E-value: 1.11e-12
                          10        20        30
                  ....*....|....*....|....*....|....
gi 1371542563   5 HEHIEILTVNGELLFFRQREGPFYPTLRLLHKYP 38
Cdd:pfam17832  53 TEHVSLYSVDGEPLFFQVRDGPLYPTLYLLWKYP 86
 
Name Accession Description Interval E-value
PUA_MCTS-1-like cd21155
PUA RNA-binding domain of malignant T cell-amplified sequence 1 and related proteins; The ...
 29-124 1.75e-73

PUA RNA-binding domain of malignant T cell-amplified sequence 1 and related proteins; The RNA-binding PUA (PseudoUridine synthase and Archaeosine transglycosylase) domain was detected in a number of proteins involved in RNA metabolism. Members of this eukaryotic family, labelled MCT-1 (malignant T cell-amplified sequence 1) or MCTS-1 (multiple copies T-cell lymphoma-1), contain a single PUA domain. They may play roles in the regulation of the cell cycle; human MCT-1 has been characterized for its oncogenic potential. MCT-1/MCTS1 expression is a new poor-prognosis marker in patients with aggressive breast cancers, and thus the MCT-1 pathway is a novel and promising therapeutic target for triple-negative breast cancer (TNBC).


Pssm-ID: 409297  Cd Length: 97  Bit Score: 213.50  E-value: 1.75e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563  29 PTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTSPGAKLYPA-AVDTIVAIMAEGKQHALCVGVMKMSAEDIEKV 107
Cdd:cd21155     1 PTLRLLHKYPFMLPKVQVDKGAIKFVLSGANIMCPGLTSPGGKLPDDvEKGTVVAIMAEGKEHALAIGITKMSSEDIKKV 80

                          90
                  ....*....|....*..
gi 1371542563 108 NKGIGIENIHYLNDGLW 124
Cdd:cd21155    81 NKGIGIENIHYLGDGLW 97
unchar_dom_2 TIGR00451
uncharacterized domain 2; This uncharacterized domain is found a number of enzymes and ...
 13-121 9.37e-29

uncharacterized domain 2; This uncharacterized domain is found a number of enzymes and uncharacterized proteins, often at the C-terminus. It is found in some but not all members of a family of related tRNA-guanine transglycosylases (tgt), which exchange a guanine base for some modified base without breaking the phosphodiester backbone of the tRNA. It is also found in rRNA pseudouridine synthase, another enzyme of RNA base modification not otherwise homologous to tgt. It is found, again at the C-terminus, in two putative glutamate 5-kinases. It is also found in a family of small, uncharacterized archaeal proteins consisting mostly of this domain.


Pssm-ID: 129543 [Multi-domain]  Cd Length: 107  Bit Score: 100.97  E-value: 9.37e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563  13 VNGELLFFrQREGPFYPTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTSPGAKLypaAVDTIVAIMAEGKQHAL 92
Cdd:TIGR00451   3 VDGEPLYF-IYDDKVIPSLKGALKLMEDKKIVVVDNGAVKFLKNGADVMRPGIVDADEDI---KEGDDVVVVDENKDRPL 78

                          90       100
                  ....*....|....*....|....*....
gi 1371542563  93 CVGVMKMSAEDIEKVNKGIGIENIHYLND 121
Cdd:TIGR00451  79 AVGIALMSGEEMKEMDKGKAVKNIHHIGD 107
Tma20 COG2016
Predicted ribosome-associated RNA-binding protein Tma20, contains PUA domain [Translation, ...
 5-124 9.91e-29

Predicted ribosome-associated RNA-binding protein Tma20, contains PUA domain [Translation, ribosomal structure and biogenesis];


Pssm-ID: 441619 [Multi-domain]  Cd Length: 154  Bit Score: 102.17  E-value: 9.91e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563   5 HEHIEILTVNGELLFFRQREGPFyPTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTSPGAKLYPaavDTIVAIM 84
Cdd:COG2016    38 TDDFEIYLVDGEPLLFKVDDEPF-PTLRGLLKYPPEKPVVTVDMGAVKFVSNGADVMRPGIVEADGEIKE---GDIVVIV 113

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1371542563  85 AEGKQHALCVGVMKMSAEDIEKVNKGIGIENIHYLNDGLW 124
Cdd:COG2016   114 EEKHGKPLAVGRALVDGEEMVEGKKGKAVKNLHHVGDKLW 153
PRK14560 PRK14560
putative RNA-binding protein; Provisional
 9-127 1.31e-26

putative RNA-binding protein; Provisional


Pssm-ID: 237757 [Multi-domain]  Cd Length: 160  Bit Score: 96.84  E-value: 1.31e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563   9 EILTVNGELLFFRQREGPFyPTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTS--PGAKlypaaVDTIVAIMAE 86
Cdd:PRK14560   45 EIYLVDGEPLFFKVDDELF-PTLRGALKLKPEKRRVVVDAGAVKFVSNGADVMAPGIVEadEDIK-----EGDIVFVVEE 118

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1371542563  87 GKQHALCVGVMKMSAEDIEKVNKGIGIENIHYLNDGLWHMK 127
Cdd:PRK14560  119 THGKPLAVGRALMDGDEMVEEKKGKAVKNIHHVGDEIWEFE 159
PUA pfam01472
PUA domain; The PUA domain named after Pseudouridine synthase and Archaeosine transglycosylase, ...
 42-119 1.67e-18

PUA domain; The PUA domain named after Pseudouridine synthase and Archaeosine transglycosylase, was detected in archaeal and eukaryotic pseudouridine synthases, archaeal archaeosine synthases, a family of predicted ATPases that may be involved in RNA modification, a family of predicted archaeal and bacterial rRNA methylases. Additionally, the PUA domain was detected in a family of eukaryotic proteins that also contain a domain homologous to the translation initiation factor eIF1/SUI1; these proteins may comprise a novel type of translation factors. Unexpectedly, the PUA domain was detected also in bacterial and yeast glutamate kinases; this is compatible with the demonstrated role of these enzymes in the regulation of the expression of other genes. It is predicted that the PUA domain is an RNA binding domain.


Pssm-ID: 426278 [Multi-domain]  Cd Length: 74  Bit Score: 73.67  E-value: 1.67e-18
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1371542563  42 PHQQVDKGAIKFVLSGANIMCPGLTSPGAKLypaAVDTIVAIMAEgKQHALCVGVMKMSAEDIEKVNKGIGIENIHYL 119
Cdd:pfam01472   1 GRVVVDDGAVKAILNGASLLAPGVVRVDGDF---RKGDEVVVVTE-KGELVAVGLANYSSEELAKIEGGKAVKVRRVL 74
Pre-PUA pfam17832
Pre-PUA-like domain; This Pre-PUA-like domain is found in a wide variety of proteins including ...
 5-38 1.11e-12

Pre-PUA-like domain; This Pre-PUA-like domain is found in a wide variety of proteins including the eukaryotic translation initiation factor 2D, where it is found at the N-terminus.


Pssm-ID: 436077  Cd Length: 86  Bit Score: 59.12  E-value: 1.11e-12
                          10        20        30
                  ....*....|....*....|....*....|....
gi 1371542563   5 HEHIEILTVNGELLFFRQREGPFYPTLRLLHKYP 38
Cdd:pfam17832  53 TEHVSLYSVDGEPLFFQVRDGPLYPTLYLLWKYP 86
PUA smart00359
Putative RNA-binding Domain in PseudoUridine synthase and Archaeosine transglycosylase;
46-119 2.17e-11

Putative RNA-binding Domain in PseudoUridine synthase and Archaeosine transglycosylase;


Pssm-ID: 214635 [Multi-domain]  Cd Length: 76  Bit Score: 55.73  E-value: 2.17e-11
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1371542563   46 VDKGAIKFVLSGANIMCPGLTSPGAKLYPaavDTIVAIMAEgKQHALCVGVMKMSAEDIEKVN-KGIGIENIHYL 119
Cdd:smart00359   5 VDDGAEKAILNGASLLAPGVVRVDGDIKE---GDVVVIVDE-KGEPLGIGLANMSSEEIARIKgKGLAVKVRRAV 75
MCT1_N cd11609
N-terminal domain of multiple copies T cell malignancies 1 and related proteins; This ...
 3-29 4.57e-11

N-terminal domain of multiple copies T cell malignancies 1 and related proteins; This N-terminal domain of MCT-1 (multiple copies T cell malignancies 1), also known as MCTS-1 (malignant T cell-amplified sequence 1), co-occurs with a PUA domain. MCT-1, together with DENR (density regulated protein), has been shown to have similar function as eIF2D translation initiation factor (also known as ligatin), which is involved in the recruitment and delivery of aminoacyl-tRNAs to the P-site of the eukaryotic ribosome in a GTP-independent manner.


Pssm-ID: 211422  Cd Length: 77  Bit Score: 54.85  E-value: 4.57e-11
                          10        20
                  ....*....|....*....|....*..
gi 1371542563   3 KNHEHIEILTVNGELLFFRQREGPFYP 29
Cdd:cd11609    51 KCHDHIELLVVNGEPLFFQHRDGPYIP 77
 
Name Accession Description Interval E-value
PUA_MCTS-1-like cd21155
PUA RNA-binding domain of malignant T cell-amplified sequence 1 and related proteins; The ...
 29-124 1.75e-73

PUA RNA-binding domain of malignant T cell-amplified sequence 1 and related proteins; The RNA-binding PUA (PseudoUridine synthase and Archaeosine transglycosylase) domain was detected in a number of proteins involved in RNA metabolism. Members of this eukaryotic family, labelled MCT-1 (malignant T cell-amplified sequence 1) or MCTS-1 (multiple copies T-cell lymphoma-1), contain a single PUA domain. They may play roles in the regulation of the cell cycle; human MCT-1 has been characterized for its oncogenic potential. MCT-1/MCTS1 expression is a new poor-prognosis marker in patients with aggressive breast cancers, and thus the MCT-1 pathway is a novel and promising therapeutic target for triple-negative breast cancer (TNBC).


Pssm-ID: 409297  Cd Length: 97  Bit Score: 213.50  E-value: 1.75e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563  29 PTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTSPGAKLYPA-AVDTIVAIMAEGKQHALCVGVMKMSAEDIEKV 107
Cdd:cd21155     1 PTLRLLHKYPFMLPKVQVDKGAIKFVLSGANIMCPGLTSPGGKLPDDvEKGTVVAIMAEGKEHALAIGITKMSSEDIKKV 80

                          90
                  ....*....|....*..
gi 1371542563 108 NKGIGIENIHYLNDGLW 124
Cdd:cd21155    81 NKGIGIENIHYLGDGLW 97
unchar_dom_2 TIGR00451
uncharacterized domain 2; This uncharacterized domain is found a number of enzymes and ...
 13-121 9.37e-29

uncharacterized domain 2; This uncharacterized domain is found a number of enzymes and uncharacterized proteins, often at the C-terminus. It is found in some but not all members of a family of related tRNA-guanine transglycosylases (tgt), which exchange a guanine base for some modified base without breaking the phosphodiester backbone of the tRNA. It is also found in rRNA pseudouridine synthase, another enzyme of RNA base modification not otherwise homologous to tgt. It is found, again at the C-terminus, in two putative glutamate 5-kinases. It is also found in a family of small, uncharacterized archaeal proteins consisting mostly of this domain.


Pssm-ID: 129543 [Multi-domain]  Cd Length: 107  Bit Score: 100.97  E-value: 9.37e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563  13 VNGELLFFrQREGPFYPTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTSPGAKLypaAVDTIVAIMAEGKQHAL 92
Cdd:TIGR00451   3 VDGEPLYF-IYDDKVIPSLKGALKLMEDKKIVVVDNGAVKFLKNGADVMRPGIVDADEDI---KEGDDVVVVDENKDRPL 78

                          90       100
                  ....*....|....*....|....*....
gi 1371542563  93 CVGVMKMSAEDIEKVNKGIGIENIHYLND 121
Cdd:TIGR00451  79 AVGIALMSGEEMKEMDKGKAVKNIHHIGD 107
Tma20 COG2016
Predicted ribosome-associated RNA-binding protein Tma20, contains PUA domain [Translation, ...
 5-124 9.91e-29

Predicted ribosome-associated RNA-binding protein Tma20, contains PUA domain [Translation, ribosomal structure and biogenesis];


Pssm-ID: 441619 [Multi-domain]  Cd Length: 154  Bit Score: 102.17  E-value: 9.91e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563   5 HEHIEILTVNGELLFFRQREGPFyPTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTSPGAKLYPaavDTIVAIM 84
Cdd:COG2016    38 TDDFEIYLVDGEPLLFKVDDEPF-PTLRGLLKYPPEKPVVTVDMGAVKFVSNGADVMRPGIVEADGEIKE---GDIVVIV 113

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1371542563  85 AEGKQHALCVGVMKMSAEDIEKVNKGIGIENIHYLNDGLW 124
Cdd:COG2016   114 EEKHGKPLAVGRALVDGEEMVEGKKGKAVKNLHHVGDKLW 153
arCOG00985 TIGR03684
arCOG04150 universal archaeal PUA-domain protein; This universal archaeal protein contains a ...
 9-124 7.19e-27

arCOG04150 universal archaeal PUA-domain protein; This universal archaeal protein contains a domain possibly associated with RNA binding (pfam01472, TIGR00451).


Pssm-ID: 274723 [Multi-domain]  Cd Length: 150  Bit Score: 97.29  E-value: 7.19e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563   9 EILTVNGELLFFrQREGPFYPTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTSpgaklypaAVDTI-----VAI 83
Cdd:TIGR03684  38 EIYLVDGKPLLF-EVDGRLFPTLYGLLELNPDKNVVVVDEGAVKFIINGADIMAPGIVE--------ADPSIkegdiVFV 108

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1371542563  84 MAEGKQHALCVGVMKMSAEDIEKVNKGIGIENIHYLNDGLW 124
Cdd:TIGR03684 109 VDETHGKPLAVGIALMDAEEMVEEKKGKAVKNIHHVGDKIW 149
PRK14560 PRK14560
putative RNA-binding protein; Provisional
 9-127 1.31e-26

putative RNA-binding protein; Provisional


Pssm-ID: 237757 [Multi-domain]  Cd Length: 160  Bit Score: 96.84  E-value: 1.31e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563   9 EILTVNGELLFFRQREGPFyPTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTS--PGAKlypaaVDTIVAIMAE 86
Cdd:PRK14560   45 EIYLVDGEPLFFKVDDELF-PTLRGALKLKPEKRRVVVDAGAVKFVSNGADVMAPGIVEadEDIK-----EGDIVFVVEE 118

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1371542563  87 GKQHALCVGVMKMSAEDIEKVNKGIGIENIHYLNDGLWHMK 127
Cdd:PRK14560  119 THGKPLAVGRALMDGDEMVEEKKGKAVKNIHHVGDEIWEFE 159
PUA_MJ1432-like cd21154
PUA RNA-binding domain of MJ1432, TA1423, PH0734, and similar proteins; The RNA-binding PUA ...
 41-126 3.12e-20

PUA RNA-binding domain of MJ1432, TA1423, PH0734, and similar proteins; The RNA-binding PUA (PseudoUridine synthase and Archaeosine transglycosylase) domain was detected in a number of proteins involved in RNA metabolism. Members of this mostly archaeal family have not been characterized functionally; they may bind to RNA. This family includes Pyrococcus horikoshii PH0734 where the N-terminal domain may modulate the binding target of the C-terminal PUA domain using its characteristic electropositive surface.


Pssm-ID: 409296 [Multi-domain]  Cd Length: 84  Bit Score: 78.31  E-value: 3.12e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563  41 LPHQQVDKGAIKFVLSGANIMCPGLT--SPGAKLypaavDTIVAIMAEGKQHALCVGVMKMSAEDIEKVNKGIGIENIHY 118
Cdd:cd21154     2 LPRVVVDMGAVKFVANGADVMRPGIVeaDEEIKK-----GDIVVVVDERHGKPLAVGIALMSGEEMVEMKKGKAVKNLHY 76


                  ....*...
gi 1371542563 119 LNDGLWHM 126
Cdd:cd21154    77 VGDKIWKL 84
PUA cd07953
PUA RNA binding domain; The PUA (PseudoUridine synthase and Archaeosine transglycosylase) ...
 42-118 3.75e-20

PUA RNA binding domain; The PUA (PseudoUridine synthase and Archaeosine transglycosylase) domain was detected in archaeal and eukaryotic pseudouridine synthases, archaeal archaeosine synthases, a family of predicted ATPases that may be involved in RNA modification, and a family of predicted archaeal and bacterial rRNA methylases. Additionally, the PUA domain was detected in a family of eukaryotic proteins that also contain a domain homologous to the translation initiation factor eIF1/SUI1; these proteins may comprise a novel type of translation factors. Unexpectedly, the PUA domain was also found in bacterial and yeast glutamate kinases; this is compatible with the demonstrated role of these enzymes in regulating the expression of other genes. It has been shown that the PUA domain acts as an RNA binding domain in at least some of the proteins involved in RNA metabolism.


Pssm-ID: 409289 [Multi-domain]  Cd Length: 73  Bit Score: 77.72  E-value: 3.75e-20
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1371542563  42 PHQQVDKGAIKFVLSGANIMCPGLTSPgakLYPAAVDTIVAIMAEGKqHALCVGVMKMSAEDIEKVNKGIGIENIHY 118
Cdd:cd07953     1 PVVVVDKGAEKAVLNGADLMAPGVVSA---DGDFKRGDLVRIVSEGG-RPLAIGVAEMSSDEMKEELKGIAVRVLHF 73
PUA pfam01472
PUA domain; The PUA domain named after Pseudouridine synthase and Archaeosine transglycosylase, ...
 42-119 1.67e-18

PUA domain; The PUA domain named after Pseudouridine synthase and Archaeosine transglycosylase, was detected in archaeal and eukaryotic pseudouridine synthases, archaeal archaeosine synthases, a family of predicted ATPases that may be involved in RNA modification, a family of predicted archaeal and bacterial rRNA methylases. Additionally, the PUA domain was detected in a family of eukaryotic proteins that also contain a domain homologous to the translation initiation factor eIF1/SUI1; these proteins may comprise a novel type of translation factors. Unexpectedly, the PUA domain was detected also in bacterial and yeast glutamate kinases; this is compatible with the demonstrated role of these enzymes in the regulation of the expression of other genes. It is predicted that the PUA domain is an RNA binding domain.


Pssm-ID: 426278 [Multi-domain]  Cd Length: 74  Bit Score: 73.67  E-value: 1.67e-18
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1371542563  42 PHQQVDKGAIKFVLSGANIMCPGLTSPGAKLypaAVDTIVAIMAEgKQHALCVGVMKMSAEDIEKVNKGIGIENIHYL 119
Cdd:pfam01472   1 GRVVVDDGAVKAILNGASLLAPGVVRVDGDF---RKGDEVVVVTE-KGELVAVGLANYSSEELAKIEGGKAVKVRRVL 74
Pre-PUA pfam17832
Pre-PUA-like domain; This Pre-PUA-like domain is found in a wide variety of proteins including ...
 5-38 1.11e-12

Pre-PUA-like domain; This Pre-PUA-like domain is found in a wide variety of proteins including the eukaryotic translation initiation factor 2D, where it is found at the N-terminus.


Pssm-ID: 436077  Cd Length: 86  Bit Score: 59.12  E-value: 1.11e-12
                          10        20        30
                  ....*....|....*....|....*....|....
gi 1371542563   5 HEHIEILTVNGELLFFRQREGPFYPTLRLLHKYP 38
Cdd:pfam17832  53 TEHVSLYSVDGEPLFFQVRDGPLYPTLYLLWKYP 86
PUA smart00359
Putative RNA-binding Domain in PseudoUridine synthase and Archaeosine transglycosylase;
46-119 2.17e-11

Putative RNA-binding Domain in PseudoUridine synthase and Archaeosine transglycosylase;


Pssm-ID: 214635 [Multi-domain]  Cd Length: 76  Bit Score: 55.73  E-value: 2.17e-11
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1371542563   46 VDKGAIKFVLSGANIMCPGLTSPGAKLYPaavDTIVAIMAEgKQHALCVGVMKMSAEDIEKVN-KGIGIENIHYL 119
Cdd:smart00359   5 VDDGAEKAILNGASLLAPGVVRVDGDIKE---GDVVVIVDE-KGEPLGIGLANMSSEEIARIKgKGLAVKVRRAV 75
MCT1_N cd11609
N-terminal domain of multiple copies T cell malignancies 1 and related proteins; This ...
 3-29 4.57e-11

N-terminal domain of multiple copies T cell malignancies 1 and related proteins; This N-terminal domain of MCT-1 (multiple copies T cell malignancies 1), also known as MCTS-1 (malignant T cell-amplified sequence 1), co-occurs with a PUA domain. MCT-1, together with DENR (density regulated protein), has been shown to have similar function as eIF2D translation initiation factor (also known as ligatin), which is involved in the recruitment and delivery of aminoacyl-tRNAs to the P-site of the eukaryotic ribosome in a GTP-independent manner.


Pssm-ID: 211422  Cd Length: 77  Bit Score: 54.85  E-value: 4.57e-11
                          10        20
                  ....*....|....*....|....*..
gi 1371542563   3 KNHEHIEILTVNGELLFFRQREGPFYP 29
Cdd:cd11609    51 KCHDHIELLVVNGEPLFFQHRDGPYIP 77
PUA_eIF2d-like cd21156
PUA RNA-binding domain of eukaryotic translation initiation factor 2D and similar proteins; ...
 38-120 8.16e-09

PUA RNA-binding domain of eukaryotic translation initiation factor 2D and similar proteins; The RNA-binding PUA (PseudoUridine synthase and Archaeosine transglycosylase) domain was detected in a number of proteins involved in RNA metabolism. Most members of this eukaryotic translation initiation factor 2D (eIF2d)-like family of eukaryotic proteins also contain a domain homologous to the translation initiation factor eIF1/SUI1, and a short uncharacterized N-terminal domain. eIF2D may function as a cytosolic GTP-independent initiation factor which delivers Met-tRNA (and non-initiating tRNAs) to the 40S ribosomal subunit. The family member from Drosophila melanogaster has been named ligatin, and this alias has been adopted for other family members as well, which are not homologous to the vertebrate ligatin (LGTN) that is a trafficking receptor for phosphoglycoproteins.


Pssm-ID: 409298 [Multi-domain]  Cd Length: 82  Bit Score: 49.10  E-value: 8.16e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1371542563  38 PFILPHQQVdkgaIKFVLSGANIMCPGLTSPGAKLYPAAVDTIVAIMAEGKQHALCVGVMKMSAEDIEKVN-KGIGIENI 116
Cdd:cd21156     3 PVFTTHPPV----SEKLLGGADLMLPGVIVPPPGLPPFEKGSLVAVAVLGNPAPVAVGRAAMSSEDMYASGmKGKGVEVL 78


                  ....
gi 1371542563 117 HYLN 120
Cdd:cd21156    79 HTYG 82
eIF2D_N_like cd11580
N-terminal domain of eIF2D, malignant T cell-amplified sequence 1 and related proteins; This ...
 2-29 1.10e-05

N-terminal domain of eIF2D, malignant T cell-amplified sequence 1 and related proteins; This N-terminal domain of various proteins co-occurs with a PUA domain. Members of this family are: (1) MCTS-1 (malignant T cell-amplified sequence 1) or MCT-1 (multiple copies T cell malignancies), which may play roles in the regulation of the cell cycle, (2) the eukayotic translation initiation factor 2D, and (3) an uncharacterized archaeal family.


Pssm-ID: 211421  Cd Length: 72  Bit Score: 40.80  E-value: 1.10e-05
                          10        20
                  ....*....|....*....|....*...
gi 1371542563   2 FKNHEHIEILTVNGELLFFRQREGPFYP 29
Cdd:cd11580    45 QKFDTHYEIYTVDGEPVFFELDEGEVFP 72
PRK13795 PRK13795
hypothetical protein; Provisional
 46-114 2.57e-05

hypothetical protein; Provisional


Pssm-ID: 237510 [Multi-domain]  Cd Length: 636  Bit Score: 42.29  E-value: 2.57e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1371542563  46 VDKGAIKFVLSGANIMCPGLTS--PGAKLYpaavDTIVAIMAEGKQHAlcVGVMKMSAEDIEKVNKGIGIE 114
Cdd:PRK13795  131 VDKGALEPIKNGKNVLAPGVVEadLDIKKG----DEVVVVTEDGEVVG--VGRAKMDGDDMIKRFRGRAVK 195
PUA_archaeosine_TGT cd21149
PUA RNA-binding domain of archaeosine tRNA-guanine transglycosylase; The RNA-binding PUA ...
 46-114 2.27e-03

PUA RNA-binding domain of archaeosine tRNA-guanine transglycosylase; The RNA-binding PUA (PseudoUridine synthase and Archaeosine transglycosylase) domain was detected in a number of proteins involved in RNA metabolism. Members of this archaeosine tRNA-guanine transglycosylase (TGT) family are responsible for the exchange of a guanine residue in archaeal tRNAs with a preQ0 base (7-cyano-7-deazaguanine), which constitutes the initial step in archaeosine biosynthesis. Archaeosine is a modified RNA base specific to archaea (7-formamidino-7deazaguanosine), found at position 15 in tRNAs. It has been shown that the PUA domain of archaeosine TGT is not required for its specificity for position 15.


Pssm-ID: 409291 [Multi-domain]  Cd Length: 75  Bit Score: 34.51  E-value: 2.27e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1371542563  46 VDKGAIKFVLSGANIMCPGLTSPGAKLYPAavdTIVAIMAEGKQhALCVGVMKMSAEDIEKVNKGIGIE 114
Cdd:cd21149     7 VNKESAPFVRKGGSVFAKGVVDADENIRPG---DEVLVVDEDDR-LLAVGRAVLSGKEMKEFERGVAVK 71
PRK04270 PRK04270
RNA-guided pseudouridylation complex pseudouridine synthase subunit Cbf5;
41-114 2.96e-03

RNA-guided pseudouridylation complex pseudouridine synthase subunit Cbf5;


Pssm-ID: 179806 [Multi-domain]  Cd Length: 300  Bit Score: 35.99  E-value: 2.96e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1371542563  41 LPHQQVDKGAIKFVLSGANIMCPGLtspgAKLY-PAAVDTIVAIMAEgKQHALCVGVMKMSAEDIEKVNKGIGIE 114
Cdd:PRK04270  225 LPKIIIKDSAVDAIAHGAPLYAPGI----AKLEkGIKKGDLVAVFTL-KGELVALGKALMDSDEILKAEKGIVVD 294
PUA_Cbf5 cd21148
PUA RNA-binding domain of the archaeal pseudouridine synthase component Cbf5; The RNA-binding ...
 57-111 3.25e-03

PUA RNA-binding domain of the archaeal pseudouridine synthase component Cbf5; The RNA-binding PUA (PseudoUridine synthase and Archaeosine transglycosylase) domain was detected in a number of proteins involved in RNA metabolism. Members of the archaeal and eukaryotic subfamily of pseudouridine synthases, including Cbf5 (dyskerin in humans) and similar proteins, are modules that assist in the binding and positioning (guide and/or substrate) of RNA to the pseudouridine synthase complex. Pseudouridine synthases are enzymes that are responsible for post-translational modifications of RNAs by specifically isomerizing uracil residues. In Pyrococcus furiosus H/ACA ribonucleoprotein (RNP) assembly with a single-hairpin H/ACA RNA, the lower stem and the ACA motif of the guide RNA are anchored at the PUA domain of Cbf5. In addition, the N-terminal extension of Cbf5, which is a hot spot for dyskeratosis congenita (a rare genetic form of bone marrow failure) mutation, forms an extra structural layer on the PUA domain.


Pssm-ID: 409290 [Multi-domain]  Cd Length: 75  Bit Score: 33.98  E-value: 3.25e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1371542563  57 GANIMCPGLtspgAKLYPA-AVDTIVAIMAEgKQHALCVGVMKMSAEDIEKVNKGI 111
Cdd:cd21148    18 GAKLAIPGV----LRYEDGiEKGDEVVIMTT-KGEAVALGIALMTTAEIATCDHGI 68
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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