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Conserved domains on  [gi|1063729171|ref|NP_001318659|]
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tRNA (cytidine(34)-2'-O)-methyltransferase [Arabidopsis thaliana]

Protein Classification

tRNA (cytidine(34)-2'-O)-methyltransferase( domain architecture ID 13031049)

tRNA (cytidine(34)-2'-O)-methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine to the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)

CATH:  3.40.1280.10
EC:  2.1.1.207
Gene Ontology:  GO:0003723|GO:0008175|GO:0141098|GO:1904047|GO:0030488
PubMed:  11763972|17338813|30457841|18029778

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 83-237 4.63e-83

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


:

Pssm-ID: 349967  Cd Length: 145  Bit Score: 245.05  E-value: 4.63e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  83 HVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARVKRAGLDYWPFVVVKAHSSWAEFQEYFRlqegEKRMIAF 162
Cdd:cd18094     1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALP----PGRLFLF 76

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1063729171 163 TKRGTRIHSDFSYRSGDYLLFGSETSGLPPEALSDCNHEpygggTLRIPMVETyVRCLNLSVSVGIALYEASRQL 237
Cdd:cd18094    77 TTKGTKPYTDFDFKPGDYLVFGRESTGLPEEILDAFPDR-----RLRIPMRPK-VRSLNLSNSVAIVLYEALRQL 145
 
Name Accession Description Interval E-value
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 83-237 4.63e-83

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 245.05  E-value: 4.63e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  83 HVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARVKRAGLDYWPFVVVKAHSSWAEFQEYFRlqegEKRMIAF 162
Cdd:cd18094     1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALP----PGRLFLF 76

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1063729171 163 TKRGTRIHSDFSYRSGDYLLFGSETSGLPPEALSDCNHEpygggTLRIPMVETyVRCLNLSVSVGIALYEASRQL 237
Cdd:cd18094    77 TTKGTKPYTDFDFKPGDYLVFGRESTGLPEEILDAFPDR-----RLRIPMRPK-VRSLNLSNSVAIVLYEALRQL 145
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 82-239 1.39e-79

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 236.47  E-value: 1.39e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  82 LHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARVKRAGLDYWPFVVVKAHSSWAEFQEYFRlqegEKRMIA 161
Cdd:COG0219     2 LHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALP----GGRLFL 77

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1063729171 162 FTKRGTRIHSDFSYRSGDYLLFGSETSGLPPEALSDCNHEpygggTLRIPMVETyVRCLNLSVSVGIALYEASRQLNY 239
Cdd:COG0219    78 FTTKGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAAPADQ-----RLRIPMRPG-VRSLNLSNAVAIVLYEALRQLGF 149
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 81-239 3.38e-60

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 187.24  E-value: 3.38e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  81 LLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARVKRAGLDYWPFVVVKAHSSWAEFQEYFRlqegEKRMI 160
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEK----PQKLF 76

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1063729171 161 AFTKRGTRIHSDFSYRSGDYLLFGSETSGLPPEALsdcnhEPYGGGTLRIPMVETyVRCLNLSVSVGIALYEASRQLNY 239
Cdd:TIGR00185  77 ALTKKGTPAHSAVTYQDGDYLMFGPETRGLPQSIL-----DNLMEQKIRIPMTND-VRSLNLSNAVAIVVYEAWRQLGY 149
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 81-239 1.69e-45

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 150.15  E-value: 1.69e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  81 LLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARVKRAGLDYWPFVVVKAHSSWAEFQEyfrlQEGEKRMI 160
Cdd:PRK10358    1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLE----AENPQRLF 76

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1063729171 161 AFTKRGTRIHSDFSYRSGDYLLFGSETSGLPPEALSDCNHEPygggTLRIPMVETyVRCLNLSVSVGIALYEASRQLNY 239
Cdd:PRK10358   77 ALTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQ----KIRIPMMPD-SRSMNLSNAVSVVVYEAWRQLGY 150
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 82-231 2.28e-42

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 141.51  E-value: 2.28e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  82 LHVVLVSPQIPGNTGCIARTCAA-SAVGLHLVGPLGFQVDDARVKRAGLDYWPFVVVKAHSSWAEFQEYfrLQEGEKRMI 160
Cdd:pfam00588   2 LVVVLDNIQDPGNLGAIIRTAAAfGADGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKD--LKEAGYAVV 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1063729171 161 AFTKRGTRIHsDFSYRSGDYLLFGSETSGLPPEALSDCNHepygggTLRIPMVETYvRCLNLSVSVGIALY 231
Cdd:pfam00588  80 ATSLGGKSLY-EIDFPKKVALVFGNEGTGLSNEVLELCDR------LVRIPMSGDV-ESLNVAVAAAIALY 142
 
Name Accession Description Interval E-value
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 83-237 4.63e-83

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 245.05  E-value: 4.63e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  83 HVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARVKRAGLDYWPFVVVKAHSSWAEFQEYFRlqegEKRMIAF 162
Cdd:cd18094     1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALP----PGRLFLF 76

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1063729171 163 TKRGTRIHSDFSYRSGDYLLFGSETSGLPPEALSDCNHEpygggTLRIPMVETyVRCLNLSVSVGIALYEASRQL 237
Cdd:cd18094    77 TTKGTKPYTDFDFKPGDYLVFGRESTGLPEEILDAFPDR-----RLRIPMRPK-VRSLNLSNSVAIVLYEALRQL 145
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 82-239 1.39e-79

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 236.47  E-value: 1.39e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  82 LHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARVKRAGLDYWPFVVVKAHSSWAEFQEYFRlqegEKRMIA 161
Cdd:COG0219     2 LHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALP----GGRLFL 77

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1063729171 162 FTKRGTRIHSDFSYRSGDYLLFGSETSGLPPEALSDCNHEpygggTLRIPMVETyVRCLNLSVSVGIALYEASRQLNY 239
Cdd:COG0219    78 FTTKGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAAPADQ-----RLRIPMRPG-VRSLNLSNAVAIVLYEALRQLGF 149
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 81-239 3.38e-60

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 187.24  E-value: 3.38e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  81 LLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARVKRAGLDYWPFVVVKAHSSWAEFQEYFRlqegEKRMI 160
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEK----PQKLF 76

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1063729171 161 AFTKRGTRIHSDFSYRSGDYLLFGSETSGLPPEALsdcnhEPYGGGTLRIPMVETyVRCLNLSVSVGIALYEASRQLNY 239
Cdd:TIGR00185  77 ALTKKGTPAHSAVTYQDGDYLMFGPETRGLPQSIL-----DNLMEQKIRIPMTND-VRSLNLSNAVAIVVYEAWRQLGY 149
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 81-239 1.69e-45

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 150.15  E-value: 1.69e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  81 LLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARVKRAGLDYWPFVVVKAHSSWAEFQEyfrlQEGEKRMI 160
Cdd:PRK10358    1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLE----AENPQRLF 76

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1063729171 161 AFTKRGTRIHSDFSYRSGDYLLFGSETSGLPPEALSDCNHEPygggTLRIPMVETyVRCLNLSVSVGIALYEASRQLNY 239
Cdd:PRK10358   77 ALTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQ----KIRIPMMPD-SRSMNLSNAVSVVVYEAWRQLGY 150
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 82-231 2.28e-42

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 141.51  E-value: 2.28e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  82 LHVVLVSPQIPGNTGCIARTCAA-SAVGLHLVGPLGFQVDDARVKRAGLDYWPFVVVKAHSSWAEFQEYfrLQEGEKRMI 160
Cdd:pfam00588   2 LVVVLDNIQDPGNLGAIIRTAAAfGADGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKD--LKEAGYAVV 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1063729171 161 AFTKRGTRIHsDFSYRSGDYLLFGSETSGLPPEALSDCNHepygggTLRIPMVETYvRCLNLSVSVGIALY 231
Cdd:pfam00588  80 ATSLGGKSLY-EIDFPKKVALVFGNEGTGLSNEVLELCDR------LVRIPMSGDV-ESLNVAVAAAIALY 142
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 83-233 1.47e-28

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 105.77  E-value: 1.47e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  83 HVVLVSPQIPGNTGCIARTCAASAV-GLHLVGPLGFqVDDARVKRAGLDYW---PFVVVKahsswaEFQEYFR-LQEGEK 157
Cdd:cd18082     1 HLVLDNIRSPGNIGSIFRTADAFGVeKIYLVGPTAD-IFDPKAKRTSRGTHfsvPYERVD------NFEEYLKsLKEEGY 73

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1063729171 158 RMIAF--TKRGTRIHS-DFSYRSGDYLLFGSETSGLPPEALSDCNHepygggTLRIPMVETyVRCLNLSVSVGIALYEA 233
Cdd:cd18082    74 PLIGLelTDGSIPLSEfKFKKPKKIALIFGNERSGLSPELLALCDY------TVHIPMSGK-NSSLNVSVATGIALYEF 145
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 71-236 2.68e-14

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 70.61  E-value: 2.68e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  71 EGVKEDARSKLLHVVLVSPQIPGNTGCIARTCAA---SAVglhLVGPLGFQVDDARVKRA---GLDYWPFVVVkahSSWA 144
Cdd:COG0566   105 EDLLAEAGEPPLLLVLDGVQDPGNLGAILRSAAAfgvDGV---ILPKRRADPYNPKVVRAsagALFHVPVARV---TNLA 178

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171 145 EFQEyfRLQEGEKRMIAFTKRGTRIHSDFSYRSGDYLLFGSETSGLPPEALSDCNHepygggTLRIPM---VETyvrcLN 221
Cdd:COG0566   179 DALE--ELKEAGFWVVGTDLDGEASLYEVDLPGPVALVLGNEGKGLSRLVLEACDE------LVRIPMaggVES----LN 246

                         170
                  ....*....|....*
gi 1063729171 222 LSVSVGIALYEASRQ 236
Cdd:COG0566   247 VAVAAAILLYEAARQ 261
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 76-237 2.75e-13

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 65.95  E-value: 2.75e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  76 DARSKLLHVVLVSPQIPGNTGCIARTCaaSAVG---LHLVGPLGFQVDDARVKRaGLDYWpfVVVKAHSSWAEFQEYFRl 152
Cdd:cd18092    11 ARRTRDLTVVLENVHDPHNASAVLRTC--DAFGvqdVHVIEPKNKFKKNRGVAM-GADKW--LDVHRHDSTEECLAALK- 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171 153 QEGEKrmIAftkrGTRIHSD-FSYRSGDY-----LLFGSETSGLPPEALSDCNHepygggTLRIPMVeTYVRCLNLSVSV 226
Cdd:cd18092    85 EQGYR--IV----ATSLSPDaVPLDELDYtrptaLVFGNEKDGVSEEALAAADE------RVYIPMY-GFVESLNVSVAA 151

                         170
                  ....*....|.
gi 1063729171 227 GIALYEASRQL 237
Cdd:cd18092   152 AIILYELQRQR 162
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 82-232 2.07e-12

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 63.28  E-value: 2.07e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  82 LHVVLVSPQIPGNTGCIARTCAASAVG-LHLVGPLGFQVDDARVKRAgldywpfvvVKAHSSWAEFQEYFRLQE---GEK 157
Cdd:cd18093     1 IRVVLVEPQHPGNIGAVARAMKNFGLSdLRLVNPRCDDPEEAARALA---------SGAEDVLENAKVYDTLEEalaDCD 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171 158 RMIAFT--KRGTRIHS-------DFSYRSGDY----LLFGSETSGLPPEALSDCNHepygggTLRIPMVETYvRCLNLSV 224
Cdd:cd18093    72 LVVATTarPRGLRRPLltprelaELLKEHAAGgkvaLVFGREDSGLTNEELALCDL------LVTIPTNPEY-PSLNLAQ 144


                  ....*...
gi 1063729171 225 SVGIALYE 232
Cdd:cd18093   145 AVAIVLYE 152
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 84-233 4.80e-12

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 62.06  E-value: 4.80e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  84 VVLVSPQIPGNTGCIARTCAasAVGLHLVGPLGFQVD--DARVKRA---GLDYWPFVVVkahSSWAEFQEyfRLQEGEKR 158
Cdd:cd18095     3 LVLDGVQDPGNLGTIIRTAA--AFGVDGVILSKGTVDpyNPKVVRAsmgALFRLPIIRV---DDLEELLE--ELKEAGYK 75

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1063729171 159 MIAFTKRGTRIHSDFSYRSGDYLLFGSETSGLPPEALSDCNHepygggTLRIPM---VETyvrcLNLSVSVGIALYEA 233
Cdd:cd18095    76 VVAADLDGGSSLYDLDLPKPVALVLGNEGNGLSDELLELCDK------RVTIPMsggVES----LNVAVAAAILLYEL 143
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 92-233 8.16e-09

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 53.02  E-value: 8.16e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  92 PGNTGCIARTCAasAVGLHLVGPLGFQVD--DARVKRAGLDYWPFVVVKAHSSWAEFQEYFRlqEGEKRMIAFTKRGTRI 169
Cdd:cd18106    11 PGNLGSILRSAA--AAGCSKVLLTKGCVDpwEPKVLRAGAGAHFRLPIYGNLSWDDIPNYLP--DDSKVFLADNNLPVQP 86

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1063729171 170 HSDFSYRSGDYLLF-GSETSGLPPEALSDCnhEPYGGGTLRIPMVETyVRCLNLSVSVGIALYEA 233
Cdd:cd18106    87 YDSIDYCSGPIVLViGGETHGVSPEAYKLA--HSTGGSRVHIPLANG-VDSLNSAVAASILLFEM 148
SpoU-like_NHR cd18108
Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) ...
 84-232 2.81e-07

Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) confers resistance to the thiazole antibiotic nosiheptide via catalyzing 2'O-methylation of 23S rRNA at the nucleotide A1067. NHR is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349981  Cd Length: 144  Bit Score: 48.63  E-value: 2.81e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  84 VVLVSPQIPGNTGCIARTCAA-SAVGLHLVGPLGFQVDDARVKRA--GLDYWPFVVVkahsswAEFQEYFRLQEGEKRMI 160
Cdd:cd18108     3 VVLDGVRIVGNIGAIVRTSLAlGAAGIVLVDSDLATVADRRLLRAsrGYVFSLPVVL------ADREEAVAFLRDNDIPL 76

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1063729171 161 AFTKRGTR--IHSDFSYRSGDYLLFGSETSGLPPEAlsdcnhEPYGGGTLRIPMVeTYVRCLNLSVSVGIALYE 232
Cdd:cd18108    77 MTLDADGDvpVKELGDRPDRLALVFGSEKGGPSGLF------EEAAAGTVSIPMS-SSTESLNVSVSAGIALHE 143
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 82-237 4.21e-07

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 49.73  E-value: 4.21e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  82 LHVVLVSPQIPGNTGCIARTCAASAVG-LHLVGPLGFQVDDARvKRA--GLDYWPFVVVkaHSSWAEfqeyfrLQEGEKR 158
Cdd:COG0565     4 IRVVLVEPQHPGNIGAAARAMKNFGLSdLRLVNPRGWPDEEAR-AMAsgADDVLENARV--VDTLEE------ALADCDL 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171 159 MIAFT--KRGTRIH--------SDFSYRSGDY---LLFGSETSGLPPEALSDCNHepygggTLRIPMVETYvRCLNLSVS 225
Cdd:COG0565    75 VVGTTarPRELRKPvltpreaaEELLARAAGGkvaLVFGRERSGLTNEELALCDA------LVTIPTNPEY-PSLNLAQA 147

                         170
                  ....*....|..
gi 1063729171 226 VGIALYEASRQL 237
Cdd:COG0565   148 VQVVAYELRKAA 159
rRNA_methyl_3 TIGR00186
rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases ...
 72-237 3.29e-05

rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129290 [Multi-domain]  Cd Length: 237  Bit Score: 43.87  E-value: 3.29e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  72 GVKEDARSKLLhVVLVSPQIPGNTGCIARTCAASAVGL-----HLVGPLGFQVddARVKRAGLDYWPFVVVkahsswAEF 146
Cdd:TIGR00186  82 KTAKSKKQPFL-LILDEITDPHNLGAILRTAEAFGVDGvilpkRRSAPLNSTV--VKTSSGAVEYVPLARV------TNL 152

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171 147 QEYFR-LQEgekrmIAFTKRGTRIH-SDFSYRSGDY----LLFGSETSGLPPEALSDCNHepygggTLRIPMVETyVRCL 220
Cdd:TIGR00186 153 SRTITkLKE-----SGFWTVGTDLDaQDTLYQVKLTkplaLVVGNEGEGVSRLIKENCDF------LIKIPMAGK-VDSL 220

                         170
                  ....*....|....*..
gi 1063729171 221 NLSVSVGIALYEASRQL 237
Cdd:TIGR00186 221 NVSVAAGILLFEIKRQR 237
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 82-233 1.37e-04

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 41.22  E-value: 1.37e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  82 LHVVLVSPQIPGNTGCIARTCAasAVGLHLVGPLGFQVD--DARVKRAGLDYW---PFVVVkahSSWAEFQEYfrLQEGE 156
Cdd:cd18107     1 LVVLFDRPTSPGNLGTIIRSAD--ALGAHGLVVTGHAADvyDPKTIRASTGSLfslPIVRV---PSNRELEDW--VEARK 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171 157 KRMIAFTKRGTRIH-----SDFSYRSGDYLLFGSETSGLPPEALSDCNhepyggGTLRIPMVETyVRCLNLSVSVGIALY 231
Cdd:cd18107    74 ARGTPLQLVGTDEHgdhqiADHDFTRPTLLLIGNETAGLSAAWRELCD------ETVSIPMAGS-ASSLNAACAASIFLY 146


                  ..
gi 1063729171 232 EA 233
Cdd:cd18107   147 EA 148
SpoU-like cd18098
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 86-231 2.11e-04

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349971  Cd Length: 138  Bit Score: 40.32  E-value: 2.11e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063729171  86 LVSPQIPGNTGCIARTCAA-SAVGLHLVG----PLGFQVDDARVKRAgldyWPFVVVKahsSWAEFQEYfrlQEGEKRMI 160
Cdd:cd18098     5 IENPKTPKNVGTLWRAAGClGASFVFTIGarykRRKQSTDTSKAWRH----IPLFHYD---DLEDLAEP---LPFGAVLV 74

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1063729171 161 AF--TKRGTRIHsDFSYRSGDYLLFGSETSGLPPEALSDCNHepygggTLRIPmveTYvRCLNLSVSVGIALY 231
Cdd:cd18098    75 AVelTEGATPLP-DFKHPDRAVYLLGPEDGGLPQEVLARCHH------VVYLP---TR-GSLNVAVAGSIVLY 136
SpoU-like_MRM1 cd18105
SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose ...
 181-232 5.94e-03

SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose residues G1145 to GmG residue of the mitochondrial 16S rRNA. MRM1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349978  Cd Length: 158  Bit Score: 36.34  E-value: 5.94e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1063729171 181 LLFGSETSGLPPEALSDCNHepygggTLRIPMV----ETYVRCLNLSVSVGIALYE 232
Cdd:cd18105   108 LVLGNEGRGLRTNIKNLCDY------LVSIPGGrsaeNPGVDSLNVSVAAGILLHS 157
SpoU-like_RNA-MTase cd18109
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 165-232 9.93e-03

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349982  Cd Length: 141  Bit Score: 35.59  E-value: 9.93e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1063729171 165 RGTRIHSdFSYRSGDYLLFGSETSGLPPEALSDCNHepygggTLRIPMVETYVRCLNLSVSVGIALYE 232
Cdd:cd18109    80 DGENLYE-FTLPEKGILVMGNEGHGISPEVLKLLTH------RLTIPRYGKGAESLNVAVATAILLSE 140
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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