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Conserved domains on  [gi|1012282536|ref|NP_001308650|]
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sodium/nucleoside cotransporter 1 isoform 4 [Homo sapiens]

Protein Classification

NupC/NupG family nucleoside CNT transporter( domain architecture ID 11489677)

NupC/NupG family nucleoside CNT transporter such as nucleoside permease NupC, which transports nucleosides with a high affinity except guanosine and deoxyguanosine

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
nupC TIGR00804
nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) ...
179-512 1.36e-176

nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) Members of the CNT family mediate nucleoside uptake. In bacteria they are energized by H+ symport, but in mammals they are energized by Na+ symport. The different transporters exhibit differing specificities for nucleosides. The E. coli NupC permease transports all nucleosides (both ribo- and deoxyribonucleosides) except hypoxanthine and guanine nucleosides. The B. subtilis NupC is specific for pyrimidine nucleosides (cytidine and uridine and the corresponding deoxyribonucleosides). The mammalian permease members of the CNT family also exhibit differing specificities. Thus, rats possess at least two NupC homologues, one specific for both purine and pyrimidine nucleosides and one specific for purine nucleosides. At least three paralogues have been characterized from humans. One human homologue(CNT1) transports pyrimidine nucleosides and adenosine, but deoxyadenosine and guanosine are poor substrates of this permease. Another (CNT2) is selective for purine nucleosides. Alteration of just a few amino acyl residues in TMSs 7 and 8 interconverts their specificities. [Transport and binding proteins, Nucleosides, purines and pyrimidines]


:

Pssm-ID: 273278 [Multi-domain]  Cd Length: 401  Bit Score: 503.37  E-value: 1.36e-176
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 179 LVSFAGICVFVALLFACSKHHCAVSWRAVSWGLGLQFVLGLLVIRTEPGFIAFEWLGEQIRIFLSYTKAGSSFVFGEALV 258
Cdd:TIGR00804   1 LISFVGLVVFILIAFLCSSNKKAISWRTVVSALVLQFLLGLIVLRTPPGFWAFQGLADQIQILLSYANEGSSFVFGPPLV 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 259 KD--VFAFQVLPIIVFFSCVISVLYHVGLMQWVILKIAWLMQVTMGTTATETLSVAGNIFVSQTEAPLLIRPYLADMTLS 336
Cdd:TIGR00804  81 QDlfVFAFQVLPIIIFFSALISILYYLGIMQWVIRKIGWFLQKAMGTTKLESFSAAANIFLGQTEAPLVIRPYLGDMTDS 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 337 EVHVVMTGGYATIAGSLLGAYISFGIDATSLIAASVMAAPCALALSKLVYPEVEESKFRREEGVKLTYGDaQNLIEAAST 416
Cdd:TIGR00804 161 ELFTVMTSGMATIAGSVLGAYISMGVPATYLIAASVMAAPCALAFAKLIYPEVEESKEKSEEDVKLEEGD-QNFFEAASN 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 417 GAAISVKVVANIAANLIAFLAVLDFINAALSWLGDMVDIQGLSFQLICSYILRPVAFLMGVAWEDCPVVAELLGIKLFLN 496
Cdd:TIGR00804 240 GALAGVKVVANVAAMLIAFVALLALINGILSWVGGWVGYGGLSFQLIFGYVFRPLAFLMGVPWSDAPLVAQLMGTKLAVN 319
                         330
                  ....*....|....*.
gi 1012282536 497 EFVAYQDLSKYKQRRL 512
Cdd:TIGR00804 320 EFVAYLDLSQYLQTRL 335
 
Name Accession Description Interval E-value
nupC TIGR00804
nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) ...
179-512 1.36e-176

nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) Members of the CNT family mediate nucleoside uptake. In bacteria they are energized by H+ symport, but in mammals they are energized by Na+ symport. The different transporters exhibit differing specificities for nucleosides. The E. coli NupC permease transports all nucleosides (both ribo- and deoxyribonucleosides) except hypoxanthine and guanine nucleosides. The B. subtilis NupC is specific for pyrimidine nucleosides (cytidine and uridine and the corresponding deoxyribonucleosides). The mammalian permease members of the CNT family also exhibit differing specificities. Thus, rats possess at least two NupC homologues, one specific for both purine and pyrimidine nucleosides and one specific for purine nucleosides. At least three paralogues have been characterized from humans. One human homologue(CNT1) transports pyrimidine nucleosides and adenosine, but deoxyadenosine and guanosine are poor substrates of this permease. Another (CNT2) is selective for purine nucleosides. Alteration of just a few amino acyl residues in TMSs 7 and 8 interconverts their specificities. [Transport and binding proteins, Nucleosides, purines and pyrimidines]


Pssm-ID: 273278 [Multi-domain]  Cd Length: 401  Bit Score: 503.37  E-value: 1.36e-176
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 179 LVSFAGICVFVALLFACSKHHCAVSWRAVSWGLGLQFVLGLLVIRTEPGFIAFEWLGEQIRIFLSYTKAGSSFVFGEALV 258
Cdd:TIGR00804   1 LISFVGLVVFILIAFLCSSNKKAISWRTVVSALVLQFLLGLIVLRTPPGFWAFQGLADQIQILLSYANEGSSFVFGPPLV 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 259 KD--VFAFQVLPIIVFFSCVISVLYHVGLMQWVILKIAWLMQVTMGTTATETLSVAGNIFVSQTEAPLLIRPYLADMTLS 336
Cdd:TIGR00804  81 QDlfVFAFQVLPIIIFFSALISILYYLGIMQWVIRKIGWFLQKAMGTTKLESFSAAANIFLGQTEAPLVIRPYLGDMTDS 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 337 EVHVVMTGGYATIAGSLLGAYISFGIDATSLIAASVMAAPCALALSKLVYPEVEESKFRREEGVKLTYGDaQNLIEAAST 416
Cdd:TIGR00804 161 ELFTVMTSGMATIAGSVLGAYISMGVPATYLIAASVMAAPCALAFAKLIYPEVEESKEKSEEDVKLEEGD-QNFFEAASN 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 417 GAAISVKVVANIAANLIAFLAVLDFINAALSWLGDMVDIQGLSFQLICSYILRPVAFLMGVAWEDCPVVAELLGIKLFLN 496
Cdd:TIGR00804 240 GALAGVKVVANVAAMLIAFVALLALINGILSWVGGWVGYGGLSFQLIFGYVFRPLAFLMGVPWSDAPLVAQLMGTKLAVN 319
                         330
                  ....*....|....*.
gi 1012282536 497 EFVAYQDLSKYKQRRL 512
Cdd:TIGR00804 320 EFVAYLDLSQYLQTRL 335
NupC COG1972
Nucleoside permease NupC [Nucleotide transport and metabolism];
179-508 8.22e-125

Nucleoside permease NupC [Nucleotide transport and metabolism];


Pssm-ID: 441575 [Multi-domain]  Cd Length: 406  Bit Score: 371.73  E-value: 8.22e-125
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 179 LVSFAGICVFVALLFACSKHHCAVSWRAVSWGLGLQFVLGLLVIRTEPGFIAFEWLGEQIRIFLSYTKAGSSFVFGEALV 258
Cdd:COG1972     4 LIGLLGIAVLLGIAWLFSENRKAINWRTVGIGLALQLLLAFLVLKVPAGRAAFEALSDGVNKLLSYANEGTAFVFGGLAG 83
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 259 KD-----VFAFQVLPIIVFFSCVISVLYHVGLMQWVILKIAWLMQVTMGTTATETLSVAGNIFVSQTEAPLLIRPYLADM 333
Cdd:COG1972    84 PGlpggfVFAFQVLPPIIFFSALISILYYLGILQFIVRGIGWVLSKILGTSGAESLSAAANIFLGQTEAPLVIKPYLAKM 163
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 334 TLSEVHVVMTGGYATIAGSLLGAYISF-GIDATSLIAASVMAAPCALALSKLVYPEVEESKFRREEGVKLTYgDAQNLIE 412
Cdd:COG1972   164 TRSELFTVMTGGMATVAGSVLAAYASMlGVPAKYLLAASVMAAPAALLIAKILVPETEEPETDGVVEVEEEK-EPANFID 242
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 413 AASTGAAISVKVVANIAANLIAFLAVLDFINAALSWLGDMVDiqGLSFQLICSYILRPVAFLMGVAWEDCPVVAELLGIK 492
Cdd:COG1972   243 AIAEGALDGLKLALNVGAMLIAFVALIALVNGILGGLGGLFG--GLSLQRILGYLFAPLAWLIGVPWSEALQAGSLMGTK 320
                         330
                  ....*....|....*.
gi 1012282536 493 LFLNEFVAYQDLSKYK 508
Cdd:COG1972   321 LVLNEFVAYLDLAKLL 336
Nucleos_tra2_C pfam07662
Na+ dependent nucleoside transporter C-terminus; This family consists of nucleoside transport ...
367-535 5.15e-61

Na+ dependent nucleoside transporter C-terminus; This family consists of nucleoside transport proteins. Swiss:Q62773 is a purine-specific Na+-nucleoside cotransporter localized to the bile canalicular membrane. Swiss:Q62674 is a a Na+-dependent nucleoside transporter selective for pyrimidine nucleosides and adenosine it also transports the anti-viral nucleoside analogues AZT and ddC. This alignment covers the C-terminus of this family of transporters.


Pssm-ID: 462223  Cd Length: 205  Bit Score: 199.93  E-value: 5.15e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 367 LIAASVMAAPCALALSKLVYPEVEESKFRREEGvkltyGDAQNLIEAASTGAAISVKVVANIAANLIAFLAVLDFINAAL 446
Cdd:pfam07662   2 LLTASVMSAPAALAISKLLYPETEEPPVKTGDE-----EEPANVLDAASNGALDGLKLALNIGAMLIAFVALVALINGLL 76
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 447 SWLGDMVDIQGLSFQLICSYILRPVAFLMGVAWEDCPVVAELLGIKLFLNEFVAYQDLSKYKQrrlagaeewvgdrKQWI 526
Cdd:pfam07662  77 GWIGGLFGFPGLSLQLILGYVFAPLAWLMGVPWDDALQVGSLMGTKLVLNEFVAYLDLGKLIA-------------AGAL 143

                  ....*....
gi 1012282536 527 SRRGLAMLT 535
Cdd:pfam07662 144 SPRSVAIAT 152
 
Name Accession Description Interval E-value
nupC TIGR00804
nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) ...
179-512 1.36e-176

nucleoside transporter; The Concentrative Nucleoside Transporter (CNT) Family (TC 2.A.41) Members of the CNT family mediate nucleoside uptake. In bacteria they are energized by H+ symport, but in mammals they are energized by Na+ symport. The different transporters exhibit differing specificities for nucleosides. The E. coli NupC permease transports all nucleosides (both ribo- and deoxyribonucleosides) except hypoxanthine and guanine nucleosides. The B. subtilis NupC is specific for pyrimidine nucleosides (cytidine and uridine and the corresponding deoxyribonucleosides). The mammalian permease members of the CNT family also exhibit differing specificities. Thus, rats possess at least two NupC homologues, one specific for both purine and pyrimidine nucleosides and one specific for purine nucleosides. At least three paralogues have been characterized from humans. One human homologue(CNT1) transports pyrimidine nucleosides and adenosine, but deoxyadenosine and guanosine are poor substrates of this permease. Another (CNT2) is selective for purine nucleosides. Alteration of just a few amino acyl residues in TMSs 7 and 8 interconverts their specificities. [Transport and binding proteins, Nucleosides, purines and pyrimidines]


Pssm-ID: 273278 [Multi-domain]  Cd Length: 401  Bit Score: 503.37  E-value: 1.36e-176
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 179 LVSFAGICVFVALLFACSKHHCAVSWRAVSWGLGLQFVLGLLVIRTEPGFIAFEWLGEQIRIFLSYTKAGSSFVFGEALV 258
Cdd:TIGR00804   1 LISFVGLVVFILIAFLCSSNKKAISWRTVVSALVLQFLLGLIVLRTPPGFWAFQGLADQIQILLSYANEGSSFVFGPPLV 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 259 KD--VFAFQVLPIIVFFSCVISVLYHVGLMQWVILKIAWLMQVTMGTTATETLSVAGNIFVSQTEAPLLIRPYLADMTLS 336
Cdd:TIGR00804  81 QDlfVFAFQVLPIIIFFSALISILYYLGIMQWVIRKIGWFLQKAMGTTKLESFSAAANIFLGQTEAPLVIRPYLGDMTDS 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 337 EVHVVMTGGYATIAGSLLGAYISFGIDATSLIAASVMAAPCALALSKLVYPEVEESKFRREEGVKLTYGDaQNLIEAAST 416
Cdd:TIGR00804 161 ELFTVMTSGMATIAGSVLGAYISMGVPATYLIAASVMAAPCALAFAKLIYPEVEESKEKSEEDVKLEEGD-QNFFEAASN 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 417 GAAISVKVVANIAANLIAFLAVLDFINAALSWLGDMVDIQGLSFQLICSYILRPVAFLMGVAWEDCPVVAELLGIKLFLN 496
Cdd:TIGR00804 240 GALAGVKVVANVAAMLIAFVALLALINGILSWVGGWVGYGGLSFQLIFGYVFRPLAFLMGVPWSDAPLVAQLMGTKLAVN 319
                         330
                  ....*....|....*.
gi 1012282536 497 EFVAYQDLSKYKQRRL 512
Cdd:TIGR00804 320 EFVAYLDLSQYLQTRL 335
NupC COG1972
Nucleoside permease NupC [Nucleotide transport and metabolism];
179-508 8.22e-125

Nucleoside permease NupC [Nucleotide transport and metabolism];


Pssm-ID: 441575 [Multi-domain]  Cd Length: 406  Bit Score: 371.73  E-value: 8.22e-125
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 179 LVSFAGICVFVALLFACSKHHCAVSWRAVSWGLGLQFVLGLLVIRTEPGFIAFEWLGEQIRIFLSYTKAGSSFVFGEALV 258
Cdd:COG1972     4 LIGLLGIAVLLGIAWLFSENRKAINWRTVGIGLALQLLLAFLVLKVPAGRAAFEALSDGVNKLLSYANEGTAFVFGGLAG 83
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 259 KD-----VFAFQVLPIIVFFSCVISVLYHVGLMQWVILKIAWLMQVTMGTTATETLSVAGNIFVSQTEAPLLIRPYLADM 333
Cdd:COG1972    84 PGlpggfVFAFQVLPPIIFFSALISILYYLGILQFIVRGIGWVLSKILGTSGAESLSAAANIFLGQTEAPLVIKPYLAKM 163
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 334 TLSEVHVVMTGGYATIAGSLLGAYISF-GIDATSLIAASVMAAPCALALSKLVYPEVEESKFRREEGVKLTYgDAQNLIE 412
Cdd:COG1972   164 TRSELFTVMTGGMATVAGSVLAAYASMlGVPAKYLLAASVMAAPAALLIAKILVPETEEPETDGVVEVEEEK-EPANFID 242
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 413 AASTGAAISVKVVANIAANLIAFLAVLDFINAALSWLGDMVDiqGLSFQLICSYILRPVAFLMGVAWEDCPVVAELLGIK 492
Cdd:COG1972   243 AIAEGALDGLKLALNVGAMLIAFVALIALVNGILGGLGGLFG--GLSLQRILGYLFAPLAWLIGVPWSEALQAGSLMGTK 320
                         330
                  ....*....|....*.
gi 1012282536 493 LFLNEFVAYQDLSKYK 508
Cdd:COG1972   321 LVLNEFVAYLDLAKLL 336
Nucleos_tra2_C pfam07662
Na+ dependent nucleoside transporter C-terminus; This family consists of nucleoside transport ...
367-535 5.15e-61

Na+ dependent nucleoside transporter C-terminus; This family consists of nucleoside transport proteins. Swiss:Q62773 is a purine-specific Na+-nucleoside cotransporter localized to the bile canalicular membrane. Swiss:Q62674 is a a Na+-dependent nucleoside transporter selective for pyrimidine nucleosides and adenosine it also transports the anti-viral nucleoside analogues AZT and ddC. This alignment covers the C-terminus of this family of transporters.


Pssm-ID: 462223  Cd Length: 205  Bit Score: 199.93  E-value: 5.15e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 367 LIAASVMAAPCALALSKLVYPEVEESKFRREEGvkltyGDAQNLIEAASTGAAISVKVVANIAANLIAFLAVLDFINAAL 446
Cdd:pfam07662   2 LLTASVMSAPAALAISKLLYPETEEPPVKTGDE-----EEPANVLDAASNGALDGLKLALNIGAMLIAFVALVALINGLL 76
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 447 SWLGDMVDIQGLSFQLICSYILRPVAFLMGVAWEDCPVVAELLGIKLFLNEFVAYQDLSKYKQrrlagaeewvgdrKQWI 526
Cdd:pfam07662  77 GWIGGLFGFPGLSLQLILGYVFAPLAWLMGVPWDDALQVGSLMGTKLVLNEFVAYLDLGKLIA-------------AGAL 143

                  ....*....
gi 1012282536 527 SRRGLAMLT 535
Cdd:pfam07662 144 SPRSVAIAT 152
Nucleos_tra2_N pfam01773
Na+ dependent nucleoside transporter N-terminus; This family consists of nucleoside transport ...
184-255 3.31e-23

Na+ dependent nucleoside transporter N-terminus; This family consists of nucleoside transport proteins. Swiss:Q62773 is a purine-specific Na+-nucleoside cotransporter localized to the bile canalicular membrane. Swiss:Q62674 is a a Na+-dependent nucleoside transporter selective for pyrimidine nucleosides and adenosine it also transports the anti-viral nucleoside analogues AZT and ddC. This alignment covers the N terminus of this family


Pssm-ID: 460319 [Multi-domain]  Cd Length: 73  Bit Score: 93.21  E-value: 3.31e-23
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1012282536 184 GICVFVALLFACSKHHCAVSWRAVSWGLGLQFVLGLLVIRTEPGFIAFEWLGEQIRIFLSYTKAGSSFVFGE 255
Cdd:pfam01773   1 GLLVLLGIAWLFSKNRKAINWRTVIGGLALQFLLALFVLRTPAGRDAFKWISDGVTALLGYADAGTAFVFGG 72
Gate pfam07670
Nucleoside recognition; This region in the nucleoside transporter proteins are responsible for ...
263-361 8.40e-13

Nucleoside recognition; This region in the nucleoside transporter proteins are responsible for determining nucleoside specificity in the human CNT1 and CNT2 proteins. In the FeoB proteins, which are believed to be Fe2+ transporters, it includes the membrane pore region, so the function of this region is likely to be more general than just nucleoside specificity. This family may represent the pore and gate, with a wide potential range of specificity. Hence its name 'Gate'.


Pssm-ID: 429586 [Multi-domain]  Cd Length: 101  Bit Score: 64.58  E-value: 8.40e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1012282536 263 AFQVLPIIVFFSCVISVLYHVGLMQWVILKIAWLMQVtMGTTAtETLSVAGNIFVSQTE----APLLIRPYLADMTLSEV 338
Cdd:pfam07670   1 LLKVLPIILFFSVLISILEYSGLLDRIGKLLGPLMRP-LGLFP-LPGKAAIALLLGFGAkevgVPLLATPYGIDTPRERL 78
                          90       100
                  ....*....|....*....|...
gi 1012282536 339 HVVMTGGYATIAGSLLGAYISFG 361
Cdd:pfam07670  79 AALLFTSFSTPCGATLPVYAGEL 101
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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