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Conserved domains on  [gi|573459756|ref|NP_001275958|]
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protein-lysine N-methyltransferase EEF2KMT isoform 3 [Homo sapiens]

Protein Classification

protein-lysine N-methyltransferase EEF2KMT family protein( domain architecture ID 161358)

protein-lysine N-methyltransferase EEF2KMT family protein is a class I SAM-dependent methyltransferase, similar to EEF2KMT that catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'

CATH:  2.20.25.110
EC:  2.1.1.-
Gene Ontology:  GO:0008168|GO:1904047
PubMed:  25231979|12504684|12826405
SCOP:  3000118

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FAM86 super family cl20793
Family of unknown function; Function of this protein family is not known.
 6-53 7.00e-18

Family of unknown function; Function of this protein family is not known.


The actual alignment was detected with superfamily member pfam14904:

Pssm-ID: 464362  Cd Length: 94  Bit Score: 76.62  E-value: 7.00e-18
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 573459756    6 NAGTELLLQSFERRFLAARTLRSFPWQSLEAKLRDSSDSELLRDILHK 53
Cdd:pfam14904   1 SAETERVLREFQRRFLAMRRLRSFPWQSLEEELQNSSSSELLLDILQK 48
AdoMet_MTases super family cl17173
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 74-225 2.07e-17

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


The actual alignment was detected with superfamily member pfam10294:

Pssm-ID: 473071  Cd Length: 172  Bit Score: 77.37  E-value: 2.07e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573459756   74 GLVTWDAALYLAEW-----AIENPAV-FTNRTVLELGSGAGLTGLAICKMCRPRAYIFSDcHSRVLEQLRGNVLLNGLsl 147
Cdd:pfam10294  18 GGHVWDAAVVLSKYlemkiFKELGANnLSGLNVLELGSGTGLVGIAVALLLPGASVTITD-LEEALELLKKNIELNAL-- 94

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 573459756  148 eaditakldSPRVTVAQLDWDVATVHQLSAFQP-DVVIAADVLYCPEAIMSLVGVLRRLAacrehQRAPEVYVAFTVRN 225
Cdd:pfam10294  95 ---------SSKVVVKVLDWGENLPPDLFDGHPvDLILAADCVYNEDSFPLLEKTLKDLL-----GKESVILVAYKKRR 159
 
Name Accession Description Interval E-value
FAM86 pfam14904
Family of unknown function; Function of this protein family is not known.
 6-53 7.00e-18

Family of unknown function; Function of this protein family is not known.


Pssm-ID: 464362  Cd Length: 94  Bit Score: 76.62  E-value: 7.00e-18
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 573459756    6 NAGTELLLQSFERRFLAARTLRSFPWQSLEAKLRDSSDSELLRDILHK 53
Cdd:pfam14904   1 SAETERVLREFQRRFLAMRRLRSFPWQSLEEELQNSSSSELLLDILQK 48
Methyltransf_16 pfam10294
Lysine methyltransferase; Methyltrans_16 is a lysine methyltransferase. characterized members ...
 74-225 2.07e-17

Lysine methyltransferase; Methyltrans_16 is a lysine methyltransferase. characterized members of this family are protein methyltransferases targetting Lys residues in specific proteins, including calmodulin, VCP, Kin17 and Hsp70 proteins.


Pssm-ID: 313513  Cd Length: 172  Bit Score: 77.37  E-value: 2.07e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573459756   74 GLVTWDAALYLAEW-----AIENPAV-FTNRTVLELGSGAGLTGLAICKMCRPRAYIFSDcHSRVLEQLRGNVLLNGLsl 147
Cdd:pfam10294  18 GGHVWDAAVVLSKYlemkiFKELGANnLSGLNVLELGSGTGLVGIAVALLLPGASVTITD-LEEALELLKKNIELNAL-- 94

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 573459756  148 eaditakldSPRVTVAQLDWDVATVHQLSAFQP-DVVIAADVLYCPEAIMSLVGVLRRLAacrehQRAPEVYVAFTVRN 225
Cdd:pfam10294  95 ---------SSKVVVKVLDWGENLPPDLFDGHPvDLILAADCVYNEDSFPLLEKTLKDLL-----GKESVILVAYKKRR 159
 
Name Accession Description Interval E-value
FAM86 pfam14904
Family of unknown function; Function of this protein family is not known.
 6-53 7.00e-18

Family of unknown function; Function of this protein family is not known.


Pssm-ID: 464362  Cd Length: 94  Bit Score: 76.62  E-value: 7.00e-18
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 573459756    6 NAGTELLLQSFERRFLAARTLRSFPWQSLEAKLRDSSDSELLRDILHK 53
Cdd:pfam14904   1 SAETERVLREFQRRFLAMRRLRSFPWQSLEEELQNSSSSELLLDILQK 48
Methyltransf_16 pfam10294
Lysine methyltransferase; Methyltrans_16 is a lysine methyltransferase. characterized members ...
 74-225 2.07e-17

Lysine methyltransferase; Methyltrans_16 is a lysine methyltransferase. characterized members of this family are protein methyltransferases targetting Lys residues in specific proteins, including calmodulin, VCP, Kin17 and Hsp70 proteins.


Pssm-ID: 313513  Cd Length: 172  Bit Score: 77.37  E-value: 2.07e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573459756   74 GLVTWDAALYLAEW-----AIENPAV-FTNRTVLELGSGAGLTGLAICKMCRPRAYIFSDcHSRVLEQLRGNVLLNGLsl 147
Cdd:pfam10294  18 GGHVWDAAVVLSKYlemkiFKELGANnLSGLNVLELGSGTGLVGIAVALLLPGASVTITD-LEEALELLKKNIELNAL-- 94

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 573459756  148 eaditakldSPRVTVAQLDWDVATVHQLSAFQP-DVVIAADVLYCPEAIMSLVGVLRRLAacrehQRAPEVYVAFTVRN 225
Cdd:pfam10294  95 ---------SSKVVVKVLDWGENLPPDLFDGHPvDLILAADCVYNEDSFPLLEKTLKDLL-----GKESVILVAYKKRR 159
Methyltransf_12 pfam08242
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 101-207 4.08e-03

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 400515 [Multi-domain]  Cd Length: 98  Bit Score: 35.81  E-value: 4.08e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 573459756  101 LELGSGAGLTGLAICKMCRPRAYIFSDCHSRVLEQLRGNVllnglsleaditakLDSPRVTVAQLDWDVATVHQLSAFQP 180
Cdd:pfam08242   1 LEIGCGTGTLLRALLEALPGLEYTGLDISPAALEAARERL--------------AALGLLNAVRVELFQLDLGELDPGSF 66

                          90       100
                  ....*....|....*....|....*..
gi 573459756  181 DVVIAADVLYCPEAIMSLVGVLRRLAA 207
Cdd:pfam08242  67 DVVVASNVLHHLADPRAVLRNIRRLLK 93
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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