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Conserved domains on  [gi|384871624|ref|NP_001245177|]
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transcription factor Sp6 [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SP6_N cd22544
N-terminal domain of transcription factor Specificity Protein (SP) 6; Specificity Proteins ...
 1-255 1.05e-113

N-terminal domain of transcription factor Specificity Protein (SP) 6; Specificity Proteins (SPs) are transcription factors that are involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. SP6, also known as epiprofin, shows specific expression pattern in hair follicles and the apical ectodermal ridge (AER) of the developing limbs. SP6 null mice are nude and show defects in skin, teeth, limbs (syndactyly and oligodactyly), and lung alveoli. SP6 belongs to a family of proteins, called the SP/Kruppel or Krueppel-like Factor (KLF) family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. SP factors preferentially bind GC boxes, while KLFs bind CACCC boxes. Another characteristic hallmark of SP factors is the presence of the Buttonhead (BTD) box CXCPXC, just N-terminal to the zinc fingers. The function of the BTD box is unknown, but it is thought to play an important physiological role. Another feature of most SP factors is the presence of a conserved amino acid stretch, the so-called SP box, located close to the N-terminus. SP factors may be separated into three groups based on their domain architecture and the similarity of their N-terminal transactivation domains: SP1-4, SP5, and SP6-9. The transactivation domains between the three groups are not homologous to one another. This model represents the N-terminal domain of SP6.


:

Pssm-ID: 411693 [Multi-domain]  Cd Length: 245  Bit Score: 331.11  E-value: 1.05e-113
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624   1 MLTAVCGSLGSQHTEAPHASPPRLDLQPLQTYQGHTSPEAGDYPSPLQPGELQSLPLGPEVDFSQGYELPGASSRVTCED 80
Cdd:cd22544    1 MLTAVCGSLGNQHSETPRASPPTLDLQPLQPYQIHSSPEAGDYPSPLQPTELQSLPLGPGVDFSARESYEPHSSRRTCLD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624  81 LESDSPLAPGPFSKLLQPDMSHHYESWFRPTHPG-----AEDGSWWDLHPGTSWMDLPHTQGALTSPGHPGALQAGLGGY 155
Cdd:cd22544   81 LESDLPLGPFPKLLHPPPDMAHPYESWFRPPHPGgsgeeGGVPSWWDLHAGSSWMDLQHGQGGLQSPGPPGGLQPPLGGY 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 156 VGDHQLCAPPPHPHAHhllpaagGQHLLGPPDGAKALEVAapesqgLDSSLDGAARPKGSRRSVPRSSGQTVCRCPNCLE 235
Cdd:cd22544  161 GSEHQLCGPPHHLLPP-------AQHLMGQEGPKLLEHPA------EDPSLDGSPRPKGSRRSVPRSSGQAACRCPNCQE 227

                        250       260
                 ....*....|....*....|
gi 384871624 236 AERLGAPcgPDGGKKKHLHN 255
Cdd:cd22544  228 AERLGPP--PDGGKKKHLHN 245
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
261-336 4.67e-05

FOG: Zn-finger [General function prediction only];


:

Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 45.07  E-value: 4.67e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 261 CGKAYAKTSHLKAHLRW--HSG--DRPFVCNWLFCGKRFTRSDELQRHLQTHTGTKKFPCavCSRVFMRSDHLAKHMKTH 336
Cdd:COG5048  295 CNISFSRSSPLTRHLRSvnHSGesLKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKE--KLLNSSSKFSPLLNNEPP 372
 
Name Accession Description Interval E-value
SP6_N cd22544
N-terminal domain of transcription factor Specificity Protein (SP) 6; Specificity Proteins ...
 1-255 1.05e-113

N-terminal domain of transcription factor Specificity Protein (SP) 6; Specificity Proteins (SPs) are transcription factors that are involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. SP6, also known as epiprofin, shows specific expression pattern in hair follicles and the apical ectodermal ridge (AER) of the developing limbs. SP6 null mice are nude and show defects in skin, teeth, limbs (syndactyly and oligodactyly), and lung alveoli. SP6 belongs to a family of proteins, called the SP/Kruppel or Krueppel-like Factor (KLF) family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. SP factors preferentially bind GC boxes, while KLFs bind CACCC boxes. Another characteristic hallmark of SP factors is the presence of the Buttonhead (BTD) box CXCPXC, just N-terminal to the zinc fingers. The function of the BTD box is unknown, but it is thought to play an important physiological role. Another feature of most SP factors is the presence of a conserved amino acid stretch, the so-called SP box, located close to the N-terminus. SP factors may be separated into three groups based on their domain architecture and the similarity of their N-terminal transactivation domains: SP1-4, SP5, and SP6-9. The transactivation domains between the three groups are not homologous to one another. This model represents the N-terminal domain of SP6.


Pssm-ID: 411693 [Multi-domain]  Cd Length: 245  Bit Score: 331.11  E-value: 1.05e-113
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624   1 MLTAVCGSLGSQHTEAPHASPPRLDLQPLQTYQGHTSPEAGDYPSPLQPGELQSLPLGPEVDFSQGYELPGASSRVTCED 80
Cdd:cd22544    1 MLTAVCGSLGNQHSETPRASPPTLDLQPLQPYQIHSSPEAGDYPSPLQPTELQSLPLGPGVDFSARESYEPHSSRRTCLD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624  81 LESDSPLAPGPFSKLLQPDMSHHYESWFRPTHPG-----AEDGSWWDLHPGTSWMDLPHTQGALTSPGHPGALQAGLGGY 155
Cdd:cd22544   81 LESDLPLGPFPKLLHPPPDMAHPYESWFRPPHPGgsgeeGGVPSWWDLHAGSSWMDLQHGQGGLQSPGPPGGLQPPLGGY 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 156 VGDHQLCAPPPHPHAHhllpaagGQHLLGPPDGAKALEVAapesqgLDSSLDGAARPKGSRRSVPRSSGQTVCRCPNCLE 235
Cdd:cd22544  161 GSEHQLCGPPHHLLPP-------AQHLMGQEGPKLLEHPA------EDPSLDGSPRPKGSRRSVPRSSGQAACRCPNCQE 227

                        250       260
                 ....*....|....*....|
gi 384871624 236 AERLGAPcgPDGGKKKHLHN 255
Cdd:cd22544  228 AERLGPP--PDGGKKKHLHN 245
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
261-336 4.67e-05

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 45.07  E-value: 4.67e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 261 CGKAYAKTSHLKAHLRW--HSG--DRPFVCNWLFCGKRFTRSDELQRHLQTHTGTKKFPCavCSRVFMRSDHLAKHMKTH 336
Cdd:COG5048  295 CNISFSRSSPLTRHLRSvnHSGesLKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKE--KLLNSSSKFSPLLNNEPP 372
zf-H2C2_2 pfam13465
Zinc-finger double domain;
300-323 3.15e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 37.74  E-value: 3.15e-04
                          10        20
                  ....*....|....*....|....
gi 384871624  300 ELQRHLQTHTGTKKFPCAVCSRVF 323
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSF 24
 
Name Accession Description Interval E-value
SP6_N cd22544
N-terminal domain of transcription factor Specificity Protein (SP) 6; Specificity Proteins ...
 1-255 1.05e-113

N-terminal domain of transcription factor Specificity Protein (SP) 6; Specificity Proteins (SPs) are transcription factors that are involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. SP6, also known as epiprofin, shows specific expression pattern in hair follicles and the apical ectodermal ridge (AER) of the developing limbs. SP6 null mice are nude and show defects in skin, teeth, limbs (syndactyly and oligodactyly), and lung alveoli. SP6 belongs to a family of proteins, called the SP/Kruppel or Krueppel-like Factor (KLF) family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. SP factors preferentially bind GC boxes, while KLFs bind CACCC boxes. Another characteristic hallmark of SP factors is the presence of the Buttonhead (BTD) box CXCPXC, just N-terminal to the zinc fingers. The function of the BTD box is unknown, but it is thought to play an important physiological role. Another feature of most SP factors is the presence of a conserved amino acid stretch, the so-called SP box, located close to the N-terminus. SP factors may be separated into three groups based on their domain architecture and the similarity of their N-terminal transactivation domains: SP1-4, SP5, and SP6-9. The transactivation domains between the three groups are not homologous to one another. This model represents the N-terminal domain of SP6.


Pssm-ID: 411693 [Multi-domain]  Cd Length: 245  Bit Score: 331.11  E-value: 1.05e-113
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624   1 MLTAVCGSLGSQHTEAPHASPPRLDLQPLQTYQGHTSPEAGDYPSPLQPGELQSLPLGPEVDFSQGYELPGASSRVTCED 80
Cdd:cd22544    1 MLTAVCGSLGNQHSETPRASPPTLDLQPLQPYQIHSSPEAGDYPSPLQPTELQSLPLGPGVDFSARESYEPHSSRRTCLD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624  81 LESDSPLAPGPFSKLLQPDMSHHYESWFRPTHPG-----AEDGSWWDLHPGTSWMDLPHTQGALTSPGHPGALQAGLGGY 155
Cdd:cd22544   81 LESDLPLGPFPKLLHPPPDMAHPYESWFRPPHPGgsgeeGGVPSWWDLHAGSSWMDLQHGQGGLQSPGPPGGLQPPLGGY 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 156 VGDHQLCAPPPHPHAHhllpaagGQHLLGPPDGAKALEVAapesqgLDSSLDGAARPKGSRRSVPRSSGQTVCRCPNCLE 235
Cdd:cd22544  161 GSEHQLCGPPHHLLPP-------AQHLMGQEGPKLLEHPA------EDPSLDGSPRPKGSRRSVPRSSGQAACRCPNCQE 227

                        250       260
                 ....*....|....*....|
gi 384871624 236 AERLGAPcgPDGGKKKHLHN 255
Cdd:cd22544  228 AERLGPP--PDGGKKKHLHN 245
SP6-9_N cd22543
N-terminal domains of transcription factor Specificity Proteins (SP) 6-9, and similar proteins; ...
 1-255 1.51e-43

N-terminal domains of transcription factor Specificity Proteins (SP) 6-9, and similar proteins; Specificity Proteins (SPs) are transcription factors that are involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. There are many SPs in vertebrates (9 SPs in humans and mice, 7 SPs in the chicken, and 11 SPs in teleost fish), but arthropods only have 3 SPs. SPs belongs to a family of proteins, called the SP/Kruppel or Krueppel-like Factor (KLF) family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. SP factors preferentially bind GC boxes, while KLFs bind CACCC boxes. Another characteristic hallmark of SP factors is the presence of the Buttonhead (BTD) box CXCPXC, just N-terminal to the zinc fingers. The function of the BTD box is unknown, but it is thought to play an important physiological role. Another feature of most SP factors is the presence of a conserved amino acid stretch, the so-called SP box, located close to the N-terminus. SP factors may be separated into three groups based on their domain architecture and the similarity of their N-terminal transactivation domains: SP1-4, SP5, and SP6-9. The transactivation domains between the three groups are not homologous to one another. This model represents the related N-terminal domains of SP6-SP9, and similar proteins.


Pssm-ID: 411692  Cd Length: 162  Bit Score: 148.55  E-value: 1.51e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624   1 MLTAVCGSLGSQHTEAPH--ASPPRLdLQPLQTYQGHtspeagdypsplqpgelqslplgpevdfsqgyelpgassrvtc 78
Cdd:cd22543    1 MLAATCNKLGSQSPLPPSdsAFGKGG-FHPWKRSSSL------------------------------------------- 36

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624  79 edlesdsplapgpfskllqpDMSHHYESWFRPTHPGA---------EDGSWWDLHPGTSWMDLPHtqgaLTSPGhpgalq 149
Cdd:cd22543   37 --------------------DMAHPYESWFKPGHHATiapgevpsnEASSWWDVHPGGSWLDVPH----LLSPG------ 86

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 150 aglggyvgdhqlcappphphahhllpaagGQHLLGPPDGAKALEVAAPESQGLD-SSLDGAARPKGSRRSVPRSSGQTVC 228
Cdd:cd22543   87 -----------------------------GQHLLGQDGYKPVLPGASPESAGSDgSSLPGAASGGGSRRSARRYSGRATC 137

                        250       260
                 ....*....|....*....|....*..
gi 384871624 229 RCPNCLEAERLGAPcgPDGGKKKHLHN 255
Cdd:cd22543  138 DCPNCQEAERLGPA--GAGLRKKGLHS 162
SP8_N cd22538
N-terminal domain of transcription factor Specificity Protein (SP) 8; Specificity Proteins ...
 100-255 2.72e-17

N-terminal domain of transcription factor Specificity Protein (SP) 8; Specificity Proteins (SPs) are transcription factors that are involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. SP8 is crucial for limb outgrowth and neuropore closure. It is expressed during embryogenesis in the forming apical ectodermal ridge, restricted regions of the central nervous system, and tail bud. SP8 and SP9 are two closely related transcription factors that mediate FGF10 signaling, which in turn regulates FGF8 expression which is essential for normal limb development. Both SP8 and SP9 have been found in vertebrates, but only SP8 is present in invertebrates. SP8 belongs to a family of proteins, called the SP/Kruppel or Krueppel-like Factor (KLF) family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. SP factors preferentially bind GC boxes, while KLFs bind CACCC boxes. Another characteristic hallmark of SP factors is the presence of the Buttonhead (BTD) box CXCPXC, just N-terminal to the zinc fingers. The function of the BTD box is unknown, but it is thought to play an important physiological role. Another feature of most SP factors is the presence of a conserved amino acid stretch, the so-called SP box, located close to the N-terminus. SP factors may be separated into three groups based on their domain architecture and the similarity of their N-terminal transactivation domains: SP1-4, SP5, and SP6-9. The transactivation domains between the three groups are not homologous to one another. This model represents the N-terminal domain of SP8.


Pssm-ID: 411690 [Multi-domain]  Cd Length: 303  Bit Score: 81.55  E-value: 2.72e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 100 MSHHYESWFRPTHPGAEDG-------SWWDLHPGtsWMDLPHTQG-ALTSPGHPGALQAG----LGGYVGDHQLCAPPPH 167
Cdd:cd22538  133 MAHPYESWFKPSHPGIATGeggggasSWWDVGAG--WIDVQNPNGaALQTSLHSGGLQTSlhspLGGYNSDYSGLGHSAF 210

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 168 PHAHHLLPAAGGQHLLgppDGAKAL-------EVAAPESQGLDSSLDG---AARPKGSRRSVPRSSGQTVCRCPNCLEAE 237
Cdd:cd22538  211 STGASSHLLTTGQHLM---DGFKPVlppsypdSSPSPLAGAGGSMLTGgptAPLGGSPRSSARRYSGRATCDCPNCQEAE 287

                        170
                 ....*....|....*...
gi 384871624 238 RLGaPCGPdGGKKKHLHN 255
Cdd:cd22538  288 RLG-PAGA-SLRRKGLHS 303
SP9_N cd22549
N-terminal domain of transcription factor Specificity Protein (SP) 9 and similar proteins; ...
 100-255 2.72e-15

N-terminal domain of transcription factor Specificity Protein (SP) 9 and similar proteins; Specificity Proteins (SPs) are transcription factors that are involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. SP9 plays a role in limb outgrowth. It is expressed during embryogenesis in the forming apical ectodermal ridge, restricted regions of the central nervous system, and tail bud. SP8 and SP9 are two closely related transcription factors that mediate FGF10 signaling, which in turn regulates FGF8 expression which is essential for normal limb development. Both SP8 and SP9 have been found in vertebrates, but only SP8 is present in invertebrates. SP9 belongs to a family of proteins, called the SP/Kruppel or Krueppel-like Factor (KLF) family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. SP factors preferentially bind GC boxes, while KLFs bind CACCC boxes. Another characteristic hallmark of SP factors is the presence of the Buttonhead (BTD) box CXCPXC, just N-terminal to the zinc fingers. The function of the BTD box is unknown, but it is thought to play an important physiological role. Another feature of most SP factors is the presence of a conserved amino acid stretch, the so-called SP box, located close to the N-terminus. SP factors may be separated into three groups based on their domain architecture and the similarity of their N-terminal transactivation domains: SP1-4, SP5, and SP6-9. The transactivation domains between the three groups are not homologous to one another. This model represents the N-terminal domain of SP9.


Pssm-ID: 411695 [Multi-domain]  Cd Length: 299  Bit Score: 75.79  E-value: 2.72e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 100 MSHHYESWFR-------PTHPGAEDGSWWDLHPG-TSWMDLPHTQGALTSPGHPGALQAG----LGGYVGDHQLCAPPPH 167
Cdd:cd22549  125 MAHPYESWYKsgfhstiSGDVSGGASSWWDVHTNpSSWLEVQNPAGGLQSSLHSGTPQASlhsqLGGYNPDFSSLTHSAF 204

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 168 PHAHHLLPAA----GGQHLLGPpDGAKAL-----EVAAPESQGLDSSLDGAARPKG-SRRSVPRSSGQTVCRCPNCLEAE 237
Cdd:cd22549  205 SSTGISATAShllsTSQHLLTQ-EGFKPVlpsytDSSAANAMGSASIISGAATLGGgSARSARRYSGRATCDCPNCQEAE 283

                        170       180
                 ....*....|....*....|
gi 384871624 238 RLgapcGPDGG--KKKHLHN 255
Cdd:cd22549  284 RL----GPAGAslRRKGLHS 299
SP7_N cd22542
N-terminal domain of transcription factor Specificity Protein (SP) 7; Specificity Proteins ...
 99-254 2.77e-12

N-terminal domain of transcription factor Specificity Protein (SP) 7; Specificity Proteins (SPs) are transcription factors that are involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. SP7, also called Osterix (Osx) in humans, is highly conserved among bone-forming vertebrates. It plays a major role, along with Runx2 and Dlx5 in driving the differentiation of mesenchymal precursor cells into osteoblasts and eventually osteocytes. SP7 also plays a regulatory role by inhibiting chondrocyte differentiation, maintaining the balance between differentiation of mesenchymal precursor cells into ossified bone or cartilage. Mutations of this gene have been associated with multiple dysfunctional bone phenotypes in vertebrates. SP7 is thought to play a role in diseases such as Osteogenesis imperfecta. SP7 belongs to a family of proteins, called the SP/Kruppel or Krueppel-like Factor (KLF) family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. SP factors preferentially bind GC boxes, while KLFs bind CACCC boxes. Another characteristic hallmark of SP factors is the presence of the Buttonhead (BTD) box CXCPXC, just N-terminal to the zinc fingers. The function of the BTD box is unknown, but it is thought to play an important physiological role. Another feature of most SP factors is the presence of a conserved amino acid stretch, the so-called SP box, located close to the N-terminus. SP factors may be separated into three groups based on their domain architecture and the similarity of their N-terminal transactivation domains: SP1-4, SP5, and SP6-9. The transactivation domains between the three groups are not homologous to one another. This model represents the N-terminal domain of SP7.


Pssm-ID: 411691 [Multi-domain]  Cd Length: 297  Bit Score: 66.85  E-value: 2.77e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624  99 DMSHHYESWFRP-THPGAEDG------SWWDLHPGTSWMDLPHTQGALTSPGHPGALQAGLggyvgDHQLCAPPPHPHAH 171
Cdd:cd22542  136 DMAHPYGSWYKTgIHPGISSSstnataSWWDMHSNTNWLSAQGQPDGLQASLQPVPAQTPL-----NPQLPSYTEFTTLN 210

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 172 HLLPAAGG----QHLLGPPDGAKALEVAAPESQGLDSSLDGAARPKGSRRSVPRS-SGQTVCRCPNCLEAERLGAPCGpd 246
Cdd:cd22542  211 PAPYPAVGisssSHLLPSSQHMLSQDMYKPKPVANNGLMEGGIGLKSPSGGSYGStTGRSSCDCPNCQELERLGASAA-- 288


                 ....*...
gi 384871624 247 GGKKKHLH 254
Cdd:cd22542  289 SLRKKPIH 296
SP6-9-like_N cd22547
N-terminal domain of invertebrate transcription factor Specificity Proteins (SP) similar to ...
 101-241 2.88e-09

N-terminal domain of invertebrate transcription factor Specificity Proteins (SP) similar to SP6, SP8 and SP9; Specificity Proteins (SPs) are transcription factors that are involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. SP6, also known as epiprofin, shows specific expression pattern in hair follicles and the apical ectodermal ridge (AER) of the developing limbs. SP6 null mice are nude and show defects in skin, teeth, limbs (syndactyly and oligodactyly), and lung alveoli. SP9 plays a role in limb outgrowth. It is expressed during embryogenesis in the forming AER, restricted regions of the central nervous system, and tail bud. SP8 and SP9 are two closely related transcription factors that mediate FGF10 signaling, which in turn regulates FGF8 expression which is essential for normal limb development. Both SP8 and SP9 have been found in vertebrates, but only SP8 is present in invertebrates. SPs belong to a family of proteins, called the SP/Kruppel or Krueppel-like Factor (KLF) family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. SP factors preferentially bind GC boxes, while KLFs bind CACCC boxes. Another characteristic hallmark of SP factors is the presence of the Buttonhead (BTD) box CXCPXC, just N-terminal to the zinc fingers. The function of the BTD box is unknown, but it is thought to play an important physiological role. Another feature of most SP factors is the presence of a conserved amino acid stretch, the so-called SP box, located close to the N-terminus. SP factors may be separated into three groups based on their domain architecture and the similarity of their N-terminal transactivation domains: SP1-4, SP5, and SP6-9. The transactivation domains between the three groups are not homologous to one another. This model represents the N-terminal domain of invertebrate SPs similar to SP6, SP8, and SP9.


Pssm-ID: 411694  Cd Length: 219  Bit Score: 56.65  E-value: 2.88e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 101 SHHYESWF-----RPTHPGAED------GSWWDLHP-GTSWMDlphtQGALTSPGHPGALQAGLGgyvgdHQLCAPPPHP 168
Cdd:cd22547   62 HHPYESWPfnatsHHHKKEEVSssannsSSWWDMHSaAGSWLD----ESSAAATGPHSQISPNYP-----SSDYSLGHLL 132

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 384871624 169 HAHHLLPAAGGQHLLGP------PDGAKALEVAAPESQGLDSSLDGAARPKGSRRSvprsSGQTVCRCPNCLEAERLGA 241
Cdd:cd22547  133 ASSSAPLLLSGQHLLQDtyksmlPSQGDIGASSFPSSLLSQPSLSGVPSPRSQRRY----TGRATCDCPNCQEAERLGP 207
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
261-336 4.67e-05

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 45.07  E-value: 4.67e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871624 261 CGKAYAKTSHLKAHLRW--HSG--DRPFVCNWLFCGKRFTRSDELQRHLQTHTGTKKFPCavCSRVFMRSDHLAKHMKTH 336
Cdd:COG5048  295 CNISFSRSSPLTRHLRSvnHSGesLKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKE--KLLNSSSKFSPLLNNEPP 372
zf-H2C2_2 pfam13465
Zinc-finger double domain;
300-323 3.15e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 37.74  E-value: 3.15e-04
                          10        20
                  ....*....|....*....|....
gi 384871624  300 ELQRHLQTHTGTKKFPCAVCSRVF 323
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSF 24
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 314-336 1.46e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 35.74  E-value: 1.46e-03
                          10        20
                  ....*....|....*....|...
gi 384871624  314 FPCAVCSRVFMRSDHLAKHMKTH 336
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 284-308 2.18e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 35.35  E-value: 2.18e-03
                          10        20
                  ....*....|....*....|....*
gi 384871624  284 FVCNwlFCGKRFTRSDELQRHLQTH 308
Cdd:pfam00096   1 YKCP--DCGKSFSRKSNLKRHLRTH 23
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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