SPRY domain-containing protein 7; This family contains SPRY domain-containing protein 7 (also known as SPRY domain-containing protein 7 or CLL deletion region gene 6 protein homolog or CLLD6 or chronic lymphocytic leukemia deletion region gene 6 protein homolog). In humans, CLLD6 is highly expressed in heart, skeletal muscle, and testis as well as cancer cell lines. It also has cross-species conservation, suggesting that it is likely to carry out important cellular processes.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62858419  34 MGTDVVIVKNGRRICGTGACLANAPLHQNKSYFEFKVQSTGIWGIGVATQKANLNQIPLGRDVHSLVMRNDGAIYYNNEE 113
Cdd:cd12880   1 MGPDVVIVKNGRRICGTGAALANAPIVQDKAYFEVKIQSTGVWGVGLATRKTDLNRVPLGNDAESWVLRSDGTIWHNGEV 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62858419 114 KNRLpaNSLPQEGDVVGITYDHVELNIYLNGKNMHCPASGIRGTVYPVVYVDDSAILDCQFSEFYHTPPAGFEKILFEQQ 193
Cdd:cd12880  81 IHKL--KQLVEEGDVIGVTYDHVELNFYLNGKPLDCPITGIKGTVYPVVYVDDGAILDVQFSNFYHPPPSGFEEIMFEQS 158

                ..
gi 62858419 194 IF 195
Cdd:cd12880 159 LL 160

SPRY domain-containing protein 7; This family contains SPRY domain-containing protein 7 (also known as SPRY domain-containing protein 7 or CLL deletion region gene 6 protein homolog or CLLD6 or chronic lymphocytic leukemia deletion region gene 6 protein homolog). In humans, CLLD6 is highly expressed in heart, skeletal muscle, and testis as well as cancer cell lines. It also has cross-species conservation, suggesting that it is likely to carry out important cellular processes.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62858419  34 MGTDVVIVKNGRRICGTGACLANAPLHQNKSYFEFKVQSTGIWGIGVATQKANLNQIPLGRDVHSLVMRNDGAIYYNNEE 113
Cdd:cd12880   1 MGPDVVIVKNGRRICGTGAALANAPIVQDKAYFEVKIQSTGVWGVGLATRKTDLNRVPLGNDAESWVLRSDGTIWHNGEV 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62858419 114 KNRLpaNSLPQEGDVVGITYDHVELNIYLNGKNMHCPASGIRGTVYPVVYVDDSAILDCQFSEFYHTPPAGFEKILFEQQ 193
Cdd:cd12880  81 IHKL--KQLVEEGDVIGVTYDHVELNFYLNGKPLDCPITGIKGTVYPVVYVDDGAILDVQFSNFYHPPPSGFEEIMFEQS 158

                ..
gi 62858419 194 IF 195
Cdd:cd12880 159 LL 160

SPRY domain; SPRY Domain is named from SPla and the RYanodine Receptor and it is found in many eukaryotic proteins with a wide range of functions. It is a protein-interaction module involved in many important signalling pathways like RNA processing, regulation of histone H3 methylation, innate immunity or embryonic development. It can be divided into 11 subfamilies based on amino acid sequence similarity or the presence of additional protein domains. The greater SPRY family is divided into the SPRY/B30.2 (which contains a PRY extension at the N-terminal) and SPRY-only sub-families which are preceded by a subdomain that is structurally similar to the PRY region. SPRY/B30.2 structures revealed a bent beta-sandwich fold comprised of two beta-sheets. Distant homologs are domains in butyrophilin/ marenostrin/pyrin.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62858419    63 KSYFEFKV--QSTGIWGIGVATQKANL-NQIPLGRDVHSLVMRNDGAIYYNNEEKNRLPANSLpQEGDVVGITYDHV--E 137
Cdd:pfam00622   1 RHYFEVEIfgQDGGGWRVGWATKSVPRkGERFLGDESGSWGYDGWTGKKYWASTSPLTGLPLF-EPGDVIGCFLDYEagT 79

                          90       100
                  ....*....|....*....|....*....
gi 62858419   138 LNIYLNGKNMHCPASGIR--GTVYPVVYV 164
Cdd:pfam00622  80 ISFTKNGKSLGYAFRDVPfaGPLFPAVSL 108

SPRY domain-containing protein 7; This family contains SPRY domain-containing protein 7 (also known as SPRY domain-containing protein 7 or CLL deletion region gene 6 protein homolog or CLLD6 or chronic lymphocytic leukemia deletion region gene 6 protein homolog). In humans, CLLD6 is highly expressed in heart, skeletal muscle, and testis as well as cancer cell lines. It also has cross-species conservation, suggesting that it is likely to carry out important cellular processes.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62858419  34 MGTDVVIVKNGRRICGTGACLANAPLHQNKSYFEFKVQSTGIWGIGVATQKANLNQIPLGRDVHSLVMRNDGAIYYNNEE 113
Cdd:cd12880   1 MGPDVVIVKNGRRICGTGAALANAPIVQDKAYFEVKIQSTGVWGVGLATRKTDLNRVPLGNDAESWVLRSDGTIWHNGEV 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62858419 114 KNRLpaNSLPQEGDVVGITYDHVELNIYLNGKNMHCPASGIRGTVYPVVYVDDSAILDCQFSEFYHTPPAGFEKILFEQQ 193
Cdd:cd12880  81 IHKL--KQLVEEGDVIGVTYDHVELNFYLNGKPLDCPITGIKGTVYPVVYVDDGAILDVQFSNFYHPPPSGFEEIMFEQS 158

                ..
gi 62858419 194 IF 195
Cdd:cd12880 159 LL 160

SPRY domain; SPRY domains, first identified in the SP1A kinase of Dictyostelium and rabbit Ryanodine receptor (hence the name), are homologous to B30.2. SPRY domains have been identified in at least 11 protein families, covering a wide range of functions, including regulation of cytokine signaling (SOCS), RNA metabolism (DDX1 and hnRNP), immunity to retroviruses (TRIM5alpha), intracellular calcium release (ryanodine receptors or RyR) and regulatory and developmental processes (HERC1 and Ash2L). B30.2 also contains residues in the N-terminus that form a distinct PRY domain structure; i.e. B30.2 domain consists of PRY and SPRY subdomains. B30.2 domains comprise the C-terminus of three protein families: BTNs (receptor glycoproteins of immunoglobulin superfamily); several TRIM proteins (composed of RING/B-box/coiled-coil or RBCC core); Stonutoxin (secreted poisonous protein of the stonefish Synanceia horrida). TRIM/RBCC proteins are involved in a variety of processes, including apoptosis, cell cycle regulation, cell growth, senescence, viral response, meiosis, cell differentiation, and vesicular transport. Genes belonging to this family are implicated in several human diseases that vary from cancer to rare genetic syndromes. The PRY-SPRY domain in these TRIM families is suggested to serve as the target binding site. While SPRY domains are evolutionarily ancient, B30.2 domains are a more recent adaptation where the SPRY/PRY combination is a possible component of immune defense. Mutations found in the SPRY-containing proteins have shown to cause Mediterranean fever and Opitz syndrome.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62858419  63 KSYFEFKVQST--GIWGIGVATQKANLN-QIPLGRDVHSLVMrnDGAIYYNNEEKNRLPANSLPQEGDVVGITYD--HVE 137
Cdd:cd11709   2 KWYWEVRVDSGngGLIQVGWATKSFSLDgEGGVGDDEESWGY--DGSRLRKGHGGSSGPGGRPWKSGDVVGCLLDldEGT 79

                        90       100       110
                ....*....|....*....|....*....|....*....
gi 62858419 138 LNIYLNGKNMHCPASGI---RGTVYPVVYVDDSAILDCQ 173
Cdd:cd11709  80 LSFSLNGKDLGVAFTNLflkGGGLYPAVSLGSGQGVTIN 118

SPRY domain in the suppressor of cytokine signaling 3 (SOCS3) family; The SPRY domain-containing SOCS box protein family (SPSB1-4, also known as SSB-1 to -4) is composed of a central SPRY protein interaction domain and a C-terminal SOCS box. All four SPSB proteins interact with c-Met, the hepatocyte growth factor receptor, but SOCS3 regulates cellular response to a variety of cytokines such as leukemia inhibitory factor (LIF) and interleukin 6. SOCS3, along with SOCS1, are expressed by immune cells and cells of the central nervous system (CNS) and have the potential to impact immune processes within the CNS. In non-small cell lung cancer (NSCLC), SOCS3 is silenced and proline-rich tyrosine kinase 2 (Pyk2) is over-expressed; it has been suggested that SOCS3 could be an effective way to prevent the progression of NSCLC due to its role in regulating Pyk2 expression.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62858419  30 DTQHMGTDVVIVKNGRRI-------CGTGACLANAPLHQNKSYFEFKVqSTGIWG----IGVATQKANLNQI------PL 92
Cdd:cd12876   5 DERDKSPAVQLSDNNREVyfhpdysCGTAAVRGTKPLTNGQHYWEIKM-SSPVYGtdmmVGVGTKKADLHAYryefcsLL 83

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 62858419  93 GRDVHSLVMRNDGAIYYNNEEKNRlpANSLPQEGDVVGITYD--HVELNIYLNGKNMHCPASGIRG--TVYPVV 162
Cdd:cd12876  84 GEDEESWGLSYKGLLWHDGQSRPY--TSPFGNQGTIIGVHLDmwRGTLTFYKNGKPLGVAFTGLNGvkPLYPMV 155