NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|62472347|ref|NP_001014600|]
View 

uncharacterized protein Dmel_CG14457, isoform B [Drosophila melanogaster]

Protein Classification

JHBP domain-containing protein( domain architecture ID 10534990)

JHBP (juvenile hormone-binding protein) domain-containing protein similar to Drosophila melanogaster protein takeout that participates in a novel circadian output pathway that conveys temporal and food status information to feeding-relevant metabolisms and activities

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
JHBP pfam06585
Haemolymph juvenile hormone binding protein (JHBP); This family consists of several ...
 8-253 1.58e-67

Haemolymph juvenile hormone binding protein (JHBP); This family consists of several insect-specific haemolymph juvenile hormone binding proteins (JHBP). Juvenile hormone regulates embryogenesis, maintains the status quo of larval development and stimulates reproductive maturation in the adult insect. JH is transported from the sites of its synthesis to target tissues by a haemolymph carrier called juvenile hormone-binding protein (JHBP). JHBP protects the JH molecules from hydrolysis by non-specific esterases present in the insect haemolymph. The crystal structure of the JHBP from Galleria mellonella shows an unusual fold consisting of a long alpha-helix wrapped in a much curved antiparallel beta-sheet. The folding pattern for this structure closely resembles that found in some tandem-repeat mammalian lipid-binding and bactericidal permeability-increasing proteins, with a similar organization of the major cavity and a disulfide bond linking the long helix and the beta-sheet. It would appear that JHBP forms two cavities, only one of which, the one near the N- and C-termini, binds the hormone; binding induces a conformational change, of unknown significance. This family now includes DUF233, pfam03027.


:

Pssm-ID: 461956  Cd Length: 239  Bit Score: 209.37  E-value: 1.58e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347     8 VLLVTFTTCLvraedgFLKEPPDYIKECRIADKDFVNCSTHSIQQLFDKLNDGIPGLtSIRSFDPFYLNRIRITQGNsNA 87
Cdd:pfam06585   1 LLLLLLAVAA------SAAELPSYIKKCKRSDPNLNKCLKEAIEALRPQLAKGIPEL-GIPPLDPLVIDELSIDQGS-GP 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347    88 INLKVELANVKIIGFGHTNVLDSQVFKKDYSWKTTFTLPEMKLQADYSLFGRILLIPLNGKGQVFLDAENMTVTMHTKTR 167
Cdd:pfam06585  73 VGLKLNLKNVKIYGLSNFTIKKVKVDLKDLKIEFDLLFPKLRLEGKYKADGRILLLPINGKGDFNITLENLKAKGTLKGE 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347   168 LYSKGGFTFYNVTNLHVDFKMDGLKSYFSNLFNGNKQLEDSTNKFFNDNWRMLADALYTVITQTIEDILLDVLKKIFHFI 247
Cdd:pfam06585 153 PVEKNGKTYLKITDLKVKFKVGDVKFHLDNLFNGNKELGDAINQFLNENWREVLNELKPAIEEALSEIFTDILNKIFSNV 232


                  ....*.
gi 62472347   248 PANFFV 253
Cdd:pfam06585 233 PYDELF 238
 
Name Accession Description Interval E-value
JHBP pfam06585
Haemolymph juvenile hormone binding protein (JHBP); This family consists of several ...
 8-253 1.58e-67

Haemolymph juvenile hormone binding protein (JHBP); This family consists of several insect-specific haemolymph juvenile hormone binding proteins (JHBP). Juvenile hormone regulates embryogenesis, maintains the status quo of larval development and stimulates reproductive maturation in the adult insect. JH is transported from the sites of its synthesis to target tissues by a haemolymph carrier called juvenile hormone-binding protein (JHBP). JHBP protects the JH molecules from hydrolysis by non-specific esterases present in the insect haemolymph. The crystal structure of the JHBP from Galleria mellonella shows an unusual fold consisting of a long alpha-helix wrapped in a much curved antiparallel beta-sheet. The folding pattern for this structure closely resembles that found in some tandem-repeat mammalian lipid-binding and bactericidal permeability-increasing proteins, with a similar organization of the major cavity and a disulfide bond linking the long helix and the beta-sheet. It would appear that JHBP forms two cavities, only one of which, the one near the N- and C-termini, binds the hormone; binding induces a conformational change, of unknown significance. This family now includes DUF233, pfam03027.


Pssm-ID: 461956  Cd Length: 239  Bit Score: 209.37  E-value: 1.58e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347     8 VLLVTFTTCLvraedgFLKEPPDYIKECRIADKDFVNCSTHSIQQLFDKLNDGIPGLtSIRSFDPFYLNRIRITQGNsNA 87
Cdd:pfam06585   1 LLLLLLAVAA------SAAELPSYIKKCKRSDPNLNKCLKEAIEALRPQLAKGIPEL-GIPPLDPLVIDELSIDQGS-GP 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347    88 INLKVELANVKIIGFGHTNVLDSQVFKKDYSWKTTFTLPEMKLQADYSLFGRILLIPLNGKGQVFLDAENMTVTMHTKTR 167
Cdd:pfam06585  73 VGLKLNLKNVKIYGLSNFTIKKVKVDLKDLKIEFDLLFPKLRLEGKYKADGRILLLPINGKGDFNITLENLKAKGTLKGE 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347   168 LYSKGGFTFYNVTNLHVDFKMDGLKSYFSNLFNGNKQLEDSTNKFFNDNWRMLADALYTVITQTIEDILLDVLKKIFHFI 247
Cdd:pfam06585 153 PVEKNGKTYLKITDLKVKFKVGDVKFHLDNLFNGNKELGDAINQFLNENWREVLNELKPAIEEALSEIFTDILNKIFSNV 232


                  ....*.
gi 62472347   248 PANFFV 253
Cdd:pfam06585 233 PYDELF 238
JHBP smart00700
Juvenile hormone binding protein domains in insects; The juvenile hormone exerts pleiotropic ...
 29-254 6.42e-54

Juvenile hormone binding protein domains in insects; The juvenile hormone exerts pleiotropic functions during insect life cycles and its binding proteins regulate these functions.


Pssm-ID: 214779  Cd Length: 224  Bit Score: 174.01  E-value: 6.42e-54
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347     29 PDYIKECRIadKDFVNCSTHSIQQLFDKLNDGIPGLtSIRSFDPFYLNRIRITQGNSNaINLKVELANVKIIGFGHTNVL 108
Cdd:smart00700   4 PAFLKPCKL--GDPSECLRDAIEALLPQLKNGIPEY-GIPPLDPLEIDDLKISIDSGV-IGLRLTFKNVKIYGLSNFEIT 79

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347    109 DSQVFKKDYSWKTTFTLPEMKLQADYSLFGRILLIPLNGKGQVFLDAENMTVTMHTKTRLYSKGgFTFYNVTNLHVDFKM 188
Cdd:smart00700  80 KFKMDLKDKKIELKIEFPKLNVKGDYKLDGRLLGLPLNGKGDANFTLENVKIRGTLKLKLGPDG-KTYLKIKSLKVNFEV 158

                          170       180       190       200       210       220
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 62472347    189 DGLKSYFSNLFNGNKQLEDSTNKFFNDNWRMLADALYTVITQTIEDILLDVLKKIFHFIPANFFVS 254
Cdd:smart00700 159 GDVKSHLDNLFNGNKDLNDAINKFLNENWKALINELLPAIVEKLESIFLDLVNKVFAKVPIDEFFV 224
 
Name Accession Description Interval E-value
JHBP pfam06585
Haemolymph juvenile hormone binding protein (JHBP); This family consists of several ...
 8-253 1.58e-67

Haemolymph juvenile hormone binding protein (JHBP); This family consists of several insect-specific haemolymph juvenile hormone binding proteins (JHBP). Juvenile hormone regulates embryogenesis, maintains the status quo of larval development and stimulates reproductive maturation in the adult insect. JH is transported from the sites of its synthesis to target tissues by a haemolymph carrier called juvenile hormone-binding protein (JHBP). JHBP protects the JH molecules from hydrolysis by non-specific esterases present in the insect haemolymph. The crystal structure of the JHBP from Galleria mellonella shows an unusual fold consisting of a long alpha-helix wrapped in a much curved antiparallel beta-sheet. The folding pattern for this structure closely resembles that found in some tandem-repeat mammalian lipid-binding and bactericidal permeability-increasing proteins, with a similar organization of the major cavity and a disulfide bond linking the long helix and the beta-sheet. It would appear that JHBP forms two cavities, only one of which, the one near the N- and C-termini, binds the hormone; binding induces a conformational change, of unknown significance. This family now includes DUF233, pfam03027.


Pssm-ID: 461956  Cd Length: 239  Bit Score: 209.37  E-value: 1.58e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347     8 VLLVTFTTCLvraedgFLKEPPDYIKECRIADKDFVNCSTHSIQQLFDKLNDGIPGLtSIRSFDPFYLNRIRITQGNsNA 87
Cdd:pfam06585   1 LLLLLLAVAA------SAAELPSYIKKCKRSDPNLNKCLKEAIEALRPQLAKGIPEL-GIPPLDPLVIDELSIDQGS-GP 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347    88 INLKVELANVKIIGFGHTNVLDSQVFKKDYSWKTTFTLPEMKLQADYSLFGRILLIPLNGKGQVFLDAENMTVTMHTKTR 167
Cdd:pfam06585  73 VGLKLNLKNVKIYGLSNFTIKKVKVDLKDLKIEFDLLFPKLRLEGKYKADGRILLLPINGKGDFNITLENLKAKGTLKGE 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347   168 LYSKGGFTFYNVTNLHVDFKMDGLKSYFSNLFNGNKQLEDSTNKFFNDNWRMLADALYTVITQTIEDILLDVLKKIFHFI 247
Cdd:pfam06585 153 PVEKNGKTYLKITDLKVKFKVGDVKFHLDNLFNGNKELGDAINQFLNENWREVLNELKPAIEEALSEIFTDILNKIFSNV 232


                  ....*.
gi 62472347   248 PANFFV 253
Cdd:pfam06585 233 PYDELF 238
JHBP smart00700
Juvenile hormone binding protein domains in insects; The juvenile hormone exerts pleiotropic ...
 29-254 6.42e-54

Juvenile hormone binding protein domains in insects; The juvenile hormone exerts pleiotropic functions during insect life cycles and its binding proteins regulate these functions.


Pssm-ID: 214779  Cd Length: 224  Bit Score: 174.01  E-value: 6.42e-54
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347     29 PDYIKECRIadKDFVNCSTHSIQQLFDKLNDGIPGLtSIRSFDPFYLNRIRITQGNSNaINLKVELANVKIIGFGHTNVL 108
Cdd:smart00700   4 PAFLKPCKL--GDPSECLRDAIEALLPQLKNGIPEY-GIPPLDPLEIDDLKISIDSGV-IGLRLTFKNVKIYGLSNFEIT 79

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62472347    109 DSQVFKKDYSWKTTFTLPEMKLQADYSLFGRILLIPLNGKGQVFLDAENMTVTMHTKTRLYSKGgFTFYNVTNLHVDFKM 188
Cdd:smart00700  80 KFKMDLKDKKIELKIEFPKLNVKGDYKLDGRLLGLPLNGKGDANFTLENVKIRGTLKLKLGPDG-KTYLKIKSLKVNFEV 158

                          170       180       190       200       210       220
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 62472347    189 DGLKSYFSNLFNGNKQLEDSTNKFFNDNWRMLADALYTVITQTIEDILLDVLKKIFHFIPANFFVS 254
Cdd:smart00700 159 GDVKSHLDNLFNGNKDLNDAINKFLNENWKALINELLPAIVEKLESIFLDLVNKVFAKVPIDEFFV 224
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH