NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|973165470|gb|KUL05243|]
View 

Putative methylthioribose-1-phosphate isomerase [Methanomicrobiales archaeon 53_19]

Protein Classification

S-methyl-5-thioribose-1-phosphate isomerase( domain architecture ID 10000693)

S-methyl-5-thioribose-1-phosphate isomerase catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) and methylthioribulose-1-phosphate (MTRu-1-P)

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
MtnA COG0182
5-methylthioribose/5-deoxyribulose 1-phosphate isomerase (methionine salvage pathway), a ...
 5-333 0e+00

5-methylthioribose/5-deoxyribulose 1-phosphate isomerase (methionine salvage pathway), a paralog of eIF-2B alpha subunit [Amino acid transport and metabolism];


:

Pssm-ID: 439952  Cd Length: 343  Bit Score: 514.97  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   5 RTIWWEEDGVGLIEQTLLPEEYREVVCSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAAS 84
Cdd:COG0182    4 RTIWWDGGALVLLDQTLLPHEEEYVELTTVEDVAEAIRDMVVRGAPAIGVAAAYGVALAAREAAADDREEFLAELEEAAE 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  85 TLRSARPTAVNLAWGVDRVLSAIQPVSDPVDAAERALTMAESIADEDEATCRRIGSHGAPLLPDDCTVLTHCNAGALACA 164
Cdd:COG0182   84 YLAATRPTAVNLFWALDRMLAALEELPSVEEIREALLAEALAIADEDVAANRAIGEHGAELIPDGDTILTHCNAGALATV 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 165 AWGTALGVIRSAAEEGKNIRVISCETRPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADRICTDG-V 243
Cdd:COG0182  164 GYGTALGVIRAAHEAGKLIHVYADETRPLLQGARLTAWELMQDGIPVTLITDNAAGHLMQRGKVDAVIVGADRIAANGdV 243

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 244 FNKIGTYMHAVSASYHQIPFYVAAPRSTFDPE-HRMADIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPFSLIAAI 322
Cdd:COG0182  244 ANKIGTYGLAVLAKHHGIPFYVAAPTSTIDLSlPDGEDIPIEERDPDEVTHVGGRRIAPEGVPVYNPAFDVTPAELITAI 323

                        330
                 ....*....|.
gi 973165470 323 ITEDGLIREPF 333
Cdd:COG0182  324 ITEKGVIRPPY 334
 
Name Accession Description Interval E-value
MtnA COG0182
5-methylthioribose/5-deoxyribulose 1-phosphate isomerase (methionine salvage pathway), a ...
 5-333 0e+00

5-methylthioribose/5-deoxyribulose 1-phosphate isomerase (methionine salvage pathway), a paralog of eIF-2B alpha subunit [Amino acid transport and metabolism];


Pssm-ID: 439952  Cd Length: 343  Bit Score: 514.97  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   5 RTIWWEEDGVGLIEQTLLPEEYREVVCSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAAS 84
Cdd:COG0182    4 RTIWWDGGALVLLDQTLLPHEEEYVELTTVEDVAEAIRDMVVRGAPAIGVAAAYGVALAAREAAADDREEFLAELEEAAE 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  85 TLRSARPTAVNLAWGVDRVLSAIQPVSDPVDAAERALTMAESIADEDEATCRRIGSHGAPLLPDDCTVLTHCNAGALACA 164
Cdd:COG0182   84 YLAATRPTAVNLFWALDRMLAALEELPSVEEIREALLAEALAIADEDVAANRAIGEHGAELIPDGDTILTHCNAGALATV 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 165 AWGTALGVIRSAAEEGKNIRVISCETRPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADRICTDG-V 243
Cdd:COG0182  164 GYGTALGVIRAAHEAGKLIHVYADETRPLLQGARLTAWELMQDGIPVTLITDNAAGHLMQRGKVDAVIVGADRIAANGdV 243

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 244 FNKIGTYMHAVSASYHQIPFYVAAPRSTFDPE-HRMADIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPFSLIAAI 322
Cdd:COG0182  244 ANKIGTYGLAVLAKHHGIPFYVAAPTSTIDLSlPDGEDIPIEERDPDEVTHVGGRRIAPEGVPVYNPAFDVTPAELITAI 323

                        330
                 ....*....|.
gi 973165470 323 ITEDGLIREPF 333
Cdd:COG0182  324 ITEKGVIRPPY 334
PRK06036 PRK06036
S-methyl-5-thioribose-1-phosphate isomerase;
5-333 9.63e-159

S-methyl-5-thioribose-1-phosphate isomerase;


Pssm-ID: 180362  Cd Length: 339  Bit Score: 447.63  E-value: 9.63e-159
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   5 RTIWW--EEDGVGLIEQTLLPEEYREVVCSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEA 82
Cdd:PRK06036   2 RTIDWndESNSVKLIDQTLLPEEYKVIECKTLESLCEAIKSLRVRGAPALGAAGGYGIALAARLSKAKDVDELLKDLKVA 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  83 ASTLRSARPTAVNLAWGVDRVLSAIQPVSDPVDAAERALTMAESIADEDEATCRRIGSHGAPLLPDDCTVLTHCNAGALA 162
Cdd:PRK06036  82 AETLKSTRPTAVNLSWGVDRVLKAALDAEDVEEIRDIALREAERIAEEDVARNKLIGKHGAKLLEDGDTVLTHCNAGRLA 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 163 CAAWGTALGVIRSAAEEGKNIRVISCETRPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADRICTDG 242
Cdd:PRK06036 162 CVDWGTALGVIRSAVEQGKEIKVIACETRPLNQGSRLTTWELMQDNIPVTLITDSMAGIVMRQGMVDKVIVGADRITRDA 241

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 243 VFNKIGTYMHAVSASYHQIPFYVAAPRSTFDPEHRMADIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPFSLIAAI 322
Cdd:PRK06036 242 VFNKIGTYTHSVLAKEHEIPFYVAAPLSTFDFEGWEGSVKIEERDPDELRYCGKTQIAPKDVPVYNPAFDATPMENVTAI 321

                        330
                 ....*....|.
gi 973165470 323 ITEDGLIREPF 333
Cdd:PRK06036 322 ITEKGVFYPPF 332
salvage_mtnA TIGR00512
S-methyl-5-thioribose-1-phosphate isomerase; The delineation of this family was based in part ...
 5-330 1.92e-139

S-methyl-5-thioribose-1-phosphate isomerase; The delineation of this family was based in part on a discussion and neighbor-joining phylogenetic study, by Kyrpides and Woese, of archaeal and other proteins homologous to the alpha, beta, and delta subunits of eukaryotic initiation factor 2B (eIF-2B), a five-subunit molecule that catalyzes GTP recycling for eIF-2. This clade is now recognized to include the methionine salvage pathway enzyme MtnA. [Amino acid biosynthesis, Aspartate family]


Pssm-ID: 273112 [Multi-domain]  Cd Length: 335  Bit Score: 398.55  E-value: 1.92e-139
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470    5 RTIWWEEDGVGLIEQTLLPEEYREVVCSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAAS 84
Cdd:TIGR00512   1 RAIKWSRGSLEILDQRLLPHESEYIEVTTVEDVADAIRDMRVRGAPAIGIVAAYGLALAAREGETDEGEEFKAFLEEKLQ 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   85 TLRSARPTAVNLAWGVDRVLSAIQPVSDPVDAAERALTMAESIADEDEATCRRIGSHGAPLLPD------DCTVLTHCNA 158
Cdd:TIGR00512  81 YLVSSRPTAVNLSWALDRMRAALREAKTVADIKEALLAEAERILEEDLEDNRAIGENGAALIKKgvaakePVRVLTHCNT 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  159 GALACAAWGTALGVIRSAAEEGKNIRVISCETRPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADRI 238
Cdd:TIGR00512 161 GSLATAGYGTALGVIRSAHEKGRLEHVYADETRPYNQGARLTAWELVQEGIPATLITDSMAAHLMKHGEVDAVIVGADRI 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  239 CTDG-VFNKIGTYMHAVSASYHQIPFYVAAPRSTFDPE-HRMADIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPF 316
Cdd:TIGR00512 241 AANGdTANKIGTYQLAILAKHHGVPFYVAAPTSTIDLElADGAEIPIEERPPEEVTHVGGVRIAPPGIGVWNPAFDVTPA 320

                         330
                  ....*....|....
gi 973165470  317 SLIAAIITEDGLIR 330
Cdd:TIGR00512 321 ELITGIITEKGVIT 334
IF-2B pfam01008
Initiation factor 2 subunit family; This family includes initiation factor 2B alpha, beta and ...
 45-329 3.88e-92

Initiation factor 2 subunit family; This family includes initiation factor 2B alpha, beta and delta subunits from eukaryotes, initiation factor 2B subunits 1 and 2 from archaebacteria and some proteins of unknown function from prokaryotes. Initiation factor 2 binds to Met-tRNA, GTP and the small ribosomal subunit. Members of this family have also been characterized as 5-methylthioribose- 1-phosphate isomerases, an enzyme of the methionine salvage pathway. The crystal structure of Ypr118w, a non-essential, low-copy number gene product from Saccharomyces cerevisiae, reveals a dimeric protein with two domains and a putative active site cleft.


Pssm-ID: 395798 [Multi-domain]  Cd Length: 281  Bit Score: 276.10  E-value: 3.88e-92
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   45 EVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAASTLRSARPTAVNLAWGVDRVL---SAIQPVSDPVDAAERAL 121
Cdd:pfam01008   1 KVRGSPAIAIAALEGLLLVIQDARTPTVAELKEQLRSAIEFLISARPTAVSLGNAIDRLLrivLALHSSSDVEEAKESLI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  122 TMAESIADEDEATCRRIGSHGAPLLPDDCTVLTHCNAgalacaawGTALGVIRSAAEEGKNIRVISCETRPLLQGsRLTA 201
Cdd:pfam01008  81 EAADEFIDEIEEARRKIGAIAAELIKDGDTILTHCNS--------GTVLGVLRAAHKEGKRFRVIVTESRPRLQG-RLTA 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  202 WELSRDGIPVTTIIDSEAAYLMQRgeIDCVIVGADRICTDG-VFNKIGTYMHAVSASYHQIPFYVAAPRSTFDPEHRM-A 279
Cdd:pfam01008 152 KELVQAGIPVTLITDSAVGYVMQE--VDKVIVGADRILANGgIANKIGTYQLALLAKAHNVPFYVVAETYKFDPRFPLdE 229

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|
gi 973165470  280 DIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPFSLIAAIITEDGLI 329
Cdd:pfam01008 230 DIFIEERDPEEVLYRTGVRIAPPNLKVRNPAFDYTPPELITLIITEVGVL 279
 
Name Accession Description Interval E-value
MtnA COG0182
5-methylthioribose/5-deoxyribulose 1-phosphate isomerase (methionine salvage pathway), a ...
 5-333 0e+00

5-methylthioribose/5-deoxyribulose 1-phosphate isomerase (methionine salvage pathway), a paralog of eIF-2B alpha subunit [Amino acid transport and metabolism];


Pssm-ID: 439952  Cd Length: 343  Bit Score: 514.97  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   5 RTIWWEEDGVGLIEQTLLPEEYREVVCSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAAS 84
Cdd:COG0182    4 RTIWWDGGALVLLDQTLLPHEEEYVELTTVEDVAEAIRDMVVRGAPAIGVAAAYGVALAAREAAADDREEFLAELEEAAE 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  85 TLRSARPTAVNLAWGVDRVLSAIQPVSDPVDAAERALTMAESIADEDEATCRRIGSHGAPLLPDDCTVLTHCNAGALACA 164
Cdd:COG0182   84 YLAATRPTAVNLFWALDRMLAALEELPSVEEIREALLAEALAIADEDVAANRAIGEHGAELIPDGDTILTHCNAGALATV 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 165 AWGTALGVIRSAAEEGKNIRVISCETRPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADRICTDG-V 243
Cdd:COG0182  164 GYGTALGVIRAAHEAGKLIHVYADETRPLLQGARLTAWELMQDGIPVTLITDNAAGHLMQRGKVDAVIVGADRIAANGdV 243

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 244 FNKIGTYMHAVSASYHQIPFYVAAPRSTFDPE-HRMADIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPFSLIAAI 322
Cdd:COG0182  244 ANKIGTYGLAVLAKHHGIPFYVAAPTSTIDLSlPDGEDIPIEERDPDEVTHVGGRRIAPEGVPVYNPAFDVTPAELITAI 323

                        330
                 ....*....|.
gi 973165470 323 ITEDGLIREPF 333
Cdd:COG0182  324 ITEKGVIRPPY 334
PRK06036 PRK06036
S-methyl-5-thioribose-1-phosphate isomerase;
5-333 9.63e-159

S-methyl-5-thioribose-1-phosphate isomerase;


Pssm-ID: 180362  Cd Length: 339  Bit Score: 447.63  E-value: 9.63e-159
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   5 RTIWW--EEDGVGLIEQTLLPEEYREVVCSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEA 82
Cdd:PRK06036   2 RTIDWndESNSVKLIDQTLLPEEYKVIECKTLESLCEAIKSLRVRGAPALGAAGGYGIALAARLSKAKDVDELLKDLKVA 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  83 ASTLRSARPTAVNLAWGVDRVLSAIQPVSDPVDAAERALTMAESIADEDEATCRRIGSHGAPLLPDDCTVLTHCNAGALA 162
Cdd:PRK06036  82 AETLKSTRPTAVNLSWGVDRVLKAALDAEDVEEIRDIALREAERIAEEDVARNKLIGKHGAKLLEDGDTVLTHCNAGRLA 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 163 CAAWGTALGVIRSAAEEGKNIRVISCETRPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADRICTDG 242
Cdd:PRK06036 162 CVDWGTALGVIRSAVEQGKEIKVIACETRPLNQGSRLTTWELMQDNIPVTLITDSMAGIVMRQGMVDKVIVGADRITRDA 241

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 243 VFNKIGTYMHAVSASYHQIPFYVAAPRSTFDPEHRMADIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPFSLIAAI 322
Cdd:PRK06036 242 VFNKIGTYTHSVLAKEHEIPFYVAAPLSTFDFEGWEGSVKIEERDPDELRYCGKTQIAPKDVPVYNPAFDATPMENVTAI 321

                        330
                 ....*....|.
gi 973165470 323 ITEDGLIREPF 333
Cdd:PRK06036 322 ITEKGVFYPPF 332
mtnA PRK05720
methylthioribose-1-phosphate isomerase; Reviewed
 5-333 2.06e-143

methylthioribose-1-phosphate isomerase; Reviewed


Pssm-ID: 235578  Cd Length: 344  Bit Score: 408.82  E-value: 2.06e-143
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   5 RTIWWEEDGVGLIEQTLLPEEYREVVCSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAAS 84
Cdd:PRK05720   4 RPVAWKDGAVRILDQRKLPHEVEYVELTTAEEVADAIRDMVVRGAPAIGIAAAYGMALAAREDASDDGEEFLKKLEEAAA 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  85 TLRSARPTAVNLAWGVDRVLSAIQPVSdPVDAAERALTMAESIADEDEATCRRIGSHGAPLLPDDCTVLTHCNAGALACA 164
Cdd:PRK05720  84 YLAASRPTAVNLFWALDRMREVLAPLP-GAERKAALEEEAIEIHEEDVEINRAIGEHGLTLIRKGQGILTHCNAGWLATA 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 165 AWGTALGVIRSAAEEGKNIRVISCETRPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADRICTDG-V 243
Cdd:PRK05720 163 GYGTALAPIYAAKEKGIDIHVYADETRPRLQGARLTAWELYQAGIDVTVITDNMAAHLMQTGKIDAVIVGADRIAANGdV 242

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 244 FNKIGTYMHAVSASYHQIPFYVAAPRSTFDPE-HRMADIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPFSLIAAI 322
Cdd:PRK05720 243 ANKIGTYQLAIAAKYHGVPFYVAAPSSTIDLTlADGKEIPIEERDPEEVTEVGGVRIAPEGVKVYNPAFDVTPAELITGI 322

                        330
                 ....*....|.
gi 973165470 323 ITEDGLIREPF 333
Cdd:PRK05720 323 ITEKGIVAPPD 333
salvage_mtnA TIGR00512
S-methyl-5-thioribose-1-phosphate isomerase; The delineation of this family was based in part ...
 5-330 1.92e-139

S-methyl-5-thioribose-1-phosphate isomerase; The delineation of this family was based in part on a discussion and neighbor-joining phylogenetic study, by Kyrpides and Woese, of archaeal and other proteins homologous to the alpha, beta, and delta subunits of eukaryotic initiation factor 2B (eIF-2B), a five-subunit molecule that catalyzes GTP recycling for eIF-2. This clade is now recognized to include the methionine salvage pathway enzyme MtnA. [Amino acid biosynthesis, Aspartate family]


Pssm-ID: 273112 [Multi-domain]  Cd Length: 335  Bit Score: 398.55  E-value: 1.92e-139
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470    5 RTIWWEEDGVGLIEQTLLPEEYREVVCSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAAS 84
Cdd:TIGR00512   1 RAIKWSRGSLEILDQRLLPHESEYIEVTTVEDVADAIRDMRVRGAPAIGIVAAYGLALAAREGETDEGEEFKAFLEEKLQ 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   85 TLRSARPTAVNLAWGVDRVLSAIQPVSDPVDAAERALTMAESIADEDEATCRRIGSHGAPLLPD------DCTVLTHCNA 158
Cdd:TIGR00512  81 YLVSSRPTAVNLSWALDRMRAALREAKTVADIKEALLAEAERILEEDLEDNRAIGENGAALIKKgvaakePVRVLTHCNT 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  159 GALACAAWGTALGVIRSAAEEGKNIRVISCETRPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADRI 238
Cdd:TIGR00512 161 GSLATAGYGTALGVIRSAHEKGRLEHVYADETRPYNQGARLTAWELVQEGIPATLITDSMAAHLMKHGEVDAVIVGADRI 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  239 CTDG-VFNKIGTYMHAVSASYHQIPFYVAAPRSTFDPE-HRMADIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPF 316
Cdd:TIGR00512 241 AANGdTANKIGTYQLAILAKHHGVPFYVAAPTSTIDLElADGAEIPIEERPPEEVTHVGGVRIAPPGIGVWNPAFDVTPA 320

                         330
                  ....*....|....
gi 973165470  317 SLIAAIITEDGLIR 330
Cdd:TIGR00512 321 ELITGIITEKGVIT 334
eIF-2B_rel TIGR00524
eIF-2B alpha/beta/delta-related uncharacterized proteins; This model, eIF-2B_rel, describes ...
 31-329 1.70e-134

eIF-2B alpha/beta/delta-related uncharacterized proteins; This model, eIF-2B_rel, describes half of a superfamily, where the other half consists of eukaryotic translation initiation factor 2B (eIF-2B) subunits alpha, beta, and delta. It is unclear whether the eIF-2B_rel set is monophyletic, or whether they are all more closely related to each other than to any eIF-2B subunit because the eIF-2B clade is highly derived. Members of this branch of the family are all uncharacterized with respect to function and are found in the Archaea, Bacteria, and Eukarya, although a number are described as putative translation intiation factor components. Proteins found by eIF-2B_rel include at least three clades, including a set of uncharacterized eukaryotic proteins, a set found in some but not all Archaea, and a set universal so far among the Archaea and closely related to several uncharacterized bacterial proteins. [Unknown function, General]


Pssm-ID: 273119 [Multi-domain]  Cd Length: 303  Bit Score: 384.87  E-value: 1.70e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   31 CSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAASTLRSARPTAVNLAWGVDRVLSAIQPV 110
Cdd:TIGR00524   1 CRTYEDVAEAIKSMVVRGAPAIGVAAAYGLALAALKISHVNVEEFKEDLEKAADFLLSTRPTAVNLFWALDRVLNSLKSG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  111 SDPVDAAERALTMAESIADEDEATCRRIGSHGAPLLPDDCTVLTHCNAGALACAAWGTALGVIRSAAEEGKNIRVISCET 190
Cdd:TIGR00524  81 ESVEEFKESLLREAIEIINEDLETNRKIGENGAKLIKDGDTVLTHCNAGALATSGYGTALGVIRSAWEDGKRIRVIADET 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  191 RPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADRICTDG-VFNKIGTYMHAVSASYHQIPFYVAAPR 269
Cdd:TIGR00524 161 RPRNQGSRLTAWELVQDGIPVTLITDSAAAYFMQTGEIDAVIVGADRIARNGdVANKIGTYQLAVLAKEFRIPFFVAAPL 240

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 973165470  270 STFDPEHRM-ADIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPFSLIAAIITEDGLI 329
Cdd:TIGR00524 241 STFDPKTSTgEDIIIEERDPEEVAQVGGVRIAPLGVKVYNPAFDITPHDLIDAIITEKGII 301
PRK08334 PRK08334
S-methyl-5-thioribose-1-phosphate isomerase;
5-333 3.53e-93

S-methyl-5-thioribose-1-phosphate isomerase;


Pssm-ID: 169386  Cd Length: 356  Bit Score: 281.85  E-value: 3.53e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   5 RTIWWEEDGVGLIEQTLLPEEYREVVCSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAAS 84
Cdd:PRK08334  15 RSVEYEEGKVYMIDQRLLPREFKVIELRTVEEVAEAIKTMTVRGAPAIGAAAAFGLALYAETSKAKTKDEFMDGFYKAYE 94

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  85 TLRSARPTAVNLAWGVDRVLSAIQP-VSDPVDAAERALTM-AESIADEDEATCRRIGSHGAPLLPDDcTVLTHCNAGALA 162
Cdd:PRK08334  95 TLKNTRPTAVNLFWALNRIKKLVEEhLEDPLDEIKRLIVEeAQKIADEDVEANLRMGHYGAEVLPEG-NVLTHCNAGSLA 173

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 163 CAAWGTALGVIRSAAEEGKNIRVISCETRPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADRICTDG 242
Cdd:PRK08334 174 TVHLGTVGAVLRVMHKDGTLKLLWVDETRPVLQGARLSAWEYHYDGIPLKLISDNMAGFVMQQGKVDAIIVGADRIVANG 253

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 243 VF-NKIGTYMHAVSASYHQIPFYVAAPRSTFDPEHRMA-DIIVEERGREEVASLFGRTTVPDaVPVVNYAFDGTPFSLIA 320
Cdd:PRK08334 254 DFaNKIGTYTLAVLAKEHGIPFFTVAPLSTIDMSLKSGkEIPIEERSPEEVLTCGGCRIAPD-VDVYNPAFDVTPHKYLT 332

                        330
                 ....*....|...
gi 973165470 321 AIITEDGLIREPF 333
Cdd:PRK08334 333 GIITDRGVVWPPF 345
IF-2B pfam01008
Initiation factor 2 subunit family; This family includes initiation factor 2B alpha, beta and ...
 45-329 3.88e-92

Initiation factor 2 subunit family; This family includes initiation factor 2B alpha, beta and delta subunits from eukaryotes, initiation factor 2B subunits 1 and 2 from archaebacteria and some proteins of unknown function from prokaryotes. Initiation factor 2 binds to Met-tRNA, GTP and the small ribosomal subunit. Members of this family have also been characterized as 5-methylthioribose- 1-phosphate isomerases, an enzyme of the methionine salvage pathway. The crystal structure of Ypr118w, a non-essential, low-copy number gene product from Saccharomyces cerevisiae, reveals a dimeric protein with two domains and a putative active site cleft.


Pssm-ID: 395798 [Multi-domain]  Cd Length: 281  Bit Score: 276.10  E-value: 3.88e-92
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   45 EVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAASTLRSARPTAVNLAWGVDRVL---SAIQPVSDPVDAAERAL 121
Cdd:pfam01008   1 KVRGSPAIAIAALEGLLLVIQDARTPTVAELKEQLRSAIEFLISARPTAVSLGNAIDRLLrivLALHSSSDVEEAKESLI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  122 TMAESIADEDEATCRRIGSHGAPLLPDDCTVLTHCNAgalacaawGTALGVIRSAAEEGKNIRVISCETRPLLQGsRLTA 201
Cdd:pfam01008  81 EAADEFIDEIEEARRKIGAIAAELIKDGDTILTHCNS--------GTVLGVLRAAHKEGKRFRVIVTESRPRLQG-RLTA 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  202 WELSRDGIPVTTIIDSEAAYLMQRgeIDCVIVGADRICTDG-VFNKIGTYMHAVSASYHQIPFYVAAPRSTFDPEHRM-A 279
Cdd:pfam01008 152 KELVQAGIPVTLITDSAVGYVMQE--VDKVIVGADRILANGgIANKIGTYQLALLAKAHNVPFYVVAETYKFDPRFPLdE 229

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|
gi 973165470  280 DIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPFSLIAAIITEDGLI 329
Cdd:pfam01008 230 DIFIEERDPEEVLYRTGVRIAPPNLKVRNPAFDYTPPELITLIITEVGVL 279
PRK06371 PRK06371
S-methyl-5-thioribose-1-phosphate isomerase;
5-330 1.66e-89

S-methyl-5-thioribose-1-phosphate isomerase;


Pssm-ID: 180547  Cd Length: 329  Bit Score: 271.39  E-value: 1.66e-89
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   5 RTIWWEEDGVGLIEQTLLPEEYREVVCSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNalegsesafSASVNEAAS 84
Cdd:PRK06371  13 KAVWYEDGEVKLIDQRKLPDKIEIFEAKNSDDVAYAIKNMVVRGAPAIGVTAAYGLAMASKN---------GENMDEAVE 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  85 TLRSARPTAVNLAWGVDrvlsAIQPVSDPVDAAERaltmaesIADEDEATCRRIGSHGAPLLPDDCTVLTHCNAGALACA 164
Cdd:PRK06371  84 KIRSTRPTAYDLFKAIR----YMNSNEFDMNAARR-------YAMEIIGRSKKIGEYGNELIKNGARILTHCNAGALAVV 152

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 165 AWGTALGVIRSAAEEGKNIRVISCETRPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADRICTDGVF 244
Cdd:PRK06371 153 DWGTALAPIRIAHRNGKNIFVFVDETRPRLQGARLTAWELAQEGIDHAIIADNAAGYFMRKKEIDLVIVGADRIASNGDF 232

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 245 -NKIGTYMHAVSASYHQIPFYVAAPRSTFDPEHRMAD-IIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPFSLIAAI 322
Cdd:PRK06371 233 aNKIGTYEKAVLAKVNGIPFYVAAPGSTFDFSIKSGDeIPIEERDENEVLEINGCRIGPQESHARNPAFDVTPNEYVTGF 312


                 ....*...
gi 973165470 323 ITEDGLIR 330
Cdd:PRK06371 313 ITEYGIFK 320
PRK05772 PRK05772
S-methyl-5-thioribose-1-phosphate isomerase;
5-334 6.16e-85

S-methyl-5-thioribose-1-phosphate isomerase;


Pssm-ID: 168237  Cd Length: 363  Bit Score: 260.85  E-value: 6.16e-85
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470   5 RTIWWEEDGVGLIEQTLLPEEYREVVCSDVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAAS 84
Cdd:PRK05772  17 LPIIWKDNTLTLLDQSLLPFETVYVDLKTVEEVALAIRNMQVRGAPAIGITAGYGMVLALIENNVKTLDDAIRELTRAKT 96

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  85 TLRSARPTAVNLAWGVDRVL-----SAIQPVSDPVDAAERALTM-AESIADEDEATCRRIGSHGAPLLPDDCTVLTHCNA 158
Cdd:PRK05772  97 ILDSARPTAVNLVWATSRMLnkaknTVESGNAKSVNELIELLKVeAKKIFEEEYDAEIQMGLYGLEKLNDGDTVLTQCNA 176

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 159 GALACA-AWGTALGVIRSAAEEGKNIRVISCETRPLLQGSRLTAWELSRDGIPVTTIIDSEAAYLMQRGEIDCVIVGADR 237
Cdd:PRK05772 177 GGLATGtGLGTALAPVKLAKALGMSVSVIAPETRPWLQGSRLTVYELMEEGIKVTLITDTAVGLVMYKDMVNNVMVGADR 256

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 238 ICTDG-VFNKIGTYMHAVSASYHQIPFYVAAPRSTFDPEHRMADIIVEERGREEVASLFGRTTVPDAVPVVNYAFDGTPF 316
Cdd:PRK05772 257 ILRDGhVFNKIGTFKEAVIAHELGIPFYALAPTSTFDLKSDVNDVKIEERDPNEVRTIRGVPITPEDVNVYNPVFDVTPP 336

                        330
                 ....*....|....*...
gi 973165470 317 SLIAAIITEDGLIREPFH 334
Cdd:PRK05772 337 KYITGIITEKGIIYPPFH 354
GCD2 COG1184
Translation initiation factor 2B subunit, eIF-2B alpha/beta/delta family [Translation, ...
 33-329 1.15e-66

Translation initiation factor 2B subunit, eIF-2B alpha/beta/delta family [Translation, ribosomal structure and biogenesis]; Translation initiation factor 2B subunit, eIF-2B alpha/beta/delta family is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 440797 [Multi-domain]  Cd Length: 304  Bit Score: 212.02  E-value: 1.15e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  33 DVPRLVQAIQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAASTLRSARPTAVNLAWGVDRVLSAIQPVSD 112
Cdd:COG1184    3 RVAETAEEIREMEIHGAARIARAAAEALRDLAERSRAADPEEFRRELEAAARALRRARPSAASLPNAVRRVLARVADGET 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 113 PVDAAERALTMAESIADEDEATCRRIGSHGAPLLPDDCTVLTHCNAgalacaawGTALGVIRSAAEEGKNIRVISCETRP 192
Cdd:COG1184   83 VEEAREAVLEAADEFIERAEEAKERAAEIAAKRIRDGDTILTHSNS--------STVLAAIEAAVPQGKDIRVYVTESRP 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 193 LLQGsRLTAWELSRDGIPVTTIIDSEAAYLMqrGEIDCVIVGADRICTDG-VFNKIGTYMHAVSASYHQIPFYVAAPRST 271
Cdd:COG1184  155 RYQG-RITARELAEAGVPVTLIVDSAARHFL--KEVDRVVVGADTITADGaVVNKIGTSPLALAAREAGVPVTVAAETYK 231

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 973165470 272 FDPE-HRMADIivEERGREEVAslfgRTTVPDaVPVVNYAFDGTPFSLIAAIITEDGLI 329
Cdd:COG1184  232 FSPEtFELVEI--EERDPSEVY----DEEPEG-VTVRNPAFDVTPPDLIDAIITERGVL 283
PRK08535 PRK08535
ribose 1,5-bisphosphate isomerase;
41-329 7.49e-60

ribose 1,5-bisphosphate isomerase;


Pssm-ID: 236282 [Multi-domain]  Cd Length: 310  Bit Score: 194.35  E-value: 7.49e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  41 IQRLEVRGAPALGVAGAYGVALACKNALEGSESAFSASVNEAASTLRSARPTAVNLAWGVDRVLSAIQpvSDPVDAAERA 120
Cdd:PRK08535  14 IKTMEIRGAGRIARAAAEALKDQAEKSDAESPEEFKAEMRAAANILISTRPTAVSLPNAVRYVMRYYS--GETVEEARES 91

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 121 LTMA--ESIADEDEATcRRIGSHGAPLLPDDCTVLTHCNAGAlacaawgtALGVIRSAAEEGKNIRVISCETRPLLQGsR 198
Cdd:PRK08535  92 VIERaeEFIESSENAV-EKIGEIGAKRIRDGDVIMTHCNSSA--------ALSVIKTAHEQGKDIEVIATETRPRNQG-H 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 199 LTAWELSRDGIPVTTIIDSEAAYLMQrgEIDCVIVGADRICTDG-VFNKIGTYMHAVSASYHQIPFYVAAPRSTFDPEHR 277
Cdd:PRK08535 162 ITAKELAEYGIPVTLIVDSAVRYFMK--DVDKVVVGADAITANGaVINKIGTSQIALAAHEARVPFMVAAETYKFSPKTL 239

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|...
gi 973165470 278 MADII-VEERGREEVASLFGRTTVPDaVPVVNYAFDGTPFSLIAAIITEDGLI 329
Cdd:PRK08535 240 LGELVeIEERDPTEVLPEEILAKLPG-VKVRNPAFDVTPPEYIDAIITEIGAI 291
PRK08335 PRK08335
translation initiation factor IF-2B subunit alpha; Validated
 21-332 6.87e-30

translation initiation factor IF-2B subunit alpha; Validated


Pssm-ID: 169387  Cd Length: 275  Bit Score: 115.26  E-value: 6.87e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470  21 LLPEEYREvvcsdvprLVQAIQRLEVRGAPALGVAGAYgVALACKNALEGSEsafsasVNEAASTLRSARPtAVNLAWGV 100
Cdd:PRK08335   1 MLPPEVRE--------ILEEMKAERIRGASWLAKKGAE-AYLLLAEELDGEE------LENALKELREEIP-EVNPTMAS 64

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 101 DRVLSAIQPVSD-PVDAAERALtmaESIADEDEATcRRIGSHGAPLLPDDCTVLTHCNAGALacaawgtaLGVIRSAAEE 179
Cdd:PRK08335  65 LYNLARFIPITNnPELVKSRAE---EFLRLMEEAK-REIGNIGSELIDDGDVIITHSFSSAV--------LEILKTAKRK 132

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 180 GKNIRVISCETRPLLQGSRLtAWELSRDGIPVTTIIDSEAAYLMQRGEIdcVIVGADRICTDG-VFNKIGTYMHAVSASY 258
Cdd:PRK08335 133 GKRFKVILTESAPDYEGLAL-ANELEFLGIEFEVITDAQLGLFAKEATL--ALVGADNVTRDGyVVNKAGTYLLALACHD 209

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 973165470 259 HQIPFYVAAPRSTFDPEHRMADIIVEERGreevaslFGRttvpDAVPVVNYAFDGTPFSLIAAIITEDGLIREP 332
Cdd:PRK08335 210 NGVPFYVAAETFKFHPELKSEEVELVERP-------YAR----QGHRVRNVLFDVTPWKYVRGIITELGILVPP 272
PRK06372 PRK06372
translation initiation factor IF-2B subunit delta; Provisional
 175-330 7.55e-12

translation initiation factor IF-2B subunit delta; Provisional


Pssm-ID: 235788 [Multi-domain]  Cd Length: 253  Bit Score: 64.54  E-value: 7.55e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 175 SAAEEGKNIRVIscETRPLLQGSRLtAWELSRDGIPVTTIIDSEAAYLMQRgeIDCVIVGADRICTD-GVFNKIGTYMHA 253
Cdd:PRK06372 104 SSSEKIKSVYIL--ESRPMLEGIDM-AKLLVKSGIDVVLLTDASMCEAVLN--VDAVIVGSDSVLYDgGLIHKNGTFPLA 178

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 973165470 254 VSASYHQIPFYVAAPRSTFdpehrmadiiveERGREEVA----SLFGRTTVPDAVPVVNYAFDGTPFSLIAAIITEDGLI 329
Cdd:PRK06372 179 LCARYLKKPFYSLTISMKI------------ERNFLYSTypnfKNHPCSEWNIDIPCINRYFDKTPPDLIDYYINENGFV 246


                 .
gi 973165470 330 R 330
Cdd:PRK06372 247 K 247
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH