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Conserved domains on  [gi|972373254|ref|WP_059027154|]
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phage holin family protein [Clostridioides difficile]

Protein Classification

phage holin family protein( domain architecture ID 11240848)

phage holin family protein may function in the transport of murein hydrolases (endolysins) across the cytoplasmic membrane to the cell wall where these enzymes hydrolyze the cell wall polymer as a prelude to cell lysis

Gene Ontology:  GO:0016020|GO:0005886
PubMed:  7669346

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Phage_holin_5_2 pfam16079
Phage holin family Hol44, in holin superfamily V; Phage_holin_V_2 is a family of small ...
 15-80 5.50e-20

Phage holin family Hol44, in holin superfamily V; Phage_holin_V_2 is a family of small hydrophobic proteins with three transmembrane domains of the Hol44 family. These proteins are produced by double-stranded DNA bacteriophages that use an endolysin-holin strategy to achieve lysis of their hosts. The endolysins are peptidoglycan-degrading enzymes that are usually accumulated in the cytosol until access to the cell wall substrate is provided by the holin membrane lesion. Full activity of the endolysin Lys44 from oenophage fOg44 requires sudden ion-nonspecific dissipation of the proton motive force, undertaken by the fOg44 holin during phage-infection.


:

Pssm-ID: 465009  Cd Length: 66  Bit Score: 75.31  E-value: 5.50e-20
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 972373254  15 LVAALYVLGVGFKKYKQLDNKYIPVVLLILGIGFSIWMLGLNPVAILQGVICWGVAIGINQTYKQL 80
Cdd:pfam16079  1 LVPVLYLIGYIIKKTPKIKDKYIPLILGVVGILLGIAIFGFNVEAIIQGILSGGASTGGNQLFKQL 66
 
Name Accession Description Interval E-value
Phage_holin_5_2 pfam16079
Phage holin family Hol44, in holin superfamily V; Phage_holin_V_2 is a family of small ...
 15-80 5.50e-20

Phage holin family Hol44, in holin superfamily V; Phage_holin_V_2 is a family of small hydrophobic proteins with three transmembrane domains of the Hol44 family. These proteins are produced by double-stranded DNA bacteriophages that use an endolysin-holin strategy to achieve lysis of their hosts. The endolysins are peptidoglycan-degrading enzymes that are usually accumulated in the cytosol until access to the cell wall substrate is provided by the holin membrane lesion. Full activity of the endolysin Lys44 from oenophage fOg44 requires sudden ion-nonspecific dissipation of the proton motive force, undertaken by the fOg44 holin during phage-infection.


Pssm-ID: 465009  Cd Length: 66  Bit Score: 75.31  E-value: 5.50e-20
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 972373254  15 LVAALYVLGVGFKKYKQLDNKYIPVVLLILGIGFSIWMLGLNPVAILQGVICWGVAIGINQTYKQL 80
Cdd:pfam16079  1 LVPVLYLIGYIIKKTPKIKDKYIPLILGVVGILLGIAIFGFNVEAIIQGILSGGASTGGNQLFKQL 66
 
Name Accession Description Interval E-value
Phage_holin_5_2 pfam16079
Phage holin family Hol44, in holin superfamily V; Phage_holin_V_2 is a family of small ...
 15-80 5.50e-20

Phage holin family Hol44, in holin superfamily V; Phage_holin_V_2 is a family of small hydrophobic proteins with three transmembrane domains of the Hol44 family. These proteins are produced by double-stranded DNA bacteriophages that use an endolysin-holin strategy to achieve lysis of their hosts. The endolysins are peptidoglycan-degrading enzymes that are usually accumulated in the cytosol until access to the cell wall substrate is provided by the holin membrane lesion. Full activity of the endolysin Lys44 from oenophage fOg44 requires sudden ion-nonspecific dissipation of the proton motive force, undertaken by the fOg44 holin during phage-infection.


Pssm-ID: 465009  Cd Length: 66  Bit Score: 75.31  E-value: 5.50e-20
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 972373254  15 LVAALYVLGVGFKKYKQLDNKYIPVVLLILGIGFSIWMLGLNPVAILQGVICWGVAIGINQTYKQL 80
Cdd:pfam16079  1 LVPVLYLIGYIIKKTPKIKDKYIPLILGVVGILLGIAIFGFNVEAIIQGILSGGASTGGNQLFKQL 66
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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