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Conserved domains on  [gi|910231435|ref|WP_050036610|]
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MULTISPECIES: N-6 DNA methylase [unclassified Haloarcula]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
HsdM super family cl33828
Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];
89-192 2.79e-17

Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];


The actual alignment was detected with superfamily member COG0286:

Pssm-ID: 440055 [Multi-domain]  Cd Length: 243  Bit Score: 78.31  E-value: 2.79e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 910231435  89 DVLGAVYEEY--GMSSDA---FGQHFTPHSVCETMAEIAgvggesDTDDRQTVLDPACGSGRTLLVA-------GRKQPD 156
Cdd:COG0286    1 DVLGDAYEYLlrKFAEESgkkAGEFYTPREVVRLMVELL------DPKPGETVYDPACGSGGFLVEAaeylkehGGDERK 74

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 910231435 157 AL-FVGQDKDPLCARMTALNCCFLNL-DAYVIQGDSLT 192
Cdd:COG0286   75 KLsLYGQEINPTTYRLAKMNLLLHGIgDPNIELGDTLS 112
 
Name Accession Description Interval E-value
HsdM COG0286
Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];
89-192 2.79e-17

Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];


Pssm-ID: 440055 [Multi-domain]  Cd Length: 243  Bit Score: 78.31  E-value: 2.79e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 910231435  89 DVLGAVYEEY--GMSSDA---FGQHFTPHSVCETMAEIAgvggesDTDDRQTVLDPACGSGRTLLVA-------GRKQPD 156
Cdd:COG0286    1 DVLGDAYEYLlrKFAEESgkkAGEFYTPREVVRLMVELL------DPKPGETVYDPACGSGGFLVEAaeylkehGGDERK 74

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 910231435 157 AL-FVGQDKDPLCARMTALNCCFLNL-DAYVIQGDSLT 192
Cdd:COG0286   75 KLsLYGQEINPTTYRLAKMNLLLHGIgDPNIELGDTLS 112
N6_Mtase pfam02384
N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against ...
 87-192 2.28e-12

N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The R-M system is a complex containing three polypeptides: M (this family), S (pfam01420), and R. This family consists of N-6 adenine-specific DNA methylase EC:2.1.1.72 from Type I and Type IC restriction systems. These methylases have the same sequence specificity as their corresponding restriction enzymes.


Pssm-ID: 426749 [Multi-domain]  Cd Length: 310  Bit Score: 65.42  E-value: 2.28e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 910231435   87 DADVLGAVYEeYGMSSDA------FGQHFTPHSVCETMAEIAgvggesDTDDRQTVLDPACGSGRTLLVAGR-------K 153
Cdd:pfam02384   1 SRDLFGDAYE-YLLRKFApnagksGGEFFTPREVSKLIVELL------DPKPGESIYDPACGSGGFLIQAEKfvkehdgD 73

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 910231435  154 QPDALFVGQDKDPLCARMTALNCCFLNLDAYVIQ---GDSLT 192
Cdd:pfam02384  74 TNDLSIYGQEKNPTTYRLARMNMILHGIEYDDFHirhGDTLT 115
hsdM TIGR00497
type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine ...
 89-192 1.49e-07

type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine residues; required for both restriction and modification activities. The ECOR124/3 I enzyme recognizes 5'GAA(N7)RTCG. for E.coli see (J. Mol. Biol. 257: 960-969 (1996)). [DNA metabolism, Restriction/modification]


Pssm-ID: 211578 [Multi-domain]  Cd Length: 501  Bit Score: 51.88  E-value: 1.49e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 910231435   89 DVLGAVYEeYGMSSDAF------GQHFTPHSVCETMAEIAgVGGESDTDDrqtVLDPACGSGRTLL----VAGRKQPDAL 158
Cdd:TIGR00497 173 DAFGDAYE-FLISMYAQnagksgGEFFTPQDISELLARIA-IGKKDTVDD---VYDMACGSGSLLLqvikVLGEKTSLVS 247

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 910231435  159 FVGQDKDplcarMTALNCCFLNL--------DAYVIQGDSLT 192
Cdd:TIGR00497 248 YYGQEIN-----HTTYNLCRMNMilhnidyaNFNIINADTLT 284
 
Name Accession Description Interval E-value
HsdM COG0286
Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];
89-192 2.79e-17

Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];


Pssm-ID: 440055 [Multi-domain]  Cd Length: 243  Bit Score: 78.31  E-value: 2.79e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 910231435  89 DVLGAVYEEY--GMSSDA---FGQHFTPHSVCETMAEIAgvggesDTDDRQTVLDPACGSGRTLLVA-------GRKQPD 156
Cdd:COG0286    1 DVLGDAYEYLlrKFAEESgkkAGEFYTPREVVRLMVELL------DPKPGETVYDPACGSGGFLVEAaeylkehGGDERK 74

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 910231435 157 AL-FVGQDKDPLCARMTALNCCFLNL-DAYVIQGDSLT 192
Cdd:COG0286   75 KLsLYGQEINPTTYRLAKMNLLLHGIgDPNIELGDTLS 112
N6_Mtase pfam02384
N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against ...
 87-192 2.28e-12

N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The R-M system is a complex containing three polypeptides: M (this family), S (pfam01420), and R. This family consists of N-6 adenine-specific DNA methylase EC:2.1.1.72 from Type I and Type IC restriction systems. These methylases have the same sequence specificity as their corresponding restriction enzymes.


Pssm-ID: 426749 [Multi-domain]  Cd Length: 310  Bit Score: 65.42  E-value: 2.28e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 910231435   87 DADVLGAVYEeYGMSSDA------FGQHFTPHSVCETMAEIAgvggesDTDDRQTVLDPACGSGRTLLVAGR-------K 153
Cdd:pfam02384   1 SRDLFGDAYE-YLLRKFApnagksGGEFFTPREVSKLIVELL------DPKPGESIYDPACGSGGFLIQAEKfvkehdgD 73

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 910231435  154 QPDALFVGQDKDPLCARMTALNCCFLNLDAYVIQ---GDSLT 192
Cdd:pfam02384  74 TNDLSIYGQEKNPTTYRLARMNMILHGIEYDDFHirhGDTLT 115
hsdM TIGR00497
type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine ...
 89-192 1.49e-07

type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine residues; required for both restriction and modification activities. The ECOR124/3 I enzyme recognizes 5'GAA(N7)RTCG. for E.coli see (J. Mol. Biol. 257: 960-969 (1996)). [DNA metabolism, Restriction/modification]


Pssm-ID: 211578 [Multi-domain]  Cd Length: 501  Bit Score: 51.88  E-value: 1.49e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 910231435   89 DVLGAVYEeYGMSSDAF------GQHFTPHSVCETMAEIAgVGGESDTDDrqtVLDPACGSGRTLL----VAGRKQPDAL 158
Cdd:TIGR00497 173 DAFGDAYE-FLISMYAQnagksgGEFFTPQDISELLARIA-IGKKDTVDD---VYDMACGSGSLLLqvikVLGEKTSLVS 247

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 910231435  159 FVGQDKDplcarMTALNCCFLNL--------DAYVIQGDSLT 192
Cdd:TIGR00497 248 YYGQEIN-----HTTYNLCRMNMilhnidyaNFNIINADTLT 284
YtxK COG0827
Adenine-specific DNA N6-methylase [Replication, recombination and repair];
84-192 6.24e-06

Adenine-specific DNA N6-methylase [Replication, recombination and repair];


Pssm-ID: 440589 [Multi-domain]  Cd Length: 327  Bit Score: 46.48  E-value: 6.24e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 910231435  84 AATDADVLGAVYEEY--GMSSDAFGQH-FTPHSVCETMAEIAG-VGGESDtddrQTVLDPACGSGR---TLLVAGRKQPD 156
Cdd:COG0827   67 SLSKEEIRKALQLALlkGMKESVQPNHqMTPDAIGLLIGYLVEkFTKKEG----LRILDPAVGTGNlltTVLNQLKKKVN 142

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 910231435 157 AlfVGQDKDPLCARMTALNCCFLNLDAYVIQGDSLT 192
Cdd:COG0827  143 A--YGVEVDDLLIRLAAVLANLQGHPVELFHQDALQ 176
Trm11 COG1041
tRNA G10 N-methylase Trm11 [Translation, ribosomal structure and biogenesis]; tRNA G10 ...
 108-195 3.23e-04

tRNA G10 N-methylase Trm11 [Translation, ribosomal structure and biogenesis]; tRNA G10 N-methylase Trm11 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440663 [Multi-domain]  Cd Length: 172  Bit Score: 40.32  E-value: 3.23e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 910231435 108 HFTPHS----VCETMAEIAGVGgESDTddrqtVLDPACGSGRTLLVAGRKQPDAlfVGQDKDPLCARMTALNCCFLNL-D 182
Cdd:COG1041    4 FFYPGSldprLARALVNLAGAK-EGDT-----VLDPFCGTGTILIEAGLLGRRV--IGSDIDPKMVEGARENLEHYGYeD 75

                         90
                 ....*....|...
gi 910231435 183 AYVIQGDSLTVEF 195
Cdd:COG1041   76 ADVIRGDARDLPL 88
N6_N4_Mtase pfam01555
DNA methylase; Members of this family are DNA methylases. The family contains both N-4 ...
 101-166 1.76e-03

DNA methylase; Members of this family are DNA methylases. The family contains both N-4 cytosine-specific DNA methylases and N-6 Adenine-specific DNA methylases.


Pssm-ID: 396230 [Multi-domain]  Cd Length: 221  Bit Score: 38.53  E-value: 1.76e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 910231435  101 SSDAFGQHFTPHSVcETMAEIAgvggESDTDDRQTVLDPACGSGRTLLVA---GRKqpdalFVGQDKDP 166
Cdd:pfam01555 155 ESGGNGKHPTQKPE-ALLERLI----LASTNPGDIVLDPFAGSGTTGAAAkelGRN-----FIGIEIEE 213
YhdJ COG0863
DNA modification methylase [Replication, recombination and repair];
134-166 5.47e-03

DNA modification methylase [Replication, recombination and repair];


Pssm-ID: 440623  Cd Length: 236  Bit Score: 37.21  E-value: 5.47e-03
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 910231435 134 QTVLDPACGSGRTLLVA---GRKqpdalFVGQDKDP 166
Cdd:COG0863  184 DIVLDPFAGSGTTLVAAerlGRR-----FIGIEIDP 214
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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