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Conserved domains on  [gi|908744126|ref|WP_049866481|]
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succinylglutamate desuccinylase [Pseudoalteromonas sp. R96]

Protein Classification

succinylglutamate desuccinylase( domain architecture ID 10012321)

succinylglutamate desuccinylase catalyzes the formation of succinate and L-glutamate from N-succinyl-L-glutamate in the fifth and final reaction of the ammonia-producing arginine catabolic pathway, L-arginine degradation II (AST pathway)

EC:  3.5.1.96
Gene Ontology:  GO:0008270|GO:0016788|GO:0009017|GO:0019545

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK05324 PRK05324
succinylglutamate desuccinylase; Provisional
 6-339 1.07e-172

succinylglutamate desuccinylase; Provisional


:

Pssm-ID: 235408  Cd Length: 329  Bit Score: 482.76  E-value: 1.07e-172
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126   6 LKQTGDFLTLTRNNEFSlAAEQFTLSNGTLVEVKDTGVIQFTPATYGDKDIVLSSAVHGNETAPIEICDAFIQDVILQRI 85
Cdd:PRK05324   1 MLAMDDFLALTLAGHPP-AVTEFGLGNGVRWRWLGEGVLELTPAAPSTKALVLSAGIHGNETAPIELLDQLVRDLLAGEL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  86 ELAHRVLFIFGNPKSINIAERFVDENLNRLFNGAHENRTDNPEQIRAAKLESYVSDFFtSVPEGRYRCHYDLHTAIRGSK 165
Cdd:PRK05324  80 PLRARLLVILGNPPAMRAGKRYLDEDLNRLFGGRHQQFPGSDEARRAAELEQAVEDFF-AAGAERVRWHYDLHTAIRGSK 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 166 NEKFAVYPFlHGKPWKKSQLQFMLACGVNTILMMRSAATTFSYFSSFQFGADAFTVELGQVKPFGQNDMSRFSAVKQTLK 245
Cdd:PRK05324 159 HEQFAVLPQ-RGRPWSREQLAWLGAAGIEAVLLHNAPGGTFSHFSSEHFGALACTLELGKVLPFGQNDLSRFAATDQALR 237

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 246 ALISQEsvEYDEFNASDFELFQVHRTINRTCDAFSFPFPDSAVNFTGFAKGELLATDGDTTYFAEVEGEAIIFPNAKVAL 325
Cdd:PRK05324 238 ALISGE--ELPERSADPPRLYRVVRQITKHSDDFELHFADDVENFTAFPKGTLLAEDGDERYVVEHDGERIVFPNPNVAI 315

                        330
                 ....*....|....
gi 908744126 326 GQRALLTVVPLEVD 339
Cdd:PRK05324 316 GLRAGLMLVPTTLD 329
 
Name Accession Description Interval E-value
PRK05324 PRK05324
succinylglutamate desuccinylase; Provisional
 6-339 1.07e-172

succinylglutamate desuccinylase; Provisional


Pssm-ID: 235408  Cd Length: 329  Bit Score: 482.76  E-value: 1.07e-172
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126   6 LKQTGDFLTLTRNNEFSlAAEQFTLSNGTLVEVKDTGVIQFTPATYGDKDIVLSSAVHGNETAPIEICDAFIQDVILQRI 85
Cdd:PRK05324   1 MLAMDDFLALTLAGHPP-AVTEFGLGNGVRWRWLGEGVLELTPAAPSTKALVLSAGIHGNETAPIELLDQLVRDLLAGEL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  86 ELAHRVLFIFGNPKSINIAERFVDENLNRLFNGAHENRTDNPEQIRAAKLESYVSDFFtSVPEGRYRCHYDLHTAIRGSK 165
Cdd:PRK05324  80 PLRARLLVILGNPPAMRAGKRYLDEDLNRLFGGRHQQFPGSDEARRAAELEQAVEDFF-AAGAERVRWHYDLHTAIRGSK 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 166 NEKFAVYPFlHGKPWKKSQLQFMLACGVNTILMMRSAATTFSYFSSFQFGADAFTVELGQVKPFGQNDMSRFSAVKQTLK 245
Cdd:PRK05324 159 HEQFAVLPQ-RGRPWSREQLAWLGAAGIEAVLLHNAPGGTFSHFSSEHFGALACTLELGKVLPFGQNDLSRFAATDQALR 237

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 246 ALISQEsvEYDEFNASDFELFQVHRTINRTCDAFSFPFPDSAVNFTGFAKGELLATDGDTTYFAEVEGEAIIFPNAKVAL 325
Cdd:PRK05324 238 ALISGE--ELPERSADPPRLYRVVRQITKHSDDFELHFADDVENFTAFPKGTLLAEDGDERYVVEHDGERIVFPNPNVAI 315

                        330
                 ....*....|....
gi 908744126 326 GQRALLTVVPLEVD 339
Cdd:PRK05324 316 GLRAGLMLVPTTLD 329
AstE COG2988
Succinylglutamate desuccinylase [Amino acid transport and metabolism];
34-339 1.75e-134

Succinylglutamate desuccinylase [Amino acid transport and metabolism];


Pssm-ID: 442227  Cd Length: 305  Bit Score: 384.97  E-value: 1.75e-134
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  34 TLVEVKDTGVIQFTPATYGDKDIVLSSAVHGNETAPIEICDAFIQDVILQRIELAHRVLFIFGNPKSINIAERFVDENLN 113
Cdd:COG2988    5 ALTRWLDEGVLELTPHAPGIKAVVISGGIHGNETAPIELLDKLLQDLLLGERPLSFRLLLILGNPAAMRAGRRYLDEDLN 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 114 RLFNGAHENRTDNPEQIRAAKLESYVSDFFTSvpEGRYRCHYDLHTAIRGSKNEKFAVYPFlHGKPWKKSQLQFMLACGV 193
Cdd:COG2988   85 RLFGGRHLQNPESYEAARAKELEQAVGPFFAA--GGRVRLHIDLHTAIRNSGHERFAVYPF-RGRPFDLALLAYLAAAGP 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 194 NTILMMRSAATTFSYFSSFQFGADAFTVELGQVKPFGQNDMSRFSAVKQTLKALISQEsvEYDEFNASDFELFQVHRTIN 273
Cdd:COG2988  162 EAVVLHHAPGGTFSHFSAELCGAQAFTLELGKVRPFGQNDLSRFAATEEALRALLSGA--ELPEHPAQDLDLYRVVQQII 239

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 908744126 274 RTCDAFSFPFPDSAVNFTGFAKGELLATDGDTTYFAEVEGEAIIFPNAKVALGQRALLTVVPLEVD 339
Cdd:COG2988  240 KHGDDFMLHPDLDTLNFTPLPPGTLLAEDGGKEYRVEGDEERIVFPNEAVYYGQRAALLLTPKELI 305
M14_ASTE cd03855
Peptidase M14 Succinylglutamate desuccinylase (ASTE) subfamily; Peptidase M14 ...
 11-250 2.19e-124

Peptidase M14 Succinylglutamate desuccinylase (ASTE) subfamily; Peptidase M14 Succinylglutamate desuccinylase (ASTE, also known as N-succinyl-L-glutamate amidohydrolase, N2-succinylglutamate desuccinylase, and SGDS; EC 3.5.1.96) belongs to the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily of the M14 family of metallocarboxypeptidases. This group includes succinylglutamate desuccinylase that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. It hydrolyzes N-succinyl-L-glutamate to succinate and L-glutamate.


Pssm-ID: 349428  Cd Length: 239  Bit Score: 356.90  E-value: 2.19e-124
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  11 DFLTLTRNNEFSLAAEQFTLSNGTLVEVKDTGVIQFTPATYGDKDIVLSSAVHGNETAPIEICDAFIQDVILQRIELAHR 90
Cdd:cd03855    1 DFLALTLSGSEPAEGELAAVSNGTRVRWLATGVLELTPAASASKSVVLSAGIHGNETAPIEILDQLINDLIRGELALAHR 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  91 VLFIFGNPKSINIAERFVDENLNRLFNGAHENRTDNPEQIRAAKLESYVSDFFTSvPEGRYRCHYDLHTAIRGSKNEKFA 170
Cdd:cd03855   81 LLFIFGNPPAIRQGKRFIEENLNRLFSGRHSKLPPSYETARAAELEQAVADFFAK-ASGEVRWHLDLHTAIRGSKHEQFA 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 171 VYPFLHGKPWKKSQLQFMLACGVNTILMMRSAATTFSYFSSFQFGADAFTVELGQVKPFGQNDMSRFSAVKQTLKALISQ 250
Cdd:cd03855  160 VYPFLEGRPHDREQLDFLGAAGIEAVLLSNSPGGTFSYYSSEHFGAAAFTVELGKVRPFGQNDLSQFAAIDQALRALISG 239
arg_catab_astE TIGR03242
succinylglutamate desuccinylase; Members of this protein family are succinylglutamate ...
 11-334 1.50e-122

succinylglutamate desuccinylase; Members of this protein family are succinylglutamate desuccinylase, the fifth and final enzyme of the arginine succinyltransferase (AST) pathway for arginine catabolism. This model excludes the related protein aspartoacylase. [Energy metabolism, Amino acids and amines]


Pssm-ID: 132286  Cd Length: 319  Bit Score: 355.14  E-value: 1.50e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126   11 DFLTLTRNNEFSLAaeQFTLSNGTLVEVKDTGVIQFTPATYGDKDIVLSSAVHGNETAPIEICDAFIQDVILQRIELAHR 90
Cdd:TIGR03242   1 DFLALTLTGKKPHV--TQGETNNVRWRWLGEGVLELTPHAPPQKSLVISAGIHGNETAPIEILEQLLGDIAAGKLPLRVR 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126   91 VLFIFGNPKSINIAERFVDENLNRLFNGAHENRTDNPEQIRAAKLESYVSDFFTSVPEGRYRCHYDLHTAIRGSKNEKFA 170
Cdd:TIGR03242  79 LLVILGNPPAMRTGKRYLHDDLNRMFGGRYQQLAPSFETCRAAELEQCVEDFFSQGGRSVARWHYDLHTAIRGSLHEQFA 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  171 VYPFLhGKPWKKSQLQFMLACGVNTILMMRSAATTFSYFSSFQFGADAFTVELGQVKPFGQNDMSRFSAVKQTLKALISQ 250
Cdd:TIGR03242 159 LLPYQ-GRPWDREFLTWLGAAGLDALVFHTEPGGTFSHFSSEHFGALACTLELGKALPFGQNDLSQFAAITSALRALISD 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  251 ESVEYDEFNasDFELFQVHRTINRTCDAFSFPFPDSAVNFTGFAKGELLATDGDTTYFAEVEGEAIIFPNAKVALGQRAL 330
Cdd:TIGR03242 238 EAIPARRTD--PLRLFRVVSSITKHSDSFELHVASDTLNFTPFPKGTLLATDGNERYRVTHEGERILFPNPNVANGLRAG 315


                  ....
gi 908744126  331 LTVV 334
Cdd:TIGR03242 316 LMLV 319
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 54-335 5.00e-61

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 197.19  E-value: 5.00e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126   54 KDIVLSSAVHGNETAPIEICDAFIQDVILQRIeLAHRVLFIFGNPKSINIAERFVDENLNRLFNGAHENRTdNPEQIRAA 133
Cdd:pfam04952   3 PTLLLSAGIHGNETNGVELLRRLLRQLDPGDI-AGERTLVPLANPPAFRAGSRYIPRDLNRSFPGRALGAS-SDEPYRAT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  134 KLESYVSDFFTSVPEgRYRCHYDLHTAIRGSKNEKFAVYPFlhgKPWKKSQLQFMLACGVNTILMMRSA-ATTFSYFSSF 212
Cdd:pfam04952  81 RAERLADLFFPALLP-RADIVLDLHTGTRGMGHLLFALAPI---RDDPLHLLALLRAFGAPAVLKLHSKpSAGFSAFSAE 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  213 QFGADAFTVELGQVKPFGQNDMSRFSAVKQTLKALISQESVEYDEFNASdfELFQVHRTINRTCDAFS---------FPF 283
Cdd:pfam04952 157 ELGAPGFTLELGGAGPFGANLISRTAAGVLNVLRLIGVLNGGPDAFEPP--KLYRVLREIDRPRDIRAelaglvefaLNL 234

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|...
gi 908744126  284 PDSAVNFTGFAKGELLATD-GDTTYFAEVEGEAIIFPNAKVALGQRALLTVVP 335
Cdd:pfam04952 235 GDDVDAGPLLPGGPLFAPFgGEETEYRAPEDGYPVFPNEAAYVGKGAALALVA 287
 
Name Accession Description Interval E-value
PRK05324 PRK05324
succinylglutamate desuccinylase; Provisional
 6-339 1.07e-172

succinylglutamate desuccinylase; Provisional


Pssm-ID: 235408  Cd Length: 329  Bit Score: 482.76  E-value: 1.07e-172
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126   6 LKQTGDFLTLTRNNEFSlAAEQFTLSNGTLVEVKDTGVIQFTPATYGDKDIVLSSAVHGNETAPIEICDAFIQDVILQRI 85
Cdd:PRK05324   1 MLAMDDFLALTLAGHPP-AVTEFGLGNGVRWRWLGEGVLELTPAAPSTKALVLSAGIHGNETAPIELLDQLVRDLLAGEL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  86 ELAHRVLFIFGNPKSINIAERFVDENLNRLFNGAHENRTDNPEQIRAAKLESYVSDFFtSVPEGRYRCHYDLHTAIRGSK 165
Cdd:PRK05324  80 PLRARLLVILGNPPAMRAGKRYLDEDLNRLFGGRHQQFPGSDEARRAAELEQAVEDFF-AAGAERVRWHYDLHTAIRGSK 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 166 NEKFAVYPFlHGKPWKKSQLQFMLACGVNTILMMRSAATTFSYFSSFQFGADAFTVELGQVKPFGQNDMSRFSAVKQTLK 245
Cdd:PRK05324 159 HEQFAVLPQ-RGRPWSREQLAWLGAAGIEAVLLHNAPGGTFSHFSSEHFGALACTLELGKVLPFGQNDLSRFAATDQALR 237

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 246 ALISQEsvEYDEFNASDFELFQVHRTINRTCDAFSFPFPDSAVNFTGFAKGELLATDGDTTYFAEVEGEAIIFPNAKVAL 325
Cdd:PRK05324 238 ALISGE--ELPERSADPPRLYRVVRQITKHSDDFELHFADDVENFTAFPKGTLLAEDGDERYVVEHDGERIVFPNPNVAI 315

                        330
                 ....*....|....
gi 908744126 326 GQRALLTVVPLEVD 339
Cdd:PRK05324 316 GLRAGLMLVPTTLD 329
AstE COG2988
Succinylglutamate desuccinylase [Amino acid transport and metabolism];
34-339 1.75e-134

Succinylglutamate desuccinylase [Amino acid transport and metabolism];


Pssm-ID: 442227  Cd Length: 305  Bit Score: 384.97  E-value: 1.75e-134
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  34 TLVEVKDTGVIQFTPATYGDKDIVLSSAVHGNETAPIEICDAFIQDVILQRIELAHRVLFIFGNPKSINIAERFVDENLN 113
Cdd:COG2988    5 ALTRWLDEGVLELTPHAPGIKAVVISGGIHGNETAPIELLDKLLQDLLLGERPLSFRLLLILGNPAAMRAGRRYLDEDLN 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 114 RLFNGAHENRTDNPEQIRAAKLESYVSDFFTSvpEGRYRCHYDLHTAIRGSKNEKFAVYPFlHGKPWKKSQLQFMLACGV 193
Cdd:COG2988   85 RLFGGRHLQNPESYEAARAKELEQAVGPFFAA--GGRVRLHIDLHTAIRNSGHERFAVYPF-RGRPFDLALLAYLAAAGP 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 194 NTILMMRSAATTFSYFSSFQFGADAFTVELGQVKPFGQNDMSRFSAVKQTLKALISQEsvEYDEFNASDFELFQVHRTIN 273
Cdd:COG2988  162 EAVVLHHAPGGTFSHFSAELCGAQAFTLELGKVRPFGQNDLSRFAATEEALRALLSGA--ELPEHPAQDLDLYRVVQQII 239

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 908744126 274 RTCDAFSFPFPDSAVNFTGFAKGELLATDGDTTYFAEVEGEAIIFPNAKVALGQRALLTVVPLEVD 339
Cdd:COG2988  240 KHGDDFMLHPDLDTLNFTPLPPGTLLAEDGGKEYRVEGDEERIVFPNEAVYYGQRAALLLTPKELI 305
M14_ASTE cd03855
Peptidase M14 Succinylglutamate desuccinylase (ASTE) subfamily; Peptidase M14 ...
 11-250 2.19e-124

Peptidase M14 Succinylglutamate desuccinylase (ASTE) subfamily; Peptidase M14 Succinylglutamate desuccinylase (ASTE, also known as N-succinyl-L-glutamate amidohydrolase, N2-succinylglutamate desuccinylase, and SGDS; EC 3.5.1.96) belongs to the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily of the M14 family of metallocarboxypeptidases. This group includes succinylglutamate desuccinylase that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. It hydrolyzes N-succinyl-L-glutamate to succinate and L-glutamate.


Pssm-ID: 349428  Cd Length: 239  Bit Score: 356.90  E-value: 2.19e-124
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  11 DFLTLTRNNEFSLAAEQFTLSNGTLVEVKDTGVIQFTPATYGDKDIVLSSAVHGNETAPIEICDAFIQDVILQRIELAHR 90
Cdd:cd03855    1 DFLALTLSGSEPAEGELAAVSNGTRVRWLATGVLELTPAASASKSVVLSAGIHGNETAPIEILDQLINDLIRGELALAHR 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  91 VLFIFGNPKSINIAERFVDENLNRLFNGAHENRTDNPEQIRAAKLESYVSDFFTSvPEGRYRCHYDLHTAIRGSKNEKFA 170
Cdd:cd03855   81 LLFIFGNPPAIRQGKRFIEENLNRLFSGRHSKLPPSYETARAAELEQAVADFFAK-ASGEVRWHLDLHTAIRGSKHEQFA 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 171 VYPFLHGKPWKKSQLQFMLACGVNTILMMRSAATTFSYFSSFQFGADAFTVELGQVKPFGQNDMSRFSAVKQTLKALISQ 250
Cdd:cd03855  160 VYPFLEGRPHDREQLDFLGAAGIEAVLLSNSPGGTFSYYSSEHFGAAAFTVELGKVRPFGQNDLSQFAAIDQALRALISG 239
arg_catab_astE TIGR03242
succinylglutamate desuccinylase; Members of this protein family are succinylglutamate ...
 11-334 1.50e-122

succinylglutamate desuccinylase; Members of this protein family are succinylglutamate desuccinylase, the fifth and final enzyme of the arginine succinyltransferase (AST) pathway for arginine catabolism. This model excludes the related protein aspartoacylase. [Energy metabolism, Amino acids and amines]


Pssm-ID: 132286  Cd Length: 319  Bit Score: 355.14  E-value: 1.50e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126   11 DFLTLTRNNEFSLAaeQFTLSNGTLVEVKDTGVIQFTPATYGDKDIVLSSAVHGNETAPIEICDAFIQDVILQRIELAHR 90
Cdd:TIGR03242   1 DFLALTLTGKKPHV--TQGETNNVRWRWLGEGVLELTPHAPPQKSLVISAGIHGNETAPIEILEQLLGDIAAGKLPLRVR 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126   91 VLFIFGNPKSINIAERFVDENLNRLFNGAHENRTDNPEQIRAAKLESYVSDFFTSVPEGRYRCHYDLHTAIRGSKNEKFA 170
Cdd:TIGR03242  79 LLVILGNPPAMRTGKRYLHDDLNRMFGGRYQQLAPSFETCRAAELEQCVEDFFSQGGRSVARWHYDLHTAIRGSLHEQFA 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  171 VYPFLhGKPWKKSQLQFMLACGVNTILMMRSAATTFSYFSSFQFGADAFTVELGQVKPFGQNDMSRFSAVKQTLKALISQ 250
Cdd:TIGR03242 159 LLPYQ-GRPWDREFLTWLGAAGLDALVFHTEPGGTFSHFSSEHFGALACTLELGKALPFGQNDLSQFAAITSALRALISD 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  251 ESVEYDEFNasDFELFQVHRTINRTCDAFSFPFPDSAVNFTGFAKGELLATDGDTTYFAEVEGEAIIFPNAKVALGQRAL 330
Cdd:TIGR03242 238 EAIPARRTD--PLRLFRVVSSITKHSDSFELHVASDTLNFTPFPKGTLLATDGNERYRVTHEGERILFPNPNVANGLRAG 315


                  ....
gi 908744126  331 LTVV 334
Cdd:TIGR03242 316 LMLV 319
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 54-335 5.00e-61

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 197.19  E-value: 5.00e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126   54 KDIVLSSAVHGNETAPIEICDAFIQDVILQRIeLAHRVLFIFGNPKSINIAERFVDENLNRLFNGAHENRTdNPEQIRAA 133
Cdd:pfam04952   3 PTLLLSAGIHGNETNGVELLRRLLRQLDPGDI-AGERTLVPLANPPAFRAGSRYIPRDLNRSFPGRALGAS-SDEPYRAT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  134 KLESYVSDFFTSVPEgRYRCHYDLHTAIRGSKNEKFAVYPFlhgKPWKKSQLQFMLACGVNTILMMRSA-ATTFSYFSSF 212
Cdd:pfam04952  81 RAERLADLFFPALLP-RADIVLDLHTGTRGMGHLLFALAPI---RDDPLHLLALLRAFGAPAVLKLHSKpSAGFSAFSAE 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  213 QFGADAFTVELGQVKPFGQNDMSRFSAVKQTLKALISQESVEYDEFNASdfELFQVHRTINRTCDAFS---------FPF 283
Cdd:pfam04952 157 ELGAPGFTLELGGAGPFGANLISRTAAGVLNVLRLIGVLNGGPDAFEPP--KLYRVLREIDRPRDIRAelaglvefaLNL 234

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|...
gi 908744126  284 PDSAVNFTGFAKGELLATD-GDTTYFAEVEGEAIIFPNAKVALGQRALLTVVP 335
Cdd:pfam04952 235 GDDVDAGPLLPGGPLFAPFgGEETEYRAPEDGYPVFPNEAAYVGKGAALALVA 287
COG3608 COG3608
Predicted deacylase [General function prediction only];
56-239 8.22e-14

Predicted deacylase [General function prediction only];


Pssm-ID: 442826 [Multi-domain]  Cd Length: 296  Bit Score: 70.65  E-value: 8.22e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  56 IVLSSAVHGNETAPIEICDAFIQDviLQRIELAHRVLFI-FGNPKSINIAERF---VDENLNRLFNGaheNRTDNPEQIR 131
Cdd:COG3608   29 LLITAGIHGDELNGIEALRRLLRE--LDPGELRGTVILVpVANPPGFLQGSRYlpiDGRDLNRSFPG---DADGSLAERI 103

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 132 AAKLESYVSDfftsvpegryRCHY--DLHTAIRGSKNEKFAVYPFLHGKpwkksQLQFMLACGVNTILMMR-SAATTFSY 208
Cdd:COG3608  104 AHALFEEILP----------DADYviDLHSGGIARDNLPHVRAGPGDEE-----LRALARAFGAPVILDSPeGGDGSLRE 168

                        170       180       190
                 ....*....|....*....|....*....|.
gi 908744126 209 fSSFQFGADAFTVELGqvkpfGQNDMSRFSA 239
Cdd:COG3608  169 -AAAEAGIPALTLELG-----GGGRFDEESI 193
M14_ASTE_ASPA_like cd06230
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The ...
 56-225 1.08e-10

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily belongs to the M14 family of metallocarboxypeptidases (MCPs), and includes ASTE, which catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) which cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349449 [Multi-domain]  Cd Length: 177  Bit Score: 59.63  E-value: 1.08e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  56 IVLSSAVHGNETAPIEICDAFIQDviLQRIELAHRV-LFIFGNPKSINIAERFVDE---NLNRLFNGaheNRTDNPEQIR 131
Cdd:cd06230    1 LLILAGVHGDEYEGVEAIRRLLAE--LDPSELKGTVvLVPVANPPAFEAGTRYTPLdglDLNRIFPG---DPDGSPTERL 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 132 AAKLESYVSDFftsvpegrYRCHYDLHTAIRGSknekFAVYPFLHGKPWKKSQLQFMLACGVNTILMMRS---AATTFSY 208
Cdd:cd06230   76 AHELTELILKH--------ADALIDLHSGGTGR----LVPYAILDYDSDAREKSRELARAFGGTPVIWGGdppGGTPVAA 143

                        170
                 ....*....|....*..
gi 908744126 209 FSSfqFGADAFTVELGQ 225
Cdd:cd06230  144 ARS--AGIPAITVELGG 158
Peptidase_M14_like cd00596
M14 family of metallocarboxypeptidases and related proteins; The M14 family of ...
 56-234 1.50e-08

M14 family of metallocarboxypeptidases and related proteins; The M14 family of metallocarboxypeptidases (MCPs), also known as funnelins, are zinc-binding carboxypeptidases (CPs) which hydrolyze single, C-terminal amino acids from polypeptide chains, and have a recognition site for the free C-terminal carboxyl group, which is a key determinant of specificity. Two major subfamilies of the M14 family, defined based on sequence and structural homology, are the A/B and N/E subfamilies. Enzymes belonging to the A/B subfamily are normally synthesized as inactive precursors containing preceding signal peptide, followed by an N-terminal pro-region linked to the enzyme; these proenzymes are called procarboxypeptidases. The A/B enzymes can be further divided based on their substrate specificity; Carboxypeptidase A-like (CPA-like) enzymes favor hydrophobic residues while carboxypeptidase B-like (CPB-like) enzymes only cleave the basic residues lysine or arginine. The A forms have slightly different specificities, with Carboxypeptidase A1 (CPA1) preferring aliphatic and small aromatic residues, and CPA2 preferring the bulky aromatic side chains. Enzymes belonging to the N/E subfamily enzymes are not produced as inactive precursors and instead rely on their substrate specificity and subcellular compartmentalization to prevent inappropriate cleavage. They contain an extra C-terminal transthyretin-like domain, thought to be involved in folding or formation of oligomers. MCPs can also be classified based on their involvement in specific physiological processes; the pancreatic MCPs participate only in alimentary digestion and include carboxypeptidase A and B (A/B subfamily), while others, namely regulatory MCPs or the N/E subfamily, are involved in more selective reactions, mainly in non-digestive tissues and fluids, acting on blood coagulation/fibrinolysis, inflammation and local anaphylaxis, pro-hormone and neuropeptide processing, cellular response and others. Another MCP subfamily, is that of succinylglutamate desuccinylase /aspartoacylase, which hydrolyzes N-acetyl-L-aspartate (NAA), and deficiency in which is the established cause of Canavan disease. Another subfamily (referred to as subfamily C) includes an exceptional type of activity in the MCP family, that of dipeptidyl-peptidase activity of gamma-glutamyl-(L)-meso-diaminopimelate peptidase I which is involved in bacterial cell wall metabolism.


Pssm-ID: 349427 [Multi-domain]  Cd Length: 216  Bit Score: 54.39  E-value: 1.50e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  56 IVLSSAVHGNETAPIEICDAFIQDVILQRIELAH-------RVLFIFG-NPKSINIAERF--------VDenLNRLF--- 116
Cdd:cd00596    1 ILITGGIHGNEVIGVELALALIEYLLENYGNDPLkrlldnvELWIVPLvNPDGFARVIDSggrknangVD--LNRNFpyn 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 117 NGAHENRTDNPEQIRAAKLES-----YVSDFFTSVpegRYRCHYDLHTAIrgskneKFAVYPFLH----GKPW------- 180
Cdd:cd00596   79 WGKDGTSGPSSPTYRGPAPFSepetqALRDLAKSH---RFDLAVSYHSSS------EAILYPYGYtnepPPDFsefqela 149

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 908744126 181 -KKSQLQFMLACGVNTILMMRSAATTFSYFSSFQFGADAFTVELGQVKPFGQNDM 234
Cdd:cd00596  150 aGLARALGAGEYGYGYSYTWYSTTGTADDWLYGELGILAFTVELGTADYPLPGTL 204
PRK02259 PRK02259
aspartoacylase; Provisional
 54-159 2.02e-07

aspartoacylase; Provisional


Pssm-ID: 235019  Cd Length: 288  Bit Score: 51.80  E-value: 2.02e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  54 KDIVLSSAVHGNETAPIEICDAFIQ-DVILQRIELAhrVLFIFGNPKSINIAERFVDENLNRLFNGAHENRTDNP--EQI 130
Cdd:PRK02259   3 NRVAIVGGTHGNEITGIYLVKKWQQqPNLINRKGLE--VQTVIGNPEAIEAGRRYIDRDLNRSFRLDLLQNPDLSgyEQL 80

                         90       100       110
                 ....*....|....*....|....*....|.
gi 908744126 131 RAAKLesyvSDFFTsvPEGRYRCHY--DLHT 159
Cdd:PRK02259  81 RAKEL----VQQLG--PKGNSPCDFiiDLHS 105
M14_ASTE_ASPA_like cd18430
Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally ...
 61-135 7.37e-06

Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349486 [Multi-domain]  Cd Length: 168  Bit Score: 45.51  E-value: 7.37e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 908744126  61 AVHGNETAPIEICDAfIQDVILQRIELAHRVLFIFGNPKSINIAERFVDENLNRLFNGaHENRTDNpEQIRAAKL 135
Cdd:cd18430    6 AVHGNETCGTRAVER-LLAELPSGALQKGPVTLVPANERAYAEGVRFCEEDLNRVFPG-DPDPDTY-ERRLANRL 77
M14_ASPA cd06909
Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the ...
 54-159 2.07e-05

Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the Succinylglutamate desuccinylase/aspartoacylase subfamily of the M14 family of metallocarboxypeptidases. ASPA (also known as aminoacylase 2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349480  Cd Length: 190  Bit Score: 44.51  E-value: 2.07e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  54 KDIVLSSAVHGNEtapieICDAFIQDVILQRIELAHR----VLFIFGNPKSINIAERFVDENLNRLFngAHENRTDNP-- 127
Cdd:cd06909    1 KRVAIVGGTHGNE-----LTGVYLVKHWLKNPELIERksfeVHPLLANPRAVEQCRRYIDTDLNRCF--SLENLSSAPss 73

                         90       100       110
                 ....*....|....*....|....*....|....*..
gi 908744126 128 ---EQIRAAKLESYVsdfftsVPEGRYRCHY--DLHT 159
Cdd:cd06909   74 lpyEVRRAREINQIL------GPKGNPACDFiiDLHN 104
M14_ASTE_ASPA-like cd06251
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 52-247 3.17e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349469 [Multi-domain]  Cd Length: 195  Bit Score: 40.99  E-value: 3.17e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  52 GDKD---IVLSSAVHGNETAPIEIcdafIQDVI--LQRIELAHRVLFIFG-NPKSINIAERFV---DENLNRLFNGaHEN 122
Cdd:cd06251    8 GAKPgptLLLTAAIHGDELNGIEV----IQRLLedLDPSKLRGTLIAIPVvNPLGFENNSRYLpddGRDLNRSFPG-SEK 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 123 RTDnPEQIrAAKLesyvsdfFTSVPEgryRCHY--DLHTAIRGSKNekfavYPFLHGKpWKKSQLQFML-ACGVNTIL-- 197
Cdd:cd06251   83 GSL-ASRL-AHLL-------WNEIVK---KADYviDLHTASTGRTN-----LPYVRAD-LRDPESRRMAeAFGAPVIVdd 144

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 908744126 198 -----MMRSAATtfsyfssfQFGADAFTVELGQVKPFGQNDMSRF-SAVKQTLKAL 247
Cdd:cd06251  145 pgedgSLRGAAV--------ELGIPAITVELGEALRFDEDIIRRGvEGVLNVLRHL 192
M14_ASTE_ASPA-like cd06910
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 55-129 4.79e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349481  Cd Length: 208  Bit Score: 40.80  E-value: 4.79e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  55 DIVLSSAVHGNEtapieICDAFIQDVILQRI--ELAHRVLFIFGNPKSIN-------IAERFVDENLNRLFNGAhenRTD 125
Cdd:cd06910   26 HVMINALTHGNE-----ICGAIALDWLLKNGvrPLRGRLTFCFANVEAYErfdparpTASRFVDEDLNRVWGPE---LLD 97


                 ....
gi 908744126 126 NPEQ 129
Cdd:cd06910   98 GPEQ 101
M14_ASTE_ASPA-like cd06254
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 54-225 9.12e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349472  Cd Length: 198  Bit Score: 39.87  E-value: 9.12e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  54 KDIVLSSAVHGNETAPIEICDAFIQDviLQRIELAHRVLFI--------FGNPKSINiaerFVDE-NLNRLFNGaheNRT 124
Cdd:cd06254   12 PTLLITAGIHGGEYPGILAAIRLARE--LDPADVKGTLIIVhianvsgfEARTPFVV----PEDGkNLNRVFPG---DPD 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 125 DNP-EQIrAAKLESYV---SDFftsvpegryrcHYDLHTairGSKNEK---FAVYPFLHGKPWKKSQLQFMLACGVNTIL 197
Cdd:cd06254   83 GTLtERI-AYFLTREIisrADF-----------LIDLHG---GDANEAltpFVYYPGGASEEVNDISRAAAQALGLPYIV 147

                        170       180
                 ....*....|....*....|....*...
gi 908744126 198 MMRSAATTFSYFSSFQFGADAFTVELGQ 225
Cdd:cd06254  148 ISSSEKGTGYYSYAALRGIPSILVERGG 175
M14_ASTE_ASPA-like cd06253
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 50-224 2.47e-03

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349471  Cd Length: 211  Bit Score: 38.73  E-value: 2.47e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  50 TYGDKDIVLSSAVHGNETAPIEICDA---FIQDVILQRIELAHRVLFI-FGNPKSINIAERFV---DENLNRLFNGAHEN 122
Cdd:cd06253   19 GNAEPRIAIVAGIHGDELNGLYVCSRlirFLKELEEGGYKLKGKVLVIpAVNPLGINSGTRFWpfdNLDMNRMFPGYNKG 98

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 123 RTdnPEQIrAAKLESYVSDfftsvpegrYRCHYDLHTA---------IRGSKNEKFAVYPflhgkpwkkSQLQFMLacgv 193
Cdd:cd06253   99 ET--TERI-AAALFEDLKG---------ADYGIDLHSSndflreipqVRVIESGAQDLLP---------LAKFLGL---- 153

                        170       180       190
                 ....*....|....*....|....*....|....*
gi 908744126 194 nTILMMRSAAT----TFSYfSSFQFGADAFTVELG 224
Cdd:cd06253  154 -DVVWVHPASTvdtgTLAY-NWNEWGTKALVLEMG 186
M14_ASTE_ASPA_like cd18174
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 58-225 3.65e-03

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349484  Cd Length: 187  Bit Score: 37.99  E-value: 3.65e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126  58 LSSAVHGNETAPIEICDAFIQD---------VILqrIELAHRVLF----IFGNPksiniaerfVD-ENLNRLFNGaHENR 123
Cdd:cd18174    3 VTAGVHGYEYASIEALQRLIKEldpaklsgtVIV--VPIANIPAFegrsIYVNP---------LDgKNLNRSFPG-DPDG 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 908744126 124 TdNPEQIRAAKLESYVSdfftsvpegryRCHY--DLHTairGSKNEK---FAVYPFLHGKPWKKSQLQFMLACGVNTILM 198
Cdd:cd18174   71 T-PTERLAHWLTTNVIA-----------RADYyiDLHG---GDLNEDlrpFVYYYETGNAALDAASREMAEAFGLDHIVF 135

                        170       180       190
                 ....*....|....*....|....*....|..
gi 908744126 199 -----MRSAATTFSYFSSFQFGADAFTVELGQ 225
Cdd:cd18174  136 ykarlKASRGSLYTQAAALLRGIPAILVEAGG 167
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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