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Conserved domains on  [gi|86278336|gb|ABC88420|]
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MazE [Delivery vector pIEF16S]

Protein Classification

type II toxin-antitoxin system antitoxin MazE( domain architecture ID 10013344)

type II toxin-antitoxin system antitoxin MazE binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity

CATH:  2.10.260.10
Gene Ontology:  GO:0003677|GO:0006355|GO:0097351|GO:0044877
SCOP:  4000397

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK09798 PRK09798
MazF-MazE toxin-antitoxin system antitoxin MazE;
1-82 2.56e-49

MazF-MazE toxin-antitoxin system antitoxin MazE;


:

Pssm-ID: 182082  Cd Length: 82  Bit Score: 150.24  E-value: 2.56e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86278336   1 MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE 80
Cdd:PRK09798  1 MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE 80


                ..
gi 86278336  81 VW 82
Cdd:PRK09798 81 VW 82
 
Name Accession Description Interval E-value
PRK09798 PRK09798
MazF-MazE toxin-antitoxin system antitoxin MazE;
1-82 2.56e-49

MazF-MazE toxin-antitoxin system antitoxin MazE;


Pssm-ID: 182082  Cd Length: 82  Bit Score: 150.24  E-value: 2.56e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86278336   1 MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE 80
Cdd:PRK09798  1 MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE 80


                ..
gi 86278336  81 VW 82
Cdd:PRK09798 81 VW 82
MazE COG2336
Antitoxin component MazE of the MazEF toxin-antitoxin module [Defense mechanisms];
3-82 3.05e-29

Antitoxin component MazE of the MazEF toxin-antitoxin module [Defense mechanisms];


Pssm-ID: 441907  Cd Length: 80  Bit Score: 99.19  E-value: 3.05e-29
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86278336  3 HSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKePVFTLAELVNDITPENLHENIDWGEPKDKEVW 82
Cdd:COG2336  2 TTKIRKWGNSLGVRIPKALLEQLGLKEGDEVELEVEDGRIVIRPVRR-PRYTLEELLAGITPENAHEEYDWGKPVGKEVW 80
SpoVT_AbrB smart00966
SpoVT / AbrB like domain; This domain is found in AbrB from Bacillus subtilis. The product of ...
 7-51 2.04e-10

SpoVT / AbrB like domain; This domain is found in AbrB from Bacillus subtilis. The product of the abrB gene is an ambiactive repressor and activator of the transcription of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. AbrB is thought to interact directly with the transcription initiation regions of genes under its control. AbrB contains a helix-turn-helix structure, but this domain ends before the helix-turn-helix begins. The product of the B. subtilis gene spoVT is another member of this family and is also a transcriptional regulator. DNA-binding activity in this AbrB homologue requires hexamerisation. Another family member has been isolated from the Sulfolobus solfataricus and has been identified as a homologue of bacterial repressor-like proteins. The Escherichia coli family member SohA or Prl1F appears to be bifunctional and is able to regulate its own expression as well as relieve the export block imposed by high-level synthesis of beta-galactosidase hybrid proteins.


Pssm-ID: 198034 [Multi-domain]  Cd Length: 45  Bit Score: 50.58  E-value: 2.04e-10
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 86278336     7 KRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEP 51
Cdd:smart00966  1 VKVGNSGQVTIPKEVREKLGIKEGDEVELEVDGDGIILEPVKKAP 45
MazE_antitoxin pfam04014
Antidote-toxin recognition MazE, bacterial antitoxin; AbrB-like is a family of small proteins ...
 7-50 1.92e-09

Antidote-toxin recognition MazE, bacterial antitoxin; AbrB-like is a family of small proteins that operate in conjunction with a cognate toxin molecule. The commonly attributed role of toxin-antitoxin systems is to maintain low-copy number plasmids from one generation to the next. Such gene-pairs are also found on chromosomes and to be associated with a number of biological functions such as: reduction of protein synthesis, gene regulation and retardation of cell growth under nutritional stress. This family includes proteins from a number of different pairings, eg MazE, AbrB, VapB, PhoU, PemI-like and SpoVT. MazE is the antidote to the toxin MazF of E. coli. MazE-MazF in E. coli is a regulated prokaryotic chromosomal addiction module. MazE antidote is degraded by the ClpPA protease of the bacterial proteasome. MazE-MazF is thought to play a role in programmed cell death when cells suffer nutrient deprivation, and MazE-MazF modules have also been implicated in the bacteriostatic effects of other addiction modules.


Pssm-ID: 397909 [Multi-domain]  Cd Length: 44  Bit Score: 47.89  E-value: 1.92e-09
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 86278336    7 KRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKE 50
Cdd:pfam04014  1 FKVGNSGAVRIPKEVRKALGIKEGDEVELEVDGDKIILKPKKSE 44
 
Name Accession Description Interval E-value
PRK09798 PRK09798
MazF-MazE toxin-antitoxin system antitoxin MazE;
1-82 2.56e-49

MazF-MazE toxin-antitoxin system antitoxin MazE;


Pssm-ID: 182082  Cd Length: 82  Bit Score: 150.24  E-value: 2.56e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86278336   1 MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE 80
Cdd:PRK09798  1 MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE 80


                ..
gi 86278336  81 VW 82
Cdd:PRK09798 81 VW 82
MazE COG2336
Antitoxin component MazE of the MazEF toxin-antitoxin module [Defense mechanisms];
3-82 3.05e-29

Antitoxin component MazE of the MazEF toxin-antitoxin module [Defense mechanisms];


Pssm-ID: 441907  Cd Length: 80  Bit Score: 99.19  E-value: 3.05e-29
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86278336  3 HSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKePVFTLAELVNDITPENLHENIDWGEPKDKEVW 82
Cdd:COG2336  2 TTKIRKWGNSLGVRIPKALLEQLGLKEGDEVELEVEDGRIVIRPVRR-PRYTLEELLAGITPENAHEEYDWGKPVGKEVW 80
SpoVT_AbrB smart00966
SpoVT / AbrB like domain; This domain is found in AbrB from Bacillus subtilis. The product of ...
 7-51 2.04e-10

SpoVT / AbrB like domain; This domain is found in AbrB from Bacillus subtilis. The product of the abrB gene is an ambiactive repressor and activator of the transcription of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. AbrB is thought to interact directly with the transcription initiation regions of genes under its control. AbrB contains a helix-turn-helix structure, but this domain ends before the helix-turn-helix begins. The product of the B. subtilis gene spoVT is another member of this family and is also a transcriptional regulator. DNA-binding activity in this AbrB homologue requires hexamerisation. Another family member has been isolated from the Sulfolobus solfataricus and has been identified as a homologue of bacterial repressor-like proteins. The Escherichia coli family member SohA or Prl1F appears to be bifunctional and is able to regulate its own expression as well as relieve the export block imposed by high-level synthesis of beta-galactosidase hybrid proteins.


Pssm-ID: 198034 [Multi-domain]  Cd Length: 45  Bit Score: 50.58  E-value: 2.04e-10
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 86278336     7 KRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEP 51
Cdd:smart00966  1 VKVGNSGQVTIPKEVREKLGIKEGDEVELEVDGDGIILEPVKKAP 45
MazE_antitoxin pfam04014
Antidote-toxin recognition MazE, bacterial antitoxin; AbrB-like is a family of small proteins ...
 7-50 1.92e-09

Antidote-toxin recognition MazE, bacterial antitoxin; AbrB-like is a family of small proteins that operate in conjunction with a cognate toxin molecule. The commonly attributed role of toxin-antitoxin systems is to maintain low-copy number plasmids from one generation to the next. Such gene-pairs are also found on chromosomes and to be associated with a number of biological functions such as: reduction of protein synthesis, gene regulation and retardation of cell growth under nutritional stress. This family includes proteins from a number of different pairings, eg MazE, AbrB, VapB, PhoU, PemI-like and SpoVT. MazE is the antidote to the toxin MazF of E. coli. MazE-MazF in E. coli is a regulated prokaryotic chromosomal addiction module. MazE antidote is degraded by the ClpPA protease of the bacterial proteasome. MazE-MazF is thought to play a role in programmed cell death when cells suffer nutrient deprivation, and MazE-MazF modules have also been implicated in the bacteriostatic effects of other addiction modules.


Pssm-ID: 397909 [Multi-domain]  Cd Length: 44  Bit Score: 47.89  E-value: 1.92e-09
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 86278336    7 KRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKE 50
Cdd:pfam04014  1 FKVGNSGAVRIPKEVRKALGIKEGDEVELEVDGDKIILKPKKSE 44
PRK11347 PRK11347
antitoxin ChpS; Provisional
 5-82 2.96e-09

antitoxin ChpS; Provisional


Pssm-ID: 183094  Cd Length: 83  Bit Score: 48.89  E-value: 2.96e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86278336   5 SVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEpvFTLAELVN--DITPENLHENIDWGE--PKDKE 80
Cdd:PRK11347  4 TIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRR--YSLDELLAqcDMNAAELSEQDVWGKstPAGDE 81


                ..
gi 86278336  81 VW 82
Cdd:PRK11347 82 IW 83
VagC COG4456
Virulence-associated protein VagC (function unknown) [Function unknown];
10-49 3.30e-03

Virulence-associated protein VagC (function unknown) [Function unknown];


Pssm-ID: 443554  Cd Length: 74  Bit Score: 32.83  E-value: 3.30e-03
                       10        20        30        40
               ....*....|....*....|....*....|....*....|
gi 86278336 10 GNSPAVRIPAtlmqALNLNiDDEVKIDLVDGKLIIEPVRK 49
Cdd:COG4456  9 GRSQAVRLPK----EFRFP-GDEVEIRREGDTLILRPKPK 43
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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